Aquatic Toxicology最新文献_第9页

Marine ecotoxicity evaluation of 10 per- and poly-fluoroalkyl acids using three USEPA short-term chronic bioassays 使用三种USEPA短期慢性生物测定法评价10种单氟和多氟烷基酸的海洋生态毒性

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-10-28 DOI: 10.1016/j.aquatox.2025.107625

Nicholas T. Hayman , Zacharias Pandelides , Josiah Discar , Marienne A. Colvin , Gunther Rosen , Jennifer Arblaster , Jason Conder

{"title":"Marine ecotoxicity evaluation of 10 per- and poly-fluoroalkyl acids using three USEPA short-term chronic bioassays","authors":"Nicholas T. Hayman , Zacharias Pandelides , Josiah Discar , Marienne A. Colvin , Gunther Rosen , Jennifer Arblaster , Jason Conder","doi":"10.1016/j.aquatox.2025.107625","DOIUrl":"10.1016/j.aquatox.2025.107625","url":null,"abstract":"<div><div>Effects data for per- and poly-fluoroalkyl substances (PFAS) for marine aquatic life are lacking, limiting the assessment of site-specific ecological risks in marine ecosystems and development of marine water quality criteria. In this study, the toxicity of 10 PFAS (PFBA, PFHxA, PFOA, PFDA, PFBS, PFHxS, PFOS, PFDS, 6:2 FTS, and 8:2 FTS) were evaluated with three standard marine toxicity testing species: Mediterranean mussel (<em>Mytilus galloprovincialis</em>); purple sea urchin (<em>Strongylocentrotus purpuratus</em>); and giant kelp (<em>Macrocystis pyrifera</em>). Many tests failed to elicit responses exceeding 50 % adverse effect levels, despite approaching solubility limits. EC<sub>50</sub> values were able to be derived in only 43 % of the toxicity tests, and were generally above 1 mg/L, except for PFOS and PFDA based on the most sensitive of the three species (<em>S. purpuratus</em> and <em>M. galloprovincialis</em>), with EC<sub>50</sub>s in the approximate 0.1 to 1 mg/L range. <em>M. pyrifera,</em> the macroalgae, was less sensitive than either invertebrate species. EC<sub>50</sub> values for all three species decreased with perfluoroalkyl carbon chain length, as increasing toxicity was observed in longer chained compounds, and a statistically significant relationship between chain length and EC<sub>50</sub> was detected for <em>M. galloprovincialis</em>. A number of other toxicological metrics (NOECs, LOECs, EC<sub>10</sub>s, and EC<sub>20</sub>s) were also generated. NOECs for sublethal endpoints were in the 0.01 to 100 mg/L range and were orders of magnitude higher than environmentally relevant concentrations. Based on the results in this study, adverse effects on these species and endpoints would not be expected to occur in the PFAS-impacted marine environment.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107625"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145383278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of pyrethroid insecticides on sex ratios in agile frogs (Rana dalmatina) 拟除虫菊酯类杀虫剂对敏捷蛙性比的影响

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-10-28 DOI: 10.1016/j.aquatox.2025.107624

Emese Balogh , Szabolcs Hócza , Nikolett Ujhegyi , Andrea Kásler , Dóra Holly , Dávid Herczeg , János Ujszegi , Zoltán Gál , Orsolya I. Hoffmann , Veronika Bókony , Zsanett Mikó

{"title":"Effects of pyrethroid insecticides on sex ratios in agile frogs (Rana dalmatina)","authors":"Emese Balogh , Szabolcs Hócza , Nikolett Ujhegyi , Andrea Kásler , Dóra Holly , Dávid Herczeg , János Ujszegi , Zoltán Gál , Orsolya I. Hoffmann , Veronika Bókony , Zsanett Mikó","doi":"10.1016/j.aquatox.2025.107624","DOIUrl":"10.1016/j.aquatox.2025.107624","url":null,"abstract":"<div><div>Environmental pollutants have the potential to alter sex ratios in wildlife through sex-biased mortality. Furthermore, endocrine disruptors may cause sex reversal during early ontogeny in ectothermic vertebrates, resulting in a phenotypic sex that is not concordant with the genotypic sex encoded by the sex chromosomes. Despite the wide-ranging implications of these sex-ratio biasing effects, they are rarely studied in ecotoxicology, especially in a way that allows for disentangling the two mechanisms. We investigated these effects of two synthetic pyrethroids, deltamethrin and etofenprox, that are commonly used insecticides and have been linked to adverse effects on fish and amphibian biodiversity. We assessed the effects of environmentally relevant concentrations of these two pyrethroids on phenotypic sex ratio, sex-dependent mortality, and sex reversal in agile frogs (<em>Rana dalmatina</em>). Tadpoles from field-collected eggs were reared in mesocosms until metamorphosis by adding 0.03 or 0.3 μg/L of deltamethrin or etofenprox three times to the water. We observed no effect in three of the four treatment groups. However, in the lower-concentration etofenprox treatment, phenotypic sex ratio was male-biased two months post-metamorphosis, and genotypic sexing revealed that this was due to female-biased mortality during metamorphosis and not to sex reversal. Although the estimation certainty of these effects was somewhat limited, they highlight that not all sex-ratio distorting effects are caused by sex reversal. Therefore, ecotoxicological studies aiming to understand the endocrine distruptor effects of environmental contaminants should strive to separate the effects on sex determination and sex-dependent mortality.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107624"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145383282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined impacts of a lipid lowering drug, gemfibrozil, and temperature on bioenergetics and digestive gland function of a marine bivalve Mytilus edulis 降脂药、吉非罗齐和温度对海洋双壳贝贻贝生物能量学和消化腺功能的综合影响

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-11-21 DOI: 10.1016/j.aquatox.2025.107654

Inna M. Sokolova , Halina Falfushynska , Eugene P. Sokolov , Esther Blanco-Rayón , Xabier Lekube , Ionan Marigómez

{"title":"Combined impacts of a lipid lowering drug, gemfibrozil, and temperature on bioenergetics and digestive gland function of a marine bivalve Mytilus edulis","authors":"Inna M. Sokolova , Halina Falfushynska , Eugene P. Sokolov , Esther Blanco-Rayón , Xabier Lekube , Ionan Marigómez","doi":"10.1016/j.aquatox.2025.107654","DOIUrl":"10.1016/j.aquatox.2025.107654","url":null,"abstract":"<div><div>Pharmaceutical fibrates are increasingly detected in coastal waters, yet their organism-level effects under realistic thermal regimes remain poorly resolved. We tested how environmentally relevant gemfibrozil (GFB) exposure interacts with temperature to alter bioenergetics and digestive gland condition in the blue mussel <em>Mytilus edulis</em> spp. Adult mussels were exposed for 21 days to 15 °C or 20 °C with or without GFB (25 µg L⁻¹). We quantified clearance rate, assimilation efficiency, energy ingestion, oxygen consumption, ammonium excretion, and scope for growth (SFG), and assessed lysosomal membrane stability and structure, cathepsin D activity, intracellular neutral lipids, and histopathology of the digestive gland. Warming to 20 °C increased clearance rate (∼2.8-fold) and energy ingestion but reduced assimilation efficiency (from 93% to 57%); SFG was consequently higher at 20 °C. GFB and temperature interacted on respiration: at 15 °C GFB depressed oxygen consumption, whereas at 20 °C GFB elevated it; ammonium excretion was unchanged. GFB destabilized lysosomal membranes and led to an increase in the lysosomal volume and surface area, particularly at 20 °C. Histologically, GFB induced epithelial thinning and digestive gland atrophy, accompanied by loss of diverticula integrity and depletion of adipogranular cells at 20 °C. Overall, GFB acts as a metabolic disruptor in the mussels, impairing digestive gland health. Moderate warming partly offsets energetic costs by enhancing filtration and energy acquisition but does not reverse cellular and tissue-level damage in the digestive gland. These results indicate that warming coastal seas may buffer some functional consequences of fibrate exposure while allowing persistent sub-lethal pathology, with implications for long-term ecological risk to marine ecosystems from lipid-lowering drugs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107654"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145567432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image-based morphological characterization of the erythrocytic shape of green turtle (Chelonia mydas) for its potential use as an environmental biomarker 基于图像的绿龟红细胞形态特征及其作为环境生物标志物的潜在应用。

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-11-12 DOI: 10.1016/j.aquatox.2025.107645

Claudia Lorena Rodríguez-Salazar , Mauricio Comas-García , Javier Flavio Vigueras-Gómez , Claudia G. Castillo , Adriana Monsiváis-Urenda , Tania Zenteno-Savín , Vanessa Labrada-Martagón

{"title":"Image-based morphological characterization of the erythrocytic shape of green turtle (Chelonia mydas) for its potential use as an environmental biomarker","authors":"Claudia Lorena Rodríguez-Salazar , Mauricio Comas-García , Javier Flavio Vigueras-Gómez , Claudia G. Castillo , Adriana Monsiváis-Urenda , Tania Zenteno-Savín , Vanessa Labrada-Martagón","doi":"10.1016/j.aquatox.2025.107645","DOIUrl":"10.1016/j.aquatox.2025.107645","url":null,"abstract":"<div><div>Environmental pollutants increase genotoxic damage in green sea turtles (<em>Chelonia mydas</em>). Erythrocytic nuclear abnormalities (ENA) have been identified using the micronucleus (MN) test, a biomarker of DNA damage. The MN test is time-consuming, and counting depends on the observer, who requires training to avoid false positives. This study aimed to evaluate the utility of image-based morphometric measurements of erythrocyte nuclear shape to assess differences among green turtles from the Mexican Caribbean inhabiting four feeding sites with varying levels of anthropogenic pressure. An semi-automated measurement of 350 nuclei per animal (<em>n</em> = 60) per study site (<em>n</em> = 15) was performed, optimizing the traditional, time-consuming process of 1000–2000 cell counts. Eight metrics of the nuclei morphology were obtained and correlated with the frequency of ENA. A non-linear regression analysis was performed at each study site to infer the focus distance (FD) term from the aspect ratio (AR) and to evaluate deviations from the ellipse in cases with nuclear abnormalities. The erythrocyte nuclei’s minor axis, AR, FD, circularity, and roundness were correlated with the number of ENA. The green turtles captured in the most highly anthropized sites (<em>i.e.,</em> Akumal and Xcalak) showed the highest frequency of ENA, the smallest area, and the nuclear form most deviated from the elliptical nuclear erythrocytic shape expected. The proposed methodology enabled differentiation of sea turtles’ habitat conditions. Results from this study agree with previous reports of the highest concentrations of organic persistent pollutants, genotoxic damage, and alterations in oxidative stress in green turtles inhabiting urbanized areas of the Mexican Caribbean.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107645"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145525053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic toxicity of PAHs in Ruditapes philippinarum: Metabolic pathways and hepatotoxicity 菲律宾蛤中多环芳烃的代谢毒性：代谢途径和肝毒性

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-11-12 DOI: 10.1016/j.aquatox.2025.107643

Qiuhong Xu, Qiaoqiao Wang, Ruyue Ma, Rongjun Cai, Songhui Xie, Jingjing Miao, Luqing Pan

{"title":"Metabolic toxicity of PAHs in Ruditapes philippinarum: Metabolic pathways and hepatotoxicity","authors":"Qiuhong Xu, Qiaoqiao Wang, Ruyue Ma, Rongjun Cai, Songhui Xie, Jingjing Miao, Luqing Pan","doi":"10.1016/j.aquatox.2025.107643","DOIUrl":"10.1016/j.aquatox.2025.107643","url":null,"abstract":"<div><div>Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants that exhibit high bioaccumulation and toxicity, posing a significant threat to marine ecosystems. This study integrated transcriptomics, computational modeling, and experimental validation to elucidate the detoxification metabolism and hepatotoxicity of a PAHs mixture (phenanthrene: chrysene: benzo[a]pyrene = 1:1:1) in the Manila clam (<em>Ruditapes philippinarum</em>). PAHs exposure activated detoxification genes via aryl hydrocarbon receptor and HR96-like pathways, with additional modulation through neurotransmitter-mediated NF-κB and tyrosine kinase signaling. Phase I (e.g., CYP1A1, CYP3A4) and Phase II enzymes were upregulated, yet persistent reactive oxygen species accumulation caused macromolecular damage. Females exhibited lower detoxification but greater oxidative stress than males. Chronic oxidative injury induced liver impairment, probably accompanied by inflammation and fibrosis. These findings reveal sex-dependent toxicological responses and provide mechanistic insights for ecological risk assessment and biomarker development in marine pollution monitoring.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107643"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145515556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The combined pollution of imidacloprid and glufosinate-ammonium aggravates toxicity on Hydrilla verticillata and epiphytic microbial community 吡虫啉和草铵膦复合污染加重了对水螅和附生微生物群落的毒性

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-11-16 DOI: 10.1016/j.aquatox.2025.107648

Xiaodan Zhang , Songhe Zhang , Hongyu Guo , Wenjie Yuan , Xin Lv , Tilang Zhang , Shuaijie Jiang

{"title":"The combined pollution of imidacloprid and glufosinate-ammonium aggravates toxicity on Hydrilla verticillata and epiphytic microbial community","authors":"Xiaodan Zhang , Songhe Zhang , Hongyu Guo , Wenjie Yuan , Xin Lv , Tilang Zhang , Shuaijie Jiang","doi":"10.1016/j.aquatox.2025.107648","DOIUrl":"10.1016/j.aquatox.2025.107648","url":null,"abstract":"<div><div>The combined effects of imidacloprid (ICD) and glufosinate-ammonium (GLAM) on submerged plants and epiphytic microbial community remains unclear, despite the frequent co-occurrence of these pesticides in aquatic environments. In this study, a 24-day experiment was conducted to examine changes in water quality, plant physiology, and epiphytic microbial communities in <em>Hydrilla verticillate</em>-dominated wetlands under ICD alone or together with GLAM. Results showed that GLAM+ICD induced greater oxidative stress and a stronger antioxidant response in <em>H. verticillata</em> compared ICD alone, demonstrating that the mixtures exacerbated ecotoxicological effects on plant. Compared to ICD, many micro-biomarkers were obtained in ICD+GLAM, including Bacteroidota, Comamonadaceae, Proteobacteria and <em>Cocconeis</em>. Redundancy analysis indicated that the ICD concentration was correlated positively to <em>Stigeoclonium, Closterium</em> and <em>Cocconeis</em>, but negatively related with <em>Oedogonium</em> and <em>Chloromonas</em>, GLAM is opposite to ICD. More complex interkingdom interactions among bacteria and eukaryotes were observed in high concentration MIX treatment group compared to others. Co-occurrence networks analysis further demonstrated that GLAM and ICD changed microbial interactions within epiphytic biofilm, and impaired the stability and function of the microbial communities<strong>.</strong> These results underscore that the combined presence of ICD and GLAM poses a significant ecological risk to <em>Hydrilla verticillate-</em>biofilm system in agricultural regions.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107648"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145536193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endocrine disruptive effects and mechanisms of isothiazolinone analogs in marine medaka (Oryzias melastigma) embryos and H295R cells 异噻唑啉酮类似物对海洋medaka胚胎和H295R细胞的内分泌干扰作用及其机制

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-11-01 DOI: 10.1016/j.aquatox.2025.107633

Jiali Li , Lizhu Tang , Jing Li , Chenyan Hu , Jiahui Cao , Wenbing Zhang , Lianguo Chen

{"title":"Endocrine disruptive effects and mechanisms of isothiazolinone analogs in marine medaka (Oryzias melastigma) embryos and H295R cells","authors":"Jiali Li , Lizhu Tang , Jing Li , Chenyan Hu , Jiahui Cao , Wenbing Zhang , Lianguo Chen","doi":"10.1016/j.aquatox.2025.107633","DOIUrl":"10.1016/j.aquatox.2025.107633","url":null,"abstract":"<div><div>The application of isothiazolinones as antimicrobial agents has caused prevalent pollution of marine environment. However, the developmental toxicity and endocrine disrupting effects of different isothiazolinone analogs to marine organisms remain largely elusive. In the present study, marine medaka embryos were exposed to 0, 1, 3, and 10 μg/L of six representative isothiazolinone analogs, including isothiazole, methylisothiazolinone (MIT), methylchloroisothiazolinone (MCI), octylisothiazolinone (OIT), benzisothiazolinone (BIT), and dichlorocthylisothiazolinone (DCOIT), after which the anomalies in early embryogenesis and endocrine homeostasis were examined. <em>In vivo</em> results showed that OIT, BIT, and DCOIT were potently estrogenic, which was characterized by the higher estradiol (E<sub>2</sub>) content and remarkable elevation of E<sub>2</sub>/testosterone ratio in larval medaka. Correlation analysis found significantly positively relationship among OIT, BIT, and DCOIT, indicating their similar modes of toxicity. Besides, <em>in vitro</em> exposure using H295R cells was performed to gain more insights into endocrine disrupting mechanisms, further confirming the multiple disturbances of DCOIT in reproductive endocrine cascades, consisting of MAPK proteins, aromatase, and sex hormones. Overall, the present findings underscore the high susceptibility of marine organisms to the developmental and endocrine toxicity of isothiazolinones, especially during early embryonic stages. In the future, the marine ecological risks of isothiazolinones need to be evaluated urgently, thus facilitating chemical lifecycle management.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107633"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145423999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular responses to pollution stress in glass eels (Anguilla anguilla): Gene expression changes associated with varying contamination levels and temperature across estuaries 玻璃鳗（Anguilla Anguilla）对污染应激的分子反应：与河口污染水平和温度变化相关的基因表达变化

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-10-28 DOI: 10.1016/j.aquatox.2025.107623

Alvaro Gutierrez-Rodriguez , Claudia Nuñez-Moyano , Lucía Rivas-Iglesias , Susana Acle , Luis Jose Royo-Martin , Eva Garcia-Vazquez , Gonzalo Machado-Schiaffino

{"title":"Molecular responses to pollution stress in glass eels (Anguilla anguilla): Gene expression changes associated with varying contamination levels and temperature across estuaries","authors":"Alvaro Gutierrez-Rodriguez , Claudia Nuñez-Moyano , Lucía Rivas-Iglesias , Susana Acle , Luis Jose Royo-Martin , Eva Garcia-Vazquez , Gonzalo Machado-Schiaffino","doi":"10.1016/j.aquatox.2025.107623","DOIUrl":"10.1016/j.aquatox.2025.107623","url":null,"abstract":"<div><div>Understanding the effects of global change is crucial to aid in the conservation of critically endangered species such as the European eel (<em>Anguilla anguilla</em>). Emerging pollutants (microplastics) and legacy ones such as heavy metals, as well as thermal pollution, are deleterious for this species; however, their molecular impacts on glass eels entering estuaries remain understudied. Investigating the molecular signatures of environmental stress can provide insights into some of the factors contributing to <em>A. anguilla</em> population decline. In this study, expression changes in candidate genes associated with general and thermal stress (<em>hsp70</em> & <em>hsp90</em>), oxidative stress (<em>sod1, sod2, cat, gpx1</em>), heavy metal detoxification (<em>mt2</em>), and neurotoxicity (<em>ache</em>) were measured in gill tissue of glass eels exposed to polystyrene (PS) microbeads in an aquarium, and also in individuals sampled from six Bay of Biscay estuaries with different levels of microplastics, heavy metals, nitrites, nitrates and thermal pollution. In the aquarium experiment, a significant positive correlation was found between <em>hsp70</em> expression and PS concentration. On the other hand, in the glass eels sampled from the wild results showed that gene expression patterns tend to cluster based on estuary’s pollution levels. Specifically, <em>hsp70</em> gene expression was upregulated in eels from the most polluted estuaries, suggesting signs of damage. This study highlights the molecular effects and signatures of emerging and legacy pollutants on the critically endangered European eel, under natural and controlled conditions.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107623"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145383280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling effects of resorcinol: Morphological alterations in the retina and thyroid follicles of zebrafish (Danio rerio) embryos 间苯二酚的解旋效应：斑马鱼胚胎视网膜和甲状腺滤泡的形态学改变

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-11-10 DOI: 10.1016/j.aquatox.2025.107642

Imen Ben Chabchoubi , Marian Stoll , Maximilian Rinderknecht , Luisa Reger , Laura Behnstedt , Thomas Braunbeck , Lisa Baumann , Olfa Hentati , Lisa Gölz

{"title":"Unravelling effects of resorcinol: Morphological alterations in the retina and thyroid follicles of zebrafish (Danio rerio) embryos","authors":"Imen Ben Chabchoubi , Marian Stoll , Maximilian Rinderknecht , Luisa Reger , Laura Behnstedt , Thomas Braunbeck , Lisa Baumann , Olfa Hentati , Lisa Gölz","doi":"10.1016/j.aquatox.2025.107642","DOIUrl":"10.1016/j.aquatox.2025.107642","url":null,"abstract":"<div><div>The hypothalamus-pituitary-thyroid (HPT) axis is a central endocrine regulatory network crucial for morphological development and physiological homeostasis in vertebrates. It is particularly sensitive to disruption by Endocrine Disrupting Chemicals (EDCs), which may lead to adverse effects at the individual and population levels. Resorcinol, an environmentally relevant compound used extensively in industry and cosmetics, has been identified as a potential disruptor of the thyroid hormone system (THS). However, data on its effects in aquatic organisms, especially fish, remain scarce. Recent studies and adverse outcome pathways (AOPs) even suggest a link between thyroid hormone system (THS) disruption and retinal layer alterations in fish. In this study<em>,</em> transgenic <em>Tg(tg:MA-mCherry)</em> zebrafish (Danio rerio) embryos were exposed to resorcinol for 120 h to assess effects on thyroid follicle morphology, retinal development and behaviour. Thyroid follicles were analysed using in vivo fluorescence microscopy. Retinal layers were evaluated histopathologically and behaviour changes were assessed using the light–dark locomotion test. Exposure to 1 and 30 mg/L resorcinol resulted in thyroid follicle proliferation, likely indicating a compensatory response to inhibited thyroid hormone synthesis. At 100 mg/L, reduced eye size and thinning of multiple retinal layers were observed, suggesting secondary developmental effects. Additionally, increased locomotor activity was detected in exposed embryos. These findings support the classification of resorcinol as a THS-disrupting compound and emphasize the need to incorporate histopathological and behaviour endpoints into existing OECD test guidelines to improve the detection of THS disruptors in fish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107642"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145478142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic and physiological insights into Diethylstilbestrol toxicity in Phaeodactylum tricornutum 三角褐指藻（Phaeodactylum tricornutum）中己烯雌酚毒性的转录组学和生理学研究

IF 4.3 2区环境科学与生态学

Aquatic Toxicology Pub Date : 2026-01-01 Epub Date: 2025-11-03 DOI: 10.1016/j.aquatox.2025.107636

Dong-Sheng Zhao , Xiao-Li Liu , Yu-Ting Chen , Huan Yang , Muhammad Ahsan Farooq , Xiufeng Yan , Hui-Xi Zou

{"title":"Transcriptomic and physiological insights into Diethylstilbestrol toxicity in Phaeodactylum tricornutum","authors":"Dong-Sheng Zhao , Xiao-Li Liu , Yu-Ting Chen , Huan Yang , Muhammad Ahsan Farooq , Xiufeng Yan , Hui-Xi Zou","doi":"10.1016/j.aquatox.2025.107636","DOIUrl":"10.1016/j.aquatox.2025.107636","url":null,"abstract":"<div><div>Diethylstilbestrol (DES), an emerging pollutant, poses a threat to marine ecosystems, but its toxic effects on marine phytoplankton and the underlying mechanisms are not well understood. To address this knowledge gap, this study systematically investigated the physiological and molecular responses of the marine diatom <em>Phaeodactylum tricornutum</em> to DES exposure, aiming to uncover its toxicological mechanisms. DES significantly reduced chlorophyll content and photosynthetic efficiency, impaired the PSII core, and decreased both photosynthesis and respiration rates. The diatom responded by enhancing antioxidant defenses, including superoxide dismutase and catalase activities. DES was also partially removed from the water column, with removal rates of 9–18 %. Transcriptomic analysis revealed extensive changes in genes involved in porphyrin metabolism and carbon fixation, which were closely linked to the observed physiological impairments. In summary, these findings reveal a core toxicological mechanism whereby DES disrupts photosynthesis through inhibition of carbon fixation and porphyrin metabolism, providing new insights into how emerging pollutants impair marine primary producers and offering a scientific basis for the ecological risk assessment of DES contamination.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"290 ","pages":"Article 107636"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145434249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0