Fluorescence sensing platform based on graphene oxide nucleic acid interaction for detecting mercury ions, bisphenol A, and target DNA

Abstract

A multifunctional fluorescence sensing platform based on graphene oxide (GO) was developed to meet the rapid detection needs of environmental pollutants. Three specific mechanisms were employed to achieve highly sensitive detection. One was to use the T-Hg2+ - T structure to induce the dissociation of double stranded DNA from GO surface, achieving Hg2+ detection with a linear range of 100–1500 nM and a detection limit of 0.110 μ M. The second method was to release the trigger chain through the dissociation of bisphenol A (BPA) adapter complementary chain complex, combined with GO fluorescence quenching characteristics to detect BPA, with a detection limit of 0.5035 μ g/mL. The third method used Exo III enzyme to cleave double stranded DNA for signal amplification, combined with GO differential adsorption detection of nucleic acid fragments to detect target DNA, corresponding to a linear range of 0.01–0.4 nM and a detection limit of 11.2 pM. The spiked recovery rates of the three methods in wastewater/serum samples were 96.82 %–107.49 %, with a relative standard deviation of<3.47 %. The above research method combined nucleic acid recognition characteristics with GO signal regulation function through modular design, providing a new strategy for on-site detection of environmental pollutants.