Aquatic Toxicology最新文献

{"title":"Polystyrene microplastics exacerbate acetochlor-induced reproductive toxicity and transgenerational effects in zebrafish","authors":"Yang Wang ,&nbsp;Chaofan Ma ,&nbsp;Zenglong Chen ,&nbsp;Yinping Guo ,&nbsp;Yuan Jing ,&nbsp;Xiaolei Chen ,&nbsp;Hongwu Liang","doi":"10.1016/j.aquatox.2024.107208","DOIUrl":"10.1016/j.aquatox.2024.107208","url":null,"abstract":"<div><div>Microplastic (MPs) can adsorb co-existing pollutants, and alter their behavior and toxicity. Meanwhile, amide herbicides like acetochlor (ACT) are widely used in agriculture, with potential endocrine-disrupting effects that raise ecological concerns. The aim of this research was to examine the effects of MPs on the reproductive endocrine disruption caused by ACT and the effects of maternal transmission. Zebrafish were employed in this study to assess the reproductive toxicity of ACT alone and in combination with polystyrene microplastics (PS-MPs) of different sizes (200 nm and 2 μm) and concentrations (0.1 and 1 mg/L) over a 63-day exposure experiment. The results indicated that ACT was concentrated in zebrafish tissues in the order: intestine &gt; liver &gt; gill &gt; brain &gt; gonad &gt; muscle. PS-MPs increased ACT bioaccumulation, worsened gonadal damage, led to abnormalities in hormone levels, and caused disruptions in HPG axis gene expression, further exacerbating the reproductive toxicity. Maternal transfer of ACT affected offspring growth, thyroid function, and HPT axis gene expression, with nanoplastics (NPS) amplifying these adverse effects. This study offers crucial insights into the ecological hazards posed by ACT and PS-MPs, emphasizing the increased toxicity due to PS-MPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107208"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cetacean species found stranded along Croatian coast of the Adriatic Sea as bioindicators of non-essential trace elements in the environment","authors":"Marija Sedak ,&nbsp;Maja Đokić ,&nbsp;Nina Bilandžić ,&nbsp;Tomislav Gomerčić ,&nbsp;Miroslav Benić ,&nbsp;Manuela Zadravec ,&nbsp;Martina Đuras","doi":"10.1016/j.aquatox.2024.107206","DOIUrl":"10.1016/j.aquatox.2024.107206","url":null,"abstract":"<div><div>In tissues of toothed whales from the Adriatic Sea (muscle, liver, kidney, lung, spleen, adipose tissue and skin) the concentrations of cadmium (Cd), lead (Pb) and arsenic (As) were analysed. In total, 186 dolphins were analysed; 155 bottlenose (<em>Tursiops truncatus</em>), 25 striped <em>(Stenella coeruleoalba</em>) and 6 Risso's dolphins (<em>Grampus griseus)</em>. Cadmium concentrations in tissue samples ranged from 0.001 mg/kg in muscle to 16.8 mg/kg wet weight in kidney. Arsenic concentrations in dolphin samples ranged from 0.010 to 12.9 mg/kg ww. The lowest As concentration was found in spleen and highest in liver of bottlenose dolphin. Cadmium and As levels in Risso's dolphins showed higher concentrations in all tissues in comparison to bottlenose and striped dolphins. &gt;50 % of the measured Pb values for all three species of dolphins and examined tissues were lower than 0.1 mg/kg. The accumulation of Cd and As during the lifetime was confirmed. None of the dolphins analysed in this study were exposed to concentrations of Cd in the liver higher than 20 mg/kg wet weight, which can cause renal failure in marine mammals. Numerous species of marine mammals inhabit coastal environments alongside humans and utilize similar food sources, such as fish and cephalopods. Consequently, these mammals can function as valuable indicators of public health concerns.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107206"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary chlorogenic acid supplementation protects against lipopolysaccharide-induced oxidative stress, inflammation and apoptosis in intestine of amur ide (Leuciscus waleckii)","authors":"Yintao Wang ,&nbsp;Sitong Meng ,&nbsp;Denglai Li ,&nbsp;Siying Liu ,&nbsp;Liang LI ,&nbsp;Lifang Wu","doi":"10.1016/j.aquatox.2024.107223","DOIUrl":"10.1016/j.aquatox.2024.107223","url":null,"abstract":"<div><div>In this study, the alleviative effects of chlorogenic acid (CGA) on oxidative stress, inflammation and apoptosis of amur ide (<em>Leuciscus waleckii</em>) induced by lipopolysaccharide (LPS) were evaluated. Using a 2 × 2 factorial design, amur ide were irregularity divided into 4 groups and fed two diets with 0.00 % (CK and LPS), 0.04 % CGA(CGA and LC). After 4-week feeding trial, LPS challenge was executed. Results showed that 0.04 % CGA alleviated LPS-induced intestinal barrier dysfunction by decreasing the levels of 5-HT, D-LA, ET-1 and DAO in serum, increasing <em>ZO-1, Occludin-α, Claudin-c, Claudin-f</em> mRNA, and ZO-1, Occludin, Claudin-1 protein expression, improving intestinal morphology. Moreover, 0.04 % CGA alleviated LPS-induced inflammation and apoptosis by up-regulating <em>TGF-β</em> and <em>Bcl-2</em> mRNA, down-regulating <em>NF-κBp65, TNF-α, Bax, Caspase-3, Caspase-9</em> mRNA and NF-κBp65, Bax, Caspase-3 protein expression. 0.04 %CGA reversed LPS-induced the reduction of GSH-PX, CAT, T-SOD and T-AOC in intestines, whereas MDA showed the opposite result. 0.04 % alleviated LPS-induced the decrease of <em>Nrf2, HO-1, CAT, SOD</em> mRNA and <em>Nrf2</em> protein expression, the increase of <em>Keap1</em> mRNA. Summary, this study suggested that 0.04 % of dietary CGA alleviated LPS-induced intestinal oxidative stress, inflammation and apoptosis of amur ide.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107223"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of the photosynthetic physiology of Ulva lactuca to Cu toxicity under ocean acidification","authors":"Tianpeng Xu ,&nbsp;Zhouyue Lu ,&nbsp;Cheng Chen ,&nbsp;Yuxin Xie ,&nbsp;Jing Ma ,&nbsp;Juntian Xu","doi":"10.1016/j.aquatox.2024.107222","DOIUrl":"10.1016/j.aquatox.2024.107222","url":null,"abstract":"<div><div>Ocean acidification can significantly affect the physiological performance of macroalgae. While copper (Cu) is an essential element for macroalgae and has been extensively studied, the interactive effects of ocean acidification and Cu on these organisms remain less understood. In this study, we measured the photosynthetic characteristics of <em>Ulva lactuca</em> exposed to varying Cu concentrations at two CO₂ levels (415 ppmv, low concentration; 1000 ppmv, high concentration). The results indicated that during chronic toxicity testing, the growth of juvenile <em>U. lactuca</em> significantly increased at Cu concentrations of 0.001 μM, 0.01 μM, and 0.1 μM regardless of low CO₂ concentrations or high CO₂ concentrations condition. In acute toxicity tests, elevated Cu concentrations negatively impacted the growth rate, yield, and photosynthetic rate of <em>U. lactuca</em> under low CO₂ concentrations. Conversely, high CO₂ concentrations enhanced the photosynthetic capacity of <em>U. lactuca</em> with increased Cu concentrations, while the growth rate significantly decreased at Cu concentration of 1.5 μM. Additionally, the activities of peroxidase (POD) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) increased, with an enhancement of malondialdehyde (MDA) content at 1.5 μM Cu under high CO₂ conditions. However, the structure of the chloroplast thylakoid was disrupted by elevated Cu concentrations. These findings suggest that low Cu concentrations promote the growth of <em>U. lactuca</em>, whereas high Cu concentrations inhibit algal growth, and ocean acidification may exacerbate the adverse effects of Cu on <em>U. lactuca</em> in acute toxicity tests.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107222"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of a pharmaceutical pollutant, gemfibrozil, and abiotic stressors (warming and air exposure) on cellular stress responses of the blue mussels Mytilus edulis","authors":"Halina Falfushynska ,&nbsp;Eugene P. Sokolov ,&nbsp;Inna M. Sokolova","doi":"10.1016/j.aquatox.2024.107233","DOIUrl":"10.1016/j.aquatox.2024.107233","url":null,"abstract":"<div><div>Lipid-lowering drugs such as gemfibrozil (GFB) are widely used and highly biologically active, contributing to their persistence in wastewater and subsequent release into aquatic ecosystems. However, the potential impacts and toxic mechanisms of these emerging pollutants on non-target marine organisms, particularly keystone bivalves like <em>Mytilus edulis</em>, remain poorly understood. To address this knowledge gap, we investigated the effects of environmentally relevant concentrations of GFB (25 µg <em>l</em>⁻¹) on oxidative, nitrosative, and dicarbonyl stress in M<em>. edulis</em>, and explored how abiotic stressors such as elevated temperature and air exposure modulate these effects. Our results indicated that GFB and temperature interact to significantly influence oxidative stress markers, including lipid peroxidation (LPO) and protein carbonylation (PC) levels in mussels. Notably, the combination of GFB and warming exhibited antagonistic effects, leading to reduced LPO levels in both submerged and air-exposed mussels. Air exposure alone elevated PC levels across all groups, while warming reduced these levels. Total antioxidant capacity increased during air exposure, with GFB exerting minimal influence on this parameter. Nitrosative stress, as indicated by nitric oxide levels, was significantly affected by GFB only under air exposure conditions. The glutathione system underwent notable alterations, with glutathione reductase activity stimulated during immersion and suppressed during air exposure. Dicarbonyl stress markers, including methylglyoxal and glyoxalase enzyme activities, generally intensified in response to GFB during air exposure. Overall, environmentally relevant concentrations of GFB induced oxidative and dicarbonyl stress in M<em>. edulis</em>, suggesting a shift toward glycolytic metabolism that could impair energy-dependent processes like reproduction. Combined stressor scenarios involving GFB and warming typically exhibited antagonistic rather than synergistic effects. Despite these biochemical disruptions, the mussels demonstrated resilience, particularly during air exposure, highlighting the complexity of environmental stress interactions. These findings emphasize the importance of considering multiple stressors in pollution risk assessments for aquatic ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107233"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omics insights in responses of bivalves exposed to plastic pollution","authors":"Robert Mkuye ,&nbsp;Chuangye Yang ,&nbsp;Fortunatus Masanja ,&nbsp;Salifu Ibrahim ,&nbsp;Xiuyan Yang ,&nbsp;Happiness Mwemi ,&nbsp;Peter Mrope ,&nbsp;Muhammed Salman ,&nbsp;Alma Alfatat ,&nbsp;Yuewen Deng","doi":"10.1016/j.aquatox.2024.107224","DOIUrl":"10.1016/j.aquatox.2024.107224","url":null,"abstract":"<div><div>Plastic pollution, particularly microplastics and nanoplastics, poses a significant threat to marine ecosystems. Bivalves, vital filter feeders that accumulate plastic particles, underscore the necessity for advanced omics technologies to grasp their molecular reactions to plastic exposure.</div><div>This review delves into the impact of microplastics and nanoplastics on bivalves utilizing advanced omics technologies. Through an examination of omics data, this review sheds light on how bivalves react to plastic pollution, informing strategies for conservation and food safety. Furthermore, theoretical pathways have been formulated to decipher how bivalves respond to environmental stressors from microplastics or nanoplastics through the integration of diverse biological fields. In this review, we report that microplastics and nanoplastics in marine ecosystems primarily stem from human activities on land and in marine domains. Bivalves are negatively influenced by plastic contamination, impacting their health and economic worth. Exposure to plastic particles disrupts bivalve behavior, metabolism, and reproduction, precipitating health concerns. Integration of omics data is instrumental in unraveling molecular interactions and devising biomarkers for monitoring purposes. Ingestion of plastics by bivalves poses risks to human health. Additionally, mitigation tactics involve bans, levies, and advocating for biodegradable alternatives to curtail plastic pollution. The amalgamation of omics findings aids in the comprehension of bivalve responses and effectively addressing plastic pollution. Moreover, addressing plastic pollution necessitates a multidisciplinary approach encompassing scientific inquiry, regulatory frameworks, and collaboration with stakeholders. These strategies are paramount in safeguarding bivalves, marine ecosystems, food safety, and human health.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107224"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term exposure to sediment-associated antidepressants impacts life-history traits in an estuarine deposit-feeding worm","authors":"Martina Santobuono,&nbsp;Wing Sze Chan,&nbsp;Elettra D´Amico,&nbsp;Henriette Selck","doi":"10.1016/j.aquatox.2024.107189","DOIUrl":"10.1016/j.aquatox.2024.107189","url":null,"abstract":"<div><div>Hydrophobic pollutants, such as the antidepressant sertraline (SER), tend to sorb to particles in the water column and subsequently accumulate in the sediment. Long-term exposure to these pollutants may significantly affect sediment-dwelling organisms´ fitness and behavior. To address this knowledge gap, we investigated the impact of chronic exposure to a range of environmentally relevant and higher concentrations of sediment-associated SER on the deposit-feeding polychaete <em>Capitella teleta</em>. Since certain antidepressants can function as neurotoxic chemicals and endocrine disruptors on non-target species, we examined feeding rate and burrowing behavior in adult worms after 23 days of exposure (Experiment 1), and key life-history traits in juvenile worms during 35 days of exposure (Experiment 2) to sediment-associated SER (0.33 - 100 µg/g dw sediment). SER did not affect survival but reduced maturation and time to first reproduction: 37%, 50%, and 29% of the worms exposed respectively to SER 0.33, 3.3 and 33 µg/g reached maturation on day 21, whereas worms in the other treatments did not mature (0%; control) or reached a lower maturation degree (6%; 100 µg/g). Although not statistically significant, growth, feeding, and burrowing manifested non-monotonic trends: at environmentally relevant SER concentrations adults increased feeding and extended time to fully burrow into the sediment, and juveniles increased growth, whereas high concentrations had an inhibitory or no effect. Reproductive endpoints appeared most sensitive to chronic SER exposure. Even at low environmental concentrations, antidepressants can cause sublethal effects in non-target species, potentially affecting population dynamics and ecosystem functioning. Further research is key to fully understanding the ecological impact of hydrophobic chemicals in natural environments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107189"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating microplastic contamination in Omani mangrove habitats using large mud snails (Terebralia palustris)","authors":"Muna Al-Tarshi ,&nbsp;John Husband ,&nbsp;Sergey Dobretsov","doi":"10.1016/j.aquatox.2024.107220","DOIUrl":"10.1016/j.aquatox.2024.107220","url":null,"abstract":"<div><div>This study investigated microplastic pollution in the large mud snail <em>Terebralia palustris</em> (Linnaeus, 1767) (Gastropoda: Potamididae) inhabiting the <em>Avicennia marina</em> mangrove ecosystems along the Sea of Oman. A modified digestion protocol, combining two methods, was employed to improve the detection of microplastics within the snail tissue. Results indicated that 50 % of the examined snails contained microplastics, with significant variability observed among different lagoons. Snails from the polluted Shinas lagoon exhibited higher levels of microplastics compared to those from the lowest polluted Al-Qurum Natural Reserve (MPA). The most prevalent type of microplastic in snail tissues was fibers, making up 75.7 % of the total. Fragments constituted about 24.2 %. Using portable Raman spectrometry, Polyurethane (PU) was identified as the predominant polymer, accounting for 50 % of the total. This was followed by Acrylic and Polyethylene, each representing 18.75 %, and Polyethylene Vynil Acetate (PEVA) at 12.50 %. Overall, it is clear that while snails do reflect the presence of microplastics (MPs) in their environment, their physical attributes do not strongly correlate with the levels or types of MPs they contain. Additionally, the significant difference between the abundance of MPs in sediment and in snails illustrates that, while snails may serve as general indicators of microplastic pollution, they may not be reliable as precise bioindicators or sentinel species for quantifying the extent of this pollution. Further studies are needed to explore other potential bioindicators in mangrove habitats.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107220"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genes of filter-feeding species as a potential toolkit for monitoring microplastic impacts","authors":"Marta Pilar Ortiz-Moriano,&nbsp;Eva Garcia-Vazquez,&nbsp;Gonzalo Machado-Schiaffino","doi":"10.1016/j.aquatox.2024.107234","DOIUrl":"10.1016/j.aquatox.2024.107234","url":null,"abstract":"<div><div>Microplastics (MPs) are ubiquitous in the marine environment and impact organisms at multiple levels. Understanding their actual effects on wild populations is urgently needed. This study develops a toolkit to monitor changes in gene expression induced by MPs in natural environments, focusing on filter-feeding and bioindicator species from diverse ecological and taxonomic groups. Six candidate genes —Caspase, HSP70, HSP90, PK, SOD, and VTG— and nine filter-feeding species —two branchiopods, one copepod, five bivalves and one fish— were selected based on differential expression in response to MPs exposure (mainly the widely used polystyrene and polyethylene polymers) reported in over 30 publications. Some genes are particularly determinant, such as HSP70 and HSP90 (key to managing a wide range of stressors) and SOD (critical for addressing oxidative stress), as they are more directly related to stress. PK is related to carbohydrate metabolism (alterations in energy metabolism); VTG is associated with reproductive problems; Caspase mediates in apoptosis. Each gene in the toolkit plays a role depending on the type of stress assessed, and their combination provides a comprehensive understanding of the impacts of MPs. Differences in gene expressions between species and the exposure thresholds were found. These genes were examined in various scenarios with different types, concentrations, and sizes of MPs, alone or with other stressors. The toolkit offers significant advantages, allowing a comprehensive study of the impact of MPs and focusing on filtering bioindicator species, thus enabling pollution assessment and long-term monitoring. It will outperform traditional methods like tissue counts of MPs where only physical damage is visible, providing a deeper understanding. To our knowledge, this is the first toolkit of its kind.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107234"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct and transgenerational effects of simvastatin on the metabolism of the amphipod Gammarus locusta","authors":"João A. Rodrigues ,&nbsp;Raquel S. Chaves ,&nbsp;Miguel M. Santos ,&nbsp;Teresa Neuparth ,&nbsp;Ana M. Gil","doi":"10.1016/j.aquatox.2024.107221","DOIUrl":"10.1016/j.aquatox.2024.107221","url":null,"abstract":"<div><div>In this study, untargeted Nuclear Magnetic Resonance (NMR) metabolomics was applied for the first time, to our knowledge, to assess the metabolic impact of direct and transgenerational exposure (<em>F0</em> and <em>F3</em> generations, respectively) of amphipods <em>Gammarus locusta</em> to simvastatin (SIM), a pharmaceutical widely prescribed for the treatment of hypercholesterolemia. Results revealed the important gender-dependent nature of each of these effects. Directly exposed males showed enhanced glucose catabolism and tricarboxylic acid (TCA) cycle activity, in tandem with adaptations in osmotic regulation and glyoxylate metabolism. Exposed females exhibited only a small osmoregulatory effect. It is suggested that the response of exposed males may reflect reported high levels of methyl farnesoate hormone (low levels in females) and alterations in apical factors, namely decreased growth. Conversely, transgenerational effects were identified only in females, with impact on energy metabolism (glycolysis and TCA cycle enhancement) and osmoregulatory response. This expresses the ability of female gametes to transmit the effects of direct SIM exposure. Such effects were putatively related to reported delayed maturation and transcriptomic deviations impacting on carbohydrate and lipid metabolisms, possibly specifically engaging phenylalanine/tyrosine and choline in dopamine and choline metabolisms.</div><div>These findings reflect the importance of untargeted metabolomics in addressing not only direct exposure of contaminants, but also their transgenerational effects, potentially contributing towards improving hazard and risk assessment of biologically active compounds.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107221"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}