Aquatic Toxicology最新文献

{"title":"Toxic effects of anionic polyacrylamide on the developmental stages of Oryzias melastigma embryos and larvae","authors":"Xinya Zhao ,&nbsp;Jiangwei Zan ,&nbsp;Zhaohui Sun ,&nbsp;Xiangping Xue ,&nbsp;Hai Ren ,&nbsp;Huiru Fu ,&nbsp;Fei Si ,&nbsp;Xiaomin Jin","doi":"10.1016/j.aquatox.2025.107402","DOIUrl":"10.1016/j.aquatox.2025.107402","url":null,"abstract":"<div><div>Anionic Polyacrylamide (APAM) is widely used in oil extraction processes, serving as an oil-repellent polymer and constituting a critical component of water-based drilling fluids. The environmental and ecological effects of APAM on fishery resources have attracted significant attention, yet its toxic mechanism in marine fish at early developmental stages remains poorly understood. The potential effects of APAM on marine medaka (<em>Oryzias melastigma</em>) embryos were investigated by exposing them to 0, 120, 240, 480, and 960 mg/L for 18 d. APAM exposure caused developmental toxicity in embryos, leading to reduced heart rates, delayed and decreased hatching, increased mortality and malformations. The activities of superoxide dismutase (SOD) and catalase (CAT) initially increased after 2 d of exposure but decreased after 8 and 18 days of prolonged stress, while malondialdehyde (MDA) concentration increased, causing lipid peroxidation and worsening oxidative damage. After 18 days of APAM exposure, low and medium concentrations increased the expression of cardiovascular genes GATA4 and NKX2.5, while high concentrations decreased NKX2.5, leading to heart defects like elongated hearts and pericardial cysts. Additionally, low concentrations significantly boosted nervous system genes SHHA and SYN2A, enhancing swimming behaviors, whereas high concentrations suppressed these genes, reducing swimming activity. In conclusion, this study demonstrated that APAM exposure causes developmental toxicity, oxidative stress, neurotoxicity, and disrupts early cardiac development in <em>O. melastigma</em> embryos, providing insight into its toxic effects on early marine fish development.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107402"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatotoxic effects of exposure to different concentrations of Dibutyl phthalate (DBP) in Schizothorax prenanti: Insights from a multi-omics analysis","authors":"Luo Lei ,&nbsp;Wuga Sha ,&nbsp;Qing Liu ,&nbsp;Shidong Liu ,&nbsp;Yinhua Zhou ,&nbsp;Rundong Li ,&nbsp;Yuting Duan ,&nbsp;Suxing Fu ,&nbsp;Hejiao Li ,&nbsp;Rongrong Liao ,&nbsp;Linzhen Li ,&nbsp;Rongzhu Zhou ,&nbsp;Chaowei Zhou ,&nbsp;Haiping Liu","doi":"10.1016/j.aquatox.2025.107390","DOIUrl":"10.1016/j.aquatox.2025.107390","url":null,"abstract":"<div><div>Dibutyl phthalate (DBP) is one of the most widely used phthalate esters (PAEs) that raise increasing ecotoxicological concerns due to their harmful effects on living organisms and ecosystems. Recently, while PAEs pollution in the Yangtze River has attracted significant attention, little research has been conducted on the impact of PAEs stress on <em>S. prenanti</em>, an endemic and valuable species in the Yangtze River. In this study, one control group (C-L) and three experimental groups: T1-L (3 µg/L), T2-L (30 µg/L), and T3-L (300 µg/L) were established with reference to the DBP concentration in the environment. For the first time, we investigated the effects of DBP stress on the liver of <em>S. prenanti</em> using histomorphological, physiological, and biochemical indexes, as well as a joint multi-omics analysis. The results revealed that compared to the C-L group, liver structural damage and stress were not significant in the environmental concentration group (T1-L) and the number of differential genes and differential metabolites were lower. However, as DBP stress concentration increased, the liver damage became severe, with significant vacuolation and hemolysis observed in the T2-L and T3-L groups. The TUNEL assay revealed a significant increase in the number of apoptotic cells along with a notable rise in differential genes and metabolites in the T2-L and T3-L groups. Oxidative stress markers (T-AOC, SOD, CAT, and GSH-PX) were also significantly higher in the T2-L and T3-L groups. RNA-Seq analysis showed that the protein processing in the endoplasmic reticulum pathway was most significantly -enriched differential gene pathway shared by both C-L vs T2-L and C-L vs T3-L, with most of the genes in this pathway showing significant up-regulation. This suggests that the protein processing in the endoplasmic reticulum pathway may play a key role in protecting the liver from injuries caused by high DBP stress. Interestingly, C XI, C XII, C XIII, C XIV and C XV in the chemical carcinogenesis - reactive oxygen species pathway were significantly down-regulated in the T2-L and T3-L groups based on combined transcriptomic and metabolomic analyses, suggesting that DBP causes liver injury by disrupting mitochondria. This comprehensive histomorphometric and multi-omics study demonstrated that the current DBP concentration in the habitat of <em>S. prenanti</em> in the upper reaches of the Yangtze River temporarily causes less liver damage. However, with increasing of DBP concentration, DBP could still cause serious liver damage to <em>S. prenanti</em>. This study provides a new mechanistic understanding of the liver response mechanism of <em>S. prenanti</em> under different concentrations of DBP stress and offers basic data for the ecological protection of the Yangtze River.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107390"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanisms of neurotoxic effects from combined exposure to polystyrene and microcystin-LR in Caenorhabditis elegans","authors":"Luyu Pei ,&nbsp;Lina Sheng ,&nbsp;Yongli Ye ,&nbsp;Jia-Sheng Wang ,&nbsp;Jian Ji ,&nbsp;Xiulan Sun","doi":"10.1016/j.aquatox.2025.107403","DOIUrl":"10.1016/j.aquatox.2025.107403","url":null,"abstract":"<div><div>Microplastics (MPs) are newly emerged pollutants found in water and soil, while microcystin-leucine arginine (MC-LR) is often detected in drinking water and water products, both posing serious threats to aquatic environment and food safety. MPs can serve as carriers of MC-LR. These pollutants are often found together, rather than separately. This study focused on assessing the neurotoxicity of co-exposure to MC-LR and PS in <em>Caenorhabditis elegans</em> (<em>C. elegans</em>) after combined exposure to these two pollutants. Exposure to varying concentrations of polystyrene (PS) and MC-LR individually caused a dose-dependent decrease in the locomotion behaviors of <em>C. elegans</em>. Exposure to either of these substances alone caused damage to the phenotypic indicators of the <em>C. elegans</em>. To further explore the additional damage caused by the combined exposure of PS and MC-LR, the low, medium, and high combined dose groups were selected based on the locomotion behaviors and survival results. Combined exposure increased the level of oxidative stress indicators and resulted in neuronal loss. It also reduced serotonin, glutamate, GABA, and dopamine neurotransmitters levels, without affecting cholinergic neurons. The expression of neurotransmitter-related genes also decreased. The high-dose group showed the most significant effects. This article is the first to study the combined effect of PS and MC-LR on <em>C. elegans</em> nervous systems, offering novel insights into the risks posed by co-occurring contaminants and their implications for aquatic ecosystems and food safety.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107403"},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transgenerational effects of Nanoplastics and bisphenol A on Zebrafish lipid metabolism: Disruption of the gut Microbiota-liver axis via mTOR pathway","authors":"Zikai Liu ,&nbsp;Lanlan Li ,&nbsp;Bingbing Sun ,&nbsp;Yinhao Ding ,&nbsp;Yan lv ,&nbsp;Qing Wu ,&nbsp;Sujuan Zhao ,&nbsp;Xiang Zhang ,&nbsp;Tong Shen","doi":"10.1016/j.aquatox.2025.107401","DOIUrl":"10.1016/j.aquatox.2025.107401","url":null,"abstract":"<div><div>The gut-liver axis is vital for organism health. Nanoplastics (NPs) and bisphenol A (BPA) can harm zebrafish intestines and livers, yet their combined impact on the gut-liver axis and transgenerational effects are unknown.</div><div>In this study, F0 zebrafish were exposed to NPs and/or BPA for 28 days. Lipid indices of F0, F1, and F2 zebrafish, as well as the developmental indices of offspring, were detected. 16S rRNA sequencing and metabolomics were used to analyze F0 zebrafish gut microbiota and liver metabolites, exploring underlying mechanisms. The mTOR inhibitor Rapa was injected into F0 zebrafish to examine the mTOR pathway's role in lipid disorders caused by NPs and BPA exposure.</div><div>The results showed that the exposure of F0 generation zebrafish to NPs and BPA led to lipid metabolism disorders in all generations of zebrafish and abnormal development in F1 and F2 zebrafish. Omics analysis revealed that the combined exposure to NPs and BPA significantly exacerbated the gut microbiota disorder in F0 zebrafish. The differential metabolites identified by untargeted metabolomics were enriched in the mTOR signaling pathway. After Rapa intervention, the lipid disorders in each group of F0 zebrafish were improved.</div><div>In summary, the combined exposure to NPs and BPA may lead to lipid disorders in all generations of zebrafish and abnormal development of offspring by exacerbating the dysregulation of the gut microbiota-liver axis in F0 zebrafish. The results of this study provide mechanistic insights into the transgenerational effects induced by the combined exposure to NPs and BPA.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107401"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weak effects of chlorpyrifos at environmentally relevant concentrations on fitness-related traits in agile frogs","authors":"Zsanett Mikó ,&nbsp;Veronika Bókony ,&nbsp;Nikolett Ujhegyi ,&nbsp;Edina Nemesházi ,&nbsp;Réka Erös ,&nbsp;Stephanie Orf ,&nbsp;Attila Hettyey","doi":"10.1016/j.aquatox.2025.107400","DOIUrl":"10.1016/j.aquatox.2025.107400","url":null,"abstract":"<div><div>The widespread application of pesticides makes it important to understand the impacts of these chemicals on wildlife. Chlorpyrifos, an organophosphate insecticide that is still used <em>en masse</em> over large parts of the globe, can affect the development and behavior of non-target organisms and may thereby alter predator-prey interactions. To investigate whether environmentally relevant concentrations of chlorpyrifos affect survival, somatic, cerebral, and sexual development, as well as anti-predator behavior of the agile frog (<em>Rana dalmatina</em>), we exposed tadpoles to one of three treatments (0, 0.5, or 5 μg chlorpyrifos / L) either for three days (acute exposure) or throughout larval development (chronic exposure). We measured mortality, activity, and space use in the presence or absence of chemical cues of predatory fish, brain morphology, length of larval development, body mass at metamorphosis and two months later, and phenotypic sex. Compared to control individuals, tadpoles acutely exposed to 5 μg/L chlorpyrifos showed a shorter freezing response to predator cue on the first observation day. Also, chronic exposure to the same concentration decreased body mass at metamorphosis. Neither the chronically nor the acutely applied 0.5 μg/L chlorpyrifos concentration had any significant effect on the evaluated traits. Our results demonstrate that exposure to chlorpyrifos can induce changes in behavior and may result in lowered body mass of agile frog tadpoles, but only if the insecticide is present chronically at relatively high concentrations. Thus, agile frog tadpoles appear to be relatively tolerant to chlorpyrifos, but may suffer from its repeated high-dose application.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107400"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive review of ecological risks and toxicity mechanisms of microplastics in freshwater: Focus on zebrafish as a model organism","authors":"Haichao Sha ,&nbsp;Xi Li ,&nbsp;Qi Li ,&nbsp;Jingwei Zhang ,&nbsp;Ji Gao ,&nbsp;Lukun Ge ,&nbsp;Weinan Wang ,&nbsp;Taotao Zeng","doi":"10.1016/j.aquatox.2025.107397","DOIUrl":"10.1016/j.aquatox.2025.107397","url":null,"abstract":"<div><div>Zebrafish, as a model organism, exhibit high sensitivity to environmental pollutants and has been widely used in microplastics (MPs) toxicology studies. However, the mechanisms underlying MPs’ effects on zebrafish have yet to be comprehensively summarized. This review systematically explores the sources, pollution status of freshwater MPs and their biological toxicity mechanisms using zebrafish as a model organism. This analysis reveals that the primary sources of MPs include sediment release, natural degradation of plastic products, and precipitation-mediated transport. Freshwater MPs predominantly comprise of polyethylene (PE), polypropylene (PP), polyethylene terephthalate (PET), polystyrene (PS), and polyvinyl chloride (PVC). These MPs typically appear transparent, white, black, or blue, and predominantly exist as fibers, films, fragments, foams, and particles. The concentration, size, shape, type, aging status, and loading capacity of MPs can induce developmental malformations in zebrafish embryos, including pericardial and yolk sac edema. In adult zebrafish, MPs cause intestinal injuries characterized by increased permeability, impaired barrier function, and microbiota dysbiosis. MPs exposure also induces behavioral abnormalities such as reduced locomotion and anxiety-like responses, while simultaneously provoking oxidative stress and immune-inflammatory reactions. The physical mechanism of MPs-induced toxicity in zebrafish involves particle accumulation and tissue abrasion. In contrast, physiological and molecular mechanisms encompass interactions between MPs’ surface functional groups and biological tissues, alterations in oxidative stress markers, enzymatic activity and cytokine profiles, and modulation of gene expression patterns. This review synthesizes current knowledge on the ecological risks of freshwater MP pollution and its toxicological impacts on zebrafish, thereby providing a comprehensive framework for understanding MP toxicity mechanisms.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107397"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atrazine exposure induces abnormal swimming behavior of tadpoles under light and/or dark stimuli: A comprehensive multi-omics insights from eyes and brain","authors":"Jiawei Yin ,&nbsp;Minyi Huang ,&nbsp;Zijie Zeng ,&nbsp;Yuhao Zhang ,&nbsp;Zikang Tan ,&nbsp;Yongqiang Xia","doi":"10.1016/j.aquatox.2025.107396","DOIUrl":"10.1016/j.aquatox.2025.107396","url":null,"abstract":"<div><div>Atrazine, a widely used pesticide, can damage organs and affect the respond ability of aquatic animals to environmental stimuli. To explore the amphibian response to light and/or dark stimuli following pesticide exposure, a comparative analysis was conducted on the swimming behavior of <em>Pelophylax nigromaculatus</em> tadpoles (Gs 8 - Gs 36, from fertilised egg to forelimb appearance) following a 60-day exposure period to atrazine. Additionally, an examination of ocular structures, eye metabolism, and brain transcription was undertaken across the treatment groups. This comprehensive approach aimed to elucidate how pollutants disrupt an individual's response to light-dark stimuli by interfering with both the light-sensing organs (eyes) and the signal-processing organ (brain). Under light conditions, atrazine exposure significantly increased the total movement distance of tadpoles. In contrast, under dark conditions, atrazine induced more pronounced hyperactivity, with significant elevations in moving distance, maximum acceleration, average activity, and moving frequency. Additionally, under light/dark alternating conditions, atrazine specifically enhanced moving frequency compared to control groups. Anatomical analysis of the eyes showed that atrazine exposure led to a notable increase in the thickness of the retinal pigmented epithelium (RPE), photoreceptor layer (PL), and inner plexiform layer (IPL) in tadpoles, while significantly decreasing the thickness of the inner nuclear layer (INL), outer nuclear layer (ONL), and ganglion cell layer (GCL). Metabolic analysis of the eyes indicated significant alterations in serotonergic synapse, arachidonic acid metabolism, and linoleic acid metabolism pathways due to atrazine exposure. Additionally, transcriptomic analysis of brain tissue revealed altered neutrophil activation, granulocyte activation, and leukocyte migration pathways, accompanied by upregulated gene expression of <em>TNIP1, HAMP, CORO1A, LTA4H, RARRES2</em>, and <em>C1QA</em>. The above multi omics evidence suggests that exposure to atrazine can cause structural damage and metabolic disorders in tadpole eyes, as well as abnormal expression of photosensitive genes in the brain, ultimately leading to abnormal photoresponsive behavior in amphibians. This discovery provides a new theoretical basis for the molecular mechanism of pesticide pollutants interfering with the environmental adaptability of aquatic animals.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107396"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pregnane X receptor attenuates gold nanoparticles’ toxicity through accelerating zebrafish embryo hatching","authors":"Jia Hu ,&nbsp;Jingjing Tian ,&nbsp;Pai Liu ,&nbsp;Qian Zhang ,&nbsp;Ningyao Li ,&nbsp;Jian Yin","doi":"10.1016/j.aquatox.2025.107399","DOIUrl":"10.1016/j.aquatox.2025.107399","url":null,"abstract":"<div><div>It is well known that fish embryos are vulnerable to waterborne nanoparticles (NPs), with delayed hatching being the most common and sensitive endpoint. Up-regulation of hatching enzymes has been believed to be an important detoxification mechanism for NPs, but the inner mechanism for such phenomena has been seldom investigated. This study aimed to investigate the role of pregnane X receptor (Pxr) in maintaining the robustness of embryo hatching after treatment with gold nanoparticles (AuNPs, 4 and 82 nm). For this purpose, embryos from mating of 6-month-old wild-type (WT) AB strain zebrafish (<em>Danio rerio</em>, 3∼4-cm-length) were treated with AuNPs since 4 h post-fertilization (hpf). It was found that both AuNPs significantly inhibited embryo hatching after 52-h treatment, with Au-4 being more toxic at the same mass concentrations. At non-toxic concentrations and median effective concentrations (EC₅₀) of delayed hatching, both AuNPs induced the mRNA expression of HEs and Pxr at 48 hpf, and Au-4 seemed to be more effective. The induction extents of HEs by AuNPs decreased when Pxr was knocked out or inhibited, indicating the role of Pxr in such process. Additionally, knockout/inhibition of Pxr significantly delayed the hatching of embryos at 56 hpf, and activation of Pxr accelerated the process at moderate concentrations. Such phenomena correlated well with the alterations in the mRNA expression and activities of HEs, indicating a fact that AuNPs activated Pxr and up-regulated HEs, which helped the detoxification of AuNPs. RNA-sequencing analysis of WT and <em>pxr</em>-deficient embryos at 24 hpf confirmed the alteration of <em>he1.1&amp;1.2</em>. In addition, Pxr influenced mRNA encoding muscle development (muscle system process and striated muscle tissue development) and energy metabolism (carbohydrate metabolic process and ATP metabolic process), which were related to the motility of embryos and determined the hatching speed. Such function was confirmed by the reduced locomotor activity of pxr-deficient larvae at 120 hpf. Overall, these results suggested a novel role of Pxr in promoting the hatching of zebrafish embryos, which contributed to the detoxification of AuNPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107399"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of PFDA toxicity on RTgill-W1 cell line via metabolomics and lipidomics approaches","authors":"Thao V. Nguyen ,&nbsp;Anu Kumar ,&nbsp;Maryam Taraji ,&nbsp;Natoiya D.R. Lloyd","doi":"10.1016/j.aquatox.2025.107395","DOIUrl":"10.1016/j.aquatox.2025.107395","url":null,"abstract":"<div><div>Perfluorodecanoic acid (PFDA), a long-chain perfluoroalkyl substance (PFAS), is known for its environmental persistence and potential toxicity. This study evaluated PFDA toxicity in the RTgill-W1 cell line, a model for aquatic toxicology, using a combination of cell viability assays, reactive oxygen species (ROS) measurements, and high-throughput metabolomics and lipidomics. PFDA exposure resulted in significant, dose-dependent reductions in cell viability and increased ROS production, with an EC₅₀ value of 51.9 ± 1.7 mg/L, highlighting its cytotoxic potential. Metabolomic profiling revealed dose-dependent disruptions in 168 metabolites, impacting pathways related to <em>amino acid metabolism, carbohydrate metabolism, lipid metabolism, vitamin and cofactor metabolism</em>, and <em>nucleotide metabolism</em>. Furthermore, lipidomic analysis identified 102 significantly altered lipids, primary affecting <em>glycerolipid metabolism, fatty acid biosynthesis, glycerophospholipid metabolism, sphingolipid metabolism -</em> suggesting compromised membrane integrity, energy production, and signalling processes. These findings underscore PFDA’s capacity to interfere with critical cellular processes and highlight the utility of integrated omics approaches in elucidating the molecular mechanisms of PFAS toxicity. Future studies should focus on validating fish cell assays through short-term <em>in vivo</em> tests to enhance their reliability and ecological relevance.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107395"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicological impact of the fungicide tebuconazole on fish: a historical review, global trends and challenges","authors":"Bianca Leite Carnib ,&nbsp;Felipe Cirqueira ,&nbsp;Jerusa Maria de Oliveira ,&nbsp;Thiago Lopes Rocha","doi":"10.1016/j.aquatox.2025.107398","DOIUrl":"10.1016/j.aquatox.2025.107398","url":null,"abstract":"<div><div>Tebuconazole (TBZ) is a triazole fungicide broadly used to control fungal diseases in agricultural crops, fruit-bearing plants and forestry plantation<strong>s</strong>. However, its increasing use and release into aquatic environments has raised concerns about its hazardous effects on the health of fish. Thus, the aim of the present study was to review the scientific literature on the ecotoxicological effects of TBZ and TBZ-based commercial formulations on fish. Historical review data (publication year and geographical distribution), TBZ type, experimental design, fish species, habitat, life stage, tissue/organ, lethal concentration (LC₅₀), concentration and exposure time, biomarkers and effects were compiled and critically analyzed. Studies were mainly conducted with freshwater species at adult and larval stages, whereas no data were find for marine fish species. Zebrafish, (<em>Danio rerio</em>) was the most assessed species. Both TBZ and TBZ-based commercial formulations induced oxidative stress, endocrine disruption, neurotoxicity, genotoxicity, histopathologies, behavior impairments and mortality on fish. TBZ can induce synergistic and antagonistic effects on fish when it is combined to other pesticides. Overall, the current study has shown the potential hazardous effects of TBZ and TBZ-based commercial formulations on the health of fish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107398"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}