Yingbing Su , Yongxi Huang , Pupu Yan , Jiali Liu , Man Liu , Jun Zhu , Yinuo Gong , Haishan Chen , Liwei Guo
{"title":"Intestinal microbiota and metabolomics reveal the intestinal damage caused by imidacloprid in loaches (Misgurnus anguillicaudatus)","authors":"Yingbing Su , Yongxi Huang , Pupu Yan , Jiali Liu , Man Liu , Jun Zhu , Yinuo Gong , Haishan Chen , Liwei Guo","doi":"10.1016/j.aquatox.2025.107533","DOIUrl":null,"url":null,"abstract":"<div><div>Aim of this research was to assess the presence of imidacloprid residues in aquatic environments and their impact on the intestinal health of loaches. The concentrations of imidacloprid examined were 146 μg/L, 73 μg/L, 36.5 μg/L, 18.25 μg/L, 9.125 μg/L, and 4.5625 μg/L. Following a 28-day exposure period, alterations were observed in the intestinal barrier, inflammatory damage, antioxidant levels, intestinal microbiota, metabolite groups, and target genes of the loach. The exposure to imidacloprid caused damage to the intestinal barrier, infiltration of inflammatory cells, and downregulation of Occludin and ZO-1 protein expression in the intestinal tissue of loaches, while concurrently inducing oxidative stress and inflammatory damage. Furthermore, imidacloprid was found to diminish the diversity of gut microbiota while increasing the abundance of harmful bacterial taxa, specifically <em>Proteobacteria</em> and <em>Aeromonas</em>, thereby disrupting microbiota functionality. Metabolomic analysis revealed a significant increase in the number of differentially expressed genes associated with both upregulated and downregulated metabolites following exposure to imidacloprid, with these metabolites demonstrating dose-dependent effects. Additionally, interactions between metabolites and imidacloprid were identified through the regulation of CA, HSP90, and HSP60. This study highlights the detrimental effects of imidacloprid on loaches even at non-lethal concentrations.</div></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":"287 ","pages":"Article 107533"},"PeriodicalIF":4.3000,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquatic Toxicology","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0166445X25002978","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Aim of this research was to assess the presence of imidacloprid residues in aquatic environments and their impact on the intestinal health of loaches. The concentrations of imidacloprid examined were 146 μg/L, 73 μg/L, 36.5 μg/L, 18.25 μg/L, 9.125 μg/L, and 4.5625 μg/L. Following a 28-day exposure period, alterations were observed in the intestinal barrier, inflammatory damage, antioxidant levels, intestinal microbiota, metabolite groups, and target genes of the loach. The exposure to imidacloprid caused damage to the intestinal barrier, infiltration of inflammatory cells, and downregulation of Occludin and ZO-1 protein expression in the intestinal tissue of loaches, while concurrently inducing oxidative stress and inflammatory damage. Furthermore, imidacloprid was found to diminish the diversity of gut microbiota while increasing the abundance of harmful bacterial taxa, specifically Proteobacteria and Aeromonas, thereby disrupting microbiota functionality. Metabolomic analysis revealed a significant increase in the number of differentially expressed genes associated with both upregulated and downregulated metabolites following exposure to imidacloprid, with these metabolites demonstrating dose-dependent effects. Additionally, interactions between metabolites and imidacloprid were identified through the regulation of CA, HSP90, and HSP60. This study highlights the detrimental effects of imidacloprid on loaches even at non-lethal concentrations.
期刊介绍:
Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems.
Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants
The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.