Aquatic Toxicology最新文献

{"title":"Exploring the combined toxicity of boron nitride nanosheets, cadmium and natural organic matter on Daphnia magna","authors":"Gabriela Helena da Silva ,&nbsp;Jing Ji ,&nbsp;Marcella Torres Maia ,&nbsp;Davide Mattia ,&nbsp;Diego Stéfani Teodoro Martinez","doi":"10.1016/j.aquatox.2024.107198","DOIUrl":"10.1016/j.aquatox.2024.107198","url":null,"abstract":"<div><div>The increase in anthropogenic activities has led to the release of numerous chemicals and pollutants into aquatic ecosystems, raising significant concerns for water quality and health. Among the emerging issues is the interaction between pollutants and nanomaterials (mixture effects). In this work, it was studied the combined toxicity of boron nitride nanosheets (BNNS) and cadmium (Cd²⁺) incorporating the influence of natural organic matter (NOM) to enhance ecological relevance for the first time. Colloidal stability studies showed that BNNS is highly unstable, aggregating and precipitating over time in mineral reconstituted water. However, the addition of natural organic matter stabilizes BNNS. Acute toxicity results showed that this material has a good biocompatibility with <em>D. magna</em>, not causing acute toxic effect (immobility) even at high concentration (100 mg <em>L</em>⁻¹). Moreover, when combined with cadmium, BNNS exhibited a \"Trojan horse\" effect, enhancing Cd²⁺ toxicity by facilitating its uptake at 1 mg <em>L</em>⁻¹. 48h-EC₅₀ values of Cd²⁺ and BNNS+Cd²⁺ were 0.21 and 0.14 mg <em>L</em>⁻¹, respectively. Nevertheless, NOM (10 mg <em>L</em>⁻¹) mitigated this combined toxicity effect after 48 h of exposure. These findings provide novel insights into nanomaterial-pollutant interactions linked to toxicological effects in aquatic environments, contributing to the risk assessment for the safe and sustainable development of the emerging boron nitride nanomaterials and novel products.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107198"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the spatiotemporal distribution, bioaccumulation, and ecological risk assessment of microplastics in aquatic ecosystems: A review","authors":"Tianyu Zhuo ,&nbsp;Beibei Chai ,&nbsp;Xue-yi You","doi":"10.1016/j.aquatox.2024.107210","DOIUrl":"10.1016/j.aquatox.2024.107210","url":null,"abstract":"<div><div>Microplastic (MP) pollution poses a significant threat to aquatic ecosystems. Numerical modeling has emerged as an effective tool for predicting the distribution, accumulation, and risk assessment of MPs in aquatic ecosystems. However, published work has not systematically assessed the strengths and weaknesses of various modeling approaches. Therefore, we conducted a thorough review of the main modeling approaches for MPs over the past six years. We classified the approaches into three categories as: spatial and temporal distribution, bioaccumulation, and systematic ecological risk assessment. The review analyzed application scenarios, modeling methods, and the advantages and disadvantages of models. The results indicate that the accurate simulation of MPs spatial and temporal distribution requires reasonable parameterization and comprehensive transport considerations. Meanwhile, it is important to focus on coupling process models with other types of models. To enhance risk assessment models, expanding the relevant evaluation indicators is essential.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107210"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatotoxic effects of environmentally relevant concentrations of polystyrene microplastics on senescent Zebrafish (Danio rerio): Patterns of stress response and metabolomic alterations","authors":"Pengfei Xie ,&nbsp;Mohammad Mehdi Ommati ,&nbsp;Deshan Chen ,&nbsp;Weijun Chen ,&nbsp;Lei Han ,&nbsp;Xinquan Zhao ,&nbsp;Hongwei Wang ,&nbsp;Shixiao Xu ,&nbsp;Ping Sun","doi":"10.1016/j.aquatox.2025.107252","DOIUrl":"10.1016/j.aquatox.2025.107252","url":null,"abstract":"<div><div>The hepatotoxicity of microplastics (MPs) has garnered increasing attention, but their effects on elderly organisms remain inadequately characterized, particularly concerning hepatic stress response patterns in environmental conditions. In this study, a 10-day exposure period of elderly zebrafish to polystyrene microplastics (PS-MPs, 1 µm) was conducted, with exposure concentrations set at 5.6 × 10^–7 µg/L, 5.6 × 10^–4 µg/L, and 5.6 × 10^–1 µg/L. PS-MPs-induced toxicity varied with concentration: superoxide dismutase (SOD), complement 3 (C3), and complement 4 (C4) initially decreased before rising; 8‑hydroxy-2-deoxyguanosine (8-OhdG), interleukin-6 (IL-6), and interleukin-8 (IL-8) increased at high concentrations. Additionally, catalase (CAT) activity and thiobarbituric acid reactive substances (TBARS) contents rose with concentration. The aged zebrafish liver exhibited differentiation driven by responsiveness; low levels cause homeostatic disruption, and high levels induce genotoxicity and immune activation. LC-MS identified twelve crucial metabolites involved in 18 metabolic pathways, including amino acids (L-tyrosine, l-arginine), lipids (phospholipids, 12(<em>S</em>)-leukotriene B4 and triglycerides), and N-acetylneuraminic acid, related to energy, immunity, and neurological health. Overall, elderly zebrafish exhibited clear dose-dependent thresholds and distinct physiological stress responses under varying concentrations of PS-MPs. These findings reveal how PS-MP exposure can affect physiological health and metabolism, offering critical insights into the ecological risks faced by aging organisms.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107252"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of Linear Alkylbenzene (LABs) on ecosystems: Detection, fate and remediation","authors":"Sadeq Abdullah Abdo Alkhadher ,&nbsp;Lariyah Mohd Sidek ,&nbsp;Mohamad Pauzi Zakaria ,&nbsp;Maged Al-gradi ,&nbsp;Suhaimi Suratman ,&nbsp;Mohammad Sherjeel Javed Khan ,&nbsp;Hidayah Basri ,&nbsp;Mohd Hafiz Zawawi ,&nbsp;Najat Masood ,&nbsp;Tonni Agustiono Kurniawan ,&nbsp;Sami Magam","doi":"10.1016/j.aquatox.2025.107254","DOIUrl":"10.1016/j.aquatox.2025.107254","url":null,"abstract":"<div><div>This review article provides a thorough examination of an interaction between linear alkylbenzenes (LABs) and ecosystems. The review covers various aspects of LABs' impact on ecosystems, focusing on detection and treatment strategies to mitigate ecological consequences. It delves into LABs' role as molecular markers for sewage pollution, their physicochemical properties contributing to persistence, and their effects on aquatic and terrestrial organisms, including disruptions to endocrine systems. The diverse sources of LABs, including domestic wastewater and industrial effluents, are explored, along with their ratios in different matrices for assessing contamination origins. Biodegradation pathways of LABs, both aerobic and anaerobic, are scrutinized, considering their interaction with microbes. Distribution patterns in aquatic environments are discussed, encompassing sediment, water, sewage, and soils. An investigation is conducted on the relationship between LABs and total organic carbon (TOC) as a means of evaluating sewage pollution. It is assessed how sewage treatment facilities (STPs) contribute to biodegradation.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107254"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lithium enhanced plasmid-mediated conjugative transfer of antimicrobial resistance genes in Escherichia coli: Different concentrations and mechanisms","authors":"Jiaxing Li ,&nbsp;Dongzhe Sun ,&nbsp;Jiayi Wu ,&nbsp;Fen Liu ,&nbsp;Yaqi Xu ,&nbsp;Yuanhao Wang ,&nbsp;Xiaoxi Shui ,&nbsp;Qingyang Li ,&nbsp;Baohua Zhao","doi":"10.1016/j.aquatox.2025.107263","DOIUrl":"10.1016/j.aquatox.2025.107263","url":null,"abstract":"<div><div>Conjugative transfer, a pivotal mechanism in the transmission of antimicrobial resistance genes, is susceptible to various environmental pollutants. As an emerging contaminant, lithium (Li) has garnered much attention due to its extensive applications. This research investigated the effects of Li on conjugative transfer process, examining biochemical and omics perspectives. Results revealed that Li could increase the conjugative transfer frequency of both donor and recipient via different mechanisms at varying concentrations. At 0.1 mg/L LiCl, a notable increase in conjugative transfer frequency occurred without ROS elevation. However, the surge of ROS was identified as a crucial regulator at 100 mg/L LiCl, as eliminating ROS would significantly decrease the conjugative transfer frequency. Besides, comparative transcriptome analysis revealed consistent variations in “SOS response”, “quorum sensing” and “oxidative phosphorylation” pathways at both 0.1 mg/L and 100 mg/L LiCl concentrations, suggesting their pivotal roles as targets for Li regulation and is independent of Li concentration. While genes related to “conjugative transfer”, “pili”, “outer membrane protein” and “antioxidant enzyme” were only significantly regulated by 100 mg/L LiCl, possible to be the specific reasons for High (100 mg/L) LiCl increased conjugative transfer frequency. This study reveals the distinct effects and mechanisms of different concentration of Li on conjugative transfer in <em>E. coli</em>, providing a theoretical basis for the understanding of the environmental effects of Li.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107263"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sublethal effects of atrazine concentrations exposure on tadpoles of Dendropsophus minutus: Evaluation of redox status, micronuclei frequencies and comet assay as biomarkers","authors":"Alice Tâmara de Carvalho Lopes ,&nbsp;Marcelino Benvindo-Souza ,&nbsp;Daiany Folador Sotero ,&nbsp;Thays Millena Alves Pedroso ,&nbsp;Acácio Arlem Tomaz ,&nbsp;Andreya Gonçalves Costa ,&nbsp;Antônia Regina dos Santos Gois ,&nbsp;Thiago Bernardi Vieira ,&nbsp;Rogério Pereira Bastos ,&nbsp;Daniela de Melo e Silva","doi":"10.1016/j.aquatox.2025.107260","DOIUrl":"10.1016/j.aquatox.2025.107260","url":null,"abstract":"<div><div>Atrazine (ATZ) is an herbicide that can persist in terrestrial and aquatic environments and potentially cause significant harm to amphibian health. Therefore, the Brazilian National Environment Council (CONAMA) sets the limit concentration of ATZ in waters at 2μg/L. Our study evaluated the genotoxic, mutagenic, and biochemical alterations in <em>Dendropsophus minutus</em> tadpoles in the 25 Gosner stage, to acute exposure (96h) of ATZ (T1 - 0.02µg/L, T2 - 0.04µg/L, T3 - 0.08µg/L, T4 - 2µg/L). The comet assay showed all concentrations caused DNA damage with an increase to T2, T3, and T4. In the micronucleus test (MN) and Erythrocyte Nuclear Abnormality test (ENA), T3 and T4 accumulated more anucleated (AN), binucleated cells (BC) and ENAs. Redox imbalance was not detected. Therefore, we conclude that the concentrations tested are not safe for the health and development of <em>D. minutus</em> tadpoles, and the CONAMA limit needs to be reviewed since all tadpoles presented DNA damage. More studies are necessary to identify other alterations that ATZ can cause in the tadpole health of tropical species. Therefore, implementing public policies aimed at safeguarding the lives of both adult and juvenile amphibians is imperative for the conservation of biodiversity and ecosystem stability.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107260"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonality of cyanobacteria and eukaryotes in Lake Geneva and the impacts of cyanotoxins on growth of the model ciliate Tetrahymena pyriformis","authors":"Niveen Ismail ,&nbsp;Paul Seguin ,&nbsp;Lola Pricam ,&nbsp;Elisabeth M.L. Janssen ,&nbsp;Tamar Kohn ,&nbsp;Bas W. Ibelings ,&nbsp;Anna Carratalà","doi":"10.1016/j.aquatox.2025.107262","DOIUrl":"10.1016/j.aquatox.2025.107262","url":null,"abstract":"<div><div>Toxic cyanobacteria are likely to be favored by global warming and other human impacts, posing significant threats to aquatic ecosystems. While cyanobacterial blooms in eutrophic lakes are widely investigated, the dynamics of cyanobacteria and the effects of their toxins and bioactive metabolites on the plankton communities in mesotrophic and oligotrophic lakes are less well understood. Here we investigated seasonal dynamics of cyanobacteria, eukaryotic algae and cyanotoxins in oligo-mesotrophic Lake Geneva—the largest and deepest lake in western Europe. High-throughput sequencing of the 16S rRNA genes in 143 samples along a water column revealed that Lake Geneva hosts diverse, co-dominant cyanobacterial genera, including <em>Planktothrix, Cyanobium, Pseudanabaena</em>, and <em>Aphanizomenon.</em> The abundance of the <em>mcyA</em> gene marker for microcystin production was highly correlated with total cyanobacteria abundance, obtained from qPCR of the 16S rRNA genes. Targeted LC-HRMS/MS analysis demonstrated peak concentrations of cyanotoxins in September and December 2021 at the deep chlorophyll-a maximum layer, reaching up to 1474 ng/l for anabaenopeptins and 144 ng/l for microcystins. The toxin peaks did not correlate with the abundance or variations in the cyanobacteria or eukaryote community, but they were correlated in time with seasonal lows in the abundances of ciliates (18S rRNA analysis). Laboratory exposure tests demonstrated that growth of the model ciliate <em>Tetrahymena pyriformis</em> was inhibited by Microcystin-RR and Anabaenopeptin A at environmentally relevant concentrations in the ng/l-range, in natural lake water, synthetic freshwater, and growth media spiked with the cyanotoxins. Our findings suggest that even low concentrations (in the ng/l-range) of microcystins and anabaenopeptins, reduce growth of ciliates such as <em>T. pyriformis</em> and can be expected to have wider impacts on the eukaryote communities.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107262"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional modulation in Mediterranean Mussel Mytilus galloprovincialis following exposure to four pharmaceuticals widely distributed in coastal areas","authors":"Ilaria Bernardini ,&nbsp;Marica Mezzelani ,&nbsp;Michela Panni ,&nbsp;Giulia Dalla Rovere ,&nbsp;Alessandro Nardi ,&nbsp;Ouafa El Idrissi ,&nbsp;Luca Peruzza ,&nbsp;Stefania Gorbi ,&nbsp;Serena Ferraresso ,&nbsp;Luca Bargelloni ,&nbsp;Tomaso Patarnello ,&nbsp;Francesco Regoli ,&nbsp;Massimo Milan","doi":"10.1016/j.aquatox.2025.107255","DOIUrl":"10.1016/j.aquatox.2025.107255","url":null,"abstract":"<div><div>Ecotoxicological risk and the mode of action of human drugs on non-target marine animals remain unclear, keeping a gap of knowledge on risks related to ecosystem disruption and chemical contamination of food chains.</div><div>Understanding these impacts is critical to developing proper waste management practices and regulatory frameworks to prevent long-term environmental and human health problems. This study investigates the impacts of Gemfibrozil, Metformin, Ramipril, and Venlafaxine, individually and combined on <em>Mytilus galloprovincialis</em> over 30 days and assesses persistent effects post-recovery using RNA-seq and 16S rRNA microbiota profiling. All pharmaceuticals caused few changes in the microbiota while gene expression analyses highlighted drug-specific alterations. Gemfibrozil exposure led to alterations in lipid and fatty acid metabolism, suggesting a similar mode of action to that observed in target species. Metformin significantly impacted the mussels' energy metabolism, with disruptions in specific genes and pathways potentially related to glucose uptake and insulin signaling. Metformin was also the treatment leading to the most significant changes in predicted functional profiles of the microbiota, suggesting that it may influence the microbiota's potential to interact with host glucose metabolism. Ramipril exposure resulted in the up-regulation of stress response and cell cycle regulation pathways and Venlafaxine induced changes in serotonin and synapse pathways, indicating potential similarities in mechanisms of action with target species. Mixture of the four pharmaceuticals severely impacted mussel physiology, including impairment of oxidative phosphorylation and compensatory activation of several pathways involved in energy metabolism. Despite recovery after depuration, changes in stress and energy related metabolism pathways suggests potential persistent effects from combined pharmaceutical exposure. Notably, the up-regulation of mTOR1 signaling in all treatments after 30 days underscores its key role in coordinating bivalve stress responses. The Transcriptomic Hazard Index (THI) calculated for each treatment indicates major/severe hazards after exposure that decreased to slight/moderate hazards after depuration.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107255"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical behavior and ecological risk of biofilm-mediated microplastics in aquatic environments","authors":"Ranran Zhou ,&nbsp;Xirong Huang ,&nbsp;Yongtao Ni,&nbsp;Zewei Ma,&nbsp;Hengchen Wei,&nbsp;Qijie Jin,&nbsp;Zhuhong Ding","doi":"10.1016/j.aquatox.2024.107209","DOIUrl":"10.1016/j.aquatox.2024.107209","url":null,"abstract":"<div><div>The prevalence of microplastics (MPs) in aquatic environments has become the core of environmental pollution. In recent years, the inevitable biological aging process of MPs in natural environments has attracted researchers’ attention. Such biofilm-mediated MPs, colonized by microorganisms, affect the physicochemical behavior and potential ecological risks of MPs. Therefore, it is critical to understand the impact of MPs’ biofilm formation on the environmental fate and toxicity of MPs. This review presented a comprehensive discussion of the impact of biofilm formation on unique carrier effects and toxicological effects of MPs in aquatic environments. First, the biofilm formation process on MPs, the compositions of microorganisms in biofilm and the factors influencing biofilm formation were briefly summarized. Second, the sorption of pollutants and enrichment of antibiotic resistance genes onto biofilm-mediated MPs were discussed. Third, the potential effects of biofilm-mediated MPs on gut microbiota were analyzed. Finally, gaps in the field that require further investigations were put forward. This review emphasized that biofilm-mediated MPs have higher environmental risks and ecotoxicity, which is helpful in providing new insights for pollution prevention and control of new pollutant MPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107209"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-comprehensive approach to assess the responses of the Mediterranean mussel Mytilus galloprovincialis (Lamarck, 1919) to a simulation of a diesel-oil mixture spill","authors":"F. Bertini ,&nbsp;V. Catania ,&nbsp;L. Scirè Calabrisotto ,&nbsp;M. Dara ,&nbsp;L. Bisanti ,&nbsp;C. La Corte ,&nbsp;M. Staropoli ,&nbsp;D. Piazzese ,&nbsp;M.G. Parisi ,&nbsp;D. Parrinello ,&nbsp;M. Cammarata","doi":"10.1016/j.aquatox.2024.107188","DOIUrl":"10.1016/j.aquatox.2024.107188","url":null,"abstract":"<div><div>Oil spills are a major cause of pollution impacting marine ecosystems. In this work, the effects of short-term exposure to three different concentrations of a hydrocarbon mixture (HC), that simulated the action of such an event, were investigated on <em>Mytilus galloprovincialis</em> specimens. Physiological effects were measured using a battery of biomarkers consisting of cellular activity (phagocytosis), immune-related enzymes, chaperonins (HSP70 and HSC70), and histomorphological alterations. Different concentrations of HC led to a significant decrease in phagocytosis, especially following high concentrations. Immune-related enzymes evaluated in hemolymph and digestive gland extract showed up-regulation, suggesting the activation of antioxidant, detoxicant, and inflammatory responses. Morphological alterations of digestive gland tubules were observed after exposure to the HC. HSP70 and HSC70 activity was up regulated following the treatments, indicating their involvement in maintaining organism homeostasis. In addition, the diversity and composition of hemolymph and digestive gland microbiota exposed to HC were analyzed by automated ribosomal intergenic spacer analysis (ARISA) and a Next Generation Sequencing (NGS) approach to evaluate the connection with hydrocarbon contamination. Metagenomic analysis revealed significant differences in the hemolymph and digestive gland microbiota composition between mussels exposed and unexposed to HC. Exposure to increasing HC concentrations had a positive effect on microbial diversity with clear adaptative responses, and an increase in the relative abundance of several known degrading bacterial genera, including <em>Alcanivorax, Roseovarius, Pseudomonas, Vibrio, Oleibacter</em>. These results show the utility of a multi-comprehensive approach to evaluating functional adaptation in terms of immunological dysfunctions and microbiota alteration in the sentinel organism <em>M. galloprovincialis</em>.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107188"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}