SPARK - Sensor platform for affinity recognition of paraquat

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X Pub Date : 2025-05-02 DOI:10.1016/j.biosx.2025.100625

Durgasha C. Poudyal , Vikram Narayanan Dhamu , Manish Samson , Sumana Karmakar , Sriram Muthukumar , Shalini Prasad

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引用次数: 0

Abstract

The extensive use of paraquat (PQT) in agriculture has led to considerable environmental pollution and has raised serious health issues. Rapid, sensitive, and on-site detection tools are crucial for monitoring paraquat residue and preventing its toxic effect. This study focused on demonstrating a portable, label-free electrochemical sensor platform (SPARK) for the direct detection of paraquat using edamame soybean as food matrix. The sensor platform was fabricated using the excellent substrate property of a reduced graphene oxide (rGO), which was immobilized with the anti-paraquat antibody (PQT-Ab, specific to recognize the herbicide paraquat) using a linker molecule 1-pyrenebutanoic acid succinimidyl ester (Pyr-BASE). The rate of crosslinker adsorption on rGO was evaluated and demonstrated for the first time using electrochemistry, with the calculated monolayer adsorption of the pyrene-based crosslinker found to be 244 μC/cm². The sensor performance was tested using portable device platform (SPARK) to confirm its feasibility, which showed a limit of detection (LOD) of 0.3 ng/mL (0.3 ppb), dynamic range of PQT from 0.3 to 72.9 ng/mL and an r² value of 0.98. The cross-reactivity study demonstrates high selectivity for the target PQT antigen, in presence of non-specific antigen such as glyphosate and chlorpyrifos. Pearson's correlation of r = 0.99 indicated a strong positive correlation between SPARK platform and the third-party gold standard method. Developed SPARK platform provides sensitive data comparable to traditional analytical methods in a simplified manner, thereby opening the possibility for electrochemical sensor platform to be used as on-site testing devices for various other food matrices.

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用于百草枯亲和力识别的SPARK传感器平台

百草枯（PQT）在农业中的广泛使用导致了相当大的环境污染，并引发了严重的健康问题。快速、灵敏和现场检测工具是监测百草枯残留和预防其毒性作用的关键。本研究主要展示了一种便携式、无标签电化学传感器平台（SPARK），用于以毛豆为食品基质直接检测百草枯。该传感器平台是利用还原氧化石墨烯（rGO）的优良底物特性制成的，该平台与抗百草枯抗体（PQT-Ab，特异性识别除草剂百草枯）固定，并使用连接分子1-芘丁酸琥珀酰基酯（Pyr-BASE）。首次用电化学方法对交联剂在氧化石墨烯上的吸附速率进行了评价和验证，计算得到芘基交联剂的单层吸附量为244 μC/cm2。利用便携式仪器平台（SPARK）对传感器性能进行了测试，结果表明该传感器的检出限（LOD）为0.3 ng/mL (0.3 ppb)， PQT动态范围为0.3 ~ 72.9 ng/mL， r2值为0.98。交叉反应性研究表明，在草甘膦和毒死蜱等非特异性抗原存在的情况下，PQT靶抗原具有高选择性。皮尔逊相关r = 0.99，表明SPARK平台与第三方金标准方法呈正相关。开发的SPARK平台以简化的方式提供了与传统分析方法相当的敏感数据，从而为电化学传感器平台作为各种其他食品基质的现场测试设备打开了可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

166

审稿时长

54 days

期刊介绍： Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.