Aquatic Toxicology最新文献

{"title":"Transgenerational epigenetic inheritance in Daphnia magna exposed to polyethylene microplastic fragments containing benzophenone-3 additive","authors":"Jinyoung Song ,&nbsp;Min-Sub Kim ,&nbsp;Nik Nurhidayu Nik Mut ,&nbsp;Jae-Seong Lee ,&nbsp;Jinho Jung","doi":"10.1016/j.aquatox.2026.107747","DOIUrl":"10.1016/j.aquatox.2026.107747","url":null,"abstract":"<div><div>Microplastic (MP) pollution has emerged as a ubiquitous environmental threat, yet long-term transgenerational consequences remain poorly understood. This study investigated transgenerational epigenetic inheritance in <em>Daphnia magna</em> following exposure to polyethylene MP fragments containing benzophenone-3 (MP/BP-3). Four generations (F0–F3) were examined for 21 days each, with only F0 directly exposed to MP/BP-3. Population growth rates showed significant decreases in F0–F1, followed by recovery in F2–F3. Whole genome bisulfite sequencing revealed 492, 357, 351, and 681 differentially methylated genes in F0, F1, F2, and F3 generations, respectively. Remarkably, six genes remained hypomethylated through all four generations, including PAPSS1, ELP1, PRPL20, and IAPS5, encoding proteins involved in detoxification, development, and cellular stress, and cell adhesion. GO and KEGG pathway analyses consistently identified ‘cell adhesion’ and cardiovascular-related pathways as enriched through all generations, suggesting persistent predisposition to cardiac dysfunction. These findings demonstrate that single-generation MP exposure creates lasting molecular signatures that persist through unexposed offspring, fundamentally challenging current environmental risk assessment frameworks.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107747"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146134427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilevel toxicological impacts of industrial settleable particulate matter in a freshwater crustacean, Macrobrachium rosenbergii","authors":"Michelly Pereira Soares ,&nbsp;Carolina Fernandes De Angelis ,&nbsp;Ludmila Mendes Silva ,&nbsp;Beatriz Helena Montanari ,&nbsp;Iara da Costa Souza ,&nbsp;Magdalena V. Monferrán ,&nbsp;Daniel A. Wunderlin ,&nbsp;Marisa Narciso Fernandes ,&nbsp;Cléo Alcantara Costa Leite","doi":"10.1016/j.aquatox.2026.107714","DOIUrl":"10.1016/j.aquatox.2026.107714","url":null,"abstract":"<div><div>As integral components of aquatic food webs, crustaceans serve as pivotal conduits for energy transfer and sensitive bioindicators of environmental quality. This study characterizes the toxicological consequences of settleable atmospheric particulate matter (SePM)—a byproduct of industrial iron-ore processing—utilizing the freshwater shrimp <em>Macrobrachium rosenbergii</em> as a model organism. Experimental exposure demonstrated that SePM significantly enhances the aqueous solubility of both conventional (Fe, Al, Zn, Cu, Mn) and emergent (Cr, Ni, Sr, Sn, La, Ce) metals, establishing it as a potent vector for multi-elemental contamination. Exposed individuals bioaccumulated 24 metals, with predominant enrichment of Fe, Ba, Sr, Cu, and Zn; these accumulation profiles were modulated by both external concentration and element-specific uptake kinetics. Notably, ultrastructural characterization revealed significant metal sequestration within the exoskeleton, identifying an incidental pathway for contaminant cycling through discarded carapaces (ecdysis). Biochemical assessments indicated impairment of redox homeostasis, characterized by the exhaustion of enzymatic (CAT, SOD, GPx, GST) and non-enzymatic (GSH) defenses, alongside elevated lipid peroxidation and DNA strand breaks. While bioaccumulation had a dose-dependent progression, oxidative distress was manifest even at the lowest exposure levels, underscoring that the toxicity of SePM as a complex mixture cannot be accurately predicted by the additive impacts of its individual constituents. These findings highlight SePM as a critical, yet largely overlooked, driver of air-to-water cross-contamination. Our results underscore the urgent need to incorporate such atmospheric deposition into environmental monitoring frameworks and to expand regulatory criteria to address emergent metals and potential synergistic effects of metallic mixtures.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107714"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145961725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute low-dose benzo[a]pyrene exposure induces neurodegenerative syndrome in cyp1a low-expression zebrafish: A novel living Bioindicator for dioxin-like environmental pollutants","authors":"Min Li ,&nbsp;Xiaoping Huang ,&nbsp;Dongjie Wang ,&nbsp;Zhenhan Chen ,&nbsp;Shulan Wang ,&nbsp;Xian Qing ,&nbsp;Shaolin Xie","doi":"10.1016/j.aquatox.2026.107743","DOIUrl":"10.1016/j.aquatox.2026.107743","url":null,"abstract":"<div><div>Benzo[a]pyrene (BaP), a ubiquitous environmental polycyclic aromatic hydrocarbon (PAH), elicits toxicity that is closely linked to the activity of the key metabolic enzyme CYP1A. In this study, a <em>cyp1a</em> low-expression zebrafish strain, KI (cyp1a^+/+-T2A-mCherry), was used to investigate the toxic effects and mechanisms of low-dose BaP exposure under conditions of <em>cyp1a</em> deficiency. The results revealed that <em>cyp1a</em> low expression significantly exacerbated BaP-induced mortality, growth inhibition, apoptosis, and oxidative stress. It also induced neurodegenerative lesions accompanied by reduced acetylcholine (ACh) levels. Behavioral assessments showed that <em>cyp1a</em> low expression zebrafish displayed anxiety-like behaviors, altered locomotor activity, and visual impairment following exposure—effects potentially associated with the down regulation of opsin genes (e.g., <em>opn1sw1, opn1sw2</em>). Transcriptomic profiling further identified substantial differences in gene expression between <em>cyp1a</em> low expression and wild-type zebrafish after BaP treatment. KEGG pathway analysis highlighted enrichments related to neurodegenerative diseases, cell cycle arrest, and apoptosis in the <em>cyp1a</em> low expression group, along with dysregulated expression of neurodegeneration-associated genes such as APPa, APPb, PSEN2, and TUBB. Notably, this study is the first to report that <em>cyp1a</em> low expression exhibit enhanced behavioral sensitivity to trace levels of dioxin-like contaminants (10⁻¹³ M TCDD). When exposed to polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs), this model demonstrated pronounced and quantifiable changes in locomotor behavior, underscoring its potential as a highly sensitive novel biosensor for environmental monitoring. Overall, this work not only confirms that <em>cyp1a</em> deficiency markedly increases susceptibility to BaP-induced neurodegeneration in zebrafish but also establishes the <em>cyp1a</em> low-expression model as a transformative tool offering novel biomarkers for risk assessment and early warning of dioxin-like pollutants. It significantly advances the sensitivity and specificity of environmental toxicology research, providing new technical and theoretical foundations for the field.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107743"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146071814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel approaches methodologies in ecotoxicology - Metabolism reveals multigenerational effects of pollutants on daphnids","authors":"Anna Michalaki ,&nbsp;Anne Leung ,&nbsp;Emma Rowan ,&nbsp;Ya Gao ,&nbsp;Claire Connolly ,&nbsp;Lorraine Brennan ,&nbsp;Konstantinos Grintzalis","doi":"10.1016/j.aquatox.2026.107746","DOIUrl":"10.1016/j.aquatox.2026.107746","url":null,"abstract":"<div><div>The field of ecotoxicology is advancing with the use of new approaches methodologies (NAMs) to scrutinize the effects of pollutants on aquatic ecosystems. This study employs cutting-edge methods to assess the impact of mixtures of eight pollutants on <em>Daphnia magna</em>, a keystone species in freshwater ecosystems. The levels of toxicity were in the same order of magnitude for the eight stressors, while the mixture was significantly more toxic. For the present study, daphnids were exposed for five consecutive generations to seven non-lethal and environmentally relevant concentrations ranging from 1 ng/l to 1000 μg/l. During the first generation, only the higher concentrations affected the enzymatic activities of daphnids, while after the third generation the effects were milder. In the fifth generation all concentrations, even the lowest, caused an impact on daphnids, while the animals exposed to the 1000 μg/l did not survive. Additionally, a feeding assay on D2 daphnids revealed a dose-dependent effect caused by the higher concentrations 500 and 1000 μg/l. Besides the biochemical markers, a targeted LC-MS/MS approach on the fifth generation daphnids confirmed the results from the former revealing a significant dose-dependent pattern even at the lower concentrations 1–100 ng/l. Our findings underscore the importance of not only acute or one generation chronic exposure, but multigenerational chronic exposures to investigate how the stress is carried out to the next generations.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107746"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146134428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative proteomics and metabolomics approach to elucidate the multi-organ developmental toxicity of decabromodiphenyl ethane in zebrafish larvae","authors":"Ruiyang Zhang ,&nbsp;Yue Zhang ,&nbsp;Jinglong Xue ,&nbsp;Ruxuan Zhang ,&nbsp;Junhong Xie ,&nbsp;Xinyue Luo ,&nbsp;Xi Yang ,&nbsp;Hongou Wang ,&nbsp;Jianhui Liu ,&nbsp;Jialiu Wei ,&nbsp;Xianqing Zhou","doi":"10.1016/j.aquatox.2026.107712","DOIUrl":"10.1016/j.aquatox.2026.107712","url":null,"abstract":"<div><div>Decabromodiphenyl ethane (DBDPE), as the novel brominated flame retardant, has been frequently detected in aquatic environments worldwide and has been confirmed to be harmful to aquatic organisms. Previous studies have mostly inferred potential damage to specific organs based on abnormal behaviors in zebrafish, but the multi-organ cross-toxicity of DBDPE and its underlying mechanisms remain to be elucidated. In this study, zebrafish (<em>Danio rerio</em>) embryos were exposed to DBDPE at concentrations of 5, 50, and 500 μg/L for 120 h. The results showed that DBDPE impaired embryonic development, causing cardiotoxicity, neurotoxicity, and visual toxicity. DBDPE induced excessive production of reactive oxygen species (ROS), accumulation of malondialdehyde (MDA), and depletion of glutathione (GSH), which in turn triggered phosphatidylethanolamine (PE) accumulation and ferroptosis-related proteins (ceruloplasmin, heme oxygenase 1, and autophagy-related protein 5) downregulation, accompanied by cardiac developmental abnormalities such as pericardial edema, increased heart rate, and prolonged sinoatrial-atrioventricular (SV-BA) distance. Beyond its cardiac effects, DBDPE exposure induced distinct neurotoxicity, including anxiety and aggression. Mechanistically, DBDPE directly interfered with lysophosphatidylcholine (LysoPC) metabolism and disrupted the normal localization of metabotropic glutamate receptor 5 (mGluR5), leading to neurobehavioral abnormalities. In addition, the phenomenon of ocular protrusion may be related to abnormal expression of retinol dehydrogenase 12 (RDH12) and glucuronosyltransferase (UGT1B2), key factors in retinol metabolism. This study firstly showed that DBDPE's multi-organ developmental toxicity is driven by an interconnected metabolic-protein network, which not only significantly advances the understanding of the complex toxic mechanisms of DBDPE, but also lays a solid scientific foundation for accurate environmental risk assessment and early health warning of such emerging pollutants.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107712"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145957003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking aquaculture practices to antibiotic occurrence, accumulation, and environmental risk in freshwater systems","authors":"Ruta Medne ,&nbsp;Ilga Kokorīte ,&nbsp;Vadims Bartkevics ,&nbsp;Anete Kubliņa ,&nbsp;Anete Niemi ,&nbsp;Haralds Plostiņš ,&nbsp;Maruta Vehi ,&nbsp;Sandra Mingarelli","doi":"10.1016/j.aquatox.2026.107744","DOIUrl":"10.1016/j.aquatox.2026.107744","url":null,"abstract":"<div><div>Aquaculture is a rapidly expanding component of global food production, but its intensification raises environmental concerns due to the widespread use of antibiotics to control bacterial diseases. This study examined the occurrence, distribution, and ecological risks of antibiotic residues in freshwater ecosystems downstream of aquaculture facilities in Latvia. Twenty-three antibiotics were analyzed in outflows, sediments, and biota using UHPLC–MS/MS. Four compounds—enrofloxacin, doxycycline, oxytetracycline, and florfenicol—were detected, with enrofloxacin and doxycycline showing the highest detection frequencies and concentrations in the outflows (up to 1315 ng L⁻¹ and 775 ng L⁻¹, respectively). Substantial accumulation occurred in sediments (up to 13,811 µg kg⁻¹ for enrofloxacin) and molluscs (up to 97 µg kg⁻¹ for doxycycline), suggesting persistence and potential bioaccumulation. Risk quotient (RQ) analysis indicated high environmental risk (RQ ≥ 1) in 10% of outflows, mainly associated with enrofloxacin. Extremely high RQs were observed in sediments (11–9086) and molluscs (43–200) for all detected antibiotics. Antibiotic levels and occurrence patterns varied significantly among aquaculture types, with juvenile salmonid farms identified as key sources of pollution. These findings highlight freshwater aquaculture as an under-recognized source of antibiotic contamination in the Baltic region and underscore the need for enhanced monitoring, transparent reporting, and stricter regulations to mitigate antimicrobial pollution and resistance development in aquatic environments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107744"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146071813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomics and targeted metabolomics uncover concomitant gut microbiota dysbiosis and bile acid metabolism alteration in norfloxacin-exposed Bufo gargarizans tadpoles","authors":"Yanjiao Song ,&nbsp;Xiuling Song ,&nbsp;Xiaoli Liu ,&nbsp;Ling Jiang ,&nbsp;Lihong Chai","doi":"10.1016/j.aquatox.2026.107742","DOIUrl":"10.1016/j.aquatox.2026.107742","url":null,"abstract":"<div><div>Norfloxacin (NOR) is a fluoroquinolone antibiotic widely detected in aquatic environments, yet little is known about its toxic effects on amphibians. Bile acids (BAs) are crucial metabolites derived from gut microbiota-host co-metabolism and play vital roles in maintaining host health. BA composition is regulated by the gut microbiota through specific enzymes: bile salt hydrolases (BSHs) deconjugate primary BAs; bile acid-inducible enzymes (BAIs) and hydroxysteroid dehydrogenases (HSDHs) then convert them into secondary BAs. This study investigated the effects of NOR on <em>Bufo gargarizans</em> tadpoles using a combination of intestinal-targeted BA metabolomics, metagenomics, and histopathological analysis. Tadpoles were exposed to 10 and 100 μg/L NOR from Gs26 to Gs36, with 4 independent biological replicates per group. Our results showed that NOR exposure significantly increased the relative abundance of gut microbiota encoding BAIs, HSDHs, and/or BSHs, which was accompanied by a decrease in the ratios of primary/secondary BAs and conjugated/deconjugated BAs. Meanwhile, NOR treatment elevated antibiotic resistance gene abundance and induced intestinal histopathological alterations in tadpoles, characterized by reduced epithelial cell height and hypertrophy of smooth muscle cells (SMCs). In summary, environmentally relevant concentrations (10 and 100 μg/L) of NOR affected the intestinal microbiota, thereby disrupting BAs biotrasformation, ultimately potentially compromising intestinal health in tadpoles. This highlighted the potential ecological risks posed by NOR pollution in aquatic ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107742"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146071815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoring the waters while polluting the food web – the unintended consequences of aluminum use in lake management","authors":"Michał Rybak ,&nbsp;Yağmur Bengisu Güngör ,&nbsp;Zofia Książkiewicz ,&nbsp;Oskar Kamiński ,&nbsp;Aleksandra Proch ,&nbsp;Magdalena Woźniak ,&nbsp;Przemysław Niedzielski","doi":"10.1016/j.aquatox.2026.107750","DOIUrl":"10.1016/j.aquatox.2026.107750","url":null,"abstract":"<div><div>Aluminum-based coagulants are widely used to immobilize phosphorus during lake restoration, yet their transfer through aquatic food webs and potential effects on elemental homeostasis remain poorly resolved. We conducted microcosm experiments with the submerged macrophyte <em>Myriophyllum spicatum</em> and the freshwater snail <em>Lymnaea stagnalis</em> to test whether polyaluminum chloride (PAC), applied at restoration-relevant doses of 12.9 mg Al dm^-3 and 24.9 mg Al dm^-3, results in aluminum accumulation and associated stoichiometric disturbances. We explicitly separated two exposure routes: assessing trophic transfer (dietary exposure) by feeding snails macrophytes previously treated with PAC, and quantifying environmental exposure by exposing snails directly to PAC in the water column. Our findings reveal that Al treatments significantly altered short-term growth and the elemental composition of macrophytes, implying potential long-term consequences for nutrient cycling and ecosystem functioning. Moreover, both direct and dietary exposure routes lead to Al accumulation in snails, accompanied by imbalanced nutrient uptake, even in the absence of significant acidification during the initial phase of coagulant application, which is considered environmentally safe. These findings raise important questions about the presumed ecological neutrality of chemical restoration techniques and highlight the need for a more critical evaluation of their widespread application. By revealing the often-overlooked environmental costs of a widely implemented geoengineering practice, this study underscores the imperative for comprehensive, ecosystem-wide risk assessments in the management of aquatic environments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107750"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multigenerational exposure to the fungicide tebuconazole reveals hormetic effects on the demography and biology of a non-target vector mosquito Aedes albopictus","authors":"Hafiz Azhar Ali Khan ,&nbsp;Tiyyabah Khan","doi":"10.1016/j.aquatox.2026.107752","DOIUrl":"10.1016/j.aquatox.2026.107752","url":null,"abstract":"<div><div>The use of fungicides is an integral part of field crops cultivation for the management of several diseases; however, the potential for fungicides to exert sublethal impacts on the biology of non-target insect pests is an understudied area with serious management consequences. <em>Aedes albopictus</em> is one of the most common human disease vectors and non-target aquatic insect species inhabited in and/or around several field crops. Here, we studied the effects of tebuconazole on several biological traits of <em>Ae. albopictus</em>. Biological traits and population parameters of a strain of <em>Ae. albopictus</em> were studied after exposing it to the LC₅ (62.09 mg L^-1), LC₁₀ (86.31 mg L^-1), or LC₂₀ (128.62 mg L^-1) level of tebuconazole for five consecutive generations and were compared with an unexposed control group. The results revealed that LC₅ and LC₁₀ levels had hormetic (stimulatory) effects, while the LC₂₀ level posed non-hormetic (inhibitory) effects on the performance of most of the life history parameters. The developmental period of egg, larval, and pupal stages of the LC₅ and LC₁₀ groups were faster than in the LC₂₀ and control groups. The performance of biological parameters of the LC₅ and LC₁₀ groups such as preadult and adult survival rates, preoviposition period, oviposition days, and fecundity was better than that of the LC₂₀ and control groups. The mean generation time was significantly shortened in the LC₅ and LC₁₀ groups. Moreover, higher values of the net reproductive rate (<em>R₀</em>) and finite rate of increase (<em>λ</em>) were recorded in the LC₅ and LC₁₀ groups than in the LC₂₀ and control groups. In conclusion, the findings of the present study revealed that exposure of <em>Ae. albopictus</em> to sublethal levels of tebuconazole may favor its rapid development, enhanced fecundity, survival and population expansion under field conditions, thereby increasing the probability of <em>Ae. albopictus</em>-borne infections.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107752"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of potential mechanisms of chronic copper effects on reproduction in zebrafish (Danio rerio)","authors":"Kevin V. Brix,&nbsp;Jeffrey C. Wolf,&nbsp;Stijn Baken,&nbsp;Tara Miller,&nbsp;Yamini Gopalapillai,&nbsp;Douglas J. Fort","doi":"10.1016/j.aquatox.2025.107698","DOIUrl":"10.1016/j.aquatox.2025.107698","url":null,"abstract":"<div><div>This study investigated copper (Cu) effects on zebrafish (<em>Danio rerio</em>) reproduction. Multiple measured endpoints provided insight into potential modes of action. Reproductively active zebrafish were exposed to dissolved Cu concentrations of 0.3 (control), 6.2, 16.8, and 48.8 μg L⁻¹ for 21 days, generally following OECD TG 229. Parameters assessed included fecundity, gonado- and liver-somatic index (GSI and LSI), plasma vitellogenin, and two indicators of oxidative stress – catalase (CAT) and glutathione peroxidase (GSH-Px) concentration on days 0, 2, 10, and 21. Additionally, histopathological evaluation of the gill, liver, and gonad was undertaken at test termination. Copper significantly reduced fecundity at 16.8 and 48.8 μg L⁻¹ Cu. Correspondingly, CAT and GSH-Px concentrations increased in a concentration- and time-dependent manner in the gonad and liver indicating oxidative stress in these tissues. Plasma vitellogenin concentrations, GSI, and LSI were not affected by Cu exposure. Liver histopathology indicated treatment-related increases in bile duct hyperplasia in male fish. Absence of ovarian post-ovulatory follicles in 16.8 and 48.8 μg L⁻¹ group females was consistent with reduced fecundity, but other histopathological findings in gonads were not considered conclusively related to Cu exposure. Overall, the absence of changes in plasma vitellogenin concentrations and endocrine-specific histopathological effects provides further evidence against an endocrine disruption adverse outcome pathway. The lack of overt histopathological damage in the gonads indicates oxidative damage is also likely not driving effects on reproduction. Alternative modalities, such as Cu-induced changes in bioenergetics, could be playing a role in observed reproductive effects and could be investigated.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"292 ","pages":"Article 107698"},"PeriodicalIF":4.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145893697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}