Validation of an in vitro bioassay using C6/36 insect cells as a model for evaluating toxicity of aquatic contaminants to invertebrates

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-07-04 DOI:10.1016/j.aquatox.2025.107481

Matthew Johnson , Kimberly A. Finlayson , Jason P. van de Merwe , Frederic D.L. Leusch

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引用次数: 0

Abstract

Aquatic invertebrates play an important role in ecosystem functioning and are commonly used for in vivo toxicity assessments of environmental contaminants. However, in vitro bioassays employing invertebrate cell lines remain underutilised, despite their potential to improve toxicity testing and reduce reliance on whole-organism assays. This study aimed to develop and validate a suite of in vitro bioassays using the Asian tiger mosquito (Aedes albopictus) larval cell line (C6/36) to assess the cytotoxicity, oxidative stress, and genotoxicity of 24 common environmental contaminants. Cytotoxic effects were observed in 71 % of tested chemicals, while 25 % induced oxidative stress, and 63 % showed measurable genotoxicity. A strong correlation (R² = 0.83) between in vitro cytotoxicity data and acute toxicity data previously reported for Daphnia spp. suggests that C6/36 cells may serve as a reliable surrogate for traditional in vivo invertebrate toxicity tests. These findings support the broader application of invertebrate cell lines for environmental risk assessment, offering a refined approach for evaluating the toxicological properties of aquatic contaminants, while potentially reducing reliance on animal testing.

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使用C6/36昆虫细胞作为评估水生污染物对无脊椎动物毒性模型的体外生物测定验证

水生无脊椎动物在生态系统功能中发挥着重要作用，通常用于环境污染物的体内毒性评估。然而，使用无脊椎动物细胞系的体外生物测定法仍未得到充分利用，尽管它们有可能改善毒性测试并减少对整个生物体测定的依赖。本研究旨在利用亚洲虎蚊（白纹伊蚊）幼虫细胞系C6/36，建立并验证一套体外生物检测方法，以评估24种常见环境污染物的细胞毒性、氧化应激和遗传毒性。在71%的测试化学物质中观察到细胞毒性作用，而25%诱导氧化应激，63%显示可测量的遗传毒性。体外细胞毒性数据与先前报道的水蚤急性毒性数据之间有很强的相关性（R² = 0.83），这表明C6/36细胞可以作为传统的体内无脊椎动物毒性试验的可靠替代品。这些发现支持了无脊椎动物细胞系在环境风险评估中的广泛应用，为评估水生污染物的毒理学特性提供了一种完善的方法，同时有可能减少对动物试验的依赖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.