Biosensors and Bioelectronics: X最新文献

{"title":"Simple and sensitive method for in vitro monitoring of red blood cell viscoelasticity by Quartz Crystal Microbalance with dissipation monitoring (QCM-D)","authors":"Simonetta Palleschi ,&nbsp;Leopoldo Silvestroni ,&nbsp;Barbara Rossi ,&nbsp;Simone Dinarelli ,&nbsp;Marco Magi ,&nbsp;Lorenzo Giacomelli ,&nbsp;Andrea Bettucci","doi":"10.1016/j.biosx.2024.100554","DOIUrl":"10.1016/j.biosx.2024.100554","url":null,"abstract":"<div><div>Viscoelasticity (VE) is the intrinsic mechano-dynamic property enabling red blood cells (RBCs) to undergo prompt and repeated deformations while maintaining structural integrity. Assessing RBC VE and how different stressors can affect it is of great interest. Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) is a technology exploiting high-frequency acoustic waves to probe soft matter rheological properties. In the present study, a QCM-D method is reported for <em>in vitro</em> monitoring of cell VE in viable RBCs. The method is based on casting a sensor-adherent cell monolayer and modeling it as an effective viscoelastic medium, and allows to extrapolate proxy values of both the elastic and the viscous cell shear moduli. Real-time VE changes induced by the known cell VE stressors temperature, medium tonicity, glutaraldehyde, methyl-β-cyclodextrin and cytochalasin D have been reliably identified. The method is relatively simple and inexpensive, non-invasive, and able to seize subtle changes of cell biomechanics. Hence, it could be usefully exploited for <em>in vitro</em> assessment of RBC rheological properties and their alterations induced by external chemico-physical stimuli.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100554"},"PeriodicalIF":10.61,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted biosensors for intracellular lipid droplet content detection","authors":"Shengdong Li","doi":"10.1016/j.biosx.2024.100551","DOIUrl":"10.1016/j.biosx.2024.100551","url":null,"abstract":"<div><div>Lipid droplets participate in the metabolic process of many living cells, and their abnormalities are highly correlated with many diseases such as fatty liver, diabetes, and cancer. Nevertheless, the current methods are inadequate for elucidating the relationship between diverse diseases and lipid droplets. In particular, the visualization of tumors with abnormal distribution of lipid droplets remains a significant challenge. Therefore, a water-soluble biosensor with lipid droplet specificity is synthesized by copolymerization of coumarin derivatives with low toxicity and high hydrophilic hydroxyl terminal polyethylene glycol. The survival rate of HeLa cells cultured with lipid drop biosensor for 24 h was more than 90% on average, and the toxicity was less. The co-localization imaging experiments with lipid droplets showed that the biosensor had a good ability to target lipid droplets in HeLa cells. The experimental results of the response of different cell lines to the biosensor showed that the tumor cells had a good response and uptake ability. These results indicate that the prepared biosensor has a good distinguishing function between normal cells and tumor cells, and provides an experimental basis for the early diagnosis of clinical tumors.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100551"},"PeriodicalIF":10.61,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intact photosynthetic bacteria-based electrodes for self-powered metal ions monitoring","authors":"Jefferson Honorio Franco ,&nbsp;Paolo Stufano ,&nbsp;Rossella Labarile ,&nbsp;Dario Lacalamita ,&nbsp;Pierluigi Lasala ,&nbsp;Elisabetta Fanizza ,&nbsp;Massimo Trotta ,&nbsp;Gianluca Maria Farinola ,&nbsp;Matteo Grattieri","doi":"10.1016/j.biosx.2024.100552","DOIUrl":"10.1016/j.biosx.2024.100552","url":null,"abstract":"<div><div>The low-cost and early monitoring of metal ion contaminants is paramount to prevent widespread contamination of water environments. Self-powered microbial electrochemical sensors represent an interesting approach to achieving this goal. Purple non-sulfur bacteria have a versatile metabolism and a well-characterized photosynthetic system, making them an ideal candidate for developing biohybrid technologies. In this work, we report the use of these bacteria in biophotoelectrodes to develop self-powered monitoring systems for two common pollutants, NiCl₂ and CuSO₄. The microbial biophotoelectrode was obtained on a homemade poly-hydroxybutyrate-carbon nanofibers electrode modified with a redox-adhesive polydopamine matrix-based entrapping the purple bacterium <em>Rhodobacter capsulatus</em>. The presence of 500 μM NiCl₂ resulted in a 60 % decrease in current density, while the simultaneous presence of 100 μM NiCl₂ and 100 mM CuSO₄ led to an 83 % current inhibition. Given the implementation of the biophotoelectrode in the field, the biohybrid system was tested in a complex matrix containing beer, demonstrating the promising ability of the photoelectrochemical system to act as an efficient biosensor in complex solutions. Finally, the biohybrid electrode was coupled to a cathode performing oxygen reduction, which allowed obtaining a self-powered monitoring system, paving the way for the future implementation of a low-cost monitoring system for widespread metal ions contaminant monitoring.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100552"},"PeriodicalIF":10.61,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach to tyrosinase-based biosensors: Electrode reactions and biological measurement","authors":"Masumeh Goodarzi ,&nbsp;Hosna Tavakoli ,&nbsp;Maryam Arab Khalaj ,&nbsp;Hassan Tavakoli","doi":"10.1016/j.biosx.2024.100550","DOIUrl":"10.1016/j.biosx.2024.100550","url":null,"abstract":"<div><div>This study introduces a novel tyrosinase-based biosensor designed to simultaneously detect tyrosinase, tyrosine, L-DOPA, and L-DOPA quinone. The biosensor, designated as GCE/GNP/Cys/Chit/Tyrase, was developed by immobilizing tyrosinase on a modified glassy carbon electrode (GCE) that incorporates electrodeposited gold nanoparticles, cysteine, and chitosan. The morphology of the biosensor was characterized using scanning electron microscopy (SEM). The electrochemical behaviors of the biosensor were explored in response to the target analytes. Key analytical characteristics were assessed, including linear range, sensitivity, selectivity, limits of detection and quantification, long-term stability, repeatability, reproducibility, electrochemically active surface area, and charge transfer behavior. The biosensor demonstrated a linear response range of 1–120 μM, with an impressive sensitivity of 200.4 mA.Lmol⁻¹cm⁻² and a detection limit of 27 μM. This study provides a comprehensive evaluation of the analytical features of tyrosinase-based biosensors. Additionally, the biosensor was applied to quantify dopamine in brain tissue, utilizing a calibration curve derived from the fabricated biosensor. The dopamine concentration measured in five Wistar rats was 35 ± 2.75 μM, reflecting the mean and standard deviation, respectively. These results confirm the biosensor's capability for accurately detecting this critical neurotransmitter in the brains of Wistar rats. This investigation underscores the potential of tyrosinase-based biosensors for diverse analytical applications in biological samples.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100550"},"PeriodicalIF":10.61,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electroactive microfluidic platform integrated with AM-pDEP focusing and side-counter design for selective cell sorting and single-cell quantification","authors":"Zuyuan Tian ,&nbsp;Mohamed Shaheen ,&nbsp;Tianxiang Jiang ,&nbsp;Shaoxi Wang ,&nbsp;Xihua Wang ,&nbsp;Jie Chen","doi":"10.1016/j.biosx.2024.100549","DOIUrl":"10.1016/j.biosx.2024.100549","url":null,"abstract":"<div><div>Rapid isolation and precise quantification of target cancer cells are crucial for precision medicine applications. Conventional fluorescence-based methods require bulky optical instrumentation and specialized expertise. Recent advances in label-free microfluidic techniques have attempted to overcome these limitations but often suffer from reduced reliability and throughput when integrating multiple functions. Here, we demonstrate an electroactive microfluidic platform that integrates functions of selective concentration, particle focusing, and single-cell level quantification without introducing additional complex physical microstructures. Our device employs the dielectrophoresis (DEP) effect combined with an interdigitated cell sorter to achieve selective cell concentration. For quantification and characterization, the device leverages the impedimetric Coulter principle to achieve precise particle counting and characterization at single-cell resolution. To address the inherent tradeoff between sensitivity and throughput in the traditional Coulter counter, we utilize the tilted interdigitated electrode configuration excited by an amplitude-modulated (AM) Positive DEP (pDEP) signal instead of the commonly employed narrow channel or sheath flow to realize the effective focusing and alignment of the cell stream. The focusing module is combined with our side-counter design to detect cells. The performance of our device and its embedded modules has been verified with mixed breast cancer and blood model cell lines, where high consistency between optical and electrical detection has been demonstrated. We expect the integration of the proposed AM-pDEP focusing approach and the corresponding microfluidic design for cell sorter and on-chip flow cytometry would offer an alternative engineering solution to the stream-based on-chip single-cell analysis where reliable cell focusing and single-cell sensing are required.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100549"},"PeriodicalIF":10.61,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion-selective electrode based on polyurethane-immobilized di-(2-ethyl hexyl) phosphoric acid for low-concentration aqueous Pb2+ detection and quantification","authors":"Khairun Nisah ,&nbsp;Eka Safitri ,&nbsp;Rahmi Rahmi ,&nbsp;Muliadi Ramli ,&nbsp;Reni Silvia Nasution ,&nbsp;Muhammad Iqhrammullah","doi":"10.1016/j.biosx.2024.100547","DOIUrl":"10.1016/j.biosx.2024.100547","url":null,"abstract":"<div><div>This study used a polyurethane (PU)-based ion selective electrode (ISE) immobilized with di-(2-ethyl hexyl) phosphoric acid (D2EHPA) to detect and quantify Pb²⁺ ions at low concentrations (10⁻¹⁰ to 10⁻¹ M) in aqueous solution. PU, synthesized from castor oil (<em>Ricinus communis</em> L.), was utilized as the ISE membrane matrix. The ISE demonstrated a sensitivity of 26.24 ± 0.12 mV/decade, a detection limit of 1.44 × 10⁻⁷ M, and a response time of 10 s. We maintained the measurement stability for up to six days of storage.</div><div>The results of Pb²⁺ quantification produced by ISE were compared with the results using atomic absorption spectroscopy (AAS), indicating similar Pb²⁺ concentrations in both artificial and real wastewater samples (calculated t-value &lt; theoretical t-value). Therefore, this ISE is suitable for detecting trace levels of Pb²⁺ and quantifying them with high accuracy.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100547"},"PeriodicalIF":10.61,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resting-state brain network analysis and applied evaluation of global developmental delay in preterm infants: A functional near-infrared spectroscopic study","authors":"Hengye Huang ,&nbsp;Karol Kexin Sun ,&nbsp;Dan Wu ,&nbsp;Chuncao Zhang ,&nbsp;Yanyan Huo ,&nbsp;Yuan Tian ,&nbsp;Guangjun Yu","doi":"10.1016/j.biosx.2024.100546","DOIUrl":"10.1016/j.biosx.2024.100546","url":null,"abstract":"<div><div>Global developmental delay (GDD) is a common neurodevelopmental disorder in children under 5 years of age. This study assessed the potential of functional Near-Infrared Spectroscopy (fNIRS) technology aiding diagnosis of GDD in premature infants and explored brain pathogenesis of GDD. This prospective cohort study was conducted between December 2020 and June 2023 among preterm infants in five hospitals in China. The primary outcome was the diagnosis of GDD. A 5-min fNIRS brain scan was performed in preterm infants at 4-, 8-, and 12-month corrected age in two hospitals. A diagnostic model based on fNIRS brain network and an integrated diagnostic model built on fNIRS data as well as birth head circumference was developed. Diagnostic models were assessed using the area under the curve (AUC) from receiver operating characteristic curves. Brain network analysis revealed significantly worse left-sided functional connectivity in GDD preterm infants. The fNIRS technique had satisfactory diagnostic value for GDD in all age groups. The diagnostic accuracy of the combined diagnostic model significantly improved. This study suggested that fNIRS could be considered for early screening and late auxiliary diagnosis of GDD in preterm infants. Furthermore, it can also be used to explore GDD brain pathogenesis.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100546"},"PeriodicalIF":10.61,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142653619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advancements in nanozyme hydrogel based system for enhanced sensing applications","authors":"Simran Makkar ,&nbsp;Nikita Sarawagi ,&nbsp;Nitesh Priyadarshi ,&nbsp;Sunaina Kaul ,&nbsp;Palakjot Kour Sodhi ,&nbsp;Ajay Kumar Srivastava ,&nbsp;Nitin Kumar Singhal","doi":"10.1016/j.biosx.2024.100548","DOIUrl":"10.1016/j.biosx.2024.100548","url":null,"abstract":"<div><div>In recent years, combining hydrogels with nanozymes has shown promise as a platform for sensing applications. Nanozymes are simple, affordable, and stable alternatives that use enzyme-like capabilities. Conversely, hydrogels provide hydrated, biocompatible environments, which enhance the stability and activity of nanozymes. The most recent advancements in nanozyme-hydrogel hybrid sensing systems are examined in this study, with a focus on their enzymatic activities—peroxidase and oxidase mimetics—and how they can be used to detect a variety of analytes, such as glucose, pesticide, and many more.</div><div>In addition to simulating the biological environment, the synergistic combination of hydrogels and nanozyme improves the biosensor's sensitivity, specificity, and fast reaction times. These hybrid systems also provide an extensible framework for integrating multiple sensory modalities into a single functional device. This review highlights the groundbreaking potential and uses of hydrogel sensors based on nanozymes in environmental and medical diagnostics and potential future developments.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100548"},"PeriodicalIF":10.61,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-enhanced nanozyme sensors: Colorimetric and fluorescence sensing techniques","authors":"Tileshwar Sahare,&nbsp;Nandini Singh,&nbsp;Badri Narayana Sahoo,&nbsp;Abhijeet Joshi","doi":"10.1016/j.biosx.2024.100544","DOIUrl":"10.1016/j.biosx.2024.100544","url":null,"abstract":"<div><div>The advent of smartphone technology has changed the biosensing field, especially by integrating nanozyme-based colorimetric, fluorescence sensing strategy. This review article describes the development of smartphone-assisted nanozyme sensor technology, harboring increased sensitivity and specificity for detecting relevant markers. While mimicking natural catalytic enzymes, nanozymes show exceptional performance in a variety of analytical applications. Combining the computing power and imaging capabilities of smartphones, nanozyme based sensors offer a low-cost, user-friendly, and scalable solution for point-of-care technology. We have discussed the principles behind colorimetric and fluorescence sensing techniques, the role of nanozymes in these processes, and the methodologies for leveraging smartphone technology for data acquisition and analysis. Crucial applications in medical diagnostics, environmental monitoring, and food safety are reviewed, with a focus on recent innovations and forthcoming prospects. The convergence of nanozyme sensors and smartphone technology promises to democratize access to advanced colorimetric or fluorometric based biosensing.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100544"},"PeriodicalIF":10.61,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in gluten detection: A rapid colorimetric approach using core-satellite magnetic particles","authors":"Daniele Marra ,&nbsp;Adriano Acunzo ,&nbsp;Andrea Fulgione ,&nbsp;Maria De Luca ,&nbsp;Reynaldo Villalonga ,&nbsp;Francesco Pisani ,&nbsp;Loredana Biondi ,&nbsp;Federico Capuano ,&nbsp;Raffaele Velotta ,&nbsp;Bartolomeo Della Ventura ,&nbsp;Vincenzo Iannotti","doi":"10.1016/j.biosx.2024.100545","DOIUrl":"10.1016/j.biosx.2024.100545","url":null,"abstract":"<div><div>Monitoring gluten levels in foods labelled as gluten-free or low-gluten — i.e. containing less than 20 ppm and 100 ppm of gluten, respectively — is crucial for preventing celiac disease-related disorders. Due to their inherent complexity, the standard analytical techniques to assess the gluten content in food are not suitable for on-site measurements, whose need is widely recognized. In this context, we developed a rapid and cost-effective biosensor based on core-satellite magnetic particles (CSMPs) — magnetic cores coated with gold nanoparticles (AuNPs) — further functionalized with anti-gliadin antibodies. The study demonstrates that a 0.016% concentration of the surfactant Tween-20 can induce the spontaneous formation of stable CSMP clusters in dispersion. These clusters, composed of weakly interacting functionalized CSMPs, undergo fragmentation in the presence of gliadin, which specifically binds to the antibodies on the CSMPs. This process results in a colour change, which is measurable by a UV–VIS spectrophotometer. Gliadin extraction was achieved by treating the sample with a non-toxic ethanol-water mixture (60%), sufficient to induce a measurable colour change in the presence of gluten contamination, with a limit of detection (LOD) of 8 ppm, which is lower than low limit established for gluten-free food.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100545"},"PeriodicalIF":10.61,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024001092/pdfft?md5=96b1a1a59d87700aead103072aaa89c9&pid=1-s2.0-S2590137024001092-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}