Aquatic Toxicology最新文献

{"title":"Genes of filter-feeding species as a potential toolkit for monitoring microplastic impacts","authors":"Marta Pilar Ortiz-Moriano,&nbsp;Eva Garcia-Vazquez,&nbsp;Gonzalo Machado-Schiaffino","doi":"10.1016/j.aquatox.2024.107234","DOIUrl":"10.1016/j.aquatox.2024.107234","url":null,"abstract":"<div><div>Microplastics (MPs) are ubiquitous in the marine environment and impact organisms at multiple levels. Understanding their actual effects on wild populations is urgently needed. This study develops a toolkit to monitor changes in gene expression induced by MPs in natural environments, focusing on filter-feeding and bioindicator species from diverse ecological and taxonomic groups. Six candidate genes —Caspase, HSP70, HSP90, PK, SOD, and VTG— and nine filter-feeding species —two branchiopods, one copepod, five bivalves and one fish— were selected based on differential expression in response to MPs exposure (mainly the widely used polystyrene and polyethylene polymers) reported in over 30 publications. Some genes are particularly determinant, such as HSP70 and HSP90 (key to managing a wide range of stressors) and SOD (critical for addressing oxidative stress), as they are more directly related to stress. PK is related to carbohydrate metabolism (alterations in energy metabolism); VTG is associated with reproductive problems; Caspase mediates in apoptosis. Each gene in the toolkit plays a role depending on the type of stress assessed, and their combination provides a comprehensive understanding of the impacts of MPs. Differences in gene expressions between species and the exposure thresholds were found. These genes were examined in various scenarios with different types, concentrations, and sizes of MPs, alone or with other stressors. The toolkit offers significant advantages, allowing a comprehensive study of the impact of MPs and focusing on filtering bioindicator species, thus enabling pollution assessment and long-term monitoring. It will outperform traditional methods like tissue counts of MPs where only physical damage is visible, providing a deeper understanding. To our knowledge, this is the first toolkit of its kind.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107234"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct and transgenerational effects of simvastatin on the metabolism of the amphipod Gammarus locusta","authors":"João A. Rodrigues ,&nbsp;Raquel S. Chaves ,&nbsp;Miguel M. Santos ,&nbsp;Teresa Neuparth ,&nbsp;Ana M. Gil","doi":"10.1016/j.aquatox.2024.107221","DOIUrl":"10.1016/j.aquatox.2024.107221","url":null,"abstract":"<div><div>In this study, untargeted Nuclear Magnetic Resonance (NMR) metabolomics was applied for the first time, to our knowledge, to assess the metabolic impact of direct and transgenerational exposure (<em>F0</em> and <em>F3</em> generations, respectively) of amphipods <em>Gammarus locusta</em> to simvastatin (SIM), a pharmaceutical widely prescribed for the treatment of hypercholesterolemia. Results revealed the important gender-dependent nature of each of these effects. Directly exposed males showed enhanced glucose catabolism and tricarboxylic acid (TCA) cycle activity, in tandem with adaptations in osmotic regulation and glyoxylate metabolism. Exposed females exhibited only a small osmoregulatory effect. It is suggested that the response of exposed males may reflect reported high levels of methyl farnesoate hormone (low levels in females) and alterations in apical factors, namely decreased growth. Conversely, transgenerational effects were identified only in females, with impact on energy metabolism (glycolysis and TCA cycle enhancement) and osmoregulatory response. This expresses the ability of female gametes to transmit the effects of direct SIM exposure. Such effects were putatively related to reported delayed maturation and transcriptomic deviations impacting on carbohydrate and lipid metabolisms, possibly specifically engaging phenylalanine/tyrosine and choline in dopamine and choline metabolisms.</div><div>These findings reflect the importance of untargeted metabolomics in addressing not only direct exposure of contaminants, but also their transgenerational effects, potentially contributing towards improving hazard and risk assessment of biologically active compounds.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107221"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene transcription in the oyster Crassostrea gigas exposed to environmental concentrations of the UV filter Benzophenone-3","authors":"Miguel Saldaña-Serrano ,&nbsp;Jacó Joaquim Mattos ,&nbsp;Daína de Lima ,&nbsp;Mariana Rangel Pilotto ,&nbsp;Luiz Otávio de Barros Vilas Bôas ,&nbsp;Camila Pesenato Magrin ,&nbsp;Tâmela Zamboni Madaloz ,&nbsp;Camila Lisarb Velasquez Bastolla ,&nbsp;Diego José Nogueira ,&nbsp;Carlos Henrique Araujo de Miranda Gomes ,&nbsp;Flávia Lucena Zacchi ,&nbsp;Guilherme Razzera ,&nbsp;Gustavo Amadeu Micke ,&nbsp;Afonso Celso Dias Bainy","doi":"10.1016/j.aquatox.2025.107237","DOIUrl":"10.1016/j.aquatox.2025.107237","url":null,"abstract":"<div><div>Personal care products (PCPs), such as sunscreens, are usually found in various aquatic ecosystems at low concentrations (ng <span>l</span>^-1 to µg <span>l</span>^-1). However, there is limited information regarding their effects on marine bivalves. Therefore, the aim of this study was to evaluate the sublethal effects of environmental concentrations (1 and 100 µg <span>l</span>^-1) of benzophenone-3 (BP-3) in <em>Crassostrea gigas</em> oysters after 1 and 7 days of exposure. We analyzed the accumulation of BP-3 in the soft tissue of oysters and the transcription of genes associated with antioxidant enzyme (<em>SOD</em> and <em>CAT</em>), phase I biotransformation (<em>CYP356A1, CYP2AU2</em> and <em>CYP7A1</em>), phase II biotransformation (<em>GSTO.4A</em> and <em>GSTP.1.1</em>) and nuclear receptors (NR) that regulate gene transcription: <em>CgNR0B, CgNR1P10, CgNR1P11, CgNR2E2</em> and <em>CgNR5A</em>. The highest accumulation (34.9 ± 5.7 µg g^-1) was observed in oysters exposed for 7 days to 100 µg <span>l</span>^-1. Increased transcription of <em>SOD</em> and <em>CYP356A1</em> genes was observed in oysters exposed at both concentrations after 1 day of exposure. Additionally, <em>GSTP.1.1</em> transcription increased after 1 day of exposure to 100 µg <span>l</span>^-1 but decreased after 7 days of exposure to 1 µg <span>l</span>^-1. An increase in the transcription of <em>CgNR0B</em> and <em>CgNR1P10</em> genes was observed in oysters exposed to 100 µg <span>l</span>^-1 after 1 day of exposure, while only <em>CgNR5A</em> showed increased transcription after 1 and 7 days of exposure to 100 µg <span>l</span>^-1. Finally, the IBRv2i was significantly higher in the 100 µg <span>l</span>^-1 group compared to the control after 1 day of exposure. In conclusion, <em>C. gigas</em> exposed to concentrations that BP-3 could reach in nearshore areas exhibited variations in gene transcription, which could result in imbalances of physiological processes.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107237"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gibberellic acid (GA) induces developmental toxicity in zebrafish (Danio rerio) embryos via oxidative stress","authors":"Ying Wei ,&nbsp;Yan Gao ,&nbsp;Sida Zhang ,&nbsp;Yue Li ,&nbsp;Zuoying Wang ,&nbsp;Xu Zhang ,&nbsp;Zan Li ,&nbsp;Jinlian Li ,&nbsp;Ying Chen ,&nbsp;Dongmei Wu","doi":"10.1016/j.aquatox.2025.107247","DOIUrl":"10.1016/j.aquatox.2025.107247","url":null,"abstract":"<div><div>Gibberellic acid (GA) is a plant growth regulator that stimulates the growth of leaves and increases yield in agricultural production. However, GA is also regarded as an environmental endocrine disruptor, and its effect on aquatic life remains unclear. In this study, the toxic effects of GA on the development of zebrafish <em>(Danio rerio)</em> embryos were evaluated, and the mechanisms were revealed. The expression of genes related to development and function in zebrafish embryos at 96 h post fertilization (96 hpf) were detected by RT-qPCR method. Furthermore, the level of reactive oxygen species (ROS) and the expression of genes related to oxidative stress were detected. The results showed that the hatching and survival rates of zebrafish embryos were inhibited by 25 and 50 μmol/L GA, and the phenotype of pericardial edema was observed, indicating that GA may have cardiotoxicity on zebrafish embryos. Further RT-qPCR experiments showed that the above results may attributed to the down-regulation of <em>Myl7</em> and <em>Vmhc</em> genes. Besides, the phenotypes of liver degeneration, and the decrease of eye size were led by 10–50 μmol/L GA, along with the alteration of <em>Fabp10a, Gclc, Gsr, Gnat1</em>, and <em>Gnat2</em> genes, suggesting that GA may exhibit toxicities on liver and eye in zebrafish embryos. In addition, the phenotype of kidney edema and the up-regulation of <em>Kim1, Plce1</em>, and <em>Pkd2</em> genes were triggered by 50 μmol/L GA, indicating that GA may have toxic effect on kidney in zebrafish embryos. The level of ROS and the expression of genes related to oxidative stress were up-regulated under 10–50 μmol/L GA exposure, which may contribute to the developmental toxicity in zebrafish embryos. In summary, GA may affect the ecological environment of aquatic life, and its harm to aquatic ecology should be given special attention in the future.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107247"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse multigeneration combined effects of nano-sized plastics and mercury on growth and reproduction in a planktonic copepod Pseudodiaptomus annandalei","authors":"Dongmei Xie ,&nbsp;Chen Zhang ,&nbsp;De-Sheng Pei","doi":"10.1016/j.aquatox.2025.107256","DOIUrl":"10.1016/j.aquatox.2025.107256","url":null,"abstract":"<div><div>Nano-plastics (NPs) and heavy metals have attracted growing scientific attention because of both pollutants’ wide distribution and ecotoxicity. However, the long-term combined toxicity of NPs and mercury (Hg) on planktonic copepods, a crucial presence in marine environments, is unknown. Here, our study aimed to investigate the multigenerational phenotypic responses of the planktonic copepod <em>Pseudodiaptomus annandalei</em> to polystyrene NPs (about 50 nm) and Hg (alone or combined) at environmentally realistic concentrations (23 μg/L for NPs and 1 μg/L for Hg), and the underlying molecular mechanisms were explored. Despite the insignificant effect on survival, NPs could threaten the development and reproduction of <em>P. annandalei</em>, being ascribed to down-regulated genes in ingestive and reproductive functions. Hg exposure revealed inhibition of reproduction probably as an energy trade-off strategy. Importantly, in combined NPs and Hg, development and reproduction were further negatively impacted, even relative to NPs or Hg alone. Correspondingly, combined NPs and Hg presented the most pronounced transcriptomic response with a series of changes in cell functions and down-regulation of key genes in the DNA replication pathway and reproductive function as compared to NPs or Hg alone. The findings indicated adverse combined effects of NPs and Hg on <em>P. annandalei</em> under multigenerational scenarios, being a greater ecological risk for planktonic copepod than NPs or Hg alone. This study provides molecular insights into the long-term toxicity of combined NPs and Hg to planktonic copepods, underlining the increased risk in the population sustainability of marine zooplankton facing co-existing plastics and Hg pollution.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107256"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating microplastic contamination in Omani mangrove habitats using large mud snails (Terebralia palustris)","authors":"Muna Al-Tarshi ,&nbsp;John Husband ,&nbsp;Sergey Dobretsov","doi":"10.1016/j.aquatox.2024.107220","DOIUrl":"10.1016/j.aquatox.2024.107220","url":null,"abstract":"<div><div>This study investigated microplastic pollution in the large mud snail <em>Terebralia palustris</em> (Linnaeus, 1767) (Gastropoda: Potamididae) inhabiting the <em>Avicennia marina</em> mangrove ecosystems along the Sea of Oman. A modified digestion protocol, combining two methods, was employed to improve the detection of microplastics within the snail tissue. Results indicated that 50 % of the examined snails contained microplastics, with significant variability observed among different lagoons. Snails from the polluted Shinas lagoon exhibited higher levels of microplastics compared to those from the lowest polluted Al-Qurum Natural Reserve (MPA). The most prevalent type of microplastic in snail tissues was fibers, making up 75.7 % of the total. Fragments constituted about 24.2 %. Using portable Raman spectrometry, Polyurethane (PU) was identified as the predominant polymer, accounting for 50 % of the total. This was followed by Acrylic and Polyethylene, each representing 18.75 %, and Polyethylene Vynil Acetate (PEVA) at 12.50 %. Overall, it is clear that while snails do reflect the presence of microplastics (MPs) in their environment, their physical attributes do not strongly correlate with the levels or types of MPs they contain. Additionally, the significant difference between the abundance of MPs in sediment and in snails illustrates that, while snails may serve as general indicators of microplastic pollution, they may not be reliable as precise bioindicators or sentinel species for quantifying the extent of this pollution. Further studies are needed to explore other potential bioindicators in mangrove habitats.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107220"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The enzymatic and transcriptional adverse effects of hull in-water cleaning discharge on juvenile rockfish (Sebastes schlegeli)","authors":"Seong Hee Mun ,&nbsp;Kwang-Min Choi ,&nbsp;Dongju Shin ,&nbsp;Moonkoo Kim ,&nbsp;Taekhyun Kim ,&nbsp;Dae-Jung Kim ,&nbsp;Jee-Hyun Jung","doi":"10.1016/j.aquatox.2024.107191","DOIUrl":"10.1016/j.aquatox.2024.107191","url":null,"abstract":"<div><div>The hull in-water cleaning (IWC) process creates chemical contaminants, including antifouling paint particle mixtures that are directly discharged into the coastal environment. Recent attention has also been paid to the international regulation of ship hull cleaning discharges in environmental media. However, few studies have investigated the adverse effects or toxic pathways on resident marine species. In this study, we evaluated the chemical concentration of IWC discharge <em>in situ</em> and its toxic effects on juvenile rockfish (<em>Sebastes schlegeli</em>), a major coastal fishery resource, using enzymatic and transcriptomic studies. Zinc (8.05 ± 0.96 to 189.96 ± 47.76 μg/L) was the most abundant substance in IWC discharge, followed by copper (0.87 ± 0.19 to 1.97 ± 0.60 μg/L). No mortality was observed after 7 days of exposure in any experimental group; however, reactive oxygen species and acetylcholinesterase activity varied in juvenile rockfish exposed to the highest concentration of IWC discharge (10-fold-diluted IWC discharge). The immune and detoxification systems in juvenile rockfish exposed to IWC discharge were also significantly affected in juvenile rockfish exposed to 10-fold-diluted IWC discharge (zinc: 189.96 ± 47.76 μg/L, copper: 1.97 ± 0.60 μg/L). The expression of genes related to oxidative stress, including Cu/Zn-SOD, GST, and CAT, increased significantly in fish in all the exposure groups. Specifically, brain transcriptomic analysis revealed disturbances in the nervous system and homeostatic processes. Although lethal toxicity was not significantly affected, these findings indicate the potential hazard posed by sub-lethal concentrations of IWC discharge to juvenile fish, which are at a high-level in the food chain. Our enzymatic and transcriptomic results showed that Zn-dominant IWC discharge exposure may cause neuronal and immune toxicity in marine fish species, providing relevant insights into the management of hull IWC discharge to protect coastal ecosystems against chemical contaminants.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107191"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity of single and binary mixtures of copper and silica nanoparticles exposed to Nitzschia closterium f. minutissima","authors":"Peining Cai,&nbsp;Qi Li,&nbsp;Shuhui Wang,&nbsp;Liju Tan,&nbsp;Jiangtao Wang","doi":"10.1016/j.aquatox.2024.107211","DOIUrl":"10.1016/j.aquatox.2024.107211","url":null,"abstract":"<div><div>A large number of nanoparticles are produced and enter the aquatic environment, where they interact with each other, posing a potential threat to aquatic organisms. The toxicity of two types of nanoparticles (nCu and nSiO₂) on <em>Nitzschia closterium f. minutissima (N. closterium f. minutissima</em>) was investigated in this study by examining changes in microalgal cell density, instantaneous fluorescence rate (Ft), and a range of antioxidant parameters in the cells. It was found that both nCu and nSiO₂ showed time- and concentration-dependent toxic effects on <em>N. closterium f. minutissima</em>. nSiO₂ could promote microalgae growth at low concentrations by providing Si, an essential element for the synthesis of siliceous shells. As the exposure time increased, both the growth and photosynthetic efficiency of the microalgae were inhibited. Nanoparticles also produced oxidative stress and caused lipid peroxidation in the microalgae. In the meantime, SOD and CAT activity were altered to protect cells from oxidative damage. Inverted biomicroscopy images showed that the microalgae enhanced their cell size to adapt to the environmental stress as exposed to 1 mg/L nCu. Scanning electron microscope (SEM) images showed that 10 mg/L nSiO₂ could adsorb nCu and reduce the toxic effect of nCu on the microalgae, while 30 mg/L nSiO₂ caused mechanical damage to microalgal cells and accelerated the internalization of nanoparticles and Cu²⁺ in the cells.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107211"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics in commercial marine bivalves: Abundance, characterization and main effects of single and combined exposure","authors":"Daniela C.C. Silva ,&nbsp;João C. Marques ,&nbsp;Ana M.M. Gonçalves","doi":"10.1016/j.aquatox.2024.107227","DOIUrl":"10.1016/j.aquatox.2024.107227","url":null,"abstract":"<div><div>Microplastics (MPs) are persistent and ubiquitous pollutants in marine ecosystems, and they can be ingested and accumulated by marine organisms with economic value to humans, such as marine bivalves, which may pose a threat to the marine food chains and to human health. In this literature review, we summarized the recent findings on the abundance and main characteristics (shape, size, color, polymer) of MPs detected in valuable marine bivalve species. Furthermore, we surveyed the major impacts triggered by MP exposure, alone or in combination with other pollutants, in these organisms. Additionally, we discussed the methodologies, techniques and equipment commonly used by researchers for the determination of the abundance, characterization and effects of the MP particles in these organisms.</div><div>We verified that MPs have been widely detected in multiple species of commercial marine bivalves, with a great variety of shapes, sizes, colors and polymer types. In general, the methodologies used by researchers to determine the MP abundance in marine bivalves need to be harmonized to facilitate the comparability between studies. So far, previous research showed that the main effects of MPs, either alone or combined with other pollutants, on commercial marine bivalves include the induction of immunological, physiological and behavioral responses, reproductive modifications, genotoxicity and neurotoxicity, which were surveyed by using a wide variety of techniques and analytical equipment. In the future, researchers should focus on less studied bivalve species and should use the most precise and innovative methodologies to assess the effects of MPs and other pollutants on marine bivalves.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107227"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perfluorooctanoate and nano-titanium dioxide modulate male gonadal function in the mussel Mytilus coruscus","authors":"Bingyan Sun ,&nbsp;Yiting Pan ,&nbsp;Inna Sokolova ,&nbsp;Ying Shao ,&nbsp;Menghong Hu ,&nbsp;Youji Wang","doi":"10.1016/j.aquatox.2025.107251","DOIUrl":"10.1016/j.aquatox.2025.107251","url":null,"abstract":"<div><div>Perfluorooctanoic acid (PFOA) and nano-titanium dioxide (nano-TiO₂) are widely used in industrial applications such as manufacturing and textiles, and can be released into the environment, causing toxicity to marine organisms. To study the effects of these pollutants on the gonadal development, we exposed the males of <em>Mytilus coruscus</em> to varying PFOA concentrations (2 and 200 μg/L) alone or combined with nano-TiO₂ (0.1 mg/L, size: 25 nm) for 14 days. Co-exposure to PFOA and nano-TiO₂ resulted in a short-term (7 days) decrease in the gonadosomatic index (GSI), which recovered to baseline levels. In contrast, long-term (14 days) exposure induced changes in the testes, including increased protein content, decreased lipid content, reductions in spermatic area and sperm count, and elevated apoptotic cell levels. Furthermore, key genes essential for gonadal maturation were significantly upregulated after long-term exposure. PFOA and nano-TiO₂ can disrupt the gonadal function in the male mussels by interfering with Wnt family signaling pathways, modulation of steroid and lipid metabolism and induction of apoptosis. Therefore, PFOA and nanoparticle pollutants may pose a significant risk to the reproductive capacity of mussels’ populations from polluted coastal environments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107251"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}