Aquatic Toxicology最新文献

{"title":"Molecular docking analysis and in vivo assessment of zinc oxide nanoparticle toxicity in zebrafish larvae","authors":"Valbona Aliko ,&nbsp;Ledia Vasjari ,&nbsp;Erman S. Istifli ,&nbsp;Grejsi Gjonaj ,&nbsp;Federica Impellitteri ,&nbsp;Caterina Faggio ,&nbsp;Elisabetta Benedetti ,&nbsp;Silvana Zugaro ,&nbsp;Annamaria Iannetta ,&nbsp;Monia Perugini","doi":"10.1016/j.aquatox.2024.107112","DOIUrl":"10.1016/j.aquatox.2024.107112","url":null,"abstract":"<div><div>The zinc oxide nanoparticles (ZnO-NPs) being widely employed in several industries and consumer products, are raising concerns about their safety on aquatic biota and human health. This study aims to investigate the possible toxicological effects of ZnO-NPs through a combined <em>in vivo</em> and <em>in silico</em> approach. Zebrafish embryos were exposed to several ZnO-NPs concentrations and morphological alterations and lipid peroxidation (MDA) were investigated. Furthermore, molecular docking simulations were applied to study the intermolecular interactions of ZnO-NPs against critical embryonic proteins namely zebrafish hatching enzyme1 (ZHE1) as well as the superoxide dismutase (SOD1). Treatment with ZnO-NPs resulted in an increase in MDA concentration and a decrease in antioxidant enzyme levels. Besides a significant decrease in mRNA expression of key enzymes of ROS detoxification genes, a modulation of inflammatory genes with a low downregulation of <em>tnf-α,</em> and an upregulation of <em>il-1β</em> were observed. Docking study suggests that the delayed hatching and increased cellular oxidative stress in zebrafish embryos may occur through a synergistic mechanism based on the ZnO-NP—dependent inhibition of ZHE1 and SOD1 enzymes. The integration of <em>in vivo</em> assessments with <em>in silico</em> computational modeling provided a more comprehensive evaluation of potential physiological risks in zebrafish embryos associated with nanomaterial exposure.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107112"},"PeriodicalIF":4.1,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-environmental responses of Eichhornia crassipes rhizobacteria community to co-stress of per(poly)fluoroalkyl substances and microplastics","authors":"Zi-Wei Chen ,&nbsp;Zu-Lin Hua","doi":"10.1016/j.aquatox.2024.107109","DOIUrl":"10.1016/j.aquatox.2024.107109","url":null,"abstract":"<div><div>The stabilization of rhizobacteria communities plays a crucial role in sustaining healthy macrophyte growth. In light of increasing evidence of combined pollution from microplastics (MPs) and per- and polyfluoroalkyl substances (PFASs), Selecting typical floating macrophyte as a case, this study explored their impacts using hydroponic simulations and 16S rRNA high-throughput sequencing. A total of 31 phyla, 77 classes, 172 orders, 237 families, 332 genera, and 125 rhizobacteria species were identified. <em>Proteobacteria</em> (16.19% to 57.70%) was the dominant phylum, followed by <em>Bacteroidota</em> (12.34% to 44.48%) and <em>Firmicutes</em> (11.31% to 36.36%). In terms of α-diversity, polystyrene (PS) MPs and PFASs significantly impacted community abundance (ACE and PD-tree) rather than evenness (Shannon and Pielou) compared to the control. βMNTD and βNTI analyses revealed that PS MPs enhanced deterministic assembly processes driven by F-53B and GenX, while mitigating those induced by PFOA and PFOS. Contamination treatments narrowed the ecological niche breadths at both the phylum (5% (PS) to 49.91% (PS &amp; PFOA)) and genus levels (8% (PS) to 63.96% (PS &amp; PFOA)). Functionally, MPs and PFASs decreased the anaerobic capacity and ammonia nitrogen utilization of rhizosphere bacteria. This study enhances our understanding of the microecological responses of macrophyte-associated bacteria to combined MP and PFAS contamination and offers insights into ecological restoration strategies and mitigating associated environmental risks.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107109"},"PeriodicalIF":4.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of diethyl phthalate on freshwater planarian behaviour, regeneration, and antioxidant defence","authors":"Chandani R. Verma ,&nbsp;Tushar Khare ,&nbsp;Paromita Chakraborty ,&nbsp;Sachin M. Gosavi ,&nbsp;Miloslav Petrtýl ,&nbsp;Lukáš Kalous ,&nbsp;Pradeep Kumkar","doi":"10.1016/j.aquatox.2024.107110","DOIUrl":"10.1016/j.aquatox.2024.107110","url":null,"abstract":"<div><div>Diethyl phthalate (DEP) has been widely used as a plasticiser in various consumer products, including cosmetics, personal care items, and pharmaceuticals, and recent studies reported a higher abundance of this priority phthalate in the aquatic environment. DEP is a potential endocrine disruptor, affecting immune systems in humans and wildlife even at low-level chronic exposure. As concern over phthalates increases globally, regulatory bodies focus more on their environmental impact. However, limited research is available, particularly using model organisms like planarians. Planarians are ideal for toxicological studies and may provide insightful information on pollutants' neurotoxic, developmental, and ecological effects, especially in freshwater environments where planarians play a vital role in ecosystem balance. Therefore, the objective of the current study was to examine the toxicity of DEP using the freshwater <em>Dugesia</em> sp.<em>,</em> as an experimental animal. The LC₅₀ for the test organism was calculated using DEP concentrations of 800, 400, 200, 100, and 50 µM, with an estimated LC₅₀ of 357.24 µM. Furthermore, planarians were exposed to sub-lethal DEP concentration (178.62 µM) for one day as well as eight days to evaluate the impact of DEP on planarian locomotion, feeding behaviour, and regeneration ability. At sub-lethal concentration, locomotion and feeding ability were decreased, and regeneration was delayed. Furthermore, neuro-transmittance in planaria was altered by sub-lethal DEP concentration, as indicated by a reduced acetylcholinesterase (AChE) activity. DEP exposure induced oxidative damage in the tested planarians as shown by a marked increase in stress biomarkers, including lipid peroxidation levels and antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), and glutathione S-transferase (GST). Our study revealed that DEP exposure may prove fatal to freshwater organisms, such as planarians. The observed alterations in behaviour and regeneration ability demonstrate the severity of the effects exerted by DEP as a toxicant in aquatic ecosystems, thereby indicating the need to restrict its usage to protect aquatic environments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107110"},"PeriodicalIF":4.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethyl butyrate inhibits caudal fin regeneration in adult zebrafish by disrupting extracellular matrix remodeling","authors":"Sijie Zhang ,&nbsp;Hao Wang ,&nbsp;Yunlong Meng ,&nbsp;Mijia Li ,&nbsp;Yang Li ,&nbsp;Xinhao Ye ,&nbsp;Shiyi Duan ,&nbsp;Shimei Xiao ,&nbsp;Huiqiang Lu ,&nbsp;Keyuan Zhong","doi":"10.1016/j.aquatox.2024.107111","DOIUrl":"10.1016/j.aquatox.2024.107111","url":null,"abstract":"<div><div>Wound healing and tissue regeneration are influenced by a variety of factors. Adverse lifestyle habits, such as excessive alcohol consumption, delay wound healing and increase the risk of secondary infections. Ethyl butyrate is a common food additive widely used to enhance the aroma of alcoholic beverages. This additive is generally considered harmless to human health in both industrial and domestic settings. However, the ecotoxicity and its effects on wound healing have not been elucidated. In this study, we used zebrafish as the experimental animal, and the caudal fins were amputated to explore the effects of ethyl butyrate on wound healing and tissue regeneration. The effect of ethyl butyrate on blastema and bone regeneration and its impact on the transcriptional levels of regeneration-related genes and inflammation-related genes were evaluated. RNA-seq was conducted to determine the differentially expressed genes (DEGs) between the treatment and the control groups. KEGG and GO analysis was conducted to explore the functions of DEGs. Significantly enriched GO terms and KEGG pathways were identified to explore the molecular mechanism underlying the inhibition of zebrafish caudal fin regeneration by ethyl butyrate. The results demonstrated that ethyl butyrate significantly inhibited the regeneration of zebrafish caudal fins, including blastema and bone regeneration. Ethyl butyrate exposure significantly downregulated the expression of genes associated with bone and blastema regeneration and inflammation response. KEGG and GO functional analyses revealed that the DEGs were associated with significant enrichment of extracellular matrix-receptor interactions. Ethyl butyrate treatment downregulated the expression of most extracellular matrix-related genes. These findings indicate that ethyl butyrate potentially modulates pathways associated with the structure, adhesion, modification, and degradation of the extracellular matrix, thereby disrupting extracellular matrix remodeling, inhibiting wound inflammation, impairing blastema and bone regeneration and ultimately hindering caudal fin regeneration. In summary, the findings demonstrate that ethyl butyrate disrupts extracellular matrix remodeling and inhibits the regeneration of zebrafish caudal fins. These results provide valuable insights into the rational use of ethyl butyrate and further investigation of wound healing mechanisms.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107111"},"PeriodicalIF":4.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible mechanisms for adverse effects on zebrafish sperm and testes associated with low-level chronic PFOA exposure","authors":"Yuhan Wang ,&nbsp;Yu Huo ,&nbsp;Afrasyab Khan,&nbsp;Ningna Ma,&nbsp;Weijun Mai","doi":"10.1016/j.aquatox.2024.107108","DOIUrl":"10.1016/j.aquatox.2024.107108","url":null,"abstract":"<div><div>Perfluorooctanoic acid (PFOA), which is widely used during the manufacturing of fluoropolymer coatings and polytetrafluoroethylene, is now a widespread pollutant in the environment and within the human body. This study used zebrafish, an aquatic model species, to investigate how low levels of chronic PFOA exposure affect the reproductive system. The results of the experiments in which zebrafish were exposed to 414 ng/L or 4140 ng/L for 60 days showed a variety of adverse effects on testicular tissue and sperm, including dose-dependent changes in plasma estradiol and testosterone levels, various sperm malformations, decreased sperm motility and concentration, and PFOA-induced oxidative stress and testicular damage with increased rates of apoptosis. In addition, offspring of the zebrafish that had been exposed to PFOA were characterized by increased malformation and mortality. Subsequent transcriptional analyses of the male gonads revealed the significant activation of oxidative stress bioprocesses and immuno-inflammatory signaling pathways, along with the dysregulation of reproductive bioprocesses. In conclusion, low-level chronic exposure to PFOA affects both the reproductive performance of adults and the development of offspring; the mechanisms for these adverse effects involve alterations in several molecular pathways that may be involved in PFOA-induced oxidative stress and reproductive abnormalities. The presented data can be used to assess the ecotoxicity of PFOA to the male reproductive system at environmentally-relevant concentrations.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107108"},"PeriodicalIF":4.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A proof-of-concept multi-tiered Bayesian approach for the integration of physiochemical properties and toxicokinetic time-course data for Daphnia magna","authors":"Jacob-Joe Collins ,&nbsp;Joe Reynolds ,&nbsp;Bruno Campos ,&nbsp;Patrik Engi ,&nbsp;Claudia Rivetti ,&nbsp;Tymoteusz Pietrenko ,&nbsp;Mark R. Viant ,&nbsp;George Fitton","doi":"10.1016/j.aquatox.2024.107107","DOIUrl":"10.1016/j.aquatox.2024.107107","url":null,"abstract":"<div><div>The use of <em>in silico</em> and <em>in vitro</em> methods, commonly referred to as New Approach Methodologies (NAMs), has been proposed to support environmental (and human) chemical safety decisions, ensuring enhanced environmental protection. Toxicokinetic models developed for environmentally relevant species are fundamental to the deployment of a NAMs-based safety strategy, enabling the conversion between external and internal chemical concentrations, although they require historical toxicokinetic data and robust physical models to be considered a viable solution. <em>Daphnia magna</em> is a key model organism in ecotoxicology albeit with limited and scattered quantitative toxicokinetic data, as for most invertebrates, resulting in a lack of robust toxicokinetic models. Moreover, current <em>D. magna</em> models are chemical specific, which restricts their applicability domain. One aim of this study was to address the current data availability limitations by collecting toxicokinetic time-course data for <em>D. magna</em> covering a broad chemical space and assessing the dataset's uniqueness. The collated toxicokinetic dataset included 48 time-courses for 30 chemicals from 17 studies, which was developed into an R package named <em>AquaTK</em>, with 11 studies unique to our work when compared to existing databases. Subsequently, a proof-of-concept Bayesian analysis was developed to estimate the steady-state concentration ratio (internal concentration / external concentration) from the data at three different levels of precision given three different data availability scenarios for environmental risk assessment. Specifically, an atrazine case study illustrates the multi-level modelling approach providing improvements (uncertainty reductions) in predictions of ratios for increasing amounts of data availability. Our work provides a consistent and self-contained Bayesian framework that irrespective of the hierarchy or resolution of individual experiments, can utilise the available information to generate optimal predictions of steady-state concentration ratios in <em>D. magna</em>. This approach is paramount to supporting the implementation of a NAMs based environmental safety paradigm shift in environmental risk assessment.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107107"},"PeriodicalIF":4.1,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective role of melatonin against radiation-induced disruptions in behavior rhythm of zebrafish (danio rerio)","authors":"Weichao Zhao ,&nbsp;Jing Yao ,&nbsp;Yu Liu,&nbsp;Liang Mao,&nbsp;Chuqi He,&nbsp;Dingxin Long","doi":"10.1016/j.aquatox.2024.107106","DOIUrl":"10.1016/j.aquatox.2024.107106","url":null,"abstract":"<div><div>Ionizing radiation, as an increasingly serious environmental pollutant, has aroused widespread public concern. Melatonin, as an indole heterocyclic compound, is known to have anti-inflammatory and antioxidant effects. However, few studies have considered the comprehensive impact of melatonin on radiation damage. In this study, we used zebrafish as experimental materials and employed methods such as acridine orange staining, enzyme-linked immunosorbent assay (ELISA), video tracking for automated behavior analysis, microscope imaging, and real-time fluorescence quantitative analysis. Zebrafish embryos at 2 h post-fertilization (hpf) were treated under four different experimental conditions to assess their growth, development, and metabolic consequences. Our findings indicate that 0.10 Gy gamma radiation significantly augments body length, eye area, spine width, and tail fin length in zebrafish, along with a marked increase in oxidative stress (<em>P</em> &lt; 0.05). Moreover, it enhances cumulative swimming distance, time, and average speed, suggesting elevated activity levels. We observed circadian rhythm phase shifts, peak increases, and cycle shortening, accompanied by abnormal expression of genes pivotal to biological rhythms, exercise, melatonin synthesis, apoptosis/anti-apoptosis, and oxidation/antioxidant balance. The inclusion of melatonin (1 × 10^-5 mol/L MLT) ameliorated these radiation-induced anomalies, while its independent effect on zebrafish was negligible. Melatonin can regulate oxidative stress responses, hinders apoptosis responses, and reprogramming the expression of rhythm-related genes in zebrafish embryos after reprogramming radiation stimulation. Overall, our research highlights melatonin's critical role in countering the biological damage inflicted by gamma radiation, proposing its potential as a therapeutic agent in radiation protection.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107106"},"PeriodicalIF":4.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The long-term effects of norgestrel on the reproductive and thyroid systems in adult zebrafish at environmentally relevant concentrations","authors":"Shuyan Mai ,&nbsp;Yan-Qiu Liang ,&nbsp;Shuhui Zhou ,&nbsp;Hongjie Lin ,&nbsp;Zhongdian Dong ,&nbsp;Chang-Gui Pan ,&nbsp;Qingwei Kong ,&nbsp;Shaoshuai Wang ,&nbsp;Shiqing Wang ,&nbsp;Zhong Lin ,&nbsp;Liping Hou","doi":"10.1016/j.aquatox.2024.107105","DOIUrl":"10.1016/j.aquatox.2024.107105","url":null,"abstract":"<div><div>Progestins are crucial steroid hormones that have attracted wide attention due to their endocrine disrupting effects in fish. The aim of this study is to investigate the effects of long-term exposure to low concentrations of norgestrel (NGT) on the reproductive and thyroid endocrine systems of adult zebrafish. Adult zebrafish were exposed to 7 and 39 ng/L NGT for a duration of 90 days. The results revealed that exposure to 39 ng/L NGT led to a significant up-regulation of 3β-hydroxysteroid dehydrogenase (<em>hsd3b</em>) and 20β-hydroxysteroid dehydrogenase (<em>hsd20b</em>) genes in the ovary of female zebrafish. Additionally, there was a significant up-regulation of 11β-hydroxysteroid dehydrogenase 2 (<em>hsd11b2</em>) gene in the testis of male zebrafish. Furthermore, egg production decreased significantly, accompanied by notable alterations in the proportion of ovarian development stages, as well as reductions of sex hormone levels (E2, 11-KT, and T) in both females and males. However, long-term exposure to low concentrations of NGT did not lead to changes in thyroid hormone levels and thyroid histopathology in adult zebrafish. The overall results imply that environmental concentrations of NGT have a strong endocrine disrupting effect on the reproductive system of zebrafish, while the thyroid system is not sensitive to NGT exposure. The present study underscores the reproductive endocrine impacts of NGT and emphasizes the necessity for prolonged exposure at environmental concentrations.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107105"},"PeriodicalIF":4.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fate and effects of an environmentally relevant mixture of microplastics in simple freshwater microcosms","authors":"Sara Martínez-Pérez ,&nbsp;Theresa Schell ,&nbsp;Daniel Franco ,&nbsp;Roberto Rosal ,&nbsp;Paula E. Redondo-Hasselerharm ,&nbsp;Virtudes Martínez-Hernández ,&nbsp;Andreu Rico","doi":"10.1016/j.aquatox.2024.107104","DOIUrl":"10.1016/j.aquatox.2024.107104","url":null,"abstract":"<div><div>Most studies assessing the effects of microplastics (MPs) on freshwater ecosystems use reference materials of a certain size, shape, and polymer type. However, in the environment, aquatic organisms are exposed to a mixture of different polymers with different sizes and shapes, resulting in different bioaccessible fractions and effects. This study assesses the fate and effects of an environmentally relevant mixture of high-density polyethylene (HDPE) fragments, polypropylene (PP) fragments, and polyester (PES) fibres in indoor freshwater microcosms over 28 days. The MP mixture contained common polymers found in freshwater ecosystems, had a size range between 50 and 3887 µm, and was artificially aged using a mercury lamp. The invertebrate species included in the microcosms, <em>Lymnea stagnalis</em> (snail) and <em>Lumbriculus variegatus</em> (worm)<em>,</em> were exposed to four MP concentrations: 0.01, 0.05, 0.1 and 1 % of sediment dry weight. MPs fate was assessed by performing a balance of the MPs in the surface water, water column, and sediment after a stabilization period and at the end of the experiment. Sedimentation rates per day were calculated (2.13 % for PES, 1.46 % for HDPE, 1.87 % for PP). The maximum size of MPs taken up by the two species was determined and compared to the added mixture and their mouth size. The size range taken up by <em>L. variegatus</em> was smaller than <em>L. stagnalis</em> and significantly different from the size range in the added mixture. The No Observed Effect Concentrations (NOECs) for the reproduction factor of <em>L. variegatus</em> and the number of egg clutches produced by <em>L. stagnalis</em> were 0.01 % and 0.1 % sediment dry weight, respectively. The EC10 and EC50 for the same endpoint for <em>L. stagnalis</em> were 0.25 % and 0.52 %, respectively<em>.</em> This study shows that current MP exposure levels in freshwater sediments can result in sub-lethal effects on aquatic organisms, highlighting the importance of testing MP mixtures.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107104"},"PeriodicalIF":4.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0166445X24002741/pdfft?md5=54a4c58bbdfa04a5ba83a32740156666&pid=1-s2.0-S0166445X24002741-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evaluations of oxidative stress and neurotoxicity in threatened endemic fish Barbus meridionalis from Osor River (Spain)","authors":"Gülüzar Atli ,&nbsp;Lluis Zamora ,&nbsp;Anna Vila-Gispert ,&nbsp;Helena Guasch","doi":"10.1016/j.aquatox.2024.107099","DOIUrl":"10.1016/j.aquatox.2024.107099","url":null,"abstract":"<div><div>Mediterranean barbel (<em>Barbus meridionalis</em>) an endemic species is currently facing habitat destruction and pollution in Osor River (Spain) due to mining runoff that has severely deteriorated the water quality by metals, primarily zinc (Zn). In order to assess the potential risk of metal contamination and hydrological changes in the Osor River by using oxidative stress and neurotoxicity biomarkers via IBR analyses in the barbel, five different stations were chosen: upstream (S1: reference site and S2: hydrologically changed), mine (S3), and downstream (S4 and S5). The highest tissue metal levels were measured particularly at S3 and following downstream sites. SOD activity and the GSH system parameters (GPX, GST, and GSH) were the most sensitive oxidative stress indicators among the antioxidant system parameters. The organs with the greatest changes in antioxidant biomarkers were the liver and gill. As a sign of neurotoxicity, AChE activities significantly raised in the brain and muscle but drastically lowered in the kidney, liver, and gill particularly in the area of mine and downstream compared to reference site. Integrated biomarker response index (IBR) method was applied to visualize the affect of metal and hydrological alterations with biomarker response according to sites in the Osor River. IBR analyzes together with correlations between metal levels and oxidative stress biomarkers, emphasized that S2 and S3 have the greatest impact on the biomarker levels due to mine activity and hydrological changes highlighting the vulnerability to extinction of native fish <em>B. meridionalis</em>. It is also critical to assess the current data based on the multi-biomarker approach for a range of detrimental effects on fish fitness at the individual level as well as population persistence from an ecological standpoint.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107099"},"PeriodicalIF":4.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}