Aquatic Toxicology最新文献

{"title":"The enzymatic and transcriptional adverse effects of hull in-water cleaning discharge on juvenile rockfish (Sebastes schlegeli).","authors":"Seong Hee Mun, Kwang-Min Choi, Dongju Shin, Moonkoo Kim, Taekhyun Kim, Dae-Jung Kim, Jee-Hyun Jung","doi":"10.1016/j.aquatox.2024.107191","DOIUrl":"https://doi.org/10.1016/j.aquatox.2024.107191","url":null,"abstract":"<p><p>The hull in-water cleaning (IWC) process creates chemical contaminants, including antifouling paint particle mixtures that are directly discharged into the coastal environment. Recent attention has also been paid to the international regulation of ship hull cleaning discharges in environmental media. However, few studies have investigated the adverse effects or toxic pathways on resident marine species. In this study, we evaluated the chemical concentration of IWC discharge in situ and its toxic effects on juvenile rockfish (Sebastes schlegeli), a major coastal fishery resource, using enzymatic and transcriptomic studies. Zinc (8.05 ± 0.96 to 189.96 ± 47.76 μg/L) was the most abundant substance in IWC discharge, followed by copper (0.87 ± 0.19 to 1.97 ± 0.60 μg/L). No mortality was observed after 7 days of exposure in any experimental group; however, reactive oxygen species and acetylcholinesterase activity varied in juvenile rockfish exposed to the highest concentration of IWC discharge (10-fold-diluted IWC discharge). The immune and detoxification systems in juvenile rockfish exposed to IWC discharge were also significantly affected in juvenile rockfish exposed to 10-fold-diluted IWC discharge (zinc: 189.96 ± 47.76 μg/L, copper: 1.97 ± 0.60 μg/L). The expression of genes related to oxidative stress, including Cu/Zn-SOD, GST, and CAT, increased significantly in fish in all the exposure groups. Specifically, brain transcriptomic analysis revealed disturbances in the nervous system and homeostatic processes. Although lethal toxicity was not significantly affected, these findings indicate the potential hazard posed by sub-lethal concentrations of IWC discharge to juvenile fish, which are at a high-level in the food chain. Our enzymatic and transcriptomic results showed that Zn-dominant IWC discharge exposure may cause neuronal and immune toxicity in marine fish species, providing relevant insights into the management of hull IWC discharge to protect coastal ecosystems against chemical contaminants.</p>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"107191"},"PeriodicalIF":4.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TBBPA caused multiple intestinal injuries via ROS/NF-κB signal in common carp.","authors":"Man Qian, Yuan Geng, Jing-Jing Wang, Hong-Ru Wang, Ji-Long Luo, Xue-Jiao Gao","doi":"10.1016/j.aquatox.2024.107190","DOIUrl":"https://doi.org/10.1016/j.aquatox.2024.107190","url":null,"abstract":"<p><p>Tetrabromobisphenol A (TBBPA) is an aquatic environment's prevalent pollutant, posing a great threat to the health of aquatic animals. The intestine is a key organ for nutrient absorption as well as an important barrier to prevent pollutants from invading the body of fish. Exploring the effects of pollutants on the intestine is of great significance for maintaining fish health. Therefore, the purpose of this study was to assess the toxic effects of TBBPA on the intestine of Cyprinus carpio L. (common carp) by establishing models of common carp and primary intestinal epithelial cells exposed to TBBPA. Histological observation revealed that TBBPA exposure led to damage in the intestinal mucosa and breakage of intestinal villi. Detection of oxidative stress levels showed that TBBPA increased the levels of ROS and MDA, and decreased the activity of SOD, CAT, GSH-PX, and T-AOC in intestinal tissue and cells. Observation of inflammatory factor levels revealed that TBBPA upregulated the mRNA levels of inflammatory factors (IL-6, TNF-α, IL-1β, NF-κB p65 and IκBα). ELISA and western blotting results were consistent with the mRNA results. Moreover, TBBPA induced cell death, as evidenced by TUNEL staining and flow cytometry and confirmed by increasing levels of Bax, Cas-3, Cyt C, RIP1, RIP3, and MLKL, together with decreasing the levels of Bcl-2. TBBPA also destroyed the intestinal tight junction by reducing the mRNA and protein levels of claudin-1, ZO-1, and occludin. In summary, this study reveals that TBBPA caused intestinal injuries, inducing oxidative stress, inflammation, cell death, and tight junction disruption via ROS/NF-κB signal in common carp.</p>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"107190"},"PeriodicalIF":4.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-comprehensive approach to assess the responses of the Mediterranean mussel Mytilus galloprovincialis (Lamarck, 1919) to a simulation of a diesel-oil mixture spill","authors":"F. Bertini, V. Catania, L. Scirè Calabrisotto, M. Dara, L. Bisanti, C. La Corte, M. Staropoli, D. Piazzese, M.G. Parisi, D. Parrinello, M. Cammarata","doi":"10.1016/j.aquatox.2024.107188","DOIUrl":"https://doi.org/10.1016/j.aquatox.2024.107188","url":null,"abstract":"Oil spills are a major cause of pollution impacting marine ecosystems. In this work, the effects of short-term exposure to three different concentrations of a hydrocarbon mixture (HC), that simulated the action of such an event, were investigated on <ce:italic>Mytilus galloprovincialis</ce:italic> specimens. Physiological effects were measured using a battery of biomarkers consisting of cellular activity (phagocytosis), immune-related enzymes, chaperonins (HSP70 and HSC70), and histomorphological alterations. Different concentrations of HC led to a significant decrease in phagocytosis, especially following high concentrations. Immune-related enzymes evaluated in hemolymph and digestive gland extract showed up-regulation, suggesting the activation of antioxidant, detoxicant, and inflammatory responses. Morphological alterations of digestive gland tubules were observed after exposure to the HC. HSP70 and HSC70 activity was up regulated following the treatments, indicating their involvement in maintaining organism homeostasis. In addition, the diversity and composition of hemolymph and digestive gland microbiota exposed to HC were analyzed by automated ribosomal intergenic spacer analysis (ARISA) and a Next Generation Sequencing (NGS) approach to evaluate the connection with hydrocarbon contamination. Metagenomic analysis revealed significant differences in the hemolymph and digestive gland microbiota composition between mussels exposed and unexposed to HC. Exposure to increasing HC concentrations had a positive effect on microbial diversity with clear adaptative responses, and an increase in the relative abundance of several known degrading bacterial genera, including <ce:italic>Alcanivorax, Roseovarius, Pseudomonas, Vibrio, Oleibacter</ce:italic>. These results show the utility of a multi-comprehensive approach to evaluating functional adaptation in terms of immunological dysfunctions and microbiota alteration in the sentinel organism <ce:italic>M. galloprovincialis</ce:italic>.","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"35 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of functional groups of polystyrene nanoplastics on the neurodevelopmental toxicity of acrylamide in the early life stage of zebrafish","authors":"Haohan Yang,&nbsp;Linghui Kong,&nbsp;Zhuoyu Chen,&nbsp;Jun Wu","doi":"10.1016/j.aquatox.2024.107177","DOIUrl":"10.1016/j.aquatox.2024.107177","url":null,"abstract":"<div><div>Polystyrene nanoplastics (PS NPs) and acrylamide (ACR), both emerging contaminants, have been found to be related to neurotoxicity. However, the effects of PS NPs on ACR-induced neurodevelopmental toxicity remain unclear. In this study, anionic carboxyl polystyrene nanoplastics (PS NPs-COOH), cationic amino polystyrene nanoplastics (PS NPs-NH₂) and unmodified PS NPs were selected to investigate their interaction with ACR. A serious of the neurotoxicity biomarkers from individual to molecular level were evaluated to explore the specific mechanisms. The results indicated that the unmodified PS NPs had the most significant impact on embryonic development at low concentrations in combination with ACR. The toxicity of the other two functionalized PS NPs increased with concentration, exhibiting a clear dose-response relationship. Meanwhile, all three kinds of PS NPs significantly enhanced the impacts of ACR on the locomotion behavior of zebrafish larvae. Analysis of zebrafish nervous system development showed that PS NPs-COOH exhibit greater toxicity to the central nervous system. In contrast, PS NPs-NH₂ had a more significant impact on the motor nervous system. Gene expression analysis revealed that ACR and PS NPs significantly affected the expression levels of neurodevelopmental related genes, including <em>Neurog1, Elavl3, Gfap, Gap43, Mbpa, Shha</em>. PS NPs modified with functional groups could induce corresponding neurotoxicity by affecting genes expression related to neuronal differentiation, motor neuron, and axonal development. Based on the comprehensive biomarker response index, the order of the impacts of NPs on the neurotoxicity of ACR was PS NPs-COOH &gt; PS NPs-NH₂ &gt; PS NPs. In conclusion, this study provides new insights into the interactive biological effects of NPs and ACR on zebrafish embryo, contributing to a better understanding of their environmental risk to aquatic ecosystem.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107177"},"PeriodicalIF":4.1,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the combined impact of microplastics and emerging contaminants on fish: A review and meta-analysis","authors":"Weiming Wu ,&nbsp;Rupeng Du ,&nbsp;Zhuoyun Chen ,&nbsp;Weiwen Li ,&nbsp;Xiaomei Huang ,&nbsp;Zhong Pan","doi":"10.1016/j.aquatox.2024.107176","DOIUrl":"10.1016/j.aquatox.2024.107176","url":null,"abstract":"<div><div>Microplastics (MPs) possess unique adsorptive properties that render their surfaces prone to absorbing other contaminants. When interacting with these emerging contaminants, MPs may have unpredictable negative impacts on fish. Prior studies have primarily concentrated on the impact of single contaminants, while investigations into combined pollution have not received adequate attention. Therefore, research on combined pollution holds greater practical significance. The physiological indicators of fish affected by emerging contaminants and the mechanisms behind these effects are not yet fully clear. To address this issue, a meta-analysis was performed to evaluate the impact of combined pollution of MPs-containing emerging contaminants on various aspects of fish health, encompassing behavior, consumption, development, and reproduction, along with the assessment of oxidative stress and neurotoxicity of fish. The results of the meta-analysis indicated that combined pollution adversely impacted fish reproduction, development, oxidative stress, and neurotoxicity. Importantly, significant differences were observed between fish species regarding their susceptibility to function and oxidative stress. Further investigation into the mechanisms of the impact of combined pollution on fish revealed that the magnitude of this impact is closely associated with the characteristics of the MPs themselves. MPs with higher adsorption capacities tend to lead to more severe consequences, while the impact of MPs with lower adsorption capacities relies more on their toxicity. Nevertheless, a close correlation between the duration of exposure to combined pollution and the level of oxidative stress in fish was not identified. Through a systematic analysis of existing studies, this review not only explored the cumulative effects of combined pollution on fish but also highlighted the intricate nature of such pollution within aquatic ecosystems. It contributes to the growing body of knowledge on the subject and emphasizes the need for further research to unravel the complexities associated with the combined impact of MPs-containing emerging contaminants on aquatic life.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107176"},"PeriodicalIF":4.1,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glyphosate and glyphosate-based herbicides induce Poecilia reticulata to maintain redox equilibrium during and after coexposure to iron oxide nanoparticles (y-Fe2O3)","authors":"João Marcos de Lima Faria ,&nbsp;Mariana Morozesk ,&nbsp;Iara da Costa Souza ,&nbsp;Victória Costa da Silva ,&nbsp;Luiz Arthur Mendes Bataus ,&nbsp;Simone Maria Teixeira de Sabóia-Morais ,&nbsp;Marisa Narciso Fernandes","doi":"10.1016/j.aquatox.2024.107175","DOIUrl":"10.1016/j.aquatox.2024.107175","url":null,"abstract":"<div><div>Iron oxide nanoparticles (IONPs) are being increasingly recognized as viable materials for environmental remediation due to their capacity to adsorb contaminants such as glyphosate (GLY) on their surfaces. Nevertheless, the ecotoxicological implications of IONPs associated with GLY necessitate thorough evaluation to ascertain the safety of such remediation strategies. In this context, the present investigation was conducted to examine hepatic biomarkers pertinent to the redox system, as well as ultrastructural hepatic alterations in <em>Poecilia reticulata</em>, following a 21-day exposure to environmentally relevant concentrations of IONPs, iron ions (Fe), and glyphosate in its pure form (GLY) as well as a commercial glyphosate-based herbicide (GBH). After this exposure, the fish underwent a 21-day recovery in uncontaminated water. The results indicated an increase in the activity of catalase (CAT) and glutathione S-transferase (GST) and in the concentration of glutathione (GSH) in the animals subjected to IONP+GBH and IONP+GLY treatments. This biochemical response persisted for the duration of both the exposure and recovery phases. Concurrently, hepatocytes displayed mitochondria with increased electron density, augmented lipid droplet accumulation, and expanded necrotic areas within the hepatic tissue. In contrast, fish exposed solely to IONPs exhibited sustained redox homeostasis throughout the investigative timeline. These findings suggest that the coexposure toxicity of IONP+GLY and IONP+GBH is attributable to the agent adsorbed onto the IONPs and that <em>P. reticulata</em> could maintain an active antioxidant defense mechanism throughout the entire study period.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107175"},"PeriodicalIF":4.1,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal Daphnia magna exposure to the antidepressant sertraline causes molting disorder, multi-generational reproductive and serotonergic dysfunction","authors":"Chenglong Dong ,&nbsp;Liqiang Wang ,&nbsp;Nikolai Barulin ,&nbsp;Juan José Alava ,&nbsp;Shaoquan Liu ,&nbsp;Dongmei Xiong","doi":"10.1016/j.aquatox.2024.107161","DOIUrl":"10.1016/j.aquatox.2024.107161","url":null,"abstract":"<div><div>Sertraline, one of the most commonly used antidepressants, has exhibited a progressively escalating trend in usage over the course of the last decades years, which have been exacerbated by the COVID-19 pandemic. Here, this study assessed the transgenerational effects of sertraline on the aquatic microcrustacean <em>Daphnia magna</em>, a parthenogenetic model species. The parental <em>D. magna</em> (G0) were exposed to environmentally relevant concentrations of sertraline (0.1 and 10 μg/L) for 21 days at individual and population level, and observed exposure triggered specific increased fecundity and desynchronized molting. These alterations were partially inherited through three subsequent non-exposed generations (G1, G2, and G3), as evidenced by increased fecundity and disordered molting in G1, reduced fecundity in G2, and reduced body size of G3-offspring. The molt-related genes <em>neverland 1</em> and <em>hormone receptor 3</em> were significantly different to the control group simultaneously only in the exposed generation, which may well be responsible for the molting asynchrony. Vitellogenin plays an important role in reproduction, and our results indicate that its abnormal expression persists up to G3, which was highly correlated with the expression of <em>serotonin transporter</em>, the drug target of sertraline. This finding suggested that sertraline possesses a sustained reproductive toxicity and disrupting potential and may be associated with serotonin dysregulation caused by compensatory feedback of <em>serotonin transporter</em>. In combination with male birth and upregulation of <em>doublesex</em> and <em>vitellogenin</em>, sertraline was deemed to trigger a self-defense response of <em>D. magna</em>, known as “abandon-ship” by increasing reproductive inputs. However, no males was found in individual reproduction test in each generation, which may suggest some interaction between sertraline and population density. Our findings emphasize that the toxic effects of sertraline can be transferred to unexposed generations, even with different adverse consequences, implying that future studies need to focus on transgenerational delayed effects and the underlying mechanisms.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107161"},"PeriodicalIF":4.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics and potential human health risks of Paralytic Shellfish Toxins identified in eight species of bivalves from South Yellow Sea Mudflat","authors":"Cheng Ding ,&nbsp;Jinling Wu ,&nbsp;Qingyuan Guo ,&nbsp;Jiaxuan Luan ,&nbsp;Kai Yang ,&nbsp;Zhaoxia Li ,&nbsp;Xuan Li ,&nbsp;Jianwei Yu ,&nbsp;Feng Liang ,&nbsp;Bairen Yang ,&nbsp;Tianming Chen","doi":"10.1016/j.aquatox.2024.107174","DOIUrl":"10.1016/j.aquatox.2024.107174","url":null,"abstract":"<div><div>The consumption of bivalves contaminated with paralytic shellfish toxins (PSTs) poses a serious risk to human health. However, the presence of PSTs in bivalves from the South Yellow Sea Mudflat remains unclear. This study comprehensively examined the characteristics and potential health risks of PSTs in eight species of bivalves from the South Yellow Sea Mudflat across four seasons. Typical PSTs, including STX, dcNeoSTX, GTX1, GTX2, GTX3, and GTX4, were detected in white clams, clams, short-necked clams, blue mussels, razor clams, mussels, scallops, and oysters. Significant differences of PSTs concentrations among bivalves across different seasons were detected using Kruskal-Wallis tests (<em>p</em> &lt; 0.05), with the highest PSTs concentrations found in mussels (20.46 μg/individual) during autumn. Furthermore, Pearson tests revealed significant positive correlations between PSTs concentrations and shell length, shell height, shell width, and soft tissue wet weight, indicating that larger bivalves contain higher PSTs levels. The highest dietary toxin intake (DTI) of PSTs across the four seasons was found in mussels (2.138 μgSTX eq. kg⁻¹ bw day⁻¹) during autumn. Notably, the exposure risk index (ERI) from bivalve consumption for male consumers was 1.23 ± 0.819, which was higher than that for female consumers (1.102 ± 0.735). The ERI of PSTs for children aged 2–7 and the elderly over 65 were 1.448 ± 0.957 and 1.316 ± 0.874, respectively, which were higher than those for other age groups, indicating that children and the elderly are more sensitive to PSTs. It is important to note that most ERIs of PSTs from total tissues were higher than 1 (potential risk), while ERIs of PSTs from non-digestive tissues were lower than 1, suggesting that potential health risks could be reduced by removing the digestive tissues of bivalves before consumption. This study provides valuable information for mitigating health risks associated with bivalve consumption.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107174"},"PeriodicalIF":4.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a cell culture from Daphnia magna as an in vitro model for (eco)toxicology assays: Case study using Bisphenol A as a representative cytotoxic and endocrine disrupting chemical","authors":"Sreevidya CP ,&nbsp;Manoj Kumar TM ,&nbsp;Soumya Balakrishnan ,&nbsp;Suresh Kunjiraman ,&nbsp;Manomi Sarasan ,&nbsp;Jason T. Magnuson ,&nbsp;Jayesh Puthumana","doi":"10.1016/j.aquatox.2024.107173","DOIUrl":"10.1016/j.aquatox.2024.107173","url":null,"abstract":"<div><div>Bisphenol A (BPA) is a widely used industrial compound found in polycarbonate plastics, epoxy resin, and various polymer materials, leading to its ubiquitous presence in the environment. The toxicity of BPA to aquatic organisms has been well documented following <em>in vivo</em> exposure scenarios, with known cytotoxic and endocrine-disrupting effects. As such, BPA was used in this study as a well-characterized chemical to implement more ethical and resource-efficient scientific practices in toxicity testing through new approach methods (NAMs). Due to the frequent use of <em>Daphnia</em> spp. as a model organism in toxicology, we developed an <em>in vitro</em> cell culture system from <em>Daphnia magna</em> embryos, with optimized medium to support cell longevity. The cultures were maintained for up to two months, demonstrating their stability and suitability for cytotoxicity studies. Using this novel system, lethal concentration 50 (LC₅₀) values were determined at the 24 and 48 h time points following BPA exposure. Subsequently, oxidative stress, endocrine disruption, and DNA damage were assessed through gene expression, activity assays, and a comet assay in BPA-exposed cells. LC₅₀ values of 52 µM and 20 µM BPA were calculated after 24 and 48 h exposures, respectively. BPA cells exposed to 20 and 52 µM had significantly increased GSH, GPx, and GST activity levels. mRNA expression analysis revealed significant upregulations in the expression of <em>hsp70, hsp90, gst, gpx, vtg1</em>, and <em>cyp4</em>, with downregulations of <em>sod, cat</em>, and <em>ecr</em> following BPA exposure. Furthermore, comet assays showed a significantly higher level of DNA damage induced by BPA compared to controls, with greater comet and tail lengths. This study established a novel <em>in vitro Daphnia</em> model, using BPA as a case study for determining toxic effects, further highlighting the importance and applicability of utilizing alternative methods in ecotoxicological research through reducing animal use.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107173"},"PeriodicalIF":4.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of physiology, microbiota and metabolomics reveals toxic response of zebrafish gut to co-exposure to polystyrene nanoplastics and arsenic","authors":"Guoxin Li ,&nbsp;Min Lv ,&nbsp;Haitao Yu ,&nbsp;Heng zhang ,&nbsp;Dandan Zhang ,&nbsp;Qingsong Li ,&nbsp;Lei Wang ,&nbsp;Yicheng Wu","doi":"10.1016/j.aquatox.2024.107172","DOIUrl":"10.1016/j.aquatox.2024.107172","url":null,"abstract":"<div><div>Both nanoplastic (NP) particles and arsenic (As) are widespread in aquatic environments and pose a combined risk of exposure to aquatic organisms. How the gut of aquatic organisms responds to combined risk of exposure is still unclear. In this study, zebrafish (<em>Danio rerio</em>) were subjected to three distinct As stress environments: only As group (10 μg/L), and As combined with different concentrations of polystyrene (PS) NPs (1 mg/L and 10 mg/L) groups for 21 days via semi-static waterborne exposure. The physiological responses to combined stress, the diversity of gut microorganisms, and the metabolomic response of the gut were investigated. The findings indicated that PSNPs were prevalent in the intestines of zebrafish in the co-exposed group. Furthermore, the administration of 1 mg/L and 10 mg/L of PSNPs in the co-exposed group was observed to elevate As levels in the intestines by 24.88% and 76.95%, respectively, in comparison to As treatment alone. Simultaneous exposure of the gut to PSNPs and As resulted in increased contents/activities of MDA, SOD, CAT, and GST, and a decrease in contents/activities of GSH and GPx, when compared to As exposure alone. Additionally, the combined exposure led to an elevated expression of the <em>Cu/Zn-sod, Mn-sod, gpx</em>, and <em>cat</em> genes. The combined treatment with NPs and As resulted in an increase in the abundance of Proteobacteria and Fusobacteriota at the phylum level, as well as a significant increase in the abundance of <em>Cetobacterium, Rhodococcus</em>, and <em>Bacteroides</em> at the genus level. Non-targeted metabolomics analyses suggest that metabolic pathways affected by co-exposure include glycerophospholipid metabolism, glycerolipid metabolism, ABC transporters and autophagy. The findings of this study are of considerable significance for the evaluation of the toxicological impact of co-existing pollutants.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"278 ","pages":"Article 107172"},"PeriodicalIF":4.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}