Aquatic Toxicology最新文献

{"title":"Co-exposure to polystyrene nanoplastics and mercury synergistically exacerbates toxicity in rare minnow (Gobiocypris rarus) compared to individual exposures","authors":"Tingting Chu ,&nbsp;Bo Xu ,&nbsp;Fuyu Guo ,&nbsp;Meng Zhu ,&nbsp;Ruyi Yang","doi":"10.1016/j.aquatox.2025.107416","DOIUrl":"10.1016/j.aquatox.2025.107416","url":null,"abstract":"<div><div>Nanoplastics (NPs) and mercury (Hg) are ubiquitous pollutants that co-occur in aquatic ecosystems. However, the interaction between NPs and Hg, particularly whether NPs affect the accumulation and <em>in vivo</em> biotransformation of Hg in aquatic organisms, remains unclear. The toxicity of NPs and mercuric chloride (HgCl₂), both individually and in combination at environmentally relevant concentrations, on rare minnow (<em>Gobiocypris rarus</em>) were investigated in this study. The results demonstrated that NPs increased total Hg accumulation by 33.33 % but had limited effects on methylmercury (MeHg) content and its proportional distribution in muscle tissue compared to single Hg exposure. Both NPs and Hg induced significant growth inhibition, intestinal damage, oxidative stress, and inflammatory responses in rare minnow, with endpoint-specific effect patterns. Moreover, NPs and Hg dramatically altered gut microbiota composition and co-occurrence networks, with NPs inducing more metabolic pathway changes than Hg. Notably, combined exposure exacerbated almost all toxic effects in rare minnow compared to individual exposures, indicating synergistic interactions between NPs and Hg. These findings highlight the need to consider co-existing contaminants when evaluating NP toxicity.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107416"},"PeriodicalIF":4.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological risks under combined pollution: Toxicological effects of clothianidin and microplastics on Penaeus vannamei","authors":"Zhi Luo ,&nbsp;Zhi-Yu Lin ,&nbsp;Zhen-Fei Li ,&nbsp;Zhen-Qiang Fu ,&nbsp;Feng-Lu Han ,&nbsp;Er-Chao Li","doi":"10.1016/j.aquatox.2025.107417","DOIUrl":"10.1016/j.aquatox.2025.107417","url":null,"abstract":"<div><div>In real-world conditions, multiple pollutants coexist, making it insufficient to study a single pollutant's effects. Clothianidin, a widely present neonicotinoid insecticide, poses significant ecological risks due to its unique mechanism of action. Similarly, microplastics, another prevalent pollutant, have notable ecological impacts. This study aims to assess the ecotoxicological effects of clothianidin and microplastics on <em>P. vannamei</em> over 28 days, both individually and in combination. The study found that combined exposure significantly inhibited the shrimp's weight gain rate and hepatosomatic index, and significant changes were observed in the shrimp's immune defense, signal transmission, and energy metabolism. Key findings include changes in levels of hemocyanin, respiratory burst, nitric oxide, and phenol oxidase, as well as disturbances in enzyme activities of Lactate dehydrogenase, NADPH-dependent isocitrate dehydrogenase, and Succinate dehydrogenase. Additionally, fluctuations in levels of acetylcholine, dopamine, and acetylcholinesterase confirmed disruptions in neural signaling. Transcriptomic analysis further revealed the profound impact of these pollutants on gene expression and metabolic processes in the hepatopancreas and nervous system. This comprehensive assessment highlights the potential impact on shrimp growth and emphasizes the ecological risks of clothianidin and microplastics, providing insights for future risk assessments and the identification of biomarkers.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107417"},"PeriodicalIF":4.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sub-chronic nanoplastic toxicity in Etroplus suratensis (Pisces, Cichilidae): Insights into tissue accumulation, stress and metabolic disruption","authors":"Bini C. Das ,&nbsp;Devika Pillai ,&nbsp;Rejish Kumar V․J","doi":"10.1016/j.aquatox.2025.107418","DOIUrl":"10.1016/j.aquatox.2025.107418","url":null,"abstract":"<div><div>Nanoplastics, as widespread and persistent pollutants, pose a significant threat to the health of organisms. In this study, the impacts of polystyrene nanoplastics on the euryhaline fish, pearl spot (<em>Etroplus suratensis</em>) were investigated. Pearl spot were exposed to 0, 0.2, 2, and 4 mg/L of PS NPs for 14 days and NP accumulation, as well as its impact of accumulation on biochemical and oxidative stress parameters and gene expression were examined. The NP accumulation pattern was different at different exposure concentrations. At 0.2 mg/L of NP exposure concentration, the accumulation order observed was gills ˃ liver ˃ muscle ˃ intestine ˃ brain ˃ spleen. However, at higher exposure concentrations (2 and 4 mg/L), the accumulation order changed to intestine ˃ liver ˃ gills ˃ muscle ˃ spleen ˃ brain. Moreover, the accumulation led to considerable variations in biochemical parameters. Glucose, total cholesterol, SGOT, SGPT, and ALP levels increased, while albumin, total protein, and A/G ratio decreased due to NP exposure. Additionally, the antioxidant levels including SOD, CAT, GPx, and TAC, were remarkably reduced. This could explain the higher levels of MDA and PC, as well as the reduced expression of <em>NRF2</em> and <em>P53</em> in the NP-exposed groups, indicating oxidative damage. The significant increases in cortisol levels and the up-regulation of <em>HSP70</em> indicate that fish experience stress evoked by NP. The NP exposure reduced the <em>IGF1</em> and <em>CYP1A</em> expression, indicating its potential to impair growth and xenobiotic metabolism. These findings indicate that NPs induce stress, biochemical changes, oxidative damage, inhibited growth, and metabolism disruption in fish. This study is the first to examine the environmentally relevant NPs concentrations on protein damage from oxidative stress, toxic metabolism, and the expression of <em>NRF2, P53, IGF1</em>, and <em>CYP1A</em> in a Cichlid fish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107418"},"PeriodicalIF":4.1,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity effects and mechanisms of graphdiyne towards freshwater microalgae Scenedesmus obliquus","authors":"Wei Hong ,&nbsp;Fan Zhang ,&nbsp;Zhuang Wang","doi":"10.1016/j.aquatox.2025.107419","DOIUrl":"10.1016/j.aquatox.2025.107419","url":null,"abstract":"<div><div>Graphdiyne (GDY), an emerging 2D carbon nanomaterial, holds promise yet remains unexplored for environmental hazards. Herein, we investigated the toxicity effects and mechanisms of GDY towards the freshwater microalga <em>Scenedesmus obliquus</em> at environmentally relevant concentrations. The results revealed that 1 mg/L of GDY inhibited the growth of the algae and significantly induced a decrease in photosynthetic pigments. Exposure to 0.1 and 1 mg/L of GDY led to a reduction in cell membrane permeability and induced intracellular oxidative stress in the algae. Further, 1 mg/L of GDY caused oxidative damage to the algal cells. Molecular modeling indicated that GDY could directly affect the stability of dsDNA fragment. Transcriptome analysis showed that GDY at 1 mg/L influenced the expression of 2216 genes, with the glycerolipid metabolism pathway being enriched significantly. Metabolome analysis identified 129 differentially expressed metabolites (DEMs) in the algae exposed to 1 mg/L of GDY, revealing three significantly disrupted pathways: glycerophospholipid metabolism, pentose phosphate pathway, and flavonoid biosynthesis. The integrated transcriptome and metabolome analysis suggested that GDY exposure downregulated the level of a lysophosphatidylcholine species (LPC 18:3) by inhibiting the expression of genes related to FMN binding molecular functions. Simultaneously, the algae responded to GDY exposure by upregulating genes involved in the biological process of carbohydrate metabolic process and the molecular function of hydrolase activity, as well as increasing the levels of DEMs, specifically a lysophosphatidic acid species (LPA 16:4). This study elucidates the stress mechanisms induced by GDY in aquatic organisms and emphasizes the importance of monitoring its acute biological effects.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107419"},"PeriodicalIF":4.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life cycle toxicity evaluation of coated magnetite nanoparticles to the amphipod Hyalella curvispina","authors":"Nuria Guadalupe Espert ,&nbsp;Jorgelina Luján Villanova ,&nbsp;Sofía Ayelén Vedelago ,&nbsp;Mariana Noelia Mardirosian ,&nbsp;Malena Morell ,&nbsp;Cecilia Inés Lascano ,&nbsp;Andrés Venturino","doi":"10.1016/j.aquatox.2025.107406","DOIUrl":"10.1016/j.aquatox.2025.107406","url":null,"abstract":"<div><div>The presence of organic pollutants in aquatic environments requires effective solutions to reduce their concentration. In this context, the development of environmental remediation technologies using nanomaterials has emerged as a promising alternative. However, prior to their application, it is necessary to evaluate the toxicity of these materials along their lifecycle. The aim of this study was to analyze the acute toxicity and behavioral alterations triggered by exposure to magnetite nanoparticles coated with oleic acid (NPOA), to anthracene (ANT) as a model contaminant to be removed, and to the different remaining products after a simulated NPOA remediation procedure of ANT-contaminated water in the native amphipod <em>Hyalella curvispina</em>. Our results show no significant mortality with ANT up to 2 mg/L; however, behavioral alterations were observed from 24-h exposure, but they diminished over time along with ANT media concentration. A trend towards increased mortality with rising concentrations of NPOA was observed up to 10 mg/L, and stabilized between 25 and 100 mg/L. Additionally, NPOA exposure caused behavioral alterations that increased with concentration and remained along the 96 hours of the bioassay. The combined assays showed no significant differences between the combined NPOA-ANT, the remaining water post-remediation and the control. In conclusion<em>,</em> neither ANT and NPOA, individually or combined, were lethal for adult <em>H. curvispina</em> individuals. Nonetheless, they impacted behavior causing stillness, altered pleopodal locomotion frequencies and decreased response to stimuli, which may affect their survival in the environment.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107406"},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic tributyltin exposure induces metabolic disruption in an invertebrate model animal, Lymnaea stagnalis","authors":"István Fodor ,&nbsp;János Schmidt ,&nbsp;Réka Svigruha ,&nbsp;Zita László ,&nbsp;László Molnár ,&nbsp;Sándor Gonda ,&nbsp;Károly Elekes ,&nbsp;Zsolt Pirger","doi":"10.1016/j.aquatox.2025.107404","DOIUrl":"10.1016/j.aquatox.2025.107404","url":null,"abstract":"<div><div>Over the last 20 years, tributyltin (TBT) has been reported to cause metabolic disruption in both invertebrates and vertebrates, highlighting the need for further detailed analysis of its physiological effects. This study aimed to investigate the metabolic-disrupting effects of TBT from the behavioral to the molecular level. Adult specimens of the great pond snail (<em>Lymnaea stagnalis</em>) were exposed to an environmentally relevant concentration (100 ng L^-1) of TBT for 21 days. After the chronic exposure, behavioral alterations as well as histological, cellular, and molecular changes were investigated in the central nervous system, kidney, and hepatopancreas. TBT exposure significantly decreased feeding activity, while locomotor activity remained unchanged. At the histological level, the cellular localization of tin was demonstrated in all tissues investigated and, in addition, characteristic morphological changes were observed in the kidney and hepatopancreas. Tissue-specific changes in lipid profiles confirmed TBT-induced disruption of lipid homeostasis in mollusks, characterized by a consistent reduction in the proportion of polyunsaturated fatty acids and a shift toward more saturated lipids. The expression of 17β-hydroxysteroid dehydrogenase type 12 (HSD17B12) enzyme, involved in lipid metabolism in vertebrates, was reduced in all three tissues after TBT exposure. Our results show that TBT induces significant multi-level metabolic changes in <em>Lymnaea</em>, including direct alterations in feeding activity and lipid composition. Our findings also suggest that HSD17B12 enzyme plays a key role in lipid metabolism in mollusks, as in mammals, and is likely involved in TBT-induced metabolic disruption. Overall, our study extends the findings of previous studies on mollusks by providing novel behavioral as well as tissue-specific histological and metabolic data and highlights the complexity and evolutionary conserved way of TBT-induced metabolic disruption.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107404"},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MCPA-Na exposure in aquatic systems: disruption of pathways and increased susceptibility to infection in fish","authors":"Yanxia Lin ,&nbsp;Ran Shi ,&nbsp;Mengzhen Wang ,&nbsp;Yali Wang ,&nbsp;Yunfan Han ,&nbsp;Yongcui Ma ,&nbsp;Liyin Li ,&nbsp;Xiaohua Xia","doi":"10.1016/j.aquatox.2025.107405","DOIUrl":"10.1016/j.aquatox.2025.107405","url":null,"abstract":"<div><div>MCPA-Na (2-methyl-4-chlorophenoxyacetic acid) is a selective herbicide widely used in agricultural cultivation. Despite monitoring indicating risks to aquatic life, the specific organ effects and pathogen susceptibility are unclear. Therefore, we constructed a “compound-core target-signaling pathway” network using network toxicology methods, and the results showed that MCPA-Na interacted with multiple organs of loach (including intestine, liver, kidney, heart, gills, skin and blood). STRING and Cytoscape software were used to screen the core targets: PPAR (Peroxisome proliferator-activated receptor), ACE (angiotensin converting enzyme), REN (Renin), and CA9 (carbonic anhydrase). KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that the core targets of each tissue were significantly enriched in the renin-angiotensin system, NF-κB signaling pathway, adherens junctions and cholinergic synapses. The relationship between the toxicology and molecular markers of MCPA-Na was further explored by using animal experiments, and the susceptibility of <em>Misgurnus anguillicaudatus</em> (loach) to opportunistic pathogens after toxic exposure was simulated by using opportunistic pathogen challenge <em>Aeromonas hydrophila</em> (<em>A. hydrophila</em>). It was found that the compound induced oxidative stress and triggered intestinal inflammation and promoted apoptosis. These processes undermine the intestinal barrier and increase the susceptibility of loach to the <em>A. hydrophila</em>, thereby exacerbating the challenge of aquaculture food safety.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107405"},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxic effects of anionic polyacrylamide on the developmental stages of Oryzias melastigma embryos and larvae","authors":"Xinya Zhao ,&nbsp;Jiangwei Zan ,&nbsp;Zhaohui Sun ,&nbsp;Xiangping Xue ,&nbsp;Hai Ren ,&nbsp;Huiru Fu ,&nbsp;Fei Si ,&nbsp;Xiaomin Jin","doi":"10.1016/j.aquatox.2025.107402","DOIUrl":"10.1016/j.aquatox.2025.107402","url":null,"abstract":"<div><div>Anionic Polyacrylamide (APAM) is widely used in oil extraction processes, serving as an oil-repellent polymer and constituting a critical component of water-based drilling fluids. The environmental and ecological effects of APAM on fishery resources have attracted significant attention, yet its toxic mechanism in marine fish at early developmental stages remains poorly understood. The potential effects of APAM on marine medaka (<em>Oryzias melastigma</em>) embryos were investigated by exposing them to 0, 120, 240, 480, and 960 mg/L for 18 d. APAM exposure caused developmental toxicity in embryos, leading to reduced heart rates, delayed and decreased hatching, increased mortality and malformations. The activities of superoxide dismutase (SOD) and catalase (CAT) initially increased after 2 d of exposure but decreased after 8 and 18 days of prolonged stress, while malondialdehyde (MDA) concentration increased, causing lipid peroxidation and worsening oxidative damage. After 18 days of APAM exposure, low and medium concentrations increased the expression of cardiovascular genes GATA4 and NKX2.5, while high concentrations decreased NKX2.5, leading to heart defects like elongated hearts and pericardial cysts. Additionally, low concentrations significantly boosted nervous system genes SHHA and SYN2A, enhancing swimming behaviors, whereas high concentrations suppressed these genes, reducing swimming activity. In conclusion, this study demonstrated that APAM exposure causes developmental toxicity, oxidative stress, neurotoxicity, and disrupts early cardiac development in <em>O. melastigma</em> embryos, providing insight into its toxic effects on early marine fish development.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107402"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatotoxic effects of exposure to different concentrations of Dibutyl phthalate (DBP) in Schizothorax prenanti: Insights from a multi-omics analysis","authors":"Luo Lei ,&nbsp;Wuga Sha ,&nbsp;Qing Liu ,&nbsp;Shidong Liu ,&nbsp;Yinhua Zhou ,&nbsp;Rundong Li ,&nbsp;Yuting Duan ,&nbsp;Suxing Fu ,&nbsp;Hejiao Li ,&nbsp;Rongrong Liao ,&nbsp;Linzhen Li ,&nbsp;Rongzhu Zhou ,&nbsp;Chaowei Zhou ,&nbsp;Haiping Liu","doi":"10.1016/j.aquatox.2025.107390","DOIUrl":"10.1016/j.aquatox.2025.107390","url":null,"abstract":"<div><div>Dibutyl phthalate (DBP) is one of the most widely used phthalate esters (PAEs) that raise increasing ecotoxicological concerns due to their harmful effects on living organisms and ecosystems. Recently, while PAEs pollution in the Yangtze River has attracted significant attention, little research has been conducted on the impact of PAEs stress on <em>S. prenanti</em>, an endemic and valuable species in the Yangtze River. In this study, one control group (C-L) and three experimental groups: T1-L (3 µg/L), T2-L (30 µg/L), and T3-L (300 µg/L) were established with reference to the DBP concentration in the environment. For the first time, we investigated the effects of DBP stress on the liver of <em>S. prenanti</em> using histomorphological, physiological, and biochemical indexes, as well as a joint multi-omics analysis. The results revealed that compared to the C-L group, liver structural damage and stress were not significant in the environmental concentration group (T1-L) and the number of differential genes and differential metabolites were lower. However, as DBP stress concentration increased, the liver damage became severe, with significant vacuolation and hemolysis observed in the T2-L and T3-L groups. The TUNEL assay revealed a significant increase in the number of apoptotic cells along with a notable rise in differential genes and metabolites in the T2-L and T3-L groups. Oxidative stress markers (T-AOC, SOD, CAT, and GSH-PX) were also significantly higher in the T2-L and T3-L groups. RNA-Seq analysis showed that the protein processing in the endoplasmic reticulum pathway was most significantly -enriched differential gene pathway shared by both C-L vs T2-L and C-L vs T3-L, with most of the genes in this pathway showing significant up-regulation. This suggests that the protein processing in the endoplasmic reticulum pathway may play a key role in protecting the liver from injuries caused by high DBP stress. Interestingly, C XI, C XII, C XIII, C XIV and C XV in the chemical carcinogenesis - reactive oxygen species pathway were significantly down-regulated in the T2-L and T3-L groups based on combined transcriptomic and metabolomic analyses, suggesting that DBP causes liver injury by disrupting mitochondria. This comprehensive histomorphometric and multi-omics study demonstrated that the current DBP concentration in the habitat of <em>S. prenanti</em> in the upper reaches of the Yangtze River temporarily causes less liver damage. However, with increasing of DBP concentration, DBP could still cause serious liver damage to <em>S. prenanti</em>. This study provides a new mechanistic understanding of the liver response mechanism of <em>S. prenanti</em> under different concentrations of DBP stress and offers basic data for the ecological protection of the Yangtze River.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"285 ","pages":"Article 107390"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanisms of neurotoxic effects from combined exposure to polystyrene and microcystin-LR in Caenorhabditis elegans","authors":"Luyu Pei ,&nbsp;Lina Sheng ,&nbsp;Yongli Ye ,&nbsp;Jia-Sheng Wang ,&nbsp;Jian Ji ,&nbsp;Xiulan Sun","doi":"10.1016/j.aquatox.2025.107403","DOIUrl":"10.1016/j.aquatox.2025.107403","url":null,"abstract":"<div><div>Microplastics (MPs) are newly emerged pollutants found in water and soil, while microcystin-leucine arginine (MC-LR) is often detected in drinking water and water products, both posing serious threats to aquatic environment and food safety. MPs can serve as carriers of MC-LR. These pollutants are often found together, rather than separately. This study focused on assessing the neurotoxicity of co-exposure to MC-LR and PS in <em>Caenorhabditis elegans</em> (<em>C. elegans</em>) after combined exposure to these two pollutants. Exposure to varying concentrations of polystyrene (PS) and MC-LR individually caused a dose-dependent decrease in the locomotion behaviors of <em>C. elegans</em>. Exposure to either of these substances alone caused damage to the phenotypic indicators of the <em>C. elegans</em>. To further explore the additional damage caused by the combined exposure of PS and MC-LR, the low, medium, and high combined dose groups were selected based on the locomotion behaviors and survival results. Combined exposure increased the level of oxidative stress indicators and resulted in neuronal loss. It also reduced serotonin, glutamate, GABA, and dopamine neurotransmitters levels, without affecting cholinergic neurons. The expression of neurotransmitter-related genes also decreased. The high-dose group showed the most significant effects. This article is the first to study the combined effect of PS and MC-LR on <em>C. elegans</em> nervous systems, offering novel insights into the risks posed by co-occurring contaminants and their implications for aquatic ecosystems and food safety.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107403"},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}