Ziang Wang , Chenkai Ge , Wenbin Yuan , Wenwen Zha , Wanqing Chen , Weirong Li , Weitao Hu , Qinyuan Shen , Zhonghao Xiao , Xiaowen Shi , Huimin Li , Zigang Cao
{"title":"2-羟基蒽醌通过铁下垂诱导斑马鱼心脏毒性","authors":"Ziang Wang , Chenkai Ge , Wenbin Yuan , Wenwen Zha , Wanqing Chen , Weirong Li , Weitao Hu , Qinyuan Shen , Zhonghao Xiao , Xiaowen Shi , Huimin Li , Zigang Cao","doi":"10.1016/j.aquatox.2025.107485","DOIUrl":null,"url":null,"abstract":"<div><div>2-Hydroxyanthraquinone (2-hATQ) is a photochemical oxidation product of anthracene (ANT), a polycyclic aromatic hydrocarbon. ANT is listed as a priority pollutant by the US Environmental Protection Agency (EPA) due to its resistance to degradation and persistence in the environment. Because of ANT's characteristics, 2-hATQ can also be ubiquitously present in the environment and is more toxic than its parent compound ANT, posing significant risks to environmental safety and human health. Nevertheless, there are limited studies addressing its toxicological effects, particularly concerning cardiovascular toxicity. In the present study, zebrafish embryos acutely exposed to 2-hATQ (0, 0.4, 0.8, and 1.2 mg/L) at different concentrations showed significant cardiac developmental abnormalities, as evidenced by slowing of the heart rate, pericardial edema, and the cardiac malformations. Transcriptomic sequencing analysis and quantitative real-time polymerase chain reaction (qPCR) study showed that 2-hATQ induced cardiac ferroptosis via disruption of the antioxidant system, glutathione depletion, increase in polyunsaturated fats and accumulation of iron ions. Co-exposure of ferroptosis inhibitor (Fer-1) effectively rescues the morphological abnormalities of the heart. After exposing adult zebrafish to low concentration 2-hATQ for 28 days, ventricular morphology was reduced and internal structures showed partial vacuolation and fibrosis. This study reveals for the first time the negative influence of 2-hATQ exposure on the cardiac development of aquatic organisms and this discovery may offer significant insights and references for an in-depth understanding of 2-hATQ and other ANT-related environmental risk substances.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"286 ","pages":"Article 107485"},"PeriodicalIF":4.3000,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"2-Hydroxyanthraquinone induces cardiotoxicity in zebrafish via ferroptosis\",\"authors\":\"Ziang Wang , Chenkai Ge , Wenbin Yuan , Wenwen Zha , Wanqing Chen , Weirong Li , Weitao Hu , Qinyuan Shen , Zhonghao Xiao , Xiaowen Shi , Huimin Li , Zigang Cao\",\"doi\":\"10.1016/j.aquatox.2025.107485\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>2-Hydroxyanthraquinone (2-hATQ) is a photochemical oxidation product of anthracene (ANT), a polycyclic aromatic hydrocarbon. ANT is listed as a priority pollutant by the US Environmental Protection Agency (EPA) due to its resistance to degradation and persistence in the environment. Because of ANT's characteristics, 2-hATQ can also be ubiquitously present in the environment and is more toxic than its parent compound ANT, posing significant risks to environmental safety and human health. Nevertheless, there are limited studies addressing its toxicological effects, particularly concerning cardiovascular toxicity. In the present study, zebrafish embryos acutely exposed to 2-hATQ (0, 0.4, 0.8, and 1.2 mg/L) at different concentrations showed significant cardiac developmental abnormalities, as evidenced by slowing of the heart rate, pericardial edema, and the cardiac malformations. Transcriptomic sequencing analysis and quantitative real-time polymerase chain reaction (qPCR) study showed that 2-hATQ induced cardiac ferroptosis via disruption of the antioxidant system, glutathione depletion, increase in polyunsaturated fats and accumulation of iron ions. Co-exposure of ferroptosis inhibitor (Fer-1) effectively rescues the morphological abnormalities of the heart. After exposing adult zebrafish to low concentration 2-hATQ for 28 days, ventricular morphology was reduced and internal structures showed partial vacuolation and fibrosis. This study reveals for the first time the negative influence of 2-hATQ exposure on the cardiac development of aquatic organisms and this discovery may offer significant insights and references for an in-depth understanding of 2-hATQ and other ANT-related environmental risk substances.</div></div>\",\"PeriodicalId\":248,\"journal\":{\"name\":\"Aquatic Toxicology\",\"volume\":\"286 \",\"pages\":\"Article 107485\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-07-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquatic Toxicology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0166445X25002498\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquatic Toxicology","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0166445X25002498","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
2-Hydroxyanthraquinone induces cardiotoxicity in zebrafish via ferroptosis
2-Hydroxyanthraquinone (2-hATQ) is a photochemical oxidation product of anthracene (ANT), a polycyclic aromatic hydrocarbon. ANT is listed as a priority pollutant by the US Environmental Protection Agency (EPA) due to its resistance to degradation and persistence in the environment. Because of ANT's characteristics, 2-hATQ can also be ubiquitously present in the environment and is more toxic than its parent compound ANT, posing significant risks to environmental safety and human health. Nevertheless, there are limited studies addressing its toxicological effects, particularly concerning cardiovascular toxicity. In the present study, zebrafish embryos acutely exposed to 2-hATQ (0, 0.4, 0.8, and 1.2 mg/L) at different concentrations showed significant cardiac developmental abnormalities, as evidenced by slowing of the heart rate, pericardial edema, and the cardiac malformations. Transcriptomic sequencing analysis and quantitative real-time polymerase chain reaction (qPCR) study showed that 2-hATQ induced cardiac ferroptosis via disruption of the antioxidant system, glutathione depletion, increase in polyunsaturated fats and accumulation of iron ions. Co-exposure of ferroptosis inhibitor (Fer-1) effectively rescues the morphological abnormalities of the heart. After exposing adult zebrafish to low concentration 2-hATQ for 28 days, ventricular morphology was reduced and internal structures showed partial vacuolation and fibrosis. This study reveals for the first time the negative influence of 2-hATQ exposure on the cardiac development of aquatic organisms and this discovery may offer significant insights and references for an in-depth understanding of 2-hATQ and other ANT-related environmental risk substances.
期刊介绍:
Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems.
Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants
The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.