Aquatic Toxicology最新文献

{"title":"Safe concentration, unsafe effects: Impact of BPA on antioxidant function in the hepatopancreas and ovarian gene expression in oriental river prawns (Macrobrachium nipponense)","authors":"Weihong Zhao,&nbsp;Xirui Zheng,&nbsp;Fengjuan Jiang,&nbsp;Jintao Liu,&nbsp;Shuhao Wang,&nbsp;Jiangtao Ou","doi":"10.1016/j.aquatox.2024.107103","DOIUrl":"10.1016/j.aquatox.2024.107103","url":null,"abstract":"<div><div>This study investigated the effects of Bisphenol A (BPA), a common endocrine-disrupting chemical, on the antioxidant enzyme activities in the hepatopancreas and the expression of genes related to ovarian development in oriental river prawns (<em>Macrobrachium nipponense</em>). The 24hLC50 and 48hLC50 values for BPA were 80.59 mg/L and 63.90 mg/L, respectively, with a safe concentration of 12.06 mg/L. Prawns were exposed to low (4.85 mg/L), safe (12.06 mg/L), and high (30.00 mg/L) concentrations of BPA for 10 days to measure enzyme activities, and for 20 days followed by 7 days in BPA-free water to measure gene expression.</div><div>Short-term exposure (12 h, 1d, 3d) to low concentration BPA did not significantly affect superoxide dismutase (SOD) activity in the hepatopancreas (<em>P</em> &gt; 0.05), but long-term exposure (6d, 10d) significantly reduced SOD activity (<em>P</em> &lt; 0.05). Catalase (CAT) activity showed no significant changes throughout the low concentration exposure period (<em>P</em> &gt; 0.05). At safe and high concentrations, SOD and CAT activities significantly decreased after 12 h of exposure (<em>P</em> &lt; 0.05).</div><div>BPA affected heat shock protein 90 (<em>HSP90</em>) expression in the ovary, with low concentration BPA significantly upregulating <em>HSP90</em> after 1 day (<em>P</em> &lt; 0.05), but returning to normal levels after 10 and 20 days. At the safe concentration, HSP90 was significantly upregulated at all three sampling points (1d, 10d, 20d) (<em>P</em> &lt; 0.05), while high concentration exposure led to significant upregulation only on day 10 (<em>P</em> &lt; 0.05).</div><div>Low concentration BPA had no significant effect on Cathepsin B (<em>CB</em>) and Cathepsin L (<em>CL</em>) gene expression in the ovaries (<em>P</em> &gt; 0.05). However, safe concentration exposure promoted <em>CB</em> expression on days 1, 10, and 20 (<em>P</em> &lt; 0.05), while high concentration exposure significantly increased <em>CB</em> expression on day 1 (<em>P</em> &lt; 0.05), with levels returning to normal on days 10 and 20. <em>CL</em> expression significantly increased after 20 days of exposure to both safe and high concentrations (<em>P</em> &lt; 0.05). Gene expression levels in the ovaries returned to normal after transfer to BPA-free water, with <em>HSP90</em> and <em>CB</em> normalizing by day 1, and <em>CL</em> by day 7.</div><div>These results indicate that even safe concentrations of BPA impose stress on the hepatopancreas and increase the expression of <em>HSP90, CB</em>, and <em>CL</em> genes in the ovaries, affecting ovarian development. And, these effects are reversible within a certain period after the removal of BPA.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107103"},"PeriodicalIF":4.1,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The iodinated contrast agent diatrizoic acid has an impact on the metabolome of the mollusc Dreissena polymorpha","authors":"Laura Fuster ,&nbsp;Christelle Bonnefoy ,&nbsp;Aurélie Fildier ,&nbsp;Alain Geffard ,&nbsp;Carine Arnaudguilhem ,&nbsp;Sandra Mounicou ,&nbsp;Odile Dedourge-Geffard ,&nbsp;Gaëlle Daniele ,&nbsp;Emmanuelle Vulliet","doi":"10.1016/j.aquatox.2024.107087","DOIUrl":"10.1016/j.aquatox.2024.107087","url":null,"abstract":"<div><p>The occurrence of iodinated contrast agents (ICAs) in the aquatic environment is relatively well documented, showing that these compounds can be found at several µg/L in natural waters, and up to hundreds of µg/L in waste water treatment plants inlets. Nevertheless, only few studies address their potential impacts and fate in aquatic organisms mainly because these compounds are considered non-toxic due to their intrinsic properties. However, as aquatic organisms are continuously exposed to these compounds, they could nonetheless induce some adverse effects on aquatic populations like filter feeder organisms. To verify this, we exposed model organisms, <em>Dreissena polymorpha</em> mollusks, to 100 µg/L of an ICA, diatrizoic acid (DTZ), to determine the potential biological effects caused by this compound using a non-targeted metabolomic approach based on liquid chromatography coupled to high resolution mass spectrometry. Metabolic profiles showed a slight effect of DTZ, with some metabolome variations linked to exposure. Indeed, to avoid any misinterpretation of DTZ effects, we also studied the natural evolution of the metabolome over time in unexposed mussels, showing that control mussels exhibited metabolomic changes over the exposure period. During DTZ exposure, we showed that the carnitine shuttle pathway of fatty acids and pyrimidine metabolisms were impacted, leading to dysregulation of mussels’ energy metabolism. Thus, this study demonstrates for the first time that compounds considered non-toxic like ICAs can have an impact on aquatic organisms such as bivalves by slightly modulating their metabolome.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107087"},"PeriodicalIF":4.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxic effects of ZnO NPs on immune response and tissue pathology in Mytilus galloprovincialis","authors":"Zihan Xing ,&nbsp;Zimin Cai ,&nbsp;Liuya Mi ,&nbsp;Juan Zhang ,&nbsp;Jiaying Wang ,&nbsp;Lizhu Chen ,&nbsp;Mingzhe Xu ,&nbsp;Bangguo Ma ,&nbsp;Ruijia Tao ,&nbsp;Bowen Yang ,&nbsp;Xinmeng Lv ,&nbsp;Lei Wang ,&nbsp;Yancui Zhao ,&nbsp;Xiaoli Liu ,&nbsp;Liping You","doi":"10.1016/j.aquatox.2024.107102","DOIUrl":"10.1016/j.aquatox.2024.107102","url":null,"abstract":"<div><p>Nano-zinc oxide (ZnO NPs), as widely used nanomaterials, are inevitably released into aquatic environments, posing potential threats to aquatic organisms. <em>Mytilus galloprovincialis</em> is a bivalve species sensitive to changes in marine ecological environments, but there has been limited research on its toxicity response to ZnO NPs. Therefore, we selected <em>M. galloprovincialis</em> as the research subject and exposed them to 50 µg/L ZnO NPs for 96 h and 30 days to determine the dissolution of ZnO NPs in seawater and their distribution in <em>M. galloprovincialis</em>. The toxicity of ZnO NPs in <em>M. galloprovincialis</em> was then evaluated through gene expression, tissue pathology, and cellular immune response. The results showed that ZnO NPs could enrich Zn in various tissues of the mussel, in the order of gills &gt; hepatopancreas &gt; adductor muscle &gt; mantle. Seven immune-related genes including four heat shock protein genes (<em>HSPA12A, sHSP24.1, sHSP22, TCTP</em>) and <em>three apoptotic genes</em> (<em>Ras, p63</em> and <em>Bcl-2</em>) were altered to varying degrees. There was a downward trend in lysosomal membrane stability of <em>M. galloprovincialis</em> after exposure to ZnO NPs for 96 h and 30 days, while ROS and apoptosis rates increased significantly. Furthermore, the seven genes, apoptosis, LMS, and ROS were dependent on exposure time, treatment, and their interaction. Histopathological damage included disorganisation of hepatopancreas epithelial cells, gill filament swelling, and contraction of blood sinuses. These results indicated that ZnO NPs exerted toxicity in <em>M. galloprovincialis</em>, affecting the immune system, resulting in changes in the expression of immune-related genes and ultimately leading to histopathological changes. Our research findings could contribute to systematically understand the impact of ZnO NPs on bivalves in aquatic environments and provide a theoretical basis for marine pollution assessment.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107102"},"PeriodicalIF":4.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity and mechanisms of perfluorobutane sulfonate (PFBS) in umbilical cord fibroblast cells of Yangtze finless porpoise","authors":"Maaz Ahmad ,&nbsp;Chenyan Hu ,&nbsp;Mengyuan Liu ,&nbsp;Haobo Zhang ,&nbsp;Syed Ata Ur Rahman Shah ,&nbsp;Ghulam Nabi ,&nbsp;Yujiang Hao ,&nbsp;Lianguo Chen","doi":"10.1016/j.aquatox.2024.107098","DOIUrl":"10.1016/j.aquatox.2024.107098","url":null,"abstract":"<div><p>Yangtze finless porpoises (YFP) accumulate high levels of per- and polyfluoroalkyl substances (PFASs). However, the health impacts of PFASs to YFP are still unknown because it is technically and ethically unfeasible to use the critically endangered YFP in toxicological exposures. To uncover the potential toxicities of PFASs to YFP, this study exposed a YFP umbilical cord fibroblast cell line to perfluorobutane sulfonate (PFBS), an emerging PFASs pollutant in the aquatic environments. After exposure, the cytotoxicity and mechanisms of PFBS were explored. Our preliminary experiments found that PFBS compromised the cell viability in a concentration and duration dependent manner. In an exposure of 48-h duration, the maximum no observed effect concentration (NOEC) of PFBS was determined to be 400 µM. High-throughput proteomics were then conducted to identify the differentially expressed proteins in YFP cells exposed to 400 µM PFBS for 48 h. The results found that PFBS exposure significantly perturbed the proteome fingerprints of YFP umbilical cord fibroblast cells. Functional annotation of differential proteins showed that PFBS had the potential to impair a variety of biological processes associated with the immunity, oxidative stress, metabolism, and proteolysis. Consistently, the intracellular levels of reactive oxygen species (ROS) and proinflammatory cytokine IL-1β were significantly increased by PFBS in YFP umbilical cord fibroblast cells. Overall, this study highlights the toxic effects of emerging PFASs on YFP and provides reference data to evaluate the health risks of aquatic pollution under the context of national YFP protection. To our knowledge, this is the first omics study using YFP umbilical cord fibroblast cells in ecotoxicology of PFASs, which is applicable to various cetacean species and pollutants.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107098"},"PeriodicalIF":4.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the hidden threats: Genotoxic effects of microplastics on freshwater fish","authors":"Maiara Menezes ,&nbsp;Franco Teixeira de Mello ,&nbsp;Lucia Ziegler ,&nbsp;Bruno Wanderley ,&nbsp;Juan Manuel Gutiérrez ,&nbsp;Juliana Deo Dias","doi":"10.1016/j.aquatox.2024.107089","DOIUrl":"10.1016/j.aquatox.2024.107089","url":null,"abstract":"<div><p>New evidence regarding the risks that microplastics (MP) ingestion pose to human and wildlife health are being revealed with progress made in ecotoxicological research. However, comprehensive and realistic approaches that evaluate multiple physiological responses simultaneously are still scarce despite their relevance to understand whole-organism effects. To address this information gap, we performed an experiment to assess the effects of MP on freshwater fish physiology from the molecular to the organismal level. Using a model species of global commercial importance (<em>Cyprinus carpio</em>) and MP type (recycling industry fragments), size (range between 125-1000 µm), and two concentrations of environmental relevance (0.75 and 8.25 µg/L). Experimental design included 5 blocks containing 3 treatment levels each one: control, low, and high MP concentration, with 6 fish each aquarium (5 blocks x 3 treatments x 6 fish per aquarium = 90 fish). Our results suggest that, under the experimental conditions applied, MP exposure did not cause adverse effects at the morphological (variation in size of gut), metabolic (variation of standard metabolic rate), or ecological (growth performance) levels. Nonetheless, we observed an increased frequency of micronucleated cells with increasing MP concentration (df = 42, t-value = 3.68, p-value &lt; 0.001), showing the potential genotoxicity of MP, which can clearly harm fish health in long-term. Thus, despite being a highly resistant species, exposure to MP may generate negative effects in juvenile <em>C. carpio</em> at cellular or subcellular levels. Our findings highlight that the manifestation of MP effects may vary over time, emphasizing the need for future studies to consider longer exposure durations in experimental designs.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107089"},"PeriodicalIF":4.1,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testing the sensitivity of the medaka Transgenic Eleuthero-embryonic THYroid-Specific assay (TETHYS) to different mechanisms of action","authors":"Elise Pesce ,&nbsp;Jessica Benitez-Gonzalez ,&nbsp;Andrew J. Tindall ,&nbsp;Gregory F. Lemkine ,&nbsp;Barbara Robin-Duchesne ,&nbsp;Laurent M. Sachs ,&nbsp;et David Du Pasquier","doi":"10.1016/j.aquatox.2024.107081","DOIUrl":"10.1016/j.aquatox.2024.107081","url":null,"abstract":"<div><div>There are many concerns about the impacts of Endocrine-Disrupting Chemicals on both wildlife and human populations. A plethora of chemicals have been shown to interfere with the Hypothalamic–Pituitary–Thyroid (HPT) axis in vertebrates. Disruption of the HPT axis is one of main endocrine criteria considered for the regulation of chemicals, along with the estrogen axis, androgen axis and steroidogenesis (EATS). In response to these concerns, the Organization for Economic Cooperation and Development (OECD) initiated the validation of test guidelines (TGs) covering the EATS modalities. Regarding thyroid activity and/or disruption assessment, three OECD TGs are validated, all of them using amphibians. To date, no OECD TGs based on fish are available for the detection of Thyroid Active Chemicals (TACs). To fill this gap, we developed a new test for the detection of TACs, the TETHYS assay (Transgenic Eleuthero-embryonic THYroid-Specific assay). This assay uses a medaka (<em>Oryzias latipes</em>) transgenic line Tg(<em>tg:eGFP</em>) expressing Green Fluorescent Protein in the thyroid follicles, under the control of the thyroglobulin promoter. This assay is performed at eleuthero-embryonic life-stages with an exposure length of 72 h. In the present study, the following reference chemicals with known thyroid hormone system mechanism of action have been tested: methimazole, sodium perchlorate, sodium tetrafluoroborate, diclofenac, iopanoic acid, sobetirome, NH-3 and 1–850. Except for the thyroid receptor antagonists, all chemicals tested were identified as thyroid active, modifying the total fluorescence and the size of the thyroid follicles. To investigate the test specificity, we tested three chemicals presumed to be inert on the HPT axis: cefuroxime, abamectin and 17α-ethinylestradiol. All were found to be inactive in the TETHYS assay. This promising New Approach Methodology can serve as a foundation for the development of a new OECD TG in the frame of regulatory assessment of chemicals for thyroid activity.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107081"},"PeriodicalIF":4.1,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on low concentration deltamethrin toxicity mediated by phospholipase D in Chinese mitten crab (Eriocheir sinensis) ovary","authors":"Qi Sun ,&nbsp;Jun Dong ,&nbsp;Zehui Su ,&nbsp;Xuanyun Huang ,&nbsp;Xiaoning Gao ,&nbsp;Kun Hu ,&nbsp;Xiaoling Gong","doi":"10.1016/j.aquatox.2024.107090","DOIUrl":"10.1016/j.aquatox.2024.107090","url":null,"abstract":"<div><p>This study evaluates the impact of environmentally relevant, low-concentration deltamethrin exposure to <em>Eriocheir sinensis</em> ovaries. Our findings revealed that even at a concentration of 0.05 µg/L, deltamethrin exposure can induce significant ovarian toxicity through a 5-day exposure, with gradual amplification detected with time, demonstrating the toxicity amplification effect. Hematoxylin and Eosin staining revealed that low-concentration deltamethrin exposure produces pathological damage consistent with acute toxicity—yolk granules were dissolved and oocyte membranes were ruptured. High-throughput RNA-sequencing data indicated that the acute and low-concentration exposure groups involved completely different pathways and molecular functions, suggesting distinct mechanisms for their toxic effects. Following the identification of phospholipase D (PLD) as a potential core factor regulating the toxicity amplification effect of low concentration deltamethrin, we delved into subsequent mechanism studies using quantitative real-time PCR, immunofluorescence and enzyme-linked immunosorbent assay. Through the GnRH signaling pathway, increased PLD indirectly stimulates augmented estradiol secretion, subsequently inducing apoptosis by upregulating Cathepsin D, which can activate the key executioners of apoptosis—caspases (CASP3 and CASP7). In conclusion, low-concentration deltamethrin exposures can induce significant ovarian damage through apoptosis mediated by the upregulation of PLD in the ovaries of <em>Eriocheir sinensis</em> at environmentally relevant concentrations, which lays the preliminary theoretical groundwork for further elucidating the mechanism of toxicity amplification effect of pesticide exposure at low concentrations.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107090"},"PeriodicalIF":4.1,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing the neurotoxic effects of anatoxin-a and saxitoxin in zebrafish larvae","authors":"Irene Romero-Alfano ,&nbsp;Eva Prats ,&nbsp;Xavier Ortiz Almirall ,&nbsp;Demetrio Raldúa ,&nbsp;Cristian Gómez-Canela","doi":"10.1016/j.aquatox.2024.107088","DOIUrl":"10.1016/j.aquatox.2024.107088","url":null,"abstract":"<div><p>Global warming due to climate change, as well as freshwater eutrophication caused by anthropogenic activities are responsible, among other factors, for an increasing occurrence of harmful algal blooms (HABs) in aquatic systems. These can lead to the generation of cyanotoxins, secondary metabolites coming from cyanobacteria, producing adverse effects in living organisms including death. This research aims to study the effects that two neurotoxins, anatoxin-a (ATX-a) and saxitoxin (STX), have on living organisms. Once the stability of both compounds in water was determined for a 24 h period using ultra-high-performance liquid chromatography coupled to a triple quadrupole mass spectrometer (UPLC-MS/MS), zebrafish larvae were exposed to different levels of toxins (1 ng <em>L</em>⁻¹, 10 ng <em>L</em>⁻¹, 100 ng <em>L</em>⁻¹ and 1 μg <em>L</em>⁻¹) during 24 h. Behavioral studies including vibrational startle response (VSR), habituation to vibrational stimuli, basal locomotor activity (BLM) and visual motor response (VMR) were performed using Danio Vision system, and neurotransmitters (NTs) from 15-head pools of control and exposed zebrafish larvae were extracted and analyzed by UPLC-MS/MS. Both compounds induced hypolocomotion in the individuals, while 10 and 100 ng <em>L</em>⁻¹ of ATX-a significantly increased methionine (120 % and 126 %, respectively) and glutamate levels (118 % and 129 %, respectively). Saxitoxin enhanced 3-metoxytyramine (3-MT) levels at 1 ng <em>L</em>⁻¹ by 185 %. The findings of this study show that both studied cyanotoxins influence the behavior of zebrafish larvae as well as their metabolism.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107088"},"PeriodicalIF":4.1,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-alumina induced developmental and neurobehavioral toxicity in the early life stage of zebrafish, associated with mTOR","authors":"Ying Zhang ,&nbsp;Kaihong He ,&nbsp;Yanhong Wang ,&nbsp;Xinyue Guo ,&nbsp;Jin Chen ,&nbsp;Nan Shang ,&nbsp;Jianping Chen ,&nbsp;Ping Zhang ,&nbsp;Ling Zhang ,&nbsp;Qiao Niu ,&nbsp;Qinli Zhang","doi":"10.1016/j.aquatox.2024.107086","DOIUrl":"10.1016/j.aquatox.2024.107086","url":null,"abstract":"<div><p>The study aims to investigate the effects of nano-alumina (AlNPs) on the early development and neurobehavior of zebrafish and the role of mTOR in this process. After embryos and grown-up larvae exposed to AlNPs from 0 to 200 μg/mL, we examined the development, neurobehavior, AlNPs content, and mTOR pathway genes. Moreover, embryos were randomly administered with control, negative control, mTOR knockdown, AlNPs, and mTOR knockdown + AlNPs, then examined for development, neurobehavior, oxidative stress, neurotransmitters, and development genes. As AlNPs increased, swimming speed and distance initially increased and then decreased; thigmotaxis and panic-avoidance reflex substantially decreased in the high-dose AlNPs group; aluminum and nanoparticles considerably accumulated in the 100 μg/mL AlNPs group; AlNPs at high dose decreased mTOR gene and protein levels, stimulating autophagy via increasing ULK1 and ULK2. mTOR knockdown exacerbated the harm to normal development rate, eye and body length, and neurobehavior induced by AlNPs through raising ROS, SOD, and ACH levels but decreasing AchE activity and development genes. Therefore, AlNPs suppress neurobehavior through downregulating mTOR, and mTOR knockdown further aggravates their early development and neurobehavior loss, suggesting mTOR could be a potential target for the toxicity of AlNPs.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107086"},"PeriodicalIF":4.1,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multibiomarker approach to assess the effects of a BPA analogue-contaminated diet in the crab Carcinus aestuarii","authors":"Jacopo Fabrello ,&nbsp;Elisabetta Tarussio ,&nbsp;Alessia Romanello ,&nbsp;Anna Schiavon ,&nbsp;Vittorio Rubens Damoli ,&nbsp;Francesco Luisi ,&nbsp;Marco Roverso ,&nbsp;Sara Bogialli ,&nbsp;Valerio Matozzo","doi":"10.1016/j.aquatox.2024.107084","DOIUrl":"10.1016/j.aquatox.2024.107084","url":null,"abstract":"<div><p>Bisphenol A analogues are largely used plasticisers that are going to replace bisphenol A in many sectors. Due to this replacement, their discharge and presence in the marine coastal areas are increasing, with unknown consequences for organisms and the trophic chain. This study assessed the effects of three different bisphenols (BPAF, BPF and BPS) - alone or as a mixture - provided via food (exposed clams) to the crab <em>Carcinus aestuarii</em>. First, clams were exposed for two weeks to 300 ng/L of each of the three bisphenols and their mixture (100 ng/L of each) to allow the bioaccumulation of the contaminants in bivalves. Then, crabs were fed for two weeks with BPA analogue-exposed clams, while unexposed clams were used to feed control crabs. After 7 and 14 days, haemolymph, gills and hepatopancreas were collected from crabs to measure a battery of biomarkers indicative of cytotoxicity, oxidative stress and damage, neurotoxicity, physiological performance (respiration and excretion rate) and electron transport system activity. Lastly, bioaccumulation of BPA analogues was assessed by UHPLC-HRMS in crabs. Our findings revealed that BPA analogue-exposed clams were able to alter total haemocyte count, haemocyte size and their proliferation. The activity of immune enzymes, such as phosphatases and phenoloxidase was altered. Moreover, we observed an impairment of antioxidant and detoxifying enzymes like SOD, CAT, GST and GPX activities. Alterations of metabolism-involved enzymes and physiological parameters and increased oxidative damage to macromolecules like proteins, lipids, and DNA were also observed in crabs. Among BPA analogues, only bioaccumulation of BPAF, which has the highest Log_kow value among the tested bisphenols, was evidenced in crabs. Overall, the obtained results indicated that crabs, under the tested experimental conditions at least, underwent alterations in cellular, biochemical and physiological responses following a diet of bisphenol-exposed clams, suggesting a potential ecotoxicological risk in the marine food chain.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"276 ","pages":"Article 107084"},"PeriodicalIF":4.1,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0166445X24002546/pdfft?md5=69800ebb706760712f0aa80b6e89b367&pid=1-s2.0-S0166445X24002546-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142171770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}