Distinctive lipidomic responses induced by polystyrene micro- and nano-plastics in zebrafish liver cells

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-02-24 DOI:10.1016/j.aquatox.2025.107291

Tiantian Wang , Miquel Perelló Amorós , Gemma Lopez Llao , Cinta Porte

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引用次数: 0

Abstract

Despite growing awareness of the size-dependent toxicity caused by micro- and nano-plastics (MNPs) in fish, the modulation of the liver lipidome as a function of particle size has not been thoroughly investigated. This study explores the subcellular and molecular responses induced by polystyrene microplastics (MPs, 1 µm) and nano-plastics (NPs, 52 nm) in zebrafish liver (ZFL) cells, with a focus on the modulation of the cell's lipidome and gene expression profiles. Both particle sizes are readily internalized by ZFL cells; however, NPs had a more pronounced impact compared to MPs. Lipidomic analysis revealed that MPs decreased polyunsaturated phospholipids, while NPs increased ether-linked phosphatidylcholines (PC-Ps/PCOs). Gene expression analysis showed that high concentrations of MPs down-regulated the expression of fatty acid synthesis related genes, and significantly downregulated the microsomal triglyceride transfer protein (mtp) gene, indicating a perturbation in lipid storage metabolism, which was not observed for NP exposure. In contrast, NPs induced a dose-dependent accumulation of lipids, suggesting increased lipid droplet formation and an activation of ceramide-mediated apoptosis pathway. These findings provide new insights into the molecular mechanisms of MNP toxicity and emphasize the importance of considering particle size when assessing environmental and health risks. Furthermore, this study highlights the potential of lipidomics for elucidating the mechanisms underlying MNP toxicity, prompting further research into of the long-term consequences of exposure.

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聚苯乙烯微塑料和纳米塑料在斑马鱼肝细胞中诱导的独特脂质组学反应

尽管越来越多的人意识到由微和纳米塑料（MNPs）引起的鱼类大小依赖性毒性，但肝脏脂质组作为颗粒大小的函数的调节尚未得到彻底的研究。本研究探讨了聚苯乙烯微塑料（MPs, 1µm）和纳米塑料（NPs, 52 nm）在斑马鱼肝脏（ZFL）细胞中诱导的亚细胞和分子反应，重点研究了细胞脂质组和基因表达谱的调节。两种粒径都容易被ZFL电池内化；然而，与MPs相比，NPs的影响更为明显。脂质组学分析显示，MPs降低了多不饱和磷脂，而NPs增加了醚连接磷脂酰胆碱（PC-Ps/PCOs）。基因表达分析显示，高浓度MPs下调了脂肪酸合成相关基因的表达，并显著下调了微粒体甘油三酯转移蛋白（mtp）基因的表达，表明脂质储存代谢受到干扰，而NP暴露并未观察到这一点。相反，NPs诱导了剂量依赖性的脂质积累，表明脂滴形成增加和神经酰胺介导的细胞凋亡途径的激活。这些发现为MNP毒性的分子机制提供了新的见解，并强调了在评估环境和健康风险时考虑颗粒大小的重要性。此外，本研究强调了脂质组学在阐明MNP毒性机制方面的潜力，促进了对暴露的长期后果的进一步研究。

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.