Aquatic Toxicology最新文献

{"title":"Life cycle toxicity evaluation of coated magnetite nanoparticles to the amphipod Hyalella curvispina","authors":"Nuria Guadalupe Espert ,&nbsp;Jorgelina Luján Villanova ,&nbsp;Sofía Ayelén Vedelago ,&nbsp;Mariana Noelia Mardirosian ,&nbsp;Malena Morell ,&nbsp;Cecilia Inés Lascano ,&nbsp;Andrés Venturino","doi":"10.1016/j.aquatox.2025.107406","DOIUrl":"10.1016/j.aquatox.2025.107406","url":null,"abstract":"<div><div>The presence of organic pollutants in aquatic environments requires effective solutions to reduce their concentration. In this context, the development of environmental remediation technologies using nanomaterials has emerged as a promising alternative. However, prior to their application, it is necessary to evaluate the toxicity of these materials along their lifecycle. The aim of this study was to analyze the acute toxicity and behavioral alterations triggered by exposure to magnetite nanoparticles coated with oleic acid (NPOA), to anthracene (ANT) as a model contaminant to be removed, and to the different remaining products after a simulated NPOA remediation procedure of ANT-contaminated water in the native amphipod <em>Hyalella curvispina</em>. Our results show no significant mortality with ANT up to 2 mg/L; however, behavioral alterations were observed from 24-h exposure, but they diminished over time along with ANT media concentration. A trend towards increased mortality with rising concentrations of NPOA was observed up to 10 mg/L, and stabilized between 25 and 100 mg/L. Additionally, NPOA exposure caused behavioral alterations that increased with concentration and remained along the 96 hours of the bioassay. The combined assays showed no significant differences between the combined NPOA-ANT, the remaining water post-remediation and the control. In conclusion<em>,</em> neither ANT and NPOA, individually or combined, were lethal for adult <em>H. curvispina</em> individuals. Nonetheless, they impacted behavior causing stillness, altered pleopodal locomotion frequencies and decreased response to stimuli, which may affect their survival in the environment.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107406"},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic tributyltin exposure induces metabolic disruption in an invertebrate model animal, Lymnaea stagnalis","authors":"István Fodor ,&nbsp;János Schmidt ,&nbsp;Réka Svigruha ,&nbsp;Zita László ,&nbsp;László Molnár ,&nbsp;Sándor Gonda ,&nbsp;Károly Elekes ,&nbsp;Zsolt Pirger","doi":"10.1016/j.aquatox.2025.107404","DOIUrl":"10.1016/j.aquatox.2025.107404","url":null,"abstract":"<div><div>Over the last 20 years, tributyltin (TBT) has been reported to cause metabolic disruption in both invertebrates and vertebrates, highlighting the need for further detailed analysis of its physiological effects. This study aimed to investigate the metabolic-disrupting effects of TBT from the behavioral to the molecular level. Adult specimens of the great pond snail (<em>Lymnaea stagnalis</em>) were exposed to an environmentally relevant concentration (100 ng L^-1) of TBT for 21 days. After the chronic exposure, behavioral alterations as well as histological, cellular, and molecular changes were investigated in the central nervous system, kidney, and hepatopancreas. TBT exposure significantly decreased feeding activity, while locomotor activity remained unchanged. At the histological level, the cellular localization of tin was demonstrated in all tissues investigated and, in addition, characteristic morphological changes were observed in the kidney and hepatopancreas. Tissue-specific changes in lipid profiles confirmed TBT-induced disruption of lipid homeostasis in mollusks, characterized by a consistent reduction in the proportion of polyunsaturated fatty acids and a shift toward more saturated lipids. The expression of 17β-hydroxysteroid dehydrogenase type 12 (HSD17B12) enzyme, involved in lipid metabolism in vertebrates, was reduced in all three tissues after TBT exposure. Our results show that TBT induces significant multi-level metabolic changes in <em>Lymnaea</em>, including direct alterations in feeding activity and lipid composition. Our findings also suggest that HSD17B12 enzyme plays a key role in lipid metabolism in mollusks, as in mammals, and is likely involved in TBT-induced metabolic disruption. Overall, our study extends the findings of previous studies on mollusks by providing novel behavioral as well as tissue-specific histological and metabolic data and highlights the complexity and evolutionary conserved way of TBT-induced metabolic disruption.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107404"},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MCPA-Na exposure in aquatic systems: disruption of pathways and increased susceptibility to infection in fish","authors":"Yanxia Lin ,&nbsp;Ran Shi ,&nbsp;Mengzhen Wang ,&nbsp;Yali Wang ,&nbsp;Yunfan Han ,&nbsp;Yongcui Ma ,&nbsp;Liyin Li ,&nbsp;Xiaohua Xia","doi":"10.1016/j.aquatox.2025.107405","DOIUrl":"10.1016/j.aquatox.2025.107405","url":null,"abstract":"<div><div>MCPA-Na (2-methyl-4-chlorophenoxyacetic acid) is a selective herbicide widely used in agricultural cultivation. Despite monitoring indicating risks to aquatic life, the specific organ effects and pathogen susceptibility are unclear. Therefore, we constructed a “compound-core target-signaling pathway” network using network toxicology methods, and the results showed that MCPA-Na interacted with multiple organs of loach (including intestine, liver, kidney, heart, gills, skin and blood). STRING and Cytoscape software were used to screen the core targets: PPAR (Peroxisome proliferator-activated receptor), ACE (angiotensin converting enzyme), REN (Renin), and CA9 (carbonic anhydrase). KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that the core targets of each tissue were significantly enriched in the renin-angiotensin system, NF-κB signaling pathway, adherens junctions and cholinergic synapses. The relationship between the toxicology and molecular markers of MCPA-Na was further explored by using animal experiments, and the susceptibility of <em>Misgurnus anguillicaudatus</em> (loach) to opportunistic pathogens after toxic exposure was simulated by using opportunistic pathogen challenge <em>Aeromonas hydrophila</em> (<em>A. hydrophila</em>). It was found that the compound induced oxidative stress and triggered intestinal inflammation and promoted apoptosis. These processes undermine the intestinal barrier and increase the susceptibility of loach to the <em>A. hydrophila</em>, thereby exacerbating the challenge of aquaculture food safety.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107405"},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxic effects of anionic polyacrylamide on the developmental stages of Oryzias melastigma embryos and larvae","authors":"Xinya Zhao ,&nbsp;Jiangwei Zan ,&nbsp;Zhaohui Sun ,&nbsp;Xiangping Xue ,&nbsp;Hai Ren ,&nbsp;Huiru Fu ,&nbsp;Fei Si ,&nbsp;Xiaomin Jin","doi":"10.1016/j.aquatox.2025.107402","DOIUrl":"10.1016/j.aquatox.2025.107402","url":null,"abstract":"<div><div>Anionic Polyacrylamide (APAM) is widely used in oil extraction processes, serving as an oil-repellent polymer and constituting a critical component of water-based drilling fluids. The environmental and ecological effects of APAM on fishery resources have attracted significant attention, yet its toxic mechanism in marine fish at early developmental stages remains poorly understood. The potential effects of APAM on marine medaka (<em>Oryzias melastigma</em>) embryos were investigated by exposing them to 0, 120, 240, 480, and 960 mg/L for 18 d. APAM exposure caused developmental toxicity in embryos, leading to reduced heart rates, delayed and decreased hatching, increased mortality and malformations. The activities of superoxide dismutase (SOD) and catalase (CAT) initially increased after 2 d of exposure but decreased after 8 and 18 days of prolonged stress, while malondialdehyde (MDA) concentration increased, causing lipid peroxidation and worsening oxidative damage. After 18 days of APAM exposure, low and medium concentrations increased the expression of cardiovascular genes GATA4 and NKX2.5, while high concentrations decreased NKX2.5, leading to heart defects like elongated hearts and pericardial cysts. Additionally, low concentrations significantly boosted nervous system genes SHHA and SYN2A, enhancing swimming behaviors, whereas high concentrations suppressed these genes, reducing swimming activity. In conclusion, this study demonstrated that APAM exposure causes developmental toxicity, oxidative stress, neurotoxicity, and disrupts early cardiac development in <em>O. melastigma</em> embryos, providing insight into its toxic effects on early marine fish development.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107402"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatotoxic effects of exposure to different concentrations of Dibutyl phthalate (DBP) in Schizothorax prenanti: Insights from a multi-omics analysis","authors":"Luo Lei ,&nbsp;Wuga Sha ,&nbsp;Qing Liu ,&nbsp;Shidong Liu ,&nbsp;Yinhua Zhou ,&nbsp;Rundong Li ,&nbsp;Yuting Duan ,&nbsp;Suxing Fu ,&nbsp;Hejiao Li ,&nbsp;Rongrong Liao ,&nbsp;Linzhen Li ,&nbsp;Rongzhu Zhou ,&nbsp;Chaowei Zhou ,&nbsp;Haiping Liu","doi":"10.1016/j.aquatox.2025.107390","DOIUrl":"10.1016/j.aquatox.2025.107390","url":null,"abstract":"<div><div>Dibutyl phthalate (DBP) is one of the most widely used phthalate esters (PAEs) that raise increasing ecotoxicological concerns due to their harmful effects on living organisms and ecosystems. Recently, while PAEs pollution in the Yangtze River has attracted significant attention, little research has been conducted on the impact of PAEs stress on <em>S. prenanti</em>, an endemic and valuable species in the Yangtze River. In this study, one control group (C-L) and three experimental groups: T1-L (3 µg/L), T2-L (30 µg/L), and T3-L (300 µg/L) were established with reference to the DBP concentration in the environment. For the first time, we investigated the effects of DBP stress on the liver of <em>S. prenanti</em> using histomorphological, physiological, and biochemical indexes, as well as a joint multi-omics analysis. The results revealed that compared to the C-L group, liver structural damage and stress were not significant in the environmental concentration group (T1-L) and the number of differential genes and differential metabolites were lower. However, as DBP stress concentration increased, the liver damage became severe, with significant vacuolation and hemolysis observed in the T2-L and T3-L groups. The TUNEL assay revealed a significant increase in the number of apoptotic cells along with a notable rise in differential genes and metabolites in the T2-L and T3-L groups. Oxidative stress markers (T-AOC, SOD, CAT, and GSH-PX) were also significantly higher in the T2-L and T3-L groups. RNA-Seq analysis showed that the protein processing in the endoplasmic reticulum pathway was most significantly -enriched differential gene pathway shared by both C-L vs T2-L and C-L vs T3-L, with most of the genes in this pathway showing significant up-regulation. This suggests that the protein processing in the endoplasmic reticulum pathway may play a key role in protecting the liver from injuries caused by high DBP stress. Interestingly, C XI, C XII, C XIII, C XIV and C XV in the chemical carcinogenesis - reactive oxygen species pathway were significantly down-regulated in the T2-L and T3-L groups based on combined transcriptomic and metabolomic analyses, suggesting that DBP causes liver injury by disrupting mitochondria. This comprehensive histomorphometric and multi-omics study demonstrated that the current DBP concentration in the habitat of <em>S. prenanti</em> in the upper reaches of the Yangtze River temporarily causes less liver damage. However, with increasing of DBP concentration, DBP could still cause serious liver damage to <em>S. prenanti</em>. This study provides a new mechanistic understanding of the liver response mechanism of <em>S. prenanti</em> under different concentrations of DBP stress and offers basic data for the ecological protection of the Yangtze River.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107390"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanisms of neurotoxic effects from combined exposure to polystyrene and microcystin-LR in Caenorhabditis elegans","authors":"Luyu Pei ,&nbsp;Lina Sheng ,&nbsp;Yongli Ye ,&nbsp;Jia-Sheng Wang ,&nbsp;Jian Ji ,&nbsp;Xiulan Sun","doi":"10.1016/j.aquatox.2025.107403","DOIUrl":"10.1016/j.aquatox.2025.107403","url":null,"abstract":"<div><div>Microplastics (MPs) are newly emerged pollutants found in water and soil, while microcystin-leucine arginine (MC-LR) is often detected in drinking water and water products, both posing serious threats to aquatic environment and food safety. MPs can serve as carriers of MC-LR. These pollutants are often found together, rather than separately. This study focused on assessing the neurotoxicity of co-exposure to MC-LR and PS in <em>Caenorhabditis elegans</em> (<em>C. elegans</em>) after combined exposure to these two pollutants. Exposure to varying concentrations of polystyrene (PS) and MC-LR individually caused a dose-dependent decrease in the locomotion behaviors of <em>C. elegans</em>. Exposure to either of these substances alone caused damage to the phenotypic indicators of the <em>C. elegans</em>. To further explore the additional damage caused by the combined exposure of PS and MC-LR, the low, medium, and high combined dose groups were selected based on the locomotion behaviors and survival results. Combined exposure increased the level of oxidative stress indicators and resulted in neuronal loss. It also reduced serotonin, glutamate, GABA, and dopamine neurotransmitters levels, without affecting cholinergic neurons. The expression of neurotransmitter-related genes also decreased. The high-dose group showed the most significant effects. This article is the first to study the combined effect of PS and MC-LR on <em>C. elegans</em> nervous systems, offering novel insights into the risks posed by co-occurring contaminants and their implications for aquatic ecosystems and food safety.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107403"},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transgenerational effects of Nanoplastics and bisphenol A on Zebrafish lipid metabolism: Disruption of the gut Microbiota-liver axis via mTOR pathway","authors":"Zikai Liu ,&nbsp;Lanlan Li ,&nbsp;Bingbing Sun ,&nbsp;Yinhao Ding ,&nbsp;Yan lv ,&nbsp;Qing Wu ,&nbsp;Sujuan Zhao ,&nbsp;Xiang Zhang ,&nbsp;Tong Shen","doi":"10.1016/j.aquatox.2025.107401","DOIUrl":"10.1016/j.aquatox.2025.107401","url":null,"abstract":"<div><div>The gut-liver axis is vital for organism health. Nanoplastics (NPs) and bisphenol A (BPA) can harm zebrafish intestines and livers, yet their combined impact on the gut-liver axis and transgenerational effects are unknown.</div><div>In this study, F0 zebrafish were exposed to NPs and/or BPA for 28 days. Lipid indices of F0, F1, and F2 zebrafish, as well as the developmental indices of offspring, were detected. 16S rRNA sequencing and metabolomics were used to analyze F0 zebrafish gut microbiota and liver metabolites, exploring underlying mechanisms. The mTOR inhibitor Rapa was injected into F0 zebrafish to examine the mTOR pathway's role in lipid disorders caused by NPs and BPA exposure.</div><div>The results showed that the exposure of F0 generation zebrafish to NPs and BPA led to lipid metabolism disorders in all generations of zebrafish and abnormal development in F1 and F2 zebrafish. Omics analysis revealed that the combined exposure to NPs and BPA significantly exacerbated the gut microbiota disorder in F0 zebrafish. The differential metabolites identified by untargeted metabolomics were enriched in the mTOR signaling pathway. After Rapa intervention, the lipid disorders in each group of F0 zebrafish were improved.</div><div>In summary, the combined exposure to NPs and BPA may lead to lipid disorders in all generations of zebrafish and abnormal development of offspring by exacerbating the dysregulation of the gut microbiota-liver axis in F0 zebrafish. The results of this study provide mechanistic insights into the transgenerational effects induced by the combined exposure to NPs and BPA.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107401"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weak effects of chlorpyrifos at environmentally relevant concentrations on fitness-related traits in agile frogs","authors":"Zsanett Mikó ,&nbsp;Veronika Bókony ,&nbsp;Nikolett Ujhegyi ,&nbsp;Edina Nemesházi ,&nbsp;Réka Erös ,&nbsp;Stephanie Orf ,&nbsp;Attila Hettyey","doi":"10.1016/j.aquatox.2025.107400","DOIUrl":"10.1016/j.aquatox.2025.107400","url":null,"abstract":"<div><div>The widespread application of pesticides makes it important to understand the impacts of these chemicals on wildlife. Chlorpyrifos, an organophosphate insecticide that is still used <em>en masse</em> over large parts of the globe, can affect the development and behavior of non-target organisms and may thereby alter predator-prey interactions. To investigate whether environmentally relevant concentrations of chlorpyrifos affect survival, somatic, cerebral, and sexual development, as well as anti-predator behavior of the agile frog (<em>Rana dalmatina</em>), we exposed tadpoles to one of three treatments (0, 0.5, or 5 μg chlorpyrifos / L) either for three days (acute exposure) or throughout larval development (chronic exposure). We measured mortality, activity, and space use in the presence or absence of chemical cues of predatory fish, brain morphology, length of larval development, body mass at metamorphosis and two months later, and phenotypic sex. Compared to control individuals, tadpoles acutely exposed to 5 μg/L chlorpyrifos showed a shorter freezing response to predator cue on the first observation day. Also, chronic exposure to the same concentration decreased body mass at metamorphosis. Neither the chronically nor the acutely applied 0.5 μg/L chlorpyrifos concentration had any significant effect on the evaluated traits. Our results demonstrate that exposure to chlorpyrifos can induce changes in behavior and may result in lowered body mass of agile frog tadpoles, but only if the insecticide is present chronically at relatively high concentrations. Thus, agile frog tadpoles appear to be relatively tolerant to chlorpyrifos, but may suffer from its repeated high-dose application.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107400"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive review of ecological risks and toxicity mechanisms of microplastics in freshwater: Focus on zebrafish as a model organism","authors":"Haichao Sha ,&nbsp;Xi Li ,&nbsp;Qi Li ,&nbsp;Jingwei Zhang ,&nbsp;Ji Gao ,&nbsp;Lukun Ge ,&nbsp;Weinan Wang ,&nbsp;Taotao Zeng","doi":"10.1016/j.aquatox.2025.107397","DOIUrl":"10.1016/j.aquatox.2025.107397","url":null,"abstract":"<div><div>Zebrafish, as a model organism, exhibit high sensitivity to environmental pollutants and has been widely used in microplastics (MPs) toxicology studies. However, the mechanisms underlying MPs’ effects on zebrafish have yet to be comprehensively summarized. This review systematically explores the sources, pollution status of freshwater MPs and their biological toxicity mechanisms using zebrafish as a model organism. This analysis reveals that the primary sources of MPs include sediment release, natural degradation of plastic products, and precipitation-mediated transport. Freshwater MPs predominantly comprise of polyethylene (PE), polypropylene (PP), polyethylene terephthalate (PET), polystyrene (PS), and polyvinyl chloride (PVC). These MPs typically appear transparent, white, black, or blue, and predominantly exist as fibers, films, fragments, foams, and particles. The concentration, size, shape, type, aging status, and loading capacity of MPs can induce developmental malformations in zebrafish embryos, including pericardial and yolk sac edema. In adult zebrafish, MPs cause intestinal injuries characterized by increased permeability, impaired barrier function, and microbiota dysbiosis. MPs exposure also induces behavioral abnormalities such as reduced locomotion and anxiety-like responses, while simultaneously provoking oxidative stress and immune-inflammatory reactions. The physical mechanism of MPs-induced toxicity in zebrafish involves particle accumulation and tissue abrasion. In contrast, physiological and molecular mechanisms encompass interactions between MPs’ surface functional groups and biological tissues, alterations in oxidative stress markers, enzymatic activity and cytokine profiles, and modulation of gene expression patterns. This review synthesizes current knowledge on the ecological risks of freshwater MP pollution and its toxicological impacts on zebrafish, thereby providing a comprehensive framework for understanding MP toxicity mechanisms.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107397"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atrazine exposure induces abnormal swimming behavior of tadpoles under light and/or dark stimuli: A comprehensive multi-omics insights from eyes and brain","authors":"Jiawei Yin ,&nbsp;Minyi Huang ,&nbsp;Zijie Zeng ,&nbsp;Yuhao Zhang ,&nbsp;Zikang Tan ,&nbsp;Yongqiang Xia","doi":"10.1016/j.aquatox.2025.107396","DOIUrl":"10.1016/j.aquatox.2025.107396","url":null,"abstract":"<div><div>Atrazine, a widely used pesticide, can damage organs and affect the respond ability of aquatic animals to environmental stimuli. To explore the amphibian response to light and/or dark stimuli following pesticide exposure, a comparative analysis was conducted on the swimming behavior of <em>Pelophylax nigromaculatus</em> tadpoles (Gs 8 - Gs 36, from fertilised egg to forelimb appearance) following a 60-day exposure period to atrazine. Additionally, an examination of ocular structures, eye metabolism, and brain transcription was undertaken across the treatment groups. This comprehensive approach aimed to elucidate how pollutants disrupt an individual's response to light-dark stimuli by interfering with both the light-sensing organs (eyes) and the signal-processing organ (brain). Under light conditions, atrazine exposure significantly increased the total movement distance of tadpoles. In contrast, under dark conditions, atrazine induced more pronounced hyperactivity, with significant elevations in moving distance, maximum acceleration, average activity, and moving frequency. Additionally, under light/dark alternating conditions, atrazine specifically enhanced moving frequency compared to control groups. Anatomical analysis of the eyes showed that atrazine exposure led to a notable increase in the thickness of the retinal pigmented epithelium (RPE), photoreceptor layer (PL), and inner plexiform layer (IPL) in tadpoles, while significantly decreasing the thickness of the inner nuclear layer (INL), outer nuclear layer (ONL), and ganglion cell layer (GCL). Metabolic analysis of the eyes indicated significant alterations in serotonergic synapse, arachidonic acid metabolism, and linoleic acid metabolism pathways due to atrazine exposure. Additionally, transcriptomic analysis of brain tissue revealed altered neutrophil activation, granulocyte activation, and leukocyte migration pathways, accompanied by upregulated gene expression of <em>TNIP1, HAMP, CORO1A, LTA4H, RARRES2</em>, and <em>C1QA</em>. The above multi omics evidence suggests that exposure to atrazine can cause structural damage and metabolic disorders in tadpole eyes, as well as abnormal expression of photosensitive genes in the brain, ultimately leading to abnormal photoresponsive behavior in amphibians. This discovery provides a new theoretical basis for the molecular mechanism of pesticide pollutants interfering with the environmental adaptability of aquatic animals.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107396"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}