Biosensors and Bioelectronics: X最新文献

{"title":"SpAi: A machine-learning supported experimental workflow for high-throughput spheroid production and analysis","authors":"Nedim Hacıosmanoğlu ,&nbsp;Murat Alp Güngen ,&nbsp;Eylul Gulsen Yilmaz ,&nbsp;Emre Ece ,&nbsp;Alphan Uzun ,&nbsp;Arda Taşcan ,&nbsp;Burak M. Görmüş ,&nbsp;Ismail Eş ,&nbsp;Fatih Inci","doi":"10.1016/j.biosx.2025.100588","DOIUrl":"10.1016/j.biosx.2025.100588","url":null,"abstract":"<div><div>Three-dimensional (3D) cell cultures, especially spheroids, provide a physiologically accurate model for cancer research in comparison to conventional two-dimensional (2D) cultures. Nevertheless, the difficulties in producing and analyzing spheroids have impeded their extensive use in high-throughput screening—a critical process for drug discovery. This study presents a simplified process for the effective creation and examination of spheroids using a 3D-printed mold casted polydimethylsiloxane (PDMS) microwells. The utilization of our specially constructed mold facilitated the creation of consistent spheroids, which were subsequently exposed to doxorubicin for the purpose of anticancer medication treatment. We herein improved spheroid analysis by including a convolutional neural network (CNN) model, specifically U-Net, into a graphical user interface (GUI). This integration allows for automated detection and measurement of spheroid size from microscope pictures. The performance of this system surpassed that of conventional image analysis methods in terms of both accuracy and efficiency. The implemented workflow offers a scalable and cost-efficient platform for conducting high-throughput drug screening, which has the potential to enhance the success rates of cancer therapies in clinical trials.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100588"},"PeriodicalIF":10.61,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing cervical cancer diagnostics: A shift from traditional techniques to biosensors","authors":"Ubaid Mushtaq Naikoo ,&nbsp;Roberto Pilloton ,&nbsp;Humaira Farooqi ,&nbsp;Jagriti Narang","doi":"10.1016/j.biosx.2025.100587","DOIUrl":"10.1016/j.biosx.2025.100587","url":null,"abstract":"<div><div>Cervical cancer is the most important contributor to ailment and death among females universally, highlighting the urgent need for effective diagnostic tools. Traditional diagnostic techniques, such as Pap smears and HPV testing, though effective, suffer from limitations including invasiveness, variable sensitivity, and dependence on clinical infrastructure. This review explores the transformative potential of biosensors as a non-invasive, cost-effective, and sensitive alternative for cervical cancer diagnostics. Comprehensive analysis of current biosensor technologies, emphasizing their mechanisms, advantages, and potential to overcome the drawbacks of conventional methods. Furthermore, the review discusses recent advancements in biosensor design, including integrating nanomaterials and point-of-care systems, which enhance their diagnostic accuracy and accessibility. Key challenges in the clinical translation of biosensors are also addressed, along with potential solutions and future research directions. By highlighting the shift from traditional techniques to biosensors, this review underscores the promise of these innovative tools in improving early detection and patient outcomes in cervical cancer care.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100587"},"PeriodicalIF":10.61,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143259670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene","authors":"Talia Tene ,&nbsp;Diana Coello-Fiallos ,&nbsp;Myrian Borja ,&nbsp;Narcisa Sánchez ,&nbsp;Fabián Londo ,&nbsp;Cristian Vacacela Gomez ,&nbsp;Stefano Bellucci","doi":"10.1016/j.biosx.2025.100586","DOIUrl":"10.1016/j.biosx.2025.100586","url":null,"abstract":"<div><div>We present a systematic optimization and analysis of SPR biosensors, focusing on the influence of design parameters such as silver (Ag), silicon nitride (Si₃N₄), graphene, and ssDNA layer thicknesses. Two configurations, Sys₃ and Sys₅, were developed and numerically evaluated under varying SARS-CoV-2 concentrations in PBS solution, ranging from 0.01 nM to 100 nM. Sys₃, optimized with Ag at 55 nm, Si₃N₄ at 13 nm, and ssDNA at 10 nm, demonstrated exceptional sensitivity (371.7°/RIU), low limit of detection, and high detection accuracy, making it suitable for precision applications. In contrast, Sys₅, incorporating a graphene layer (0.34 nm) alongside Ag at 50 nm, Si₃N₄ at 10 nm, and ssDNA at 10 nm, exhibited superior robustness and a higher figure of merit (2287.2 RIU⁻¹), offering consistent performance across a broader dynamic range. These results highlight the versatility of SPR biosensors in adapting to diverse diagnostic needs.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100586"},"PeriodicalIF":10.61,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent probes to track complex membrane blebbing","authors":"Juan L. Cortes-Muñoz ,&nbsp;Johan-Moritz Kux ,&nbsp;Pablo J. Sáez ,&nbsp;Arturo Jiménez-Sánchez","doi":"10.1016/j.biosx.2025.100584","DOIUrl":"10.1016/j.biosx.2025.100584","url":null,"abstract":"<div><div>Cellular blebbing, pivotal in processes such as apoptosis, cytokinesis, and migration, involves dynamic interactions between the actomyosin network and microtubules. However, existing probes inadequately capture these simultaneous interactions, limiting our ability to study blebbing mechanisms. We present <strong>AztecBleb</strong> probes (<strong>AztecBleb</strong>^{<strong>DAPI</strong>}, <strong>AztecBleb</strong>^{<strong>GFP</strong>}, <strong>AztecBleb</strong>^{<strong>TxR</strong>}, <strong>AztecBleb</strong>^{<strong>Cy5</strong>}), novel fluorescent reporters designed to selectively target and monitor blebbing in real-time. These probes incorporate a pregnenolone-based scaffold as a hydrophobic core derived from abiraterone acetate, facilitating precise localization to blebs and microtubules without disrupting cellular function. Through persistent staining of cell blebs, these photostable and biocompatible probes enable continuous monitoring of blebbing and microtubule dynamics during cellular migration. Our approach provides new insights into the coordination of bleb formation and cytoskeletal remodeling, offering a unique tool for studying motility-driven cellular behaviors.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100584"},"PeriodicalIF":10.61,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced glucose sensing via nanoparticles-modified extended gates: A novel approach to electric double layer modulation and signal amplification in field effect transistors for improved detection sensitivity","authors":"Sheng-Chun Hung,&nbsp;Yi-Hua Lee","doi":"10.1016/j.biosx.2025.100576","DOIUrl":"10.1016/j.biosx.2025.100576","url":null,"abstract":"<div><div>This study presents an innovative glucose sensing platform that harnesses the enhanced electrocatalytic properties of planar electric double layer (EDL) structures in conjunction with extended gate field effect transistors (EGFETs). By integrating specific nanoparticles onto the sensor surface, this platform achieves substantial improvements in glucose detection sensitivity and overall performance. The materials employed in this research include Ni nanowires combined with graphene films, CuO nanoparticles incorporated into carbon nanostructures, and gold nanoparticles affixed to ZnO nanostructures. These nanomaterials exhibit remarkable catalytic activity, while the localized electric field effect generated by the electric double layer significantly amplifies the signal, thereby enhancing sensitivity. Experimental findings reveal notable enhancements in both sensitivity and detection limits compared to conventional glucose sensors, underscoring the potential of this platform for effective glucose monitoring. Specifically, the Ni nanowire-graphene film sensor recorded a sensitivity of 3102.7 μA mM⁻¹ cm⁻² with a detection limit of 51 nM. The CuO nanoparticle-carbon nanostructure sensor achieved a sensitivity of 2206.25 μA mM⁻¹ cm⁻² and a detection limit of 39 nM, while the gold nanoparticle-ZnO nanostructure sensor demonstrated a sensitivity of 811.8 μA mM⁻¹ cm⁻² with a detection limit of 59 nM.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100576"},"PeriodicalIF":10.61,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of Interferometric Reflectance Imaging Sensor as a sensitive detection platform and its application areas","authors":"Monireh Bakhshpour-Yucel ,&nbsp;Nese Lortlar Unlu ,&nbsp;Elif Seymour ,&nbsp;Adil Denizli","doi":"10.1016/j.biosx.2025.100574","DOIUrl":"10.1016/j.biosx.2025.100574","url":null,"abstract":"<div><div>The Interferometric Reflectance Imaging Sensor (IRIS) technology represents a significant advancement in biosensing, providing a label-free, selective, sensitive, and high-throughput platform for detecting molecular interactions. This review explores the underlying principles, instrumentation, and diverse applications of IRIS, with a focus on its efficacy for real-time monitoring of DNA-protein and protein-protein interactions, as well as virus detection. IRIS can measure DNA hybridization kinetics and identify pathogens without labeling, highlighting its versatility and reliability in biomedical research and diagnostics. IRIS achieves enhanced sensitivity and specificity by leveraging spectral reflectivity as a transduction mechanism and employing a 3D polymeric surface chemistry for bioreceptor immobilization. The review underscores IRIS's potential to revolutionize clinical diagnostics, biomolecular screening, and the study of biomolecular binding affinities, establishing it as a powerful tool for future research and medical applications.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"22 ","pages":"Article 100574"},"PeriodicalIF":10.61,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Personal glucose meter-based portable method for creatine kinase assay","authors":"Jinhwan Lee ,&nbsp;Yejin Oh ,&nbsp;Hyoyong Kim,&nbsp;Sangmo Lee,&nbsp;Hyun Gyu Park","doi":"10.1016/j.biosx.2025.100575","DOIUrl":"10.1016/j.biosx.2025.100575","url":null,"abstract":"<div><div>We herein developed a simple and label-free method to detect the creatine kinase (CK) activity by employing a personal glucose meter (PGM) as a detection device. This method relies on the target-induced consumption of glucose by the cascade enzymatic reaction (CER) promoted by the combined activities of CK and hexokinase (HK). The target CK first catalyzes the phosphorylation of adenosine 5′-diphosphate (ADP) to adenosine 5′-triphosphate (ATP) using creatine phosphate (CP) as a phosphate donor. HK then phosphorylates glucose into glucose-6-phosphate by converting the produced ATP back to ADP, which could enter another cycle of the CER to further phosphorylate glucose in a sample. As a consequence, the glucose level would decrease depending on the CK activity, which could be very conveniently detected using a PGM. With this unique design principle, we successfully determined the CK activity down to 0.0147 U/mL with high specificity against various non-target enzymes. We also verified that the developed strategy can reliably detect CK activity in heterogeneous human blood, ensuring its robust utility in various clinical applications. This user-friendly method enables point-of care detection of CK, offering a practical tool for monitoring muscle health. By allowing simultaneous tracking of CK and glucose levels with the same PGM device, this approach is particularly beneficial for diabetes mellitus (DM) patients, empowering them to proactively manage their health and reduce the risk of muscle-related complications.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"22 ","pages":"Article 100575"},"PeriodicalIF":10.61,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fiber-tip photonic crystal for real-time referenced biosensing in serum","authors":"Mathias Dolci ,&nbsp;Paco Dreverman ,&nbsp;Mildred S. Cano-Velázquez ,&nbsp;Arthur L. Hendriks ,&nbsp;Emiel Veth ,&nbsp;P.J. van Veldhoven ,&nbsp;Andrea Fiore ,&nbsp;Peter Zijlstra","doi":"10.1016/j.biosx.2024.100573","DOIUrl":"10.1016/j.biosx.2024.100573","url":null,"abstract":"<div><div>Fiber optic sensors have become increasingly well-established due to the many advantages they provide such as immunity to electromagnetic interference, multiplexing capabilities, and remote sensing. The coupling of light with a transducer at the tip of the optical fiber enables the detection of physical and biological parameters. 2D photonic crystals (PhC) can be designed to feature guided-mode resonances (GMR) characterized by a strong electric field at the PhC surface, providing a suitable tool for the detection of local refractive index variations (e.g. biomolecule adsorption). Here, we demonstrate the use of a PhC transferred to the tip of a single-mode fiber for biosensing. The control of surface chemistry provides a sensitive platform for the molecular recognition of antibody biomarkers. By integrating the fiber in a continuous flow platform, the real-time detection of anti-IgG in undiluted serum was achieved, with a limit of detection down to 60 pM. Moreover, the use of a reference channel is demonstrated to correct for signal drifts in real-time due to changes in bulk refractive index. These referenced fiber-tip PhC biosensors may pave the way for fluidic integrated systems in environmental, industrial, and healthcare applications, and open up the possibility of biosensing in the human body by integrating them into catheters.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"22 ","pages":"Article 100573"},"PeriodicalIF":10.61,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model","authors":"Zaid H. Mahmoud ,&nbsp;Uday Abdul-Reda Hussein ,&nbsp;Najwa Aljbory ,&nbsp;Mohammed Jawad Alnajar ,&nbsp;Laleh Maleknia ,&nbsp;Abolfazl Mirani ,&nbsp;Ehsan kianfar","doi":"10.1016/j.biosx.2024.100570","DOIUrl":"10.1016/j.biosx.2024.100570","url":null,"abstract":"<div><h3>Background and objectives</h3><div>Nanofibers have a high specific surface area and small pores, which increases the possibility of drug uptake. In this study, chasteberry-containing nanofibers were prepared by electrospinning to investigate their potential for polycystic follicles after induction of polycystic ovarian syndrome (PCOS).</div></div><div><h3>Materials and methods</h3><div>Chasteberry-containing nanofibers were identified using Fourier-transform infrared spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM), and thermal gravimetric analysis (TGA) tests, as well as drug release and antibacterial tests. In this study, 42 Sprague-Dawley rats with regular menstrual cycles were divided into five groups: control group (healthy rats), polycarbonate (PC) group (treated with 1 mg/kg letrozole for 28 days to induce PCOS syndrome), and Poly terephthalate (PT) group (treated with PCOS for 30 days). Chasteberry was included at 1, 2, 4 wt%. To induce PCOS, the polyterephthalate (PT) and polycarbonate (PC) groups were administered 1 mg/kg letrozole by oral gavage for 28 days. The polyterephthalate (PT) group was treated with different doses of chasteberry-containing nanofibers (1, 2, 4 wt%).</div></div><div><h3>Results</h3><div>The results showed that the drug was well incorporated into the fibers and released slowly and sustainedly from the nanofibers for 30 days. Ex vivo results showed that by taking letrozole, the polycarbonate (PC) group had larger cystic follicles with a very thin granulosa layer than the control group. This is the cause of ovarian anovulation or oligo-ovulation and induction of polycystic ovarian syndrome. In the polyterephthalate (PT) group, which was administered nanofibers containing chasteberry nanomedicine subcutaneously, the number of cystic follicles decreased and the number of various follicles indicating ovulation increased in these groups.</div></div><div><h3>Conclusion</h3><div>Subcutaneous administration of Vitex nanofibers at a dose of 4 wt% can improve polycystic ovarian syndrome by decreasing cystic follicles and increasing the number of various follicles and corpora lutea.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"22 ","pages":"Article 100570"},"PeriodicalIF":10.61,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous detection of human neutrophil elastase and cathepsin G on a single substrate using a fluorometric quantum dots probe and chemometric models","authors":"Fátima A.R. Mota,&nbsp;Rafael C. Castro,&nbsp;David S.M. Ribeiro,&nbsp;João L.M. Santos,&nbsp;Ricardo N.M.J. Páscoa,&nbsp;Marieta L.C. Passos,&nbsp;M. Lúcia M.F.S. Saraiva","doi":"10.1016/j.biosx.2024.100571","DOIUrl":"10.1016/j.biosx.2024.100571","url":null,"abstract":"<div><div>Human neutrophil elastase (HNE) and cathepsin G (CatG) are crucial proteolytic enzymes involved in the pathophysiology of chronic wounds. High levels of these enzymes indicate prolonged inflammation and impaired healing processes, making their discrimination and quantification essential for effective wound management and treatment strategies. In this study, we propose a novel method combining distinctly sized CdTe quantum dots (QDs) as a fluorescent probe to implement a platform for simultaneous discrimination and quantification of HNE and CatG, applying chemometric analysis.</div><div>The fluorometric response was acquired using two different methods: kinetic and excitation/emission matrices (EEM). These second-order data were processed using various chemometric models, including unfolded partial least-squares with residual bilinearization (U-PLS/RBL), radial basis function artificial neural network (RBF-ANN), and partial least squares-discriminant analysis (PLS-DA), to guarantee a detailed and precise analysis. The results showed that the kinetic method, when processed with the aforementioned models, accurately quantified CatG in the presence of HNE with a REP of around 20%. This method also successfully discriminated the two enzymes both together and individually, achieving a sensitivity and specificity of 1. In contrast, the EEM method only allowed for the discrimination of the two enzymes both together and individually.</div><div>Our groundbreaking approach proved to be accurate for both the discrimination and quantification of one of the enzymes, offering the advantage of being simpler and faster than other reference procedures. This method paves the way for more effective therapeutic interventions and could initiate a path toward the simultaneous discrimination of multiple enzymes.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"22 ","pages":"Article 100571"},"PeriodicalIF":10.61,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}