Molecular and genotoxic effects of carbamazepine and methylmercury on the gonads of Dreissena polymorpha

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-07-14 DOI:10.1016/j.aquatox.2025.107493

Clément Baratange , Tainá Rocha de Almeida , Jean Armengaud , Isabelle Bonnard , Elise David , Laurence Delahaut , Véronique Gaillet , Emmanuel Guillon , Mélodie Kielbasa , Jean-Luc Loizeau , Nicolas Maurin , Stéphanie Sayen , Claudia Cosio

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引用次数: 0

Abstract

Carbamazepine (CBZ) and methylmercury (MeHg) are pervasive contaminants that share molecular toxicity pathways (e.g., neurotoxicity, oxidative stress, energy metabolism) and, in addition to their main toxicity target, are both suspected of reprotoxicity. Yet, the precise mechanisms behind these putative effects remain a subject of ongoing investigation. Dreissena polymorpha holds great potential for freshwater biomonitoring due to its wide distribution and filter-feeder lifestyle, but the influence of the reproductive stage for the biological response to pollutants is still understudied. Here, D. polymorpha males in the proliferation and maturation reproductive stages were exposed for 24 h to CBZ, MeHg and CBZ+MeHg. We combined the comet assay, proteomics and targeted RT-qPCR approaches to evaluate the impact of those exposures on the gonads. In the proliferation stage, both single and co-exposures modulated transcripts involved in antioxidant defenses. In the maturation stage, no significant modulation was observed with targeted approaches, most likely due to a higher background level masking the effect of pollutants. At both stages, differentially abundant proteins were involved in similar metabolic pathways, including the energy metabolism, antioxidant defenses and gonadal cell development. Proteomics also revealed modulation of proteins involved in genotoxicity pathways exclusively in the proliferation stage, aligning with the observation of an increase in genotoxic damage. MeHg exposure caused more alterations than co-exposure, in contrast with previous studies on other organs during similar exposure in D. polymorpha. This underlined the importance of assessing organ-specific responses to pollutants. Moreover, data confirmed a newly identified reprotoxic effect of MeHg, reinforcing the need to better consider the reproductive stage in biomonitoring programs using this species, and species with similar characteristics.

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卡马西平和甲基汞对多形鱼性腺的分子和基因毒性作用

卡马西平（CBZ）和甲基汞（MeHg）是普遍存在的污染物，它们具有共同的分子毒性途径（例如，神经毒性、氧化应激、能量代谢），除了它们的主要毒性靶点外，它们都被怀疑具有生殖毒性。然而，这些假设效应背后的确切机制仍然是一个正在进行调查的主题。多形溞分布广泛，具有滤食性生活方式，在淡水生物监测方面具有很大的潜力，但繁殖阶段对污染物生物反应的影响仍未得到充分研究。本研究采用CBZ、MeHg和CBZ+MeHg对处于增殖和成熟生殖阶段的多形瓢虫雄性进行24 h的暴露处理。我们结合彗星分析、蛋白质组学和靶向RT-qPCR方法来评估这些暴露对性腺的影响。在增殖阶段，单独和共同暴露均可调节参与抗氧化防御的转录本。在成熟阶段，目标方法没有观察到显著的调制，很可能是由于较高的背景水平掩盖了污染物的影响。在这两个阶段，差异丰富的蛋白质参与类似的代谢途径，包括能量代谢、抗氧化防御和性腺细胞发育。蛋白质组学还揭示了仅在增殖阶段参与遗传毒性途径的蛋白质调节，这与基因毒性损伤增加的观察结果一致。MeHg暴露比共暴露引起更多的改变，与先前的研究相比，在相似的暴露中，多形金蝇对其他器官的影响更大。这强调了评估器官对污染物的特异性反应的重要性。此外，数据证实了甲基汞的一种新发现的生殖毒性作用，这加强了在使用该物种和具有相似特征的物种的生物监测计划中更好地考虑生殖阶段的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.