Intestinal microbiota and metabolomics reveal the intestinal damage caused by imidacloprid in loaches (Misgurnus anguillicaudatus)

IF 4.3 2区 环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY
Yingbing Su , Yongxi Huang , Pupu Yan , Jiali Liu , Man Liu , Jun Zhu , Yinuo Gong , Haishan Chen , Liwei Guo
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Abstract

Aim of this research was to assess the presence of imidacloprid residues in aquatic environments and their impact on the intestinal health of loaches. The concentrations of imidacloprid examined were 146 μg/L, 73 μg/L, 36.5 μg/L, 18.25 μg/L, 9.125 μg/L, and 4.5625 μg/L. Following a 28-day exposure period, alterations were observed in the intestinal barrier, inflammatory damage, antioxidant levels, intestinal microbiota, metabolite groups, and target genes of the loach. The exposure to imidacloprid caused damage to the intestinal barrier, infiltration of inflammatory cells, and downregulation of Occludin and ZO-1 protein expression in the intestinal tissue of loaches, while concurrently inducing oxidative stress and inflammatory damage. Furthermore, imidacloprid was found to diminish the diversity of gut microbiota while increasing the abundance of harmful bacterial taxa, specifically Proteobacteria and Aeromonas, thereby disrupting microbiota functionality. Metabolomic analysis revealed a significant increase in the number of differentially expressed genes associated with both upregulated and downregulated metabolites following exposure to imidacloprid, with these metabolites demonstrating dose-dependent effects. Additionally, interactions between metabolites and imidacloprid were identified through the regulation of CA, HSP90, and HSP60. This study highlights the detrimental effects of imidacloprid on loaches even at non-lethal concentrations.
肠道菌群和代谢组学揭示吡虫啉对泥鳅肠道的损害
本研究旨在评估吡虫啉在水生环境中的残留及其对泥鳅肠道健康的影响。吡虫啉检测浓度分别为146 μg/L、73 μg/L、36.5 μg/L、18.25 μg/L、9.125 μg/L、4.5625 μ L。在28天的暴露期后,观察到泥鳅肠道屏障、炎症损伤、抗氧化水平、肠道微生物群、代谢物群和靶基因的变化。吡虫啉暴露导致泥鳅肠屏障损伤,炎症细胞浸润,肠组织Occludin和ZO-1蛋白表达下调,同时诱导氧化应激和炎症损伤。此外,吡虫啉被发现会减少肠道微生物群的多样性,同时增加有害细菌分类群的丰度,特别是变形菌和气单胞菌,从而破坏微生物群的功能。代谢组学分析显示,暴露于吡虫啉后,与上调和下调代谢物相关的差异表达基因数量显著增加,这些代谢物表现出剂量依赖效应。此外,代谢物与吡虫啉之间的相互作用是通过CA、HSP90和HSP60的调控确定的。这项研究强调了即使在非致死浓度下吡虫啉对泥鳅的有害影响。
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来源期刊
Aquatic Toxicology
Aquatic Toxicology 环境科学-毒理学
CiteScore
7.10
自引率
4.40%
发文量
250
审稿时长
56 days
期刊介绍: Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.
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