Biosensors and Bioelectronics: X最新文献_第3页

Nanostructured immunosensing system for label-free impedimetric detection of multiple breast cancer biomarkers (CEA and HER2) using CoMoO4@PANI-PPy Nanocomposite

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-20 DOI: 10.1016/j.biosx.2025.100596

Omar Ramadan, Rabeay Y.A. Hassan

{"title":"Nanostructured immunosensing system for label-free impedimetric detection of multiple breast cancer biomarkers (CEA and HER2) using CoMoO4@PANI-PPy Nanocomposite","authors":"Omar Ramadan, Rabeay Y.A. Hassan","doi":"10.1016/j.biosx.2025.100596","DOIUrl":"10.1016/j.biosx.2025.100596","url":null,"abstract":"<div><div>Breast cancer patients often exhibit positive expression of selective tumor biomarkers including the carcinoembryonic antigen (CEA), and human epidermal growth factor receptor 2 (HER2). These potential protein biomarkers are highly crucial for the selective and early tumor diagnosis. In this study, disposable dual impedimetric immunosensing systems were constructed using the cobalt molybdate (CoMoO<sub>4</sub>) and polyaniline/polypyrrole (PANI-PPy) nanocomposite, for the rapid quantification of CEA and HER2 concentration levels. The designed sensing probes were fabricated by chemically conjugating two-targeting antibodies (anti-CEA and anti-HER2) on the nanostructured electrode surfaces modified with (AuNPs/CoMoO<sub>4</sub>@PANI-PPy). These successful chemical antibody immobilizations were enabled by the use of 4-aminothiophenol (4-ATP) as a cross-linking agent. The impedimetric immunosensing assay was fully optimized by screening of nanomaterials, determining the optimal ratio of the nanocomposite, adjusting the time of self-assembled monolayer (SAM) formation, and identifying the degree of cross-reactivity with potential non-targeting molecules. Accordingly, the biosensors demonstrated distinguished linear dynamic ranges of 1.0 fg/mL to 100 ng/mL for the CEA and 25 fg/mL to 100 ng/mL for the HER2, with detection limits of 8.2 fg/mL and 4.9 fg/mL for CEA and HER2, respectively. The immunesensor's reproducibility and stability were studied, showing minimal variation in charge transfer resistance (RSD = 1.33%, n = 5) and maintaining 82.5% functionality after four weeks of storage at 4 °C. These findings highlighted the potential use of these biosensor chips for clinical applications in early breast cancer detection and rapid diagnosis.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100596"},"PeriodicalIF":10.61,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A deeper evaluation of cytokeratin fragment 21-1 as a lung cancer tumor marker and comparison of different assays

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-17 DOI: 10.1016/j.biosx.2025.100593

Dianna J. Rowe , Timothy A. Khalil , Michael N. Kammer , Caroline M. Godfrey , Yong Zou , Cindy L. Vnencak-Jones , David Xiao , Stephen Deppen , Eric L. Grogan

{"title":"A deeper evaluation of cytokeratin fragment 21-1 as a lung cancer tumor marker and comparison of different assays","authors":"Dianna J. Rowe , Timothy A. Khalil , Michael N. Kammer , Caroline M. Godfrey , Yong Zou , Cindy L. Vnencak-Jones , David Xiao , Stephen Deppen , Eric L. Grogan","doi":"10.1016/j.biosx.2025.100593","DOIUrl":"10.1016/j.biosx.2025.100593","url":null,"abstract":"<div><div>Studies show CYFRA 21-1 fragments of cytokeratin 19 (CK19) to be promising biomarkers for non-small cell lung cancer (NSCLC). Although previous literature identifies specific CYFRA 21-1 antibody binding epitopes, the exact molecular weight of the CK19 fragment being detected by current assays is not well-documented. Serum samples from 58 patients (lung cancer (N = 36), control (N = 22)) were used to measure CYFRA 21-1 across four different quantification assays: enzyme-linked immunosorbent assay (ELISA), chemiluminescent assay (ChLIA), electrochemiluminescence immunoassay (ECLIA), and compensated interferometric reader (CIR). In the cancer group, correlation between ECLIA and ELISA was high (R<em>(Pearson)</em> = 0.948, r<em>(Spearman)</em> = 0.868) while correlation between ECLIA vs ChLIA and ECLIA vs CIR was low (R<em>=</em> 0.005, r = −0.0593), (R = 0.0275, r = 0.167), respectively. In the control group, correlation between ECLIA and ELISA was high (R = 0.948, r = 0.868) while correlation between ECLIA vs ChLIA and ECLIA vs CIR was low (R = 0.005, r = −0.0593), (R = 0.0275, r = 0.167), respectively. Compared to ECLIA, concordance coefficients <strong>(</strong><em>p</em><sub><em>c</em></sub>) were poor (<em>p</em><sub><em>c</em></sub> < 0.90) across all assays except for cancers group in ELISA (<em>p</em><sub><em>c</em></sub> = 0.913). ECLIA was the only assay to report control ranges above 1 ng/mL CYFRA 21-1 (ECLIA, 1.14–21.59 ng/mL; ELISA, 0.79–24.26 ng/mL; ChLIA, 0.062–0.691 ng/mL; 0.08–7.68 ng/mL). Differing sizes of the protein being measured by each assay may have a role in the discrepancies observed. Given the different CYFRA 21-1 concentration estimates among assays, further characterization of the fragment and its release during epithelial malignancies, such as NSCLC, is imperative to developing effective biomarker assays.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100593"},"PeriodicalIF":10.61,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A liquid biopsy algorithm exploiting local exosome heterogeneity

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-16 DOI: 10.1016/j.biosx.2025.100585

Taekmin Kim, Dayeon Choi, Soonjoung Kwon, Se-Eun Park, Jongki Kim, Yoon-Ho Lee, Seung-Cheol Choi, Se-Hwan Paek

{"title":"A liquid biopsy algorithm exploiting local exosome heterogeneity","authors":"Taekmin Kim, Dayeon Choi, Soonjoung Kwon, Se-Eun Park, Jongki Kim, Yoon-Ho Lee, Seung-Cheol Choi, Se-Hwan Paek","doi":"10.1016/j.biosx.2025.100585","DOIUrl":"10.1016/j.biosx.2025.100585","url":null,"abstract":"<div><div>We developed a novel cancer detection method that leverages changes in local exosome heterogeneity to regulate bulk-scale heterogeneity in body fluids. Using an automated, reversible isolation system and downstream immunoassays, we investigated exosome subclasses exhibiting marker composition changes associated with cancer. We introduced an index, R, to measure local heterogeneity changes, quantifying the proportion of a third marker within double-marker-positive subclasses. This method, which employs pan-exosome tetraspanins CD9, CD63, and CD81, revealed distinct heterogeneity differences between normal and cancer samples. These differences were consistently demonstrated in exosome samples derived from various cell lines and human sera. Notably, in clinical samples, R values distributed samples from healthy donors and cancer patients into unique patterns based on the third marker within specific subclasses. However, across subclasses, R value changes were significantly smaller in healthy samples than in cancer samples. Sorting patterns were simulated using exosomes derived from immune cells, indicating an immune response contribution to the observed signals. These findings highlight the method's potential for identifying biomarkers for specific cancer diagnoses and multi-cancer screening.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100585"},"PeriodicalIF":10.61,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic impedance sensing distinguishes cancer cell states: A step towards point-of-care diagnostics

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-10 DOI: 10.1016/j.biosx.2025.100589

Mahtab Kokabi , Gulam M. Rather , Mehdi Javanmard

{"title":"Microfluidic impedance sensing distinguishes cancer cell states: A step towards point-of-care diagnostics","authors":"Mahtab Kokabi , Gulam M. Rather , Mehdi Javanmard","doi":"10.1016/j.biosx.2025.100589","DOIUrl":"10.1016/j.biosx.2025.100589","url":null,"abstract":"<div><div>In this study, we employed a custom microfluidic sensor to measure the electrical impedance of cancer cells, aiming to differentiate between suspension and adherent phenotypes. We investigated three suspension cell lines—JeKo-1, MM-1R, and Maver-1—representing hematological malignancies, and two adherent breast cancer cell lines—MDA-MB-231 and MDA- MB-468. The impedance measurements revealed significant differences corresponding to the cells’ growth patterns. Suspension cells exhibited lower median impedance values compared to adherent cells, likely due to variations in cell size, complexity, and membrane properties. Suspension cells, being smaller and less structurally complex, demonstrated reduced impedance, whereas adherent cells, which are larger and form stronger surface attachments, displayed higher impedance values. These findings highlight the potential of electrical impedance as a tool for distinguishing cancer cell types. To further validate these results, we propose focusing on the transition of a single cancer cell type between adherent and suspension states, simulating in vitro cancer proliferation conditions. This approach will provide a deeper understanding of how electrical properties evolve during this transition and may offer insights into distinguishing between benign and metastatic cancers or assessing metastatic stages. The observed reduction in impedance during the adherent-to-suspension transition supports the potential diagnostic utility of this method. Overall, this study demonstrates that electrical impedance provides a rapid, label-free approach for enhancing cancer cell diagnostics based on distinct electrical characteristics.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100589"},"PeriodicalIF":10.61,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating voltammetry and substrate-enhanced luminescence for noninvasive glucose sensing

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-07 DOI: 10.1016/j.biosx.2025.100591

Gabriel López-Peña , Ana Pallarés Vilar , Aida Jaafar , Silvia Simón-Fuente , Antonio Arranz , Maria Ribagorda , Miguel Manso-Silván , Emma Martín Rodríguez

{"title":"Integrating voltammetry and substrate-enhanced luminescence for noninvasive glucose sensing","authors":"Gabriel López-Peña , Ana Pallarés Vilar , Aida Jaafar , Silvia Simón-Fuente , Antonio Arranz , Maria Ribagorda , Miguel Manso-Silván , Emma Martín Rodríguez","doi":"10.1016/j.biosx.2025.100591","DOIUrl":"10.1016/j.biosx.2025.100591","url":null,"abstract":"<div><div>This study presents a novel noninvasive and enzyme-free dual sensor based on porous silicon (PSi) for the detection of D-glucose through tear fluid analysis. The sensor incorporates NaGdF<sub>4</sub>:20% Yb<sup>3+</sup>, 2% Er<sup>3+</sup> nanoparticles (YbEr-NPs) immobilized on a PSi substrate functionalized with a phenylboronic ester molecule. The YbEr-NPs enable luminescence-based sensing, exploiting the sensitivity of Er<sup>3+</sup> ions to the OH vibrational groups present in D-glucose. Electrodes fabricated on a PSi substrate allow for voltammetric analysis, providing a second detection method. The voltammetric sensor achieved a limit of detection (LoD) of 20 mg/dL in the range of 20–200 mg/dL, which is suitable for detecting D-glucose concentrations in the tear fluid of diabetic patients. The luminescence-based sensor, utilizing the red-to-green emission ratio of YbEr-NPs, reached a LoD of 140 mg/dL in the range of 10–70 mg/dL, covering the hyperglycemic range. The interaction between the YbEr-NPs and the PSi substrate led to the appearance of new emission bands and increased intensity, attributed to surface defects acting as an additional excitation source. The 556 nm emission band showed a strong dependence on the D-glucose concentration, improving the LoD to 110 mg/dL. This provides a novel strategy for D-glucose detection based on the effect of the molecule on the interaction between the Rare-earth-doped nanoparticles and the PSi substrate. This triple-sensing approach offers a promising solution for noninvasive glucose monitoring, enabling detection in both the hypoglycemic and hyperglycemic ranges.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100591"},"PeriodicalIF":10.61,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrolyte-gated transistor for the monitoring of a CRISPR/Cas activity

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-06 DOI: 10.1016/j.biosx.2025.100590

Pierre Guermonprez , Le Tu Anh , Louis Renaud , Pierre Nioche , Eric Krejci , Aurélie Alleaume-Butaux , Nicolas Battaglini , Vu Thi Thu , Sébastien Sanaur , Benoît Piro

{"title":"An electrolyte-gated transistor for the monitoring of a CRISPR/Cas activity","authors":"Pierre Guermonprez , Le Tu Anh , Louis Renaud , Pierre Nioche , Eric Krejci , Aurélie Alleaume-Butaux , Nicolas Battaglini , Vu Thi Thu , Sébastien Sanaur , Benoît Piro","doi":"10.1016/j.biosx.2025.100590","DOIUrl":"10.1016/j.biosx.2025.100590","url":null,"abstract":"<div><div>Detection of Ribonucleic acids (RNA) is a critical step in the identification of viral or bacterial infections in humans and animals. Reverse transcriptase-polymerase chain reaction (RT-PCR) remains the gold standard, but clustered regularly interspaced short palindromic repeats linked to a Cas endoribonuclease particle (CRISPR/Cas) have recently revolutionized the recognition step of two RNAs, i.e. the CRISPR-RNA (crRNA) and the target, providing a much better selectivity compared to the naked hybridization on which RT-PCR is based. Here, we combine the high efficiency of the CRISPR/Cas13a system with the transduction and amplification capabilities of an electrolyte-gated graphene field-effect transistor (EGGFET) for the detection of specific RNA sequences. In these devices, fabricated on flexible plastic substrates, the active material (reduced graphene oxide, rGO) is deposited by printing and then functionalized with Au nanoparticles decorated with polyU RNA reporter strands. In this system, the CRISPR/Cas13a complex acts as a catalyst: in the presence of a specific RNA target sequence, the enzymatic function is activated and the polyU RNA reporter strands are cleaved from the Au nanoparticles, inducing a loss of negative charges on the rGO layer. This phenomenon leads to measurable changes in the transfer curve of the transistors. These sensors were tested for the detection of a SARS-CoV-2 RNA sequence and showed a linear response in the range of 10<sup>−7</sup> - 10<sup>2</sup> ng μL<sup>−1</sup> with an estimated limit of detection of 10 fM. This work is an important milestone in the development of the next generation of point-of-care RNA sensors.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100590"},"PeriodicalIF":10.61,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SpAi: A machine-learning supported experimental workflow for high-throughput spheroid production and analysis

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-01-31 DOI: 10.1016/j.biosx.2025.100588

Nedim Hacıosmanoğlu , Murat Alp Güngen , Eylul Gulsen Yilmaz , Emre Ece , Alphan Uzun , Arda Taşcan , Burak M. Görmüş , Ismail Eş , Fatih Inci

{"title":"SpAi: A machine-learning supported experimental workflow for high-throughput spheroid production and analysis","authors":"Nedim Hacıosmanoğlu , Murat Alp Güngen , Eylul Gulsen Yilmaz , Emre Ece , Alphan Uzun , Arda Taşcan , Burak M. Görmüş , Ismail Eş , Fatih Inci","doi":"10.1016/j.biosx.2025.100588","DOIUrl":"10.1016/j.biosx.2025.100588","url":null,"abstract":"<div><div>Three-dimensional (3D) cell cultures, especially spheroids, provide a physiologically accurate model for cancer research in comparison to conventional two-dimensional (2D) cultures. Nevertheless, the difficulties in producing and analyzing spheroids have impeded their extensive use in high-throughput screening—a critical process for drug discovery. This study presents a simplified process for the effective creation and examination of spheroids using a 3D-printed mold casted polydimethylsiloxane (PDMS) microwells. The utilization of our specially constructed mold facilitated the creation of consistent spheroids, which were subsequently exposed to doxorubicin for the purpose of anticancer medication treatment. We herein improved spheroid analysis by including a convolutional neural network (CNN) model, specifically U-Net, into a graphical user interface (GUI). This integration allows for automated detection and measurement of spheroid size from microscope pictures. The performance of this system surpassed that of conventional image analysis methods in terms of both accuracy and efficiency. The implemented workflow offers a scalable and cost-efficient platform for conducting high-throughput drug screening, which has the potential to enhance the success rates of cancer therapies in clinical trials.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100588"},"PeriodicalIF":10.61,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing cervical cancer diagnostics: A shift from traditional techniques to biosensors

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-01-31 DOI: 10.1016/j.biosx.2025.100587

Ubaid Mushtaq Naikoo , Roberto Pilloton , Humaira Farooqi , Jagriti Narang

{"title":"Revolutionizing cervical cancer diagnostics: A shift from traditional techniques to biosensors","authors":"Ubaid Mushtaq Naikoo , Roberto Pilloton , Humaira Farooqi , Jagriti Narang","doi":"10.1016/j.biosx.2025.100587","DOIUrl":"10.1016/j.biosx.2025.100587","url":null,"abstract":"<div><div>Cervical cancer is the most important contributor to ailment and death among females universally, highlighting the urgent need for effective diagnostic tools. Traditional diagnostic techniques, such as Pap smears and HPV testing, though effective, suffer from limitations including invasiveness, variable sensitivity, and dependence on clinical infrastructure. This review explores the transformative potential of biosensors as a non-invasive, cost-effective, and sensitive alternative for cervical cancer diagnostics. Comprehensive analysis of current biosensor technologies, emphasizing their mechanisms, advantages, and potential to overcome the drawbacks of conventional methods. Furthermore, the review discusses recent advancements in biosensor design, including integrating nanomaterials and point-of-care systems, which enhance their diagnostic accuracy and accessibility. Key challenges in the clinical translation of biosensors are also addressed, along with potential solutions and future research directions. By highlighting the shift from traditional techniques to biosensors, this review underscores the promise of these innovative tools in improving early detection and patient outcomes in cervical cancer care.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100587"},"PeriodicalIF":10.61,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143259670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-01-29 DOI: 10.1016/j.biosx.2025.100586

Talia Tene , Diana Coello-Fiallos , Myrian Borja , Narcisa Sánchez , Fabián Londo , Cristian Vacacela Gomez , Stefano Bellucci

引用次数: 0

Fluorescent probes to track complex membrane blebbing

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-01-27 DOI: 10.1016/j.biosx.2025.100584

Juan L. Cortes-Muñoz , Johan-Moritz Kux , Pablo J. Sáez , Arturo Jiménez-Sánchez

{"title":"Fluorescent probes to track complex membrane blebbing","authors":"Juan L. Cortes-Muñoz , Johan-Moritz Kux , Pablo J. Sáez , Arturo Jiménez-Sánchez","doi":"10.1016/j.biosx.2025.100584","DOIUrl":"10.1016/j.biosx.2025.100584","url":null,"abstract":"<div><div>Cellular blebbing, pivotal in processes such as apoptosis, cytokinesis, and migration, involves dynamic interactions between the actomyosin network and microtubules. However, existing probes inadequately capture these simultaneous interactions, limiting our ability to study blebbing mechanisms. We present <strong>AztecBleb</strong> probes (<strong>AztecBleb</strong><sup><strong>DAPI</strong></sup>, <strong>AztecBleb</strong><sup><strong>GFP</strong></sup>, <strong>AztecBleb</strong><sup><strong>TxR</strong></sup>, <strong>AztecBleb</strong><sup><strong>Cy5</strong></sup>), novel fluorescent reporters designed to selectively target and monitor blebbing in real-time. These probes incorporate a pregnenolone-based scaffold as a hydrophobic core derived from abiraterone acetate, facilitating precise localization to blebs and microtubules without disrupting cellular function. Through persistent staining of cell blebs, these photostable and biocompatible probes enable continuous monitoring of blebbing and microtubule dynamics during cellular migration. Our approach provides new insights into the coordination of bleb formation and cytoskeletal remodeling, offering a unique tool for studying motility-driven cellular behaviors.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100584"},"PeriodicalIF":10.61,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0