Aquatic Toxicology最新文献

{"title":"Polystyrene microplastics induce molecular toxicity in Simocephalus vetulus: A transcriptome and intestinal microorganism analysis","authors":"Chenxi Zhu ,&nbsp;Hui Zhou ,&nbsp;Mengyu Bao ,&nbsp;Shengkai Tang ,&nbsp;Xiankun Gu ,&nbsp;Mingming Han ,&nbsp;Peng Li ,&nbsp;Qichen Jiang","doi":"10.1016/j.aquatox.2024.107046","DOIUrl":"10.1016/j.aquatox.2024.107046","url":null,"abstract":"<div><p>The global prevalence and accumulation of plastic waste is leading to pollution levels that cause significant damage to ecosystems and ecological security. Exposure to two concentrations (1 and 5 mg/L) of 500 nm polystyrene (PS)-nanoplastics (NPs) for 14 d was evaluated in <em>Simocephalus vetulus</em> using transcriptome and 16 s rRNA sequencing analyses. PS-NP exposure resulted in stress-induced antioxidant defense, disturbed energy metabolism, and affected the FoxO signaling pathway, causing neurotoxicity. The expression of Cyclin D1 (CCND), glucose-6-phosphatase (G6PC) and phosphoenolpyruvate carboxykinase (PCK) genes was decreased compared to the control, whereas the expression of caspase3 (CASP3), caspase7 (CASP7), Superoxide dismutase (SOD), Heat shock protein 70 (HSP70), MPV17, and Glutathione S-transferase (GST) genes was increased, thus, suggesting that NP ingestion triggered oxidative stress and disrupted energy metabolism.. PS-NPs were present in the digestive tract of <em>S. vetulus</em> after 14 days of exposure. In addition, the abundance of the Proteobacteria and opportunistic pathogens was elevated after PS-NPs exposure. The diversity and homeostasis of the <em>S. vetulus</em> gut microbiota were disrupted and the stability of intestinal barrier function was impaired. Multiomic analyses highlighted the molecular toxicity and microbial changes in <em>S. vetulus</em> after exposure to NPs, providing an overview of how plastic pollution affects freshwater organisms and ecosystems.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"275 ","pages":"Article 107046"},"PeriodicalIF":4.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142083318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microglial activation and pyroptosis induced by nano-TiO2 in marine medaka brain","authors":"Zirun Li ,&nbsp;Tao Xu ,&nbsp;Haijin Chen ,&nbsp;Xiaojie Wang","doi":"10.1016/j.aquatox.2024.107034","DOIUrl":"10.1016/j.aquatox.2024.107034","url":null,"abstract":"<div><p>Recently, nano-titanium dioxide (nano-TiO₂) has been widely distributed over surface water. However, there are few reports on its effects on the central nervous system of fish. In this study, we investigated whether nano-TiO₂ enters the medaka brain after exposure and its effect on the brain. Marine medaka brains were examined after exposure to 0.01 g/L nano-TiO₂ for 3, 10, and 20 d. Nano-TiO₂-like particles were found in the telencephalon of treated fish. There was no obvious brain histopathological injury. The number of irregular mitochondria with absent cristae increased. Gene expression of the apoptosis-related genes, <em>casp8, bcl2b</em>, and <em>bax</em>, decreased significantly in the nano-TiO₂ group at 3 d. In contrast, the pyroptosis-related genes, <em>gsdmeb</em> and <em>casp1</em>, and inflammation-related factor, <em>il18</em>, increased significantly. As an activated microglia marker, mRNA expression of <em>cd68</em> increased significantly in the nano-TiO₂ treated group. Moreover, CD68 protein expression also increased significantly at 10 d. Altogether, we show that nano-TiO₂ can alter mitochondrial morphology in the telencephalon of medaka, leading to microglial activation and pyroptosis.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"274 ","pages":"Article 107034"},"PeriodicalIF":4.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142007969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential toxic effects of nano-titanium dioxide on clams (Meretrix meretrix) with various individuality","authors":"Xiaoxue Li ,&nbsp;Erzhou Yao ,&nbsp;Jie Li ,&nbsp;Weiqun Lu","doi":"10.1016/j.aquatox.2024.107045","DOIUrl":"10.1016/j.aquatox.2024.107045","url":null,"abstract":"<div><p>Nano-TiO₂ is inevitably released into aquatic environment with increasing of nanotechnology industries. Study pointed that different individuality showed divergent behavioral and physiological response when facing environmental stress. However, the effects of nano-TiO₂ on tolerance of bivalves with different individualities remain unknown. In the study, clams were divided into two types of individuality - proactive and reactive by post-stress recovery method. It turned out that proactive individuals had quicker shell opening level, stronger burrowing behavior, faster feeding recovery, higher standard metabolic rate and more rapid ammonia excretion ability than reactive individuals after exposed to air. Then, the survival rate, hemocytes response and oxidase activity of classified clams were evaluated after nano-TiO₂ exposure. Results showed that after 30 d exposure, proactive individuals accelerated burrowing behavior with higher survival rate. Moreover, proactive clams had better adaptability and less hemocytes response and oxidative damage than reactive clams. The study highlights the individualities of marine shell fish determine individual capacity to adapt to environmental changes, play important roles in aquaculture and coastal ecosystem health.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"274 ","pages":"Article 107045"},"PeriodicalIF":4.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141979316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanism of intestinal injury induced by Bisphenol S in freshwater crayfish (Procambarus clarkii): Molecular and biochemical approaches","authors":"Changchang Pu ,&nbsp;Yuanyi Liu ,&nbsp;Lu Wang ,&nbsp;Jianshuang Ma ,&nbsp;Haolei Lv ,&nbsp;Jianyong Song ,&nbsp;Bingke Wang ,&nbsp;Aimin Wang ,&nbsp;Aimin Zhu ,&nbsp;Peng Shao ,&nbsp;Chunnuan Zhang","doi":"10.1016/j.aquatox.2024.107035","DOIUrl":"10.1016/j.aquatox.2024.107035","url":null,"abstract":"<div><p>Bisphenol S (BPS) is extensively utilized in various industries such as plastic manufacturing, food packaging, and electronics. The release of BPS into aquatic environments has been observed to have negative impacts on aquatic ecosystems. Research has shown that exposure to BPS can have adverse effects on the health of aquatic animals. This study aimed to explore the mechanism of oxidative stress and endoplasmic reticulum stress induced in freshwater crayfish (<em>Procambarus clarkii</em>) by exposure to BPS (0 µg/L, 1 µg/L, 10 µg/L, and 100 µg/L) for 14 days. The results showed that BPS exposure resulted in elevated levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and severe intestinal histological damage. In addition, oxidative stress can occur in the body by inhibiting the activity of antioxidant enzymes and the expression of related genes. BPS exposure induced a significant increase in the relative mRNA expression levels of inflammatory cytokines (<em>NF-κB</em> and <em>TNF-α</em>) and key unfolded protein response (UPR) related genes (<em>Bip, Ire1</em>, and <em>Xbp1</em>). At the same time, BPS exposure also induced up-regulation of apoptosis genes (<em>Cytc</em> and <em>Casp3</em>), suggesting that UPR and <em>Nrf2-Keap1</em> signaling pathways may play a protective role in the process of apoptosis and oxidative stress. In conclusion, Our findings present the initial evidence that exposure to environmentally relevant levels of BPS can lead to intestinal injury through various pathways, highlighting concerns about the potential harm at a population level from BPS and other bisphenol analogs.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"274 ","pages":"Article 107035"},"PeriodicalIF":4.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cadmium impacts on calcium mineralization of zebrafish skeletal development and behavioral impairment","authors":"Jingyi Hu ,&nbsp;Wen-Xiong Wang","doi":"10.1016/j.aquatox.2024.107033","DOIUrl":"10.1016/j.aquatox.2024.107033","url":null,"abstract":"<div><p>Cadmium (Cd) poses significant risks to aquatic organisms due to its toxicity and ability to disrupt the cellular processes. Given the similar atomic radius of Cd and calcium (Ca), Cd may potentially affect the Ca homeostasis, which can lead to impaired mineralization of skeletal structures and behavioral abnormalities. The formation of the spinal skeleton involves Ca transport and mineralization. In this study, we conducted an in-depth investigation on the effects of Cd at environmental concentrations on zebrafish (<em>Danio rerio</em>) skeletal development and the underlying molecular mechanisms. As the concentration of Cd increased, the accumulation of Cd in zebrafish larvae also rose, while the Ca content decreased significantly by 3.0 %−57.3 %, and vertebral deformities were observed. Transcriptomics analysis revealed that sixteen genes involved in metal absorption were affected. Exposure to 2 µg/L Cd significantly upregulated the expression of these genes, whereas exposure to 10 µg/L resulted in their downregulation. Consequently, exposure of zebrafish larvae to 10 µg/L of Cd inhibited the body segmentation growth and skeletal mineralization development by 29.1 %−56.7 %. This inhibition was evidenced by the downregulation of mineral absorption genes and decreased Ca accumulation. The findings of this study suggested that the inhibition of skeletal mineralization was likely attributed to the disruption of mineral absorption, thus providing novel insights into the mechanisms by which metal pollutants inhibit the skeletal development of fish.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"273 ","pages":"Article 107033"},"PeriodicalIF":4.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141838486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The native submerged plant, Hydrilla verticillata outperforms its exotic confamilial with exposure to polyamide microplastic pollution: Implication for wetland revegetation and potential driving mechanism","authors":"Tong Wang ,&nbsp;Xue Yang ,&nbsp;Shiyu Ouyang ,&nbsp;Wangyang Huang ,&nbsp;Guiyue Ma ,&nbsp;Shengwen Liu ,&nbsp;Yinuo Zhu ,&nbsp;Yi Zhang ,&nbsp;Haifang Li ,&nbsp;Hongwei Yu","doi":"10.1016/j.aquatox.2024.107029","DOIUrl":"10.1016/j.aquatox.2024.107029","url":null,"abstract":"<div><p>Microplastic pollution and biological invasion, as two by-products of human civilization, interfere the ecological function of aquatic ecosystem. The restoration of aquatic vegetation has been considered a practical approach to offset the deterioration of aquatic ecosystem. However, a lack of knowledge still lies in the species selection in the revegetation when confronting the interference from microplastic pollution and exotic counterpart. The present study subjected the native submerged species, <em>Hydrilla verticillata</em> and its exotic confamilial, <em>Elodea nuttallii</em> to the current and future scenarios of polyamide microplastic pollution. The plant performance proxies including biomass and ramet number were measured. We found that the native <em>H. verticillata</em> maintained its performance while the exotic <em>E. nuttallii</em> showed decreases in biomass and ramet number under severest pollution conditions. The restoration of native submerged plant such as <em>H. verticillata</em> appeared to be more effective in stabilizing aquatic vegetation in the scenario of accelerating microplastic pollution. In order to explore the underlying driving mechanism of performance differentiation, stress tolerance indicators for plants, sediment enzymatic activity and sediment fungal microbiome were investigated. We found that polyamide microplastic had weak effects on stress tolerance indicators for plants, sediment enzymatic activity and sediment fungal diversity, reflecting the decoupling between these indicators and plant performance. However, the relative abundance of sediment arbuscular mycorrhizal fungi for <em>H. verticillata</em> significantly increased while <em>E. nuttallii</em> gathered “useless” ectomycorrhizal fungi at the presence of severest polyamide microplastic pollution. We speculate that the arbuscular mycorrhizal fungi assisted the stabilization of plant performance for <em>H. verticillata</em> with exposure to the severest polyamide microplastic pollution.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"273 ","pages":"Article 107029"},"PeriodicalIF":4.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the ototoxic effects of paraquat on zebrafish larva","authors":"M.S. Ananthakrishna Tantry,&nbsp;Kirankumar Santhakumar","doi":"10.1016/j.aquatox.2024.107030","DOIUrl":"10.1016/j.aquatox.2024.107030","url":null,"abstract":"<div><p>Paraquat is a widely utilized herbicide in agricultural fields posing a significant impact on human health and the environment due to its potent oxidant properties. Rampant paraquat usage leads to serious health hazards to farmers and the ecosystem, particularly the water bodies. Paraquat exposure can damage dopaminergic neurons causing Parkinson's disease in humans and other animal models. Extensive research has been done regarding the mode of action, pathophysiology and molecular mechanisms of paraquat-induced Parkinson's disease. Meanwhile, the ototoxic effect of paraquat remains poorly understood. Potential ototoxins can cause sensorineural hearing loss, one of the most common sensory disabilities in humans. In this study, we investigated the harmful effects of paraquat on neuromast hair cells in zebrafish larvae, a powerful model organism for auditory research. We treated sub-lethal concentrations (125 μM to 1000 μM) of paraquat to 3 and 4 dpf zebrafish larvae to investigate its ototoxic effects via rheotaxis behavioral assay, neuromast staining and scanning electron microscopy. The behavioral assay findings showed a drastic decline in the rheotaxis behavior in all the concentrations of paraquat-treated larvae. Furthermore, DASPEI neuromast vital staining displayed a dose-dependent reduction in the neuromast hair cells as we increased the paraquat concentration. The scanning electron microscope data revealed the significant shortening of kinociliary length, a decrease in stereociliary density and changes in semilunar peridermal cell morphology signifying the damaging effects of paraquat at the cellular level. Collectively, the behavioral, anatomical and morphological studies highlight the potential ototoxic effects of paraquat on zebrafish neuromast hair cells, further signifying its potential role in causing hearing loss in humans.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"273 ","pages":"Article 107030"},"PeriodicalIF":4.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do DOM quality and origin affect the uptake and accumulation of a lipid-soluble contaminant in a filter feeding ascidian species (Ciona) that can target small particle size classes?","authors":"Sabrina Schultze ,&nbsp;Hilde K. Langva ,&nbsp;Jing Wei ,&nbsp;Marios Chatzigeorgiou ,&nbsp;Jan T. Rundberget ,&nbsp;Dag O. Hessen ,&nbsp;Anders Ruus ,&nbsp;Tom Andersen ,&nbsp;Katrine Borgå","doi":"10.1016/j.aquatox.2024.107026","DOIUrl":"10.1016/j.aquatox.2024.107026","url":null,"abstract":"<div><p>The widely reported increase of terrestrial dissolved organic matter (terrDOM) in northern latitude coastal areas (“coastal darkening”) can impact contaminant dynamics in affected systems. One potential impact is based on differences in chemical adsorption processes of the molecularly larger terrDOM compared to marine DOM (marDOM) that leads to increased emulsification of lipophilic contaminants with terrDOM. Filter feeders filter large amounts of water and DOM daily and thus are directly exposed to associated contaminants through both respiration and feeding activity. Thus, increased exposure to terrDOM could potentially lead to an increase in bioaccumulation of lipid soluble contaminants in filter feeders. To assess the effect of DOM on bioaccumulation in filter feeders, we exposed the mucous based filter feeding ascidian <em>Ciona intestinalis (</em>formerly known as <em>Ciona intestinalis Type B),</em> to the lipophilic veterinary drug teflubenzuron (log K_OW: 5.39) in combination with four DOM treatments: TerrDOM, marDOM, a mix of the two called mixDOM, and seawater without DOM addition. The exposure lasted for 15 days, after which the individuals in all DOM treatments showed a trend towards higher bioaccumulation of Teflubenzuron than those in the seawater control. However, there was considerable overlap in posterior distributions. Against our expectations, marDOM resulted in the highest bioaccumulation factor (BAF), followed by mixDOM, with terrDOM resulting in the lowest BAF except for seawater (kinetic BAF L/kg median, 2.5 %–97.5 % percentile marDOM 94, 74–118; mixDOM 82, 63–104; terrDOM 79; 61-99; seawater 61, 44–79). All BAFs were below the level of concern according to the EU REACH regulation (BAF &lt; 2000 L / kg) and, therefore, likely not environmentally problematic in the examined context. However, the results show that DOM can act as a dietary vector; thus, different combinations of contaminants, DOM, and filter feeding organisms should be tested further.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"273 ","pages":"Article 107026"},"PeriodicalIF":4.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0166445X24001966/pdfft?md5=607a7770aac54e21673551a36d927de6&pid=1-s2.0-S0166445X24001966-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141699261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lower concentration polyethylene microplastics can influence free-floating macrophyte interactions by combined effects of many weak interactions: A nonnegligible ecological impact","authors":"Hui Yang ,&nbsp;Hongbo Zhao ,&nbsp;Hongzhi Mao ,&nbsp;Yunhai Pu ,&nbsp;Qiutong Peng ,&nbsp;Zhiyan Xu ,&nbsp;Xu Zhang ,&nbsp;Feng Huang ,&nbsp;Zhongqiang Li","doi":"10.1016/j.aquatox.2024.107028","DOIUrl":"10.1016/j.aquatox.2024.107028","url":null,"abstract":"<div><p>Microplastics (MPs) are ubiquitous in freshwater ecosystems and their accumulation has been considered an emerging threat. Early research on the effects of MPs on macrophytes primarily focused on the toxicological impacts on individual macrophytes, with several studies suggesting that lower concentrations of MPs have little impact on macrophytes. However, the ecological implications of lower MP concentrations on macrophyte communities remain largely unexplored. Here, we experimented to assess the effects of lower concentrations including 25 mg/L, 50 mg/L, 75 mg/L, and 100 mg/L of polyethylene (PE) microplastics on <em>Spirodela polyrhiza</em> and <em>Lemna minor</em>, and their community. Our results also indicated that PE concentrations below 100 mg/L had no significant effect on relative growth rate, specific leaf area, Chlorophyll a, Chlorophyll <em>b</em>, Chlorophyll <em>a</em> + <em>b</em>, carotenoid, malondialdehyde (MDA), catalase, and soluble sugar of monocultural <em>S. polyrhiza</em>. However, a lower concentration of PE significantly decreased the MDA of monocultural <em>L. minor</em> and significantly affected the comprehensive index of <em>S. polyrhiza</em>. These findings suggested that lower concentrations of PE can influence interactions between macrophytes maybe due to the cumulative effects of many weak interactions. Additionally, our study showed that 75 mg/L and 100 mg/L PE additions decreased the competitive balance index value of two macrophytes under mixed-culture condition. This result implied that the ecological influence of lower concentration MPs on macrophytes may manifest at the community level rather than at the population level, due to species-specific responses and varying degrees of sensitivity of macrophytes to PE concentrations. Thus, our study emphasizes the need to closely monitor the ecological consequences of emerging contaminants such as MPs accumulation on macrophyte communities, rather than focusing solely on the morphology and physiology of individual macrophytes.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"273 ","pages":"Article 107028"},"PeriodicalIF":4.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Female zebrafish (Danio rerio) exposure to polystyrene nanoplastics induces reproductive toxicity in mother and their offspring","authors":"Chunyun Zhang ,&nbsp;Luoxin Li ,&nbsp;Juan Jose Alava ,&nbsp;Zebang Yan ,&nbsp;Peng Chen ,&nbsp;Yasmeen Gul ,&nbsp;Lixin Wang ,&nbsp;Dongmei Xiong","doi":"10.1016/j.aquatox.2024.107023","DOIUrl":"10.1016/j.aquatox.2024.107023","url":null,"abstract":"<div><p>Nanoplastics (NPs) have been commonly detected in aquatic ecosystems, and their negative effects on aquatic organisms have raised concerns in the scientific community and general public. The acute toxicity, neurotoxicity, and metabolic toxicity induced by NPs on fishes have been reported by many studies, although less attention has been focused on how mother exposed to NPs affected their offspring in aquatic organisms. Here, female zebrafish (F0) were exposed to 0, 200 and 2000 μg/L polystyrene nanoplastics (PS-NPs) for 42 d, with their offspring (F1) reared in clear water until sexual maturity. The results showed that PS-NPs were detected in various organs of F0 and F1. PS-NPs exposure significantly decreased gonadal 17-estradiol (E2), while increasing testosterone (T) contents. Lower levels of <em>cyp19a1a, lhr</em> and <em>erα</em> expressions in the 2000 μg/L group were consistent with a reduced number of mature oocytes (MO), but an increase in perinucleolar oocytes (PO). Interestingly, the expression of <em>vtg</em> was only up-regulated by 200 μg/L PS-NPs. After exposure, the egg production was dramatically reduced, but the hatching rate and heartbeat of F1 embryos from treated females were significantly higher than those observed in females from the control group. Maternal PS-NPs exposure significantly decreased the E2 and T levels in F1 adults, while PS-NPs exposure significantly up-regulated the <em>sox9a</em> but down-regulated the <em>foxl2a</em> in F1 larvae of 30 days post fertilization (dpf). This study showed that PS-NPs caused reproductive toxicity by changing the hypothalamic-pituitary-gonadal (HPG) axis-related genes, impairing the reproductive capacity of female zebrafish, affecting the development and disrupting the endocrine function of F1. These results suggested that PS-NPs had adverse effects on fish reproductive system both in the directly exposed generation and in their unexposed offspring.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"273 ","pages":"Article 107023"},"PeriodicalIF":4.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141711436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}