Aquatic Toxicology最新文献

{"title":"Antibacterials exert toxic effects on aquatic organisms by inhibiting respiration, inducing oxidative stress, mitochondrial dysfunction and autophagy","authors":"Hagar Fadda ,&nbsp;Raheema Hassan Khan ,&nbsp;Yara Shqair ,&nbsp;Cemile Uslu ,&nbsp;Alexander V. Panov ,&nbsp;Alex Lyakhovich","doi":"10.1016/j.aquatox.2025.107284","DOIUrl":"10.1016/j.aquatox.2025.107284","url":null,"abstract":"<div><div>Although bactericidal antibiotics are an integral part of infectious disease medicine, the uncontrolled use of these drugs in recent years is beginning to affect the environment, especially water resources. With the exception of a few well-documented toxic effects, antimicrobials are thought to act mainly on bacteria and, at low doses, have no deleterious effects on eukaryotic organisms. However, since mitochondria are direct ancient relatives of prokaryotes, mitochondrial dysfunction induced by antibiotic exposure can also occur in higher eukaryotes. In this work, we used the microcrustacean <em>Artemia salina</em> to show how some known antibacterial drugs exert toxic effects on mitochondria, suppressing cellular and organismal respiration in aquatic organisms. We have demonstrated that exposure to antimicrobials of different classes, namely kanamycin A, tetracycline hydrochloride, erythromycin, and chloramphenicol, leads to the accumulation of reactive oxygen species and is accompanied by autophagy. Our results suggest that the emergence of antibactericidal drugs, increasingly appearing in wastewater, may have far-reaching consequences for aquatic areas adjacent to large cities.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107284"},"PeriodicalIF":4.1,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticidal nanoformulations based on a lipid matrix: Physicochemical properties and their effect on non-target aquatic biota","authors":"Catarina Ganilho ,&nbsp;Manuel A. Andino-Enríquez ,&nbsp;Thacilla I. de Menezes ,&nbsp;Lisete Fernandes ,&nbsp;Carlos M. Pereira ,&nbsp;Ruth Pereira ,&nbsp;Tatiana Andreani","doi":"10.1016/j.aquatox.2025.107268","DOIUrl":"10.1016/j.aquatox.2025.107268","url":null,"abstract":"<div><div>The incorporation of lambda-cyhalothrin (LC) in lipid nanoparticles (LN) could be a sustainable strategy to increase its efficacy and decrease its hazard to the environment. The purpose of the present work was to perform the interaction between LC and LN after nanoencapsulation and to evaluate their effect on species from different aquatic trophic levels such as <em>Aliivibrio fischeri, Raphidocelis subcapitata, Lemna minor,</em> and <em>Daphnia magna</em>. LN loaded with LC (LN-LC) were produced by green and simple methodology without organic solvents using Precirol ATO5® and Capryol 90® as solid and liquid lipids, respectively, and soy lecithin and TEGO® Care as emulsifiers. The physicochemical interaction between LC and LN was assessed by differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR), and X-ray, confirming that LC is associated with the lipid lattice of nanoparticles, characterized by an amorphous matrix. The data from biological tests showed no or low toxicity of LN-LC on the selected aquatic organisms. Thus, encapsulation in lipid-based nanoparticles may be a promising and sustainable choice for using this insecticide in agricultural practices, reducing its environmental risk.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107268"},"PeriodicalIF":4.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effect of mercury and ammonia toxicity and its mitigation through selenium nanoparticles in fish","authors":"Anupama Shinde ,&nbsp;Rajesh Sharma ,&nbsp;Prem Kumar ,&nbsp;Tarkeshwar Kumar ,&nbsp;Kotha Sammi Reddy ,&nbsp;Neeraj Kumar","doi":"10.1016/j.aquatox.2025.107270","DOIUrl":"10.1016/j.aquatox.2025.107270","url":null,"abstract":"<div><div>An experiment was conducted to mitigate mercury and ammonia toxicity (Hg + NH₃) in <em>Oreochromis niloticus</em> (GIFT strain) using selenium nanoparticles (Se-NPs). The Se-NPs were synthesized using green methods, employing fish waste as the substrate. Experimental diets were prepared by supplementing Se-NPs at 0, 0.3, and 0.6 mg kg⁻¹. The oxidative stress enzymes, including catalase (CAT) and glutathione peroxidase (GPx), in the liver and kidney tissues were significantly reduced by Se-NPs at 0.3 and 0.6 mg kg⁻¹ under Hg + NH₃ stress compared to the control and stressor in 20 and 40 day periods. Additionally, superoxide dismutase (SOD) activity in the kidney at 20 days and in the liver at 40 days was significantly reduced by supplementation of Se-NPs under similar conditions. The activity of acetylcholine esterase (AChE), was significantly inhibited by Hg + NH₃ toxicity. Whereas, AChE activity was enhanced by Se-NPs supplementation at 0.3 and 0.6 mg kg⁻¹ during 20 and 40 day intervals. The gene expression of <em>HSP70, iNOS, CYP450, Caspase-3a</em>, and <em>TNFα</em> in liver tissue, and <em>MYST</em> in muscle tissue was upregulated by Hg+NH₃ toxicity. However, this upregulation was significantly downregulated by supplementation of Se-NPs at 0.3 and 0.6 mg kg⁻¹ under Hg + NH₃ stress. Moreover, immunoglobulin (<em>Ig</em>) and growth hormone (<em>GH)</em> levels were noticeably upregulated with Se-NPs compared to the control and Hg+NH₃ stress. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) in liver and gill tissues, were significantly elevated by Hg+NH₃ toxicity, were reduced by Se-NPs diet. Conversely, digestive enzyme activities, including protease, amylase, and lipase, were significantly enhanced by Se-NPs under stress conditions. Dietary supplementation with Se-NPs at 0.3 and 0.6 mg kg⁻¹ improved growth performance parameters such as final weight gain percentage, feed conversion ratio, protein conversion ratio, specific growth rate, daily growth index, and relative feed intake compared to the control and other groups. DNA damage, assessed in terms of tail DNA percentage, was significantly reduced with Se-NPs supplementation. Additionally, mercury detoxification was significantly enhanced with Se-NPs-supplemented diets. In conclusion, this study demonstrates that dietary Se-NPs effectively alleviate the adverse effects of mercury and ammonia toxicity by modulating antioxidant status, enhancing immunomodulation, and mitigating stress biomarker impacts through changes in gene expression in fish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107270"},"PeriodicalIF":4.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolonged curcumin supplementation causes tissue-specific antioxidant responses in adult oysters: Potential implications for resilience against abiotic and biotic stressors in the aquaculture industry","authors":"Heloísa Bárbara Gabe ,&nbsp;Karine Amabile Taruhn ,&nbsp;Danielle Ferraz Mello ,&nbsp;Melody Lebrun ,&nbsp;Christine Paillard ,&nbsp;Charlotte Corporeau ,&nbsp;Alcir Luiz Dafre ,&nbsp;Rafael Trevisan","doi":"10.1016/j.aquatox.2025.107282","DOIUrl":"10.1016/j.aquatox.2025.107282","url":null,"abstract":"<div><div>Aquatic animals inhabiting marine coastal environments are highly susceptible to environmental fluctuations and pollution, exemplified by widespread mass mortalities induced by marine bacteria or viruses. Enhancing antioxidant defenses presents a promising strategy to mitigate such environmental stressors. We postulated that supplementation of oysters with natural compounds such as flavonoids, exemplified by curcumin (CUR), could effectively bolster their antioxidant protection. Adult Pacific oysters were supplemented with CUR (30 μM) in seawater for 2, 4, 8, and 16 days. CUR metabolites progressively accumulated in gills, mantle, and digestive glands. Notably, oyster antioxidant response was significantly augmented, as evidenced by elevated glutathione (GSH) levels, and enhanced activities of glutathione reductase (GR), thioredoxin reductase (TrxR), and glutathione S-transferase (GST) after 4, 8, and 16 days of CUR supplementation. This response was tissue-specific, with the most pronounced increase in gills, followed by mantle, whereas digestive gland exhibited minimal response. After being supplemented with CUR for 8 days, oysters were subjected to antioxidant-disrupting agents such as N-ethylmaleimide (NEM), 1‑chloro-2,4-dinitrobenzene (CDNB). Both chemicals reduced antioxidant protection in untreated animals. However, CUR supplementation prevented these redox-disrupting effects, suggesting the potential ability of CUR to counteract antioxidant stressors. The effects of 8 days of CUR supplementation were also tested against the lethal effects of the pathogens V<em>. tapetis,</em> V<em>, alginolyticus,</em> and V<em>. anguillarum,</em> but CUR failed to induce immunological protection<em>.</em> The antioxidant protection induced by CUR holds promise for application in aquaculture to bolster animal health and resilience against abiotic stressors. Further research is needed to investigate the long-term impact of CUR supplementation and its role against biotic stressors, such as bacterial and viral infections.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107282"},"PeriodicalIF":4.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of CYP1A and AhR modulation on polycyclic aromatic hydrocarbon-induced developmental defects in Japanese medaka","authors":"Shusaku Fukugami ,&nbsp;Masatoshi Yamasaki ,&nbsp;Emiko Kokushi ,&nbsp;Seiichi Uno","doi":"10.1016/j.aquatox.2025.107267","DOIUrl":"10.1016/j.aquatox.2025.107267","url":null,"abstract":"<div><div>Polycyclic aromatic hydrocarbons (PAHs) are known to induce developmental malformations in fish embryos. However, the interaction between aryl hydrocarbon receptor (AhR) and cytochrome P450 (CYP) in PAH-induced development defects remains unclear. Therefore, we investigated the effects of the CYP1A inhibitor piperonylbutoxide (PBO) and the AhR antagonist CH223191 (CH) on the development of Japanese medaka (<em>Oryzias Latipes</em>) embryos exposed to different PAHs. Japanese medaka embryos were exposed to three conditions: PAH alone, PAH and PBO, and PAH and CH. Microscopic observations were performed to examine the presence of developmental defects. Although neither phenanthrene (Phe) nor fluoranthene (Flu) induced morphological malformations in larvae, benzo(a)anthracene (BaA) exposure induced craniofacial deformities in the larvae. Additionally, BaA and PBO co-exposure significantly increased the rate and severity of malformations. Pyrene (Pyr) exposure induced craniofacial defects, cardiac hypertrophy, pericardial edema, and spinal curvature, which were attenuated by exposure to either CH or PBO. Collectively, these findings suggest that structurally different PAHs exert their toxic effects via distinct mechanisms during fish development.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107267"},"PeriodicalIF":4.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The key molecular mechanisms of antagonism induced by combined exposure to erythromycin and roxithromycin in Chlorella pyrenoidosa","authors":"Feifan Wu ,&nbsp;Xiangjie Pan ,&nbsp;Yuhao Zhou ,&nbsp;Yan Zhu ,&nbsp;Kai Liu ,&nbsp;Wei Li ,&nbsp;Jiangang Han","doi":"10.1016/j.aquatox.2025.107269","DOIUrl":"10.1016/j.aquatox.2025.107269","url":null,"abstract":"<div><div>Emerging pollutants such as antibiotics have raised great concern in recent years, but the complex coexistence of multiple antibiotics in the environment poses a new challenge in the accurate assessment of the toxicity of antibiotics to aquatic organisms such as microalgae. In this study, the mechanism of action of a combination of erythromycin (ERY) and roxithromycin (ROX) on <em>Chlorella pyrenoidosa</em> was illustrated based on the physiological–biochemical response and transcriptomic analysis. The results revealed an inhibitory effect on the biomass of <em>C. pyrenoidosa</em> at 14 d in all treatment groups, whereas an antagonistic effect was observed in the coexposure groups. The photosystem was the main target despite the existence of multiple compensatory mechanisms, such as expanding the antenna size and initiating alternative electron carriers. The intercept of electrons on the donor side of PSI limited the production of energy, whereas the adjustment of the content and ratio of pigments strengthened microalgal adaptation. Enzymes and genes related to the degradation of exogenous compounds, including cytochrome P450 (P450), glutathione S-transferase (GST) and ABC transporters, mediated the detoxification of antibiotics. The upregulated expression of related genes induced by coexposure increased resistance and explained the antagonistic effects. The shift in energy allocation by increasing the proportion of lipids met the urgent requirements of microalgal physiological activities. This study reemphasizes the modes of interactions between multiple antibiotics and provides new insights into the mechanisms of antagonism induced by combinations of antibiotics.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107269"},"PeriodicalIF":4.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin C alleviates intestinal damage induced by 17α-methyltestosterone in Carassius auratus","authors":"Haiyan Zhao ,&nbsp;Tongyao Li ,&nbsp;Chenyang Li ,&nbsp;Zijun Xiong ,&nbsp;Weiya Rong ,&nbsp;Lu Cao ,&nbsp;Gen Chen ,&nbsp;Qing Liu ,&nbsp;Yu Liu ,&nbsp;Xianzong Wang ,&nbsp;Shaozhen Liu","doi":"10.1016/j.aquatox.2025.107266","DOIUrl":"10.1016/j.aquatox.2025.107266","url":null,"abstract":"<div><div>17α-Methyltestosterone (MT), an environmental endocrine-disrupting chemical (EDC), is widely present in aquatic environments, posing potential threats to the health of aquatic organisms. This study aimed to explore the protective effects of Vitamin C (VC) against MT-induced intestinal injury in <em>Carassius auratus</em> and evaluate the optimal VC dosage. <em>C. auratus</em> were exposed to 50 ng/L MT with 0, 25, 50, and 150 mg/kg VC for 7, 14, and 21 d. Intestinal pathological changes were assessed using paraffin sections, digestive enzyme activity was measured, RT-qPCR was used to analyze the expression of genes related to the intestinal barrier and inflammation, and 16S rDNA sequencing was conducted to evaluate the intestinal flora. MT exposure caused villus damage, vacuolization, and free lymphocytes. Additionally, it upregulated <em>TNF-α</em> and <em>Claudin-12</em> expression and downregulated <em>IL-10, Occludin,</em> and <em>Muc2</em> expression, exacerbating intestinal inflammation, damaging barrier function, and reducing digestive enzyme activity. VC at 25 and 50 mg/kg significantly alleviated MT-induced damage by restoring villus length and mitigating the downregulation of anti-inflammatory factors and tight junction protein-related genes while inhibiting <em>TNF-α</em> mRNA expression. 16S rDNA sequencing revealed that MT disrupted the intestinal flora and increased the abundance of harmful bacteria such as <em>Acinetobacter</em>, whereas VC promoted <em>Lactobacillus</em> production and enhanced digestive enzyme activity. We hypothesize that MT exposure promotes the growth of harmful bacteria in the intestines, leading to inflammation and damage to the mucosal barrier. 25 and 50 mg/kg VC can mitigate MT-induced intestinal injury by regulating the intestinal microbiota and have potential protective effects<em>.</em></div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107266"},"PeriodicalIF":4.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of polymethylmethacrylate microplastics with arsenic and copper on the euryhaline rotifer Proales similis","authors":"Uriel Arreguin-Rebolledo ,&nbsp;Federico Páez-Osuna ,&nbsp;Gladys Valencia-Castañeda ,&nbsp;Mario Alberto Arzate-Cárdenas ,&nbsp;Mariana V. Capparelli","doi":"10.1016/j.aquatox.2024.107214","DOIUrl":"10.1016/j.aquatox.2024.107214","url":null,"abstract":"<div><div>Aquatic organisms are typically exposed to chemical mixtures, including microplastics and metal(loid)s. However, most research has primarily focused on the toxicity of individual chemicals, potentially overestimating their risks to aquatic life. This study examined the combined effects of polymethylmethacrylate microplastics (PMMA-MPs) with As and Cu at environmentally relevant concentrations on the euryhaline rotifer <em>Proales similis</em>. The 48-h EC₅₀ of PMMA-MPs (1.5–11.5 μm) was determined using concentrations from 1.0 to 100 mg/L. These concentrations were then combined with 0.41 mg/L As or 0.034 mg/L Cu. The combined effects of PMMA-MPs (0.031–1.0 mg/L) with As (0.025–0.2 mg/L) and Cu (0.0025–0.02 mg/L) at environmental concentrations were assessed through five-day chronic toxicity reproductive tests. The population growth rate was the endpoint for both acute and chronic testing. The EC₅₀ of PMMA-MPs for <em>P. similis</em> was 44.0 mg/L, but this value significantly decreased when PMMA-MPs were co-exposed with the tested metal(loid)s. The tested mixtures of PMMA-MPs with As and Cu reduced growth rates by 13 – 48 % and 11 – 35 %, respectively, compared to the individual exposure to each chemical. This indicates that PMMA-MPs alter the toxic behavior of these metal(loid)s and vice versa. The present findings reveal that the combination of environmentally relevant concentrations of MPs (modeled as PMMA-MPs) with As and Cu can induce synergistic effects in marine rotifers. These results highlight the importance of investigating pollution in realistic scenarios that many aquatic invertebrates face.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107214"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological and histochemical analysis of the digestive tract of adult horseshoe crabs under mercury stress","authors":"Wanling Zhang ,&nbsp;Mujiao Xie ,&nbsp;Xiaohai Chen ,&nbsp;Ziwei Ying ,&nbsp;Yuyuan Bao ,&nbsp;Yang-Guang Gu ,&nbsp;Xiaoyong Xie","doi":"10.1016/j.aquatox.2024.107202","DOIUrl":"10.1016/j.aquatox.2024.107202","url":null,"abstract":"<div><div>Mercury (Hg) contamination is a major environmental concern that continuously impacts marine organisms, including the mangrove horseshoe crab (<em>Carcinoscorpius rotundicauda</em>). As a vulnerable species, <em>C. rotundicauda</em> holds significant ecological and evolutionary value, making it particularly susceptible to Hg pollution and deserving of focused conservation efforts. This study presents the first comprehensive histopathological and histochemical analysis of mercury toxicity in the digestive tract of adult <em>C. rotundicauda</em>. Through both qualitative and quantitative analyses of histopathological and histochemical alterations, we evaluated the effects of acute Hg exposure (0.5 mg/L and 5 mg/L) on the esophagus, stomach, and intestines over time. The results revealed significant dose- and time-dependent tissue damage, with the stomach exhibiting the most pronounced alterations, including epithelial detachment, vacuolation caused by fibers degradation in the loose connective tissue, and muscle layer disruption, followed by the esophagus and intestines. Additionally, mucous cell density in the hindgut submucosa decreased by 30 % after 96 h of acute Hg exposure (0.5 and 5 mg/L HgCl₂), indicating a weakened protective barrier. The tissue damage of the digestive tract may further have a negative impact on the health of the adult horseshoe crab, thus threatening the survival of <em>C. rotundicauda</em> population and reducing the biodiversity of the mangrove ecosystem. These findings underscore the critical threat posed by Hg pollution to the digestive system of <em>C. rotundicauda</em> and offer novel insights into the toxicological impact on marine invertebrates. Considering the tissue-damaging effects of Hg on adult horseshoe crabs, this study underscores the importance of regular monitoring of Hg contamination in natural habitats. The results offer valuable guidance for future habitat restoration and effective management of existing habitats.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107202"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polystyrene microplastics exacerbate acetochlor-induced reproductive toxicity and transgenerational effects in zebrafish","authors":"Yang Wang ,&nbsp;Chaofan Ma ,&nbsp;Zenglong Chen ,&nbsp;Yinping Guo ,&nbsp;Yuan Jing ,&nbsp;Xiaolei Chen ,&nbsp;Hongwu Liang","doi":"10.1016/j.aquatox.2024.107208","DOIUrl":"10.1016/j.aquatox.2024.107208","url":null,"abstract":"<div><div>Microplastic (MPs) can adsorb co-existing pollutants, and alter their behavior and toxicity. Meanwhile, amide herbicides like acetochlor (ACT) are widely used in agriculture, with potential endocrine-disrupting effects that raise ecological concerns. The aim of this research was to examine the effects of MPs on the reproductive endocrine disruption caused by ACT and the effects of maternal transmission. Zebrafish were employed in this study to assess the reproductive toxicity of ACT alone and in combination with polystyrene microplastics (PS-MPs) of different sizes (200 nm and 2 μm) and concentrations (0.1 and 1 mg/L) over a 63-day exposure experiment. The results indicated that ACT was concentrated in zebrafish tissues in the order: intestine &gt; liver &gt; gill &gt; brain &gt; gonad &gt; muscle. PS-MPs increased ACT bioaccumulation, worsened gonadal damage, led to abnormalities in hormone levels, and caused disruptions in HPG axis gene expression, further exacerbating the reproductive toxicity. Maternal transfer of ACT affected offspring growth, thyroid function, and HPT axis gene expression, with nanoplastics (NPS) amplifying these adverse effects. This study offers crucial insights into the ecological hazards posed by ACT and PS-MPs, emphasizing the increased toxicity due to PS-MPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107208"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}