Aquatic Toxicology最新文献

{"title":"Evidence for low bioavailability of dietary nanoparticulate cerium in a freshwater food chain","authors":"Lisa A. Golding ,&nbsp;Paul Callaghan ,&nbsp;Brad M. Angel ,&nbsp;Graeme E. Batley ,&nbsp;Grant Griffiths ,&nbsp;An Nguyen ,&nbsp;Tom Cresswell","doi":"10.1016/j.aquatox.2024.107228","DOIUrl":"10.1016/j.aquatox.2024.107228","url":null,"abstract":"<div><div>Radioactive ¹⁴¹Ce in ionic (I-Ce), nano (N-Ce, 11 ± 9 nm mean primary particle size ± standard deviation) and micron-sized (M-Ce, 530 ± 440 µm) forms associated with natural and artificial diets in natural river water and synthetic freshwater were used to measure the real-time biokinetics of dietary ¹⁴¹Ce assimilation in a freshwater food chain. The model food chain consisted of microalgae (<em>Raphidocelis subcapitata</em>), snails (<em>Potamopyrgus antipodarum</em>) and prawns (<em>Macrobrachium australiense</em>). Pulse-chase experiments showed that 91–100 % of all forms of cerium associated with all diets and water types were eliminated from the digestive system of the snail and prawn within 24 h, with no detectable cerium assimilation. The prawn and snail median elimination times (ET50) and elimination rates (Ke) for all cerium forms ranged from 0.05 to 1.7 d, and 30 to &gt;100 % per d, respectively. The pulse-chase results were supported by the autoradiographic evidence for N-Ce and M-Ce that confirmed no detectable assimilation and translocation within the tissue of the prawn over time. In contrast, the more soluble I-Ce was found to be associated in low quantities with the hepatopancreas in the prawn confirming that the lack of dissolution by N-Ce and M-Ce in the digestive environment of these organisms makes these forms less bioavailable. In addition, hetero-agglomeration of N-Ce and M-Ce resulted in particles that did not dissociate in digestive fluids and were too large to be assimilated thereby making them non-bioavailable. Based on the results from this study and from the literature review, the risk of N-Ce biomagnification and chronic dietary toxicity in freshwater ecosystems is no greater than the risk associated with M-Ce or I-Ce.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107228"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gender effects of nanoplastics and emerging contaminants mixtures in Mytilus galloprovincialis","authors":"J.M. Gonçalves ,&nbsp;M. Benedetti ,&nbsp;G. d'Errico ,&nbsp;F. Regoli ,&nbsp;M.J. Bebianno","doi":"10.1016/j.aquatox.2024.107219","DOIUrl":"10.1016/j.aquatox.2024.107219","url":null,"abstract":"<div><div>The reproduction of mussels occurs within the water column, and if gametogenesis is successful, gametes are exposed to the surrounding contaminants. Nanoplastics and other emerging contaminants have been gaining vast attention; however, their effects on the reproductive tissues of mussels with sex differentiation are scarce. Here, the effects of polystyrene nanoparticles (50 nm; 10 µg/L), the cytotoxic drug 5-fluorouracil (10 ng/L), and a mixture of the two were evaluated in the gonads of <em>Mytilus galloprovincialis</em> after a 21-day exposure for a multi-biomarker assessment, and after 28 days for the accumulation of nanoplastics. The effects on the activity of superoxide dismutase, catalase, glutathione-<em>S</em>-transferase, and lipid peroxidation were evaluated. Moreover, synergistic and antagonistic interactions in the mixture were calculated. A weight of evidence model was also used to elaborate on the hazardous level of biomarker results relative to polystyrene nanoparticles alone and in the mixture. The accumulation of nanoplastics appeared gender and time-specific, with females mostly compromised. According to the data set, a synergistic interaction between the cytotoxic drug and the nanoplastics makes the combination far more dangerous than individual stressors. The Weight Of Evidence model also confirms that females are more compromised at chronic exposure times than males. This study shows that the uptake, fate, and impact of emerging contaminants of concern can be significantly influenced by sex.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107219"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing predictive models for assessing LC50 of organic contaminants in Gammarus species using interpretable structural parameters","authors":"Mehran Karimi ,&nbsp;Eskandar Kolvari ,&nbsp;Mohammad Hossein Keshavarz ,&nbsp;Nadiya Koukabi","doi":"10.1016/j.aquatox.2025.107235","DOIUrl":"10.1016/j.aquatox.2025.107235","url":null,"abstract":"<div><div><em>Gammarus</em> species are crucial indicators of environmental health, making them important for ecological studies and water quality assessments. They offer a wide range of specific responses regarding the median lethal concentration (<em>LC₅₀</em>) of organic compounds. This research presents four predictive models to determine the <em>LC₅₀</em> of chemicals impacting selected gammarid amphipods: <em>Gammarus lacustris, Gammarus fasciatus, Gammarus pulex</em>, and <em>Gammarus pseudolimnaeus</em>. These species are recognized for their sensitivity to various pollutants and are among the most sensitive aquatic invertebrates. The new models provide straightforward methods for estimating the <em>pLC₅₀</em> (−log <em>LC₅₀</em>/molecular weight) of various organic compounds based on interpretable structural parameters including the number of effective functional groups, the types of atoms present, and various structural characteristics of organic molecules. This study aims to leverage the largest available experimental dataset compared to prior quantitative structure–activity relationship (QSAR) models for these gammarid amphipods. The dataset contained toxicity data for 91 compounds affecting <em>Gammarus fasciatus</em>, 50 for <em>Gammarus lacustris</em>, and 48 each for <em>Gammarus pseudolimnaeus</em> and <em>Gammarus pulex</em>, aligning with comparative QSAR models. External datasets included 13 compounds for <em>Gammarus fasciatus</em>, 2 for <em>Gammarus lacustris</em>, and 6 for <em>Gammarus pseudolimnaeus</em>. Efforts focus on using interpretable structural parameters of organic compounds rather than computer-based descriptors, as outlined in the existing QSAR models. For the species G<em>. fasciatus</em>, G<em>. lacustris</em>, G<em>. pseudolimnaeus</em>, and G<em>. pulex</em>, the R² ratios for the new models versus the best QSAR models are 0.915/0.728, 0.955/0.747, 0.976/0.769, and 0.970/0.768, respectively. The higher R² values in the new models demonstrate greater reliability and robustness in capturing the data's underlying relationships.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107235"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tributyl phosphate inhibits neurogenesis and motor functions during embryonic development in zebrafish","authors":"Gourav Chakraborty ,&nbsp;Bhagyashri Joshi ,&nbsp;Kedar Ahire ,&nbsp;Chinmoy Patra","doi":"10.1016/j.aquatox.2024.107203","DOIUrl":"10.1016/j.aquatox.2024.107203","url":null,"abstract":"<div><div>Tributyl phosphate (TBP), an organophosphate ester (OPE), is heavily used as a solvent in chemical industries, a plasticizer, and to extract radioactive molecules. Thus, widespread uses of TBP in industrialized countries led to the release of TBP and its metabolites, dibutyl phosphate (DBP) and monobutyl phosphate (MBP), in the environment and were detected in human samples. Accumulating these OPEs over time in humans and aquatic animals may develop toxicological effects. The reports also say TBP passes through the mother-fetal transmission route and may affect embryonic development. However, the impact of TBP and its metabolites on vertebrate development has been poorly studied. <em>Ex-utero</em> development, high fecundity, and optical transparency make the zebrafish a preferred model for toxicological evaluation. Thus, we aim to explore the toxic effects of TBP and its metabolites on aquatic animals using zebrafish as a model organism. Embryos in the chorion were incubated in 10–60 µM test chemicals from 6 to 48 h post fertilization (hpf), and analyzed the adverse effects on embryos. Our study found that 10–20 µM TBP inhibits neural growth, resulting in decreased spontaneous movement frequency and locomotive behavior without altering the overall embryonic growth and muscle functions. In contrast, DBP-treated embryos showed increased spontaneous movement frequency without changing the motor neuron growth and locomotive behavior. Further, in higher concentrations, TBP is teratogenic, and DBP is lethal to the embryos. Altogether, we found that TBP inhibits neurogenesis and motor behavior; however, its metabolite DBP is neuroexcitatory in zebrafish embryos.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107203"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential effects of corn cob biochar on mitigating pendimethalin-induced toxicity in Nile tilapia (Oreochromis niloticus): Effects on hematological, biochemical, antioxidant-immune parameters, and histopathological alterations","authors":"Rasha M. Reda ,&nbsp;Eman M. Zaki ,&nbsp;Ahmed A.A. Aioub ,&nbsp;Mohamed M.M. Metwally ,&nbsp;Fatma Mahsoub","doi":"10.1016/j.aquatox.2024.107213","DOIUrl":"10.1016/j.aquatox.2024.107213","url":null,"abstract":"<div><div>This study aims to examine the restorative impact of corn cob biochar (CCB) on pendimethalin (PMD)-induced toxicity in <em>Oreochromis niloticus</em>. Fish were divided into four groups: the first control group without treatment, the second group (CCB) exposed to 10 g CCB/L, the third group (PDM) exposed to 0.355 mg PDM/L, and the fourth group (PDM+ CCB) receiving both 0.355 mg PDM/L and 10 g CCB/L for 30 days. PDM exposure resulted in behavioral alterations, low survival rate (73.33 %), hematological and biochemical impairments, increased oxidative stress, suppressed immunity, and histopathological damage in gill, liver, and brain tissues. Co-treatment with CCB significantly alleviated these effects, as evidenced by improved survival rate (88.88 %), hematological, biochemical, and antioxidant-immune parameters and reduced histopathological alterations. In conclusion, CCB demonstrated a promising potential to mitigate PDM-induced toxicity in <em>O. niloticus</em> by enhancing physiological, biochemical, and histological resilience.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107213"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent consensus-based predictions of early life stage toxicity in fish tested in compliance with OECD Test Guideline 210","authors":"Souvik Pore ,&nbsp;Alexia Pelloux ,&nbsp;Anders Bergqvist ,&nbsp;Mainak Chatterjee ,&nbsp;Kunal Roy","doi":"10.1016/j.aquatox.2024.107216","DOIUrl":"10.1016/j.aquatox.2024.107216","url":null,"abstract":"<div><div>Early life stage (ELS) toxicity testing in fish is a crucial test procedure used to evaluate the long-term effects of a wide range of chemicals, including pesticides, industrial chemicals, pharmaceuticals, and food additives. This test is particularly important for screening and prioritizing thousands of chemicals under the Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH) regulation. In silico methods can be used to estimate the toxicity of a chemical when no experimental data is available and to reduce the cost, time, and resources involved in the experimentation process. In the present study, we developed predictive Quantitative Structure-Activity Relationship (QSAR) models to assess chronic effects of chemicals on ELS in fish. Toxicity data for ELS in fish was collected from two different sources, i.e. J-CHECK and eChemPortal, which contain robust study summaries of experimental studies performed according to OECD Test Guideline 210. The collected data included two types of endpoints – the No Observed Effect Concentration (NOEC) and the Lowest Observed Effect Concentration (LOEC), which were utilized to develop the QSAR models. Six different partial least squares (PLS) models with various descriptor combinations were created for both endpoints. These models were then employed for intelligent consensus-based prediction to enhance predictability for unknown chemicals. Among these models, the consensus model – 3 (Q²_F1 = 0.71, Q²_F2 = 0.71) and individual model – 3 (Q²_F1 = 0.80, Q²_F2 = 0.79) exhibited most promising results for both the NOEC and LOEC endpoints. Furthermore, these models were validated experimentally using experimental data from nine different industrial chemicals provided by Global Product Compliance (Europe) AB. Lastly, the models were used to screen and prioritize chemicals obtained from the Pesticide Properties (PPDB) and DrugBank databases.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107216"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurodevelopmental toxicity and mechanism of action of monoethylhexyl phthalate (MEHP) in the developing zebrafish (Danio rerio)","authors":"Chaoqiong Wu ,&nbsp;Guanghua Mao ,&nbsp;Xiang Ji ,&nbsp;Yao Chen ,&nbsp;Xin Geng ,&nbsp;Emmanuel Sunday Okeke ,&nbsp;Yangyang Ding ,&nbsp;Liuqing Yang ,&nbsp;Xiangyang Wu ,&nbsp;Weiwei Feng","doi":"10.1016/j.aquatox.2024.107230","DOIUrl":"10.1016/j.aquatox.2024.107230","url":null,"abstract":"<div><div>Monoethylhexyl phthalate (MEHP) is the primary metabolite of di(2-ethylhexyl) phthalate (DEHP), the most prevalent phthalate plasticiser globally. It has been demonstrated that MEHP exerts more potent toxic effects than DEHP. Nevertheless, the full extent of the toxicity of MEHP to neurodevelopmental organisms remains unclear. Accordingly, the present study was designed to investigate the neurodevelopmental toxicity of MEHP exposure and the underlying molecular mechanisms. Zebrafish juveniles were exposed to different concentrations of MEHP (7.42, 14.84, 29.68 and 74.2 μg/L) for a period of four weeks. Immunohistological evidence indicated that MEHP exposure resulted in oxidative stress and apoptosis in the developing zebrafish brain. Subsequently, the neurobehaviour of zebrafish larvae was evaluated, and it was determined that MEHP significantly disrupted their locomotor capacity, motor vigor, and social conduct. Furthermore, HE staining revealed damage to brain neurons, which may be linked to impaired synthesis and conduction of inter-synaptic neurotransmitters. Transcriptomic analyses indicated that MEHP may affect the expression levels of genes in the P53 signalling pathway and signalling pathways related to the development of the nervous system. This results in impaired functions, including nerve conduction and neuronal development. Additionally, it induces oxidative stress, which leads to significant brain cell apoptosis and, ultimately, neurotoxicity in developing zebrafish.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107230"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of microplastic accumulation in aquatic environment: Physiological, eco-toxicological, immunological, and neurotoxic effects","authors":"Eram Rashid ,&nbsp;Syed Makhdoom Hussain ,&nbsp;Shafaqat Ali ,&nbsp;Muhammad Munir ,&nbsp;Abdul Ghafoor ,&nbsp;Ebru Yilmaz ,&nbsp;Mohammed Ali Alshehri ,&nbsp;Danish Riaz ,&nbsp;Adan Naeem ,&nbsp;Eman Naeem","doi":"10.1016/j.aquatox.2024.107232","DOIUrl":"10.1016/j.aquatox.2024.107232","url":null,"abstract":"<div><div>The presence of microplastics (MPs) in aquatic ecosystem has become a pressing global concern. MPs pose a significant threat to aquatic ecosystems, with devastating consequences for both aquatic life and human health. Notably, freshwater ecosystems are particularly vulnerable to MPs pollution. MPs, characterized by their small size (&lt; 5 mm), have emerged as a ubiquitous environmental pollutant. They exhibit diverse characteristics, including varying sizes, forms, polymer types, and colors. Two distinct categories of MPs exist: primary and secondary. Primary MPs are incorporated into industrial hard materials, cosmetics, and hand cleaners, whereas secondary MPs result from the breakdown of larger plastic products in both terrestrial and marine environments. They enter the environment through various sources, such as household products, clothing, industrial activities, sewage waste and plastic degradation. Aquatic organisms ingest these contaminants, facilitating the transfer of MPs into the food chain and potentially causing severe health problems. This review delves into the bioaccumulation of MPs in fish, highlighting the eco-toxicological, neurological and immunological effects. This review provides an in-depth analysis of innovative solutions for MPs removal and reduction. Finally, we delineate evidence-based strategies to mitigate impacts of MPs, offering valuable insights to inform policy formulations and accelerate the development of sustainable plastic technologies.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107232"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CLSSATP: Contrastive learning and self-supervised learning model for aquatic toxicity prediction","authors":"Ye Lin ,&nbsp;Xin Yang ,&nbsp;Mingxuan Zhang ,&nbsp;Jinyan Cheng ,&nbsp;Hai Lin ,&nbsp;Qi Zhao","doi":"10.1016/j.aquatox.2025.107244","DOIUrl":"10.1016/j.aquatox.2025.107244","url":null,"abstract":"<div><div>As compound concentrations in aquatic environments increase, the habitat degradation of aquatic organisms underscores the growing importance of studying the impact of chemicals on diverse aquatic populations. Understanding the potential impacts of different chemical substances on different species is a necessary requirement for protecting the environment and ensuring sustainable human development. In this regard, deep learning methods offer significant advantages over traditional experimental approaches in terms of cost, accuracy, and generalization ability. This research introduces CLSSATP, an efficient contrastive self-supervised learning deep neural network prediction model for organic toxicity. The model integrates two modules, a self-supervised learning module using molecular fingerprints for representation, and a contrastive learning module utilizing molecular graphs. Through dual-perspective learning, the model gains clear insights into the structural and property relationships of molecules. The experiment results indicate that our model outperforms comparative methods, demonstrating the effectiveness of our proposed architecture. Moreover, ablation experiments show that the self-supervised module and contrastive learning module respectively provide average performance improvements of 9.43 % and 10.98 % to CLSSATP. Furthermore, by visualizing the representations of our model, we observe that it correctly identifies the substructures that determine the molecular properties, granting itself with interpretability. In conclusion, CLSSATP offers a novel and effective perspective for future research in aquatic toxicity assessment. All of codes and datasets are freely available online at <span><span>https://github.co</span><svg><path></path></svg></span><span><span><em>m/z</em></span><svg><path></path></svg></span><span><span>haoqi106/CLSSATP</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107244"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single and combined chronic toxicity of cadmium and titanium dioxide nanoparticles in Aquarana catesbeiana (Anura: Ranidae) tadpoles","authors":"Carolina Arantes de Moraes ,&nbsp;Fagner Neves Oliveira ,&nbsp;Gabriel Qualhato ,&nbsp;Pedro Vale de Azevedo Brito ,&nbsp;Karina Simões ,&nbsp;Thiago Lopes Rocha ,&nbsp;Raquel Fernanda Salla ,&nbsp;Lucélia Gonçalves Vieira","doi":"10.1016/j.aquatox.2025.107246","DOIUrl":"10.1016/j.aquatox.2025.107246","url":null,"abstract":"<div><div>The rising production and improper disposal of titanium dioxide nanoparticles (TiO₂ NPs) into aquatic systems present considerable environmental challenges, especially when these particles interact with other contaminants such as cadmium (Cd). Thus, the current study aimed to evaluate the potential toxic effects on the gills, chondrocranium, body growth, and mortality of <em>Aquarana catesbeiana</em> tadpoles. The tadpoles were exposed to environmentally relevant concentrations of TiO₂ NPs (10 µg L^-1), and CdCl₂ (10 µg L^-1), both individually and in combination, for 30 days (chronic exposure), along with a control group. Our results indicate that the co-exposure to TiO₂ NPs and Cd induced a higher mortality rate. In the gills, TiO₂ NPs led to epithelial simplification, while Cd exposure resulted in stratified epithelium formation. Additionally, there were notable changes in the index of degenerative alterations for the co-exposed group and the overall organ index for the groups exposed to Cd and the mixture. The viscerocranium showed significant malformations in the ceratobranchials and reticular processes, indicating the mixture's toxicological potential during the skeletal system's development. Morphometric analysis also revealed reduced body length and abnormal body ratios in tadpoles from the co-exposure group. In conclusion, TiO₂ NPs and Cd, both alone and in combination, exhibit toxicological effects in <em>A. catesbeiana</em> tadpoles, indicating a potential ecological risk associated with releasing these contaminants into aquatic environments.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"279 ","pages":"Article 107246"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}