Aquatic Toxicology最新文献

{"title":"Low lipid levels caused by bisphenol S exposure trigger neuroinflammation and apoptosis in the brain of zebrafish","authors":"Zehui Du ,&nbsp;Shuai Li ,&nbsp;Hongyuan Peng ,&nbsp;Jiali Li ,&nbsp;Ze Li ,&nbsp;Shaoguo Ru ,&nbsp;Weiwei Wang","doi":"10.1016/j.aquatox.2025.107328","DOIUrl":"10.1016/j.aquatox.2025.107328","url":null,"abstract":"<div><div>Bisphenol S (BPS), as an environmental pollutant, is known to reduce brain lipid levels and induce neurotoxicity. However, whether brain lipid imbalance can induce neurotoxicity has not yet been clarified. Here, wild-type zebrafish and <em>apoEb</em> mutant zebrafish were used to investigate the effect of BPS on the macrophages proliferation and microglia mobilization caused by the decrease of cerebral lipids and its potential neurotoxic effects. The zebrafish exposed to BPS (1, 10, or 100 μg/L) from 2 hours after fertilization (hpf) to 3 days after fertilization (dpf) displayed microglial aggregation, as well as a decrease in brain lipid content. Lipidomic analyses of the brains and plasma of 50 dpf zebrafish exposed to BPS were used to identify key lipids, including lysophosphatidylcholine and phosphatidylcholine in brain and phosphatidylcholine in plasma. The <em>apoEb</em> mutant zebrafish as a hyperlipidemia model was used to further demonstrate that BPS-induced lipid reduction increased the number of microglia in the brain. Our data provide new insight into the mechanism by which pollutants cause neurotoxicity.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107328"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress and mitochondrial dysfunctions induced by cyanobacterial microcystin-LR in primary grass carp hepatocytes","authors":"Miao He ,&nbsp;Hui Wang ,&nbsp;Jianping Fu ,&nbsp;Jiming Ruan ,&nbsp;Fugui Li ,&nbsp;Ximei Liang ,&nbsp;Lili Wei","doi":"10.1016/j.aquatox.2025.107327","DOIUrl":"10.1016/j.aquatox.2025.107327","url":null,"abstract":"<div><div>Microcystin-LR (MC-LR), a cyclic heptapeptide produced by freshwater cyanobacteria, induces a range of liver injuries. However, the mechanisms underlying MC-LR toxicity in primary hepatocytes of aquatic organisms remains poorly understood. In this study, we investigated the effects of MC-LR on oxidative stress and mitochondrial function using primarily cultured grass carp hepatocytes. The results revealed that IC₅₀ of MC-LR on grass carp primary liver cells for 24 hours was 2.40 μmol/L. Based on 24h-IC₅₀, concentrations of 0, 0.30, 0.60, and 1.20 μmol/L were used in subsequent experiments. MC-LR exposure led to a significant reduction in cell viability, induced abnormal cell morphology, and caused plasma membrane rupture, as indicated by elevated LDH activity in a concentration-dependent manner. Additionally, MC-LR exposure induced oxidative stress, resulting in increased ROS levels and downregulation of genes associated with oxidative stress, including <em>keap1, nrf2, cat, sod1, gpx, gst</em>, and <em>gr</em> (<em>P</em>&lt;0.05). Furthermore, the electron microscopy results showed that MC-LR caused damage to the ultrastructure of primary hepatocytes, including mitochondrial membrane rupture, vacuolation, and induction of mitochondrial autophagy. Moreover, MC-LR exposure elevated intracellular Ca²⁺ concentration, reduced MMP and ATP levels, and inhibited mitochondrial respiratory chain complex I activity (<em>P</em>&lt;0.05). qRT-PCR analysis demonstrated that MC-LR treatment significantly decreased the transcriptional levels of genes related to mitochondrial quality control including <em>pgc-1α, tfam, nrf1, drp1, opa1, mfn1</em>, and <em>mfn2</em> (<em>P</em>&lt;0.05). Collectively, our findings highlight that MC-LR causes oxidative stress and impairs mitochondrial function, leading to further hepatocyte damage, which provides insights into the mechanisms of MC-LR-induced hepatotoxicity and offers valuable references for further investigations.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107327"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the immunotoxic effects of benzo[a]pyrene on Mytilus coruscus through histopathological, enzymatic, and transcriptomic analyses","authors":"Xiaoya Wang ,&nbsp;Yaru Li ,&nbsp;Kaida Xu ,&nbsp;Qingyang Li ,&nbsp;Shuang Yan ,&nbsp;Yingying Ye ,&nbsp;Pengzhi Qi ,&nbsp;Hongfei Li","doi":"10.1016/j.aquatox.2025.107326","DOIUrl":"10.1016/j.aquatox.2025.107326","url":null,"abstract":"<div><div>Benzo[a]pyrene (BaP) is a representative polycyclic aromatic hydrocarbon (PAH) known for its significant toxicity and environmental persistence, capable of causing mutations, deformities, and cancer in aquatic organisms. However, systematic studies on the effects of BaP exposure on histological damage, cell apoptosis, enzyme activity changes, and gene expression in <em>Mytilus coruscus</em> (M<em>. coruscus</em>), an important ecological indicator species, remain scarce. In this study, the biological effects of BaP on M<em>. coruscus</em> and the immunotoxic mechanisms following BaP exposure were evaluated using histological analysis, TUNEL assay, enzyme activity assays, and transcriptome sequencing. Our findings revealed notable histopathological changes due to BaP exposure, including hemocyte infiltration, atrophy, and deformation of digestive tubules in the digestive glands, as well as epithelial cell detachment and deformation in gills. Antioxidant enzyme activities (CAT, GSH-Px, SOD, T-AOC) varied significantly across tissues under BaP stress. Additionally, significant DNA fragmentation and increased apoptosis were observed in BaP-exposed groups compared to controls. Transcriptome analysis showed that after BaP exposure, nucleotide excision repair and innate immune response pathways were suppressed, while the metabolism of xenobiotics by cytochrome P450, glutathione biosynthesis, and apoptosis pathways were upregulated. These results elucidate the toxic mechanisms of BaP on M<em>. coruscus</em> and the immunotoxic responses of the mussels. This study enhances our understanding of how BaP and similar pollutants affect marine bivalves, providing valuable insights for environmental monitoring and pollutant management strategies.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107326"},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pups on mercury: Tracking early life exposure on South American fur seals (Arctocephalus australis) and South American sea lions (Otaria byronia) in the Southeastern Pacific","authors":"I. Peña-Galindo ,&nbsp;B. Bernales-Santolaya ,&nbsp;F. Montalva ,&nbsp;J. Gutiérrez ,&nbsp;F. Quesada-Alvarado ,&nbsp;S. Navarrete-Quintanilla ,&nbsp;V. Robbins ,&nbsp;D.J. Perez-Venegas ,&nbsp;G. Cortés-Hinojosa ,&nbsp;M. Seguel ,&nbsp;G. Chiang","doi":"10.1016/j.aquatox.2025.107325","DOIUrl":"10.1016/j.aquatox.2025.107325","url":null,"abstract":"<div><div>Mercury exists in three forms: elemental, inorganic, and organic, with methylmercury (MeHg) being the most concerning due to its ability to cross cellular barriers and bioaccumulate, particularly in marine mammals, where over 90 % of total mercury is in the MeHg form. Despite its importance, there is limited data on mercury bioaccumulation in marine mammals and maternal transfer mechanisms in the Southern Hemisphere. Pinnipeds, as ocean sentinels, are valuable for monitoring contaminants due to their ecological and biological traits. This study investigates mercury burdens and maternal transfer of mercury in South American sea lions (SASL) and South American fur seals (SAFS). Samples of clots and vibrissae from SASL pups and clots and milk from SAFS pups and females were analyzed. Total mercury (THg) levels in SASL ranged from 8.36 to 305.43 μg/Kg w.w. in clots and from 3071.8 to 28,034.5 μg/Kg d.w. in vibrissae. In SAFS, THg levels in clots ranged from 0.40 to 358.77 μg/Kg w.w. and in milk from 3.4 to 14.1 μg/Kg w.w. Significant differences were observed between newborn pups of both species, with a positive correlation between THg levels in clots from SAFS pups and females and between clots and vibrissae in SASL pups, indicating maternal transfer during gestation. Additionally, THg levels in SAFS pups decreased over time, suggesting biodilution. These species allow us to have a long term monitoring in both colonies and two different areas in Chile and are relevant findings to food security and the treatment of heavy metal contamination.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107325"},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicokinetic and liver proteomic study of the Chinese rare minnow (Gobiocypris rarus) exposed to F-53B","authors":"Hongbo Yang ,&nbsp;Chan Xu ,&nbsp;Jieyu Song ,&nbsp;Jin Li ,&nbsp;Chan Zhang ,&nbsp;Chunli Teng ,&nbsp;Kai Ma ,&nbsp;Feng Xie","doi":"10.1016/j.aquatox.2025.107312","DOIUrl":"10.1016/j.aquatox.2025.107312","url":null,"abstract":"<div><div>Perfluorooctane sulfonic acid (PFOS) and its alternative 6:2 chlorinated polyfluoroethersulfonate (6:2 Cl-PFESA, also known as F-53B), are frequently detected in a variety of environmental and human samples. These substances have been associated with hepatotoxic effects, including disorders in lipid metabolism and oxidative stress. However, the molecular mechanisms underlying the causal relationship between exposure to F-53B and hepatotoxicity remain inadequately understood. This study investigated the toxicokinetics and mechanisms of hepatotoxicity associated with prolonged exposure to F-53B in adult Chinese rare minnows. Specifically, 5-month-old adult Chinese rare minnow was exposed to concentrations of 10 μg/L and 200 μg/L of F-53B for a duration of 28 days for bioaccumulation assessment, followed by a 14-day period for metabolic evaluation. The findings indicated that the bioaccumulation of F-53B in the tissues was positively correlated with the exposure concentrations. The logarithmic bioconcentration factor (Log BCF28d) was determined to be 2.67 ± 0.02 for the low concentration group and 2.27 ± 0.01 for the high concentration group. The calculated half-lives (t_1/2) were 18.50 ± 1.67 days and 21.38 ± 0.31 days for the respective concentration groups. F-53B protein exhibited a distinct tissue-specific distribution in adult Chinese rare minnow, with the following order of enrichment: Blood &gt; Liver &gt; Gonad &gt; Gill &gt; Intestine &gt; Brain &gt; Muscle. F-53B was primarily concentrated in the blood and liver, where the protein content was significantly higher. Exposure to F-53B for 28 days significantly elevated biochemical levels associated with lipid metabolism and increased the activities of the enzymes FAS, PPARα, and ACC in the liver. This exposure also resulted in impairment of the hepatic oxidative system in the Chinese rare minnow, with F-53B significantly reducing most of the measured markers related to oxidative stress (e.g., GSH, SOD, CAT, and MDA). Proteomic analysis indicated that the toxicity of F-53B regulates the expression of proteins across several functional classes. Based on the functional information of the differential proteins provided in UniProt and KEGG, they were categorized into five main categories: Cellular Processes, Environmental Information Processing, Genetic Information Processing, Metabolism, and Organismal Systems. This study indicate that F-53B is bioaccumulative and persistent in Chinese rare minnow, and can further induce oxidative stress and lipid metabolism disorders. Combined with proteomic research methods, the toxicological effects of F-53B on Chinese rare minnow can be better explained.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107312"},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics study reveals DON-induced intestinal toxicity in adult zebrafish through disruption of amino acid metabolism and sphingolipid signaling pathway","authors":"Yuxiang Wang ,&nbsp;Luhan Wang ,&nbsp;Yaowen Du ,&nbsp;Feng Yao ,&nbsp;Miaomiao Zhao ,&nbsp;Chenggang Cai ,&nbsp;Ruiyu Zhu ,&nbsp;Suqin Shao","doi":"10.1016/j.aquatox.2025.107324","DOIUrl":"10.1016/j.aquatox.2025.107324","url":null,"abstract":"<div><div>Deoxynivalenol (DON), a prevalent mycotoxin contaminating cereal crops globally, poses significant threats to animal and human health through its gastrointestinal toxicity. While DON-induced intestinal damage has been documented in mammals, its metabolic mechanisms in aquatic species remain poorly understood, particularly in adult zebrafish models that offer unique advantages for toxicological studies. Multi-omics analysis revealed 16 key differential metabolites (9 upregulated, 7 downregulated) associated with amino acid metabolism and carbohydrate homeostasis. Pathway enrichment analysis identified significant perturbations in 2-oxocarboxylic acid metabolism and sphingolipid signaling, suggesting mitochondrial dysfunction and epithelial barrier disruption as primary toxicity mechanisms. This study establishes the first adult zebrafish model for DON intestinal toxicity evaluation, demonstrating its utility in revealing conserved metabolic targets across species. The identified pathway-specific biomarkers provide novel insights for developing dietary interventions against mycotoxin exposure.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107324"},"PeriodicalIF":4.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the marine medaka AHRs and the comparison with those of Japanese medaka in response to dioxin and additional AHR ligands","authors":"Wanglong Zhang ,&nbsp;Yanjiao Zong ,&nbsp;Ruize Sun ,&nbsp;Zhenhong Xue ,&nbsp;Wenhui Wan ,&nbsp;Anran Ren ,&nbsp;Yongchao Ma ,&nbsp;Wenjing Tian ,&nbsp;Renjun Wang","doi":"10.1016/j.aquatox.2025.107323","DOIUrl":"10.1016/j.aquatox.2025.107323","url":null,"abstract":"<div><div>The global water pollution now calls for precise risk assessment of chemicals, e.g., dioxins and the dioxin-like compounds (DLCs). The freshwater and marine medaka have been widely implemented in the toxicity testing, and perhaps give mechanistic information for comparative biology. The question that ‘will they report equal results due to their close phylogenetic relation’ has been raised, therefore, we explored their physiological and molecular responses to dioxin. As the mediator of the dioxin toxicity, the aryl hydrocarbon receptor (AHR) of marine medaka (<em>Oryzias melastigma</em>) has not been functionally characterized and might be species-specific. In terms of sensitivity to dioxin—2,3,7,8-tetrachlorodibenzo-<em>p</em>-dioxin (TCDD), the EC₅₀ values of omeAHR1a (0.16±0.12 nM), omeAHR1b (2.96±2.96 nM), omeAHR2a (0.44±0.30 nM), and omeAHR2b (9.00±6.88 nM) exhibit marked variations. The omeAHR2a and omeAHR1a display heightened sensitivity compared to the freshwater Japanese medaka (<em>Oryzias latipes</em>) counterparts olaAHR2a and olaAHR1a, respectively. The results indicate the in vitro sensitivity of AHR among species can vary by one or two orders of magnitude. Further mechanistic investigations using additional ligands and computational modeling reveal that: 1) most of omeAHR2a, olaAHR2a, dreAHR2, and hsaAHR interact with ligands in the affinity order of TCDD &gt; PCB126 &gt; BNF &gt; indole, mirroring their AHR transactivation potency, but the docking poses and dynamics can vary; 2) one AHR subform's high sensitivity to dioxin—TCDD may extend to DLCs but not to other types of ligands. Beyond the in vitro study, the preliminary in vivo LC₅₀ data indicate that marine medaka (LC₅₀: 1.64 ng/L (95 % CI: 1.05–2.55 ng/L)) has similar sensitivity, and possibly slightly greater (not statistically determined yet), to TCDD in comparison with Japanese medaka (LC₅₀: 3.42 ng/L (95 % CI: 1.37–6.48 ng/L)).These insights underscore the difference of AHR biology among species even the close relative species, and point out the necessity for meticulous consideration when evaluating the toxicity of compounds and when extending predictive toxicity assessments to more species.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107323"},"PeriodicalIF":4.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurotoxicity and aggressive behavior induced by anesthetic etomidate exposure in zebrafish: Insights from multi-omics and machine learning","authors":"Xuewei Li ,&nbsp;Xuhui Lin ,&nbsp;Zheng Zhang ,&nbsp;Zile Zhuang ,&nbsp;Yihan Li ,&nbsp;Yuxuan Luo ,&nbsp;Yupeng Pan ,&nbsp;Qizhi Luo ,&nbsp;Xuncai Chen","doi":"10.1016/j.aquatox.2025.107321","DOIUrl":"10.1016/j.aquatox.2025.107321","url":null,"abstract":"<div><div>Etomidate (ETO), widely employed as a surgical anesthetic and more recently recognized as a drug of abuse, has been frequently detected in aquatic environment. However, the toxicity assessment of ETO is insufficient. Adult zebrafish were used to investigate toxicological effects of ETO. Four weeks ETO exposure could induced abnormal behaviors, including reduced anxiety, memory impairment, and heightened aggression. The increased aggression was quantitatively characterized using machine learning, which revealed significantly elevated instantaneous velocity and drastic changes in angular velocity. ETO was predominantly accumulated in the zebrafish brain, where it binds to GABA-A receptors, leading to a significant increase in GABA content. Furthermore, fluorescent staining of reactive oxygen species (ROS) in the brain revealed that ETO exposure significantly increased the oxidative stress level. This oxidative stress resulted in mitochondrial swelling, rupture, and damage to myelinated nerve fibers, ultimately causing cerebral injury in zebrafish. Multi-omics analysis further elucidated that ETO exposure down-regulated the MAPK signaling pathway, hyperactivated motor proteins, and induced metabolic disorders of lipids and amino acids. In summary, this study demonstrates that ETO induces neurotoxicity and behavioral alterations in zebrafish. These findings provide a critical insight into the mechanisms underlying ETO's neurotoxic effects and contribute to a more comprehensive understanding of its environmental and health risks.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107321"},"PeriodicalIF":4.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community-level effects of a neonicotinoid pesticide on the metabolism of freshwater microorganisms","authors":"Alessandra CERA ,&nbsp;Sakie KODAMA ,&nbsp;Leanne K. FAULKS ,&nbsp;Hiroshi HAKOYAMA","doi":"10.1016/j.aquatox.2025.107311","DOIUrl":"10.1016/j.aquatox.2025.107311","url":null,"abstract":"<div><div>This study provides an ecotoxicological assessment of the effects of the neonicotinoid dinotefuran on freshwater microorganisms. Epilithic biofilm was sampled from a small stream and exposed to dinotefuran for 14 days in Biolog EcoPlates. In general, we found that a concentration of 0.100 mg <span>l</span>^-1 of dinotefuran induced the microorganism community to catabolise carbon sources faster. However, catabolic activity varied depending on the type of carbon source. Catabolism increased for Putrescine (+4673 %), Serine (+376 %), Galacturonic Acid (+206 %), Pyruvic Acid Methyl Ester (+177 %), and Gamma Amino Butyric Acid (+113 %); and decreased for Arginine (-59 %), Asparagine (-26 %), and Mannitol (-21 %). This is the first study in which EcoPlates have been used to investigate the effects of dinotefuran on freshwater epilithic biofilm at an environmentally realistic concentration.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107311"},"PeriodicalIF":4.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity of microplastics polystyrene to freshwater planarians and the alleviative effects of anthocyanins","authors":"He-Cai Zhang,&nbsp;Xiao-Qing Yang,&nbsp;Cai-Hui Wang,&nbsp;Chang-Yang Shang,&nbsp;Chang-Ying Shi,&nbsp;Guang-Wen Chen,&nbsp;De-Zeng Liu","doi":"10.1016/j.aquatox.2025.107310","DOIUrl":"10.1016/j.aquatox.2025.107310","url":null,"abstract":"<div><div>It is impossible to overlook the effects of microplastics (MPs) on aquatic organisms as they continuously accumulate in water environment. Freshwater planarians, which exist in the benthic zone of water bodies and come into contact with the deposited MPs particles, provide a highly representative model for studying the effects of MPs on aquatic organisms. Anthocyanins (ANTs) have gained significant popularity in recent years for their diverse health benefits. In the current study, the median lethal concentration (LC₅₀) of polystyrene (PS) to planarian <em>Dugesia japonica</em> was determined for the first time. Based on this, multiple toxic effects of single PS and PS in combination with ANTs on planarians were explored. The results showed that PS exposure disrupted the redox homeostasis and induced oxidative damage in planarians. Also, PS stress affected the neuromorphology, aggravated cell apoptosis in planarians probably by altering neural gene expressions as well as promoting the expression of apoptosis-related genes while inhibiting stem cell marker genes. In addition, the results also suggested that co-exposure of ANTs could effectively alleviate the toxicity of PS on planarians. Particularly, long-term environmentally relevant concentration PS exposure exhibited a higher propensity for inducing toxicity on planarians than short-term high concentration acute exposure, indicating that the harm of environmental MPs to humans and wildlife exposed to them should not be underestimated. Therefore, considering the recently rising and rapid development of ecotoxicomics, more in-depth research on the toxicity mechanism of environmentally relevant concentration PS-MPs to freshwater planarians from multi-omics levels will be our future work.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107310"},"PeriodicalIF":4.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}