Aquatic Toxicology最新文献

{"title":"Biochemical and cytogenetic consequences of settleable atmospheric particulate matter on Pacific white shrimp Litopenaeus vannamei","authors":"F. Copetti ,&nbsp;C.R. Nobre ,&nbsp;M.S. Paço ,&nbsp;T.F.T. de Camargo ,&nbsp;B.B. Moreno ,&nbsp;M.N. Fernandes ,&nbsp;R. Schveitzer ,&nbsp;C.D.S. Pereira","doi":"10.1016/j.aquatox.2025.107322","DOIUrl":"10.1016/j.aquatox.2025.107322","url":null,"abstract":"<div><div>This study hypothesizes that micro and nanoparticles of metals from metallurgical atmospheric emissions can trigger sublethal effects on the Pacific white shrimp <em>Litopenaeus vannamei</em>. We aimed to analyze cytotoxicity (Lysosomal Membrane Stability - LMS), Lipid peroxidation (LPO), genotoxicity (DNA strand break), and neurotoxicity (Acetylcholinesterase activity AChE) in shrimp exposed to environmentally relevant concentrations (0.001, 0.1, and 1.0 g.L⁻¹) of settleable atmospheric particulate matter (SePM) for different times (T2, T4, T7, T15 and T30 days), and in several tissues (gills, hemolymph, muscle,e and hepatopancreas). LPO within the first 2 day, and LMS showed significant differences. From the seventh to the fifteenth day of exposure, the concentration of 0.1 g.L⁻¹ exhibited significant effects. In the most extended exposure period (30 days), all concentrations triggered cytotoxicity effects on the Pacific white shrimp <em>Litopenaeus vannamei.</em> Thus, exposure to SePM can impair essential cellular functions, denoting a pre-pathological status. These findings underscore the potential hazards of metallurgical SePM to estuarine and farmed shrimp populations, emphasizing the need for ongoing monitoring and effective mitigation strategies to ensure aquaculture sustainability.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107322"},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyethylene microplastics perforate the chorion defense, triggering developmental cardiotoxicity at zebrafish","authors":"Yejin Kim ,&nbsp;Hyerin Lee ,&nbsp;Yun Hak Kim ,&nbsp;Chang-Kyu Oh","doi":"10.1016/j.aquatox.2025.107331","DOIUrl":"10.1016/j.aquatox.2025.107331","url":null,"abstract":"<div><div>Polyethylene microplastics are pervasive environmental pollutants that pose potential risks to aquatic organisms. This study investigates the effects of polyethylene microplastics on zebrafish (<em>Danio rerio,</em> Hamilton 1822) embryo development, with a focus on whether the chorion, a protective barrier surrounding the embryo, is effective in blocking polyethylene penetration. Contrary to previous findings that suggested the chorion could prevent larger microplastics (&gt;0.7 µm) from entering, our study demonstrates that polyethylene particles sized 1–4 µm can still negatively impact embryo development without dechorionation. Embryos were exposed to polyethylene at concentrations of 0.01, 0.1, and 1 mg l^-1, followed by RNA sequencing to assess gene expression changes. Despite no significant differences in survival, hatching, or body length between control and treated groups, a significant reduction in heart rate was observed at higher concentrations, indicating potential sub-lethal cardiotoxicity. Further, RT-qPCR validation confirmed significant downregulation of key heart development-related genes, particularly fbln1 and fn1b, in polyethylene-exposed embryos. These findings highlight the ability of polyethylene microplastics to penetrate natural barriers such as the chorion and induce physiological and developmental changes. Our results emphasize the need for further research into the long-term effects of microplastic exposure on aquatic ecosystems.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107331"},"PeriodicalIF":4.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological effects, bioaccumulation, and metabolic pathways of tricresyl phosphate in Scenedesmus obliquus","authors":"Tianlie Luo ,&nbsp;Jingjing Shi ,&nbsp;Ping Zhang ,&nbsp;Shuang Yang ,&nbsp;Guo Liu ,&nbsp;Willie J.G.M. Peijnenburg","doi":"10.1016/j.aquatox.2025.107330","DOIUrl":"10.1016/j.aquatox.2025.107330","url":null,"abstract":"<div><div>In this study, <em>Scenedesmus obliquus</em> (<em>S. obliquus</em>) has been employed as a model organism to investigate the bioaccumulation, metabolism, and toxicity mechanisms of tricresyl phosphate (TCP). The results indicated that <em>S. obliquus</em> enhanced TCP degradation in water by 97 % after 14 days. The bioaccumulation factor of tricresyl phosphate in <em>S. obliquus</em> were calculated to be 8. When exposed to a high concentration of TCP (160 μmol/L), the algal growth rate was initially negative at 24 h, but gradually recovered over time. By 96 h, the inhibition rate was 64.74 % and the EC₅₀ values was determined to be 86.41 μmol/L. Prolonged exposure to TCP substantially inhibited photosynthesis in <em>S. obliquus</em>, as indicated by a significant reduction in chlorophyll content. The addition of humic acid (HA), a representative substance of dissolved organic matter, exacerbated TCP toxicity by increasing ROS production, indicating a synergistic effect between HA and TCP. Conversely, a mixed nitrogen source reduced TCP toxicity. Four TCP metabolites were identified, resulting from hydroxylation, ketonization, hydrolysis, and ester bond cleavage. ECOSAR analysis revealed that these metabolites exhibit lower toxicity compared to TCP. These findings indicate that metabolic transformations within the algae may mitigate TCP toxicity, whereas HA significantly exacerbates TCP-induced oxidative stress. This study offers novel insights into the ecological risks of TCP in aquatic environments, especially in the presence of natural organic matter.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107330"},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short and long-term exposure of marine medaka (Oryzias melastigma) to an aluminum salt and to the cocktail of elements released by galvanic anode cathodic protection (GACP): impact on survival, growth, reproduction and behavior","authors":"Mélanie Blanc-Legendre ,&nbsp;Thomas Gosselin ,&nbsp;Christelle Caplat ,&nbsp;Georges Safi ,&nbsp;Xavier Cousin","doi":"10.1016/j.aquatox.2025.107329","DOIUrl":"10.1016/j.aquatox.2025.107329","url":null,"abstract":"<div><div>Galvanic Anodes as a Cathodic Protection system (GACP) are massively used to prevent corrosion of metallic materials that are immersed in seawater. The dissolution of these alloys leads to the release of metallic elements, in particular the principal constituent aluminum (Al), that is predicted to increase along with the exponential development of offshore wind farms. A limited number of studies have examined Al toxicity to marine fish, alone or in cocktail. To our knowledge, effects of GACP-released elements on fish remain completely unexplored. The present work evaluated acute and chronic toxicity of aluminum salt and of the cocktail of elements released by a functioning GACP system using marine medaka (<em>Oryzias melastigma</em>) as a model species. Embryo-larval development was monitored in acute exposure settings (phenotypic observations, locomotion) and various traits were analyzed in adults exposed for 150 days to the chemicals: growth, reproductive effort, and 4 behavioral traits (shoaling behavior, spontaneous activity, anxiety, exploratory abilities). Embryonic development of the F1 generation was monitored. Results show no significant adverse effect in developmental or in adult stages with regards to survival, growth, and reproductive output. Nonetheless, significant increase in anxiety level in the novel tank diving test was reported after exposure to Al at high dose (191 µg L^-1 and 333 µg L^-1 for respectively dissolved and total Al concentration). The same trend was observed in the GACP condition together with a tendency to form tighter shoals (291 µg L^-1 and 394 µg L^-1 for respectively dissolved and total Al concentration). Overall, the present data support limited toxicity from GACP systems to a model marine fish species in environmental conditions.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"283 ","pages":"Article 107329"},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low lipid levels caused by bisphenol S exposure trigger neuroinflammation and apoptosis in the brain of zebrafish","authors":"Zehui Du ,&nbsp;Shuai Li ,&nbsp;Hongyuan Peng ,&nbsp;Jiali Li ,&nbsp;Ze Li ,&nbsp;Shaoguo Ru ,&nbsp;Weiwei Wang","doi":"10.1016/j.aquatox.2025.107328","DOIUrl":"10.1016/j.aquatox.2025.107328","url":null,"abstract":"<div><div>Bisphenol S (BPS), as an environmental pollutant, is known to reduce brain lipid levels and induce neurotoxicity. However, whether brain lipid imbalance can induce neurotoxicity has not yet been clarified. Here, wild-type zebrafish and <em>apoEb</em> mutant zebrafish were used to investigate the effect of BPS on the macrophages proliferation and microglia mobilization caused by the decrease of cerebral lipids and its potential neurotoxic effects. The zebrafish exposed to BPS (1, 10, or 100 μg/L) from 2 hours after fertilization (hpf) to 3 days after fertilization (dpf) displayed microglial aggregation, as well as a decrease in brain lipid content. Lipidomic analyses of the brains and plasma of 50 dpf zebrafish exposed to BPS were used to identify key lipids, including lysophosphatidylcholine and phosphatidylcholine in brain and phosphatidylcholine in plasma. The <em>apoEb</em> mutant zebrafish as a hyperlipidemia model was used to further demonstrate that BPS-induced lipid reduction increased the number of microglia in the brain. Our data provide new insight into the mechanism by which pollutants cause neurotoxicity.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107328"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress and mitochondrial dysfunctions induced by cyanobacterial microcystin-LR in primary grass carp hepatocytes","authors":"Miao He ,&nbsp;Hui Wang ,&nbsp;Jianping Fu ,&nbsp;Jiming Ruan ,&nbsp;Fugui Li ,&nbsp;Ximei Liang ,&nbsp;Lili Wei","doi":"10.1016/j.aquatox.2025.107327","DOIUrl":"10.1016/j.aquatox.2025.107327","url":null,"abstract":"<div><div>Microcystin-LR (MC-LR), a cyclic heptapeptide produced by freshwater cyanobacteria, induces a range of liver injuries. However, the mechanisms underlying MC-LR toxicity in primary hepatocytes of aquatic organisms remains poorly understood. In this study, we investigated the effects of MC-LR on oxidative stress and mitochondrial function using primarily cultured grass carp hepatocytes. The results revealed that IC₅₀ of MC-LR on grass carp primary liver cells for 24 hours was 2.40 μmol/L. Based on 24h-IC₅₀, concentrations of 0, 0.30, 0.60, and 1.20 μmol/L were used in subsequent experiments. MC-LR exposure led to a significant reduction in cell viability, induced abnormal cell morphology, and caused plasma membrane rupture, as indicated by elevated LDH activity in a concentration-dependent manner. Additionally, MC-LR exposure induced oxidative stress, resulting in increased ROS levels and downregulation of genes associated with oxidative stress, including <em>keap1, nrf2, cat, sod1, gpx, gst</em>, and <em>gr</em> (<em>P</em>&lt;0.05). Furthermore, the electron microscopy results showed that MC-LR caused damage to the ultrastructure of primary hepatocytes, including mitochondrial membrane rupture, vacuolation, and induction of mitochondrial autophagy. Moreover, MC-LR exposure elevated intracellular Ca²⁺ concentration, reduced MMP and ATP levels, and inhibited mitochondrial respiratory chain complex I activity (<em>P</em>&lt;0.05). qRT-PCR analysis demonstrated that MC-LR treatment significantly decreased the transcriptional levels of genes related to mitochondrial quality control including <em>pgc-1α, tfam, nrf1, drp1, opa1, mfn1</em>, and <em>mfn2</em> (<em>P</em>&lt;0.05). Collectively, our findings highlight that MC-LR causes oxidative stress and impairs mitochondrial function, leading to further hepatocyte damage, which provides insights into the mechanisms of MC-LR-induced hepatotoxicity and offers valuable references for further investigations.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107327"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the immunotoxic effects of benzo[a]pyrene on Mytilus coruscus through histopathological, enzymatic, and transcriptomic analyses","authors":"Xiaoya Wang ,&nbsp;Yaru Li ,&nbsp;Kaida Xu ,&nbsp;Qingyang Li ,&nbsp;Shuang Yan ,&nbsp;Yingying Ye ,&nbsp;Pengzhi Qi ,&nbsp;Hongfei Li","doi":"10.1016/j.aquatox.2025.107326","DOIUrl":"10.1016/j.aquatox.2025.107326","url":null,"abstract":"<div><div>Benzo[a]pyrene (BaP) is a representative polycyclic aromatic hydrocarbon (PAH) known for its significant toxicity and environmental persistence, capable of causing mutations, deformities, and cancer in aquatic organisms. However, systematic studies on the effects of BaP exposure on histological damage, cell apoptosis, enzyme activity changes, and gene expression in <em>Mytilus coruscus</em> (M<em>. coruscus</em>), an important ecological indicator species, remain scarce. In this study, the biological effects of BaP on M<em>. coruscus</em> and the immunotoxic mechanisms following BaP exposure were evaluated using histological analysis, TUNEL assay, enzyme activity assays, and transcriptome sequencing. Our findings revealed notable histopathological changes due to BaP exposure, including hemocyte infiltration, atrophy, and deformation of digestive tubules in the digestive glands, as well as epithelial cell detachment and deformation in gills. Antioxidant enzyme activities (CAT, GSH-Px, SOD, T-AOC) varied significantly across tissues under BaP stress. Additionally, significant DNA fragmentation and increased apoptosis were observed in BaP-exposed groups compared to controls. Transcriptome analysis showed that after BaP exposure, nucleotide excision repair and innate immune response pathways were suppressed, while the metabolism of xenobiotics by cytochrome P450, glutathione biosynthesis, and apoptosis pathways were upregulated. These results elucidate the toxic mechanisms of BaP on M<em>. coruscus</em> and the immunotoxic responses of the mussels. This study enhances our understanding of how BaP and similar pollutants affect marine bivalves, providing valuable insights for environmental monitoring and pollutant management strategies.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107326"},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pups on mercury: Tracking early life exposure on South American fur seals (Arctocephalus australis) and South American sea lions (Otaria byronia) in the Southeastern Pacific","authors":"I. Peña-Galindo ,&nbsp;B. Bernales-Santolaya ,&nbsp;F. Montalva ,&nbsp;J. Gutiérrez ,&nbsp;F. Quesada-Alvarado ,&nbsp;S. Navarrete-Quintanilla ,&nbsp;V. Robbins ,&nbsp;D.J. Perez-Venegas ,&nbsp;G. Cortés-Hinojosa ,&nbsp;M. Seguel ,&nbsp;G. Chiang","doi":"10.1016/j.aquatox.2025.107325","DOIUrl":"10.1016/j.aquatox.2025.107325","url":null,"abstract":"<div><div>Mercury exists in three forms: elemental, inorganic, and organic, with methylmercury (MeHg) being the most concerning due to its ability to cross cellular barriers and bioaccumulate, particularly in marine mammals, where over 90 % of total mercury is in the MeHg form. Despite its importance, there is limited data on mercury bioaccumulation in marine mammals and maternal transfer mechanisms in the Southern Hemisphere. Pinnipeds, as ocean sentinels, are valuable for monitoring contaminants due to their ecological and biological traits. This study investigates mercury burdens and maternal transfer of mercury in South American sea lions (SASL) and South American fur seals (SAFS). Samples of clots and vibrissae from SASL pups and clots and milk from SAFS pups and females were analyzed. Total mercury (THg) levels in SASL ranged from 8.36 to 305.43 μg/Kg w.w. in clots and from 3071.8 to 28,034.5 μg/Kg d.w. in vibrissae. In SAFS, THg levels in clots ranged from 0.40 to 358.77 μg/Kg w.w. and in milk from 3.4 to 14.1 μg/Kg w.w. Significant differences were observed between newborn pups of both species, with a positive correlation between THg levels in clots from SAFS pups and females and between clots and vibrissae in SASL pups, indicating maternal transfer during gestation. Additionally, THg levels in SAFS pups decreased over time, suggesting biodilution. These species allow us to have a long term monitoring in both colonies and two different areas in Chile and are relevant findings to food security and the treatment of heavy metal contamination.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107325"},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicokinetic and liver proteomic study of the Chinese rare minnow (Gobiocypris rarus) exposed to F-53B","authors":"Hongbo Yang ,&nbsp;Chan Xu ,&nbsp;Jieyu Song ,&nbsp;Jin Li ,&nbsp;Chan Zhang ,&nbsp;Chunli Teng ,&nbsp;Kai Ma ,&nbsp;Feng Xie","doi":"10.1016/j.aquatox.2025.107312","DOIUrl":"10.1016/j.aquatox.2025.107312","url":null,"abstract":"<div><div>Perfluorooctane sulfonic acid (PFOS) and its alternative 6:2 chlorinated polyfluoroethersulfonate (6:2 Cl-PFESA, also known as F-53B), are frequently detected in a variety of environmental and human samples. These substances have been associated with hepatotoxic effects, including disorders in lipid metabolism and oxidative stress. However, the molecular mechanisms underlying the causal relationship between exposure to F-53B and hepatotoxicity remain inadequately understood. This study investigated the toxicokinetics and mechanisms of hepatotoxicity associated with prolonged exposure to F-53B in adult Chinese rare minnows. Specifically, 5-month-old adult Chinese rare minnow was exposed to concentrations of 10 μg/L and 200 μg/L of F-53B for a duration of 28 days for bioaccumulation assessment, followed by a 14-day period for metabolic evaluation. The findings indicated that the bioaccumulation of F-53B in the tissues was positively correlated with the exposure concentrations. The logarithmic bioconcentration factor (Log BCF28d) was determined to be 2.67 ± 0.02 for the low concentration group and 2.27 ± 0.01 for the high concentration group. The calculated half-lives (t_1/2) were 18.50 ± 1.67 days and 21.38 ± 0.31 days for the respective concentration groups. F-53B protein exhibited a distinct tissue-specific distribution in adult Chinese rare minnow, with the following order of enrichment: Blood &gt; Liver &gt; Gonad &gt; Gill &gt; Intestine &gt; Brain &gt; Muscle. F-53B was primarily concentrated in the blood and liver, where the protein content was significantly higher. Exposure to F-53B for 28 days significantly elevated biochemical levels associated with lipid metabolism and increased the activities of the enzymes FAS, PPARα, and ACC in the liver. This exposure also resulted in impairment of the hepatic oxidative system in the Chinese rare minnow, with F-53B significantly reducing most of the measured markers related to oxidative stress (e.g., GSH, SOD, CAT, and MDA). Proteomic analysis indicated that the toxicity of F-53B regulates the expression of proteins across several functional classes. Based on the functional information of the differential proteins provided in UniProt and KEGG, they were categorized into five main categories: Cellular Processes, Environmental Information Processing, Genetic Information Processing, Metabolism, and Organismal Systems. This study indicate that F-53B is bioaccumulative and persistent in Chinese rare minnow, and can further induce oxidative stress and lipid metabolism disorders. Combined with proteomic research methods, the toxicological effects of F-53B on Chinese rare minnow can be better explained.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107312"},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics study reveals DON-induced intestinal toxicity in adult zebrafish through disruption of amino acid metabolism and sphingolipid signaling pathway","authors":"Yuxiang Wang ,&nbsp;Luhan Wang ,&nbsp;Yaowen Du ,&nbsp;Feng Yao ,&nbsp;Miaomiao Zhao ,&nbsp;Chenggang Cai ,&nbsp;Ruiyu Zhu ,&nbsp;Suqin Shao","doi":"10.1016/j.aquatox.2025.107324","DOIUrl":"10.1016/j.aquatox.2025.107324","url":null,"abstract":"<div><div>Deoxynivalenol (DON), a prevalent mycotoxin contaminating cereal crops globally, poses significant threats to animal and human health through its gastrointestinal toxicity. While DON-induced intestinal damage has been documented in mammals, its metabolic mechanisms in aquatic species remain poorly understood, particularly in adult zebrafish models that offer unique advantages for toxicological studies. Multi-omics analysis revealed 16 key differential metabolites (9 upregulated, 7 downregulated) associated with amino acid metabolism and carbohydrate homeostasis. Pathway enrichment analysis identified significant perturbations in 2-oxocarboxylic acid metabolism and sphingolipid signaling, suggesting mitochondrial dysfunction and epithelial barrier disruption as primary toxicity mechanisms. This study establishes the first adult zebrafish model for DON intestinal toxicity evaluation, demonstrating its utility in revealing conserved metabolic targets across species. The identified pathway-specific biomarkers provide novel insights for developing dietary interventions against mycotoxin exposure.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"282 ","pages":"Article 107324"},"PeriodicalIF":4.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}