Biosensors and Bioelectronics: X最新文献_第8页

Modeling of a BK7 prism/Ag/Ni/ZnSe/SM-based sensor for maintaining optimum performance in glucose level detection BK7棱镜/Ag/Ni/ZnSe/ sm传感器在葡萄糖水平检测中的最佳性能建模

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-03-11 DOI: 10.1016/j.biosx.2025.100604

Kazi Mustafizur Rahman

{"title":"Modeling of a BK7 prism/Ag/Ni/ZnSe/SM-based sensor for maintaining optimum performance in glucose level detection","authors":"Kazi Mustafizur Rahman","doi":"10.1016/j.biosx.2025.100604","DOIUrl":"10.1016/j.biosx.2025.100604","url":null,"abstract":"<div><div>Patients suffering from diabetes, or having problems regarding up-downs in glucose levels may need to prick human skin to draw blood samples for checkups. Due to the enhancement of photonics, surface plasmon resonance (SPR) is a relief to them. As of now, glucose levels can be detected from urine instead of blood. This research delves into the characterization of an SPR sensor combining a prism-coupled Silver (Ag), Nickel (Ni), and Zinc Selenide (ZnSe) hybrid modeled structure to maintain overall enhanced performance for the detection of glucose concentrations of the patients in urine samples. The motivation is to develop an optimum resulting surface plasmon resonance sensor for this issue based on the Kretschmann configuration. In the present work, the standard transfer matrix method and the Fresnel formulations were utilized to investigate this study. The proposed biosensor comprises nickel (Ni) and zinc selenide (ZnSe), which improves the sensitivity of the surface plasmon resonance-based sensor and protects Ag from being oxidized. The findings reveal high-overall performance parameters are a sensitivity of 329.21°/RIU, full width at half maximum of 3.89 (degrees), a figure of merit of 84.489398, a quality factor of 84.575835 (1/RIU), and a detection accuracy of 0.2570694 (1/degree) for the BK7 prism/Ag/Ni/ZnSe/sensing medium configuration at a wavelength of 633 nm. The exalted performance of this biosensor is efficient in detecting the concentration level of different liquid analytes in biosensing platforms, offering significant promise for the analysis of glucose concentration.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100604"},"PeriodicalIF":10.61,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cereals as sources of lysine in the reformulation of meat products. Evaluation using a biosensor 谷物作为赖氨酸的来源在肉类产品的重新配方。使用生物传感器进行评估

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-03-03 DOI: 10.1016/j.biosx.2025.100592

Erika Alvarez Cañarte , Guilber Vergara Velez , Frank Guillermo Intriago Flor , Efrain Pérez Vega , Miguel Andrès Falconi Vèlez , Delia Noriega Verdugo , Génesis Pamela García García , Livis Sharith Díaz Alarcón , Andrés Miguel Anchundia Loor , Carlos Jadán-Piedra , Felipe Jadán Piedra

{"title":"Cereals as sources of lysine in the reformulation of meat products. Evaluation using a biosensor","authors":"Erika Alvarez Cañarte , Guilber Vergara Velez , Frank Guillermo Intriago Flor , Efrain Pérez Vega , Miguel Andrès Falconi Vèlez , Delia Noriega Verdugo , Génesis Pamela García García , Livis Sharith Díaz Alarcón , Andrés Miguel Anchundia Loor , Carlos Jadán-Piedra , Felipe Jadán Piedra","doi":"10.1016/j.biosx.2025.100592","DOIUrl":"10.1016/j.biosx.2025.100592","url":null,"abstract":"<div><div>The energy and protein requirements of the population must be met, and the use of new analytical methods for rapid, low-cost detection of essential elements like lysine in reformulated foods is crucial. In this context, conditions were evaluated to develop a biosensor with lysine alpha oxidase (LOx), which showed high affinity for lysine with a K<sub>M</sub> of 0.32 mM. Different concentrations of cereals and legumes (70-30; 55-45; 85-15; quinoa-Lablab Purpureus; pole beans-Lablab Purpureus; and rye-Lablab Purpureus) were incorporated into meat sausages to enrich lysine, achieving a significant increase in lysine concentration (up to 75%) when 15% quinoa was substituted. The potentiometric signal, related to oxygen consumption during lysine oxidation, was detected at 15 s using a voltage of −600 mV. The biosensor, coupled with the immobilized enzyme, allowed the use of low volumes. A positive relationship was found between oxygen consumption (mg O<sub>2</sub>/L∗s-1) and lysine concentration in the range of 0.01–0.2 mM, with an R<sup>2</sup> of 0.9964. The immobilized enzyme-based sensor demonstrated good sensitivity (0.01 mM) and the membrane could be reused up to 18 times, maintaining 92% of its initial activity after 70 days. The biosensor method showed minimal residue formation and had a strong correlation with high-performance liquid chromatography (HPLC) results, validating its accuracy.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100592"},"PeriodicalIF":10.61,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction notice to “Multiple and simultaneous detection for cytokines based on the nanohole array by electrochemical sandwich immunoassay” [Biosens Bioelectron.: X 14 (2023) 100387] “基于纳米孔阵列的电化学三明治免疫分析法对细胞因子的多重同时检测”撤回通知[Biosens Bioelectron]。[x 14 (2023) 100387]

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-27 DOI: 10.1016/j.biosx.2025.100598

Han Na Suh , Sung-Hoon Yoon , Yoo Min Park , HyungJin Kim , SangHee Kim , JuKyung Lee

引用次数: 0

Conductive polymer composites in soft robotics, flexible sensors and energy storage: Fabrication, applications and challenges 软机器人、柔性传感器和储能中的导电聚合物复合材料：制造、应用和挑战

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-25 DOI: 10.1016/j.biosx.2025.100597

Hassan Tawsif Tazwar , Maisha Farzana Antora , Itmam Nowroj , Adib Bin Rashid

{"title":"Conductive polymer composites in soft robotics, flexible sensors and energy storage: Fabrication, applications and challenges","authors":"Hassan Tawsif Tazwar , Maisha Farzana Antora , Itmam Nowroj , Adib Bin Rashid","doi":"10.1016/j.biosx.2025.100597","DOIUrl":"10.1016/j.biosx.2025.100597","url":null,"abstract":"<div><div>Conductive Polymer Composites (CPCs) have emerged as promising materials with applications in soft robotics, flexible sensors, and energy storage. This review paper begins with a brief introduction to the available polymers and additives in context of CPCs, followed by a classification of the resulting composites, setting the stage for further discussions. Then we sequentially analyze the fabrication techniques and practical applications of CPC in the aforementioned sectors highlighting their performance. Furthermore, the review delves into critical aspects such as failure modes, including interfacial debonding and fatigue, the environmental impact and sustainability of CPCs, considering the source and biodegradability of polymers and the environmental implications of additives. Limitations such as particle leakage in metal-based flexible devices, nanoparticle agglomeration and high cost of modern fabrication techniques have been discussed. Future research directions, including development of cost-effective manufacturing methods and proper encapsulation techniques for flexible devices have been highlighted.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100597"},"PeriodicalIF":10.61,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of plant pathogenic bacteria at subspecies level using a dielectrophoresis device combined with Raman spectroscopy 利用介电联用拉曼光谱技术在亚种水平上鉴定植物致病菌

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-20 DOI: 10.1016/j.biosx.2025.100595

Alessio Sacco , Camilla Sacco Botto , Chiara D'Errico , Marina Ciuffo , Slavica Matić , Giulia Molinatto , Andrea M. Giovannozzi , Andrea M. Rossi , Emanuela Noris

{"title":"Characterization of plant pathogenic bacteria at subspecies level using a dielectrophoresis device combined with Raman spectroscopy","authors":"Alessio Sacco , Camilla Sacco Botto , Chiara D'Errico , Marina Ciuffo , Slavica Matić , Giulia Molinatto , Andrea M. Giovannozzi , Andrea M. Rossi , Emanuela Noris","doi":"10.1016/j.biosx.2025.100595","DOIUrl":"10.1016/j.biosx.2025.100595","url":null,"abstract":"<div><div>Timely diagnosis of plant diseases and correct identification of etiological agents are fundamental to guarantee quality and quantity of agricultural products and food. Phytopathogenic bacteria induce devastating effects on crops. Their diagnosis and identification, mainly based on serological and molecular tools, are time consuming and expensive processes and require trained personnel. Among the innovative methods providing rapid, accurate, and reliable diagnosis at reduced costs, Raman spectroscopy (RS) is gathering considerable attention. RS provides a direct and non-destructive platform to gather information on the chemical and biochemical components of a sample, such as microorganism cultures, revealing their biological role. Due to the weak signals of bacterial cells in RS, a dielectrophoresis (DEP) approach was adopted to amplify the bacterial signals. Using Raman-DEP analysis, a dataset of spectra from different harmful phytopathogenic bacteria belonging to the genera <em>Pseudomonas</em> spp., <em>Xanthomonas</em> spp., and <em>Erwinia</em> spp. was obtained. Machine learning approaches were employed to discriminate isolates at the genus, species, and unprecedentedly at the pathovar level, reaching accuracies, precisions, recalls, and F1 scores of 94–100%. This approach offers important advancements in the non-destructive and rapid classification of microorganisms and is suitable to be readily extended to environmental and food diagnostics.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100595"},"PeriodicalIF":10.61,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and application of enzyme-based hydrogels 酶基水凝胶的制备与应用

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-20 DOI: 10.1016/j.biosx.2025.100594

Yahui Wen , Xinghai Wang , Jinxue Zhao , Xuejing Zhai , Wei Xia , Peiyi Li , Keqiang Lai , Lidong Wu

引用次数: 0

Nanostructured immunosensing system for label-free impedimetric detection of multiple breast cancer biomarkers (CEA and HER2) using CoMoO4@PANI-PPy Nanocomposite 纳米结构免疫传感系统用于无标记检测多种乳腺癌生物标志物（CEA和HER2），使用CoMoO4@PANI-PPy纳米复合材料

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-20 DOI: 10.1016/j.biosx.2025.100596

Omar Ramadan, Rabeay Y.A. Hassan

{"title":"Nanostructured immunosensing system for label-free impedimetric detection of multiple breast cancer biomarkers (CEA and HER2) using CoMoO4@PANI-PPy Nanocomposite","authors":"Omar Ramadan, Rabeay Y.A. Hassan","doi":"10.1016/j.biosx.2025.100596","DOIUrl":"10.1016/j.biosx.2025.100596","url":null,"abstract":"<div><div>Breast cancer patients often exhibit positive expression of selective tumor biomarkers including the carcinoembryonic antigen (CEA), and human epidermal growth factor receptor 2 (HER2). These potential protein biomarkers are highly crucial for the selective and early tumor diagnosis. In this study, disposable dual impedimetric immunosensing systems were constructed using the cobalt molybdate (CoMoO<sub>4</sub>) and polyaniline/polypyrrole (PANI-PPy) nanocomposite, for the rapid quantification of CEA and HER2 concentration levels. The designed sensing probes were fabricated by chemically conjugating two-targeting antibodies (anti-CEA and anti-HER2) on the nanostructured electrode surfaces modified with (AuNPs/CoMoO<sub>4</sub>@PANI-PPy). These successful chemical antibody immobilizations were enabled by the use of 4-aminothiophenol (4-ATP) as a cross-linking agent. The impedimetric immunosensing assay was fully optimized by screening of nanomaterials, determining the optimal ratio of the nanocomposite, adjusting the time of self-assembled monolayer (SAM) formation, and identifying the degree of cross-reactivity with potential non-targeting molecules. Accordingly, the biosensors demonstrated distinguished linear dynamic ranges of 1.0 fg/mL to 100 ng/mL for the CEA and 25 fg/mL to 100 ng/mL for the HER2, with detection limits of 8.2 fg/mL and 4.9 fg/mL for CEA and HER2, respectively. The immunesensor's reproducibility and stability were studied, showing minimal variation in charge transfer resistance (RSD = 1.33%, n = 5) and maintaining 82.5% functionality after four weeks of storage at 4 °C. These findings highlighted the potential use of these biosensor chips for clinical applications in early breast cancer detection and rapid diagnosis.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100596"},"PeriodicalIF":10.61,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A deeper evaluation of cytokeratin fragment 21-1 as a lung cancer tumor marker and comparison of different assays 细胞角蛋白片段21-1作为肺癌肿瘤标志物的深入评价及不同检测方法的比较

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-17 DOI: 10.1016/j.biosx.2025.100593

Dianna J. Rowe , Timothy A. Khalil , Michael N. Kammer , Caroline M. Godfrey , Yong Zou , Cindy L. Vnencak-Jones , David Xiao , Stephen Deppen , Eric L. Grogan

{"title":"A deeper evaluation of cytokeratin fragment 21-1 as a lung cancer tumor marker and comparison of different assays","authors":"Dianna J. Rowe , Timothy A. Khalil , Michael N. Kammer , Caroline M. Godfrey , Yong Zou , Cindy L. Vnencak-Jones , David Xiao , Stephen Deppen , Eric L. Grogan","doi":"10.1016/j.biosx.2025.100593","DOIUrl":"10.1016/j.biosx.2025.100593","url":null,"abstract":"<div><div>Studies show CYFRA 21-1 fragments of cytokeratin 19 (CK19) to be promising biomarkers for non-small cell lung cancer (NSCLC). Although previous literature identifies specific CYFRA 21-1 antibody binding epitopes, the exact molecular weight of the CK19 fragment being detected by current assays is not well-documented. Serum samples from 58 patients (lung cancer (N = 36), control (N = 22)) were used to measure CYFRA 21-1 across four different quantification assays: enzyme-linked immunosorbent assay (ELISA), chemiluminescent assay (ChLIA), electrochemiluminescence immunoassay (ECLIA), and compensated interferometric reader (CIR). In the cancer group, correlation between ECLIA and ELISA was high (R<em>(Pearson)</em> = 0.948, r<em>(Spearman)</em> = 0.868) while correlation between ECLIA vs ChLIA and ECLIA vs CIR was low (R<em>=</em> 0.005, r = −0.0593), (R = 0.0275, r = 0.167), respectively. In the control group, correlation between ECLIA and ELISA was high (R = 0.948, r = 0.868) while correlation between ECLIA vs ChLIA and ECLIA vs CIR was low (R = 0.005, r = −0.0593), (R = 0.0275, r = 0.167), respectively. Compared to ECLIA, concordance coefficients <strong>(</strong><em>p</em><sub><em>c</em></sub>) were poor (<em>p</em><sub><em>c</em></sub> < 0.90) across all assays except for cancers group in ELISA (<em>p</em><sub><em>c</em></sub> = 0.913). ECLIA was the only assay to report control ranges above 1 ng/mL CYFRA 21-1 (ECLIA, 1.14–21.59 ng/mL; ELISA, 0.79–24.26 ng/mL; ChLIA, 0.062–0.691 ng/mL; 0.08–7.68 ng/mL). Differing sizes of the protein being measured by each assay may have a role in the discrepancies observed. Given the different CYFRA 21-1 concentration estimates among assays, further characterization of the fragment and its release during epithelial malignancies, such as NSCLC, is imperative to developing effective biomarker assays.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100593"},"PeriodicalIF":10.61,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A liquid biopsy algorithm exploiting local exosome heterogeneity 利用局部外泌体异质性的液体活检算法

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-16 DOI: 10.1016/j.biosx.2025.100585

Taekmin Kim, Dayeon Choi, Soonjoung Kwon, Se-Eun Park, Jongki Kim, Yoon-Ho Lee, Seung-Cheol Choi, Se-Hwan Paek

{"title":"A liquid biopsy algorithm exploiting local exosome heterogeneity","authors":"Taekmin Kim, Dayeon Choi, Soonjoung Kwon, Se-Eun Park, Jongki Kim, Yoon-Ho Lee, Seung-Cheol Choi, Se-Hwan Paek","doi":"10.1016/j.biosx.2025.100585","DOIUrl":"10.1016/j.biosx.2025.100585","url":null,"abstract":"<div><div>We developed a novel cancer detection method that leverages changes in local exosome heterogeneity to regulate bulk-scale heterogeneity in body fluids. Using an automated, reversible isolation system and downstream immunoassays, we investigated exosome subclasses exhibiting marker composition changes associated with cancer. We introduced an index, R, to measure local heterogeneity changes, quantifying the proportion of a third marker within double-marker-positive subclasses. This method, which employs pan-exosome tetraspanins CD9, CD63, and CD81, revealed distinct heterogeneity differences between normal and cancer samples. These differences were consistently demonstrated in exosome samples derived from various cell lines and human sera. Notably, in clinical samples, R values distributed samples from healthy donors and cancer patients into unique patterns based on the third marker within specific subclasses. However, across subclasses, R value changes were significantly smaller in healthy samples than in cancer samples. Sorting patterns were simulated using exosomes derived from immune cells, indicating an immune response contribution to the observed signals. These findings highlight the method's potential for identifying biomarkers for specific cancer diagnoses and multi-cancer screening.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100585"},"PeriodicalIF":10.61,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic impedance sensing distinguishes cancer cell states: A step towards point-of-care diagnostics 微流控阻抗感应区分癌细胞状态：迈向即时诊断的一步

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-02-10 DOI: 10.1016/j.biosx.2025.100589

Mahtab Kokabi , Gulam M. Rather , Mehdi Javanmard

{"title":"Microfluidic impedance sensing distinguishes cancer cell states: A step towards point-of-care diagnostics","authors":"Mahtab Kokabi , Gulam M. Rather , Mehdi Javanmard","doi":"10.1016/j.biosx.2025.100589","DOIUrl":"10.1016/j.biosx.2025.100589","url":null,"abstract":"<div><div>In this study, we employed a custom microfluidic sensor to measure the electrical impedance of cancer cells, aiming to differentiate between suspension and adherent phenotypes. We investigated three suspension cell lines—JeKo-1, MM-1R, and Maver-1—representing hematological malignancies, and two adherent breast cancer cell lines—MDA-MB-231 and MDA- MB-468. The impedance measurements revealed significant differences corresponding to the cells’ growth patterns. Suspension cells exhibited lower median impedance values compared to adherent cells, likely due to variations in cell size, complexity, and membrane properties. Suspension cells, being smaller and less structurally complex, demonstrated reduced impedance, whereas adherent cells, which are larger and form stronger surface attachments, displayed higher impedance values. These findings highlight the potential of electrical impedance as a tool for distinguishing cancer cell types. To further validate these results, we propose focusing on the transition of a single cancer cell type between adherent and suspension states, simulating in vitro cancer proliferation conditions. This approach will provide a deeper understanding of how electrical properties evolve during this transition and may offer insights into distinguishing between benign and metastatic cancers or assessing metastatic stages. The observed reduction in impedance during the adherent-to-suspension transition supports the potential diagnostic utility of this method. Overall, this study demonstrates that electrical impedance provides a rapid, label-free approach for enhancing cancer cell diagnostics based on distinct electrical characteristics.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"23 ","pages":"Article 100589"},"PeriodicalIF":10.61,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0