Biological Control and Growth-Promoting Activities of Burkholderia vietnamiensis FBCC-B8049 Isolated from Freshwater.

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Gil Han, Wonsu Cheon, Yunjeong Heo, Chang Soo Lee
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Abstract

Colletotrichum and Fusarium are globally important plant-pathogenic fungi that cause serious diseases in chili pepper and other crops, leading to substantial yield losses due to their broad host range, environmental persistence, and the limited effectiveness of chemical control, thereby highlighting the need for sustainable alternatives such as biological control. We investigate the biological control and plant growth-promoting potential of Burkholderia vietnamiensis FBCC-B8049, isolated from freshwater environments. The strain exhibited significant antifungal activity against Colletotrichum gloeosporioides, Colletotrichum acutatum, and Fusarium oxysporum in dual-culture assays, with stronger effects against Colletotrichum species. The inhibition was likely due to direct antagonism and volatile organic compounds (VOCs) produced by FBCC-B8049, which were particularly effective against Colletotrichum species. Additionally, FBCC-B8049 demonstrated plant growth-promoting activities including siderophore production for iron acqusition, phosphate solubilization for enhanced nutrient availability, and indole-3-acetic acid synthesis to promote root development. These combined activities enhance nutrient availability and promote seed germination and seedling growth in chili pepper. Ex vivo assays further revealed the effectiveness of FBCC-B8049 in suppressing anthracnose disease on pepper fruits through both direct application and bacterial VOCs emission. Phylogenetic analysis of 16S rRNA and recA gene sequences positioned FBCC-B8049 closely to Burkholderia vietnamiensis, a known plant growth-promoting rhizobacterium. These findings highlight FBCC-B8049 as a promising candidate for sustainable agricultural applications.

淡水分离越南伯克氏菌FBCC-B8049的生物防治及促生长活性研究
炭疽菌和镰刀菌是全球重要的植物病原真菌,可引起辣椒和其他作物的严重疾病,由于其广泛的寄主范围、环境持久性和化学控制的有效性有限,导致大量产量损失,因此强调需要可持续的替代方案,如生物控制。研究了从淡水环境中分离得到的越南伯克霍尔德菌FBCC-B8049的生物防治和植物促生潜力。该菌株对炭疽菌、尖锐炭疽菌和尖孢镰刀菌均表现出显著的抗真菌活性,其中对炭疽菌有较强的抑制作用。这种抑制作用可能与FBCC-B8049产生的挥发性有机化合物(VOCs)直接拮抗作用有关,其中挥发性有机化合物对炭疽菌尤为有效。此外,FBCC-B8049还显示出促进植物生长的活性,包括产生铁载体以获取铁,增加磷酸盐的增溶性以提高养分有效性,以及合成吲哚-3-乙酸以促进根系发育。这些共同作用提高了辣椒的养分利用率,促进了种子萌发和幼苗生长。体外实验进一步揭示了FBCC-B8049通过直接施用和细菌挥发性有机化合物(VOCs)排放抑制辣椒果实炭疽病的有效性。16S rRNA和recA基因序列的系统发育分析表明,FBCC-B8049与已知的植物促生长根瘤菌越南伯克霍尔德菌(Burkholderia vietnamiensis)接近。这些发现突出了fbc - b8049作为可持续农业应用的有前途的候选者。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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