Aquatic Toxicology最新文献

{"title":"Uptake, removal and trophic transfer of fluorescent polyethylene microplastics by freshwater model organisms: the impact of particle size and food availability","authors":"Ning Gong ,&nbsp;Zhiyuan Wang ,&nbsp;Xiaofan Wang ,&nbsp;Kuishuang Shao","doi":"10.1016/j.aquatox.2024.107165","DOIUrl":"10.1016/j.aquatox.2024.107165","url":null,"abstract":"<div><div>As an emerging contaminant, microplastics (MPs) are widely distributed in freshwater ecosystems and pose potential threats to aquatic organisms, attracting significant attention from both the scientific community and the general public. However, there is still uncertainty regarding the mechanisms of MPs transfer within aquatic biota and how particle size and food availability influence their transport patterns. In this study, zebrafish (<em>Danio rerio</em>) were selected as a model organism to investigate the uptake and elimination of fluorescent polyethylene (PE) MPs under different exposure scenarios (waterborne or trophic transfer, with or without food) and varying particle sizes (ranging from 10-300 μm at concentrations of 0.1, 2, and 300 mg/L). Additionally, water fleas (<em>Daphnia magna</em>) were provided as prey for the fish. The dynamic accumulation of PE-MPs sized between 10-20 μm at a concentration of 25 mg/L by daphnia was also determined along with its impact on animal feeding behavior. The results demonstrated that both organisms were capable of ingesting PE-MPs during exposures lasting up to 24 hours for daphnia and up to 72 hours for zebrafish. Furthermore, rapid elimination rates were observed within just 30 minutes for daphnia and between 6-12 hours for zebrafish. The presence of food reduced MPs uptake and removal by daphnia but significantly increased MP elimination by fish. Zebrafish showed a preference for ingesting larger-sized MPs that they could easily recognize; however, trophic transfer from daphnia to fish was found to be the primary route of ingestion specifically for PE-MPs sized between 10-20 μm. The findings suggest that while fish directly ingest fewer invisible MPs from the water column, they still accumulate these particles through predation on contaminated prey organisms. Therefore, it is imperative to prioritize the ecological risks associated with the transfer of MPs from zooplankton to fish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"Article 107165"},"PeriodicalIF":4.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiological, energy metabolism, and metabonomic responses indicate the stress response in the hepatopancreas of Litopenaeus vannamei to nitrite stress.","authors":"Yafei Duan, Guowei Zhong, Meng Xiao, Yukai Yang, Yun Wang, Yuxiu Nan","doi":"10.1016/j.aquatox.2024.107164","DOIUrl":"https://doi.org/10.1016/j.aquatox.2024.107164","url":null,"abstract":"<p><p>Nitrite is a toxic substance found in rearing water that affects shrimp health. The hepatopancreas is an important digestive, immune, and metabolic organ in the shrimp. In this study, shrimps (Litopenaeus vannamei) were separately exposed to 1 and 5 mg/L nitrite stress for 48 h, and the toxicity of nitrite in the hepatopancreas was explored by integrating histology, physiological indicators, energy metabolism, and metabolomics. Nitrite stress induced morphological changes and stress responses in the hepatopancreas. Specifically, physiology-related indices, such as the relative gene expression levels of antioxidants (ROMO1, Nrf2, GPx), endoplasmic reticulum stress (Bip, IRE1 and XBP1), and immune genes (ALF, Pen-3, Lys) were decreased, whereas the gene expression of apoptosis (Casp-3), detoxification (CYP450), and glutamic oxaloacetic transaminase (GOT) activity were increased. The activities of osmotic adjustment-related enzymes (NKA, CMA, and ATPase) also decreased. Energy metabolism-related indices, such as pyruvate and hepatic glycogen contents, increased, whereas glucose, lactic acid, triglyceride, and ATP contents and ATPase activity decreased, and the relative gene expression levels of carbohydrate metabolism (PDH, HK, and LDH) and electron-transport chain genes (CytC, COI and CCO) decreased, and the expressions of lipid metabolism (AMPK, SREBP, and FAS), tricarboxylic acid cycle (MDH, CS, IDH and FH) genes were also disturbed. The metabolic pattern of the hepatopancreas was affected by nitrite stress. Glycine, serine, and threonine metabolism were highly affected, and more functional amino acids varied in the 5 mg/L nitrite stress group. These results reveal the toxic effects of nitrite stress on the stress response, physiology, energy metabolism, and metabolite homeostasis in the hepatopancreas of shrimp. Several potential metabolite biomarker candidates were identified for toxicological evaluation.</p>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"107164"},"PeriodicalIF":4.1,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse effects of glyphosate-based herbicide on hatching rate, morphological alterations, and acetylcholinesterase (AChE) expression in golden apple snail eggs","authors":"Phochit Nanthanawat ,&nbsp;Wilaiporn Insuwan ,&nbsp;Witchuda Prasatkaew ,&nbsp;Jakkaphun Nanuam ,&nbsp;Panomsak Meemon ,&nbsp;Chutima Thanomsit","doi":"10.1016/j.aquatox.2024.107162","DOIUrl":"10.1016/j.aquatox.2024.107162","url":null,"abstract":"<div><div>This research investigated the effects of glyphosate herbicide on golden apple snail (<em>Pomacea canaliculata</em>) eggs, focusing on hatching rates, morphological changes, and acetylcholinesterase (AChE) expression to explore its potential as a biomarker of exposure. Additionally, key bioactive components in golden apple snail eggs were characterized. The study demonstrated that glyphosate toxicity increased with both exposure time and concentration. Optical coherence tomography (OCT) analysis revealed adverse morphological effects on eggs and embryos, including changes in shell structure and organ development. SDS-PAGE analysis identified four main protein bands, including Perivitellin 2 in three isoforms (98, 67, and 31 kDa) and Ovorubin (28 kDa). Lipids, phosphorus, and carbohydrates were identified as key components through Sudan Black B, Methyl Green, and Alcian Blue staining. AChE, with a molecular weight of 71 kDa, was further analyzed by Western blot, showing decreased expression with prolonged and higher concentrations of glyphosate exposure. GC–MS analysis identified major bioactive compounds in untreated eggs, including 3-Fluoro-β, 5-dihydroxy-N-methylbenzeneethanamine, 2-Aziridinylethylamine, and dextroamphetamine, which have pharmaceutical properties such as anti-hypertensive, diuretic, anti-diabetic, and anti-inflammatory effects, as well as potential applications in treating attention deficit hyperactivity disorder and narcolepsy. These compounds were present at lower levels in glyphosate-exposed groups, suggesting glyphosate's impact on the eggs' biochemical defense mechanisms. This study highlights the potential effects of glyphosate on golden apple snail eggs, which may have implications for future snail populations and aquatic ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"Article 107162"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trophic transfer effects of PS nanoplastics and field-derived nanoplastics in the freshwater clam Corbicula fluminea.","authors":"Oihana Latchere, Isabelle Métais, Hanane Perrein-Ettajani, Magalie Lemoing, Agnès Feurtet-Mazel, Patrice Gonzalez, Guillemine Daffe, Julien Gigault, Charlotte Catrouillet, Amélie Châtel, Magalie Baudrimont","doi":"10.1016/j.aquatox.2024.107160","DOIUrl":"https://doi.org/10.1016/j.aquatox.2024.107160","url":null,"abstract":"<p><p>Plastic pollution is of global concern. Many studies investigated the effect of micro and nanoplatics towards aquatic organisms. However, relatively few studies were assessed on freshwater organisms. Another aspect of this pollution is the impact of trophic transfer on plastic distribution and on food chain in order to evaluate its potential risk towards environmental and human health. In this context, the objective of this study was to assess the ecotoxicological impacts of different types of nanoplastics (NPs) on freshwater organisms exposed through trophic transfer. Freshwater microalgae Scenedesmus subspicatus were contaminated for 48 h with realistic concentrations of NPs (0.008, 10 and 100 µg/L). Two types of NPs were tested: commercial PS NPs and NPs generated from macro-sized plastics collected in the field (ENV NPs). Freshwater Corbicula fluminea bivalves were then fed with the contaminated algae every 48 h for 21 days. Results showed that trophic exposure led to the induction of oxidative stress (CAT activity). Overall, NPs trophic exposure caused downregulations of genes implicated in many cellular processes (immunity, oxidative stress, neurotoxicity, endocytosis, apoptosis). This present study allowed to demonstrate the relevance of investigating the trophic transfer effects of NPs on a freshwater trophic chain. Further studies should focus more on larger levels of the food chain.</p>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"107160"},"PeriodicalIF":4.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical-defensome and whole-transcriptome expression of the silverside fish Basilichthys microlepidotus in response to chronic pollution in the Maipo River basin, Central Chile","authors":"Jorge Cortés-Miranda ,&nbsp;David Veliz ,&nbsp;Noemí Rojas-Hernández ,&nbsp;Ciro Rico ,&nbsp;Catalina Gutiérrez ,&nbsp;Caren Vega-Retter","doi":"10.1016/j.aquatox.2024.107159","DOIUrl":"10.1016/j.aquatox.2024.107159","url":null,"abstract":"<div><div>Pollution is a major global concern affecting biodiversity, particularly of freshwater species. Populations have developed mechanisms to deal with pollution, such as the chemical defensome, which is a set of genes involved in maintaining internal stability. Pollution significantly affects the Maipo River basin in Chile. This area is home to the endemic silverside fish <em>Basilichthys microlepidotus</em>, whose populations are affected by pollution to different degrees. We assessed gene expression in the liver and gill of this species, focusing on whole-transcriptome and chemical-defensome levels, to identify both independent and shared mechanisms in response to pollution. The results showed that 14–18 genes were consistently expressed differently among populations in polluted areas. These genes were primarily involved in liver cell mitosis and in responses to organic chemicals and carcinogenic processes. Genes expressed differently in the gill were more abundant in immune system biological processes. All populations consistently downregulated chemical-defensome genes in the liver. In differentially expressed chemical-defensome genes, shared biological processes included virus response, cellular redox homeostasis and transport, organic cyclic compound response and DNA-templated transcription regulation. Studying chemical-defensome genes can help reveal common ways that pollution builds up over time, and examining the whole transcriptome can elucidate the context in which this response develops.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"Article 107159"},"PeriodicalIF":4.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoplastics and bisphenol A exposure alone or in combination induce hepatopancreatic damage and disturbances in carbohydrate metabolism in the Portunus trituberculatus","authors":"Xiaotian Wang ,&nbsp;Shangjie Zhou ,&nbsp;Yutong Huang ,&nbsp;Pengfei Chu ,&nbsp;Long Zhu ,&nbsp;Xiaocong Chen","doi":"10.1016/j.aquatox.2024.107145","DOIUrl":"10.1016/j.aquatox.2024.107145","url":null,"abstract":"<div><div>Bisphenol A (BPA) is a widely found endocrine-disrupting chemical (EDC). Nanoplastics (NPs) represent a novel environmental pollutant, and the combined toxicity of these pollutants on the hepatopancreas of marine arthropods is understudied. To investigate the potential risks associated with co-exposure to BPA and NPs on the hepatopancreas, <em>Portunus trituberculatus</em> was treated with 100 μg/L BPA, 10⁴ particles/L NPs, and a combination of 100 μg/L BPA + 10⁴ particles/L NPs for 21 days, respectively. Histological observation demonstrated that co-exposure severely damaged both hepatopancreas tissue and mitochondrial structure. Transcriptome analysis revealed that 1498 transcripts were differentially expressed under different exposure conditions, and these transcripts are involved in biological processes such as cellular processes and carbohydrate metabolism. BPA and NPs co-exposure modulate pyruvic acid (PA) levels by increasing the activity of pyruvate kinase (PK), leading to changes in glycogen and glucose (GLU) content within tissues, thus affecting glycolysis. The dysregulation of the <em>CHI3L1, ACSS2</em> and <em>ACYP2</em> genes induced by BPA and NPs co-exposure may collectively regulate the process of carbohydrate metabolism. Notably, the downregulation of the <em>VPS4</em> gene and the upregulation of the <em>GBA1, Pin1</em> and <em>CCND2</em> gene may affect the cell cycle, potentially impacting cell proliferation after BPA and NPs co-exposure. These data indicate that co-exposure to BPA and NPs is more significantly cytotoxic and leads to changes in carbohydrate metabolism, cell proliferation, and histological damage in the hepatopancreas of <em>P. trituberculatus</em>. This knowledge emphasizes the need for proactive measures to mitigate the adverse effects of these environmental pollutants on human and ecological health while also providing valuable insights into the relevant molecular mechanisms.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"Article 107145"},"PeriodicalIF":4.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proanthocyanidins mitigate the toxic effects in loach (Misgurnus anguillicaudatus) exposed to phenanthrene via Nrf2/NF-κB signaling pathway","authors":"Qiongya Fang ,&nbsp;Ke Li ,&nbsp;Xinyu Zhang ,&nbsp;Xiaorui Liu ,&nbsp;Siqi Jiao ,&nbsp;Li Sun ,&nbsp;Min Li ,&nbsp;Guiqin Wang ,&nbsp;Yidi Kong","doi":"10.1016/j.aquatox.2024.107158","DOIUrl":"10.1016/j.aquatox.2024.107158","url":null,"abstract":"<div><div>Phenanthrene (PHE) is a typical polycyclic aromatic hydrocarbon compound that is ubiquitous in the environment and accumulates in aquatic products, thereby posing a risk to food safety. Oligomeric proanthocyanidins (OPC) is widely distributed powerful antioxidants with potent antioxidant and anti-inflammatory properties. This study aimed to evaluate the alleviating effects of dietary OPC on oxidative stress, inflammatory suppression, and tissue damage caused by PHE exposure in loach (<em>Misgurnus anguillicaudatus</em>). In the study, loach was continuously exposed to 2.36 mg/L PHE for 28 days, after which they were fed a basal diet supplemented with 0, 200, 400, or 800 mg/kg OPC. The results displayed that PHE exposure resulted in significantly increased levels of liver health parameters (AST, ALT, COR, LDH, and ADA) compared to the control group (<em>P</em> &lt; 0.05). The PHE-exposed fish showed the lowest levels of antioxidant enzymes (CAT, SOD, GSH, GST, GSH-Px, and GR) and the greatest levels of oxidative stress parameters (ROS and MDA). PHE exposure resulted in down-regulation of <em>nrf2, ho-1, gsh-px, gst</em>, and <em>nqo-1</em>, and up-regulation of <em>keap-1</em> gene expressions in loach (<em>P</em> &lt; 0.05). Moreover, PHE-induced decreased the levels of immunity indicators (CRP, MPO, C3, C4, IgM, and LYS). An up-regulation of pro-inflammatory genes (<em>nf-κb, il-1β, il-8, il-6, il-12</em>, and <em>tnf-α</em>) and a down-regulation of anti-inflammatory gene <em>il-10</em> were the consequences of the PHE exposure. In addition, tissues showed histopathological alterations including vacuolization (liver), displaced nuclei (liver), atrophy (gills), glomerular congestion (kidney), and inflammatory cell infiltration (spleen) caused by PHE. Notably, dietary supplementation of OPC augmented immuno-antioxidant parameters, including their key genes, reduced oxidative stress and immunosuppression, and ameliorated tissue damage compared to fish exposed to PHE. In summary, supplementation with 400 mg/kg OPC in the diets could effectively alleviate the oxidative damage and inflammatory response induced by PHE exposure in loach through the Nrf2/NF-κB signaling pathway and enhance the defense ability against toxic substances of loach.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"Article 107158"},"PeriodicalIF":4.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a cell culture from the frog Leptodactylus fuscus as a model for (eco)toxicological assays","authors":"Yure Jefferson da Cruz do Nascimento ,&nbsp;Danilo Serrão Moutinho ,&nbsp;Cesar Martins ,&nbsp;Adauto Lima Cardoso ,&nbsp;Lílian Lund Amado","doi":"10.1016/j.aquatox.2024.107157","DOIUrl":"10.1016/j.aquatox.2024.107157","url":null,"abstract":"<div><div><em>Leptodactylus fuscus</em> is a frog species widely distributed in the Neotropical region, occurring in several biomes, which makes it a potential biomonitor of environmental conditions<em>.</em> To advance the establishment of this species in this field, we developed a fibroblast-like cell line derived from the digits of the forelimbs (LFUfd) and evaluated its response to possible environmental stressors. An adult male L. <em>fuscus</em> was collected, anesthetized, and euthanized with eugenol. Digits from the forelimbs were extracted, decontaminated, and dissociated. Cells were plated and cultivated in vented flasks with DMEM/F12+GlutaMAX medium and 20% FBS at 28 °C. On the 15th passage, we made the chromosome preparations, where we placed them on slides and stained them with Giemsa for karyotype analysis. The cellular response to multiple stressors (Temperature, Hydrogen Peroxide, and Aluminum) was evaluated through the MTT assay. The temperature test evaluated two groups (28 °C and 37 °C for 24 h). The effect of hydrogen peroxide (H₂O₂) was analyzed by treatment for 1 h at 28 °C in different concentrations (control, 2 μM, 20 μM, 50 μM, 100 μM and 200 μM). The effects of aluminum have been tested at three concentrations (0.5 mg/L, 1 mg/L, and 10 mg/L). The karyotype obtained showed 22 chromosomes, according to the description of the species, although a small proportion of aneuploid cells was observed (∼7%). At 37 °C there was a reduction in cell survival and no effects on cell viability at the H₂O₂ concentrations tested; however, there was a decrease in cell viability when exposed to aluminum in all the concentrations tested.. The growth behavior and karyotype observed indicate the establishment of a continuous cell culture with a stable genome that responds to environmental changes.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"Article 107157"},"PeriodicalIF":4.1,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An adverse outcome pathway approach linking retinoid signaling disruption to teratogenicity and population-level outcomes","authors":"Audrey Phan,&nbsp;Aleksandra Sokolova,&nbsp;Klara Hilscherova","doi":"10.1016/j.aquatox.2024.107143","DOIUrl":"10.1016/j.aquatox.2024.107143","url":null,"abstract":"<div><div>Recent research efforts in endocrine disruption have focused on evaluating non-EATS (estrogen, androgen, thyroid, and steroidogenesis) pathways. Retinoid signaling disruption is noteworthy because of its teratogenic effects and environmental relevance. However, current environmental risk assessments are limited in their ability to evaluate impacts on individuals and populations. This study characterizes an Adverse Outcome Pathway (AOP) network linking retinoid signaling disruption to teratogenicity and survival in zebrafish. We identified Retinoic Acid Receptor (RAR) overactivation as the molecular initiating event leading to key events including craniofacial (CFM) and tail (TM) malformations, posterior swim bladder (SB) non-inflation, impaired swimming performance, and reduced feeding, ultimately resulting in decreased survival. Our study (1) determines critical sensitivity windows for CFM, posterior SB non-inflation, and TM, (2) provides quantitative measurements for CFM and TM, and (3) defines impacts on higher biological levels including food ingestion, swimming, and survival. Results show that all-trans retinoic acid (ATRA) induces strong teratogenic effects with sensitivity windows between 4 and 48 h post fertilization (hpf) for CFM, TM, and posterior SB non-inflation. TM is the most sensitive indicator, with EC₅₀ of 0.2 - 0.26 µg/L across exposure windows 4–48, 4–72, 4–96, and 4–120 hpf. Besides inducing known malformations, ATRA impaired posterior SB inflation with EC₅₀ of 1 - 1.21 µg/L across the same exposure windows. ATRA exposure (1 µg/L) resulted in 50 % food ingestion inhibition at 7 days post fertilization (dpf) and 10 % survival at 14 dpf. This study provides a regulatory-relevant framework linking developmental effects to population outcomes, highlighting ecological risks and needs for improved risk assessments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"Article 107143"},"PeriodicalIF":4.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion-exchange chromatography in the assessment of environmental pollution with chlortetracycline","authors":"Łukasz Sikorski ,&nbsp;Agnieszka Bęś ,&nbsp;Elżbieta Karetko-Sikorska ,&nbsp;Wojciech Truszkowski ,&nbsp;Katarzyna Tomaszewska","doi":"10.1016/j.aquatox.2024.107142","DOIUrl":"10.1016/j.aquatox.2024.107142","url":null,"abstract":"<div><div>Chemical substances such as drugs pose a threat to the environment. One of the substances recorded in soil and water is chlortetracycline, an antibiotic used in veterinary medicine. Plants exposed to such xenobiotics show changes in the content of biogenic amines. An analytical technique - ion exchange chromatography is used to assess their content. The occurrence of these active compounds is used to determine the degree of environmental pollution with chemical substances. The study aimed to evaluate the toxicity of chlortetracycline (CTC) at concentrations of 0; 0.05; 0.1; 0.2 0.5;1; 2; 3; and 5 mM towards the test organism <em>Lemna minor</em>, and determine the content of biogenic amines in the plant tissues. The content of biogenic amines was analyzed by ion-exchange chromatography with post-column ninhydrin derivatization and photometric detection. The Lemna test proved that increasing concentrations of CTC had a toxic effect on the plants. It was calculated that the Lowest Observed Effects Concentration (LOEC) of CTC at &gt;0.04 mM and &gt;0.05 mM was phytotoxic to L. <em>minor</em> growth and yield. It was determined that the levels of histamine, tyramine, and cadaverine exhibited an increase, reaching 1.04, 1.90, and 3.10 µg g^-1 of tissue at 2.00 mM CTC. Simultaneously, spermine and putrescine increased to 1.21 and 3.89 µg g^-1 of tissue at concentrations of 0.10 and 0.50 mM of the drug. Conversely, the study revealed an over 88 % reduction in spermidine in plants at 5 mM of CTC. Using ion-exchange chromatography, analysis of biogenic amines, particularly spermidine and cadaverine, highlighted these intra-tissue compounds as sensitive biomarkers for water contamination with the tested drug. This research confirmed that the Lemna test is effective for assessing CTC toxicity and that ion-exchange chromatography is useful for evaluating environmental pollution by this antibiotic.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"277 ","pages":"Article 107142"},"PeriodicalIF":4.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}