Plant Pathology Journal最新文献_第2页

Mycological and Genomic Characterization of Fusarium vorosii, a Potentially Pathogenic Fungus, Isolated from Field Crops and Weeds in Korea. 从韩国大田作物和杂草中分离的一种潜在致病性真菌镰刀菌的真菌学和基因组学特征。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.08.2024.0121

Eunji Jeong, Jae Yun Lim, Jeong-Ah Seo

{"title":"Mycological and Genomic Characterization of Fusarium vorosii, a Potentially Pathogenic Fungus, Isolated from Field Crops and Weeds in Korea.","authors":"Eunji Jeong, Jae Yun Lim, Jeong-Ah Seo","doi":"10.5423/PPJ.OA.08.2024.0121","DOIUrl":"10.5423/PPJ.OA.08.2024.0121","url":null,"abstract":"Fusarium vorosii (Fv) is one of the least studied species of the Fusarium graminearum species complex, a major plant pathogen causing Fusarium head blight (FHB) in a variety of crops. In this study, we isolated 12 strains of Fv from cereal samples with FHB symptoms and gramineous weeds. Trichothecene genotyping of Fv strains showed that 10 strains were nivalenol (NIV) type and 2 strains were 15-acetyldeoxynivalenol (15ADON) type. Fv strains have similar mycological characteristics to Fusarium asiaticum, a major FHB pathogen of rice in Asia, however, asexual sporulation was at least 100 to 1,000 times higher in Fv. In comparison of pathogenicity, the Fv-15ADON type was more pathogenic than the NIV type in both rice and wheat, and had a similar level of pathogenicity as the F. asiaticum-NIV type. Among the 12 Fv strains, two representative ones, Fv-NIV type RN1 and Fv-15ADON type W15A1, were selected and their whole genomes were sequenced and analyzed. Complete genome sequences of two Fv strains, RN1 and W15A1, were assembled at the chromosome level with high quality compared to known Fv genomes. The genome data of the two Fv strains were compared with the reference strains already known. As a result of comparative genome analysis, it was found that they are phylogenetically related according to the trichothecene biosynthetic gene cluster, that is, toxin chemotype. Through this study, we provided important information about Fv species that can be potential pathogens in domestic crops about biological and genomic characteristics.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"656-670"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of TaqMan-Based Real-Time qPCR Method for Accurate Detection and Quantification of Citrus Psorosis Virus and Cytoplasmic-Type Citrus Leprosis Virus in Saplings. 基于taqman的柑橘病病毒和细胞质型柑橘麻风病毒实时荧光定量检测方法的建立

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.09.2024.0134

Minhue Jung, Na Hee Kim, Seung Hyeon Oh, Kook-Hyung Kim

{"title":"Development of TaqMan-Based Real-Time qPCR Method for Accurate Detection and Quantification of Citrus Psorosis Virus and Cytoplasmic-Type Citrus Leprosis Virus in Saplings.","authors":"Minhue Jung, Na Hee Kim, Seung Hyeon Oh, Kook-Hyung Kim","doi":"10.5423/PPJ.OA.09.2024.0134","DOIUrl":"10.5423/PPJ.OA.09.2024.0134","url":null,"abstract":"In 2022, citrus fruits were the second most widely produced fruit globally, highlighting their significant role in the fruit industry. However, due to their clonal propagation, these fruits are highly susceptible to viral infections, posing challenges for growers. In response to the booming nursery market, the Korean plant quarantine station reported over 80 million sapling stocks, with 15% being discarded after rigorous inspection due to contamination or disease. As the global nursery trade continues to expand, there is an urgent need for a fast and accurate diagnostic tool to ensure the health of plant stocks. In this study, we developed a TaqMan-based real-time reverse transcription-quantitative PCR assay specifically designed to detect two critical citrus viruses: citrus psorosis virus and citrus leprosis virus C. Our assay demonstrated the capability to detect virus sequences with as few as 30 copies, maintaining high PCR efficiency with RNA extracted from both twig and leaf tissues. Additionally, we incorporated an artificial sequence into the positive controls, which effectively served as a marker for detecting potential sample contamination. This comprehensive diagnostic system promises to enhance plant quarantine measures and phytosanitation practices, providing a reliable and efficient solution to safeguard citrus crops from viral threats.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"625-632"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Tomato Seed Endophytic Bacteria as Growth Promoters and Potential Biocontrol Agents. 番茄种子内生细菌作为生长促进剂和潜在生物防治剂的特性研究。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.09.2024.0142

Mehwish Roy, Byeonghyeok Kang, Seongeun Yang, Heeyun Choi, Kihyuck Choi

{"title":"Characterization of Tomato Seed Endophytic Bacteria as Growth Promoters and Potential Biocontrol Agents.","authors":"Mehwish Roy, Byeonghyeok Kang, Seongeun Yang, Heeyun Choi, Kihyuck Choi","doi":"10.5423/PPJ.OA.09.2024.0142","DOIUrl":"10.5423/PPJ.OA.09.2024.0142","url":null,"abstract":"Endophytic bacteria residing within plant seeds are increasingly recognized for their potential to enhance plant growth and provide biocontrol against pathogens. Despite this, seed-borne endophytes remain underexplored in many crops, including tomato. In this study, we isolated and characterized bacterial endophytes from tomato seeds and evaluated their plant growth-promoting traits and antifungal activities. The taxonomic analysis of the Hawaii 7996 tomato seed endophyte collection revealed a diverse community, predominantly from the phylum Bacillota, with Paenibacillaceae and Bacillaceae as the most abundant families. Among the 35 unique strains identified, 19 produced indole-3-acetic acid, four exhibited siderophore production, and 12 could solubilize phosphate. These traits contribute to growth promotion and disease suppression in plants. In the plant growth promotion assay, several bacterial strains, notably Streptomyces olivaceus (BHM1), Streptomyces variegatus (BHM3), Bacillus stercoris (BHR2), and Moraxella osloensis (YHT4-1), demonstrated significant potential for tomato cultivation by positively affecting fresh weight, stem length, and root length. These strains consistently promoted growth across all three parameters evaluated in this study. Furthermore, several strains exhibited strong antifungal activity against major tomato pathogens, including Fusarium oxysporum race 1 and 2, and Botrytis cinerea. Notably, Bacillus subtilis (BHN1), Bacillus stercoris (BHR2), and Paenibacillus peoriae (YHR2-1) showed broad-spectrum antifungal efficacy. Our findings highlight the potential of seed-associated endophytic bacteria as growth promoters and biological control agents, offering promising avenues for sustainable agricultural practices.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"578-592"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capsicum annuum NAC4 (CaNAC4) Is a Transcription Factor with Roles in Biotic and Abiotic Stresses. 辣椒 NAC4（CaNAC4）是一种在生物和非生物压力中发挥作用的转录因子。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.07.2024.0104

Guogeng Jia, Khaing Shwe Zin Thinn, Sun Ha Kim, Jiyoung Min, Sang-Keun Oh

{"title":"Capsicum annuum NAC4 (CaNAC4) Is a Transcription Factor with Roles in Biotic and Abiotic Stresses.","authors":"Guogeng Jia, Khaing Shwe Zin Thinn, Sun Ha Kim, Jiyoung Min, Sang-Keun Oh","doi":"10.5423/PPJ.OA.07.2024.0104","DOIUrl":"https://doi.org/10.5423/PPJ.OA.07.2024.0104","url":null,"abstract":"Transcription factors (TFs) regulate gene expression by binding to DNA. The NAC gene family in plants consists of crucial TFs that influence plant development and stress responses. The whole genome of Capsicum annuum shows over 100 NAC genes (CaNAC). Functional characteristics of the most CaNAC TFs are unknown. In this study, we identified CaNAC4, a novel NAC TF in C. annuum. CaNAC4 expression increased after inoculation with the pathogens, Xanthomonas axonopodis pv. vesicatoria race 3 and X. axonopodis pv. glycines 8ra, and following treatment with the plant hormones, salicylic acid and abscisic acid. We investigated the functional characteristics of the CaNAC4 gene and its roles in salt tolerance and anti-pathogen defense in transgenic Nicotiana benthamiana. For salt stress analysis, the leaf discs of wild-type and CaNAC4-transgenic N. benthamiana plants were exposed to different concentrations of sodium chloride. Chlorophyll loss was more severe in salt stress-treated wild-type plants than in CaNAC4-transgenic plants. To analyze the role of CaNAC4 in anti-pathogen defense, a spore suspension of Botrytis cinerea was used to infect the leaves. The disease caused by B. cinerea gradually increased in severity, and the symptoms were clearer in the CaNAC4-transgenic lines. We also investigated hypersensitive response (HR) in CaNAC4-transgenic plants. The results showed a stronger HR in wild-type plants after infiltration with the apoptosis regulator, BAX. In conclusion, our results suggest that CaNAC4 may enhance salt tolerance and act as a negative regulator of biotic stress in plants.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"512-524"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ralstonia solanacearum Infection Drives the Assembly and Functional Adaptation of Potato Rhizosphere Microbial Communities. Ralstonia solanacearum 感染驱动马铃薯根瘤微生物群落的组装和功能适应。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.06.2024.0086

Zhang Qing, Yang Jida, Fu Chengxiu, Yang Yanli, Liu Xia, Deng Sihe

{"title":"Ralstonia solanacearum Infection Drives the Assembly and Functional Adaptation of Potato Rhizosphere Microbial Communities.","authors":"Zhang Qing, Yang Jida, Fu Chengxiu, Yang Yanli, Liu Xia, Deng Sihe","doi":"10.5423/PPJ.OA.06.2024.0086","DOIUrl":"https://doi.org/10.5423/PPJ.OA.06.2024.0086","url":null,"abstract":"Bacterial wilt caused by Ralstonia solanacearum is a destructive disease that affects potato production, leading to severe yield losses. Currently, little is known about the changes in the assembly and functional adaptation of potato rhizosphere microbial communities during different stages of R. solanacearum infection. In this study, using amplicon and metagenomic sequencing approaches, we analyzed the changes in the composition and functions of bacterial and fungal communities in the potato rhizosphere across four stages of R. solanacearum infection. The results showed that R. solanacearum infection led to significant changes in the composition and functions of bacterial and fungal communities in the potato rhizosphere, with various microbial properties (including α,β-diversity, species composition, and community ecological functions) all being driven by R. solanacearum infection. The relative abundance of some beneficial microorganisms in the potato rhizosphere, including Firmicutes, Bacillus, Pseudomonas, and Mortierella, decreased as the duration of infection increased. Moreover, the related microbial communities played a significant role in basic metabolism and signal transduction; however, the functions involved in soil C, N, and P transformation weakened. This study provides new insights into the dynamic changes in the composition and functions of potato rhizosphere microbial communities at different stages of R. solanacearum infection to adapt to the growth promotion or disease suppression strategies of host plants, which may provide guidance for formulating future strategies to regulate microbial communities for the integrated control of soil-borne plant diseases.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"498-511"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Hibiscus Chlorotic Ringspot Virus-Derived vsiRNAs from Infected Hibiscus rosa-sinensis in China. 从中国受感染的木槿中鉴定木槿萎黄环斑病毒产生的 vsiRNA。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.06.2024.0090

Han-Hong Lan, Luan-Mei Lu

{"title":"Characterization of Hibiscus Chlorotic Ringspot Virus-Derived vsiRNAs from Infected Hibiscus rosa-sinensis in China.","authors":"Han-Hong Lan, Luan-Mei Lu","doi":"10.5423/PPJ.OA.06.2024.0090","DOIUrl":"https://doi.org/10.5423/PPJ.OA.06.2024.0090","url":null,"abstract":"Lots of progress have been made about pathogen system of Hibiscus rosa-sinensis and hibiscus chlorotic ringspot virus (HCRSV), however, interactions between H. rosa-sinensis and HCRSV remain largely unknown. Hereon, firstly, HCRSV infection in H. rosa-sinensis from Zhangzhou city of China was confirmed by traditional electron microscopy, modern reverse transcription polymerase chain reaction and RNA-seq methods. Secondly, sequence feature analysis showed the full-length sequence of HCRSV-ZZ was 3,909 nucleotides (nt) in length and had a similar genomic structure with other carmovirus. It contains a 5' untranslated region (UTR), followed by seven open reading frames encoding for P28, P23, P81, P8, P9, P38, and P25, and the last a 3-terminal UTR. Thirdly, HCRSV- ZZ-derived vsiRNAs were identified and characterized for the first time from disease H. rosa-sinensis through sRNA-seq to reveal interactions between pathogen ant plant host. It was shown that the majority of HCRSV-ZZ-derived vsiRNAs were 21 nt, 22 nt, and 20 nt, with 21 nt being most abundant. The 5'-terminal nucleotide of HCRSV-ZZ vsiRNAs preferred U and C. HCRSV-ZZ vsiRNAs derived predominantly (72%) from the viral genome positive-strand RNA. The distribution of HCRSV-ZZ vsiRNAs along the viral genome is generally even, with some hot spots and cold spots forming in local regions. These hot spots and cold spots could be corresponded to the regions of stem loop secondary structures forming in HCRSV-ZZ genome by nucleotide paring. Taken together, our findings certify HCRSV infection in H. rosa-sinensis and provide an insight into interaction between HCRSV and H. rosa-sinensis and contribute to the prevention and treatment of this virus.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"415-424"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-identification of Korean Isolates in the Colletotrichum dematium, C. magnum, C. orchidearum, and C. orbiculare Species Complexes. 重新鉴定韩国鹅膏蕈菌（Colletotrichum dematium）、鹅膏蕈菌（C. magnum）、兰花蕈菌（C. orchidearum）和鹅膏蕈菌（C. orbiculare）物种群中的分离株。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.05.2024.0081

Le Dinh Thao, Hyorim Choi, Donghun Kang, Anbazhagan Mageswari, Daseul Lee, Dong-Hyun Kim, In-Young Choi, Hyeon-Dong Shin, Seung-Beom Hong

{"title":"Re-identification of Korean Isolates in the Colletotrichum dematium, C. magnum, C. orchidearum, and C. orbiculare Species Complexes.","authors":"Le Dinh Thao, Hyorim Choi, Donghun Kang, Anbazhagan Mageswari, Daseul Lee, Dong-Hyun Kim, In-Young Choi, Hyeon-Dong Shin, Seung-Beom Hong","doi":"10.5423/PPJ.OA.05.2024.0081","DOIUrl":"https://doi.org/10.5423/PPJ.OA.05.2024.0081","url":null,"abstract":"A large number of species in the genus Colletotrichum have been reported as causal agents of anthracnose on crops and wild plants in Korea. Many Colletotrichum isolates from the country preserved in the Korean Agricultural Culture Collection (KACC) were previously identified based on host plants and morphological characteristics, and it may lead to species misidentification. Thus, accurate fungal species identification using multilocus sequence analyses is essential for understanding disease epidemiology and disease management strategies. In this study, combined DNA sequence analyses of internal transcribed spacer, gapdh, chs-1, his3, act, tub2, and gs were applied to re-identify 27 Colletotrichum isolates in KACC. The phylogenetic analyses showed that the isolates resulted in 11 known species, they belong to the C. dematium species complex (C. hemerocallidis, C. jinshuiense, and C. spinaciae), the C. magnum complex (C. kaifengense and C. cf. ovatense), the C. orchidearum complex (C. cattleyicola, C. plurivorum, C. reniforme, and C. sojae) and the C. orbiculare complex (C. malvarum and C. orbiculare). Of them, C. cattleyicola, C. hemerocallidis, C. kaifengense, and C. reniforme were unrecorded species in Korea. In the view of host-fungus combinations, 10 combinations are newly reported in the world and 12 are new reports in Korea, although their pathogenicity on the host was not confirmed.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"425-437"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cestrum tomentosum L.f. Extracts against Colletotrichum scovillei by Altering Cell Membrane Permeability and Inducing ROS Accumulation. Cestrum tomentosum L.f.提取物通过改变细胞膜渗透性和诱导ROS积累来对抗Colletotrichum scovillei。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.07.2024.0105

Guogeng Jia, Sun Ha Kim, Jiyoung Min, Nelson Villalobos Zamora, Silvia Soto Montero, Soo-Yong Kim, Sang-Keun Oh

{"title":"Cestrum tomentosum L.f. Extracts against Colletotrichum scovillei by Altering Cell Membrane Permeability and Inducing ROS Accumulation.","authors":"Guogeng Jia, Sun Ha Kim, Jiyoung Min, Nelson Villalobos Zamora, Silvia Soto Montero, Soo-Yong Kim, Sang-Keun Oh","doi":"10.5423/PPJ.OA.07.2024.0105","DOIUrl":"https://doi.org/10.5423/PPJ.OA.07.2024.0105","url":null,"abstract":"Chili pepper anthracnose, caused by Colletotrichum spp., is a significant biotic stress affecting chili fruits globally. While fungicide application is commonly used for disease management due to its efficiency and costeffectiveness, excessive use poses risks to human health and the environment. Botanical fungicides offer advantages such as rapid degradation and low toxicity to mammals, making them increasingly popular for sustainable plant disease control. This study investigated the antifungal properties of Cestrum tomentosum L.f. crude extracts (CTCE) against Colletotrichum scovillei. The results demonstrated that CTCE effectively inhibited conidia germination and germ tube elongation at 40 µg/ml concentrations. Moreover, CTCE exhibited strong antifungal activity against C. scovillei mycelial growth, with an EC50 value of 18.81 µg/ml. In vivo experiments confirmed the protective and curative effects of CTCE on chili pepper fruits infected with C. scovillei. XTT analysis showed that the CTCE could significantly inhibit the cell viability of C. scovillei. Mechanistic studies revealed that CTCE disrupted the plasma membrane integrity of C. scovillei and induced the accumulation of reactive oxygen species in hyphal cells. These findings highlight CTCE as a promising eco-friendly botanical fungicide for managing C. scovillei infections in chili peppers.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"475-485"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis MORC1 and MED9 Interact to Regulate Defense Gene Expression and Plant Fitness. 拟南芥 MORC1 和 MED9 相互作用，调控防御基因表达和植物健壮性

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.07.2024.0107

Ji Chul Nam, Padam Shekhar Bhatt, April Bonnard, Dinesh Pujara, Hong-Gu Kang

{"title":"Arabidopsis MORC1 and MED9 Interact to Regulate Defense Gene Expression and Plant Fitness.","authors":"Ji Chul Nam, Padam Shekhar Bhatt, April Bonnard, Dinesh Pujara, Hong-Gu Kang","doi":"10.5423/PPJ.OA.07.2024.0107","DOIUrl":"https://doi.org/10.5423/PPJ.OA.07.2024.0107","url":null,"abstract":"Arabidopsis MORC1 (Microrchidia) is required for multiple levels of immunity. We identified 14 MORC1-interacting proteins (MIPs) via yeast two-hybrid screening, eight of which have confirmed or putative nuclear-associated functions. While a few MIP mutants displayed altered bacterial resistance, MIP13 was unusual. The MIP13 mutant was susceptible to Pseudomonas syringae, but when combined with morc1/2, it regained wild-type resistance; notably, morc1/2 is susceptible to the same pathogen. MIP13 encodes MED9, a mediator complex component that interfaces with RNA polymerase II and transcription factors. Expression analysis of defense genes PR1, PR2, and PR5 in response to avirulent P. syringae revealed that morc1/2 med9 expressed these genes in a slow but sustained manner, unlike its lower-order mutants. This expression pattern may explain the restored resistance and suggests that the interplay of MORC1/2 and MED9 might be important in curbing defense responses to maintain fitness. Indeed, repeated challenges with avirulent P. syringae triggered significant growth inhibition in morc1/2 med9, indicating that MED9 and MORC1 may play an important role in balancing defense and growth. Furthermore, the in planta interaction of MED9 and MORC1 occurred 24 h, not 6 h, postinfection, suggesting that the interaction functions late in the defense signaling. Our study reveals a complex interplay between MORC1 and MED9 in maintaining an optimal balance between defense and growth in Arabidopsis.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"438-450"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis WRKY55 Transcription Factor Enhances Soft Rot Disease Resistance with ORA59. 拟南芥 WRKY55 转录因子通过 ORA59 增强软腐病抗性

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.08.2024.0126

Ji Eun Kang, Hyunsun Kim, Kyungyoung Song, Changhyun Choi, Yun Ju Kim, Duk-Ju Hwang, Eui-Hwan Chung

引用次数: 0