Aquatic Toxicology最新文献

{"title":"Metabolomics approach to evaluate diclazuril-induced developmental toxicity in zebrafish embryo","authors":"Veena Jain ,&nbsp;Ved Prakash ,&nbsp;Garima Sagar ,&nbsp;Alok Kumar ,&nbsp;Pankaj Ramji Jagdale ,&nbsp;Anjaneya Ayanur ,&nbsp;Sadasivam Anbumani ,&nbsp;Somendu Kumar Roy","doi":"10.1016/j.aquatox.2025.107238","DOIUrl":"10.1016/j.aquatox.2025.107238","url":null,"abstract":"<div><div>Anticoccidials, commonly used in veterinary medicine to treat coccidiosis in food-producing animals, particularly in poultry farming, are associated with potential environmental risks due to their excretion in manure and subsequent land-spreading. Diclazuril, a widely used anticoccidial, has been detected in groundwater, raising concerns about its impact on non-target species. This study investigates the developmental toxicity of diclazuril in zebrafish embryos over a 96-hour exposure period, utilizing biomarkers such as oxidative stress indicators and metabolomic profiles. The acute toxicity assessment determined an LC₅₀ of 255 µg/L for diclazuril. Observed sublethal effects included pericardial edema, curved spine, and yolk sac edema, which worsened with increasing concentrations from 106 µg/L to 515 µg/L. Based on the Lowest Observed Adverse Effect Level (LOAEL), further experiments were conducted at concentrations of 50 µg/L, 100 µg/L, and 200 µg/L. Significant increases in reactive oxygen species (ROS) were noted at 100 µg/L and 200 µg/L, alongside notable reduction in superoxide dismutase (SOD) and glutathione S-transferase (GST) activities at concentrations ≥100 µg/L, while no significant changes observed in catalase (CAT) activity. Metabolomic analysis using GC–MS/MS revealed significant disturbances in pathways such as pyruvate metabolism, the citric acid cycle, and amino acid metabolism, indicating potential mitochondrial dysfunction in groups exposed to concentrations ≥100 µg/L. Furthermore, alterations in histological lesions in brain region and altered neurotransmitter activity suggests possible neurobehavioral disorders. Increased oxidative stress, along with decreased ATP and NADH levels, points to mitochondrial dysfunction, which is further supported by ultrastructural analysis and locomotor behavior confirming mitochondrial disruption. The disruption of cellular energetics is likely a key factor contributing to the neurotoxic effects observed in zebrafish embryos exposed to ≥100 µg/L of diclazuril.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107238"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ingestion of polyethylene microplastics impacts cichlid behaviour despite having low retention time","authors":"Miguel Eduardo L. Felismino,&nbsp;Ségolène Chevallier Rufigny,&nbsp;Samuel Evan Gonzalez-Fleurant,&nbsp;Grant E. Brown","doi":"10.1016/j.aquatox.2025.107248","DOIUrl":"10.1016/j.aquatox.2025.107248","url":null,"abstract":"<div><div>Microplastics, particles between 0.001 and 5 mm in diameter, are ubiquitous in the environment and their consumption by aquatic organisms is known to lead to a variety of adverse effects. However, studies on the effects of microplastics on prey fish have not shown consistent trends, with results varying across species and plastic type used. Here, we manipulated the levels of microplastic (MP) exposure among juvenile convict cichlids (<em>Archocentrus nigrofasciatus</em>) by feeding them brine shrimp (<em>Artemia spp</em>.) exposed to 0, 10, or 100 MP ml^-1 of virgin polyethylene microspheres (10–20 μm) for a 10-day period. We then tested groups of 3 cichlids in a 2-day maze trial, in which we measured the latency to explore and time to complete a novel maze. We found no impacts of microplastic exposure on foraging rate, growth, or competitive aggression. However, our results demonstrate that microplastics exposure shaped exploratory behaviour and maze performance. Despite these effects, we found very little microplastics remaining in the fish's bodies after the experiment. A companion experiment demonstrates that most plastic particles were egested within 24 h. Our current results show that pristine microplastics at non-lethal levels have consequences on cichlid behaviour and decision-making but not growth.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107248"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Size-specific mediation of the physiological responses and degradation ability of microalgae to sulfamerazine by microplastics","authors":"Xinlei Wang ,&nbsp;Min Lv ,&nbsp;Jin Liu ,&nbsp;Mingtao Ba ,&nbsp;Mingsan Man ,&nbsp;Kun Yin ,&nbsp;Jing Ding ,&nbsp;Xianbo Chang ,&nbsp;Lingxin Chen","doi":"10.1016/j.aquatox.2025.107257","DOIUrl":"10.1016/j.aquatox.2025.107257","url":null,"abstract":"<div><div>Antibiotics and microplastics (MPs) are two classes of emerging contaminants that are commonly found in various water environments. However, how different sized MPs affect the toxicity and biodegradation of antibiotics remains poorly understood. We investigated the effects of polystyrene (PS) MPs with different particle sizes (100 nm and 30 μm) on the physiological responses and degradation behavior of <em>Phaeodactylum tricornutum</em> to sulfamerazine (SMR). Results showed that microalgae growth was inhibited by SMR, and MPs especially those of smaller size exacerbated the inhibitory effects of SMR on microalgae, including decreasing the content of chlorophyll a, carotenoids, malondiadehyde and superoxide dismutase activity. MPs exhibited low adsorption towards SMR, and MPs especially 30 μm MPs strengthened SMR photodegradation through leaching more organic chemicals. In comparison, 100 nm MPs obstructed the light, resulting in insignificant effects on photodegradation. Apart from photodegradation, SMR could be bioaccumulated and biodegraded by microalgae, and biodegradation was the main removal mechanism. The overall influence of MPs on SMR degradation by microalgae was a balance of the promotion on photodegradation and negative effects on microalgae growth, with the degradation efficiency and rate of SMR significantly lower in treatment of 100 nm MPs (0.0128 ± 0.0012 day⁻¹, 30.13 ± 0.36 %) than treatments without MPs (0.0155 ± 0.0011 day⁻¹, 32.90 ± 3.11 %) or with 30 μm MPs (0.0165 ± 0.0013 day⁻¹, 34.46 ± 2.52 %). Overall, this study reveals the size-specific effects of MPs on the toxicity and degradation behavior of SMR, providing novel insights into the combined effects of SMR and MPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107257"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multigenerational effects of virgin and sampled plastics on the benthic macroinvertebrate Chironomus riparius","authors":"Riccardo Sbarberi ,&nbsp;Stefano Magni ,&nbsp;Benedetta Ponti ,&nbsp;Erica Tediosi ,&nbsp;Maria Chiara Neri ,&nbsp;Andrea Binelli","doi":"10.1016/j.aquatox.2024.107205","DOIUrl":"10.1016/j.aquatox.2024.107205","url":null,"abstract":"<div><div>Although sediments are important reservoirs of plastics, most of the ecotoxicological studies on these contaminants are focused on the organisms living in the water column, while only a smaller number of evidence concerns the plastic impact on benthic species. Therefore, this study compared the multigenerational effects on the sediment-dwelling midge <em>Chironomus riparius</em> exposed to both virgin polystyrene microbeads (22,400–224,000 plastics/kg sediments dry weight), and plastic mixtures (40–420 plastics/kg dry weight) collected from four of the main tributaries of Po River (Ticino, Adda, Oglio and Mincio Rivers, Northern Italy) to evaluate the role played by other characteristics related to these physical contaminants in determining their toxicity as opposed to concentration alone. The modified Chironomid Life-Cycle Toxicity Test (OECD 233) was used to evaluate the multigenerational effects on the Emergence and Development Rates, Fecundity and Fertility. In addition, a biomarkers’ suite of cellular stress, neurotoxicity, and energetic metabolism was applied in the 2^nd generation (2^nd/3^rd instar of larvae) to investigate the potential mechanisms associated to the apical effects. Our results showed no significant (<em>p</em> &gt; 0.05) multigenerational effect for any of the endpoints tested for the virgin plastics’ exposures. Coherently, no significant effects on biomarkers were measured. Concerning the sampled plastics, the particles collected in Adda River instead induced a significant decrease (<em>p</em> &lt; 0.05) of the Emergence Rate in the 2^nd generation, suggesting that this parameter was the most susceptible among those measured. These results highlight that the different plethora of polymers, sizes and shapes of plastics sampled in natural ecosystems, compared to homogeneous characteristics of virgin polystyrene microbeads, appears to have considerable importance over concentration alone in determining the toxicity of these emerging contaminants.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107205"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetrachlorantraniliprole induces neurodevelopmental toxicity through oxidative stress-mediated apoptosis and dysregulation of Wnt signaling pathway","authors":"Ru Guo ,&nbsp;Youjuan Wu ,&nbsp;Tingting Yu ,&nbsp;Chen Guo ,&nbsp;Zijia Yin ,&nbsp;Guannan Zhao ,&nbsp;Meifeng Li ,&nbsp;Huiling Mao ,&nbsp;Chengyu Hu ,&nbsp;Xiaowen Xu","doi":"10.1016/j.aquatox.2024.107229","DOIUrl":"10.1016/j.aquatox.2024.107229","url":null,"abstract":"<div><div>Tetrachlorantraniliprole (TCTP) is a novel bisamide insecticide and widely used to protect against lepidopteran insect species. However, the application of TCTP in rice fields often leads to water pollution, posing threats to aquatic organisms and potentially to human health. Few studies have assessed the toxic effects of TCTP on aquatic animals. In this study, we used zebrafish as a model organism to evaluate the toxicity of TCTP. Our findings indicated that TCTP adversely affected the development of zebrafish larvae, impacting parameters such as heart rate, body length, and pericardial edema. Exposure to TCTP resulted in the increased embryo mortality along with higher concentrations of the compound. The expression of neurodevelopment-related genes was inhibited in embryos exposed to TCTP. Hematoxylin and eosin (HE) staining revealed that TCTP caused damage to the brain cells of the embryos. Behavioral analysis showed a reduction in activity of the larvaes, which aligned with a decrease in acetylcholinesterase (AChE) activity. Additionally, RNA sequencing (RNA-seq) was employed to elucidate the mechanisms of toxicity. GO and KEGG analysis identified that the pathways were related to oxidative stress, apoptosis and Wnt signaling. We observed an increase of reactive oxygen species (ROS) and Ca²/Mg²-ATPase activity, while antioxidant enzyme activities (SOD, MDA, CAT, Na/K-ATPase and T-ATPase) were significantly decreased in TCTP-exposed groups. Furthermore, TCTP induced brain cells apoptosis, as evidenced by the upregulation of pro-apoptotic genes (<em>bax, p53, TNFα, caspase3</em> and <em>caspase9</em>) and the downregulation of anti-apoptotic gene (<em>bcl2</em>). Moreover, TCTP increased the expression of genes involved in Wnt signaling pathway. Notably, oxidative stress and neuronal damage induced by TCTP could be mitigated by astaxanthin, an antioxidant. Additionally, IWR-1, an inhibitor of Wnt signaling pathway, effectively alleviated the upregulation of genes associated with TCTP treatment and inhibited oxidative stress-induced apoptosis. In conclusion, this study demonstrated TCTP-induced defects of neurodevelopment and the brain cells in zebrafish larvae which were primarily driven by oxidative stress-induced apoptosis and dysregulation of Wnt signaling pathway. Importantly, these toxic phenotypes can be rescued by treatment with astaxanthin or IWR-1.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107229"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arsenite-induced liver apoptosis via oxidative stress and the MAPK signaling pathway in marine medaka","authors":"Jiangtian Lin ,&nbsp;Ting Zhang ,&nbsp;Li Zhang","doi":"10.1016/j.aquatox.2024.107226","DOIUrl":"10.1016/j.aquatox.2024.107226","url":null,"abstract":"<div><div>Arsenic (As) is widely recognized for its hazards to aquatic organisms; however, its toxicological impacts on apoptosis in marine fish remain inadequately explored. This study investigated the effects of <em>in vivo</em> dietary exposure to 50 or 500 mg/kg AsIII (as NaAsO₂) over 28 days in marine medaka, alongside <em>in vitro</em> exposure to 50–750 μg/L AsIII for 48 h in a hepatic cell line derived from marine medaka, to elucidate the toxicity and underlying molecular mechanisms. <em>In vivo</em>, As significantly accumulated in liver tissue (1.79-fold compared to the control), causing hepatic lesions and increased apoptosis (4.85 ± 0.56 % and 9.29 ± 1.82 %, respectively). Gene expression analysis showed downregulation of <em>bcl2l1</em> and upregulation of <em>bax, caspase-3</em> and <em>caspase-9</em>, indicating mitochondrial pathway-mediated apoptosis. <em>In vitro</em>, As exposure induced hepatocyte morphological changes, reactive oxygen species (ROS) production, and apoptosis. Additionally, <em>mapk1</em> and <em>mapk3</em> (ERK pathway) were downregulated both <em>in vivo</em> and <em>in vitro</em>, while <em>mapk14a</em> (P38 pathway), <em>mapk8b</em> and <em>mapk9</em> (JNK pathway) were upregulated exclusively in hepatocytes. Furthermore, n-acetyl cysteine (NAC) attenuated As-induced apoptosis and modulated the expression of MAPK signaling pathway genes, including <em>mapk3</em> and <em>mapk8b</em>, suggesting that As-induced oxidative stress regulates apoptosis via the MAPK signaling pathway. In contrast, phenylbutyric acid (PBA) was ineffective in preventing apoptosis. Overall, these results demonstrate that As induces endogenous apoptosis through oxidative stress and the MAPK signaling pathway in marine medaka.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107226"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for low bioavailability of dietary nanoparticulate cerium in a freshwater food chain","authors":"Lisa A. Golding ,&nbsp;Paul Callaghan ,&nbsp;Brad M. Angel ,&nbsp;Graeme E. Batley ,&nbsp;Grant Griffiths ,&nbsp;An Nguyen ,&nbsp;Tom Cresswell","doi":"10.1016/j.aquatox.2024.107228","DOIUrl":"10.1016/j.aquatox.2024.107228","url":null,"abstract":"<div><div>Radioactive ¹⁴¹Ce in ionic (I-Ce), nano (N-Ce, 11 ± 9 nm mean primary particle size ± standard deviation) and micron-sized (M-Ce, 530 ± 440 µm) forms associated with natural and artificial diets in natural river water and synthetic freshwater were used to measure the real-time biokinetics of dietary ¹⁴¹Ce assimilation in a freshwater food chain. The model food chain consisted of microalgae (<em>Raphidocelis subcapitata</em>), snails (<em>Potamopyrgus antipodarum</em>) and prawns (<em>Macrobrachium australiense</em>). Pulse-chase experiments showed that 91–100 % of all forms of cerium associated with all diets and water types were eliminated from the digestive system of the snail and prawn within 24 h, with no detectable cerium assimilation. The prawn and snail median elimination times (ET50) and elimination rates (Ke) for all cerium forms ranged from 0.05 to 1.7 d, and 30 to &gt;100 % per d, respectively. The pulse-chase results were supported by the autoradiographic evidence for N-Ce and M-Ce that confirmed no detectable assimilation and translocation within the tissue of the prawn over time. In contrast, the more soluble I-Ce was found to be associated in low quantities with the hepatopancreas in the prawn confirming that the lack of dissolution by N-Ce and M-Ce in the digestive environment of these organisms makes these forms less bioavailable. In addition, hetero-agglomeration of N-Ce and M-Ce resulted in particles that did not dissociate in digestive fluids and were too large to be assimilated thereby making them non-bioavailable. Based on the results from this study and from the literature review, the risk of N-Ce biomagnification and chronic dietary toxicity in freshwater ecosystems is no greater than the risk associated with M-Ce or I-Ce.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107228"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gender effects of nanoplastics and emerging contaminants mixtures in Mytilus galloprovincialis","authors":"J.M. Gonçalves ,&nbsp;M. Benedetti ,&nbsp;G. d'Errico ,&nbsp;F. Regoli ,&nbsp;M.J. Bebianno","doi":"10.1016/j.aquatox.2024.107219","DOIUrl":"10.1016/j.aquatox.2024.107219","url":null,"abstract":"<div><div>The reproduction of mussels occurs within the water column, and if gametogenesis is successful, gametes are exposed to the surrounding contaminants. Nanoplastics and other emerging contaminants have been gaining vast attention; however, their effects on the reproductive tissues of mussels with sex differentiation are scarce. Here, the effects of polystyrene nanoparticles (50 nm; 10 µg/L), the cytotoxic drug 5-fluorouracil (10 ng/L), and a mixture of the two were evaluated in the gonads of <em>Mytilus galloprovincialis</em> after a 21-day exposure for a multi-biomarker assessment, and after 28 days for the accumulation of nanoplastics. The effects on the activity of superoxide dismutase, catalase, glutathione-<em>S</em>-transferase, and lipid peroxidation were evaluated. Moreover, synergistic and antagonistic interactions in the mixture were calculated. A weight of evidence model was also used to elaborate on the hazardous level of biomarker results relative to polystyrene nanoparticles alone and in the mixture. The accumulation of nanoplastics appeared gender and time-specific, with females mostly compromised. According to the data set, a synergistic interaction between the cytotoxic drug and the nanoplastics makes the combination far more dangerous than individual stressors. The Weight Of Evidence model also confirms that females are more compromised at chronic exposure times than males. This study shows that the uptake, fate, and impact of emerging contaminants of concern can be significantly influenced by sex.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107219"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing predictive models for assessing LC50 of organic contaminants in Gammarus species using interpretable structural parameters","authors":"Mehran Karimi ,&nbsp;Eskandar Kolvari ,&nbsp;Mohammad Hossein Keshavarz ,&nbsp;Nadiya Koukabi","doi":"10.1016/j.aquatox.2025.107235","DOIUrl":"10.1016/j.aquatox.2025.107235","url":null,"abstract":"<div><div><em>Gammarus</em> species are crucial indicators of environmental health, making them important for ecological studies and water quality assessments. They offer a wide range of specific responses regarding the median lethal concentration (<em>LC₅₀</em>) of organic compounds. This research presents four predictive models to determine the <em>LC₅₀</em> of chemicals impacting selected gammarid amphipods: <em>Gammarus lacustris, Gammarus fasciatus, Gammarus pulex</em>, and <em>Gammarus pseudolimnaeus</em>. These species are recognized for their sensitivity to various pollutants and are among the most sensitive aquatic invertebrates. The new models provide straightforward methods for estimating the <em>pLC₅₀</em> (−log <em>LC₅₀</em>/molecular weight) of various organic compounds based on interpretable structural parameters including the number of effective functional groups, the types of atoms present, and various structural characteristics of organic molecules. This study aims to leverage the largest available experimental dataset compared to prior quantitative structure–activity relationship (QSAR) models for these gammarid amphipods. The dataset contained toxicity data for 91 compounds affecting <em>Gammarus fasciatus</em>, 50 for <em>Gammarus lacustris</em>, and 48 each for <em>Gammarus pseudolimnaeus</em> and <em>Gammarus pulex</em>, aligning with comparative QSAR models. External datasets included 13 compounds for <em>Gammarus fasciatus</em>, 2 for <em>Gammarus lacustris</em>, and 6 for <em>Gammarus pseudolimnaeus</em>. Efforts focus on using interpretable structural parameters of organic compounds rather than computer-based descriptors, as outlined in the existing QSAR models. For the species G<em>. fasciatus</em>, G<em>. lacustris</em>, G<em>. pseudolimnaeus</em>, and G<em>. pulex</em>, the R² ratios for the new models versus the best QSAR models are 0.915/0.728, 0.955/0.747, 0.976/0.769, and 0.970/0.768, respectively. The higher R² values in the new models demonstrate greater reliability and robustness in capturing the data's underlying relationships.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107235"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tributyl phosphate inhibits neurogenesis and motor functions during embryonic development in zebrafish","authors":"Gourav Chakraborty ,&nbsp;Bhagyashri Joshi ,&nbsp;Kedar Ahire ,&nbsp;Chinmoy Patra","doi":"10.1016/j.aquatox.2024.107203","DOIUrl":"10.1016/j.aquatox.2024.107203","url":null,"abstract":"<div><div>Tributyl phosphate (TBP), an organophosphate ester (OPE), is heavily used as a solvent in chemical industries, a plasticizer, and to extract radioactive molecules. Thus, widespread uses of TBP in industrialized countries led to the release of TBP and its metabolites, dibutyl phosphate (DBP) and monobutyl phosphate (MBP), in the environment and were detected in human samples. Accumulating these OPEs over time in humans and aquatic animals may develop toxicological effects. The reports also say TBP passes through the mother-fetal transmission route and may affect embryonic development. However, the impact of TBP and its metabolites on vertebrate development has been poorly studied. <em>Ex-utero</em> development, high fecundity, and optical transparency make the zebrafish a preferred model for toxicological evaluation. Thus, we aim to explore the toxic effects of TBP and its metabolites on aquatic animals using zebrafish as a model organism. Embryos in the chorion were incubated in 10–60 µM test chemicals from 6 to 48 h post fertilization (hpf), and analyzed the adverse effects on embryos. Our study found that 10–20 µM TBP inhibits neural growth, resulting in decreased spontaneous movement frequency and locomotive behavior without altering the overall embryonic growth and muscle functions. In contrast, DBP-treated embryos showed increased spontaneous movement frequency without changing the motor neuron growth and locomotive behavior. Further, in higher concentrations, TBP is teratogenic, and DBP is lethal to the embryos. Altogether, we found that TBP inhibits neurogenesis and motor behavior; however, its metabolite DBP is neuroexcitatory in zebrafish embryos.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107203"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}