Aquatic Toxicology最新文献

{"title":"Combined toxicity of microplastic fibers and dibutyl phthalate on algae: Synergistic or antagonistic?","authors":"Le Liang ,&nbsp;Yangyang Liang ,&nbsp;Min Su ,&nbsp;Zhe Wang ,&nbsp;Zhendong Zhou ,&nbsp;Xiaotao Zhou ,&nbsp;Zhongguan Jiang","doi":"10.1016/j.aquatox.2025.107290","DOIUrl":"10.1016/j.aquatox.2025.107290","url":null,"abstract":"<div><div>Plastics, combined with plasticizers, have been widely utilized worldwide. Microplastic fibers (MPFs) and dibutyl phthalate (DBP) account for the most predominant microplastics and plasticizers detected in freshwater ecosystem, with their joint toxicity being limited studied. In this study, we employed freshwater algae (<em>Chlorella vulgaris</em>) as toxicity test model organism to assess their growth, photosynthesis, metabolism, and oxidative response when exposing to different concentrations of polypropylene MPFs and the co-exposure of DBP. In addition, the toxic interaction between MPFs and DBP was assessed by combining the integrated toxicity value (Integrated Biomarker Response version 2, IBRv2) and the mixture toxicity index (Effect Addition Index, EAI). Our results demonstrated significant toxic effects of MPFs and DBP on <em>C. vulgaris</em>, and highlighted their dynamic interactions with <em>C. vulgaris</em>. Specifically, when combining with DBP, MPFs with high concentrations exhibited significantly increase in algae growth inhibition, photosynthetic pigment contents (Chl-a, Chl-b, and carotenoids), protein contents, and oxidative enzymes (SOD, CAT, and MDA). In terms of integrated toxicity values, higher IBRv2 values were recorded by the combined exposure of MPFs and DBP in contrast with the sole exposure groups, indicating that the combined exposure caused more severe damage to photosynthesis, oxidation and metabolism. In addition, our study recorded synergistic combined toxicity when MPFs were in high concentrations, whereas antagonistic combined toxicity when MPFs were in low concentrations. Our study highlights the MPFs concentration-dependent combined toxicity (synergistic or antagonistic) when exposing to microplastics and plasticizers in freshwater ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"281 ","pages":"Article 107290"},"PeriodicalIF":4.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to complex mixtures of urban sediments containing Tyre and Road Wear Particles (TRWPs) increases the germ-line mutation rate in Chironomus riparius","authors":"Lorenzo Rigano ,&nbsp;Markus Schmitz ,&nbsp;Volker Linnemann ,&nbsp;Martin Krauss ,&nbsp;Henner Hollert ,&nbsp;Markus Pfenninger","doi":"10.1016/j.aquatox.2025.107292","DOIUrl":"10.1016/j.aquatox.2025.107292","url":null,"abstract":"<div><div>Tyre and road wear particles (TRWPs) are a significant yet often underestimated source of environmental pollution, contributing to the accumulation of microplastics and a complex mixture of contaminants in both terrestrial and aquatic ecosystems. Despite their prevalence, the long-term evolutionary effects of TRWPs, beyond their immediate toxicity, remain largely unknown. In this study, we assessed mutagenicity in the non-biting midge <em>Chironomus riparius</em>, upon exposure to urban sediment collected from a runoff sedimentation basin. To assess the extent of mutagenic effects over multiple generations, we combined the urban sediment exposure model with short-term mutation accumulation lines (MALs) and subsequent whole genome sequencing (WGS). The study was conducted over five generations, with urban sediment concentrations of 0.5 % and 10 %. Our results reveal that the exposure to urban sediment significantly increases mutation rates compared to control groups by 50 %, independent of concentration (0.5 % and 10 %). To infer potential causal processes, we conducted a comparative analysis using known mutational spectra from previous studies. This comparison showed that the mutation profiles induced by urban sediment clearly clustered with those caused by Benzo[a]Pyrene (BaP), a known Polycyclic Aromatic Hydrocarbon (PAH). A comprehensive chemical characterization of the sediment confirmed a considerable impact of road runoff and traffic-related contamination, including PAHs of primarily petrogenic origin. This suggests that PAH-like compounds present in urban sediments may play a significant role in the observed mutagenic effects. Our study shows that urban sediments influence mutation rates and alter mutational spectra in exposed organisms, potentially compromising genomic stability and shaping evolutionary trajectories. These genetic changes can have profound long-term effects on population dynamics and ecosystem health, underlining the importance of understanding the evolutionary consequences of environmental pollution. Additionally, we show that comparatively analysing of mutational spectra may provide valuable insights into mutational processes.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"281 ","pages":"Article 107292"},"PeriodicalIF":4.1,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute cardiorespiratory effects of 6PPD-quinone on juvenile rainbow trout (Oncorhynchus mykiss) and arctic char (Salvelinus alpinus)","authors":"Summer J. Selinger ,&nbsp;David Montgomery ,&nbsp;Steve Wiseman ,&nbsp;Markus Hecker ,&nbsp;Lynn Weber ,&nbsp;Markus Brinkmann ,&nbsp;David Janz","doi":"10.1016/j.aquatox.2025.107288","DOIUrl":"10.1016/j.aquatox.2025.107288","url":null,"abstract":"<div><div>N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine-quinone (6PPD-quinone) is an environmental transformation product of the widely used rubber tire antioxidant, 6PPD. Found in stormwater runoff, 6PPD-quinone has been reported to cause acute lethality at ≤1 μg/L in salmonids like coho salmon, rainbow trout, and brook trout. Conversely, other species such as Arctic char and brown trout are insensitive, even when exposed to significantly greater concentrations (3.8–50 μg/L). Sensitive species exhibit symptoms such as gasping, spiraling, increased ventilation, and loss of equilibrium, suggesting a possible impact on cardiorespiratory physiology. This study investigated sublethal 6PPD-quinone toxicities, focusing on cardiovascular and metabolic effects in two salmonids of varying sensitivity: a sensitive species, rainbow trout (<em>Oncorhynchus mykiss)</em> and a tolerant species, Arctic char (<em>Salvelinus alpinus</em>). Fish were exposed to measured concentrations of 0.59 or 7.15 μg/L 6PPD-quinone, respectively, in respirometry chambers for 48 h to assess temporal changes in resting oxygen consumption compared to unexposed controls. Following exposure, cardiac ultrasound and electrocardiography characterized cardiac function <em>in vivo,</em> while blood gas analysis examined blood composition changes. In both species, changes in resting oxygen consumption were observed. In rainbow trout only, a decrease in end systolic volume and an increase in passive ventricular filling, cardiac output, and PR interval length were observed, indicating cardiac stimulation. Cardiorespiratory symptoms observed following rainbow trout exposure might partly be driven by a significant increase in methemoglobin, resulting in an impaired ability to oxygenate tissues. This study is the first to examine the effects of 6PPD-quinone exposure on the cardiorespiratory system of salmonid fishes and provides information invaluable to a better understanding of the mechanism of 6PPD-quinone toxicity.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107288"},"PeriodicalIF":4.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling the size distribution and bioaccumulation of gold nanoparticles under mixture exposure","authors":"Yao Li ,&nbsp;Xiangrui Wang ,&nbsp;Dingyuan Liang ,&nbsp;Xiaoli Zhao ,&nbsp;Zhaomin Dong ,&nbsp;Yingchen Bai ,&nbsp;Wen-Xiong Wang ,&nbsp;Willie J.G.M. Peijnenburg ,&nbsp;Ying Wang ,&nbsp;Wenhong Fan","doi":"10.1016/j.aquatox.2025.107286","DOIUrl":"10.1016/j.aquatox.2025.107286","url":null,"abstract":"<div><div>To assess the bioaccumulation and toxicity of nanoparticles (NPs), analyzing and modelling the relationship between the size distribution of NPs in organisms and the exposure particle size distribution represents an important challenge. Previous studies mostly focused on the NPs with single size. However, the size distribution of NPs is wide and variable in the natural environment. There is a lack of research on the NPs with mixed sizes. This study investigated the size distribution of three gold (Au) NPs with different sizes and their mixtures within a ciliate <em>Tetrahymena thermophila</em> under the same number concentration of particles. Results revealed that smaller particles tended to aggregate and bioaccumulate more in cells. Using expectation–maximization algorithm, a particle size distribution model of NPs in cells was established. This model effectively simulated the size distribution of NPs with mixed sizes in cells, demonstrating high accuracy with a mean absolute error of &lt; 0.001, a root mean squared error of &lt; 0.001, and a correlation coefficient exceeding 0.98. Experimental results further verified that the model reliably predicted the size distribution of NPs with mixed sizes in cells, and smaller particles accounted for a larger proportion of the size distribution and bioaccumulation. These results demonstrated the importance of particle size and size distribution of NPs in their environmental effects. Models developed here can provide guidance for future evaluation of the environmental risks of NPs mixtures.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107286"},"PeriodicalIF":4.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIF-induced ROS-mediated cytotoxicity and genotoxicity in embryonic cell culture of Daphnia magna","authors":"Sreevidya CP ,&nbsp;Ajitha V ,&nbsp;Manoj Kumar TM ,&nbsp;Manomi S ,&nbsp;Bhavya K ,&nbsp;I․S․Bright Singh ,&nbsp;Jayesh Puthumana","doi":"10.1016/j.aquatox.2025.107285","DOIUrl":"10.1016/j.aquatox.2025.107285","url":null,"abstract":"<div><div>Bifenthrin (BIF) is a widely used synthetic pyrethroid insecticide that poses significant risks to the environment, particularly to aquatic ecosystems. In the present study, the cytotoxic and genotoxic effects of BIF on <em>Daphnia magna</em> cells were evaluated using <em>in vitro</em> methods. To achieve this, we developed a novel embryonic cell culture system from <em>D.magna</em> using Modified Schneider's Insect Medium (MSIM), which demonstrated remarkable viability for over two months. The lethal concentration 50 (LC₅₀) values of BIF were determined using this cell culture system through XTT (2,3-bis-(2‑methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide)assays, yielding values of 7.4 µg/mL and 4.3 µg/mL for 24 h and 48 h exposures, respectively. A fluorometric intracellular reactive oxygen species (ROS) assay was employed to measure ROS production, revealing that BIF exposure induced oxidative stress in a dose-dependent manner. The activities of Glutathione peroxidase (GPx), glutathione (GSH), and glutathione-S-transferase (GST) were significantly reduced, indicating oxidative damage. Co-treatment with N-acetylcysteine(NAC) mitigated these effects, restoring antioxidant enzyme activity and reducing (ROS) levels. Gene expression analysis via quantitative real-time PCR (qPCR) showed upregulation of stress-related genes (hsp70, hsp90) and antioxidant genes (Mn/ZnSod, cat) following exposure to LC₅₀ concentrations of BIF. However, prolonged exposure led to a downregulation of these genes, suggesting cumulative effects over time. The comet assay confirmed that BIF caused genotoxicity, as evidenced by significant increases in comet and tail lengths. Co-treatment with NAC effectively mitigated these genotoxic effects. This study highlighted the cytotoxic and genotoxic potential of BIF in aquatic organisms and suggested the need for environmentally friendly pest control strategies. Also, the findings confirmed the reliability of <em>D. magna</em> embryonic cell cultures for assessing the toxicological effects of environmental pollutants, offering new possibilities for <em>in vitro</em> toxicity testing at cellular and molecular levels.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107285"},"PeriodicalIF":4.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicological effects and bioconcentration of a dissolved Organophosphate ester's mixture in the marine flagellate Isochrysis galbana","authors":"Hela Ben Gharbia ,&nbsp;Brigid Carr ,&nbsp;Jean Luc Rolland ,&nbsp;Philippe Bersuder ,&nbsp;Marc Metian ,&nbsp;Imma Tolosa","doi":"10.1016/j.aquatox.2025.107283","DOIUrl":"10.1016/j.aquatox.2025.107283","url":null,"abstract":"<div><div>Organophosphate esters (OPEs) are emerging organic contaminants due to their widespread use, environmental persistence and bioaccumulation potential. They are released into the environment and may affect the physiology of various marine organisms. To evaluate the effects of OPEs on marine microalgae, the phytoplankton species <em>Isochrysis galbana</em> was exposed to a mixture of 11 OPEs, and their impacts on growth, reactive oxygen species (ROS) production, lipid content, and their bioconcentration in cells were assessed. Results showed that after 11 days of exposure, growth was significantly inhibited (<em>p</em> &lt; 0.05) at elevated OPE concentrations (5 and 10 µg <span>l</span>^-1 of each OPE). For 10 µg <span>l</span>^-1 of each OPE, cell densities decreased by 76 % and growth rates were 23 % below those measured in the control. A stimulation of ROS production was observed even at environmentally relevant OPE concentrations (0.5 µg <span>l</span>^-1 for each OPE), and the increase reached up to 3.6 times the ROS production of the control (<em>p</em> &lt; 0.05) after 8 days of exposure to the highest tested concentration (10 µg <span>l</span>^-1 of each OPE). Moreover, a positive correlation (r² = 0.85, <em>p</em> &lt; 0.05) was observed between bioconcentration factor (BCF) and log K_ow. Interestingly, 3 out of the 11 OPEs: ethylhexyldiphenyl phosphate -EHDP-, tris(2-ethylhexyl) phosphate -TEHP-, and tritolyl phosphate -TMPP-, exceeded the BCF threshold values of 2000 L kg^-1, considered to be bioacumulative in aquatic species according to European Union legislation. Together our results suggest that (1) OPEs affect <em>I. galbana</em> cells, mainly at high concentrations but to a certain extend at environmentally relevant levels, and (2) This species can bioconcentrate OPEs and represents a potential pathway through which these contaminants enter marine food webs. This study provides the first assessment of OPE accumulation in a microalgae frequently used in aquaculture.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"281 ","pages":"Article 107283"},"PeriodicalIF":4.1,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterials exert toxic effects on aquatic organisms by inhibiting respiration, inducing oxidative stress, mitochondrial dysfunction and autophagy","authors":"Hagar Fadda ,&nbsp;Raheema Hassan Khan ,&nbsp;Yara Shqair ,&nbsp;Cemile Uslu ,&nbsp;Alexander V. Panov ,&nbsp;Alex Lyakhovich","doi":"10.1016/j.aquatox.2025.107284","DOIUrl":"10.1016/j.aquatox.2025.107284","url":null,"abstract":"<div><div>Although bactericidal antibiotics are an integral part of infectious disease medicine, the uncontrolled use of these drugs in recent years is beginning to affect the environment, especially water resources. With the exception of a few well-documented toxic effects, antimicrobials are thought to act mainly on bacteria and, at low doses, have no deleterious effects on eukaryotic organisms. However, since mitochondria are direct ancient relatives of prokaryotes, mitochondrial dysfunction induced by antibiotic exposure can also occur in higher eukaryotes. In this work, we used the microcrustacean <em>Artemia salina</em> to show how some known antibacterial drugs exert toxic effects on mitochondria, suppressing cellular and organismal respiration in aquatic organisms. We have demonstrated that exposure to antimicrobials of different classes, namely kanamycin A, tetracycline hydrochloride, erythromycin, and chloramphenicol, leads to the accumulation of reactive oxygen species and is accompanied by autophagy. Our results suggest that the emergence of antibactericidal drugs, increasingly appearing in wastewater, may have far-reaching consequences for aquatic areas adjacent to large cities.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107284"},"PeriodicalIF":4.1,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticidal nanoformulations based on a lipid matrix: Physicochemical properties and their effect on non-target aquatic biota","authors":"Catarina Ganilho ,&nbsp;Manuel A. Andino-Enríquez ,&nbsp;Thacilla I. de Menezes ,&nbsp;Lisete Fernandes ,&nbsp;Carlos M. Pereira ,&nbsp;Ruth Pereira ,&nbsp;Tatiana Andreani","doi":"10.1016/j.aquatox.2025.107268","DOIUrl":"10.1016/j.aquatox.2025.107268","url":null,"abstract":"<div><div>The incorporation of lambda-cyhalothrin (LC) in lipid nanoparticles (LN) could be a sustainable strategy to increase its efficacy and decrease its hazard to the environment. The purpose of the present work was to perform the interaction between LC and LN after nanoencapsulation and to evaluate their effect on species from different aquatic trophic levels such as <em>Aliivibrio fischeri, Raphidocelis subcapitata, Lemna minor,</em> and <em>Daphnia magna</em>. LN loaded with LC (LN-LC) were produced by green and simple methodology without organic solvents using Precirol ATO5® and Capryol 90® as solid and liquid lipids, respectively, and soy lecithin and TEGO® Care as emulsifiers. The physicochemical interaction between LC and LN was assessed by differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR), and X-ray, confirming that LC is associated with the lipid lattice of nanoparticles, characterized by an amorphous matrix. The data from biological tests showed no or low toxicity of LN-LC on the selected aquatic organisms. Thus, encapsulation in lipid-based nanoparticles may be a promising and sustainable choice for using this insecticide in agricultural practices, reducing its environmental risk.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107268"},"PeriodicalIF":4.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effect of mercury and ammonia toxicity and its mitigation through selenium nanoparticles in fish","authors":"Anupama Shinde ,&nbsp;Rajesh Sharma ,&nbsp;Prem Kumar ,&nbsp;Tarkeshwar Kumar ,&nbsp;Kotha Sammi Reddy ,&nbsp;Neeraj Kumar","doi":"10.1016/j.aquatox.2025.107270","DOIUrl":"10.1016/j.aquatox.2025.107270","url":null,"abstract":"<div><div>An experiment was conducted to mitigate mercury and ammonia toxicity (Hg + NH₃) in <em>Oreochromis niloticus</em> (GIFT strain) using selenium nanoparticles (Se-NPs). The Se-NPs were synthesized using green methods, employing fish waste as the substrate. Experimental diets were prepared by supplementing Se-NPs at 0, 0.3, and 0.6 mg kg⁻¹. The oxidative stress enzymes, including catalase (CAT) and glutathione peroxidase (GPx), in the liver and kidney tissues were significantly reduced by Se-NPs at 0.3 and 0.6 mg kg⁻¹ under Hg + NH₃ stress compared to the control and stressor in 20 and 40 day periods. Additionally, superoxide dismutase (SOD) activity in the kidney at 20 days and in the liver at 40 days was significantly reduced by supplementation of Se-NPs under similar conditions. The activity of acetylcholine esterase (AChE), was significantly inhibited by Hg + NH₃ toxicity. Whereas, AChE activity was enhanced by Se-NPs supplementation at 0.3 and 0.6 mg kg⁻¹ during 20 and 40 day intervals. The gene expression of <em>HSP70, iNOS, CYP450, Caspase-3a</em>, and <em>TNFα</em> in liver tissue, and <em>MYST</em> in muscle tissue was upregulated by Hg+NH₃ toxicity. However, this upregulation was significantly downregulated by supplementation of Se-NPs at 0.3 and 0.6 mg kg⁻¹ under Hg + NH₃ stress. Moreover, immunoglobulin (<em>Ig</em>) and growth hormone (<em>GH)</em> levels were noticeably upregulated with Se-NPs compared to the control and Hg+NH₃ stress. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) in liver and gill tissues, were significantly elevated by Hg+NH₃ toxicity, were reduced by Se-NPs diet. Conversely, digestive enzyme activities, including protease, amylase, and lipase, were significantly enhanced by Se-NPs under stress conditions. Dietary supplementation with Se-NPs at 0.3 and 0.6 mg kg⁻¹ improved growth performance parameters such as final weight gain percentage, feed conversion ratio, protein conversion ratio, specific growth rate, daily growth index, and relative feed intake compared to the control and other groups. DNA damage, assessed in terms of tail DNA percentage, was significantly reduced with Se-NPs supplementation. Additionally, mercury detoxification was significantly enhanced with Se-NPs-supplemented diets. In conclusion, this study demonstrates that dietary Se-NPs effectively alleviate the adverse effects of mercury and ammonia toxicity by modulating antioxidant status, enhancing immunomodulation, and mitigating stress biomarker impacts through changes in gene expression in fish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107270"},"PeriodicalIF":4.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolonged curcumin supplementation causes tissue-specific antioxidant responses in adult oysters: Potential implications for resilience against abiotic and biotic stressors in the aquaculture industry","authors":"Heloísa Bárbara Gabe ,&nbsp;Karine Amabile Taruhn ,&nbsp;Danielle Ferraz Mello ,&nbsp;Melody Lebrun ,&nbsp;Christine Paillard ,&nbsp;Charlotte Corporeau ,&nbsp;Alcir Luiz Dafre ,&nbsp;Rafael Trevisan","doi":"10.1016/j.aquatox.2025.107282","DOIUrl":"10.1016/j.aquatox.2025.107282","url":null,"abstract":"<div><div>Aquatic animals inhabiting marine coastal environments are highly susceptible to environmental fluctuations and pollution, exemplified by widespread mass mortalities induced by marine bacteria or viruses. Enhancing antioxidant defenses presents a promising strategy to mitigate such environmental stressors. We postulated that supplementation of oysters with natural compounds such as flavonoids, exemplified by curcumin (CUR), could effectively bolster their antioxidant protection. Adult Pacific oysters were supplemented with CUR (30 μM) in seawater for 2, 4, 8, and 16 days. CUR metabolites progressively accumulated in gills, mantle, and digestive glands. Notably, oyster antioxidant response was significantly augmented, as evidenced by elevated glutathione (GSH) levels, and enhanced activities of glutathione reductase (GR), thioredoxin reductase (TrxR), and glutathione S-transferase (GST) after 4, 8, and 16 days of CUR supplementation. This response was tissue-specific, with the most pronounced increase in gills, followed by mantle, whereas digestive gland exhibited minimal response. After being supplemented with CUR for 8 days, oysters were subjected to antioxidant-disrupting agents such as N-ethylmaleimide (NEM), 1‑chloro-2,4-dinitrobenzene (CDNB). Both chemicals reduced antioxidant protection in untreated animals. However, CUR supplementation prevented these redox-disrupting effects, suggesting the potential ability of CUR to counteract antioxidant stressors. The effects of 8 days of CUR supplementation were also tested against the lethal effects of the pathogens V<em>. tapetis,</em> V<em>, alginolyticus,</em> and V<em>. anguillarum,</em> but CUR failed to induce immunological protection<em>.</em> The antioxidant protection induced by CUR holds promise for application in aquaculture to bolster animal health and resilience against abiotic stressors. Further research is needed to investigate the long-term impact of CUR supplementation and its role against biotic stressors, such as bacterial and viral infections.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"280 ","pages":"Article 107282"},"PeriodicalIF":4.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}