Aquatic Toxicology最新文献

{"title":"Gibberellic acid (GA) induces developmental toxicity in zebrafish (Danio rerio) embryos via oxidative stress","authors":"Ying Wei ,&nbsp;Yan Gao ,&nbsp;Sida Zhang ,&nbsp;Yue Li ,&nbsp;Zuoying Wang ,&nbsp;Xu Zhang ,&nbsp;Zan Li ,&nbsp;Jinlian Li ,&nbsp;Ying Chen ,&nbsp;Dongmei Wu","doi":"10.1016/j.aquatox.2025.107247","DOIUrl":"10.1016/j.aquatox.2025.107247","url":null,"abstract":"<div><div>Gibberellic acid (GA) is a plant growth regulator that stimulates the growth of leaves and increases yield in agricultural production. However, GA is also regarded as an environmental endocrine disruptor, and its effect on aquatic life remains unclear. In this study, the toxic effects of GA on the development of zebrafish <em>(Danio rerio)</em> embryos were evaluated, and the mechanisms were revealed. The expression of genes related to development and function in zebrafish embryos at 96 h post fertilization (96 hpf) were detected by RT-qPCR method. Furthermore, the level of reactive oxygen species (ROS) and the expression of genes related to oxidative stress were detected. The results showed that the hatching and survival rates of zebrafish embryos were inhibited by 25 and 50 μmol/L GA, and the phenotype of pericardial edema was observed, indicating that GA may have cardiotoxicity on zebrafish embryos. Further RT-qPCR experiments showed that the above results may attributed to the down-regulation of <em>Myl7</em> and <em>Vmhc</em> genes. Besides, the phenotypes of liver degeneration, and the decrease of eye size were led by 10–50 μmol/L GA, along with the alteration of <em>Fabp10a, Gclc, Gsr, Gnat1</em>, and <em>Gnat2</em> genes, suggesting that GA may exhibit toxicities on liver and eye in zebrafish embryos. In addition, the phenotype of kidney edema and the up-regulation of <em>Kim1, Plce1</em>, and <em>Pkd2</em> genes were triggered by 50 μmol/L GA, indicating that GA may have toxic effect on kidney in zebrafish embryos. The level of ROS and the expression of genes related to oxidative stress were up-regulated under 10–50 μmol/L GA exposure, which may contribute to the developmental toxicity in zebrafish embryos. In summary, GA may affect the ecological environment of aquatic life, and its harm to aquatic ecology should be given special attention in the future.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107247"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse multigeneration combined effects of nano-sized plastics and mercury on growth and reproduction in a planktonic copepod Pseudodiaptomus annandalei","authors":"Dongmei Xie ,&nbsp;Chen Zhang ,&nbsp;De-Sheng Pei","doi":"10.1016/j.aquatox.2025.107256","DOIUrl":"10.1016/j.aquatox.2025.107256","url":null,"abstract":"<div><div>Nano-plastics (NPs) and heavy metals have attracted growing scientific attention because of both pollutants’ wide distribution and ecotoxicity. However, the long-term combined toxicity of NPs and mercury (Hg) on planktonic copepods, a crucial presence in marine environments, is unknown. Here, our study aimed to investigate the multigenerational phenotypic responses of the planktonic copepod <em>Pseudodiaptomus annandalei</em> to polystyrene NPs (about 50 nm) and Hg (alone or combined) at environmentally realistic concentrations (23 μg/L for NPs and 1 μg/L for Hg), and the underlying molecular mechanisms were explored. Despite the insignificant effect on survival, NPs could threaten the development and reproduction of <em>P. annandalei</em>, being ascribed to down-regulated genes in ingestive and reproductive functions. Hg exposure revealed inhibition of reproduction probably as an energy trade-off strategy. Importantly, in combined NPs and Hg, development and reproduction were further negatively impacted, even relative to NPs or Hg alone. Correspondingly, combined NPs and Hg presented the most pronounced transcriptomic response with a series of changes in cell functions and down-regulation of key genes in the DNA replication pathway and reproductive function as compared to NPs or Hg alone. The findings indicated adverse combined effects of NPs and Hg on <em>P. annandalei</em> under multigenerational scenarios, being a greater ecological risk for planktonic copepod than NPs or Hg alone. This study provides molecular insights into the long-term toxicity of combined NPs and Hg to planktonic copepods, underlining the increased risk in the population sustainability of marine zooplankton facing co-existing plastics and Hg pollution.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107256"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute and chronic toxicity of cationic polyquaterniums of varying charge density and molecular weight to Daphnia magna and Ceriodaphnia dubia","authors":"Jessica L. Brill ,&nbsp;Anna Magdalene Brun Hansen ,&nbsp;Hope Woods ,&nbsp;Kristin A. Connors ,&nbsp;Hans Sanderson ,&nbsp;Scott E. Belanger","doi":"10.1016/j.aquatox.2024.107178","DOIUrl":"10.1016/j.aquatox.2024.107178","url":null,"abstract":"<div><div>Cationic polymers have the unique ability to neutralize negative charge with practical applications in personal care products, such as shampoos, conditioners, contact lens solutions, and as flocculants in wastewater treatment processes. Cationic polymers are a diverse class of materials varying in structural composition, cationic charge density (CD), and molecular weight (MW). In this study, we investigated three classes of polyquaternium cationic polymers (PQ-6, PQ-10, PQ-16) of varying CD and MW to characterize their toxicity to aquatic invertebrates. Although safety studies and environmental risk assessments have been conducted to support the use of polyquaterniums, further research was needed to adapt standard toxicity assays to account for the unique properties of cationic polymers. Standard acute OECD 202 assays were conducted with <em>Daphnia magna</em> and <em>Ceriodaphnia dubia</em> to explore relative species sensitivity. PQ-6 exhibited toxicity ranging from 0.09 to 2.50 mg/L, PQ-10 ranged from 21.29 to &gt;1000 mg/L, and PQ-16 ranged from 0.05 to 14.91 mg/L. Toxicity was positively correlated with CD and not correlated with MW or polymeric backbone. <em>C. dubia</em> were more sensitive to PQ exposure than <em>D. magna</em>, and this trend was consistent across all exposures. Organic carbon (humic acid and algae) was found to mitigate PQ toxicity in a dose-dependent manner, highlighting the importance of robust exposure characterization when assessing environmental risk. Chronic reproductive toxicity assays were conducted on high and low MW PQ-6 materials with observed significant decreases in days to first clutch, average offspring per clutch, and body length. Robust acute to chronic (ACR) values were derived.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107178"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity of single and binary mixtures of copper and silica nanoparticles exposed to Nitzschia closterium f. minutissima","authors":"Peining Cai,&nbsp;Qi Li,&nbsp;Shuhui Wang,&nbsp;Liju Tan,&nbsp;Jiangtao Wang","doi":"10.1016/j.aquatox.2024.107211","DOIUrl":"10.1016/j.aquatox.2024.107211","url":null,"abstract":"<div><div>A large number of nanoparticles are produced and enter the aquatic environment, where they interact with each other, posing a potential threat to aquatic organisms. The toxicity of two types of nanoparticles (nCu and nSiO₂) on <em>Nitzschia closterium f. minutissima (N. closterium f. minutissima</em>) was investigated in this study by examining changes in microalgal cell density, instantaneous fluorescence rate (Ft), and a range of antioxidant parameters in the cells. It was found that both nCu and nSiO₂ showed time- and concentration-dependent toxic effects on <em>N. closterium f. minutissima</em>. nSiO₂ could promote microalgae growth at low concentrations by providing Si, an essential element for the synthesis of siliceous shells. As the exposure time increased, both the growth and photosynthetic efficiency of the microalgae were inhibited. Nanoparticles also produced oxidative stress and caused lipid peroxidation in the microalgae. In the meantime, SOD and CAT activity were altered to protect cells from oxidative damage. Inverted biomicroscopy images showed that the microalgae enhanced their cell size to adapt to the environmental stress as exposed to 1 mg/L nCu. Scanning electron microscope (SEM) images showed that 10 mg/L nSiO₂ could adsorb nCu and reduce the toxic effect of nCu on the microalgae, while 30 mg/L nSiO₂ caused mechanical damage to microalgal cells and accelerated the internalization of nanoparticles and Cu²⁺ in the cells.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107211"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics in commercial marine bivalves: Abundance, characterization and main effects of single and combined exposure","authors":"Daniela C.C. Silva ,&nbsp;João C. Marques ,&nbsp;Ana M.M. Gonçalves","doi":"10.1016/j.aquatox.2024.107227","DOIUrl":"10.1016/j.aquatox.2024.107227","url":null,"abstract":"<div><div>Microplastics (MPs) are persistent and ubiquitous pollutants in marine ecosystems, and they can be ingested and accumulated by marine organisms with economic value to humans, such as marine bivalves, which may pose a threat to the marine food chains and to human health. In this literature review, we summarized the recent findings on the abundance and main characteristics (shape, size, color, polymer) of MPs detected in valuable marine bivalve species. Furthermore, we surveyed the major impacts triggered by MP exposure, alone or in combination with other pollutants, in these organisms. Additionally, we discussed the methodologies, techniques and equipment commonly used by researchers for the determination of the abundance, characterization and effects of the MP particles in these organisms.</div><div>We verified that MPs have been widely detected in multiple species of commercial marine bivalves, with a great variety of shapes, sizes, colors and polymer types. In general, the methodologies used by researchers to determine the MP abundance in marine bivalves need to be harmonized to facilitate the comparability between studies. So far, previous research showed that the main effects of MPs, either alone or combined with other pollutants, on commercial marine bivalves include the induction of immunological, physiological and behavioral responses, reproductive modifications, genotoxicity and neurotoxicity, which were surveyed by using a wide variety of techniques and analytical equipment. In the future, researchers should focus on less studied bivalve species and should use the most precise and innovative methodologies to assess the effects of MPs and other pollutants on marine bivalves.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107227"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The enzymatic and transcriptional adverse effects of hull in-water cleaning discharge on juvenile rockfish (Sebastes schlegeli)","authors":"Seong Hee Mun ,&nbsp;Kwang-Min Choi ,&nbsp;Dongju Shin ,&nbsp;Moonkoo Kim ,&nbsp;Taekhyun Kim ,&nbsp;Dae-Jung Kim ,&nbsp;Jee-Hyun Jung","doi":"10.1016/j.aquatox.2024.107191","DOIUrl":"10.1016/j.aquatox.2024.107191","url":null,"abstract":"<div><div>The hull in-water cleaning (IWC) process creates chemical contaminants, including antifouling paint particle mixtures that are directly discharged into the coastal environment. Recent attention has also been paid to the international regulation of ship hull cleaning discharges in environmental media. However, few studies have investigated the adverse effects or toxic pathways on resident marine species. In this study, we evaluated the chemical concentration of IWC discharge <em>in situ</em> and its toxic effects on juvenile rockfish (<em>Sebastes schlegeli</em>), a major coastal fishery resource, using enzymatic and transcriptomic studies. Zinc (8.05 ± 0.96 to 189.96 ± 47.76 μg/L) was the most abundant substance in IWC discharge, followed by copper (0.87 ± 0.19 to 1.97 ± 0.60 μg/L). No mortality was observed after 7 days of exposure in any experimental group; however, reactive oxygen species and acetylcholinesterase activity varied in juvenile rockfish exposed to the highest concentration of IWC discharge (10-fold-diluted IWC discharge). The immune and detoxification systems in juvenile rockfish exposed to IWC discharge were also significantly affected in juvenile rockfish exposed to 10-fold-diluted IWC discharge (zinc: 189.96 ± 47.76 μg/L, copper: 1.97 ± 0.60 μg/L). The expression of genes related to oxidative stress, including Cu/Zn-SOD, GST, and CAT, increased significantly in fish in all the exposure groups. Specifically, brain transcriptomic analysis revealed disturbances in the nervous system and homeostatic processes. Although lethal toxicity was not significantly affected, these findings indicate the potential hazard posed by sub-lethal concentrations of IWC discharge to juvenile fish, which are at a high-level in the food chain. Our enzymatic and transcriptomic results showed that Zn-dominant IWC discharge exposure may cause neuronal and immune toxicity in marine fish species, providing relevant insights into the management of hull IWC discharge to protect coastal ecosystems against chemical contaminants.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107191"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the regulation of cyp3a65 expression in transgenic tg(cyp3a65:GFP) zebrafish embryos","authors":"Chedi Erradhouani ,&nbsp;Benjamin Piccini ,&nbsp;Emmanuelle Maillot-Marechal ,&nbsp;Selim Aït-Aïssa ,&nbsp;Patrick Balaguer ,&nbsp;Xavier Coumoul ,&nbsp;François Brion","doi":"10.1016/j.aquatox.2025.107250","DOIUrl":"10.1016/j.aquatox.2025.107250","url":null,"abstract":"<div><div>Facing the need for alternative models allowing assessment of metabolic-endocrine disrupting chemicals (MDCs), especially in poorly investigated tissues such as the intestine, we recently developed a transgenic zebrafish embryo <em>in vivo</em> model, <em>tg(cyp3a65:GFP),</em> expressing the Green Fluorescent Protein (GFP) under the control of the zebrafish <em>cyp3a65</em> promoter, ortholog of human <em>cyp3a4</em>, a gene coding for a key enzyme of intestinal xenobiotic and endobiotic metabolism. In this study, we aimed to better understand the regulation of <em>cyp3a65</em> expression by zfPXR, zfAhR2, and zfGR zebrafish orthologs of well-known human xenosensors PXR and AhR, and steroid nuclear receptor GR. For this purpose, we performed zebrafish embryo <em>tg(cyp3a65:GFP)</em> (co)exposures to a variety of agonists (clotrimazole, TCDD, fluticasone propionate) and antagonists (econazole nitrate, CH223181, RU486), which were characterized using <em>in vitro</em> zebrafish reporter gene assays. We show that zfPXR and zfAhR2 cooperate to positively regulate <em>cyp3a65</em> expression, involving different transcription factors and their interaction. Moreover, for the first time, we show that zfGR agonist strongly inhibits the constitutive expression of <em>cyp3a65</em>, and we hypothesized the possible involvement of the transcriptional factor zfHNF4α. These results provide a better understanding of the regulation of zebrafish <em>cyp3a65</em> expression, highlighting the complex interaction between different transcription factors, which is consistent with the multiple regulatory pathways of <em>cyp3a4</em> in humans. Our data support the idea that this gene is a target of multiple contaminants capable of interacting with zfPXR, zfAhR2 and zfGR and highlights the relevance of the <em>tg(cyp3a65:GFP)</em> model to screen chemicals potentially acting as MDCs based on their modes of action at the intestinal level, which could be relevant for hazard assessment of chemicals for human and environmental health.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107250"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diving beetle, Cybister lateralimarginalis (De Geer, 1774), as a bioindicator for subcellular changes affected by heavy metal(loid) pollution in freshwater ecosystems","authors":"Dora Bjedov ,&nbsp;Nataša Turić ,&nbsp;Alma Mikuška ,&nbsp;Goran Vignjević ,&nbsp;Lucija Sara Kovačić ,&nbsp;Ana Marija Pavičić ,&nbsp;Leontina Toth Jakeljić ,&nbsp;Mirna Velki","doi":"10.1016/j.aquatox.2025.107258","DOIUrl":"10.1016/j.aquatox.2025.107258","url":null,"abstract":"<div><div>The present study assessed <em>Cybister lateralimarginalis</em> as a model species for ecotoxicological studies of metal(loid) bioaccumulation in freshwater ecosystems, by optimising protocols and analysing biomarker activities and metal(loid) concentrations from different sites and investigating their association. To accomplish this, levels of arsenic (As), selenium (Se), cadmium (Cd), mercury (Hg), and lead (Pb) were analysed, as well as a set of biomarkers were evaluated, esterase activity and oxidative stress biomarkers: fluorescence- and glutathione-based. <em>C. lateralimarginalis</em> were sampled in the spring/summer of 2023 along the Sava, Drava and Danube floodplains in Croatia. <em>C. lateralimarginalis</em> samples from Sava floodplain (Mužilovčica) showed altered carboxylesterase (CES) activity, lower glutathione (GSH) levels, and elevated Hg and As concentrations. The Weighted Pollution Index (WPI) indicated moderate pollution at the Sava floodplain, possibly due to agricultural or petrochemical impacts. At the Drava floodplain (Podravlje), higher glutathione S-transferase (GST) activity and elevated Cd, Hg, and As concentrations were observed, likely from soil accumulation, while Stara Drava beetles had lower GSH levels, suggesting agricultural impacts. Despite this, the Drava floodplain showed the lowest WPI. Higher GST activity was noted at the Danube floodplain (Kopački rit and Topoljski Dunavac), with reactive oxygen species (ROS) concentrations (Kopački rit and Podunavlje) possibly reflecting agricultural practices or illegal hunting. The present research successfully applied <em>C. lateralimarginalis</em> as a bioindicator species for assessing metal(loid)s in different environments, highlighting its reliability as an abundant apex predator, emphasizing the significance of integrating biomarker response evaluation into pollutant monitoring for efficient environmental risk assessment in freshwater ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107258"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perfluorooctanoate and nano-titanium dioxide modulate male gonadal function in the mussel Mytilus coruscus","authors":"Bingyan Sun ,&nbsp;Yiting Pan ,&nbsp;Inna Sokolova ,&nbsp;Ying Shao ,&nbsp;Menghong Hu ,&nbsp;Youji Wang","doi":"10.1016/j.aquatox.2025.107251","DOIUrl":"10.1016/j.aquatox.2025.107251","url":null,"abstract":"<div><div>Perfluorooctanoic acid (PFOA) and nano-titanium dioxide (nano-TiO₂) are widely used in industrial applications such as manufacturing and textiles, and can be released into the environment, causing toxicity to marine organisms. To study the effects of these pollutants on the gonadal development, we exposed the males of <em>Mytilus coruscus</em> to varying PFOA concentrations (2 and 200 μg/L) alone or combined with nano-TiO₂ (0.1 mg/L, size: 25 nm) for 14 days. Co-exposure to PFOA and nano-TiO₂ resulted in a short-term (7 days) decrease in the gonadosomatic index (GSI), which recovered to baseline levels. In contrast, long-term (14 days) exposure induced changes in the testes, including increased protein content, decreased lipid content, reductions in spermatic area and sperm count, and elevated apoptotic cell levels. Furthermore, key genes essential for gonadal maturation were significantly upregulated after long-term exposure. PFOA and nano-TiO₂ can disrupt the gonadal function in the male mussels by interfering with Wnt family signaling pathways, modulation of steroid and lipid metabolism and induction of apoptosis. Therefore, PFOA and nanoparticle pollutants may pose a significant risk to the reproductive capacity of mussels’ populations from polluted coastal environments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107251"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The detrimental effects and mechanisms of Orlistat in disrupting energy homeostasis and reproduction in Daphnia magna","authors":"Zhihua Ni ,&nbsp;Xinling Tian ,&nbsp;Wenbo Zhao ,&nbsp;Wenkai Hu ,&nbsp;Jinghua Lv ,&nbsp;Xiaoli Sun ,&nbsp;Yajie Zhang ,&nbsp;Yiwen Zhang ,&nbsp;Yuming Zhang ,&nbsp;Baoku Li ,&nbsp;Fengsong Liu","doi":"10.1016/j.aquatox.2024.107201","DOIUrl":"10.1016/j.aquatox.2024.107201","url":null,"abstract":"<div><div>Orlistat (ORL) has been employed as an anti-obesity pharmaceutical for several decades. Given its low absorption rate, the majority of administered ORL is excreted into the environment with feces. It is crucial to collect scientific information regarding the possible ecological risks associated with ORL. Here, the effects of ORL on <em>Daphnia magna</em> were evaluated using a 21-day chronic test at concentrations of 1, 10, 100, and 1000 μg/L. We found the inhibition of feeding and swimming activities in the 100 and 1000 μg/L ORL exposed <em>D. magna</em>, respectively. Their digestive enzyme activities and metabolites were reduced even at 1 μg/L ORL exposure. It is noteworthy that exposure to 100 μg/L ORL induced a decrease in the reproductive capacity of <em>D. magna</em>, although no discernible genotoxicity was observed. To identify the toxicological mechanisms of ORL, a metabolic analysis was conducted on <em>D. magna</em> exposed to 1000 μg/L ORL. A comprehensive reduction in carbohydrates, lipids, and amino acids was observed, which resulted in a blockage of metabolic flux towards the TCA cycle, as evidenced by mitochondrial dysfunction. These findings substantiate the detrimental impact of ORL on <em>D. magna</em> and provide insights into the underlying molecular mechanisms from a metabolic perspective.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107201"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}