Aquatic Toxicology最新文献

{"title":"Toxicological effects of azadirachtin on aquatic species: A review of its role in biopesticides","authors":"Daniele Sacco ,&nbsp;Josef Velíšek ,&nbsp;Nikola Mikušková","doi":"10.1016/j.aquatox.2025.107547","DOIUrl":"10.1016/j.aquatox.2025.107547","url":null,"abstract":"<div><div>Azadirachtin (AZA) is a bioactive compound extracted from the neem tree <em>Azadirachta indica</em>. It is commonly used in biopesticide formulations due to its antifeedant, growth-inhibiting, and reproductive-disrupting properties. While AZA is considered an environmentally friendly alternative to synthetic pesticides because it degrades rapidly in soil and is compatible with organic farming and aquaculture, there are growing concerns about its ecotoxicological effects on aquatic ecosystems. Therefore, this review critically examines the acute and chronic toxicity to non-target aquatic organisms. The review presents studies on the biomolecular, behavioural, and physiological toxicity effects of AZA on aquatic species, providing a comprehensive overview of its impact. Studies on acute and chronic exposure reveal significant lethal and sublethal effects on various species, including fish and invertebrates. Additionally, uncertainties regarding the behaviour of AZA metabolites and AZA formulations raise further concerns about potential long-term environmental impacts. This is particularly important in aquatic environments where AZA may persist and affect non-target species. Despite its biodegradability, AZA ability to disrupt aquatic ecosystems highlights the need for further research on its environmental fate, the effects of its degradation by-products, and the long-term risks associated with its widespread use. Addressing these knowledge gaps is essential for ensuring AZA-based pesticides are safe and sustainable for application in integrated pest management (IPM) programs.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"288 ","pages":"Article 107547"},"PeriodicalIF":4.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144900388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmentally relevant levels of sulfamethoxazole (SMX) alter the skin and gastrointestinal microbiome of adult male and female zebrafish (Danio rerio)","authors":"Nazish Iftikhar ,&nbsp;Isaac Konig ,&nbsp;Elizabeth Brammer-Robbins ,&nbsp;Marianne Kozuch ,&nbsp;Joseph H. Bisesi Jr. ,&nbsp;Imran Hashmi ,&nbsp;Christopher J. Martyniuk","doi":"10.1016/j.aquatox.2025.107546","DOIUrl":"10.1016/j.aquatox.2025.107546","url":null,"abstract":"<div><div>This study determined the effects of sulfamethoxazole (SMX) exposure on the gastrointestinal and skin microbiome of male and female adult zebrafish (<em>Danio rerio</em>). Adult male and female zebrafish were exposed to measured, environmentally relevant concentrations of 0, 0.75, and 304.9 µg/L SMX for 21 days. Bacterial DNA was isolated from feces and skin for 16S sequencing using NextGen Illumina MiSeq. The taxonomic data were analyzed for differences in abundance and diversity between sexes and treatment groups. There were no differences in beta diversity in the fecal and skin microbiota between males and females. However, there was a statistically significant difference between the control and low exposure concentrations of SMX for fecal samples (<em>p</em> &lt; 0.05). The genera <em>Paracoccus, Nubsella, Chryseobacterium, Delftia</em>, and <em>Exiguobacterium</em> decreased in relative abundance on the skin, while an elevated abundance of <em>Cetobacterium</em> was detected in the low-treatment group. For the fecal samples, the low concentration of SMX increased the relative abundance of <em>Microtrichaceae</em> and <em>Rhizobiaceae</em>, and both concentrations decreased the abundance of <em>Staphylococcaceae</em>. Differences between males and females in the abundance of genera were detected for the fecal (e.g., <em>Unclassified env.OPS_17</em>), and skin microbiome (e.g., <em>Runella, Cupriavidus, Lysobacter, Psychroglaciecola, Plesiomonas</em>). Microbial pathways related to energy metabolism were also predicted to be altered following SMX exposure in both the fecal and skin microbiomes. This study provides essential information concerning the detrimental impact of sulfonamides on the skin and digestive system of fish and improves understanding of the possible hazards posed by antibiotic pollution to aquatic organisms.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"288 ","pages":"Article 107546"},"PeriodicalIF":4.3,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144900478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol attenuates ethylene glycol butyl ether-impaired zebrafish fertility via antioxidant mechanism","authors":"Zheyuan Chen,&nbsp;Han Zhang,&nbsp;Lulu Han,&nbsp;Bo Zhao,&nbsp;Shanshan Zhao,&nbsp;Zhangjie Chu,&nbsp;Yang Gao,&nbsp;Haidong Li,&nbsp;Xiaoxin Dai","doi":"10.1016/j.aquatox.2025.107545","DOIUrl":"10.1016/j.aquatox.2025.107545","url":null,"abstract":"<div><div>Industrial pollutants and their metabolite derivatives constitute formidable threats to aquatic ecosystems upon environmental discharge. Ethylene glycol butyl ether (EGBE), an extensively employed organic solvent prevalent in industrial and household products, exhibits well-characterized reproductive toxicity. This investigation evaluated the protective effect of resveratrol (RSV) against EGBE-induced reproductive toxicity in zebrafish ovaries. Findings revealed that subsequent to EGBE (200 mg/L for 20 days), both fertilization rate (from 83.39 % ± 2.37 % to 55.70 % ± 1.91 %, <em>p</em> &lt; 0.05) and hatching rate (from 91.06 % ± 3.69 % to 48.87 % ± 2.25 %, <em>p</em> &lt; 0.05) manifested significant decrements. Transcriptomic analysis unveiled dysregulation of oxidative stress-associated genes, whereas functional enrichment analysis demonstrated differential gene expression clustering within the lysosome, Notch signaling pathway, Apelin signaling pathway, and biosynthesis of unsaturated fatty acids pathways—all fundamental to oxidative stress regulation. EGBE concurrently compromised the endogenous antioxidant system through modulation of superoxide dismutase (SOD) and catalase (CAT) enzymatic activities, concomitant with perturbations in glutathione (GSH) and malondialdehyde (MDA) levels. Finally, RSV restored ovarian SOD and CAT activities functionality, augmented GSH concentration, attenuated MDA concentration, increased the fertilization rate (from 55.70 % ± 1.91 % to 88.27 % ± 1.17 %, <em>p</em> &lt; 0.05) and hatching rate (from 48.87 % ± 2.25 % to 93.06 % ± 1.66 %, <em>p</em> &lt; 0.05). In summary, our research data substantiate that RSV ameliorates reproductive toxicity in zebrafish caused by EGBE. Nevertheless, comprehensive investigation remains requisite to elucidate EGBE’s chronic ecological ramifications and multi-trophic impacts.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107545"},"PeriodicalIF":4.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver nanoparticles aggravated clothianidin-induced toxicity toward Limnodrilus hoffmeisteri","authors":"Shengjin Zhang ,&nbsp;Mengting Wu ,&nbsp;Xiao Wu ,&nbsp;Yayun Yu ,&nbsp;Hua Fang ,&nbsp;Yunlong Yu ,&nbsp;Luqing Zhang","doi":"10.1016/j.aquatox.2025.107543","DOIUrl":"10.1016/j.aquatox.2025.107543","url":null,"abstract":"<div><div>The extensive application of silver nanoparticles (AgNPs) has resulted in their significant release into aquatic environments, leading to a high likelihood of co-occurrence with other contaminants. Among these, pesticides represent one of the most prevalent groups in aquatic systems. However, the combined effects of AgNPs and pesticides on aquatic organisms, especially benthic species, remain poorly understood. This study comparatively assessed the joint toxicity of AgNPs and clothianidin (CLO), a widely used neonicotinoid insecticide, on <em>Limnodrilus hoffmeisteri</em> under 24-h acute exposure in a water-only system and 14-d subacute exposure in a sediment-water system. The results demonstrated that AgNPs and CLO demonstrated synergistic lethality toward <em>L. hoffmeisteri</em> under acute exposure, partially attributed to CLO-facilitated dissolution of AgNPs and exacerbated oxidative stress upon co-exposure. Transcriptomic analysis further revealed that CLO exposure upregulated the genes associated with cellular respiration and protein synthesis/degradation; however, these adaptive responses were suppressed by the presence of AgNPs, thereby weakening the worms’ tolerance to CLO. Under subacute exposure, AgNPs alone exhibited negligible toxicity but enhanced CLO bioaccumulation in the worms, resulting in intensified oxidative stress and ultimately aggravating CLO-induced growth inhibition and neurotoxicity in <em>L. hoffmeisteri</em>. These results collectively revealed that AgNPs could synergistically amplify the toxicity of CLO toward <em>L. hoffmeisteri</em> in both water-only and water-sediment systems. The findings of this work contribute to a comprehensive understanding of the ecological risks associated with the co-occurrence of AgNPs and pesticides.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107543"},"PeriodicalIF":4.3,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicological assessment of pesticide mixtures: effects of imidacloprid and propiconazole on Danio rerio and Astyanax lacustris","authors":"Maria Vitória Raupp Sebastião ,&nbsp;Johana Marcela Concha Obando ,&nbsp;Davi da Silva Nascimento ,&nbsp;Angela Hisamitsu Carolino ,&nbsp;Gisele de Souza Silva ,&nbsp;Beatriz Pellis ,&nbsp;Nicole Grasmuk Israel ,&nbsp;Eduardo Alves de Almeida ,&nbsp;Carlos Augusto Prata Gaona ,&nbsp;Guilherme Wolff Bueno ,&nbsp;Levi Pompermayer Machado ,&nbsp;Ana Leticia Madeira Sanches","doi":"10.1016/j.aquatox.2025.107544","DOIUrl":"10.1016/j.aquatox.2025.107544","url":null,"abstract":"<div><div>Despite global efforts to promote sustainable agriculture, the increasing use of pesticides remains a critical concern, particularly in local ecosystems with limited environmental monitoring. This study aimed to evaluate the toxicological effects of the insecticide @Provado (imidacloprid) and the fungicide @Tilt (propiconazole) on two freshwater fish species, <em>Danio rerio</em> (zebrafish) and <em>Astyanax lacustris</em> (lambari). Standard ecotoxicological parameters (EC₅₀, LC₅₀, EC₁₀, NOEC, and LOEC) were determined for each pesticide individually and in combination to assess toxicity and potential additive, synergistic, or antagonistic interactions. Sublethal exposures were conducted to evaluate morphological alterations in <em>D. rerio</em> larvae and biochemical responses in adult <em>A. lacustris</em>, focusing on oxidative stress biomarkers (glutathione-S-transferase-GST, catalase-CAT, malondialdehyde-MDA) in gill and liver tissues. For <em>A. lacustris</em>, LC₅₀ values were 1.1 mg L⁻¹ for Tilt and 5.49 mg L⁻¹ for Provado, with the mixture exhibiting LC₅₀ of 0.35 + 13.7 mg L⁻¹. For <em>D. rerio</em>, the EC₅₀ for Tilt was 1.62 mg L⁻¹ and the EC₁₀ was 0.32 mg L⁻¹. Acute exposure resulted in reduced larval survival and malformations, including yolk sac and pericardial edema. GST activity significantly decreased in gills following exposure to Tilt (6.0 µg L⁻¹) and Provado (1 mg L⁻¹), whereas liver GST activity increased under Tilt exposure. These results demonstrate the significant toxic impacts of these pesticides, emphasizing environmental risks and the complexity of their interactions in aquatic ecosystems, especially where regulatory control is insufficient.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107544"},"PeriodicalIF":4.3,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aged nanoplastics reprogram the ER stress-autophagy crosstalk: A mechanistic gateway to skeletal malformations in zebrafish","authors":"Sihong Long,&nbsp;Xiaojie Wen,&nbsp;Manni Dai,&nbsp;Yufan Pan,&nbsp;Wanjing Liu,&nbsp;Chunhua Zhan","doi":"10.1016/j.aquatox.2025.107542","DOIUrl":"10.1016/j.aquatox.2025.107542","url":null,"abstract":"<div><div>Micro(nano)plastics (MNPs) are pervasive in aquatic environments, with aged MNPs being the predominant form due to environmental weathering. However, their developmental toxicity, particularly regarding skeletal formation, remains poorly understood. In this study, aged polystyrene nanoplastics (PSNPs) were prepared via 48-hour UV irradiation and compared with pristine PSNPs in zebrafish embryo exposures starting within 4 h post-fertilization (hpf) and lasting for 7 days. Both pristine and aged PSNPs induced skeletal malformations, but aged PSNPs caused more severe effects, including decreased hatching rates, increased mortality, and altered larval body length. Histopathological analysis revealed disrupted muscle fiber organization and lipid accumulation in the yolk sac. Elevated reactive oxygen species (ROS) and calcium ion levels were observed in both treatment groups. Aged PSNPs significantly upregulated GRP78 and RAB7 protein expression, increased transcription of the Endoplasmic Reticulum stress (ER stress) marker <em>eif2α</em>, and downregulated key genes involved in bone development (<em>bmp2b, bmp4</em>) and autophagy (<em>atg13, atg5</em>). These findings indicate that aged PSNPs exacerbate oxidative stress, activate ER stress pathways, inhibit autophagy, and impair skeletal development. This study highlights the heightened developmental toxicity of environmentally aged MNPs and underscores the necessity of including aged plastic particles in ecological risk assessments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107542"},"PeriodicalIF":4.3,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between micro(nano)plastics and natural organic matter: implications for toxicity mitigation in aquatic species","authors":"Naima Hamid ,&nbsp;Marriya Sultan ,&nbsp;Muhammad Junaid ,&nbsp;Stuart Cairns ,&nbsp;Iain Robertson ,&nbsp;Houda Javed ,&nbsp;De-Sheng Pei","doi":"10.1016/j.aquatox.2025.107541","DOIUrl":"10.1016/j.aquatox.2025.107541","url":null,"abstract":"<div><div>Plastics have significantly contributed to modern conveniences owing to their ease of use, stability, and adaptability. However, the fragmentation of plastics into microplastics (MPs) and nanoplastics (NPs) poses significant environmental risks. These micro(nano)plastics (MNPs) can adsorb various pollutants and pathogens, potentially posing significant ecological risks. This review critically examines the natural organic matter (NOM) in mitigating the toxicity of MNPs in both marine and freshwater species. Evidence suggests that NOM facilitates the formation of an eco-corona (EC) on MNPs, thereby reducing toxicity. Reduced toxicity attributed to EC formation has been observed in various freshwater species, such as <em>Danio rerio</em> and <em>Daphnia magna</em>, as well as marine species, including sea urchins, European sea bass, and marine algae. The presence of natural organic matter (NOM), particularly fulvic acid (FA) and humic acid (HA), significantly mitigates the toxic effects of MNPs, with HA exhibiting a strong protective effect. The interactions between MNPs and NOM, including the formation of the EC, which encompasses a protein corona component, are pivotal in understanding toxicity mitigation in aquatic environments. This review highlights the need for further research to elucidate the interactions between MNPs and NOM, and their role in mitigating toxicity across marine and freshwater ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107541"},"PeriodicalIF":4.3,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the role of environmentally realistic concentrations of titanium dioxide nanoparticles in altering gametogenesis and gonadal health of female Pacific oysters (Magallana gigas): a histopathological approach","authors":"F. Fernández-García ,&nbsp;M. Martins ,&nbsp;E. Raymond ,&nbsp;A. Carvalhais ,&nbsp;I.B. Oliveira ,&nbsp;J.F. Asturiano ,&nbsp;M. Pacheco ,&nbsp;C. Mieiro","doi":"10.1016/j.aquatox.2025.107539","DOIUrl":"10.1016/j.aquatox.2025.107539","url":null,"abstract":"<div><div>Titanium dioxide nanoparticles (TiO₂ NPs) raise environmental concerns due to their potential adverse effects on marine bivalves. However, research on females, particularly their gonad quality and reproduction, remains limited. Despite the availability of numerous health status biomarkers, histopathological analysis remains crucial for assessing toxicological effects. This study aimed to shed light on the potential reprotoxicity of environmentally realistic concentrations of TiO₂ NPs during gametogenesis in female <em>Magallana gigas</em>. Oysters were exposed to waterborne 10 and 100 μg/L of TiO₂ NPs for 3 and 7 days. Thereafter, females were identified, and the gonadal health status was assessed through qualitative and quantitative histology alongside titanium bioaccumulation evaluation. Partial indices for interlobular and intralobular alterations were applied for the first time to the gonad, improving the understanding of histopathological alterations caused by TiO₂ NPs. Our results indicated that the severity of histopathological alterations was concentration and time-dependent, even though no bioaccumulation was detected. We observed an acceleration of gametogenic stage development and disturbances in sex determination. This study provides tools to assess TiO2 NPs’ impacts on bivalve populations and ecosystem balance, especially in highly touristic marine areas.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107539"},"PeriodicalIF":4.3,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced toxicity of bisphenol F compared to its predecessor bisphenol A in rainbow trout (Oncorhynchus mykiss) during a six-week feeding trial","authors":"Vendula Stoklasova ,&nbsp;Premysl Mikula ,&nbsp;Aneta Hollerova ,&nbsp;Petr Marsalek ,&nbsp;Nikola Peskova ,&nbsp;Ales Franc ,&nbsp;Lucie Sedlackova ,&nbsp;Frantisek Tichy ,&nbsp;Eva Postulkova ,&nbsp;Jan Mares ,&nbsp;Zdenka Svobodova ,&nbsp;Jana Blahova","doi":"10.1016/j.aquatox.2025.107540","DOIUrl":"10.1016/j.aquatox.2025.107540","url":null,"abstract":"<div><div>This study aimed to assess the toxicity of bisphenol A (BPA) and bisphenol F (BPF) in rainbow trout following six weeks of dietary exposure. Fish were exposed to BPA, BPF, or their combination at nominal concentrations of 10 and 1000 µg/kg (i.e., BPA_low, BPA_high, BPF_low, BPF_high, BPA+BPF_low). Haematological analysis revealed a significant reduction (<em>p</em> &lt; 0.05) in leukocytes and lymphocytes, but only in the BPF_high group. Plasma biochemical markers showed significant increases (<em>p</em> &lt; 0.05) in creatinine in BPA_low and elevated amylase activity in both BPF groups. Surprisingly, markers of endocrine disruption, such as vitellogenin and thyroxine, were significantly elevated (<em>p</em> &lt; 0.05) only in BPF_high and BPA+BPF_low, despite no pathological changes being observed in the gonads. Oxidative stress markers were significantly affected, with increased catalase activity (<em>p</em> &lt; 0.05) in the liver, kidney, and gill across all groups. Additionally, plasma DNA damage was significantly (<em>p</em> &lt; 0.05) reduced in BPA_low and BPF_high. In contrast, significant elevations (<em>p</em> &lt; 0.05) of ceruloplasmin and ferric reducing/antioxidant power were observed in the BPA+BPF_low and both BPF groups, respectively. Histological examination revealed liver congestion and dystrophy in both BPA groups, hyaline droplet degeneration in the tubular epithelial cells of the caudal kidney across all exposed groups, and deformation of gill filaments in BPA+BPF_low and both BPA groups. Our findings underscore that the increasing use of BPA analogues may pose a greater risk than the original compound in certain contexts, emphasizing the need for further studies to understand the long-term effects of bisphenol analogues, particularly at environmentally relevant concentrations and in mixtures.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107540"},"PeriodicalIF":4.3,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BPA disrupts crustacean reproduction: Tissue-specific accumulation, oxidative stress, and gene dysregulation in Macrobrachium nipponense","authors":"Jintao Liu ,&nbsp;Ruijie Zhu ,&nbsp;Shuhao Wang ,&nbsp;Xinyuan Dai ,&nbsp;Anran Zou ,&nbsp;Fengjuan Jiang ,&nbsp;Shengming Sun ,&nbsp;Jiangtao Ou ,&nbsp;Weihong Zhao","doi":"10.1016/j.aquatox.2025.107538","DOIUrl":"10.1016/j.aquatox.2025.107538","url":null,"abstract":"<div><div>Bisphenol A (BPA), a pervasive environmental endocrine disruptor, increasingly threatens aquatic ecosystems due to its reproductive toxicity. This study investigates how BPA disrupts ovarian development in <em>Macrobrachium nipponense</em> - by analyzing its tissue-specific bioaccumulation (ovaries, hepatopancreas, fertilized eggs), antioxidant responses, and developmental gene regulation. Experimental results demonstrated that after 10-day exposure to 10, 100, and 1000 μg/L BPA, significant BPA accumulation was observed in both ovaries and hepatopancreas, while fertilized eggs showed notable accumulation only at the highest concentration (1000 μg/L) (<em>P</em> &lt; 0.05). The ovarian glutathione peroxidase (GSH-Px) activity displayed a concentration-dependent increase, contrasting with the inverse pattern observed in fertilized eggs. Gene expression analysis revealed that cytochrome P450 49A1 (<em>CYP49a1</em>) in both ovaries and hepatopancreas reached peak levels at the highest BPA concentration, whereas cathepsin D (<em>CTSD</em>) and catalase (<em>CAT</em>) expression peaked at the intermediate concentration (100 μg/L) in both tissues (<em>P</em> &lt; 0.05), while female sterile homeotic (<em>fsh</em>) in ovaries remained steady but significantly decreased in hepatopancreas upon BPA exposure (<em>P</em> &lt; 0.05). These results provide preliminary evidence that BPA may disrupt ovarian development in <em>M. nipponense</em> through interfering with antioxidant systems and altering the expression of key developmental genes, highlighting its potential endocrine-disrupting effects in this commercially important crustacean species.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107538"},"PeriodicalIF":4.3,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144830276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}