Biosensors and Bioelectronics: X最新文献

{"title":"Recent advances in wearable electrochemical biosensors towards technological and material aspects","authors":"Mahan Hosseinzadeh Fakhr ,&nbsp;Ivan Lopez Carrasco ,&nbsp;Dmitry Belyaev ,&nbsp;Jihun Kang ,&nbsp;YeHyeon Shin ,&nbsp;Jong-Souk Yeo ,&nbsp;Won-Gun Koh ,&nbsp;Jeongwoo Ham ,&nbsp;Alexander Michaelis ,&nbsp;Joerg Opitz ,&nbsp;Natalia Beshchasna","doi":"10.1016/j.biosx.2024.100503","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100503","url":null,"abstract":"<div><p>The next generation of wearable biosensors comes with the latest advancements in biosensor technology. Soft and stretchable electrode materials like hydrogels with the similar functionalities of human tissue including stretchability, self-healability, and responsiveness to different stimuli have emerged as the most versatile materials in wearable electronics. The incorporation of conductive nanofillers is found to enhance the sensitivity of the electrochemical biosensors significantly. Microfluidic technology has reduced the volume of samples and reagents required for the analysis, allowing continuous biomedical monitoring from a drop of biofluid. In this paper, the most advanced progress in electrochemical wearable platforms that can noninvasively and continuously monitor the biochemical markers in body fluids for the diagnosis and health management is reviewed. Innovation in microelectronics, modification, fabrication technologies, and detection methods are the main focus of the discussion. In particular, hydrogel-based sensors and microfluidic systems as the latest technology trends in wearable detection are discussed in detail. Integration of miniaturized electrochemical wearable biosensors with wireless technology as a great promise for real-time healthcare monitoring and point-of-care (POC) diagnostics is also summarized. Finally, we outline the most advanced wearable biosensors with optimized material and design as well as key challenges that need to be addressed to improve sensing performance (accuracy, sensitivity, selectivity, stability), portability (miniaturized size and light weight), and flexibility of the wearable biosensors.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100503"},"PeriodicalIF":10.61,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000670/pdfft?md5=884e47b94f329229f5631b2585fe9d41&pid=1-s2.0-S2590137024000670-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141249515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of microchip electrophoresis pulsed amperometric detector-based soil pesticide analyser","authors":"Rishi Raj ,&nbsp;Suddhasatwa Basu ,&nbsp;Sandeep Kumar Jha","doi":"10.1016/j.biosx.2024.100502","DOIUrl":"10.1016/j.biosx.2024.100502","url":null,"abstract":"<div><p>The present study involved the fabrication and testing of a Microchip electrophoresis (MCE) device for pulse amperometry based detection of pesticides from their mixture. We were able to separate and then quantify three distinct types of insecticides, namely Chlorpyrifos, Imidacloprid, and Fipronil using on chip MCE followed by pulsed amperometric detection. All these results were obtained with an inhouse developed potentiostat cum controller unit with a detection time of only 15 min, employing a minimal sample size of 2 μL without any preconcentration or extraction procedure. The limit of detection (LOD) was calculated as 42.69 μM, 62.61 μM, and 71.14 μM or 14.96, 16.0 and 31.09 ppm, respectively for Chlorpyrifos, Imidacloprid, and Fipronil and their respective migration times as 536 ± 6.3 s, 484 ± 1.7 s, and 604 ± 3.5 s (n = 14). The sensitivity of detection was determined as 0.03 nA/μM for Chlorpyrifos, 0.0265 nA/μM for Imidacloprid, and 0.035 nA/μM for Fipronil. In addition, the efficacy of the produced microchip was confirmed by analysing soil extract spiked with known pesticides concentrations while the recovery percentage, representing a ratio of calculated concentration to spiked concentration multiplied by hundred was found as 84.3% (±9.4%) (n = 9). Thus, integrating microchip technology with the developed analytical instruments presents significant promise for practical field applications and the analysis of diverse analytes by way of creating a library where the migration coefficient and peak detection current are needed for any analyte which can be made cationic or anionic using a suitable buffer.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100502"},"PeriodicalIF":10.61,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000669/pdfft?md5=c3b3cbcebf197ef8233415b537d5141d&pid=1-s2.0-S2590137024000669-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141194588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable technology for one health: Charting the course of dermal biosensing","authors":"Georgeta Vulpe ,&nbsp;Guoyi Liu ,&nbsp;Sam Oakley ,&nbsp;Dimitrios Pletsas ,&nbsp;Guanghao Yang ,&nbsp;Rosa Dutra ,&nbsp;Owen Guy ,&nbsp;Yufei Liu ,&nbsp;Mark Waldron ,&nbsp;Joe Neary ,&nbsp;Arjun Ajith Mohan ,&nbsp;Sanjiv Sharma","doi":"10.1016/j.biosx.2024.100500","DOIUrl":"10.1016/j.biosx.2024.100500","url":null,"abstract":"<div><p>Over the last decade, a significant paradigm shift has been observed towards leveraging less invasive biological fluids—such as skin interstitial fluid (ISF), sweat, tears, and saliva—for health monitoring. This evolution seeks to transcend traditional, invasive blood-based methods, offering a more accessible approach to health monitoring for non-specialized personnel. Skin ISF, with its profound resemblance to blood, emerges as a pivotal medium for the real-time, minimally invasive tracking of a broad spectrum of biomarkers, thus becoming an invaluable asset for correlating with blood-based data. Our exploration delves deeply into the development of wearable molecular biosensors, spotlighting dermal sensors for their pivotal roles across both clinical and everyday health monitoring scenarios and underscoring their contributions to the holistic One Health initiative. In bringing forward the myriad challenges that permeate this field, we also project future directions, notably the potential of skin ISF as a promising candidate for continuous health tracking.</p><p>Moreover, this paper aims to catalyse further exploration and innovation by presenting a curated selection of seminal technological advancements. Amidst the saturated landscape of analytical literature on translational challenges, our approach distinctly seeks to highlight recent developments. In attracting a wider spectrum of research groups to this versatile domain, we endeavour to broaden the collective understanding of its trajectory and potential, mapping the evolution of wearable biosensor technology. This strategy not only illuminates the transformative impact of wearable biosensors in reshaping health diagnostics and personalized medicine but also fosters increased participation and progress within the field. Distinct from recent manuscripts in this domain, our review serves as a distillation of key concepts, elucidating pivotal papers that mark the latest advancements in wearable sensors. Through presenting a curated collection of landmark studies and offering our perspectives on the challenges and forward paths, this paper seeks to guide new entrants in the area. We delineate a division between wearable epidermal and subdermal sensors—focusing on the latter as the future frontier—thereby establishing a unique discourse within the ongoing narrative on wearable sensing technologies.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100500"},"PeriodicalIF":10.61,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000645/pdfft?md5=e560ae9f111b111632d58b48d0376254&pid=1-s2.0-S2590137024000645-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141130332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards monitoring of critical illness via the detection of histones with extended gate field-effect transistor sensors","authors":"Hayley Richardson ,&nbsp;Jeffrey Barahona ,&nbsp;Greg Medwig ,&nbsp;Angela Johns ,&nbsp;Lina M. Acosta Pérez ,&nbsp;Koji Sode ,&nbsp;Michael Daniele ,&nbsp;Francis J. Miller ,&nbsp;Edgar Lobaton ,&nbsp;Spyridon Pavlidis","doi":"10.1016/j.biosx.2024.100501","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100501","url":null,"abstract":"<div><p>Extracellular histone proteins in the blood indicate a heightened risk of morbidity after trauma or in major illnesses such as sepsis. We present the development of an aptasensor for histone detection with an extended gate field-effect transistor (EGFET) configuration, which benefits from low power consumption, rapid response, and compatibility with miniaturized gold electrodes. Histones have a high isoelectric point and charge density, which cause them to physically adsorb to non-specific elements of the sensor that have available electrostatic charges. To combat this, the sensing surface is formed with a thiol-modified, high-affinity and histone-specific RNA aptamer sequence and by co-immobilizing with poly(ethylene glycol) methyl ether thiol (PEG) as a blocking agent. Surface plasmon resonance (SPR) is used to analyze aptamer and PEG immobilization strategies, confirm histone binding, and calculate kinetic binding constants. Through comparison of different blocking agents and time-dependent preparation, the ideal equilibrium dissociation constant (K_D) is estimated to be below 200 pM, which is the upper range of extracellular histone concentrations in critically ill patients with high mortality. The EGFET sensitivity of the optimized aptasensor is 6.65 mV/decade concentration change for histone H4 with a physiologically relevant 5 pM limit of detection. Selectivity tests with 100 nM bovine serum albumin (BSA) demonstrate a signal response that is 13-fold smaller than for histones. This EGFET aptasensor platform is suitable for future point-of-care monitoring of histone levels in critically ill patients, thus permitting the early detection of increased risk and the need for more aggressive interventional measures to prevent mortality.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100501"},"PeriodicalIF":10.61,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000657/pdfft?md5=5abf3262c26bbda68acae06aba70ee43&pid=1-s2.0-S2590137024000657-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141095073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advancements in microfluidic-based biosensors for detection of genes and proteins: Applications and techniques","authors":"Saeed Siavashy ,&nbsp;M. Soltani ,&nbsp;Shayan Rahimi ,&nbsp;Mehraveh Hosseinali ,&nbsp;Zahra Guilandokht ,&nbsp;Kaamran Raahemifar","doi":"10.1016/j.biosx.2024.100489","DOIUrl":"10.1016/j.biosx.2024.100489","url":null,"abstract":"<div><p>This article has tried to provide an overview of the most sophisticated microfluidic biosensors for identifying nucleic acids and proteins at the site of treatment. Microfluidics, which also automates sample preparation and reduces processing time and reagent consumption, enables the analysis of small sample quantities. Microfluidics and biosensor technologies collaborate to provide diagnostics at the point of care with high throughput analysis, portability, and disposability. The high sensitivity and selectivity requirements, false response errors, and integration with other essential modules are some of the challenges posed by this merger. The broad categories of protein-based and DNA-based biosensor technology are covered in this review. Also, recent advancements in coupling the biosensors to microfluidics, the main challenges and potential solutions in deploying microfluidic biosensors for point-of-care diagnostics, and the most recent developments in these areas have been discussed.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100489"},"PeriodicalIF":10.61,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000530/pdfft?md5=4b74ac1abe95839b91f7b5b1622ed236&pid=1-s2.0-S2590137024000530-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141140676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A digital microfluidic device integrated with electrochemical sensor and 3D matrix for detecting soluble PD-L1","authors":"Yuqian Zhang ,&nbsp;Jing Liu ,&nbsp;Ting-Wen Lo ,&nbsp;Yohan Kim ,&nbsp;Fabrice Lucien ,&nbsp;Haidong Dong ,&nbsp;Yuguang Liu","doi":"10.1016/j.biosx.2024.100490","DOIUrl":"10.1016/j.biosx.2024.100490","url":null,"abstract":"<div><p>PD1/PD-L1 checkpoint inhibitors are at the forefront of cancer immunotherapies. However, the overall response rate remains only 10–30%. Even among initial responders, drug resistance often occurs, which can lead to prolonged use of a futile therapy in the race with the fatal disease. It would be ideal to closely monitor key indicators of patients’ immune responsiveness, such as circulating PD-L1 levels. Traditional PD-L1 detection methods, such as ELISA, are limited in sensitivity and rely on core lab facilities, preventing their use for the regular monitoring. Electrochemical sensors exist as an attractive candidate for point-of-care tool, yet, streamlining multiple processes in a single platform remains a challenge. To overcome this challenge, this work integrated electrochemical sensor arrays into a digital microfluidic device to combine their distinct merits, so that soluble PD-L1 (sPD-L1) molecules can be rapidly detected in a programmed and automated manner. This new platform featured microscale electrochemical sensor arrays modified with electrically conductive 3D matrix, and can detect as low as 1 pg/mL sPD-L1 with high specificity. The sensors also have desired repeatability and can obtain reproducible results on different days. To demonstrate the functionality of the device to process more complex biofluids, we used the device to detect sPD-L1 molecules secreted by human breast cancer cell line in culture media directly and observed 2X increase in signal compared with control experiment. This novel platform holds promise for the close monitoring of sPD-L1 level in human physiological fluids to evaluate the efficacy of PD-1/PD-L1 immunotherapy.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100490"},"PeriodicalIF":10.61,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000542/pdfft?md5=1454dd20b58fa125621fb21bce0827cc&pid=1-s2.0-S2590137024000542-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141026876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel aptamer-antibody sandwich electrochemical sensor for detecting ADAR1 in complex biological samples","authors":"Madhu Biyani ,&nbsp;Kirti Sharma ,&nbsp;Maeda Shoei ,&nbsp;Hinako Akashi ,&nbsp;Masataka Nakano ,&nbsp;Miki Nakajima ,&nbsp;Manish Biyani","doi":"10.1016/j.biosx.2024.100491","DOIUrl":"10.1016/j.biosx.2024.100491","url":null,"abstract":"<div><p>Human adenosine deaminase acting on RNA1 (ADAR1) is an adenosine-to-inosine (A-to-I) RNA-editing enzyme involved in various types of cancer progression. ADAR1 has emerged as a novel prognostic biomarker for cancer. This study describes the application of a newly identified 70-nt DNA aptamer (Apt38483) against ADAR1 to develop a portable and simple electrochemical biosensor platform for the rapid and sensitive detection of ADAR1 in cell lysates. We selected an ADAR1-specific DNA aptamer from a randomized 70-nt single-stranded DNA library using a competitive in vitro selection method. ADAR1 in the cell lysate was sandwiched onto a bare carbon working electrode of an electro-chemically printed chip between the ADAR1 antibody and gold nanoparticles (40 nm) conjugated with Apt38483, followed by electrochemical analysis using differential pulse voltammetry (DPV) for sensor demonstration. A highly sensitive change in current was observed for as little as 0.53 nM ADAR1 in human embryonic kidney cell lysate. Thus, the merging of a novel DNA aptamer probe for ADAR1 with an electrochemical transduction method enabled the development of a simple, low-cost, and rapid method for the direct measurement of ADAR1 in cell lysates and indicated great potential for the development of an ADAR1 analysis platform, which would be useful in cancer prognosis.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100491"},"PeriodicalIF":10.61,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000554/pdfft?md5=c29da9dcfd202f3d2a83cd3500d6c8a1&pid=1-s2.0-S2590137024000554-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141024284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible cdots dual role: Nanophotosensitizers for hypoxic photodynamic therapy and scavenging nanozyme biosensing","authors":"Helena M.R. Gonçalves ,&nbsp;Susana A.F. Neves ,&nbsp;Sabela Rodriguez ,&nbsp;Rui F.P. Pereira ,&nbsp;Rui Vilarinho ,&nbsp;Tânia Moniz ,&nbsp;Maria Rangel ,&nbsp;J. Agostinho Moreira ,&nbsp;Paula Martins-Lopes ,&nbsp;Filomena Adega ,&nbsp;Frank Davis ,&nbsp;Séamus P.J. Higson ,&nbsp;Marita A. Cardoso","doi":"10.1016/j.biosx.2024.100499","DOIUrl":"10.1016/j.biosx.2024.100499","url":null,"abstract":"<div><p>Within this study we report a non-toxic nanomaterial suitable for Photodynamic Therapy (PDT) under hypoxic conditions. PDT relies on the production of reactive oxygen and nitrogen species that can lead to cancer cells death. Currently, PDT is limited by: the development of efficient photosensitizers that can produce these radicals <em>in situ</em>; and the oxygen level requirement. The produced Carbon Dots (Cdots) successfully destroy human melanoma cancer cells upon 5 min irradiation using 450 nm wavelength due to the <em>in situ</em> production of NO•. As such, this nanophotosensitizer is applicable regardless of the cells molecular oxygen levels. Additionally, this nanomaterial acts as a scavenging nanozyme biosensor allowing to follow-up, <em>in situ,</em> the released NO• concentration, thereby offering a tight control over the NO• concentration in real-time and, its maintenance within the therapeutic window. Hence, this work offers a novel theranostic and NO• scavenging nanozyme biosensing nanoplatform that allows high control for hypoxic-PDT cancer application even in low doses and with 5 min irradiation.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100499"},"PeriodicalIF":10.61,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000633/pdfft?md5=4007d6b399d69040365fd0159ac703ff&pid=1-s2.0-S2590137024000633-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141027128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal-organic frameworks as an active substrate for cell-interaction studies and cell-on-a-chip platforms","authors":"Huijie Jiang ,&nbsp;Ziyu Gao ,&nbsp;Claudia Lubrano ,&nbsp;Claudia Latte Bovio ,&nbsp;Henning Bommes ,&nbsp;Andrea Kauth ,&nbsp;Lea Baumann ,&nbsp;Bo Cheng ,&nbsp;Divagar Murugan ,&nbsp;Joachim Knoch ,&nbsp;Rainer Waser ,&nbsp;Sven Ingebrandt ,&nbsp;Francesca Santoro ,&nbsp;Vivek Pachauri","doi":"10.1016/j.biosx.2024.100487","DOIUrl":"10.1016/j.biosx.2024.100487","url":null,"abstract":"<div><p>Metal-organic frameworks (MOFs) are an emerging class of nanomaterials with immense biomedical potential for their unique interactions with biological and organic materials. In this work, we select two candidate two-dimensional (2D) MOF systems based on Fe³⁺ and Ni²⁺ metal centers and 2-aminoterephthalate acid ligand (Fe-MOF and Ni-MOF) and evaluate their performance as an active interface for study of cell-interactions. 2D Fe-MOF and Ni-MOF were synthesized onto hydroxyl-modified gold and glass substrates using a layer-by-layer liquid-phase-epitaxy (LbL-LPE) growth at room temperature and used as active substrates (Fe-MOF/glass, Fe-MOF/Au, Ni-MOF/glass and Ni-MOF/Au, respectively) for MTT cell-proliferation and reactive oxygen species tests using the PC-12 cell-line in order to investigate the biocompatibility. Immunostaining and morphological analyses of PC-12 cells on MOF interfaces suggested a stronger cell-substrate interaction in comparison to glass and were further characterized using the Electrical Cell-substrate Impedance Sensing (ECIS) technique, here for the first time, employed to study cell attachment, spreading and proliferation on 2D Fe-MOF. The 2D Fe-MOF showed superior long-term stability in cell culture medium by recording impedance over 24 h, crucial to monitor cell-dynamics at a solid-liquid interface. A significant increase of interfacial impedance was observed in ECIS, due to PC-12 cells adhering onto 2D Fe-MOF, which was also confirmed by the focused ion beam etching followed by scanning electron microscopy. Our novel findings, therefore, suggest 2D MOFs as highly suitable platform for the study of cell-related interactions using electrical techniques and potentially pave the way for future use of MOFs for bioelectronics and biosensor applications.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100487"},"PeriodicalIF":10.61,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000517/pdfft?md5=b7c0a4c8aa371afad48b558e2ec46c2b&pid=1-s2.0-S2590137024000517-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141051683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold nanosheet modified electrode with reduced impedance for electrophysiological recordings","authors":"Mohaddeseh Vafaiee ,&nbsp;Farzaneh Aghakhani Mahyari ,&nbsp;Asadollah Kalantarian ,&nbsp;Mahyar Janahmadi ,&nbsp;Raheleh Mohammadpour ,&nbsp;Azam Iraji zad ,&nbsp;Pezhman Sasanpour","doi":"10.1016/j.biosx.2024.100485","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100485","url":null,"abstract":"<div><p>In neurophysiological recording, reducing electrode impedance is crucial for enhancing the signal-to-noise ratio and achieving the desired spatial resolution. This study presents an approach to improve the performance of Au/Cr/glass electrodes by incorporating synthesized gold nanosheets without the need for additional adhesive material. We characterized the performance of the modified electrodes using electrochemical impedance spectroscopy and equivalent circuit analysis. Our findings showed an 81% reduction in mean impedance for the modified electrode, which was 0.85 kΩ at 1 kHz, compared to the unmodified electrode at 4.5 kΩ, an improvement attributed to the higher effective surface area of the modified electrode. Additionally, Scanning electron microscopy observations of PC12 cells cultured on the modified electrodes indicated favorable cell elongation and interaction with the rough surface. Stability studies indicated acceptable performance of the modified electrodes in solution environments. These results suggest that surface modification of electrodes with gold nanosheets could be a promising strategy for enhancing neural interface applications.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"18 ","pages":"Article 100485"},"PeriodicalIF":10.61,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000499/pdfft?md5=a940cac3b03c7809d30aa8563bf846cd&pid=1-s2.0-S2590137024000499-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}