Plant Pathology Journal最新文献

Functional Analysis of KPvRxLR27, a Novel Plasmopara viticola Effector from a Korean Isolate, and Its Role in Hypersensitive Response. 韩国葡萄浆原体效应蛋白KPvRxLR27的功能分析及其在过敏反应中的作用

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-16 DOI: 10.5423/PPJ.OA.09.2024.0141

Marc Semunyana, Rahel Dinsa Guta, Guogeng Jia, Soomin Lee, Inyong Jeong, Khaing Shwe Zin Thinn, DoYoon Lim, Siyeon Lim, Bomi Lee, HyunJu Kim, May Moe Oo, Sun Ha Kim, Jiyoung Min, Sang-Keun Oh

{"title":"Functional Analysis of KPvRxLR27, a Novel Plasmopara viticola Effector from a Korean Isolate, and Its Role in Hypersensitive Response.","authors":"Marc Semunyana, Rahel Dinsa Guta, Guogeng Jia, Soomin Lee, Inyong Jeong, Khaing Shwe Zin Thinn, DoYoon Lim, Siyeon Lim, Bomi Lee, HyunJu Kim, May Moe Oo, Sun Ha Kim, Jiyoung Min, Sang-Keun Oh","doi":"10.5423/PPJ.OA.09.2024.0141","DOIUrl":"https://doi.org/10.5423/PPJ.OA.09.2024.0141","url":null,"abstract":"Plasmopara viticola causes grape downy mildew, one of the most notorious diseases of cultivated grapes that damage vineyards worldwide. The pathogen secretes various effector molecules to infect and modulate the host biological processes. In this study, we aimed to evaluate the roles of KPvRxLR27, an arginine-any amino acid-leucine-arginine (RxLR) effector isolated from P. viticola JN-9 from Jeonju (South Korea) with respect to the reported Bcl-2-associated X and inverted formin1in inducing cell death in non-host Nicotiana benthamiana and resistant grape host cultivars via Agrobacterium-mediated transient transformation. We found that, KPvRxLR27 induced programmed cell death in N. benthamiana and rapid hypersensitive response in resistant grape cultivars. Agroinfiltration assay revealed that putative N-glycosylation at the N186 amino acid sequence and nuclear localization signal motifs at the C-terminus were critical for the effector's cell death-inducing activity of KPvRxLR27. Overexpression assay revealed that KPvRxLR27 was abundantly expressed in the plasma membrane and nuclear regions and activated the accumulation of reactive oxygen species in N. benthamiana. Moreover, KPvRxLR27 expression was significantly delayed in the resistant cultivar than in the susceptible cultivar. Our results suggest KPvRxLR27 as a potential avirulence gene recognized by the host receptors to activate the host immune response-associated genes, providing valuable insights to enhance the pathogen resistance of commercial cultivars.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protection Efficacy of Antibacterial Strains against Fire Blight Caused by Erwinia amylovora on Apple Blossom. 抑菌菌株对苹果花火枯病的保护效果研究。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.09.2024.0148

Sujeong Kim, Yong Ho Shin, Sonja Weißhaupt, Stefan Kunz, Yong Chull Jeun

{"title":"Protection Efficacy of Antibacterial Strains against Fire Blight Caused by Erwinia amylovora on Apple Blossom.","authors":"Sujeong Kim, Yong Ho Shin, Sonja Weißhaupt, Stefan Kunz, Yong Chull Jeun","doi":"10.5423/PPJ.OA.09.2024.0148","DOIUrl":"10.5423/PPJ.OA.09.2024.0148","url":null,"abstract":"Fire blight caused by Erwinia amylovora is one of the destructive diseases in the family of Rosaceae plants, including apple and pear, in the world. Since the first report in 2015, the number of infected farms and area steadily increased in Korea. In case of eradication failure against this disease, protection strategies using both chemicals and biocontrol agents should be established. In this study, to select an effective antibacterial agent against fire blight on apple trees, four bacterial strains isolated from Jeju Island were investigated. Among the bacterial strains, Bacillus circulans BRH433-2 showed bactericidal effects against E. amylovora Ea385 forming inhibition zone on an artificial medium. The other bacterial strains such as Pseudomonas fluorescens THJ609-3, Micrococcus luteus TRK2-2 and P. fluorescens TRH415-2 showed bacteriostatic activity preventing growth of E. amylovora Ea385 in shaken cultures as well as on detached apple blossoms inoculated with E. amylovora Ea385, as measured with quantitative PCR. Bio-tests on detached blossoms showed that the treatment with all bacterial strains caused strong suppression of bacterial ooze formation, indicating inhibition of disease incidence of fire blight, which was similar to blossoms treated with streptomycin sulfate. Therefore, it was suggested that these bacterial strains may be useful in organic apple orchards to control fire blight where chemical use is limited.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"633-640"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and Characterization of a Full-Length Infectious cDNA Clone of a Strain of Watermelon Mosaic Virus Isolated from Melon. 西瓜花叶病毒全长感染性cDNA克隆的构建与鉴定。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.07.2024.0103

Hae-Ryun Kwak, Su-Bin Hong, Jeong-Eun Kim, Hee-Seong Byun, Bong-Choon Lee, Hong-Soo Choi, Mikyeong Kim

{"title":"Construction and Characterization of a Full-Length Infectious cDNA Clone of a Strain of Watermelon Mosaic Virus Isolated from Melon.","authors":"Hae-Ryun Kwak, Su-Bin Hong, Jeong-Eun Kim, Hee-Seong Byun, Bong-Choon Lee, Hong-Soo Choi, Mikyeong Kim","doi":"10.5423/PPJ.OA.07.2024.0103","DOIUrl":"10.5423/PPJ.OA.07.2024.0103","url":null,"abstract":"Watermelon mosaic virus (WMV), a member of the genus Potyvirus, causes serious economic losses in cucurbit crops. For molecular biological studies of viruses, it is necessary to construct an infectious clone that can facilitate gene functional analysis. In this study, we constructed an infectious cDNA clone of WMV genomic RNA by Gibson assembly and evaluated its virulence and symptoms on a variety of host plants. A WMV isolate (WMV-IS-me2) collected from melon in Iksan, Jeonbuk Province, Korea in 2015 caused mosaic symptoms on leaves. Four overlapping PCR fragments of the full-length genome of this isolate and the pJL89 binary vector with overhanging ends of 40 bp were amplified by reverse transcription PCR and joined in a single isothermal reaction. The complete nucleotide sequence of the infectious cDNA clone of WMV was determined and the recombinant vector was transformed into Agrobacterium tumefaciens GV3101. Following agro-infiltration, an infectious clone (pWMV-M, KACC95145P) systemically induced mosaic symptoms on watermelon (Citrullus lanatus), zucchini (Cucurbita pepo), melon (Cucumis melo), and Nicotiana benthamiana plants. This infectious clone may be useful for screening cucurbit varieties resistant to WMV, as well as for studying viral gene function.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"615-624"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Comparison between Resistant and Susceptible Soybean Cultivars in Response to Inoculation of Phytophthora sojae. 抗感大豆品种对接种大豆疫霉的转录组比较

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.09.2024.0154

Sangrea Shim, In-Jeong Kang, Hee Jin You, Hangil Kim, Sungwoo Lee

{"title":"Transcriptome Comparison between Resistant and Susceptible Soybean Cultivars in Response to Inoculation of Phytophthora sojae.","authors":"Sangrea Shim, In-Jeong Kang, Hee Jin You, Hangil Kim, Sungwoo Lee","doi":"10.5423/PPJ.OA.09.2024.0154","DOIUrl":"10.5423/PPJ.OA.09.2024.0154","url":null,"abstract":"Phytophthora root and stem rot, caused by Phytophthora sojae, considerably reduces soybean yield worldwide. Our previous study identified two genomic regions on chromosome 18 (2.1-2.6 and 53.1-53.3 Mbp) that confer resistance to the P. sojae isolate 2457, through linkage analysis using progenies derived from the Daepung × Socheong2 population. These two regions contained 51 and 19 annotated genes, respectively. However, the specific gene responsible for resistance to P. sojae isolate 2457 has yet to be identified. In this study, we performed a comparative transcriptomic analysis of Socheong2 and Daepung, two Korean soybean varieties identified as resistant and susceptible to P. sojae isolate 2457, respectively. RNA sequencing was conducted on tissue samples collected at 0, 6, and 12 hours after inoculation (HAI), and significant differences in the expression of defense-related genes were observed across time points and between the two cultivars. Genes associated with the jasmonic acid, salicylic acid, ethylene, and systemic acquired resistance pathways were upregulated in both cultivars at 6 and 12 HAI compared to 0 HAI, with these biological processes were more strongly upregulated in Socheong2 compared to Daepung at 6 and 12 HAI. A comparison of differentially expressed genes (DEGs) and candidate genes within the previously identified QTL regions revealed an ortholog of the HS1 PRO-1 2 gene from Arabidopsis thaliana among the upregulated DEGs in Socheong2, particularly at 12 HAI compared to 0 HAI. This study will aid in targeted breeding efforts to develop soybean varieties with improved resistance to P. sojae.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"641-655"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Biocontrol Efficacy of Bacillus velezensis GYUN-1190 against Apple Bitter Rot. 香蕉芽孢杆菌gun -1190对苹果苦腐病的防治效果研究

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.05.2024.0076

Hyeonjin Park, Younmi Lee, Kotnala Balaraju, Jungyeon Kim, Yongho Jeon

{"title":"Characterization and Biocontrol Efficacy of Bacillus velezensis GYUN-1190 against Apple Bitter Rot.","authors":"Hyeonjin Park, Younmi Lee, Kotnala Balaraju, Jungyeon Kim, Yongho Jeon","doi":"10.5423/PPJ.OA.05.2024.0076","DOIUrl":"10.5423/PPJ.OA.05.2024.0076","url":null,"abstract":"The application of synthetic fungicides has resulted in environmental pollution and adverse effects on non-target species. To reduce the use of agrochemicals, crop disease management requires microbial biological control agents. Bacillus-related genera produce secondary metabolites to control fungal pathogens. Bacillus velezensis GYUN-1190, isolated from soil, showed antagonistic activity against Colletotrichum fructicola, the apple anthracnose pathogen. Volatile organic compounds and culture filtrate (CF) from GYUN-1190 inhibited C. fructicola growth in vitro, by 80.9% and 30.25%, respectively. The CF of GYUN-1190 inhibited pathogen spore germination more than cell suspensions at 10 8 cfu/ml. Furthermore, GYUN-1190 CF is effective in inhibiting C. fructicola mycelial growth in vitro, and it suppresses apple fruit bitter rot more effectively than GYUN-1190 cell suspensions and pyraclostrobin in planta. The mycelial growth of C. fructicola was completely inhibited 48 h after immersion into the CF, in compared with positive controls and GYUN-1190 cell suspensions. The genetic mechanism underlying the biocontrol features of GYUN-1190 was defined using its whole-genome sequence, which was closely compared to similar strains. It consisted of 4,240,653 bp with 45.9% GC content, with 4,142 coding sequences, 87 tRNA, and 28 rRNA genes. The genomic investigation found 14 putative secondary metabolite biosynthetic gene clusters. The investigation suggests that B. velezensis GYUN-1190 might be more effective than chemical fungicides and could address its potential as a biological control agent.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"681-695"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening Method to Identify Watermelon Cultivars Resistant to Acidovorax citrulli, the Cause of Bacterial Fruit Blotch. 西瓜抗细菌性果实斑病的筛选方法研究。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.08.2024.0122

Soo Min Lee, Gahyeon Kim, Hun Kim, Gyung Ja Choi

{"title":"Screening Method to Identify Watermelon Cultivars Resistant to Acidovorax citrulli, the Cause of Bacterial Fruit Blotch.","authors":"Soo Min Lee, Gahyeon Kim, Hun Kim, Gyung Ja Choi","doi":"10.5423/PPJ.OA.08.2024.0122","DOIUrl":"10.5423/PPJ.OA.08.2024.0122","url":null,"abstract":"Acidovorax citrulli is a causative pathogen for bacterial fruit blotch (BFB) in Cucurbitaceae, including watermelon. The most effective method to control this plant disease is to cultivate resistant cultivars. Herein, this study aimed to establish an efficient screening method to determine the resistance of watermelon cultivars against A. citrulli. To this end, we explored the virulence of seven A. citrulli isolates belonging to clonal complex group I or II based on gltA gene analysis. Furthermore, we evaluated the BFB occurrence in the seedlings of two arbitrarily selected watermelon cultivars according to the growth stage of the watermelon seedlings, inoculum concentration, and incubation temperature after inoculation in a humidity chamber. Taken together, we established the following method to determine the resistance of watermelon cultivars against A. citrulli: watermelon seedlings at the fully expanded two-leaf stage can be spray-inoculated with an A. citrulli bacterial suspension at a concentration of 1.0 × 106 cfu/ml; after incubation for 48 h at 28℃ in a humidity chamber, the plants were cultivated in a growth chamber at 25℃ with 80% relative humidity under a 12-h light/dark cycle; and BFB occurrence on the plants can be investigated at 7 dpi by visual estimation of the diseased leaf area (%). Based on these experimental methods, we investigated the resistance degree of 43 commercial watermelon cultivars against A. citrulli KACC 17005. Therefore, our results can provide important information for the development of resistant cultivars against A. citrulli.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"593-602"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel and Advanced Diagnostic Approach toward Paracidovorax citrulli Causing Bacterial Fruit Blotch in Watermelon by Direct SYBR Green Real-Time PCR Assay. 直接SYBR绿色实时荧光定量PCR法诊断西瓜细菌性果斑病的新方法

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.08.2024.0125

Hyeonheui Ham, Bang Wool Lee, Kyongnim Kim, Woohyung Lee, Yong Hwan Lee, Dong Suk Park

{"title":"A Novel and Advanced Diagnostic Approach toward Paracidovorax citrulli Causing Bacterial Fruit Blotch in Watermelon by Direct SYBR Green Real-Time PCR Assay.","authors":"Hyeonheui Ham, Bang Wool Lee, Kyongnim Kim, Woohyung Lee, Yong Hwan Lee, Dong Suk Park","doi":"10.5423/PPJ.OA.08.2024.0125","DOIUrl":"10.5423/PPJ.OA.08.2024.0125","url":null,"abstract":"Bacterial fruit blotch (BFB) caused by Paracidovorax citrulli is a devastating disease in cucurbit hosts such as watermelon. P. citrulli is a seed-borne pathogen, and contaminated seeds are the primary inoculum. Because it is difficult to control BFB after the emergence of the disease, it is essential to detect the pathogen and remove infected plant materials, including seeds, in the early stages. In this study, we developed a direct SYBR Green real-time polymerase chain reaction (PCR) method using a new species-specific marker for detecting P. citrulli with high specificity and sensitivity. The genome information of P. citrulli and related species was collected and compared to retrieve the P. citrulli-specific gene. The primer set RS01560-164 was designed based on the selected gene and tested for specificity and sensitivity using cloned DNA, genomic DNA, cell suspension, and suspensions obtained from infected watermelon cotyledons. Our primer set detected only P. citrulli from the closely related species with a detection limit of cloned DNA at 1.46 × 103 copies/µl, gDNA at 500 fg/µl, and cell suspension at 1.4 × 104 cfu/ml by real-time PCR. Our method also detected P. citrulli from diseased plants without the need for a DNA extraction phase. Therefore, the primer set and real-time PCR methods newly developed in this study could be applied for the specific detection of P. citrulli in plants or seeds with high sensitivity and robustness, providing the potential to manage BFB in cucurbits.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"671-680"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Antifungal Activity-Guided Analysis of Actinostemma lobatum Extracts through Serial Sub-fractions. 勘误：通过系列亚组分对放线素草提取物的抗真菌活性进行指导分析。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.ER.11.2023.0152

Seonwoo Choi, Song Hee Lee, Buyng Su Hwang, Young Taek Oh, Junhyun Jeon

引用次数: 0

Development of a Multiplex PCR to Simultaneously Detect Four Problematic Pathogens in Cymbidium kanran. 同时检测坎然蕙兰四种问题病原菌的多重PCR方法的建立。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.07.2024.0101

Jiwon Kim, Sunyung Yoon, Seungtae Kang, Ho Bang Kim, Kyung-Hwan Boo

{"title":"Development of a Multiplex PCR to Simultaneously Detect Four Problematic Pathogens in Cymbidium kanran.","authors":"Jiwon Kim, Sunyung Yoon, Seungtae Kang, Ho Bang Kim, Kyung-Hwan Boo","doi":"10.5423/PPJ.OA.07.2024.0101","DOIUrl":"10.5423/PPJ.OA.07.2024.0101","url":null,"abstract":"Colletotrichum gloeosporioides (Cg), Pestalotiopsis sp. (Ps), Fusarium oxysporum (Fo), and Fusarium proliferatum (Fp) are pathogens that cause various diseases of Cymbidium kanran. Species identification based on the morphological characteristics of pathogen-infected orchids is very complex and difficult; therefore, specific and reliable diagnostic methods are needed. Here, we developed a multiplex polymerase chain reaction (PCR) assay for simultaneous detection of Cg, Ps, Fo, and Fp. Four pairs of pathogen-specific primers were designed based on the internal transcribed spacer sequences of each pathogen, generating fragments of 480, 407, 321, and 279 bp, respectively. The multiplex PCR assay using these four pairs of mixed primers effectively detected the four pathogens simultaneously, with a sensitivity of 0.1 ng genomic DNA/pathogen. Application of the optimized multiplex PCR assay to symptomatic C. kanran leaf samples effectively detected single or multiple pathogens. These results indicate that the multiplex PCR developed in this study is not only a rapid and reliable method for detecting four problematic pathogens in C. kanran, but also serves as a very useful tool for early diagnosis.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"569-577"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different Infection Structures and Point Mutation of Diaporthe citri Showing Resistant against Systemic Fungicides. 柑桔不同侵染结构及点突变对系统杀菌剂的抗性。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.07.2024.0112

Zar Zar Soe, Yong Ho Shin, Yong Chull Jeun

{"title":"Different Infection Structures and Point Mutation of Diaporthe citri Showing Resistant against Systemic Fungicides.","authors":"Zar Zar Soe, Yong Ho Shin, Yong Chull Jeun","doi":"10.5423/PPJ.OA.07.2024.0112","DOIUrl":"10.5423/PPJ.OA.07.2024.0112","url":null,"abstract":"Infection behaviors of some susceptible and resistant Diaporthe citri isolates against two fungicides such as benomyl and kresoxim-methyl were observed on citrus leaves. On the chemically unsprayed leaves, there were no differences in not only the conidial germination rate but also hyphal growth between the resistant and susceptible isolates. However, on the benomyl or kresoximmethyl-pretreated leaves, both conidial germination rate and hyphal growth of the resistant isolates were not decreased, which was different with those of the susceptible isolates. These unchanging germination rate and hyphal growth of the resistant isolates, resulting in the lack of chemical efficacy, coincided with the previous studies in which strong infection was observed by the resistant isolates after pre-treatment with each chemical. On the other hand, a point mutation occurred at 198th amino acid of β-tubulin gene in the resistant isolate NEL21-2 which may cause the resistant against benomyl. However, no mutation was found in cytochrome b in the resistant isolates indicating other genomic changes may be responsible for the resistant against kresoxim-methyl.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"603-614"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0