Structure最新文献

A tale of detergent tails: GPCR activation beyond ligands

IF 5.7 2区生物学

Structure Pub Date : 2025-04-03 DOI: 10.1016/j.str.2025.03.005

Eugene Agyemang, Rajan Lamichhane

引用次数: 0

Spy-ing on nucleic acids: Atomic resolution of the S. pyogenes CRISPR-Cas9 surveillance state

IF 5.7 2区生物学

Structure Pub Date : 2025-04-03 DOI: 10.1016/j.str.2025.03.001

Alexa L. Knight, George P. Lisi

引用次数: 0

Structure of the Staphylococcus aureus bacteriophage 80α neck shows details of the DNA, tail completion protein, and tape measure protein

IF 5.7 2区生物学

Structure Pub Date : 2025-04-01 DOI: 10.1016/j.str.2025.03.007

James L. Kizziah, Amarshi Mukherjee, Laura K. Parker, Terje Dokland

引用次数: 0

Disulfide-stabilized diabodies enable near-atomic cryo-EM imaging of small proteins: A case study of the bacterial Na+/citrate symporter CitS

IF 5.7 2区生物学

Structure Pub Date : 2025-03-31 DOI: 10.1016/j.str.2025.03.006

Subin Kim, Ji Won Kim, Jun Gyou Park, Sang Soo Lee, Seung Hun Choi, Jie-Oh Lee, Mi Sun Jin

引用次数: 0

ATP binding controls the molecular function of bacterial MutS2 by mediating closure of the dimeric clamp structure

IF 5.7 2区生物学

Structure Pub Date : 2025-03-28 DOI: 10.1016/j.str.2025.03.003

Kenji Fukui, Takeshi Murakawa, Nobumasa Hino, Naoyuki Kondo, Takato Yano

引用次数: 0

Structural basis for the constitutive activity of the melanocortin receptor family

IF 5.7 2区生物学

Structure Pub Date : 2025-03-28 DOI: 10.1016/j.str.2025.03.004

Wenbo Feng, Qingtong Zhou, Chang Zheng, Dehua Yang, Ming-Wei Wang

引用次数: 0

A mesophilic Argonaute from Clostridium formicaceticum with efficient DNA cleavage activity guided by small DNA

IF 5.7 2区生物学

Structure Pub Date : 2025-03-28 DOI: 10.1016/j.str.2025.03.002

Jianrui Li, Meixia Yu, Zhijia Yang, Yueheng Zhou, Yunpeng Teng, Zijian Wang, Jian Chen, Jinsheng Lai, Beibei Xin

{"title":"A mesophilic Argonaute from Clostridium formicaceticum with efficient DNA cleavage activity guided by small DNA","authors":"Jianrui Li, Meixia Yu, Zhijia Yang, Yueheng Zhou, Yunpeng Teng, Zijian Wang, Jian Chen, Jinsheng Lai, Beibei Xin","doi":"10.1016/j.str.2025.03.002","DOIUrl":"https://doi.org/10.1016/j.str.2025.03.002","url":null,"abstract":"We characterized a new Argonaute protein (pAgo), CfAgo, from the mesophilic bacterium Clostridium formicaceticum. CfAgo possesses DNA-guided DNA endonuclease activity and cleaves DNA targets at the canonical site. It is active from 28°C to 75°C and prefers DNA guides with a 5′-phosphate group and thymidine as the first nucleotide. Cleavage activity is reduced by single-nucleotide mismatches in the seed, central, and 3′-supplementary regions of guides, with stronger mismatch discrimination observed for 5′hydroxylated (5′OH) guides compared to 5′phosphorylated (5′P) guides. Moreover, structural analysis suggests that the MID domain of CfAgo is crucial for recognizing the 5′ guide and it influences the binding specificity. CfAgo catalyzes programmable cleavage of double-stranded DNA in AT-rich regions in the presence of Mn2+ and Mg2+ ions at appropriate salt concentrations. These properties could make CfAgo a promising tool for DNA manipulation such as nucleic acid detection and cleavage.","PeriodicalId":22168,"journal":{"name":"Structure","volume":"15 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structurally diverse C-terminal accessory domains in type I ABC importers reveal distinct regulatory mechanisms

IF 5.7 2区生物学

Structure Pub Date : 2025-03-24 DOI: 10.1016/j.str.2025.02.014

Evan R. Buechel, Valentina S. Dimitrova, Alexandra Karagiaridi, Lydia G. Kenney, Heather W. Pinkett

引用次数: 0

A structural perspective on the temperature dependent activity of enzymes

IF 5.7 2区生物学

Structure Pub Date : 2025-03-21 DOI: 10.1016/j.str.2025.02.013

Matthew J. McLeod, Sarah A.E. Barwell, Todd Holyoak, Robert E. Thorne

{"title":"A structural perspective on the temperature dependent activity of enzymes","authors":"Matthew J. McLeod, Sarah A.E. Barwell, Todd Holyoak, Robert E. Thorne","doi":"10.1016/j.str.2025.02.013","DOIUrl":"https://doi.org/10.1016/j.str.2025.02.013","url":null,"abstract":"Enzyme activity varies with temperature. Unlike small-molecule catalysts, the structural ensembles of enzymes can change substantially with temperature, but it is unclear how this modulates temperature dependent activity. Here, multi-temperature X-ray crystallography was used to record structural changes from ˗20°C to 40°C for a mesophilic enzyme in complex with inhibitors mimicking substrate-, intermediate-, and product-bound states, representative of major complexes on the reaction coordinate. Inhibitors, substrates and active site loops increasingly populated catalytically competent conformations as temperature increased. These changes occurred even in temperature ranges where kinetic measurements showed roughly linear Arrhenius/Eyring behavior, where parameters characterizing the system are assumed to be temperature independent. Simple analysis shows that linear Arrhenius/Eyring behavior can still be observed when the underlying activation energy/enthalpy values vary with temperature. Our results indicate a critical role for temperature dependent atomic-resolution structural data in interpreting temperature dependent kinetic data from enzymatic systems.","PeriodicalId":22168,"journal":{"name":"Structure","volume":"56 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-EM structure of the human monocarboxylate transporter 10

IF 5.7 2区生物学

Structure Pub Date : 2025-03-19 DOI: 10.1016/j.str.2025.02.012

Viktoria Bågenholm, Karl Patric Nordlin, Andrea Pasquadibisceglie, Andrey Belinskiy, Caroline Marcher Holm, Hajira Ahmed Hotiana, Kamil Gotfryd, Lucie Delemotte, Hussam Hassan Nour-Eldin, Per Amstrup Pedersen, Pontus Gourdon

{"title":"Cryo-EM structure of the human monocarboxylate transporter 10","authors":"Viktoria Bågenholm, Karl Patric Nordlin, Andrea Pasquadibisceglie, Andrey Belinskiy, Caroline Marcher Holm, Hajira Ahmed Hotiana, Kamil Gotfryd, Lucie Delemotte, Hussam Hassan Nour-Eldin, Per Amstrup Pedersen, Pontus Gourdon","doi":"10.1016/j.str.2025.02.012","DOIUrl":"https://doi.org/10.1016/j.str.2025.02.012","url":null,"abstract":"The monocarboxylate transporter (MCT) membrane protein family has 14 human members that perform key cellular functions, such as regulating metabolism. MCT8 and MCT10 have unique cargo specificity, transporting thyroid hormone and, in the case of MCT10, aromatic amino acids. Dysfunctional MCT8 causes the severe Allan-Herndon-Dudley syndrome, yet the (patho)physiology and function of MCT8 and MCT10 are not clearly understood, especially at a structural level. We present the cryoelectron microscopy (cryo-EM) structure of MCT10, displaying the classical major facilitator superfamily fold, caught in an inward-open configuration. Together with cargo docking models, the outward-open MCT10 AlphaFold model and validating functional analysis, cargo specificity and transport principles are proposed. These findings significantly enhance our understanding of the structure and function of MCTs, information that also may be valuable for the development of novel treatments against MCT-related disorders to address global challenges such as diabetes, obesity, and cancer.","PeriodicalId":22168,"journal":{"name":"Structure","volume":"61 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0