Plant Pathology Journal最新文献

Transcriptome Analysis of Dryadomyces quercus-mongolicae, a Fungus Associated with Korean Oak Wilt Disease that Causes Oak Mortality in South Korea. 与导致韩国橡树死亡的韩国栎树枯萎病相关的真菌——蒙古栎树枯萎菌的转录组分析

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.NT.08.2024.0120

Hyowon Park, Jorge R Ibarra Caballero, Jane E Stewart, Ned B Klopfenstein, Uk Lee, Mee-Sook Kim

{"title":"Transcriptome Analysis of Dryadomyces quercus-mongolicae, a Fungus Associated with Korean Oak Wilt Disease that Causes Oak Mortality in South Korea.","authors":"Hyowon Park, Jorge R Ibarra Caballero, Jane E Stewart, Ned B Klopfenstein, Uk Lee, Mee-Sook Kim","doi":"10.5423/PPJ.NT.08.2024.0120","DOIUrl":"10.5423/PPJ.NT.08.2024.0120","url":null,"abstract":"Korean oak wilt disease associated with Dryadomyces quercus-mongolicae recently emerged as a major tree disease in South Korea. A comprehensive transcriptome analysis is presented for D. quercus-mongolicae grown in vitro on three different culture media, identifying nearly 7,000 expressed transcripts. Most transcripts are associated with proteins essential for fungal survival and growth. The 40S ribosomal protein S25, ceramide very long chain fatty acid hydroxylase, Epl1 protein, and ADP/ATP translocase are particularly important due to their critical roles in the metabolism and environmental adaptation of fungi. Gene ontology analyses revealed that 39.4%, 61.2%, and 43.3% of transcripts were successfully annotated to biological process, molecular functions, and cellular component aspects, respectively. Furthermore, key metabolic pathways were elucidated, including sphingolipid metabolism, L-tryptophan biosynthesis, and glycolysis, which provide important information on physiological functioning of D. quercus-mongolicae. Overall, these findings provide key information on fundamental biological mechanisms of D. quercus-mongolicae.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"409-418"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering Functions of a Putative Histidinol Dehydrogenase in Acidovorax citrulli by Phenotypic and Proteomic Analyses. 用表型和蛋白质组学分析解读瓜酸卵黄中一个假定的组氨酸二醇脱氢酶的功能。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.03.2025.0036

Yongmin Cho, Haerim Rhyu, Suhyun Lee, Dohyun Kim, Dae Sung Kim, Jisun H J Lee, Sang-Wook Han

{"title":"Deciphering Functions of a Putative Histidinol Dehydrogenase in Acidovorax citrulli by Phenotypic and Proteomic Analyses.","authors":"Yongmin Cho, Haerim Rhyu, Suhyun Lee, Dohyun Kim, Dae Sung Kim, Jisun H J Lee, Sang-Wook Han","doi":"10.5423/PPJ.OA.03.2025.0036","DOIUrl":"10.5423/PPJ.OA.03.2025.0036","url":null,"abstract":"Acidovorax citrulli (Ac) is a Gram-negative phytopathogenic bacterium causing bacterial fruit blotch (BFB) on cucurbit crops, specifically in the watermelon industry. However, cultivars of watermelon that are resistant to Ac have not been identified. Therefore, virulence factors/mechanisms in Ac must be characterized to develop alternative control strategies. Functions of a histidinol dehydrogenase, which is an essential enzyme for histidine biosynthesis, remain elusive in Ac. This study aims to elucidate the roles of histidinol dehydrogenase in Ac (HisDAc) using phenotype assays and proteomic analysis. The virulence of a mutant lacking a histidinol dehydrogenase, hisDAc:Tn5(EV), was diminished in geminated-seed inoculation and leaf infiltration assays, and the bacterium was impossible to grow without histidine in minimal media. However, treatment with exogenous histidine completely restored the virulence of the mutant on watermelon and its growth in minimal media, demonstrating that HisDAc is required for histidine biosynthesis, which contributes to virulence and growth. The comparative proteomic analysis indicates that HisDAc is involved in not only amino acid metabolism but also other biological mechanisms, including cell wall/membrane/envelope functions. This suggests that HisDAc may have pleiotropic effects. It was also confirmed that when Escherichia coli was incubated with Ac strains in water, the population level of E. coli increased in the presence of the mutant but not in the presence of the wild-type. This study leads to new insights regarding enzymes related to the production of primary metabolites and provides a promising target to discover an anti-virulence reagent to control BFB.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"341-351"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol Potential and Growth-Promoting Effects of Freshwater Trichoderma Strains against Plant Pathogenic Fungi in Red Pepper. 淡水木霉菌株对红辣椒植物病原真菌的生防潜力及促生作用

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.02.2025.0019

Yunjeong Heo, Gil Han, Hye Yeon Mun, Chang Soo Lee, Wonsu Cheon

{"title":"Biocontrol Potential and Growth-Promoting Effects of Freshwater Trichoderma Strains against Plant Pathogenic Fungi in Red Pepper.","authors":"Yunjeong Heo, Gil Han, Hye Yeon Mun, Chang Soo Lee, Wonsu Cheon","doi":"10.5423/PPJ.OA.02.2025.0019","DOIUrl":"10.5423/PPJ.OA.02.2025.0019","url":null,"abstract":"The aim of this study was to investigate the biocontrol potential of Trichoderma spp. against plant pathogenic fungi. Forty-four Trichoderma strains isolated from freshwater environments were evaluated for their biocontrol potential against Phytophthora capsici and Fusarium solani, as well as for their siderophore production, phosphate solubilization, and enzymatic activities. Seven Trichoderma strains showed excellent performance and were selected for further experiments. These strains were identified as T. longibrachiatum and T. capillare based on the internal transcribed spacer and translational elongation factor 1-alpha gene sequences. The selected strains demonstrated strong antifungal activity against six fungal pathogens in dual-culture and volatile organic compound (VOC) assays. Strain FBCC-F1645 exhibited particularly high antifungal activity and completely inhibited the growth of P. capsici in the VOC assay. All the selected strains significantly enhanced the growth parameters of red pepper seedlings, and flowering was effectively promoted in the treatment groups. Additionally, these strains demonstrated preventive effects against Fusarium oxysporum and P. capsici, the causative agents of Fusarium wilt and Phytophthora blight, respectively, achieving notable control efficacy. Notably, strain FBCC-F1547 completely inhibited wilting and exhibited a strong preventive effect against blight. In the pepper anthracnose prevention experiment, all the tested strain suspensions (diluted 100-fold and 500-fold) effectively inhibited Colletotrichum acutatum. These findings suggest that Trichoderma spp. isolated from freshwater environments have the potential to reduce chemical pesticide use and promote sustainable agriculture.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"392-408"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting the Onset Date of Cucumber Powdery Mildew Based on Growing Degree Days and Leaf Wetness Duration in Greenhouse Environment. 温室环境下基于生长度数和叶片湿润期的黄瓜白粉病发病日期预测

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.FT.01.2025.0010

Min Son, Haejun Jeong, Jin-Yong Jung, Jiwon Park, Jiyoon Park, Hoyoung Park, Jonghan Yoon, Se-Hoon Jung, Chun-Bo Sim, Kwang-Hyung Kim, Sook-Young Park

{"title":"Predicting the Onset Date of Cucumber Powdery Mildew Based on Growing Degree Days and Leaf Wetness Duration in Greenhouse Environment.","authors":"Min Son, Haejun Jeong, Jin-Yong Jung, Jiwon Park, Jiyoon Park, Hoyoung Park, Jonghan Yoon, Se-Hoon Jung, Chun-Bo Sim, Kwang-Hyung Kim, Sook-Young Park","doi":"10.5423/PPJ.FT.01.2025.0010","DOIUrl":"10.5423/PPJ.FT.01.2025.0010","url":null,"abstract":"Cucumber powdery mildew, caused by Podosphaera xanthii, can lead to significant yield losses in greenhouse cultivation. A calendar-based fungicide spray program is commonly employed by farmers, often leading to excessive spraying irrespective of disease conduciveness under certain weather conditions. Therefore, a disease model that can predict the onset of symptoms for determining when to start the first spray applications during a season is needed. This study developed a disease onset forecasting model, which uses growing degree days and leaf wetness duration as input variables, to aid the spray program for cucumber powdery mildew in the greenhouse environment. The model was calibrated using disease onset dates and corresponding weather data collected from two consecutive greenhouse experiments in 2022. As a result, we successfully simulated the symptom onset date with a margin of error of 5.5 days across two validation trials in 2023 and 2024. Further improvements to the model are needed to establish a model-based fungicide program in the greenhouse environment, which can be done by securing more data from additional trials for further modification and calibration of the model.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"419-424"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induced Resistance against Pseudomonas syringae pv. lachrymans in Cucumber by Spraying Cell-Free Microalgae Supernatant. 丁香假单胞菌诱导抗性研究。喷洒无细胞微藻上清液对黄瓜生长的影响。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.FT.02.2025.0028

Sang-Moo Lee, Jin-Soo Son, Bongsoo Lee, Yong-Keun Chang, Chang-Ki Shim, Choong-Min Ryu

{"title":"Induced Resistance against Pseudomonas syringae pv. lachrymans in Cucumber by Spraying Cell-Free Microalgae Supernatant.","authors":"Sang-Moo Lee, Jin-Soo Son, Bongsoo Lee, Yong-Keun Chang, Chang-Ki Shim, Choong-Min Ryu","doi":"10.5423/PPJ.FT.02.2025.0028","DOIUrl":"10.5423/PPJ.FT.02.2025.0028","url":null,"abstract":"Chlorella is a genus of aquatic photosynthetic microalgae used in the production of dietary supplements, cosmetics, and biofuels, and also recently utilized as biological control agents or biofertilizers in agricultural supplements. Chlorella supernatant elicits induced resistance in Arabidopsis thaliana but its effects on crop plants remain largely unknown. This study tested whether application of Chlorella supernatant elicited induced resistance in cucumber (Cucumis sativus L.). Foliar application of supernatants from six microalgal strains revealed that supernatants from the high biofuel-producing strains HS2 and ABC001 elicited induced resistance against Pseudomonas syringae pv. lachrymans, which causes angular leaf spot in cucumber. In addition, spraying plants with D-lactic acid, a previously known determinant of induced resistance in the Chlorella supernatant, reduced the severity of disease caused by P. syringae pv. lachrymans in cucumber leaves by activating the salicylic acid and jasmonic acid signaling pathways. The application of Chlorella supernatant thus protects a crop plant against disease while offering a cost-effective method of recycling waste supernatant.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"321-329"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Profiling and Precise Diagnosis of Pratylenchus penetrans Infestation in Soil: A qPCR-Based Molecular Approach. 基于qpcr的土壤渗透叶青虫侵染分子分析与精确诊断

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.11.2024.0181

Karthi Natesan, Byeong-Yong Park, Hyoung-Rai Ko, Eunhwa Kim, Sohee Park, Sekeun Park

{"title":"Molecular Profiling and Precise Diagnosis of Pratylenchus penetrans Infestation in Soil: A qPCR-Based Molecular Approach.","authors":"Karthi Natesan, Byeong-Yong Park, Hyoung-Rai Ko, Eunhwa Kim, Sohee Park, Sekeun Park","doi":"10.5423/PPJ.OA.11.2024.0181","DOIUrl":"10.5423/PPJ.OA.11.2024.0181","url":null,"abstract":"Pratylenchus penetrans, an important soil pathogen, has been reported on various crops in the temperate regions of South Korea. In concern, there is an urgent need for a precise, species-specific quantitative polymerase chain reaction (qPCR) kit to detect and quantify root lesion nematodes for early pest management and to controls yield losses. The present study focuses on D2-D3 region, a known marker for molecular profiling of Pratylenchus sp. A primer set mined from the highly conserved D2-D3 region of P. penetrans was used in a SYBR green based qPCR assay. Initial examination identified P. penetrans from infested soil samples using morphological and phylogenetic analyses. The DPp7F12R primer set demonstrated significant specificity in identifying P. penetrants by both conventional polymerase chain reaction (PCR) and qPCR assays. Linear regression of serially diluted DNA from nematode and nematode inoculated soil revealed a limit of quantification of 2 picograms (r² = 0.984), while also highlighting the impact of soil inhibitors. The qPCR using the DNA from varying densities of P. penetrans inoculated in soil demonstrated a robust correlation (r² = 0.98), indicating the limit of detection down to single nematode. Primer specificity evaluation with field soil sample precisely detected only P. penetrants. Species-specific DPp7F12R facilitate the direct detection of P. penetrants from soil DNA in very shorter time. Reliability of PCR was confirmed using BLAST algorithm, which identified partial sequence of PCR amplicon (300 bp) as P. penetrants. Finally, PCR assay using DPp7F12R is crucial for early detection of P. penetrans infestations, helping improve the plant health.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"330-340"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-assessment of Taxonomy and Host Range of Colletotrichum from Korea: Focus on the C. boninense, C. spaethianum Species Complexes, and Related Taxa. 韩国炭疽菌的分类及寄主范围的再评价——以炭疽菌属C. boninense、C. spaethium种复合体及相关分类群为中心。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.01.2025.0002

Le Dinh Thao, Hyorim Choi, Donghun Kang, Anbazhagan Mageswari, Jae Sung Lee, Daseul Lee, In-Young Choi, Hyeon-Dong Shin, Seung-Beom Hong

{"title":"Re-assessment of Taxonomy and Host Range of Colletotrichum from Korea: Focus on the C. boninense, C. spaethianum Species Complexes, and Related Taxa.","authors":"Le Dinh Thao, Hyorim Choi, Donghun Kang, Anbazhagan Mageswari, Jae Sung Lee, Daseul Lee, In-Young Choi, Hyeon-Dong Shin, Seung-Beom Hong","doi":"10.5423/PPJ.OA.01.2025.0002","DOIUrl":"10.5423/PPJ.OA.01.2025.0002","url":null,"abstract":"Colletotrichum species are commonly known as important phytopathogens causing anthracnose in Korea and worldwide, with a diverse range of host plants. Colletotrichum isolates preserved in the Korean Agricultural Culture Collection (KACC) are important resources for scientific research as well as anthracnose disease management strategies in Korea. Many Colletotrichum isolates in KACC had been identified using morphological characteristics and their host plants by depositors, this could lead to inaccurate species names. In this study, 38 KACC isolates were, therefore, re-identified as 13 known species (C. boninense, C. caudasporum, C. coccodes, C. echinochloae, C. karsti, C. liriopes, C. nigrum, C. sansevieriae, C. spaethianum, C. sublineola, C. sydowii, C. truncatum, and C. zhaoqingense) and a new species candidate, based on multi-locus sequence analyses of the nuclear ribosomal internal transcribed spacers, glyceraldehyde-3-phosphate dehydrogenase (gapdh), chitin synthase 1 (chs-1), histone-3 (his3), actin (act), beta-tubulin 2 (tub2), and manganese-superoxide dismutase (sod2). Of these, C. caudasporum, C. echinochloae, and C. zhaoqingense are unrecorded species in Korea. The results also revealed 16 new host-fungus combinations in Korea, including 13 new combinations worldwide. However, the pathogenicity of the fungal species in this work on their hosts was not confirmed.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"367-379"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Tomato Yellow Leaf Curl Virus-Induced Gene Expression Pattern in Tomato and Tobacco Plants. 番茄和烟草植株中 TYLCV 诱导基因表达模式的比较。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 Epub Date: 2025-04-03 DOI: 10.5423/PPJ.OA.12.2024.0191

Chenwei Zhang, Xin Jia, Xing Han, Yuan Cheng, Xiaocong Jiao, Guiyan Fan, Tiancong Ren, Xiaoli Ren, Yueyue Cai, Xuemei Zhang, Lu Li, Hongguang Pang, Zhonglin Shang

{"title":"Comparison of Tomato Yellow Leaf Curl Virus-Induced Gene Expression Pattern in Tomato and Tobacco Plants.","authors":"Chenwei Zhang, Xin Jia, Xing Han, Yuan Cheng, Xiaocong Jiao, Guiyan Fan, Tiancong Ren, Xiaoli Ren, Yueyue Cai, Xuemei Zhang, Lu Li, Hongguang Pang, Zhonglin Shang","doi":"10.5423/PPJ.OA.12.2024.0191","DOIUrl":"10.5423/PPJ.OA.12.2024.0191","url":null,"abstract":"Tomato yellow leaf curl virus (TYLCV) is a devastating pathogen that causes substantial yield losses, and this virus can infect both tomatoes (Solanum lycopersicum L.) and tobacco (Nicotiana benthamiana). In this study, a constructed infectious clone of TYLCV was used for the exploration of tomato and tobacco plants' response to virus infection. Infected plants exhibit typical symptoms of TYLCV, including leaf chlorosis, curling, and plant dwarfing. Reactive oxygen species accumulated, and severe cell necrosis appeared in the tomatoes and tobacco that were infected. After TYLCV infection, 6,775 and 900 genes' expressions were up-regulated in tomatoes and tobacco, including MYB and MADS-box transcription factors, serine/threonine protein kinase, heat shock proteins, cytochrome P450s, E3 ubiquitin-protein ligase, RAV transcription factors. Several stress-responsive kinases involved in autophagy were significantly up-regulated in tobacco but not in tomato. Moreover, silencing the RAV transcription factor, which is associated with the salicylic acid induced antiviral signaling, led to decreased virus abundance in tomato leaves. The results are helpful for an in-depth understanding of plants' resistance to TYLCV infection.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":" ","pages":"293-310"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and Nutritional Dynamics of SynCom in Suppressing Apple Fire Blight. SynCom抑制苹果火枯病的遗传和营养动态。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.03.2025.0040

Yejin Lee, Youn Min Ko, Youn-Sig Kwak

{"title":"Genetic and Nutritional Dynamics of SynCom in Suppressing Apple Fire Blight.","authors":"Yejin Lee, Youn Min Ko, Youn-Sig Kwak","doi":"10.5423/PPJ.OA.03.2025.0040","DOIUrl":"10.5423/PPJ.OA.03.2025.0040","url":null,"abstract":"Fire blight disease, caused by Erwinia amylovora, occurs in apples and other Rosaceae plants and is known to cause significant economic damage. The pathogen usually infects flowers during the reproductive growth period of plants, colonizes, and penetrates by producing exopolysaccharides in the stigma. A synthetic microbial community (SynCom) is an artificial community of microorganisms designed to enhance host viability. To construct SynCom, we attempted to identify and utilize the microbial characteristics of apple trees that are not infected with the pathogen compared to those that are infected. In our previous study, we composed SynCom with strains expected to reduce the density of fire blight pathogens through microbiome analysis, strain isolation, and continuous replacement culture. We are able to observe the disease control effect of the constructed SynCom. However, no study has been conducted to clearly determine the genetic mechanism underlying this effect of the SynCom. Here, we present that potential secondary metabolite candidates and nutritional competition with the pathogen were confirmed as biochemical mechanisms through whole genome analysis of SynCom strains. Additionally, by co-cultivating SynCom with the pathogen in limited nutrient conditions, such as apple blossom extracts, which are susceptible to the pathogen, we confirmed the potential of SynCom treatment to reduce the pathogen densities. This study demonstrates that genetic selection using metagenomics can effectively identify microorganisms with potential functional capabilities.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"380-391"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virome Analysis Deciphers the First Virus Occurrence in Melothria scabra, Revealing Two Potyviruses, Including a Highly Divergent Zucchini Yellow Mmosaic Virus Isolate. 病毒组分析破译了首次出现在黄花椒中的病毒，揭示了两种potyvirus，包括一种高度分化的西葫芦黄花叶病毒分离物。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.12.2024.0193

Li-Juan Zhu, Jianhong Xing, Jingke Li, Weifan Lin, Yubin Chi, Jun Su, Juncheng Zhang, Zhongtian Xu

{"title":"Virome Analysis Deciphers the First Virus Occurrence in Melothria scabra, Revealing Two Potyviruses, Including a Highly Divergent Zucchini Yellow Mmosaic Virus Isolate.","authors":"Li-Juan Zhu, Jianhong Xing, Jingke Li, Weifan Lin, Yubin Chi, Jun Su, Juncheng Zhang, Zhongtian Xu","doi":"10.5423/PPJ.OA.12.2024.0193","DOIUrl":"10.5423/PPJ.OA.12.2024.0193","url":null,"abstract":"Melothria scabra, an annual vine plant belonging to the family Cucurbitaceae, is usually found as a weed in agricultural ecosystems, making it a potential reservoir for crop viruses. Nonetheless, no plant virus has been documented to infect M. scabra to date. In the present study, M. scabra leaves with plant virus disease symptoms were sampled and subjected to sequencing through metatranscriptome and small RNA methods. High-throughput data analysis revealed the presence of two potyvirus species, zucchini tigre mosaic virus (ZTMV) and zucchini yellow mosaic virus (ZYMV), which were subsequently confirmed through reverse transcription PCR (RT-PCR) detection. The complete genome sequences of ZTMV and ZYMV in M. scabra, designated as ZTMV-ms (PQ720520) and ZYMV-ms (PQ720521), were determined by a combination of RT-PCR, rapid amplification of cDNA ends and Sanger sequencing. The full-genome length of ZTMV-ms and ZYMV-ms is 10,331 nt and 9,602 nt, respectively, excluding the 3' poly(A) tail. Notably, ZYMV-ms showed 80.15% similarity to its best BLASTn hit (AJ515911.1, ZYMV-WM), approaching the threshold for defining new Potyvirus species, thus classifying ZYMV-ms as a highly divergent ZYMV isolate. Both ZTMV-ms and ZYMV-ms show typical virus-derived small interfering RNA (vsiRNAs) characteristics of plant viruses, with 21- and 22-nt vsiRNAs, the latter being the most abundant, a feature rare among plant viruses. These findings provide new insights into the diversity of plant host antiviral RNAi response, as well as the evolution and host expansion of ZTMV and ZYMV, with implications for virus prevention and control.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"280-292"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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