Structure最新文献_第3页

Insights into the distinct membrane targeting mechanisms of WDR91 family proteins 深入了解 WDR91 家族蛋白的不同膜靶向机制

IF 5.7 2区生物学

Structure Pub Date : 2024-10-18 DOI: 10.1016/j.str.2024.09.023

Xinli Ma, Jian Li, Nan Liu, Surajit Banerjee, Xiaotong Hu, Xiaoyu Wang, Jianshu Dong, Kangdong Liu, Chonglin Yang, Zigang Dong

引用次数: 0

Structural insights into the DNA-binding mechanism of BCL11A: The integral role of ZnF6 对 BCL11A DNA 结合机制的结构性认识：ZnF6 的重要作用

IF 5.7 2区生物学

Structure Pub Date : 2024-10-17 DOI: 10.1016/j.str.2024.09.022

Thibault Viennet, Maolu Yin, Abhilash Jayaraj, Woojin Kim, Zhen-Yu J. Sun, Yuko Fujiwara, Kevin Zhang, Davide Seruggia, Hyuk-Soo Seo, Sirano Dhe-Paganon, Stuart H. Orkin, Haribabu Arthanari

引用次数: 0

ATTRv-V30M amyloid fibrils from heart and nerves exhibit structural homogeneity 来自心脏和神经的 ATTRv-V30M 淀粉样蛋白纤维在结构上具有同质性

IF 5.7 2区生物学

Structure Pub Date : 2024-10-17 DOI: 10.1016/j.str.2024.09.021

Binh An Nguyen, Shumaila Afrin, Anna Yakubovska, Virender Singh, Rose Pedretti, Parker Bassett, Maja Pekala, Jaime Vaquer Alicea, Peter Kunach, Lanie Wang, Andrew Lemoff, Barbara Kluve-Beckerman, Lorena Saelices

{"title":"ATTRv-V30M amyloid fibrils from heart and nerves exhibit structural homogeneity","authors":"Binh An Nguyen, Shumaila Afrin, Anna Yakubovska, Virender Singh, Rose Pedretti, Parker Bassett, Maja Pekala, Jaime Vaquer Alicea, Peter Kunach, Lanie Wang, Andrew Lemoff, Barbara Kluve-Beckerman, Lorena Saelices","doi":"10.1016/j.str.2024.09.021","DOIUrl":"https://doi.org/10.1016/j.str.2024.09.021","url":null,"abstract":"Amyloidogenic transthyretin (ATTR) amyloidosis is a systemic disease characterized by the deposition of amyloid fibrils made of transthyretin. Transthyretin is primarily produced in tetrameric form by the liver, but also by retinal epithelium and choroid plexus. The deposition of these fibrils in the myocardium and peripheral nerves causes cardiomyopathies and neuropathies, respectively. Using cryoelectron microscopy (cryo-EM), we investigated fibrils extracted from cardiac and nerve tissues of an ATTRv-V30M patient. We found consistent fibril structures from both tissues, similar to cardiac fibrils previously described, but different from vitreous humor fibrils of the same genotype. Our findings, along with previous ATTR fibrils structural studies, suggest a uniform fibrillar architecture across different tissues when transthyretin originates from the liver. This study advances our understanding of how deposition and production sites influence fibril structure in ATTRv-V30M amyloidosis.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribosome-inactivation by a class of widely distributed C-tail anchored membrane proteins 一类广泛分布的 C 尾锚定膜蛋白的核糖体失活作用

IF 5.7 2区生物学

Structure Pub Date : 2024-10-16 DOI: 10.1016/j.str.2024.09.019

Robert Karari Njenga, Julian Boele, Friedel Drepper, Kasturica Sinha, Eirini Marouda, Pitter F. Huesgen, Crysten Blaby-Haas, Hans-Georg Koch

{"title":"Ribosome-inactivation by a class of widely distributed C-tail anchored membrane proteins","authors":"Robert Karari Njenga, Julian Boele, Friedel Drepper, Kasturica Sinha, Eirini Marouda, Pitter F. Huesgen, Crysten Blaby-Haas, Hans-Georg Koch","doi":"10.1016/j.str.2024.09.019","DOIUrl":"https://doi.org/10.1016/j.str.2024.09.019","url":null,"abstract":"Ribosome hibernation is a commonly used strategy that protects ribosomes under unfavorable conditions and regulates developmental processes. Multiple ribosome-hibernation factors have been identified in all domains of life, but due to their structural diversity and the lack of a common inactivation mechanism, it is currently unknown how many different hibernation factors exist. Here, we show that the YqjD/ElaB/YgaM paralogs, initially discovered as membrane-bound ribosome binding proteins in E. coli, constitute an abundant class of ribosome-hibernating proteins, which are conserved across all proteobacteria and some other bacterial phyla. Our data demonstrate that they inhibit in vitro protein synthesis by interacting with the 50S ribosomal subunit. In vivo cross-linking combined with mass spectrometry revealed their specific interactions with proteins surrounding the ribosomal tunnel exit and even their penetration into the ribosomal tunnel. Thus, YqjD/ElaB/YgaM inhibit translation by blocking the ribosomal tunnel and thus mimic the activity of antimicrobial peptides and macrolide antibiotics.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural requirements for the specific binding of CRABP2 to cyclin D3 CRABP2 与细胞周期蛋白 D3 特异性结合的结构要求

IF 5.7 2区生物学

Structure Pub Date : 2024-10-16 DOI: 10.1016/j.str.2024.09.020

Martyna W. Pastok, Charles W.E. Tomlinson, Shannon Turberville, Abbey M. Butler, Arnaud Baslé, Martin E.M. Noble, Jane A. Endicott, Ehmke Pohl, Natalie J. Tatum

引用次数: 0

PARG inhibition induces nuclear aggregation of PARylated PARP1 PARG 抑制可诱导 PARylated PARP1 的核聚集

IF 5.7 2区生物学

Structure Pub Date : 2024-10-14 DOI: 10.1016/j.str.2024.09.006

Sateja Paradkar, Julia Purcell, Annie Cui, Sam Friedman, Katelyn J. Noronha, Matthew A. Murray, Ranjini K. Sundaram, Ranjit S. Bindra, Ryan B. Jensen

引用次数: 0

Structural basis for the ligand promiscuity of the hydroxamate siderophore binding protein FtsB from Streptococcus pyogenes 化脓性链球菌中的羟基氨基甲酸酯苷元结合蛋白 FtsB 的配体杂合性的结构基础

IF 5.7 2区生物学

Structure Pub Date : 2024-10-11 DOI: 10.1016/j.str.2024.09.018

Jorge Fernandez-Perez, Akinobu Senoo, Jose M.M. Caaveiro, Makoto Nakakido, Susana de Vega, Ichiro Nakagawa, Kouhei Tsumoto

{"title":"Structural basis for the ligand promiscuity of the hydroxamate siderophore binding protein FtsB from Streptococcus pyogenes","authors":"Jorge Fernandez-Perez, Akinobu Senoo, Jose M.M. Caaveiro, Makoto Nakakido, Susana de Vega, Ichiro Nakagawa, Kouhei Tsumoto","doi":"10.1016/j.str.2024.09.018","DOIUrl":"https://doi.org/10.1016/j.str.2024.09.018","url":null,"abstract":"Pathogenic bacteria must secure the uptake of nutritional metals such as iron for their growth, making their import systems attractive targets for the development of new antimicrobial modalities. In the pathogenic bacterium Streptococcus pyogenes, the iron uptake system FtsABCD transports iron encapsulated by siderophores of the hydroxamate class. However, the inability of S. pyogenes to produce these metabolites makes the biological and clinical relevance of this route unresolved. Herein, we demonstrated that the periplasmic binding protein FtsB recognizes not only the hydroxamate siderophore ferrichrome, as previously documented, but also ferrioxamine E (FOE), ferrioxamine B (FOB), and bisucaberin (BIS), each of them with high affinity (nM level). Up to seven aromatic residues in the binding pocket accommodate the variable backbones of the different siderophores through CH-π interactions, explaining ligand promiscuity. Collectively, our observations revealed how S. pyogenes exploits the diverse xenosiderophores produced by other microorganisms as iron sources to secure this precious nutrient.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural dissection of the CMP-pseudaminic acid synthetase, PseF CMP 伪氨基酸合成酶 PseF 的结构剖析

IF 5.7 2区生物学

Structure Pub Date : 2024-10-10 DOI: 10.1016/j.str.2024.09.017

Tessa Keenan, Andrew R. Cowan, Emily K.P. Flack, Natasha E. Hatton, Abigail J. Walklett, Gavin H. Thomas, Glyn R. Hemsworth, Martin A. Fascione

引用次数: 0

PROTAC-mediated activation, rather than degradation, of a nuclear receptor reveals complex ligand-receptor interaction network PROTAC 介导的核受体激活而非降解揭示了复杂的配体-受体相互作用网络

IF 5.7 2区生物学

Structure Pub Date : 2024-10-09 DOI: 10.1016/j.str.2024.09.016

Andrew D. Huber, Wenwei Lin, Shyaron Poudel, Darcie J. Miller, Taosheng Chen

{"title":"PROTAC-mediated activation, rather than degradation, of a nuclear receptor reveals complex ligand-receptor interaction network","authors":"Andrew D. Huber, Wenwei Lin, Shyaron Poudel, Darcie J. Miller, Taosheng Chen","doi":"10.1016/j.str.2024.09.016","DOIUrl":"https://doi.org/10.1016/j.str.2024.09.016","url":null,"abstract":"Proteolysis-targeting chimeras (PROTACs) are heterobifunctional molecules containing a ligand for a protein of interest linked to an E3 ubiquitin ligase ligand that induce protein degradation through E3 recruitment to the target protein. Small changes in PROTAC linkers can have drastic consequences, including loss of degradation activity, but the structural mechanisms governing such changes are unclear. To study this phenomenon, we screened PROTACs of diverse targeting modalities and identified dTAG-13 as an activator of the xenobiotic-sensing pregnane X receptor (PXR), which promiscuously binds various ligands. Characterization of dTAG-13 analogs and precursors revealed interplay between the PXR-binding moiety, linker, and E3 ligand that altered PXR activity without inducing degradation. A crystal structure of PXR ligand binding domain bound to a precursor ligand showed ligand-induced binding pocket distortions and a linker-punctured tunnel to the protein exterior at a region incompatible with E3 complex formation, highlighting the effects of linker environment on PROTAC activity.","PeriodicalId":22168,"journal":{"name":"Structure","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142385777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-EM structures of Clostridium perfringens enterotoxin bound to its human receptor, claudin-4 产气荚膜梭菌肠毒素与其人类受体 Claudin-4 结合的冷冻电子显微镜结构

IF 5.7 2区生物学

Structure Pub Date : 2024-10-08 DOI: 10.1016/j.str.2024.09.015

Sewwandi S. Rathnayake, Satchal K. Erramilli, Anthony A. Kossiakoff, Alex J. Vecchio

引用次数: 0