The impact of an early exposure to 17α-ethynylestradiol on the physiology of the three-spined stickleback (Gasterosteus aculeatus) under current and future climatic scenarios

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-08-05 DOI:10.1016/j.aquatox.2025.107528

Jimmy Devergne , Arianna Servili , Sylvain Jodet , Titouan Brandicourt , Christophe Lebigre , Sophie Collet , Olivier Mouchel , Marie Lou Fleury , Sabine Roussel , Véronique Loizeau

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引用次数: 0

Abstract

Ocean warming and acidification are climate change related drivers that impact the physiology of marine organisms and their ability to cope with future environments. Marine ecosystems are also facing pollution from an ever-growing diversity of chemical contaminants, including endocrine disruptors. A common example is the 17α-ethynylestradiol (EE2), which can affect the endocrine regulation of fish and hence potentially impact their fitness. Thus, fish have to cope to multiple climatic and chemical stresses that can interact, influencing the overall impact on fish physiology. In this study, we investigated whether the direct and carry-over effect of early exposure to EE2 (15 ng.L⁻¹; one month during embryo-larval development) are modulated by the RCP8.5 scenario (+3°C; -0.4 pH unit). Five months post-contamination, we measured survival, growth and reproductive axis of prepubertal sticklebacks. Our findings revealed that the survival of juveniles, when exposed to EE2 during early development, is reduced under Current but not RCP8.5 scenario. Furthermore, under RCP8.5-EE2, a significantly lower body length was observed. Sex and tissue specific responses in terms of the expression profiles of genes related to development and sexual maturation was reported. Interestingly, significant interaction between RCP8.5 and EE2 was observed for the expression of ovarian aromatase (cyp19a1a), suggesting a long-lasting estrogenic effect under RCP8.5 scenario. Additionally, the skewed sex ratios and the presence of intersex individuals in both scenarios early exposed to EE2 suggested a feminization due to EE2, which could potentially disrupt sexual maturation and future reproduction. Hence, the early EE2 exposure had carry-over physiological effects on sticklebacks, and these effects can be modulated by the climate scenario. This underscores the importance of conducting long-term multi-stress studies to comprehensively understand the vulnerability on fish populations in future environments.

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早期暴露于17α-乙炔雌醇对三棘棘鱼（Gasterosteus aculeatus）生理在当前和未来气候情景下的影响

海洋变暖和酸化是与气候变化相关的驱动因素，影响海洋生物的生理机能及其应对未来环境的能力。海洋生态系统也面临着日益多样化的化学污染物的污染，包括内分泌干扰物。一个常见的例子是17α-乙炔雌醇（EE2），它可以影响鱼类的内分泌调节，从而潜在地影响它们的健康。因此，鱼类必须应对多种气候和化学压力，这些压力可以相互作用，影响对鱼类生理的总体影响。在这项研究中，我们调查了早期暴露于EE2 (15 ng.L−1；RCP8.5情景(+3°C；-0.4 pH单位)。污染后5个月，我们测量了青春期前棘鱼的生存、生长和生殖轴。我们的研究结果表明，在当前而非RCP8.5情景下，幼年鱼在发育早期暴露于EE2会降低其存活率。RCP8.5-EE2处理下体长显著降低。根据与发育和性成熟相关的基因表达谱的性别和组织特异性反应进行了报道。有趣的是，RCP8.5和EE2对卵巢芳香化酶（cyp19a1a）的表达有显著的相互作用，表明在RCP8.5条件下具有持久的雌激素效应。此外，在早期暴露于EE2的两种情况下，性别比例失衡和双性个体的存在表明，EE2可能导致雌性化，这可能会破坏性成熟和未来的繁殖。因此，早期的EE2暴露对棘鱼具有携带性生理影响，这些影响可以通过气候情景来调节。这就强调了开展长期多重胁迫研究以全面了解未来环境对鱼类种群的脆弱性的重要性。

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.