Molecular Nutrition & Food Research最新文献

{"title":"Rutin Enhances Intestinal Barrier Function and Protects Against X‐Ray Radiation‐Induced Barrier Dysfunction in Human Epithelial Caco‐2 Cells","authors":"Ha‐Young Park, Jin‐Hee Yu, Soo‐Bin Shin, Young‐Min Kim","doi":"10.1002/mnfr.70235","DOIUrl":"https://doi.org/10.1002/mnfr.70235","url":null,"abstract":"The small intestinal epithelium plays a critical role in nutrient absorption but is highly sensitive to radiation‐induced injury. Despite this vulnerability, therapeutic strategies to prevent or restore radiation‐induced intestinal barrier dysfunction remain limited. Polyphenols are known to protect the intestinal barrier by mitigating and repairing damage. Rutin, a quercetin‐3‐O‐rhamnosylglucoside with antioxidant, anticancer, antidiabetic, and antihypertensive activities, may help preserve intestinal epithelial integrity under radiation‐induced stress. This study investigated whether rutin could enhance intestinal barrier integrity and protect against X‐ray irradiation‐induced impairment in human epithelial Caco‐2 cell monolayers. Rutin pretreatment improved cell viability and barrier function, as shown by elevated transepithelial electrical resistance (TEER) and lowered fluorescein permeability. Additionally, rutin upregulated the mRNA and protein expression levels of key tight junction (TJ) markers, including ZO‐2, occludin, and claudin‐3. Notably, rutin pretreatment significantly attenuated X‐ray‐induced barrier dysfunction by restoring TEER, reducing paracellular permeability, and preventing the downregulation of TJ‐related genes and proteins. Rutin enhances intestinal barrier function and mitigates X‐ray‐induced barrier disruption by preventing the downregulation of ZO‐2, occludin, and claudin‐3 at both the mRNA and protein levels, thereby reinforcing epithelial integrity. Rutin may serve as a natural radioprotective agent, preserving gut barrier function during radiation exposure.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"52 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L‐Theanine Alleviates High‐Fat Diet‐Induced Obesity by Enhancing Hepatic Oxidative Phosphorylation and Remodeling Gut Microbiota to Augment Butyrate‐Producing","authors":"Lu Li, Jiayi Le, Yuele Lu, Yun Zhao, Yuanxiang Jin","doi":"10.1002/mnfr.70242","DOIUrl":"https://doi.org/10.1002/mnfr.70242","url":null,"abstract":"Obesity represents a significant global health challenge, necessitating innovative therapeutic approaches. L‐theanine, a bioactive component in tea, possesses diverse biological activities. This study investigates the anti‐obesity mechanisms of L‐theanine, focusing on its effects on lipid metabolism, energy expenditure, and gut microbiota using both in vitro and in vivo models. In vitro, L‐theanine (2 mM) reduced hepatocyte lipid content by downregulating lipogenic genes. In vivo, 12‐week supplementation with L‐theanine (30 and 100 mg/kg/day) dose‐dependently decreased HFD‐induced body weight gain, adipose tissue mass, and serum triglycerides (TG) without altering food intake. Mechanistically, L‐theanine disturbed the transcription of some key genes closely related to thermogenesis, lipid oxidation, and glycolipid metabolism, with hepatic transcriptomics confirming the enrichment of oxidative phosphorylation and thermogenic pathways. Critically, L‐theanine also regulates gut microbiota, specifically increasing the abundance of <jats:italic>Blautia</jats:italic> and enhancing short‐chain fatty acid biosynthesis. Collectively, these findings indicate that L‐theanine shows promise as a multi‐target therapeutic agent for obesity, regulating lipid metabolism, enhancing energy expenditure, and maintaining gut microbiota homeostasis. However, its molecular targets, mechanisms, and human applicability still remain unclear.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"26 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Effect of Krill Oil on Sortilin in Liver and Adipose Tissue in Obese Rats","authors":"Mehtap Atak, Ayse Beyza Sahin, Eda Yilmaz Kutlu, Hulya Kilic, Huseyin Avni Uydu, Hatice Sevim Nalkiran, Ertugrul Yigit","doi":"10.1002/mnfr.70233","DOIUrl":"https://doi.org/10.1002/mnfr.70233","url":null,"abstract":"<jats:label/>Sortilin regulates lipid metabolism, adipogenesis, and inflammation in obesity. While the anti‐inflammatory and lipid‐modulating effects of krill oil (KO) on obesity are well known, its effects on sortilin remain insufficiently investigated. This study investigates the potential effects of KO supplementation on sortilin and pro‐inflammatory cytokine levels in rats fed a high‐fat diet (HFD).<jats:label/>Twenty‐four male Sprague Dawley rats were randomly assigned to three groups: standard diet (SD), HFD, and HFD + KO (600 mg/kg/day KO via oral gavage for 12 weeks). Body weights were monitored weekly. Serum total cholesterol (TC), triglyceride, glucose, insulin, and HOMA‐IR were measured. Sortilin, TNF‐α, IL‐1β, and IL‐6 levels were assessed in liver and retroperitoneal (RP) adipose tissues. The HFD group showed significant increases in weight, TC, glucose, and HOMA‐IR, with decreased hepatic and increased RP adipose sortilin and TNF‐α levels. KO reduced serum TC, increased hepatic sortilin and TNF‐α, and decreased their levels in RP adipose tissue. These findings indicate that KO exerts a protective effect against HFD‐induced obesity and inflammation by modulating lipid metabolism, reducing TNF‐α and sortilin levels. Therefore, KO may serve as a promising dietary intervention for mitigating obesity‐related metabolic and inflammatory complications.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"26 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial Properties of Enterococcus faecium Strains Isolated From Bats’ Fecal Samples","authors":"Tatiana Alexandrovna Lipilkina, Marcos Vinício Alves, Kayque Ordonho Carneiro, Katia Leani, Igor V. Popov, Ilia V. Popov, Michael L. Chikindas, Alexey M. Ermakov, Svetoslav Dimitrov Todorov","doi":"10.1002/mnfr.70241","DOIUrl":"https://doi.org/10.1002/mnfr.70241","url":null,"abstract":"The principal objective of current study was to isolate and characterize <jats:italic>Enterococcus faecium</jats:italic> (<jats:italic>E. faecium</jats:italic>) strains from the fecal samples of <jats:italic>Nyctalus noctula</jats:italic> bats and to evaluate their probiotic potential and antimicrobial properties. Fecal samples were collected from bats in a rehabilitation center, and bacterial isolates were screened for antimicrobial activity against <jats:italic>Listeria monocytogenes</jats:italic>. Two bacteriocin‐producing strains, <jats:italic>E. faecium</jats:italic> ST01TL and ST76TL, were selected based on rep‐PCR fingerprinting and 16S rRNA sequencing. The proteinaceous nature of their antimicrobial substances was confirmed through protease treatment. These bacteriocins remained active under various pH, temperature, and chemical conditions. Both strains demonstrate high hydrophobicity, enzymatic activity with potential beneficial properties, and absence of hemolytic or mucin degradation activity. Molecular analyses revealed that studied strains carried genes for bacteriocin and GABA synthesis. However, some limited virulence traits or hazardous antibiotic resistance profiles were detected for both strains, a fact that merits additional attention and deeper investigations. Moreover, <jats:italic>E. faecium</jats:italic> ST01TL and ST76TL from bat feces possess general desirable probiotic traits and stable antimicrobial activity, suggesting their potential use in food safety and gut health improvement. These findings highlight bats as a novel source of probiotic strains with nutritional relevance. Further, in vivo studies, deeper safety assessments based on whole genome sequence analysis, and functional food applications are warranted to explore their full health‐promoting potential.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"26 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mango by‐Product‐Based High‐Fiber Confectionery Attenuates Liver Steatosis and Alters Gut Microbiota in High‐Fat Diet‐Fed Rats","authors":"Yuritzi Barbosa, Marcela Gaytán‐Martínez, Ericka A. de los Ríos‐Arellano, Rocio A. Chavez‐Santoscoy, Silvia Hinojosa‐Alvarez, Marilena Antunes‐Ricardo, Ivan Luzardo‐Ocampo, Aurea K. Ramírez‐Jiménez","doi":"10.1002/mnfr.70224","DOIUrl":"https://doi.org/10.1002/mnfr.70224","url":null,"abstract":"The incorporation of fruit by‐products into functional foods represents a promising strategy to address obesity. This study evaluated the nutritional potential of a novel extruded mango peel and bagasse‐based confectionery (MC) on obesity‐related parameters, gut microbiota composition, and liver/adipose tissue health. Twenty‐four male Wistar rats were assigned to four groups (<jats:italic>n</jats:italic> = 6): standard diet (STD), STD + MC (MC‐STD), high‐fat diet (HFD), and HFD + MC (MC‐HFD), over 11 weeks. MC supplementation increased fiber intake. Histologically, MC‐HFD rats showed significantly reduced liver fat accumulation and adipocyte hypertrophy compared to HFD controls. Gut microbiota analysis revealed that MC supplementation decreased obesogenic species such as Ruminococcus gnavus, Ruminococcus torques, Coprococcus catus, Coprococcus comes, and Clostridium hylemonae, while enhancing bacterial diversity. SCFAs such as isobutyric and acetic acids correlated positively with fiber intake and negatively with liver fat area and LDL levels. These findings highlight the nutritional relevance of using fruit processing by‐products in functional confections aimed at improving gutliver axis health and mitigating obesity risk in early life stages.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"52 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water‐Soluble Pu‐Erh Tea Extracts at Reasonable Oral Doses Down‐Regulate the Expression of Genes Involved in Fat Synthesis and Prevent Weight Gain in High‐Fat Diet‐Fed Mice","authors":"Yuhi Yamada, Yuki Shimba, Sayuri Shibayama, Chikako Maeda, Tsutomu Tahira, Kokoro Matsushita, Miku Ohta, Asuka Yamato, Fuzuki Nakamura, Yuichiro Obana, Chihiro Kubota, Kyoka Nakayama, Chihiro Honda, Shigenori Kumazawa, Toshio Hosaka","doi":"10.1002/mnfr.70228","DOIUrl":"https://doi.org/10.1002/mnfr.70228","url":null,"abstract":"Pu‐erh tea attenuates obesity and lipid metabolism by inhibiting pancreatic lipase, but prior studies used doses much higher than typical human consumption, which raised concerns about renal toxicity. This study aimed to investigate the effects of human drinkable doses of Pu‐erh tea extract (PTE) on obesity and lipid metabolism. High‐fat diet (HFD)‐fed male C57BL/6J mice were orally administered distilled water (HFD group), 107.5 mg/kg PTE (High group, human equivalent: 1200 mL), or half of the High group's dose (Low group, human equivalent: 600 mL) per day. Hepatic and visceral fat accumulation, and body weight normalized by caloric consumption were reduced in the High group compared to the HFD group. Additionally, PTE decreased plasma triglyceride (TG) and the gene expression of fat synthesis genes in the liver, concomitant with a reduction in histologically observed hepatic fat deposits. Furthermore, mRNA expression levels related to TG synthesis in adipocytes, decreased in response to PTE treatment. PTE administration, at a human drinkable dose, inhibited fat accumulation. This might be related to the decreased expressions of these genes in adipocytes or the liver involved in these functions, which would presumably contribute to the prevention of weight gain.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"26 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the Techno‐Functional and Biosafety Potential of a Novel Yeast Meyerozyma Guilliermondii YB1 as a Fermented Functional Food Starter: A Future Vision for Probiotics","authors":"Rajni Goyal, Richa Arora, Gurvinder Singh Kocher","doi":"10.1002/mnfr.70226","DOIUrl":"https://doi.org/10.1002/mnfr.70226","url":null,"abstract":"The present study investigates the techno‐functional and biosafety potential of <jats:italic>Meyerozyma guilliermondii</jats:italic> YB1 (OQ832654) as a novel starter culture for functional foods. <jats:italic>M. guilliermondii</jats:italic> YB1 exhibited xylanase and phytase activity, with an activity index (AI) of 0.66 and 0.21, respectively. Antimicrobial assays demonstrated inhibition against <jats:italic>Escherichia coli</jats:italic> MTCC 3222, <jats:italic>Salmonella typhimurium</jats:italic> MTCC 3224, and <jats:italic>Aspergillus</jats:italic> sp. CTS1, confirming its potential in food preservation. Furthermore, the yeast efficiently fermented a broad range of carbohydrates required for food fermentations. Auto‐aggregation increased from 25% at 2 h to 96.04% at 72 h, while hydrophobicity ranged from 38.54% to 66.16%, indicating strong gut adhesion properties. Growth at 37°C followed a normal sigmoidal curve, with optimal proliferation up to 48 h. Bile salt tolerance (0.3%) and acid resistance (pH 2.5–3.5) further confirmed its survival under gastrointestinal conditions. The non‐pathogenicity of <jats:italic>M. guilliermondii</jats:italic> YB1 was confirmed through antifungal susceptibility testing, absence of gelatinase, DNase activity, and a negative Congo red binding test, thereby supporting its use as a reliable functional food starter. Collectively, these attributes establish <jats:italic>M. guilliermondii</jats:italic> YB1 as a promising candidate for functional food applications, offering improved digestibility, food safety, and health benefits.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"13 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic Lactiplantibacillus plantarum Probio87 Improves Gut Microbial Profiles in HPV‐Positive Women: A Randomized, Placebo‐Controlled and Double‐Blind Study","authors":"Pei Xu, Mageswaran Uma Mageswary, Azka Ainun Nisaa, Sarah Binti Samsudin, Nurul Izza Binti Mohamed Rusdi, Abigail Rembui Anak Jerip, Chern‐Ein Oon, Salina Binti Sany, Cheng‐Siang Tan, Zhen‐Hua Zhu, Min‐Tze Liong","doi":"10.1002/mnfr.70247","DOIUrl":"https://doi.org/10.1002/mnfr.70247","url":null,"abstract":"HPV infection is a major global health concern, and recent evidence suggests that the gut microbiota may play a critical role in HPV progression and clearance. This study aimed to evaluate the modulatory effects of the probiotic strain <jats:italic>Lactiplantibacillus plantarum</jats:italic> Probio87 on the gut bacterial composition of HPV‐positive women. A 12‐week randomized, placebo‐controlled trial was conducted in accordance with the Declaration of Helsinki, with ethical approval from the Medical Research and Ethics Committee, Ministry of Health Malaysia (NMRR‐21‐1819‐61300), and registration at ClinicalTrials.gov (NCT05316064). Probiotic supplementation with <jats:italic>L. plantarum</jats:italic> Probio87 significantly modulated the gut microbiota. Specifically, reductions were observed in the relative abundance of potentially pathogenic bacteria, including <jats:italic>Gardnerella vaginalis</jats:italic> (<jats:italic>p</jats:italic> = 0.055), <jats:italic>Fannyhessea vaginae</jats:italic> (<jats:italic>p</jats:italic> = 0.039), and <jats:italic>Bilophila</jats:italic> species (<jats:italic>p</jats:italic> = 0.008). Conversely, levels of beneficial microbes such as <jats:italic>L. plantarum</jats:italic> (<jats:italic>p</jats:italic> = 0.001), <jats:italic>Anaerotignum lactatifermentans</jats:italic> (<jats:italic>p</jats:italic> = 0.039), <jats:italic>Blautia faecis</jats:italic> (<jats:italic>p</jats:italic> = 0.061), and <jats:italic>Roseburia hominis</jats:italic> A2‐183 (<jats:italic>p</jats:italic> = 0.052) were elevated. These findings suggest that <jats:italic>L. plantarum</jats:italic> Probio87 promotes gut microbial balance by reducing dysbiosis‐associated bacteria and enhancing beneficial populations. This modulation may contribute to improved vaginal and overall health in women with HPV infection.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"30 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High‐Fat Diet–Wheat Gluten Interactions in HLA‐DQ8 Transgenic Mice","authors":"Yuri Haneishi, Lucia Treppiccione, Vera Rotondi Aufiero, Francesco Maurano, Saya Watanabe, Diomira Luongo, Giuseppe Mazzarella, Junki Miyamoto, Mauro Rossi","doi":"10.1002/mnfr.70221","DOIUrl":"https://doi.org/10.1002/mnfr.70221","url":null,"abstract":"Unbalanced lipid metabolism contributes to inflammation in several conditions. Gluten, that triggers celiac disease, may also play a role in diseases associated with a high‐fat diet (HFD). Our aim was to investigate the interplay between gluten and HFD in HLA‐DQ8 (DQ8) transgenic mice, a model of gluten sensitivity. DQ8 mice were fed the gluten‐free diet, the HFD, or the HFD containing 8% gluten (HFD + G) for 12 or 23 weeks. Clinical parameters, liver and intestinal histology, immune parameters were assessed. Twelve weeks of HFD increased body and white adipose tissue weight and reduced glucose tolerance in male DQ8 mice. Gluten increased hyperinsulinemia and lipid accumulation in liver; interferon gamma (IFN‐γ) and interleukin‐10 (IL‐10) transcripts were higher in liver of HFD + G‐fed mice. IL‐1β, tumor necrosis factor‐α (TNF‐α), IFN‐γ, and IL‐10 mRNAs increased in the small intestine of HFD + G‐fed mice. However, gliadin‐specific immunity and alterations of the intestinal architecture were not induced. Feeding HFD + G for 23 weeks attenuated the gluten effect. A synergic effect between gluten and HFD after 12 weeks was found, which was instrumental to alter specific metabolic and immune functions in DQ8 mice. These findings provide new insights useful to dissect the association between obesity and gluten sensitivity.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"28 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144901662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Genomics and Probiotic Traits of Lactiplantibacillus plantarum MB685 from Fermented Broccoli: Gut Health and Metabolic Insights","authors":"Hina Irfan, Ifra Ghori, Wajya Ajmal, Bushra Bibi, Shakira Ghazanfar, Mohd Adnan, Adnan Ahmed Ansari","doi":"10.1002/mnfr.70209","DOIUrl":"https://doi.org/10.1002/mnfr.70209","url":null,"abstract":"The current study aimed to isolate and characterize probiotic bacteria with potential health benefits from fermented broccoli. Sixteen bacterial strains were isolated, and two strains (MB685 and MB686) were selected based on their probiotic potential. The isolated strains showed tolerance to pH and temperature variations, and survived well in bile salt concentrations of 0.3% (68%–76%) and 0.5% (61%–71%). MB685 was resistant to gentamicin, ofloxacin, and chloramphenicol, and showed antibacterial activity. Both strains showed significant auto‐aggregation (37%–66 % and 53%–43%), as well as cell surface hydrophobicity (56%–62%). MB685 inhibited pancreatic α‐amylase by 41.3%, suggesting potential anti‐diabetic effects. The 16S rRNA sequencing identified both strains as <jats:italic>Lactiplantibacillus plantarum</jats:italic>. Whole‐genome sequencing of MB685 revealed a genome size of 3.38 Mb with 3181 coding sequences, including plantaricin production genes (PlnA and PlnN) and carbohydrate‐active enzymes. No virulence factors and antibiotic resistance genes were observed, confirming their safety. <jats:italic>L. plantarum</jats:italic> MB685 is safe, effective, and has the potential for use as a starter culture and probiotic for gut health benefits.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"27 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144898816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}