BPA破坏甲壳类动物繁殖：日本沼虾的组织特异性积累、氧化应激和基因失调

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-08-11 DOI:10.1016/j.aquatox.2025.107538

Jintao Liu , Ruijie Zhu , Shuhao Wang , Xinyuan Dai , Anran Zou , Fengjuan Jiang , Shengming Sun , Jiangtao Ou , Weihong Zhao

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引用次数: 0

摘要

双酚A （BPA）是一种普遍存在的环境内分泌干扰物，由于其生殖毒性，对水生生态系统的威胁越来越大。本研究通过分析BPA在日本沼虾（Macrobrachium nipponense）的组织特异性生物积累（卵巢、肝胰腺、受精卵）、抗氧化反应和发育基因调控来研究BPA是如何破坏卵巢发育的。实验结果表明，暴露于10、100和1000 μg/L BPA 10天后，卵巢和肝胰脏均有显著的BPA积累，而受精卵仅在最高浓度（1000 μg/L）时才有显著的BPA积累(P <；0.05)。卵巢谷胱甘肽过氧化物酶（GSH-Px）活性呈浓度依赖性增加，而受精卵呈相反模式。基因表达分析显示，卵巢和肝胰腺细胞色素P450 49A1 （CYP49a1）在BPA浓度最高时达到峰值，组织蛋白酶D （CTSD）和过氧化氢酶（CAT）在中间浓度（100 μg/L）时达到峰值(P <；暴露于BPA后，卵巢内雌性不育同型体（fsh）保持稳定，但肝胰腺内的fsh显著降低(P <；0.05)。这些结果提供了初步证据，表明BPA可能通过干扰抗氧化系统和改变关键发育基因的表达来破坏日本蛤的卵巢发育，突出了它在这种重要的商业甲壳类动物中潜在的内分泌干扰作用。

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BPA disrupts crustacean reproduction: Tissue-specific accumulation, oxidative stress, and gene dysregulation in Macrobrachium nipponense

Bisphenol A (BPA), a pervasive environmental endocrine disruptor, increasingly threatens aquatic ecosystems due to its reproductive toxicity. This study investigates how BPA disrupts ovarian development in Macrobrachium nipponense - by analyzing its tissue-specific bioaccumulation (ovaries, hepatopancreas, fertilized eggs), antioxidant responses, and developmental gene regulation. Experimental results demonstrated that after 10-day exposure to 10, 100, and 1000 μg/L BPA, significant BPA accumulation was observed in both ovaries and hepatopancreas, while fertilized eggs showed notable accumulation only at the highest concentration (1000 μg/L) (P < 0.05). The ovarian glutathione peroxidase (GSH-Px) activity displayed a concentration-dependent increase, contrasting with the inverse pattern observed in fertilized eggs. Gene expression analysis revealed that cytochrome P450 49A1 (CYP49a1) in both ovaries and hepatopancreas reached peak levels at the highest BPA concentration, whereas cathepsin D (CTSD) and catalase (CAT) expression peaked at the intermediate concentration (100 μg/L) in both tissues (P < 0.05), while female sterile homeotic (fsh) in ovaries remained steady but significantly decreased in hepatopancreas upon BPA exposure (P < 0.05). These results provide preliminary evidence that BPA may disrupt ovarian development in M. nipponense through interfering with antioxidant systems and altering the expression of key developmental genes, highlighting its potential endocrine-disrupting effects in this commercially important crustacean species.

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.