Aquatic Toxicology最新文献

{"title":"Effects of polypropylene microparticles ingestion on the growth and physiology of Piaractus brachypomus, and its accumulation and tissue damage","authors":"Camila Gomes de Oliveira ,&nbsp;Túlio Pacheco Boaventura ,&nbsp;Fabio Aremil Costa dos Santos ,&nbsp;André de Sena Souza ,&nbsp;Sidney dos Santos Silva ,&nbsp;Camila Silva Brey Gil ,&nbsp;Ronald Kennedy Luz ,&nbsp;Rodrigo Lambert Oréfice","doi":"10.1016/j.aquatox.2025.107338","DOIUrl":"10.1016/j.aquatox.2025.107338","url":null,"abstract":"<div><div>The presence of microplastics in aquatic environments is a growing concern due to their impacts on fish health. This study aimed to investigate the effects of different concentrations of polypropylene microparticles (PPMs) added to the diet of juvenile <em>Piaractus brachypomus</em>, on their growth, survival, physiology and tissue health. A total of 280 juveniles weighing 13.45 ± 2.07 g were divided into five treatments (0, 10, 100, 1000 and 5000 mg PPMs per kg of feed) with four replicates each. The juveniles were cultivated for 60 days in recirculating aquaculture systems. Biometrics and blood and tissue collections were performed after 10 and 60 days of the experiment. Juvenile performance was affected by the presence of PPMs in the diet within 10 days, with a reduction in final weight for the different concentrations. No differences were observed in juvenile growth after 60 days compared to the control. Total protein, hematocrit, and the enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) changed significantly for the juveniles of the different treatments in both periods. No changes were observed in liver tissue histology; however, the presence of PPMs and damage to microvilli were verified in intestinal tissue. Although PPMs were observed in the intestine of juveniles in both periods, there was no migration of PPMs to the muscle. The addition of PPMs to juvenile diet caused growth disturbances within 10 days and blood and histological alterations in the intesine, without resulting in severe consequences leading to mortality in both periods.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107338"},"PeriodicalIF":4.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-biomarker analysis of sub-chronic PAH mixture effects in fish at environmentally relevant levels","authors":"B. Matos ,&nbsp;I. Bramatti ,&nbsp;C.D. Santos ,&nbsp;V. Branco ,&nbsp;M. Martins","doi":"10.1016/j.aquatox.2025.107350","DOIUrl":"10.1016/j.aquatox.2025.107350","url":null,"abstract":"<div><div>Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants in aquatic ecosystems, occurring as complex mixtures with unpredictable toxicity. Although PAHs are procarcinogenic, their harmful effects require metabolic activation, leading to reactive metabolites and reactive oxygen species (ROS) that can cause DNA damage.</div><div>This study assessed the toxic effects of individual PAHs (Phenanthrene and Benzo[a]pyrene) and their mixtures (1:2 and 2:1 ratios) on juvenile seabream (<em>Sparus aurata)</em> after 42 days of exposure at 0.2nmol.L⁻¹ . Biomarkers related to oxidative stress, detoxification, and lipid peroxidation were analysed in the liver and gills (e.g., glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST), catalase (CAT), lipoperoxidation (LPO). Liver gene expression (Cytochromes P450 (CYP1A), GST3, tumour protein p53 (TP53) and blood cell DNA damage were also studied. Correlation analyses and Non-Metric Multidimensional Scaling (NMDS) were used to relate treatments and biomarkers.</div><div>Results suggested differences in organ responses, with the gills generally showing the most significant changes in GSH levels, GST activity, and LPO compared to the control group. DNA repair mechanisms appeared to prevent significant genotoxicity as assessed by the comet assay. However, erythrocytic nuclear anomalies (ENAs) were notably higher in fish exposed to Phe, B[a]P, and the 2:1 B[a]P:Phe mixture compared to the control group. Interestingly, the 2:1 Phe:B[a]P mixture appeared to have an enhanced effect, showing a marked upregulation of GST3 mRNA (up to 7-fold), possibly influenced by the higher proportion of Phe. MDS analysis proved to be a valuable tool in identifying patterns among biological responses, offering insight into how fish cope with PAH exposure and helping to uncover the unpredictable effects of chemical mixtures.</div><div>This study highlights the need for further research into the interactions of PAH mixtures, employing multi-analysis approach and underscores the importance of revising environmental guidelines to account for their effects.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107350"},"PeriodicalIF":4.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of MWCNTs induced hepatotoxicity in Cyprinus carpio: New perspective from a Lipidomic study","authors":"Xiaochan Gao,&nbsp;Jiaqi Shen,&nbsp;Pinpin Lv,&nbsp;Xuehan Niu,&nbsp;Yong Huang","doi":"10.1016/j.aquatox.2025.107339","DOIUrl":"10.1016/j.aquatox.2025.107339","url":null,"abstract":"<div><div>Carbon nanotubes (CNTs), an extensively applied nanomaterial, can cause damage to the liver in fish. However, the underlying lipotoxic modes of CNTs exposure have not been studied. In the present study, juvenile <em>Cyprinus carpio</em> was exposed via water to various doses of multi-walled CNTs (MWCNTs) (0, 0.25, and 2.5 mg <em>L</em>⁻¹) for a consecutive 28-day period. Our results showed elevated hepatosomatic index (HSI) and aspartate aminotransferase (AST) activities in plasma in all exposure groups, indicating that MWCNTs exposure induced liver dysfunction. Significantly increased low-density lipoprotein (LDL) and total cholesterol (CHO) levels along with significantly decreased triglyceride (TG) levels were observed in fish of HSC (2.5 mg <em>L</em>⁻¹ MWCNTs), indicating abnormal lipid metabolism. Subsequently, the livers of fish in the HSC group were harvested for LC-MS/MS-based targeted lipidomic analysis. Changes in the lipid profiles showed 87 and 92 lipids significant up-regulated and down-regulated, respectively. These significantly altered lipids were classed into 20 lipid classes. Among them, Cer, Hex1Cer, CerG2GNAc1, SM, SPH, and phSM belong to sphingolipid. Taken together with our previous findings, these results suggested that the dysregulation of sphingolipid metabolism may be associated with oxidative stress and apoptosis induced by MWCNTs exposure in <em>C. carpio</em>. This study provides new insights into the potential mechanism of MWCNTs in fish, especially from the aspect of lipid metabolism.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107339"},"PeriodicalIF":4.1,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating cell surface extraction methods for improved assessment of silver nanoparticle bioaccumulation","authors":"Arin Kantarciyan ,&nbsp;Inés Segovia-Campos ,&nbsp;Vera I. Slaveykova","doi":"10.1016/j.aquatox.2025.107340","DOIUrl":"10.1016/j.aquatox.2025.107340","url":null,"abstract":"<div><div>Methods to differentiate surface-bound and internalized Ag in phytoplankton are essential for understanding bioaccumulation but remain limited, especially for studies involving silver nanoparticles (AgNPs). To address this, we assessed biologically relevant ligands for extracting adsorbed Ag from <em>Chlamydomonas reinhardtii</em> and <em>Cyclotella meneghiniana</em> exposed to Ag⁺ or AgNPs, focusing on ligand type, concentration, and extraction time. Additionally, we examined the impact of these agents on AgNP stability and microalgal membrane integrity. The ligands <span>l</span>-histidine, <span>l</span>-glutamine, <span>l</span>-cysteine, and <span>d</span>-penicillamine were tested. The thiol-containing <span>d</span>-penicillamine, tested for the first time, showed the highest efficiency in desorbing Ag⁺ from Ag⁺-treated cells, followed by <span>l</span>-cysteine. Optimal conditions were 1 mmol L⁻¹ ligand concentration and 5 min contact time. For AgNP-exposed cells, two sequential cell resuspensions in Ag-free medium were sufficient to detach loosely bound AgNPs. Then, hydrogen peroxide (H₂O₂), a biologically relevant oxidant, was introduced for the first time in the extraction procedure to facilitate the oxidative dissolution of nanoparticles. A combination of 0.5 mmol L^-1 H₂O₂ with a 1 mmol L^-1 <span>l</span>-cysteine or <span>d</span>-penicillamine effectively removed Ag from the cell walls of <em>C. meneghiniana</em> and <em>C. reinhardtii</em>. The tested extraction agents did not affect unexposed phytoplankton cells. However, AgNP-exposed cells treated with H₂O₂ showed slightly increased cell membrane damage in diatoms. A dissolution of AgNP was found in the presence of <span>l</span>-histidine and <span>d</span>-penicillamine and H₂O₂. These findings highlight the need to develop species-specific washing methodologies in AgNPs bioaccumulation studies and provide insight into optimized extraction methods for assessing AgNP adsorption, internalization, and toxicity.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107340"},"PeriodicalIF":4.1,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PBK-TD modelling of the gonadotropic axis: Case study with two azole fungicides in female zebrafish","authors":"Tu-Ky Ly ,&nbsp;Edith Chadili ,&nbsp;Olivier Palluel ,&nbsp;Karyn Le Menach ,&nbsp;Hélène Budzinski ,&nbsp;Cleo Tebby ,&nbsp;Nathalie Hinfray ,&nbsp;Rémy Beaudouin","doi":"10.1016/j.aquatox.2025.107337","DOIUrl":"10.1016/j.aquatox.2025.107337","url":null,"abstract":"<div><div>Endocrine disruptors (EDs) can disrupt the gonadotropic axis, which consists of the Hypothalamus-Pituitary-Gonads (HPG), notably by altering aromatase (cyp19a), a key enzyme regulating the endocrine system and reproductive function in fish. The effects of EDs can be predicted by integrating both toxicokinetic (TK) and toxicodynamic (TD) processes in order to relate adverse outcomes to external exposures. In this study, we developed a physiologically based kinetic-toxicodynamic model to simulate the disruption of the HPG axis (PBK-TD, hereafter named PBK-HPG) in female zebrafish exposed to either of two aromatase inhibitors, imazalil or prochloraz. The model was calibrated using Bayesian methods and supported by novel experimental data, including measurements of vitellogenin, 17β-estradiol, and 11-ketotestosterone levels, along with in vivo monitoring of the cyp19a1a gene in transgenic cyp19a1a-GFP ebrafish. Seamless integration of a PBK model within a TD model of the HPG-axis, provided the link between external exposure and internal levels of imazalil and prochloraz in key organs, allowing for mechanistic predictions of their inhibitory effects on gonadal aromatase. Our PBK-HPG model accurately predicted both baseline homeostasis and the effects of aromatase inhibition, with all endocrine endpoints including reproductive disruption, i.e., decreased egg production, falling within a twofold range of both experimental and literature data. Therefore, our PBK-HPG model could further support the development of a mechanistic qAOP with TK considerations. The model offers significant potential for improving environmental risk assessments of EDs and possibly other stressors across species.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107337"},"PeriodicalIF":4.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of black phosphorus nanosheet exposure on growth, reproduction, antioxidant mechanisms, and transcriptomic responses in Daphnia magna","authors":"Tianlie Luo ,&nbsp;Ping Zhang ,&nbsp;Jinyu Wang ,&nbsp;Jingjing Shi ,&nbsp;Yubo Di ,&nbsp;Guo Liu ,&nbsp;Willie J.G.M. Peijnenburg","doi":"10.1016/j.aquatox.2025.107333","DOIUrl":"10.1016/j.aquatox.2025.107333","url":null,"abstract":"<div><div>Black phosphorus nanosheets (BPNS), a novel two-dimensional nanomaterial, have garnered significant attention in biomedical and technological applications due to their exceptional physicochemical properties. However, their widespread use raises concerns about potential environmental risks. In this study, we elucidate the toxicological mechanisms of BPNS on <em>Daphnia magna</em> (<em>D. magna</em>), a model aquatic organism. The results reveal that BPNS is efficiently absorbed and accumulates in the intestinal tract of <em>D. magna</em>. Exposure to low concentrations of BPNS significantly alters developmental and reproductive performance, as evidenced by a 2-day acceleration in the time to first brood and an increase in body length from 3.1 to 3.3 mm. Furthermore, BPNS exposure induces oxidative stress in <em>D. magna</em>, characterized by elevated reactive oxygen species (ROS) levels, enhanced activities of superoxide dismutase (SOD) and catalase (CAT), and increased malondialdehyde (MDA) concentrations. RNA sequencing analysis indicates that dysregulation of iron homeostasis plays a pivotal role in mediating oxidative stress in <em>D. magna.</em> Concurrently, detoxification mechanisms are activated, as evidenced by upregulation of genes associated with chitin and carbohydrate metabolism, as well as cuticle structure components. Additionally, BPNS exposure modulates key signaling pathways, including the lysosomal pathway, starch and sucrose metabolism, and steroid biosynthesis, which collectively enhance the stress tolerance of <em>D. magna</em>. These findings provide critical insights into the ecological implications of BPNS release into aquatic ecosystems, highlighting the need for comprehensive risk assessments of emerging nanomaterials.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107333"},"PeriodicalIF":4.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dioxin and PCB levels in sea trout with ulcerative disease syndrome","authors":"Malgorzata Warenik-Bany ,&nbsp;Szczepan Mikolajczyk ,&nbsp;Marek Pajurek ,&nbsp;Pawel Malagocki ,&nbsp;Sebastian Maszewski ,&nbsp;Michał Reichert","doi":"10.1016/j.aquatox.2025.107336","DOIUrl":"10.1016/j.aquatox.2025.107336","url":null,"abstract":"<div><div>This paper reports concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) in fish with symptoms of ulcerative disease syndrome (UDS) and in healthy fish collected from the Słupia River in northwestern Poland. Altogether 174 samples from sea trout were analyzed using an isotope dilution technique with high-resolution gas chromatography coupled with high-resolution mass spectrometry. The levels determined in material collected from healthy and diseased fish did not raise concerns for human health. All results were below the maximum permitted levels established in European Commission Regulation No 2023/915. However, considering instead fish health, dioxins and PCBs could have</div><div>a negative effect at the levels detected. The highest concentration of dioxins and dioxin-like PCBs (DL- PCBs) was found in diseased sea trout muscles and was 6.19 pg World Health Organization Toxic Equivalents (WHO-TEQ) <em>g</em>⁻¹ fresh weight (f.w.). The most-accumulated dioxin congeners were 2,3,7,8-tetrachlorinated dibenzofuran (TCDF) and 2,3,4,7,8-pentachlorinated dibenzofuran (PeCDF). The most abundant DL-PCBs were PCB-118 and PCB-105, and among non-dioxin like PCBs they were PCB-153, PCB-138, and PCB-101. The polluted sea trout environment and exposure to a mixture of chemical pollutants may increase the incidence of disease. The presence of dioxins and PCBs in their bodies may disrupt the functioning of their immune system. Also the time of spawning, when the fish are exhausted, may affect the increase in the incidence of UDS</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107336"},"PeriodicalIF":4.1,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel insights into copper-induced Chinese mitten crab hepatopancreas mitochondrial toxicity: Oxidative stress, apoptosis and BNIP3L-mediated mitophagy","authors":"Xinping Guo ,&nbsp;Minghao Shen ,&nbsp;Su Jiang ,&nbsp;Xiumei Xing ,&nbsp;Cong Zhang ,&nbsp;Shaowu Yin ,&nbsp;Kai Zhang","doi":"10.1016/j.aquatox.2025.107335","DOIUrl":"10.1016/j.aquatox.2025.107335","url":null,"abstract":"<div><div>Copper (Cu) is an important metal pollutant commonly found in aquatic environment. Cu-based nanoparticles (NPs) have been increasingly fabricated, and led to cytotoxicity in aquatic animals. Herein, the mechanisms underlying the CuSO₄/Cu-NPs-mediated perturbation of the hepatopancreatic mitochondrial function at different concentrations were investigated and compared. After exposing <em>Eriocheir sinensis</em> to 0 (control), 5, 50, and 500 μg/L CuSO₄ and 10 μg/L Cu-NPs for 21 days, hepatopancreases were retrieved. The results revealed that Cu-NPs or excess CuSO₄ induced ultrastructural damage following a time-dose effect, as indicated by swelling and degeneration of the lumen of hepatic tubules. Cu-NPs or excess CuSO₄ exposure decreased the antioxidative capacity and led to the over-accumulation of reactive oxygen species (ROS). Moreover, the mitochondrial membrane potential (MMP) was reduced and apoptosis induced. Additionally, both CuSO₄ and Cu-NPs increased the numbers of mitophagosomes and the mRNA and protein levels of microtubule associated protein 1 light chain 3 beta (LC3B), and triggered mitophagy through BCL2 interacting protein 3 like (BNIP3L)/Beclin1 pathway. Altogether, this study provides a basis for exploring Cu-mediated potential mitochondrial autophagy activation mechanisms, uncovered the difference between CuSO₄ and Cu-NPs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107335"},"PeriodicalIF":4.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive toxicity and parental transmission effects of 4-methylbenzylidene camphor (4-MBC) exposure in adult zebrafish","authors":"Xiaofeng Wu,&nbsp;Chenyu Zhou,&nbsp;Jing Wang,&nbsp;Mengxi Cao,&nbsp;Ling Wang,&nbsp;Yong Liang","doi":"10.1016/j.aquatox.2025.107334","DOIUrl":"10.1016/j.aquatox.2025.107334","url":null,"abstract":"<div><div>4-methylbenzylidene camphor (4-MBC), a commonly used UV absorber, is frequently detected in aquatic environment. So far the reproductive toxicity of parental 4-MBC exposure and its effects on gonadal development in offsprings are not clear. In the present study, male and female adult F0 zebrafish were exposed to 100 nM 4-MBC for 14 consecutive days. Our data showed that 4-MBC exposure resulted in gonadal damage in the parental gonads and decreased egg production in females and sperm viability in males. In addition, exposure to 4-MBC resulted in increased levels of estradiol (E2), follicle stimulating hormone (FSH), and luteinizing hormone (LH) in females and decreased testosterone (T) in males, suggesting the estrogenic and antiandrogenic effects of 4-MBC. Parental 4-MBC exposure did not change the hatchability and mortality of the F1 generation, but caused significantly decreased heart rate and gonadal developmental retardation in 60 dpf fish by interfering with the HPG axis. Therefore, 4-MBC exposure to adult zebrafish caused gonadal damage and reduced reproductive performance in the parental generation, which was sex-dependent and caused intergenerational toxicity to the F1 generation. The present study provides new insights into the ecological risks of 4-MBC and its potential contribution to adverse reproductive outcomes in humans.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107334"},"PeriodicalIF":4.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population-specific responses of Lemna minor to silver nanoparticle exposure: Implications for standardizing toxicity assessments","authors":"Qingqing Wang ,&nbsp;Shasha Wei ,&nbsp;Daomin Cheng ,&nbsp;Longyi Yuan ,&nbsp;Wei Li ,&nbsp;Hong Sheng Jiang","doi":"10.1016/j.aquatox.2025.107332","DOIUrl":"10.1016/j.aquatox.2025.107332","url":null,"abstract":"<div><div>The globally distributed and excellent growth properties of <em>Lemna minor</em> make it an ideal model species in ecotoxicology. However, the variability among different L. <em>minor</em> populations is often overlooked in laboratory toxicity assessments, which could lead to inaccurate toxicity evaluations, especially for newly emerging pollutants. In this study, we investigated the responses of L. <em>minor</em> populations from various regions (Wuhan (WH), South Korea (KR), Yunnan (YN), and Tibet (TB)) to silver nanoparticles (AgNPs), a newly emerging pollutant, at concentrations ranging from 0 to 10 mg <em>L</em>⁻¹ over a 72-hour exposure period. The results showed a significant increase in silver accumulation in L. <em>minor</em> tissues with increasing AgNPs concentration. Concurrently, photosynthetic pigments content (chlorophyll a, b, and carotenoids) and chlorophyll fluorescence parameters exhibited a dose-dependent decline, while malondialdehyde levels increased, indicating that AgNPs induced oxidative stress in different L. <em>minor</em> populations. Notably, the populations displayed significant differences in tolerance to AgNPs: the KR population showed the highest tolerance, followed by TB, while the YN and WH populations were more sensitive. Further analysis revealed that the differences in toxicity response among L. <em>minor</em> populations were mainly attributed to variations in Ag accumulation capacity. Therefore, it is recommended that, when using L. <em>minor</em> from different regions to assess AgNPs toxicity, parameters could be standardized based on the silver accumulated by the plants rather than the externally applied silver. This approach will improve the comparability of results across laboratories and provide a more accurate understanding of AgNPs toxicity in global aquatic ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107332"},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}