Aquatic Toxicology最新文献

{"title":"Impact of copper nanoparticles (CuNPs) on gonadal development in zebrafish larvae and melatonin therapeutic intervention","authors":"Zhilong Liu ,&nbsp;Yewei Zhang ,&nbsp;Xiaojun Jing ,&nbsp;Shengyan Su","doi":"10.1016/j.aquatox.2025.107484","DOIUrl":"10.1016/j.aquatox.2025.107484","url":null,"abstract":"<div><div>Copper nanoparticles (CuNPs), owing to their high specific surface area and reactivity, are extensively applied across various fields while concurrently posing certain hazards to aquatic organisms. This study comprehensively investigated the detrimental effects of CuNPs on the reproductive system of zebrafish and emphasized the examination of the potential therapeutic role of melatonin. The research found that CuNPs interfere with zebrafish gonadal development through oxidative damage, endocrine disruption (upregulated estradiol, E₂; downregulated testosterone, T), and suppression of reproduction-related genes, consequently causing impaired germ cell development and even organismal mortality. Through transcriptomic research, we discovered that CuNPs induce gonadal oxidative stress (Oxidative phosphorylation pathway) and endoplasmic reticulum stress (Protein processing in endoplasmic reticulum pathway), downregulate <em>zgc:153,993</em> to activate the mitochondrial apoptosis pathway, and inhibit the <em>hsp70l</em>-MAPK/ERK feedback loop to amplify damage; the organism compensatorily upregulated <em>cyp2x12</em> to enhance detoxification function and upregulated <em>dync1i1/dync1li2</em> to activate the Phagosome pathway to clear aberrant apoptotic products. Melatonin, by antagonizing ROS and repairing stress-induced damage, modulated the expression of most key gonadal development genes to restore homeostasis. Simultaneously, it transcriptionally upregulated ribosome biogenesis gene (<em>si:dkey-103j14.5</em>) and lysosomal pathway gene (<em>si:ch211–122f10.4</em>) to alleviate nucleic acid oxidation damage and clear damaged substrates. Finally, by upregulating <em>got2a</em> to activate the aspartate metabolism pathway, it achieved multi-target therapeutic intervention against gonadal injury under CuNPs exposure. Our study reveals the physiological and molecular mechanisms underlying the reproductive toxicity of CuNPs; melatonin, as an endogenous protective agent, shows promise in mitigating the ecotoxicological effects of CuNPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107484"},"PeriodicalIF":4.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deleterious effects of in vivo parameters and oxidative stress-mediated autophagy and apoptosis induction via MAPKs and AMPK signaling pathways in the ammonia-exposed marine rotifer Brachionus plicatilis","authors":"Seong Chan Yun ,&nbsp;Deok-Seo Yoon ,&nbsp;Yoseop Lee ,&nbsp;Haksoo Jeong ,&nbsp;Yoshitaka Sakakura ,&nbsp;Zhou Yang ,&nbsp;Jae-Seong Lee ,&nbsp;Jin-Sol Lee","doi":"10.1016/j.aquatox.2025.107491","DOIUrl":"10.1016/j.aquatox.2025.107491","url":null,"abstract":"<div><div>In this study, we investigated the molecular mechanisms responsible for the physiological and behavioral consequences of ammonia exposure in the marine rotifer <em>Brachionus plicatilis</em>, a representative model of aquatic invertebrates. <em>B. plicatilis</em> is particularly susceptible to ammonia, which can induce substantial increases in antioxidant enzyme activities, including those of superoxide dismutase and catalase, along with reactive oxygen species. Furthermore, the expression of mitogen-activated protein kinases (MAPKs), a critical pathway implicated in oxidative stress, was investigated using western blots to elucidate increases in the phosphorylation of p38 among MAPKs. We also identified an adverse behavioral effect of ammonia exposure in the form of a reduction in swimming speed in <em>B. plicatilis</em>. Following this, the activity of acetylcholinesterase and expression of 5ʹ-adenosine monophosphate-activated protein kinase (AMPK) proteins were investigated to determine the relationship between deleterious behavioral effects and molecular responses. Western blots were also used to identify autophagy and apoptosis as molecular side effects of ammonia exposure in rotifers. Increased expression of relevant biomarkers indicated that autophagy and apoptosis occurred along with increased phosphorylation of p38 and AMPK among MAPKs. This study offers a deeper understanding of the impact of ammonia on marine organisms by investigating alterations at the molecular level, as well as physiological changes and behavioral patterns of rotifers in response to varying concentrations of ammonia.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107491"},"PeriodicalIF":4.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of TKTD models in predicting time-resolved bioaccumulation and toxicity of pesticides to aquatic organisms","authors":"Huizhen Li,&nbsp;Qingping Lan,&nbsp;Jing You","doi":"10.1016/j.aquatox.2025.107489","DOIUrl":"10.1016/j.aquatox.2025.107489","url":null,"abstract":"<div><div>Pesticides play a crucial role in global food production, but their extensive use has led to increasing threats to aquatic ecosystems due to their ubiquitous occurrence in aquatic ecosystems and high toxic potency to non-target organisms. Conventional ecological risk assessment (ERA) approaches, which mainly rely on static external exposure thresholds (e.g., LC50s), are often inadequate for accurately predicting pesticide risks in environmentally realistic scenarios where pesticide exposures fluctuate dynamically due to intermittent pesticide applications and hydrological events. Recent advances advocate for a paradigm shift toward internal exposure-based metrics and mechanistic toxicokinetic-toxicodynamic (TKTD) modeling approaches to address these challenges. These models provide a robust framework for quantifying pesticide uptake, internal distribution, metabolism, and elimination processes in organisms (TK), and simultaneously characterize the time-dependent biological effects (TD). The present review synthesizes current applications of TKTD modeling in predicting bioaccumulation and toxicity of pesticides in aquatic organisms. The influences of chemical properties (e.g., hydrophobicity, metabolic potency) and biological traits (e.g., body size) on bioaccumulation potential of pesticides in aquatic organisms are evaluated. We further discuss the application of TD models in predicting time-resolved effects (e.g., delayed and carry-over effects) and species sensitivity. By integrating these mechanistic insights into aquatic risk assessment approaches and regulatory needs, a TKTD-based ERA framework is recommended for advancing pesticide risk assessments, particularly for addressing the challenges under realistic and fluctuating exposure conditions in natural aquatic ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"286 ","pages":"Article 107489"},"PeriodicalIF":4.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2-Hydroxyanthraquinone induces cardiotoxicity in zebrafish via ferroptosis","authors":"Ziang Wang ,&nbsp;Chenkai Ge ,&nbsp;Wenbin Yuan ,&nbsp;Wenwen Zha ,&nbsp;Wanqing Chen ,&nbsp;Weirong Li ,&nbsp;Weitao Hu ,&nbsp;Qinyuan Shen ,&nbsp;Zhonghao Xiao ,&nbsp;Xiaowen Shi ,&nbsp;Huimin Li ,&nbsp;Zigang Cao","doi":"10.1016/j.aquatox.2025.107485","DOIUrl":"10.1016/j.aquatox.2025.107485","url":null,"abstract":"<div><div>2-Hydroxyanthraquinone (2-hATQ) is a photochemical oxidation product of anthracene (ANT), a polycyclic aromatic hydrocarbon. ANT is listed as a priority pollutant by the US Environmental Protection Agency (EPA) due to its resistance to degradation and persistence in the environment. Because of ANT's characteristics, 2-hATQ can also be ubiquitously present in the environment and is more toxic than its parent compound ANT, posing significant risks to environmental safety and human health. Nevertheless, there are limited studies addressing its toxicological effects, particularly concerning cardiovascular toxicity. In the present study, zebrafish embryos acutely exposed to 2-hATQ (0, 0.4, 0.8, and 1.2 mg/L) at different concentrations showed significant cardiac developmental abnormalities, as evidenced by slowing of the heart rate, pericardial edema, and the cardiac malformations. Transcriptomic sequencing analysis and quantitative real-time polymerase chain reaction (qPCR) study showed that 2-hATQ induced cardiac ferroptosis via disruption of the antioxidant system, glutathione depletion, increase in polyunsaturated fats and accumulation of iron ions. Co-exposure of ferroptosis inhibitor (Fer-1) effectively rescues the morphological abnormalities of the heart. After exposing adult zebrafish to low concentration 2-hATQ for 28 days, ventricular morphology was reduced and internal structures showed partial vacuolation and fibrosis. This study reveals for the first time the negative influence of 2-hATQ exposure on the cardiac development of aquatic organisms and this discovery may offer significant insights and references for an in-depth understanding of 2-hATQ and other ANT-related environmental risk substances.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"286 ","pages":"Article 107485"},"PeriodicalIF":4.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic study of bisphenol A on the growth and microcystin production of Microcystis aeruginosa at different nitrogen levels revealed by the metabolomic analysis","authors":"Meng Yang ,&nbsp;Daolin Du ,&nbsp;Yonglai Xue ,&nbsp;Zhicong Dai ,&nbsp;Xiangrong Wang","doi":"10.1016/j.aquatox.2025.107488","DOIUrl":"10.1016/j.aquatox.2025.107488","url":null,"abstract":"<div><div>Water safety problems caused by cyanobacterial blooms, resulting from excessive inputs of anthropogenic pollutants, nitrogen (N) and phosphorus nutrients, have become a significant environmental issue. Limited knowledge exists regarding the mechanisms regulating the toxic by-products synthesized by cyanobacterial cells, especially the hepatotoxin microcystins (MCs). Here, the regulatory mechanisms of bisphenol A (BPA) on the growth and MCs production of <em>Microcystis aeruginosa</em> at different N levels were investigated using metabolomic analysis. The results indicated that the number of cyanobacterial cells decreased by 9.34 % and 14.36 % when exposed to 1 µM and 10 µM BPA, respectively, and oxidative damage was also observed in <em>M. aeruginosa</em> at low N levels. Increased N levels weakened the inhibitory effects of BPA on growth, chlorophyll a (Chl-a) synthesis, and photosynthetic activity, but significantly stimulated the release of extracellular MCs from <em>M. aeruginosa</em> cells, resulting in up to 22.1 % more MCs released. The up-regulated expression of metabolites associated with the Calvin cycle and glycolysis/gluconeogenesis metabolism promoted the cyanobacterial cell growth and photosynthesis under the combined stress of BPA and N. Fluctuating expression of metabolites related to the amino acid metabolism and the TCA cycle mediated <em>M. aeruginosa</em>’s MCs production and release, as well as N assimilation. These results suggested that the long-term coexistence of exogenous endocrine disruptors and MCs-producing cyanobacteria poses a potential threat to the environmental and ecological security of lake waters.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107488"},"PeriodicalIF":4.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent effects of urea fertilizers on the green toad (Bufotes sitibundus): From growth enhancement to lethal toxicity","authors":"Alireza Pesarakloo,&nbsp;Masoumeh Najibzadeh,&nbsp;Zahra Zarei","doi":"10.1016/j.aquatox.2025.107486","DOIUrl":"10.1016/j.aquatox.2025.107486","url":null,"abstract":"<div><div>This study elucidates urea fertilizers' dual, concentration-dependent effects on the green toad (<em>Bufotes sitibundus</em>, Pallas, 1771), revealing a nonlinear dose–response pattern that may reflect a potential hormetic effect at low doses versus acute toxicity at elevated concentrations. Larvae exposed to urea gradients (5–50 mg/L and 5–7 g/L) were evaluated for survival, metamorphic dynamics, and growth indices. At 10 mg/L, larvae exhibited optimal outcomes: 95 % survival (57/60 metamorphosed), accelerated metamorphosis (50 days), and superior morphometrics (mean length: 30.72 mm; post-metamorphic weight: 0.56 g), possibly consistent with hormetic stimulation. Conversely, high concentrations (50 mg/L) induced 71 % mortality (44 deaths), stunted growth (28 mm; 0.41 g), and oxidative stress, while 5–7 g/L caused complete embryonic lethality within 6 days. Intermediate doses (15–20 mg/L) showed reduced mortality (78.3 %–65 % survival) but delayed development, reflecting resource-stress trade-offs. Statistical analyses (Kaplan-Meier, ANOVA, Tukey HSD) confirmed significant dose-response disparities (<em>p</em> &lt; 0.05). These findings underscore the critical balance between agricultural productivity and ecological conservation, advocating for stringent regulation of fertilizer use in amphibian habitats to mitigate biodiversity loss. The study highlights the necessity of context-specific thresholds in ecotoxicological risk assessments, emphasizing that sublethal doses may mask long-term ecological impacts through hormesis-driven adaptation.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"286 ","pages":"Article 107486"},"PeriodicalIF":4.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of legacy and emerging antimicrobial compounds to early-life stages of rainbow trout (Oncorhynchus mykiss)","authors":"Mawuli Amekor ,&nbsp;Evan Kohlman ,&nbsp;Catherine Roberts ,&nbsp;Alper James Alcaraz ,&nbsp;Niteesh Jain ,&nbsp;Ahmad Al-Dissi ,&nbsp;Markus Hecker ,&nbsp;Markus Brinkmann ,&nbsp;Natacha Hogan","doi":"10.1016/j.aquatox.2025.107487","DOIUrl":"10.1016/j.aquatox.2025.107487","url":null,"abstract":"<div><div>Antimicrobial compounds enter freshwater systems via municipal wastewater, potentially affecting aquatic life. While the toxicity of triclosan (TCS), a legacy antimicrobial, is well-documented, less is known about emerging alternatives such as chloroxylenol (PCMX) and methylisothiazolinone (MIT). This study evaluated the developmental and molecular effects of these compounds on early-life stage rainbow trout (Oncorhynchus mykiss). Embryos were exposed to nominal concentrations of 0.39–400 µg/L from hatch to 28 days post-hatch (dph). Mortality and sublethal endpoints (edema, spinal curvature, jaw deformities, swim-up time) were assessed, and transcriptomic responses were measured at 96 h using the EcoToxChip RT-qPCR platform. TCS and PCMX reduced survivability, with 28-d LC50 values of 107 and 254 µg/L, respectively. TCS increased jaw deformities and edema, while PCMX induced spinal deformities and edema at ≥241 µg/L. MIT had no observable effects on survival or development. No significant changes were detected in swim-up time or histopathology of gill, liver, or intestine across treatments. Transcriptomic analysis revealed 55, 25, and 3 differentially expressed genes (DEGs) in response to TCS, PCMX, and MIT, respectively. TCS and PCMX shared regulation of 19 genes linked to metabolic, endocrine, and reproductive pathways, suggesting similar modes of action. These findings indicate that TCS and PCMX exert low but distinct sublethal and molecular toxicity, while MIT showed minimal effects. The EcoToxChip effectively detected early transcriptomic responses, supporting its application in rapid chemical hazard assessment of both legacy and emerging antimicrobials.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"286 ","pages":"Article 107487"},"PeriodicalIF":4.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chemicals on mobility in the bivalve mollusc Dreissena polymorpha","authors":"Nicolas Berthelot ,&nbsp;Mélissa Palos Ladeiro ,&nbsp;Mathilde Orquevaux ,&nbsp;Bénédicte Chaperon ,&nbsp;Cécile Pochet ,&nbsp;Alain Geffard","doi":"10.1016/j.aquatox.2025.107482","DOIUrl":"10.1016/j.aquatox.2025.107482","url":null,"abstract":"<div><div>The freshwater bivalve <em>Dreissena polymorpha</em> is largely used in ecotoxicology for applications including biomonitoring, based on tools including measurement of sub-individual effects (<em>i.e.</em> biomarkers). Technological advances and the increasing occurrence of neuroactive compounds in the environment have led to studies increasingly focusing on the behaviour of aquatic species in ecotoxicology. This could provide potentially sensitive and/or early individual behavioural responses as toxicity indicators. This study aimed to investigate the effects of seven chemicals on the mobility of mussels after dislodgement, using continuous motion tracking (<em>i.e.</em> video tracking). Several metrics were used to describe mobility, based on motility and metrics for distance travelled. Mussels exposed to fluoxetine were particularly more likely to move significantly sooner and over larger distances. Other chemicals may induce non-linear responses with bell-shaped patterns. Behaviour could expand the range of biological responses studied, often on a sub-individual level, in ecotoxicology. More in-depth study of behavioural endpoints in <em>Dreissena polymorpha</em> appears promising, as this could provide potentially sensitive and/or early individual responses integrating physiological endpoints up to highest level.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"286 ","pages":"Article 107482"},"PeriodicalIF":4.1,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disrupted senses and social cues: Impacts of nanoplastics and methylmercury on zebrafish neurodevelopment","authors":"Mathilde J.L. Oger,&nbsp;Jean-Baptiste Robert,&nbsp;Patrick Kestemont,&nbsp;Valérie Cornet","doi":"10.1016/j.aquatox.2025.107483","DOIUrl":"10.1016/j.aquatox.2025.107483","url":null,"abstract":"<div><div>With the advancement of analytical techniques and the global increase in plastic pollution, nanoplastics (NPs) have been increasingly detected across all ecosystems and in deep tissues, including the human brain. Notably, NPs are capable of acting as vectors for co-contaminants such as heavy metals, raising concerns about their combined toxicity. In this study, zebrafish (<em>Danio rerio</em>) were exposed to 250 nm polystyrene NPs (1000 µg/L), either alone or in combination with 10 µg/L of methylmercury (MeHg), during the first 30 days of development. Neuromast development and vibrational startle response were evaluated at both larval and juvenile stages, while brain histopathology and social behaviour were assessed at the juvenile stage. NPs were found to accumulate in neuromasts during early larval stages, temporarily impairing their development and affecting the startle response to vibrational stimuli. NPs alone also altered social behaviour, enhancing body contact frequency and duration, yet resulting in looser shoal cohesion. Co-exposure with MeHg led to more severe behavioural impairments, including diminished social interactions, reduced responses to stress, and delayed neuromast development. Despite these functional changes, histopathological examination revealed minimal structural alterations in the central nervous system, except for significant accumulation of glial cells in the optic tectum following NPs-exposure. These findings suggest that behavioural disruptions are likely mediated by impaired peripheral sensory inputs and altered neurotransmission rather than apparent neuroanatomical damages.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"286 ","pages":"Article 107483"},"PeriodicalIF":4.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of an in vitro bioassay using C6/36 insect cells as a model for evaluating toxicity of aquatic contaminants to invertebrates","authors":"Matthew Johnson ,&nbsp;Kimberly A. Finlayson ,&nbsp;Jason P. van de Merwe ,&nbsp;Frederic D.L. Leusch","doi":"10.1016/j.aquatox.2025.107481","DOIUrl":"10.1016/j.aquatox.2025.107481","url":null,"abstract":"<div><div>Aquatic invertebrates play an important role in ecosystem functioning and are commonly used for <em>in vivo</em> toxicity assessments of environmental contaminants. However, <em>in vitro</em> bioassays employing invertebrate cell lines remain underutilised, despite their potential to improve toxicity testing and reduce reliance on whole-organism assays. This study aimed to develop and validate a suite of <em>in vitro</em> bioassays using the Asian tiger mosquito (<em>Aedes albopictus</em>) larval cell line (C6/36) to assess the cytotoxicity, oxidative stress, and genotoxicity of 24 common environmental contaminants. Cytotoxic effects were observed in 71 % of tested chemicals, while 25 % induced oxidative stress, and 63 % showed measurable genotoxicity. A strong correlation (R² = 0.83) between <em>in vitro</em> cytotoxicity data and acute toxicity data previously reported for <em>Daphnia</em> spp. suggests that C6/36 cells may serve as a reliable surrogate for traditional <em>in vivo</em> invertebrate toxicity tests. These findings support the broader application of invertebrate cell lines for environmental risk assessment, offering a refined approach for evaluating the toxicological properties of aquatic contaminants, while potentially reducing reliance on animal testing.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"286 ","pages":"Article 107481"},"PeriodicalIF":4.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}