Aquatic Toxicology最新文献

{"title":"Unveiling the mechanisms of reproductive toxicity induced by full life-cycle exposure to environmentally relevant concentrations of tris(2-chloroethyl) phosphate in male zebrafish","authors":"Hongkai Wang ,&nbsp;Jieyu Ding ,&nbsp;Shiyi Luo ,&nbsp;Meijiao Yan ,&nbsp;Fengxiao Hu","doi":"10.1016/j.aquatox.2024.107079","DOIUrl":"10.1016/j.aquatox.2024.107079","url":null,"abstract":"<div><p>Tris (2-chloroethyl) phosphate (TCEP), a commonly used organophosphate flame retardant, has garnered considerable concern owing to its pervasive presence in the environment and its toxic effects on living organisms. The perpetuation of populations and species hinges on successful reproduction, yet research into the mechanisms underlying reproductive toxicity remains scant, particularly in aquatic species. In this work, zebrafish embryos were exposed to TCEP (0, 0.8, 4, 20, and 100 µg/L) for 120 days until sexual maturation, and multiple reproductive endpoints were investigated in male zebrafish. Our results showed that the body weight, body length, and gonadal-somatic index (GSI) were remarkably decreased in all TCEP treatment groups (except GSI in the 0.8 µg/L TCEP-treated group). Long-term exposure to TCEP led to reduced reproductive capacity of male zebrafish, as evidenced by decreased fertilization. Histological observation gave an indication of delayed testicular development and inhibited spermatogenesis under TCEP stress. The content of testosterone (T) was significantly elevated in all TCEP treatment group, whereas 17 β-estradiol (E2) levels remained stable. Transcriptome analysis revealed a lot of downregulated genes involved in steroid hormone biosynthesis, energy metabolism, and sperm motility, which might account for the imbalance of steroid hormone levels, retarded spermatogenesis and declined fertilization success. Overall, these findings offered a thorough understanding of the mechanisms underlying the male reproductive toxicity caused by TCEP, highlight the risk of TCEP on reproductive health of fish.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107079"},"PeriodicalIF":4.1,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of inorganic mercury on carotenoids in freshwater algae: Insights from single-cell resonance Raman spectroscopy","authors":"Ion Nesterovschi ,&nbsp;Karlo Maškarić ,&nbsp;Iuliana-Cornelia Poplăcean ,&nbsp;João P. Santos ,&nbsp;Arin Kantarciyan ,&nbsp;Vera I. Slaveykova ,&nbsp;Simona Cîntă Pînzaru","doi":"10.1016/j.aquatox.2024.107085","DOIUrl":"10.1016/j.aquatox.2024.107085","url":null,"abstract":"<div><p>The influence of inorganic mercury (Hg(II)) exposure on photosynthetic microorganisms and their pigments remains understudied. Here, we employed resonance Raman (RR) spectroscopy to investigate the responses of two freshwater phytoplankton species, the green alga <em>Chlamydomonas reinhardtii</em> and the diatom <em>Cyclotella meneghiniana</em> to Hg(II) exposure. We selectively recorded the spectral RR signature of carotenoids in intact cells exposed to concentrations of 10 nM and 100 nM of Hg(II), representative for contaminated environment and unexposed control cells. A two-hour exposure of <em>C. reinhardtii</em> resulted in a slight decrease in lutein and β-carotene levels, while total carotenoids RR band broadening, as revealed by the FWHM of the υ₁(C=C) stretching mode from averaged RR spectra, suggested conformational changes in pigments. Higher Hg(II) concentration induced more pronounced conformational changes. Similarly, a two-hour exposure of <em>C. meneghiniana</em> resulted in slight decreased level of the fucoxanthin, while diadinoxanthin showed an opposite trend compared to control<strong>:</strong> when fucoxanthin decreased, diadinoxanthin increased under 10 nM Hg (II) exposure. At higher concentrations, the decrease in fucoxanthin was less pronounced, accompanied by a broadening of the band area, (with FHHM increased), indicating possible conformer occurrence in response to Hg-induced stress. The changes in the main carotenoid species of the two algae are species-specific, Hg(II) concentration-specific, and dependent on exposure time. The calculated spectral differences in absorbances from UV–VIS spectra of methanol extracts from each group supported the main findings obtained by RR, though with caution due to the selective extraction efficiency of the respective carotenoids. This study highlighted for a first time the capability of single-cell RR spectroscopy as a valuable tool for toxicity assessment and for comprehending early-stage alterations in carotenoid metabolism due to toxic metal exposure in vivo.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107085"},"PeriodicalIF":4.1,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0166445X24002558/pdfft?md5=52a0dc12b73f050adf7d2bae080680b2&pid=1-s2.0-S0166445X24002558-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hexafluoropropylene oxide trimer acid (HFPO-TA) disrupts sex differentiation of zebrafish (Danio rerio) via an epigenetic mechanism of DNA methylation","authors":"Dan Yang,&nbsp;Fanghua Li,&nbsp;Xiaohui Zhao,&nbsp;Shasha Dong,&nbsp;Guobin Song,&nbsp;Haonan Wang,&nbsp;Xiaoying Li,&nbsp;Guanghui Ding","doi":"10.1016/j.aquatox.2024.107077","DOIUrl":"10.1016/j.aquatox.2024.107077","url":null,"abstract":"<div><p>Hexafluoropropylene oxide trimer acid (HFPO-TA), an alternative to perfluorooctanoic acid, has been shown to have estrogenic effects. However, its potential to disrupt fish sex differentiation during gonadal development remains unknown. Therefore, this study exposed zebrafish to HFPO-TA from approximately 2 hours post fertilization (hpf) to 60 days post fertilization (dpf) to investigate its effects on sex differentiation. Results indicated that HFPO-TA disrupted steroid hormone homeostasis, delayed gonadal development in both sexes, and resulted in a female-skewed sex ratio in zebrafish. HFPO-TA exposure up-regulated gene expressions of <em>cyp19a1a, esr1, vtg1</em> and <em>foxl2</em>, while down-regulated those of <em>amh, sox9a</em> and <em>dmrt1</em>. These suggested that HFPO-TA dysregulated the expressions of key genes related to sex differentiation of zebrafish, promoted the production and activation of estrogen, and further induced the feminization. Interestingly, we observed promoter hypomethylation of <em>cyp19a1a</em> and promoter hypermethylation of <em>amh</em> in male zebrafish, which were negatively associated with their gene expressions. These suggested that HFPO-TA dysregulated these key genes through DNA methylation in their promoters. Therefore, the HFPO-TA disrupted the sex differentiation of zebrafish through an epigenetic mechanism involving DNA methylation, ultimately skewing the sex ratio towards females. Overall, this study demonstrated adverse effects of HFPO-TA on fish sex differentiation and provided novel insights into the underlying epigenetic mechanism.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"275 ","pages":"Article 107077"},"PeriodicalIF":4.1,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142136075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-lethal biomarkers as promising tools for fish health assessment: In situ exposure to bifenthrin as a case study","authors":"Carla Bacchetta ,&nbsp;Jimena Cazenave ,&nbsp;Celeste Mora ,&nbsp;Melina P. Michlig ,&nbsp;María R. Repetti ,&nbsp;Andrea S. Rossi","doi":"10.1016/j.aquatox.2024.107083","DOIUrl":"10.1016/j.aquatox.2024.107083","url":null,"abstract":"<div><p>Non-lethal biomonitoring should provide an innovative approach to establish bioethical protocols for the management of both aquaculture and wild fisheries resources. We aimed to assess non-lethal biomarkers in <em>Piaractus mesopotamicus</em> caged in a rice field during a bifenthrin (BF) application. We analyzed parameters related to the immune system, energy metabolism and oxidative stress in fish skin mucus and blood plasma. Fish exposed to BF showed a significant increase in skin mucus glucose levels and the enzymatic activities of protease, alkaline phosphatase and superoxide dismutase. Regarding plasmatic parameters, BF increased the levels of glucose, total protein and albumin, but decreased triglycerides. In addition, increased activities of lysozyme and alkaline phosphatase were found in the blood plasma of exposed fish. Our results indicated an increased energy demand, altered immune function and a mild oxidative stress response in fish exposed <em>in situ</em> to BF. We have shown that skin mucus and blood plasma are very promising matrices for the development of non-lethal biomarkers to assess fish health in a stressed environment.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107083"},"PeriodicalIF":4.1,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of long-term fluoxetine exposure on morphology, but not behaviour or metabolic rate, in male guppies (Poecilia reticulata)","authors":"Kate N. Fergusson ,&nbsp;James L. Tanner ,&nbsp;Jack A. Brand ,&nbsp;Stephanie L. Hannington ,&nbsp;Amanda K. Pettersen ,&nbsp;Josefin Sundin ,&nbsp;Minna Saaristo ,&nbsp;Michael G. Bertram ,&nbsp;Jake M. Martin ,&nbsp;Bob B.M. Wong","doi":"10.1016/j.aquatox.2024.107082","DOIUrl":"10.1016/j.aquatox.2024.107082","url":null,"abstract":"<div><p>Contamination of aquatic ecosystems by pharmaceuticals is a growing threat worldwide. The antidepressant fluoxetine is one such pharmaceutical that is frequently detected in aquatic ecosystems, and has been found to alter the behaviour and physiology of exposed wildlife. Few studies, however, have investigated potential combined effects on behaviour and metabolic rate. In addition, exposures are often short in duration and rarely conducted under ecologically relevant conditions. Here, we examined the impacts of long-term fluoxetine exposure on boldness (exploration, activity, and antipredator behaviour), metabolic rate, and morphology in male guppies (<em>Poecilia reticulata</em>). Specifically, fish were exposed for 8 months (corresponding to approximately two overlapping generations) in semi-natural mesocosms to one of three treatments: an unexposed control (0 ng L⁻¹), or low or high fluoxetine (mean measured concentrations: 30 ng L⁻¹ and 292 ng L⁻¹, respectively). Following exposure, we quantified male exploratory behaviour and activity in a novel environment (maze arena) and antipredator behaviour in the presence or absence of a live predator (spangled perch, <em>Leiopotherapon unicolor</em>), as well as metabolic rate and morphology (mass, standard length, and scaled mass index). Fluoxetine exposure did not significantly alter boldness, metabolic rate, mass, or standard length. However, fluoxetine exposure did alter body condition, whereby fish in the high treatment had a higher scaled mass index than control fish. Our results, considered alongside previous work, underscore the importance of exposure duration in mediating the effects of fluoxetine on fitness-related traits. Continued research under extended exposure periods (i.e., spanning multiple generations) is essential if we are to accurately predict the ecological impacts of fluoxetine on exposed wildlife, and their underlying mechanism(s).</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107082"},"PeriodicalIF":4.1,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0166445X24002522/pdfft?md5=a5cd7cd67b1bbbb34dddbd26abaa7dcf&pid=1-s2.0-S0166445X24002522-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How relevant are sterols in the mode of action of prymnesins?","authors":"Hélène-Christine Prause ,&nbsp;Deniz Berk ,&nbsp;Catharina Alves-de-Souza ,&nbsp;Per J. Hansen ,&nbsp;Thomas O. Larsen ,&nbsp;Doris Marko ,&nbsp;Giorgia Del Favero ,&nbsp;Allen Place ,&nbsp;Elisabeth Varga","doi":"10.1016/j.aquatox.2024.107080","DOIUrl":"10.1016/j.aquatox.2024.107080","url":null,"abstract":"<div><p>Prymnesins, produced by the haptophyte <em>Prymnesium parvum</em>, are considered responsible for fish kills when this species blooms. Although their toxic mechanism is not fully understood, membrane disruptive properties have been ascribed to A-type prymnesins. Currently it is suggested that pore-formation is the underlying cause of cell disruption. Here the hypothesis that A-, B-, and C-type prymnesins interact with sterols in order to create pores was tested. Prymnesin mixtures containing various analogs of the same type were applied in hemolysis and cytotoxicity assays using Atlantic salmon <em>Salmo salar</em> erythrocytes or rainbow trout RTgill-W1 cells. The hemolytic potency of the prymnesin types reflected their cytotoxic potential, with approximate concentrations reaching 50 % hemolysis (HC₅₀) of 4 nM (A-type), 54 nM (C-type), and 600 nM (B-type). Variabilities in prymnesin profiles were shown to influence potency. Prymnesin-A (3 Cl) + 2 pentose + hexose was likely responsible for the strong toxicity of A-type samples. Co-incubation with cholesterol and epi-cholesterol pre-hemolysis reduced the potential by about 50 % irrespective of sterol concentration, suggesting interactions with sterols. However, this effect was not observed in RTgill-W1 toxicity. Treatment of RTgill-W1 cells with 10 µM lovastatin or 10 µM methyl-β-cyclodextrin-cholesterol modified cholesterol levels by 20-30 %. Regardless, prymnesin cytotoxicity remained unaltered in the modified cells. SPR data showed that B-type prymnesins likely bound with a single exponential decay while A-types seemed to have a more complex binding. Overall, interaction with cholesterol appeared to play only a partial role in the cytotoxic mechanism of pore-formation. It is suggested that prymnesins initially interact with cholesterol and stabilize pores through a subsequent, still unknown mechanism possibly including other membrane lipids or proteins.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107080"},"PeriodicalIF":4.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142171769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-conventional endpoints show higher sulfoxaflor toxicity to Chironomus riparius than conventional endpoints in a multistress environment","authors":"Sofie B. Rasmussen ,&nbsp;Thijs Bosker ,&nbsp;S．Henrik Barmentlo ,&nbsp;Olof Berglund ,&nbsp;Martina G. Vijver","doi":"10.1016/j.aquatox.2024.107074","DOIUrl":"10.1016/j.aquatox.2024.107074","url":null,"abstract":"<div><p>Evidence grows that standard toxicity testing might underestimate the environmental risk of neurotoxic insecticides. Behavioural endpoints such as locomotion and mobility have been suggested as sensitive and ecologically relevant additions to the standard tested endpoints. Possible interactive effects of chemicals and additional stressors are typically overlooked in standardised testing. Therefore, we aimed to investigate how concurrent exposure to environmental stressors (increased temperature and predation cues) and a nicotinic acetylcholine receptor (nAChR)-modulating insecticide (‘sulfoxaflor’) impact <em>Chironomus riparius</em> across a range of conventional and non-conventional endpoints. We used a multifactorial experimental design encompassing three stressors, sulfoxaflor (2.0–110 µg/L), predation risk (presence/absence of predatory cues), and elevated temperature (20 °C and 23 °C), yielding a total of 24 distinct treatment conditions. Additional stressors did not change the sensitivity of <em>C. riparius</em> to sulfoxaflor. To assess potential additive effects, we applied an Independent Action (IA) model to predict the impact on eight endpoints, including conventional endpoints (growth, survival, total emergence, and emergence time) and less conventional endpoints (the size of the adults, swimming abilities and exploration behaviour). For the conventional endpoints, observed effects were either lower than expected or well-predicted by the IA model. In contrast, we found greater than predicted effects of predation cues and temperature in combination with sulfoxaflor on adult size, larval exploration, and swimming behaviour. However, in contrast to the non-conventional endpoints, no conventional endpoints detected interactive effects of the neurotoxic insecticide and the environmental stressors. Acknowledging these interactions, increasing ecological context of ecotoxicological test systems may, therefore, advance environmental risk analysis and interpretation as the safe environmental concentrations of neurotoxic insecticides depend on the context of both the test organism and its environment.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"275 ","pages":"Article 107074"},"PeriodicalIF":4.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0166445X24002443/pdfft?md5=e6ece69472f78e5a89f3aec49fedb0bf&pid=1-s2.0-S0166445X24002443-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142143966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of aflatoxin B1 and sterigmatocystin on DNA repair genes in common carp","authors":"Rubina Tünde Szabó ,&nbsp;Mária Kovács-Weber ,&nbsp;Krisztián Milán Balogh ,&nbsp;Miklós Mézes ,&nbsp;Balázs Kovács","doi":"10.1016/j.aquatox.2024.107076","DOIUrl":"10.1016/j.aquatox.2024.107076","url":null,"abstract":"<div><p>The present study aimed to investigate the short-time (24 h) effect of aflatoxin B1 (AFB1) and sterigmatocystin (STC) on the expression of <em>hsp70, p53, gadd45</em>, and <em>ogg1</em> genes in common carp hepatopancreas. Our results showed that aflatoxin B1 and sterigmatocystin can stimulate the expression of DNA repair genes, mainly by hour 24. This significant finding contributes to our understanding of the short-term effects of these mycotoxins on <em>ogg1</em> genes in common carp hepatopancreas. One-year-old common carp juveniles were randomly distributed into five groups (Control, AFB1 0.4 mg kg⁻¹ feed, STC1 1 mg kg⁻¹ feed, STC2 2 mg kg⁻¹ feed, and STC3 3 mg kg⁻¹ feed). Hepatopancreas samples were collected three times (8, 16, and 24 h) in each group. No significant ogg1 and <em>p53</em> expression changes were observed at 8 and 16 h after exposure. All measured genes were upregulated by the 24th hour in aflatoxin and STC3 groups. An increase in <em>hsp70</em> gene expression was detected in all groups and all sampling. A significant decrease in <em>gadd45aa</em> gene expression was observed in the aflatoxin B1 group at hour 8. At hour 16, there was no significant change, while at hour 24, all treated groups were significantly different from the control. In summary, our results suggest that aflatoxin B1 and sterigmatocystin can stimulate the expression of DNA repair genes, mainly by hour 24. Further investigations are needed to get information about DNA damage parallel to the DNA repair mechanisms.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107076"},"PeriodicalIF":4.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0166445X24002467/pdfft?md5=adfb559ccde41467af2baf967c796a06&pid=1-s2.0-S0166445X24002467-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combinatorial accumulation, stress response, detoxification and synaptic transmission effects of cadmium and selenium in clams Ruditapes philippinarum","authors":"Xiaoyu Zhang ,&nbsp;Tao Sun ,&nbsp;Fei Li ,&nbsp;Chenglong Ji ,&nbsp;Hongmei Liu ,&nbsp;Huifeng Wu","doi":"10.1016/j.aquatox.2024.107075","DOIUrl":"10.1016/j.aquatox.2024.107075","url":null,"abstract":"<div><p>This study investigated the toxicological effects and mechanisms of cadmium (Cd) (5 and 50 μg/L) and selenium (Se) (3 and 30 μg/L) at environmentally relevant concentrations on the gills and digestive glands of clams <em>Ruditapes philippinarum</em>. Results indicated that Cd and Se could tissue-specifically impact osmoregulation, energy metabolism, and synaptic transmission in the gills and digestive glands of clams. After exposure to 50 μg/L Cd, the digestive glands of clams up-regulated the expression of <em>methionine-gamma-lyase</em> and <em>metallothionein</em> for detoxification. Clam digestive glands exposed to 3 μg/L Se up-regulated the expression of <em>catalase</em> and <em>glutathione peroxidase</em> to alleviate oxidative stress, and down-regulated the expression of <em>selenide-water dikinase</em> to reduce the conversion of inorganic Se. Additionally, the interaction mode between Cd and Se largely depended on their molar ratio, with a ratio of 11.71 (50 μg/L Cd + 3 μg/L Se) demonstrated to be particularly harmful, as manifested by significantly more lesions, oxidative stress, and detoxification demand in clams than those exposed to Cd or Se alone. Collectively, this study revealed the complex interaction patterns and mechanisms of Cd and Se on clams, providing a reference for exploring their single and combined toxicity.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"275 ","pages":"Article 107075"},"PeriodicalIF":4.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142143937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-MS untargeted metabolomics reveals metabolic disturbance and ferroptosis in MWCNTs-induced hepatotoxicity of Cyprinus carpio","authors":"Xiaochan Gao,&nbsp;Yimin Li,&nbsp;Jiaqi Shen,&nbsp;Yong Huang,&nbsp;Yashuai Wang,&nbsp;Xuehan Niu","doi":"10.1016/j.aquatox.2024.107078","DOIUrl":"10.1016/j.aquatox.2024.107078","url":null,"abstract":"<div><p>In recent years, there is a great concern about the potential adverse effects of carbon nanotubes (CNTs) on the aquatic systems due to their increasingly extensive application. In this study, juvenile <em>Cyprinus carpio</em> were exposed to multi-walled CNTs (MWCNTs) at concentrations of 0, 0.25, and 2.5 mg L^-1 for 28 days. Then, oxidative stress indicators and metabolite profile of the livers were assessed. Results showed the significant increase of malondialdehyde (MDA) content and decrease of glutathione (GSH) activities in fish treated with 2.5 mg L^-1 MWCNTs. LC-MS untargeted metabolomics demonstrated that 406 and 274 metabolites in fish treated with 2.5 mg L^-1 MWCNTs were significantly up- and down-regulated, respectively. KEGG functional annotation analysis showed the disturbance of amino acid metabolism, lipid metabolism, and nucleotide metabolism. In addition, ferroptosis signaling pathway was detected. Therefore, iron content analysis and quantitative real-time RT-PCR assay were performed furtherly to validate the contribution of ferroptosis to MWCNTs-induced hepatotoxicity. The iron content increased significantly and the mRNA levels of ferroptosis-related genes including STEAP3, ACSL4, NCOA4, TFR1, NRF2, SLC3A2, SLC7A11, GPX4, and FPN1 were also obviously changed. Taken together, our study suggested that MWCNTs exposure-induced ferroptosis were associated with iron overload and lipid peroxidation via NRF2/SLC7A11/GSH/GPX4 axis. Our findings provide essential information to understand the mechanism of CNTs-induced hepatotoxicity in fish and explore potential biomarkers.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"275 ","pages":"Article 107078"},"PeriodicalIF":4.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142143969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}