Structure最新文献_第2页

Antiterminator LoaP loads onto RNA to chase a runaway RNA polymerase 反终结者 LoaP 加载到 RNA 上，追逐失控的 RNA 聚合酶

IF 5.7 2区生物学

Structure Pub Date : 2024-09-05 DOI: 10.1016/j.str.2024.07.024

Bing Wang, Irina Artsimovitch

引用次数: 0

Crystal structure of lipase from Pseudomonas aeruginosa reveals an unusual catalytic triad conformation. 铜绿假单胞菌脂肪酶的晶体结构揭示了不寻常的催化三元构象。

IF 4.4 2区生物学

Structure Pub Date : 2024-09-05 Epub Date: 2024-07-17 DOI: 10.1016/j.str.2024.06.014

Gang Xu, Hua Guo, Zhonglang Yu, Shulin Wang, Dandan Shen, Lirong Yang, Jianping Wu, Binbin Chen, Haoran Yu

引用次数: 0

Structural review of SARS-CoV-2 antiviral targets SARS-CoV-2 抗病毒靶标的结构回顾

IF 5.7 2区生物学

Structure Pub Date : 2024-09-05 DOI: 10.1016/j.str.2024.08.005

Wen Cui, Yinkai Duan, Yan Gao, Wei Wang, Haitao Yang

引用次数: 0

Binding of steroid substrates reveals the key to the productive transition of the cytochrome P450 OleP. 类固醇底物的结合揭示了细胞色素 P450 OleP 生产转换的关键。

IF 4.4 2区生物学

Structure Pub Date : 2024-09-05 Epub Date: 2024-07-05 DOI: 10.1016/j.str.2024.06.005

Antonella Costanzo, Francesca Fata, Ida Freda, Maria Laura De Sciscio, Elena Gugole, Giovanni Bulfaro, Matteo Di Renzo, Luca Barbizzi, Cécile Exertier, Giacomo Parisi, Marco D'Abramo, Beatrice Vallone, Carmelinda Savino, Linda Celeste Montemiglio

{"title":"Binding of steroid substrates reveals the key to the productive transition of the cytochrome P450 OleP.","authors":"Antonella Costanzo, Francesca Fata, Ida Freda, Maria Laura De Sciscio, Elena Gugole, Giovanni Bulfaro, Matteo Di Renzo, Luca Barbizzi, Cécile Exertier, Giacomo Parisi, Marco D'Abramo, Beatrice Vallone, Carmelinda Savino, Linda Celeste Montemiglio","doi":"10.1016/j.str.2024.06.005","DOIUrl":"10.1016/j.str.2024.06.005","url":null,"abstract":"OleP is a bacterial cytochrome P450 involved in oleandomycin biosynthesis as it catalyzes regioselective epoxidation on macrolide intermediates. OleP has recently been reported to convert lithocholic acid (LCA) into murideoxycholic acid through a highly regioselective reaction and to unspecifically hydroxylate testosterone (TES). Since LCA and TES mainly differ by the substituent group at the C17, here we used X-ray crystallography, equilibrium binding assays, and molecular dynamics simulations to investigate the molecular basis of the diverse reactivity observed with the two steroids. We found that the differences in the structure of TES and LCA affect the capability of these molecules to directly form hydrogen bonds with N-terminal residues of OleP internal helix I. The establishment of these contacts, by promoting the bending of helix I, fosters an efficient trigger of the open-to-closed structural transition that occurs upon substrate binding to OleP and contributes to the selectivity of the subsequent monooxygenation reaction.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA dynamics from experimental and computational approaches 从实验和计算方法看 RNA 动力学

IF 5.7 2区生物学

Structure Pub Date : 2024-09-05 DOI: 10.1016/j.str.2024.07.019

Giovanni Bussi, Massimiliano Bonomi, Paraskevi Gkeka, Michael Sattler, Hashim M. Al-Hashimi, Pascal Auffinger, Maria Duca, Yann Foricher, Danny Incarnato, Alisha N. Jones, Serdal Kirmizialtin, Miroslav Krepl, Modesto Orozco, Giulia Palermo, Samuela Pasquali, Loïc Salmon, Harald Schwalbe, Eric Westhof, Martin Zacharias

引用次数: 0

Structure of calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A 与 PI4KA 结合的钙神经蛋白的结构揭示了 PI4KA 和 FAM126A 的双重界面

IF 5.7 2区生物学

Structure Pub Date : 2024-08-30 DOI: 10.1016/j.str.2024.08.007

Alexandria L. Shaw, Sushant Suresh, Matthew A.H. Parson, Noah J. Harris, Meredith L. Jenkins, Calvin K. Yip, John E. Burke

{"title":"Structure of calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A","authors":"Alexandria L. Shaw, Sushant Suresh, Matthew A.H. Parson, Noah J. Harris, Meredith L. Jenkins, Calvin K. Yip, John E. Burke","doi":"10.1016/j.str.2024.08.007","DOIUrl":"https://doi.org/10.1016/j.str.2024.08.007","url":null,"abstract":"Phosphatidylinositol 4-kinase alpha (PI4KA) maintains the phosphatidylinositol 4-phosphate (PI4P) and phosphatidylserine pools of the plasma membrane. A key regulator of PI4KA is its association into a complex with TTC7 and FAM126 proteins. This complex can be regulated by the CNAβ1 isoform of the phosphatase calcineurin. We previously identified that CNAβ1 directly binds to FAM126A. Here, we report a cryoelectron microscopic (cryo-EM) structure of a truncated PI4KA complex bound to calcineurin, revealing a unique direct interaction between PI4KA and calcineurin. Hydrogen deuterium exchange mass spectrometry (HDX-MS) and computational analysis show that calcineurin forms a complex with an evolutionarily conserved IKISVT sequence in PI4KA’s horn domain. We also characterized conserved LTLT and PSISIT calcineurin binding sequences in the C terminus of FAM126A. These dual sites in PI4KA and FAM126A are both in close proximity to phosphorylation sites in the PI4KA complex, suggesting key roles of calcineurin-regulated phosphosites in PI4KA regulation. This work reveals novel insight into how calcineurin can regulate PI4KA activity.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142102027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism 一种水底植物色素组氨酸激酶的低温电子显微镜结构揭示了一种独特的光依赖性激活机制

IF 5.7 2区生物学

Structure Pub Date : 2024-08-30 DOI: 10.1016/j.str.2024.08.008

Szabolcs Bódizs, Petra Mészáros, Lukas Grunewald, Heikki Takala, Sebastian Westenhoff

{"title":"Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism","authors":"Szabolcs Bódizs, Petra Mészáros, Lukas Grunewald, Heikki Takala, Sebastian Westenhoff","doi":"10.1016/j.str.2024.08.008","DOIUrl":"https://doi.org/10.1016/j.str.2024.08.008","url":null,"abstract":"Phytochromes are photoreceptor proteins in plants, fungi, and bacteria. They can adopt two photochromic states with differential biochemical responses. The structural changes transducing the signal from the chromophore to the biochemical output modules are poorly understood due to challenges in capturing structures of the dynamic, full-length protein. Here, we present cryoelectron microscopy (cryo-EM) structures of the phytochrome from Pseudomonas aeruginosa (PaBphP) in its resting (Pfr) and photoactivated (Pr) state. The kinase-active Pr state has an asymmetric, dimeric structure, whereas the kinase-inactive Pfr state opens up. This behavior is different from other known phytochromes and we explain it with the unusually short connection between the photosensory and output modules. Multiple sequence alignment of this region suggests evolutionary optimization for different modes of signal transduction in sensor proteins. The results establish a new mechanism for light-sensing by phytochrome histidine kinases and provide input for the design of optogenetic phytochrome variants.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142101070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural insights into the convergent evolution of sulfoxide synthase EgtB-IV, an ergothioneine-biosynthetic homolog of ovothiol synthase OvoA 亚砜合成酶EgtB-IV（麦角硫因生物合成同源物卵硫醇合成酶OvoA）趋同进化的结构见解

IF 5.7 2区生物学

Structure Pub Date : 2024-08-30 DOI: 10.1016/j.str.2024.08.006

Kendra A. Ireland, Chase M. Kayrouz, Marissa L. Abbott, Mohammad R. Seyedsayamdost, Katherine M. Davis

{"title":"Structural insights into the convergent evolution of sulfoxide synthase EgtB-IV, an ergothioneine-biosynthetic homolog of ovothiol synthase OvoA","authors":"Kendra A. Ireland, Chase M. Kayrouz, Marissa L. Abbott, Mohammad R. Seyedsayamdost, Katherine M. Davis","doi":"10.1016/j.str.2024.08.006","DOIUrl":"https://doi.org/10.1016/j.str.2024.08.006","url":null,"abstract":"Non-heme iron-dependent sulfoxide/selenoxide synthases (NHISS) constitute a unique metalloenzyme class capable of installing a C–S/Se bond onto histidine to generate thio/selenoimidazole antioxidants, such as ergothioneine and ovothiol. These natural products are increasingly recognized for their health benefits. Among associated ergothioneine-biosynthetic enzymes, type IV EgtBs stand out, as they exhibit low sequence similarity with other EgtB subfamilies due to their recent divergence from the ovothiol-biosynthetic enzyme OvoA. Herein, we present crystal structures of two representative EgtB-IV enzymes, offering insights into the basis for this evolutionary convergence and enhancing our understanding of NHISS active site organization more broadly. The ability to interpret how key residues modulate substrate specificity and regioselectivity has implications for downstream identification of divergent reactivity within the NHISS family. To this end, we identify a previously unclassified clade of OvoA-like enzymes with a seemingly hybrid set of characteristics, suggesting they may represent an evolutionary intermediate between OvoA and EgtB-IV.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142101069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel, tightly structurally related N-myristoyltransferase inhibitors display equally potent yet distinct inhibitory mechanisms 结构紧密相关的新型 N-肉豆蔻酰基转移酶抑制剂显示出同等效力但不同的抑制机制

IF 5.7 2区生物学

Structure Pub Date : 2024-08-28 DOI: 10.1016/j.str.2024.08.001

Frédéric Rivière, Cyril Dian, Rémi F. Dutheil, Paul Monassa, Carmela Giglione, Thierry Meinnel

引用次数: 0

Unveiling the double-edged sword: SOD1 trimers possess tissue-selective toxicity and bind septin-7 in motor neuron-like cells 揭开双刃剑的面纱：SOD1 三聚体具有组织选择性毒性，并能与运动神经元样细胞中的 septin-7 结合

IF 5.7 2区生物学

Structure Pub Date : 2024-08-28 DOI: 10.1016/j.str.2024.08.002

Esther Sue Choi, Brianna Hnath, Congzhou Mike Sha, Nikolay V. Dokholyan

{"title":"Unveiling the double-edged sword: SOD1 trimers possess tissue-selective toxicity and bind septin-7 in motor neuron-like cells","authors":"Esther Sue Choi, Brianna Hnath, Congzhou Mike Sha, Nikolay V. Dokholyan","doi":"10.1016/j.str.2024.08.002","DOIUrl":"https://doi.org/10.1016/j.str.2024.08.002","url":null,"abstract":"Misfolded species of superoxide dismutase 1 (SOD1) are associated with increased death in amyotrophic lateral sclerosis (ALS) models compared to insoluble protein aggregates. The mechanism by which structurally independent SOD1 trimers cause cellular toxicity is unknown but may drive disease pathology. Here, we uncovered the SOD1 trimer interactome—a map of potential tissue-selective protein-binding partners in the brain, spinal cord, and skeletal muscle. We identified binding partners and key pathways associated with SOD1 trimers and found that trimers may affect normal cellular functions such as dendritic spine morphogenesis and synaptic function in the central nervous system and cellular metabolism in skeletal muscle. We discovered SOD1 trimer-selective enrichment of genes. We performed detailed computational and biochemical characterization of SOD1 trimer protein binding for septin-7. Our investigation highlights key proteins and pathways within distinct tissues, revealing a plausible intersection of genetic and pathophysiological mechanisms in ALS through interactions involving SOD1 trimers.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142085757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0