Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X Pub Date : 2025-01-29 DOI:10.1016/j.biosx.2025.100586

Talia Tene , Diana Coello-Fiallos , Myrian Borja , Narcisa Sánchez , Fabián Londo , Cristian Vacacela Gomez , Stefano Bellucci

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引用次数: 0

Abstract

We present a systematic optimization and analysis of SPR biosensors, focusing on the influence of design parameters such as silver (Ag), silicon nitride (Si₃N₄), graphene, and ssDNA layer thicknesses. Two configurations, Sys₃ and Sys₅, were developed and numerically evaluated under varying SARS-CoV-2 concentrations in PBS solution, ranging from 0.01 nM to 100 nM. Sys₃, optimized with Ag at 55 nm, Si₃N₄ at 13 nm, and ssDNA at 10 nm, demonstrated exceptional sensitivity (371.7°/RIU), low limit of detection, and high detection accuracy, making it suitable for precision applications. In contrast, Sys₅, incorporating a graphene layer (0.34 nm) alongside Ag at 50 nm, Si₃N₄ at 10 nm, and ssDNA at 10 nm, exhibited superior robustness and a higher figure of merit (2287.2 RIU⁻¹), offering consistent performance across a broader dynamic range. These results highlight the versatility of SPR biosensors in adapting to diverse diagnostic needs.

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来源期刊

Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

166

审稿时长

54 days

期刊介绍： Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.