Aquatic Toxicology最新文献

{"title":"Insights on nitrate pollution-induced intestinal dysfunction in turbot (Scophthalmus maximus) revealed by integrated dynamic metabolomics and transcriptomics","authors":"Jiachen Yu ,&nbsp;Suyue Zhou ,&nbsp;Ziyi Zhang ,&nbsp;Bo Qin ,&nbsp;Honglu Guo ,&nbsp;Anxin Shi ,&nbsp;Xiangyuan Li ,&nbsp;Xingqiang Wang ,&nbsp;Jie Lian ,&nbsp;Qing Ji","doi":"10.1016/j.aquatox.2025.107365","DOIUrl":"10.1016/j.aquatox.2025.107365","url":null,"abstract":"<div><div>Nitrate pollution in aquatic ecosystems has attracted global attention and has toxic effects on marine organisms. However, the precise molecular mechanisms underlying nitrate toxicity in the fish gut remain obscure. To this end, turbot were subjected to nitrate exposure (200 mg/L NO₃–N) for 0, 10, 20, and 30 days to explore nitrate toxicity and metabolic mechanisms in the gut by employing a multi-omics analysis integrating metabolomics with transcriptomics. The metabolomics analysis showed that nitrate exposure resulted in significant changes in the intestinal metabolite network, implying that the intestinal metabolism of turbot was impaired. Metabolites Pathway Analysis (MetPA) results revealed that the metabolic pathways significantly impacted by nitrate exposure included amino-acid metabolism pathways, such as phenylalanine, tyrosine, and tryptophan biosynthesis, phenylalanine metabolism, arginine biosynthesis, D-glutamine and D-glutamate metabolism, and aminoacyl-tRNA biosynthesis. Additionally, network interaction analysis between key differential metabolites (DMs) and differentially expressed genes (DEGs) identified seven essential amino acids associated with this process. Short Time-series Expression Miner (STEM) analysis determined that six distinct temporal expression patterns exhibited dynamic changes in DMs, mainly enriched in the metabolism of carbohydrates and lipids, indicating an increased energy demand to withstand nitrate stress. Multi-omics analysis revealed that sustained nitrate stress can interfere with protein digestion and absorption, alter collagen anabolism and specific composition of the extracellular matrix (ECM), and ultimately disrupt intestinal homeostasis. Our findings enhance our understanding of nitrate toxicity in fish and offer insights that can improve nitrate management in marine ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107365"},"PeriodicalIF":4.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of combined exposure to polyethylene and oxidized polycyclic aromatic hydrocarbons on growth, development, and neurobehavior in Zebrafish","authors":"Yu Liu ,&nbsp;Yujun Ning ,&nbsp;Yi Jiang ,&nbsp;Yiquan Ou ,&nbsp;Xiaobing Chen ,&nbsp;Chiting Zhong ,&nbsp;Ru Wang ,&nbsp;Zhibo Zhang ,&nbsp;Kongfan Wang ,&nbsp;Dingxin Long ,&nbsp;Weichao Zhao","doi":"10.1016/j.aquatox.2025.107361","DOIUrl":"10.1016/j.aquatox.2025.107361","url":null,"abstract":"<div><div>Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are a class of anthropogenic, persistent, and highly toxic PAH contaminants associated with developmental toxicity, 9-fluorenone (9-FLO) is a typical member of the OPAH family. Due to its ketone group, it has higher polarity, which results in increased solubility in water and greater potential for transport via atmospheric particles or water bodies. Polyethylene (PE), an amorphous polymer, is characterized by high diffusivity, high permeability, and a large internal molecular free volume, which confers a strong absorption capacity for organic pollutants. The effects of individual and combined exposures to these two common environmental pollutants on aquatic life remain unclear. In this study, we evaluated the effects of PE and 9-FLO exposure on growth, development, metabolism, and behavior using zebrafish as a model organism. We employed methods and techniques such as acridine orange staining, enzyme-linked immunosorbent assay (ELISA), video tracking, automated behavior analysis, microscopy imaging, and real-time fluorescence quantification. Zebrafish embryos at 2 h post-fertilization (hpf) were exposed to PE and 9-FLO, both individually and in combination. Our studies showed that exposure to PE or 9-FLO alone increases embryonic mortality and decreases hatchability compared to the control group. The 9-FLO group exhibited delayed hatching and inhibited larval length growth. The exposed groups showed a loose arrangement of telencephalic neurons, partial apoptosis, decreased dopamine (DA) content, increased serotonin (5-HT) content, decreased exercise capacity, reduced rhythmic amplitude, and increased rest time. The combined exposure group showed a slight alleviation of these effects compared to the single exposure groups but still exhibited significant differences from the control group. In summary, early exposure to PE and 9-FLO in zebrafish embryos, whether alone or in combination, affects growth, development, apoptosis, neurotransmitter release, and motor behavior of zebrafish neurons.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107361"},"PeriodicalIF":4.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multigenerational ecotoxicity of tris(2-butoxyethyl) phosphate to the tropical water flea Ceriodaphnia cornuta and probabilistic ecological risk assessment in freshwater environments","authors":"Tan-Duc Nguyen ,&nbsp;Van-Tai Nguyen ,&nbsp;Khuong V. Dinh ,&nbsp;Claudia Wiegand ,&nbsp;Zhen Wang ,&nbsp;Christine Baduel ,&nbsp;Thanh Luu Pham ,&nbsp;Thi Thuy Duong ,&nbsp;Quoc-Hung Nguyen ,&nbsp;Van Manh Do ,&nbsp;Thi Phuong Quynh Le ,&nbsp;Yu-Chen Huang ,&nbsp;Thanh-Son Dao","doi":"10.1016/j.aquatox.2025.107363","DOIUrl":"10.1016/j.aquatox.2025.107363","url":null,"abstract":"<div><div>The widespread use of the organophosphorus flame retardant tris(2-butoxyethyl) phosphate (TBOEP) and its presence in aquatic environments pose a hazard to wildlife. This study investigates the chronic ecotoxicity of TBOEP at environmentally relevant concentrations (6 ± 0.7 µg L⁻¹) in the tropical water flea <em>Ceriodaphnia cornuta</em> over seven generations. Delayed toxicity emerged from the second generation (F1) to the fourth generation (F3), affecting survival, body length, and fertility. Recovery occurred in F4, followed by complete inhibition in F5 and F6, where no organisms survived beyond day 10. Population dynamics revealed minimal growth in F5, leading to extinction by F6. This is the first study to demonstrate the multigenerational lethal effects of TBOEP at environmentally relevant concentrations, highlighting ecological threats to <em>C. cornuta</em> and other sensitive species. The mechanisms underlying these effects remain unclear and require sub-organismal investigation. The chronic predicted no-effect concentration, estimated at 8.64 µg L⁻¹, exceeds the tested concentration and therefore may not adequately protect vulnerable species. Ecological risk assessments based on TBOEP concentrations detected in surface waters since 2014 suggest potential risks at elevated levels in specific locations across multiple countries. Additional research is essential to validate TBOEP's toxicity across species under chronic and multigenerational exposure. Current ecological risk assessments likely underestimate TBOEP's threat to aquatic ecosystems, emphasizing the need for reassessment to better safeguard biodiversity.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107363"},"PeriodicalIF":4.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute toxicity, bioconcentration, and metabolomic profiling of agrochemical organosilicone spray adjuvants in Zebrafish","authors":"Tianyou Feng ,&nbsp;Jianling Liu ,&nbsp;Ling Wu ,&nbsp;Yuanqiang Luo ,&nbsp;Ling Liu ,&nbsp;Fen Li ,&nbsp;Guangbin Wu ,&nbsp;Ping Lu ,&nbsp;Yuping Zhang","doi":"10.1016/j.aquatox.2025.107362","DOIUrl":"10.1016/j.aquatox.2025.107362","url":null,"abstract":"<div><div>Agrochemical organosilicone spray adjuvants, widely applied in agroecosystems, are suspected to pose environmental risks based on recent studies. In response to these concerns, we have purified the active components of these adjuvants, specifically a series of oligomers of hydroxy(polyethyleneoxy) propyl-heptamethyl trisiloxane (TSS-H), including ethylene oxide (EO) units ranging from 4 to 12. For analysis of TSS-H in zebrafish and water, a method utilizing high-performance liquid chromatography coupled with high resolution mass spectrometry (HPLC<img>HRMS) was developed. The findings revealed that at total TSS-H concentrations of 0.04, 0.4, and 4 mg/kg, the average recovery rate of TSS-H containing 4 to 12 EO units in zebrafish ranged from 81.2 % to 107.2 %, with relative standard deviations (RSDs) between 0.1 % and 8.4 %. The 96-hour LC₅₀ of TSS-H in zebrafish was 6.71±0.04 mg/L. At exposure concentrations of 0.0671 mg/L and 0.671 mg/L, TSS-H oligomers reached a steady state in zebrafish within 14–21 days, with concentrations ranging from 0.008 to 0.05 mg/kg and 0.018 to 0.348 mg/kg, respectively. Bioconcentration factor (BCF) values for TSS-H oligomers (4–12 EO units) were 1.87–11.59 at the low dose and 0.79–9.19 at the high dose after 14 days. The elimination half-lives of the oligomers were &lt;2.37 days during the elimination phase. Non-targeted metabolomic analysis revealed both TSS-H doses significantly impacted lipid and amino acid metabolic pathways, with disruptions largely associated with abnormal energy metabolism, oxidative stress, and alterations in membrane composition. These findings are critical for assessing the food safety and environmental risks of agrochemical organosilicone spray adjuvants in fish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107362"},"PeriodicalIF":4.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluoxetine impairs gamete function and fertilization success in Tegillarca granosa: environmental risks of antidepressant contamination","authors":"Yu Han ,&nbsp;Xingzhou Yu ,&nbsp;Guanghui Wang ,&nbsp;Shanjie Zha ,&nbsp;Wei Shi ,&nbsp;Zhiquan Liu ,&nbsp;Hangjun Zhang ,&nbsp;Guangxu Liu","doi":"10.1016/j.aquatox.2025.107364","DOIUrl":"10.1016/j.aquatox.2025.107364","url":null,"abstract":"<div><div>In recent years, the antidepressant fluoxetine (FLX) has been increasingly detected in global environments, emerging as a contaminant with significant toxic effects. However, its impact on the fertilization processes of broadcast-spawning species remains unclear. This study focuses on <em>Tegillarca granosa</em>, a broadcast-spawning bivalve, to evaluate the effects of fluoxetine on gametes and fertilization success. The findings revealed that FLX significantly reduced sperm motility, including curvilinear velocity, average path velocity, and straight-line velocity. Further analysis demonstrated that FLX impaired sperm motility by inhibiting ATP production and reducing cellular activity. Additionally, FLX altered Ca²⁺ homeostasis and caspase activity in both sperm and eggs, and suppressed mitochondrial energy supply in eggs. By assessing gamete collision probabilities and fusion rates, the study systematically confirms the considerable fertilization toxicity of FLX in <em>T. granosa</em>. These findings provide critical insights into the environmental risks posed by FLX contamination.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107364"},"PeriodicalIF":4.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of polystyrene nanoplastics on the intestinal histopathology, oxidative stress, and microbiota of Acrossocheilus yunnanensis","authors":"Zhaolai Guo ,&nbsp;Ruisi Yang ,&nbsp;Zexiang Hua ,&nbsp;Wenyu Long ,&nbsp;Qianqian Xiang","doi":"10.1016/j.aquatox.2025.107359","DOIUrl":"10.1016/j.aquatox.2025.107359","url":null,"abstract":"<div><div>Even though extensive research exists on the negative impact of nanoplastics on fish, their effect on the microbiota and intestinal health of freshwater fish remains unclear. This study investigated the impact of polystyrene nanoplastics (PS-NPs) on the microbiota, oxidative stress, and intestinal morphology of the <em>Acrossocheilus yunnanensis</em> (<em>A. yunnanensis</em>) freshwater fish species. The findings demonstrated that PS-NPs induced structural changes (e.g., epithelial rupture and microvilli damage) in the intestinal tissue of <em>A. yunnanensis</em>. Meanwhile, they increased the level of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in the intestine, but did not significantly cause changes in the activities of catalase (CAT) and glutathione S-transferase (GST) enzymes. The microbiome results indicated that PS-NPs increased gut microbial community diversity and Proteobacteria abundance while decreasing the Fusobacteriota content. Furthermore, PS-NPs significantly improved multiple microbial functions such as amino acid and lipid transfer and metabolism, as well as energy generation and conversion. Overall, this study revealed that PS-NPs caused oxidative stress and microbiota dysbiosis in <em>A. yunnanensis</em>, possibly causing intestinal epithelial damage. This research elucidates the mechanism underlying PS-NP toxicity to freshwater fish and its subsequent impact.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107359"},"PeriodicalIF":4.1,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual impacts of elevated pCO2 on the ecological effects induced by microplastics and nanoplastics: A study with Chlamydomonas reinhardtii","authors":"Chengwei Liang ,&nbsp;Yajing Liu ,&nbsp;Tianle Xi ,&nbsp;Jia Liu ,&nbsp;Shanshan Ge ,&nbsp;Xiaowen Zhang ,&nbsp;Zhihua Jia ,&nbsp;Naihao Ye","doi":"10.1016/j.aquatox.2025.107358","DOIUrl":"10.1016/j.aquatox.2025.107358","url":null,"abstract":"<div><div>Aquatic organisms face increased complexity and severity when exposed to the combined stressors of climate change and micro- and nanoplastics (MNPs), as opposed to facing these stressors individually. This study examined the effects and underlying mechanisms of elevated <em>p</em>CO₂, which leads to freshwater acidification, as well as amino-modified polystyrene MNPs (PS-NH₂ MNPs) of varying sizes (5 μm, 300 nm, 80 nm), on <em>Chlamydomonas reinhardtii</em> under both individual and combined conditions. The results showed a size-dependent toxicity of PS MNPs, with the smaller nanoparticles (80 nm) causing greater toxic inhibition than the larger microparticles (5 μm and 300 nm), primarily attributed to oxidative stress-related cellular damage. In contrast, freshwater acidification (FA) appeared to promote the growth of <em>C. reinhardtii</em>, possibly by upregulating transcripts associated with energy metabolism. However, when <em>C. reinhardtii</em> was exposed to both FA and MNPs simultaneously, distinct toxic effects were observed. The co-exposure to FA and NPs induced the most severe oxidative stress, implying the greatest energetic cost. This stress resulted in the downregulation of pathways involved in fatty acid biosynthesis and protein folding, ultimately causing significant damage to cellular structure and function. The increased energy from the upregulation of the TCA cycle was mainly allocated for DNA damage repair and cell division, which induced an energy deficit necessary for stress resistance. In contrast, during co-exposure to FA and MPs, energy was redirected towards DNA replication and the synthesis of anti-stress substances, facilitating recovery and promoting growth. Our study highlighted the decisive influence of climate change and particle size in assessing the ecological effects and risks associated with MNPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107358"},"PeriodicalIF":4.1,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The sensitivity of embryos and larvae of the crown-of-thorns sea star to copper and zinc: with respect to conditions experienced on the Great Barrier Reef and water quality guidelines","authors":"Regina Balogh,&nbsp;Maria Byrne","doi":"10.1016/j.aquatox.2025.107357","DOIUrl":"10.1016/j.aquatox.2025.107357","url":null,"abstract":"<div><div>Our understanding of the ecotoxicology for tropical marine species is limited. We investigated the impacts of copper (0.1–6 µg/L) and zinc (2.5–45 µg/L) on development of the crown-of-thorns sea star (COTS) (<em>Acanthaster</em> sp.) with respect to lethal concentration (LC) and effective concentration (EC) for inhibition of larval swimming and in context with concentrations reported for the Great Barrier Reef (GBR) and the Australia and New Zealand Water Quality Guidelines (WQG). For embryos, copper caused 100 % mortality by 24 h and zinc arrested development by 36 h. The LC50s for bipinnaria for copper at 24 and 48 h were 0.67 and 0.54 µg/L and for zinc were 52.36 and 27.01 µg/L, respectively. For brachiolaria, these were 2.82 and 0.85 µg/L and 28.89 and 22 µg/L, respectively. For larval swimming the EC50 for copper was 0.35 µg/L and 0.66 µg/L in bipinnaria and brachiolaria, respectively. Larvae placed in copper (0.1–0.2 µg/L) recovered their swimming ability. Bipinnaria were resistant to zinc, EC50 of 28.89 µg/L for swimming, while for brachiolaria this was 7.18 µg/L. <em>Acanthaster</em> development was sensitive to copper and zinc at levels reported for nearshore GBR waters. This may contribute to the general absence of COTS on inshore reefs. Our data showed that the 95 % species protection for copper and zinc (1.3 µg/L and 15 µg/L, respectively) are not suitable for COTS development. This is likely to apply to many other species with similar planktonic larvae in GBR waters. This study will contribute to development of WQG for tropical marine waters.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107357"},"PeriodicalIF":4.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological impacts of agrochemical and pharmaceutical antifungals on a non-target aquatic host-parasite model","authors":"AR Pimentão ,&nbsp;R. Ribeiro ,&nbsp;BA Silva ,&nbsp;AP Cuco ,&nbsp;BB Castro","doi":"10.1016/j.aquatox.2025.107356","DOIUrl":"10.1016/j.aquatox.2025.107356","url":null,"abstract":"<div><div>Agrochemical fungicides and their pharmaceutical counterparts are a major anthropogenic threat to the biodiversity of freshwater ecosystems as they affect non-target organisms (including aquatic fungi) and disrupt the processes in which they intervene. The goal of this work was to assess the effects of four common agricultural fungicides differing in their modes of action (azoxystrobin, carbendazim, folpet, and mancozeb) and an antifungal pharmaceutical (clotrimazole) on a host × parasite experimental model. We conducted 21-day life history experiments with <em>Daphnia magna</em> (the host) in the absence or presence of <em>Metschnikowia bicuspidata</em> (a microparasitic yeast) to evaluate the effect of each fungicide on the outcome of this relationship (disease) and the fitness of both host and parasite. Interactive but context-dependent effects were observed in <em>D. magna</em> life history responses upon concomitant exposure to parasite and toxicant. The parasite had a drastic negative effect on host survival and reproduction. Carbendazim, clotrimazole and folpet significantly decreased host fitness. In some cases (depending on the combination of toxicant and measured endpoint), simultaneous exposure of the host to the parasite and fungicides led to a slight decrease in host reproduction, which was absent when only the fungicide was present. In two other cases, the fungicide interfered with the host-parasite relationship: azoxystrobin had an impact on infection intensity (decreasing spore load per host), whereas clotrimazole demonstrated a strong antiparasitic effect, clearing all signs of infection (0 % prevalence). These findings emphasize the context-dependent nature of the interaction between pollution and disease.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"284 ","pages":"Article 107356"},"PeriodicalIF":4.1,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic disruptions induced by low concentrations of DMSO in RTgill-W1 fish cells: The importance of solvent controls in in vitro studies","authors":"Thao V. Nguyen ,&nbsp;Ali Alfarsi ,&nbsp;Huong Thanh Nguyen ,&nbsp;Georgia Davidson ,&nbsp;Natoiya D.R. Lloyd ,&nbsp;Anu Kumar","doi":"10.1016/j.aquatox.2025.107354","DOIUrl":"10.1016/j.aquatox.2025.107354","url":null,"abstract":"<div><div>Dimethyl sulfoxide (DMSO) is a widely used solvent in biological research due to its ability to enhance membrane permeability, facilitating drug delivery and molecular transport across cellular membranes. However, its effects on cellular metabolism, especially at low concentrations, remain insufficiently understood. This study investigated the metabolic disruptions induced by 0.1–10 % DMSO in the RTgill-W1 fish cell line, focusing on changes in cell viability, oxidative stress, and key metabolic pathways. Results revealed that DMSO exposure caused dose-dependent declines in cell viability at 0.5 % DMSO and increases in reactive oxygen species (ROS) at 4 % and higher, indicating elevated oxidative stress. Metabolomic profiling revealed altered levels of numerous metabolites and significant impacts on 41 metabolic pathways belonging to five major functional groups: amino acid metabolism, carbohydrate metabolism, lipid metabolism, vitamin and co-factor metabolism, and nucleotide metabolism. The effects were observed across all exposure concentrations (0.1, 0.5, 1, 4, and 8 %), with more pronounced impacts at higher concentrations. These findings highlight that DMSO, even at low concentrations (≤ 0.5 %), can have widespread effects on cellular metabolism, impacting experimental outcomes in <em>in vitro</em> studies. This study provides valuable insights into the biochemical impacts of DMSO on fish cell lines and emphasizes a caution in using DMSO in biological research to minimize unintended cellular effects. Additionally, it highlights the critical need to include solvent controls at matching concentrations to accurately distinguish solvent-induced effects from those caused by experimental treatments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107354"},"PeriodicalIF":4.1,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}