Biosensors and Bioelectronics: X最新文献

{"title":"Water-soluble organic fluorescence-based probes for biomolecule sensing and labeling","authors":"Aayushi Joshi ,&nbsp;Nandini Mukherjee ,&nbsp;Manoj Pandey","doi":"10.1016/j.biosx.2024.100510","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100510","url":null,"abstract":"<div><p>Fluorescence-based probes have been the key interest of researchers working at the intersection of chemistry and biology. Such probes are crucial for strengthening our understanding about biochemical processes, drug delivery, and fluorescence-guided surgery. A challenge in this regard is optimizing the probe's aqueous solubility while maintaining its lipophilicity to allow cell membrane permeation. This review summarizes the recent progress in water-soluble fluorescence-based probes for different types of biomolecules including carbohydrates, proteins, enzymes, amino acids, neurotransmitters and biologically relevant reactive species. A comprehensive overview of the crucial parameters for such probes' design, potential sensing mechanism for specific analytes, and experimental conditions for sensing has been provided. Incorporation of hydrophilic functional groups, ionic charge(s), absorption-emission characteristics and pH-stability in biological window are pivotal to develop optimized probes with high sensitivity for target biomarkers. We further underline the limitations of the probes that hinder their translation to clinical research and also indicate major research gap in optimizing any single probe for a certain biomarker.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100510"},"PeriodicalIF":10.61,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000748/pdfft?md5=e975c4e3ac51ad99d600a1707114ee43&pid=1-s2.0-S2590137024000748-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141322671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable ion-selective sensors with rapid conditioning and extended stability achieved through modulation of water and ion transport","authors":"Yue Guo,&nbsp;Chaoqi Wang,&nbsp;Ge Han,&nbsp;Hnin Yin Yin Nyein","doi":"10.1016/j.biosx.2024.100509","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100509","url":null,"abstract":"<div><p>Solid-contact (SC) ion-selective electrodes (ISEs) are often employed in wearables for electrolytes detection owing to their simplicity and ease of miniaturization. However, to mitigate their inherently unstable open circuit potential signal, ISEs require long hours of conditioning and frequent calibration prior to and during operation, limiting their practicality in wearable applications. Inspired by strategies to address water crossover and flooding in polyelectrolyte fuel cells, we demonstrated a SCISE with minimal conditioning time and long-term stability by modulating the rate-limiting step between mass transfer of water and hydrated ions and redox kinetics in the conducting polymer (CP). Our strategy comprised a wearable ISE with a superhydrophobic CP, PEDOT:TFPB, which reduced water and ion fluxes within the ISE, resulting in a stable and less-swollen CP and diminished water layer formation while maintaining CP's high capacitance. Our PEDOT:TFPB based ISEs functioned after a short conditioning time of 30 min and exhibited extended stability with a reduced signal deviation of only 0.16 % per hour (0.02 mV h⁻¹) during 48 h of continuous measurement. Through systematic studies, we showed that ISE performance could be further tuned by tailoring the thickness of the ion-selective membrane as well as the hydrophobicity and polymerization charges of the CP. Without the need for recurrent calibration, our ISEs sustain high accuracy and prolonged stability upon integration into a wearable format for on-body perspiration analysis. Our strategy allows wearable ion-selective sensors with minimal maintenance at the user-end for long-term continuous monitoring, unveiling their potential in sports, healthcare, and diagnosis fields.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100509"},"PeriodicalIF":10.61,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000736/pdfft?md5=721972bb729c054e5c8e04be25ebb11e&pid=1-s2.0-S2590137024000736-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141329221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study and characterization of BaFe12O19/PVDF composites as electrode materials for supercapacitors","authors":"Syahrul Humaidi ,&nbsp;Muhammadin Hamid ,&nbsp;Hadi Wijoyo","doi":"10.1016/j.biosx.2024.100507","DOIUrl":"10.1016/j.biosx.2024.100507","url":null,"abstract":"<div><p>Supercapacitors are an interesting energy storage technology to be studied. This research uses mesoporous BaFe₁₂O₁₉ particles and synthesized Polyvinylidene fluoride (PVDF) polymers as materials to obtain high performance supercapacitors. Composites were synthesized by facile one-step method using BaFe₁₂O₁₉ which was prepared through co-precipitation chemical method with a calcination process at 200 °C along with PVDF with variations in sample composition of BaFe₁₂O₁₉, BaFe₁₂O₁₉ 20%, BaFe₁₂O₁₉ 30%, BaFe₁₂O₁₉ 40%, and BaFe₁₂O₁₉ 60%. And finally the fabrication of supercapacitor electrodes is carried out. The result of the synthesized material is distributed grains with the average particle size of each sample ranging from 180 to 185 nm. Then it has the highest peak in crystals with a miller index (114). Furthermore, it has the main functional group, Ba–O with a wave number of 1632 cm⁻¹. Furthermore, the best supercapacitor electrode is BaFe₁₂O₁₉/PVDF 60% which produces an area of 0.51 mVA where the greater the surface area, the higher the capacitance obtained. Then at BaFe₁₂O₁₉/PVDF 60% has the highest power density value at 12.36 Wh/kg and the highest power density value at 299.14 Wh/kg. It is expected that the results obtained can be a reference for further electrode material research.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100507"},"PeriodicalIF":10.61,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000712/pdfft?md5=5aaa737e5018664571ad5c6b1ffd01de&pid=1-s2.0-S2590137024000712-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141415526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vanadium nitride /poly(0-methoxy aniline)- poly(3,4-ethylene dioxythiophene) interpenetrated into nanocomposite for efficient photocatalytic degradation of methylene blue and enhanced electrochemical sensing of mebendazole","authors":"Munusamy Settu ,&nbsp;Govindhan Gnanamoorthy ,&nbsp;Bavani Thirugnanam ,&nbsp;Narayanan Vengidusamy ,&nbsp;Majed A. Alotaibi","doi":"10.1016/j.biosx.2024.100508","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100508","url":null,"abstract":"<div><p>Vanadium nitride-poly (0-methoxy aniline)- poly(3,4-ethylene dioxythiophene) (VN-POMA-PEDOT) hybrid was synthesized via ammonolysis and chemical oxidative polymerization technique using VN-POMA-PEDOT/GCE with electrocatalytic activity has two dimensional VN hierarchical porosity with POMA-PEDOT structure created VN-POMA-PEDOT modified GCE working electrode. Donor-acceptor behavior and double-layer growth enable enhanced electrochemical performance and catalytic activity of mebendazole (MBZ). This work investigated the electrochemical sensing conduct of a VN-POMA-PEDOT hybrid composite towards MBZ. The detection limit (DL) and quantification limit (QL) were determined to be 2.192 × 10⁻⁹ μM μA⁻¹ and 5.245 × 10⁻⁹ M μA⁻¹. Estimation of anti-interference ability, long-term stability, and reproducibility revealed that the prepared VN-POMA-PEDOT electrode is appropriate for the electrochemical sensing finding of MBZ in real analysis, such by way of anti-helminthic drug milk. The VN-POMA-PEDOT achieved 98.9% efficiency in the photocatalytic degradation of methylene blue (MB) within 50 min with degradation rate 8.3 × 10⁻³ min⁻¹. The suppleness of this method was confirmed by the hybrid morphology VN-POMA-PEDOT, which shows an enormously superior and enhanced photocatalytic presentation of MB.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100508"},"PeriodicalIF":10.61,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000724/pdfft?md5=7bd5c24455aaec7844e13a98a85654af&pid=1-s2.0-S2590137024000724-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141322527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aeromonas veronii specific aptamer and peroxidase mimic tyrosine-capped gold NanoZymes enable highly specific sensing of fish pathogenic bacteria","authors":"Dhruba Jyoti Sarkar ,&nbsp;Ayan Biswas ,&nbsp;Shirsak Mondal ,&nbsp;Vijay Kumar Aralappanavar ,&nbsp;Jyotsna Dei ,&nbsp;Swapnil Sinha ,&nbsp;Bijay Kumar Behera ,&nbsp;Ramij Raja ,&nbsp;Soumyadeb Bhattacharyya ,&nbsp;Souvik Pal ,&nbsp;Subhankar Mukherjee ,&nbsp;Vipul Bansal ,&nbsp;Basanta Kumar Das","doi":"10.1016/j.biosx.2024.100505","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100505","url":null,"abstract":"<div><p>Despite major advances in biosensing, quick, dependable, and effective on-site detection of bacterial infections remains a serious issue, owing to a lack of acceptable or appropriate diagnostic platforms. To address this gap, we presented a new colorimetric gold NanoZyme aptasensor for rapid sensing of <em>Aeromonas veronii</em>, an infectious bacterial disease in fish. The <em>A. veronii-specific</em> aptamer (AVS01) was developed through Cell-SELEX. The sensing mechanism involves inhibition of AuNPs induced peroxidase-mimic catalytic activity through surface adsorption by AVS01 which in the presence of the <em>A. veronii</em> desorb from the AuNPs allowing recovery of the catalytic activity leading to colorimetric response, whereas the sensor is insesnsitive to other nontarget bacterial cells. This method is very specific and sensitive, allowing for the quick and visible sensing of <em>A. veronii</em> with a detection limit of 1281 CFU mL⁻¹ within 15 min. The method has great potential for rapid diagnosis of bacterial infection in fish caused by <em>A. veronii</em>.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100505"},"PeriodicalIF":10.61,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000694/pdfft?md5=4eae64d853db566dc9cedf174ca0c16d&pid=1-s2.0-S2590137024000694-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141322672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensor with combined plasmonic and magnetic activities","authors":"Raju Sapkota ,&nbsp;Sadna Isik ,&nbsp;Hery Suyanto ,&nbsp;Ni Nyoman Rupiasih ,&nbsp;Nahara Ingles ,&nbsp;Conrad Rizal","doi":"10.1016/j.biosx.2024.100506","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100506","url":null,"abstract":"<div><p>Sensors utilizing magneto-optical surface plasmon resonance are gaining increasing scientific and practical attention to detect atmospheric gases and humidity. The magneto-optic surface plasmon resonance wavelength is defined by the plasmonic structure's geometry and structure, making it immune to electromagnetic interference outside its resonance frequency range. The present study investigates their application for the detection of atmospheric gases including humidity. In contrast to conventional sensors, magneto-optic sensors exhibited excellent performance in terms of sensitivity (10 times greater), higher quality factor (up to 76 times higher) and design simplicity in terms of layer thickness optimization, integration, and robustness. These results suggest significant potential for utilization of magneto-optic sensors across multiple industries.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100506"},"PeriodicalIF":10.61,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000700/pdfft?md5=1b0217d1ce0a87eace49c2d3adee1cec&pid=1-s2.0-S2590137024000700-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141297978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-based digital point-of-care panel assay with enzymatic catalytic reaction","authors":"Kiran N. Roest ,&nbsp;Matthew Lee ,&nbsp;Jon Rees ,&nbsp;Vladimir Gubala","doi":"10.1016/j.biosx.2024.100504","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100504","url":null,"abstract":"<div><p>The recent pandemic improved awareness amongst the public of the need for rapid blood tests for community and home settings. In this work, we evaluated the performance of a digital, lipid panel test in microfluidic assay format which can be read using a smartphone camera. The PocDoc Lipid test is embedded within a cardiovascular screening application that utilizes the QRISK3 risk prediction algorithm to determine an individual's risk of having a cardiovascular event in the next 10 years and their healthy heart age. The test can be used to screen for individuals at risk of hyperlipidemia (e.g. high total cholesterol or triglycerides) and for individuals at high risk of cardiovascular disease at home or in community or surgery settings. The device was evaluated in a performance evaluation study, using 125 whole blood samples, following CLSI guidelines. Performance evaluation of the PocDoc device demonstrated accuracy that meets international NCEP guidelines and that is on par with other point-of-care tests. Sensitivity and specificity analysis supports the use of PocDoc to identify patients with hyperlipidemia or at high risk of cardiovascular disease. Bland-Altman analysis suggests that this point-of-care device can be used as an alternative to venous blood collection. This single-step model for cardiovascular disease risk measurement which can be done at home or in community settings may improve cardiovascular disease prevention.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100504"},"PeriodicalIF":10.61,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000682/pdfft?md5=f33458f1e87d0e8032e4bf7bd4fcb148&pid=1-s2.0-S2590137024000682-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141289319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in wearable electrochemical biosensors towards technological and material aspects","authors":"Mahan Hosseinzadeh Fakhr ,&nbsp;Ivan Lopez Carrasco ,&nbsp;Dmitry Belyaev ,&nbsp;Jihun Kang ,&nbsp;YeHyeon Shin ,&nbsp;Jong-Souk Yeo ,&nbsp;Won-Gun Koh ,&nbsp;Jeongwoo Ham ,&nbsp;Alexander Michaelis ,&nbsp;Joerg Opitz ,&nbsp;Natalia Beshchasna","doi":"10.1016/j.biosx.2024.100503","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100503","url":null,"abstract":"<div><p>The next generation of wearable biosensors comes with the latest advancements in biosensor technology. Soft and stretchable electrode materials like hydrogels with the similar functionalities of human tissue including stretchability, self-healability, and responsiveness to different stimuli have emerged as the most versatile materials in wearable electronics. The incorporation of conductive nanofillers is found to enhance the sensitivity of the electrochemical biosensors significantly. Microfluidic technology has reduced the volume of samples and reagents required for the analysis, allowing continuous biomedical monitoring from a drop of biofluid. In this paper, the most advanced progress in electrochemical wearable platforms that can noninvasively and continuously monitor the biochemical markers in body fluids for the diagnosis and health management is reviewed. Innovation in microelectronics, modification, fabrication technologies, and detection methods are the main focus of the discussion. In particular, hydrogel-based sensors and microfluidic systems as the latest technology trends in wearable detection are discussed in detail. Integration of miniaturized electrochemical wearable biosensors with wireless technology as a great promise for real-time healthcare monitoring and point-of-care (POC) diagnostics is also summarized. Finally, we outline the most advanced wearable biosensors with optimized material and design as well as key challenges that need to be addressed to improve sensing performance (accuracy, sensitivity, selectivity, stability), portability (miniaturized size and light weight), and flexibility of the wearable biosensors.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100503"},"PeriodicalIF":10.61,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000670/pdfft?md5=884e47b94f329229f5631b2585fe9d41&pid=1-s2.0-S2590137024000670-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141249515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of microchip electrophoresis pulsed amperometric detector-based soil pesticide analyser","authors":"Rishi Raj ,&nbsp;Suddhasatwa Basu ,&nbsp;Sandeep Kumar Jha","doi":"10.1016/j.biosx.2024.100502","DOIUrl":"10.1016/j.biosx.2024.100502","url":null,"abstract":"<div><p>The present study involved the fabrication and testing of a Microchip electrophoresis (MCE) device for pulse amperometry based detection of pesticides from their mixture. We were able to separate and then quantify three distinct types of insecticides, namely Chlorpyrifos, Imidacloprid, and Fipronil using on chip MCE followed by pulsed amperometric detection. All these results were obtained with an inhouse developed potentiostat cum controller unit with a detection time of only 15 min, employing a minimal sample size of 2 μL without any preconcentration or extraction procedure. The limit of detection (LOD) was calculated as 42.69 μM, 62.61 μM, and 71.14 μM or 14.96, 16.0 and 31.09 ppm, respectively for Chlorpyrifos, Imidacloprid, and Fipronil and their respective migration times as 536 ± 6.3 s, 484 ± 1.7 s, and 604 ± 3.5 s (n = 14). The sensitivity of detection was determined as 0.03 nA/μM for Chlorpyrifos, 0.0265 nA/μM for Imidacloprid, and 0.035 nA/μM for Fipronil. In addition, the efficacy of the produced microchip was confirmed by analysing soil extract spiked with known pesticides concentrations while the recovery percentage, representing a ratio of calculated concentration to spiked concentration multiplied by hundred was found as 84.3% (±9.4%) (n = 9). Thus, integrating microchip technology with the developed analytical instruments presents significant promise for practical field applications and the analysis of diverse analytes by way of creating a library where the migration coefficient and peak detection current are needed for any analyte which can be made cationic or anionic using a suitable buffer.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100502"},"PeriodicalIF":10.61,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000669/pdfft?md5=c3b3cbcebf197ef8233415b537d5141d&pid=1-s2.0-S2590137024000669-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141194588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable technology for one health: Charting the course of dermal biosensing","authors":"Georgeta Vulpe ,&nbsp;Guoyi Liu ,&nbsp;Sam Oakley ,&nbsp;Dimitrios Pletsas ,&nbsp;Guanghao Yang ,&nbsp;Rosa Dutra ,&nbsp;Owen Guy ,&nbsp;Yufei Liu ,&nbsp;Mark Waldron ,&nbsp;Joe Neary ,&nbsp;Arjun Ajith Mohan ,&nbsp;Sanjiv Sharma","doi":"10.1016/j.biosx.2024.100500","DOIUrl":"10.1016/j.biosx.2024.100500","url":null,"abstract":"<div><p>Over the last decade, a significant paradigm shift has been observed towards leveraging less invasive biological fluids—such as skin interstitial fluid (ISF), sweat, tears, and saliva—for health monitoring. This evolution seeks to transcend traditional, invasive blood-based methods, offering a more accessible approach to health monitoring for non-specialized personnel. Skin ISF, with its profound resemblance to blood, emerges as a pivotal medium for the real-time, minimally invasive tracking of a broad spectrum of biomarkers, thus becoming an invaluable asset for correlating with blood-based data. Our exploration delves deeply into the development of wearable molecular biosensors, spotlighting dermal sensors for their pivotal roles across both clinical and everyday health monitoring scenarios and underscoring their contributions to the holistic One Health initiative. In bringing forward the myriad challenges that permeate this field, we also project future directions, notably the potential of skin ISF as a promising candidate for continuous health tracking.</p><p>Moreover, this paper aims to catalyse further exploration and innovation by presenting a curated selection of seminal technological advancements. Amidst the saturated landscape of analytical literature on translational challenges, our approach distinctly seeks to highlight recent developments. In attracting a wider spectrum of research groups to this versatile domain, we endeavour to broaden the collective understanding of its trajectory and potential, mapping the evolution of wearable biosensor technology. This strategy not only illuminates the transformative impact of wearable biosensors in reshaping health diagnostics and personalized medicine but also fosters increased participation and progress within the field. Distinct from recent manuscripts in this domain, our review serves as a distillation of key concepts, elucidating pivotal papers that mark the latest advancements in wearable sensors. Through presenting a curated collection of landmark studies and offering our perspectives on the challenges and forward paths, this paper seeks to guide new entrants in the area. We delineate a division between wearable epidermal and subdermal sensors—focusing on the latter as the future frontier—thereby establishing a unique discourse within the ongoing narrative on wearable sensing technologies.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"19 ","pages":"Article 100500"},"PeriodicalIF":10.61,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000645/pdfft?md5=e560ae9f111b111632d58b48d0376254&pid=1-s2.0-S2590137024000645-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141130332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}