Proteomic Analysis of a Leaf Spot Pathogen, Xanthomonas euvesicatoria pv. euvesicatoria 173 upon Sublethal Dose of Cinnamon Essential Oil.

IF 2.5 3区农林科学 Q2 PLANT SCIENCES

Plant Pathology Journal Pub Date : 2025-10-01 DOI:10.5423/PPJ.NT.05.2025.0067

Hyo-Song Nam, Yu-Na Kim, Jeong-Yong Cho, Young Cheol Kim

{"title":"Proteomic Analysis of a Leaf Spot Pathogen, Xanthomonas euvesicatoria pv. euvesicatoria 173 upon Sublethal Dose of Cinnamon Essential Oil.","authors":"Hyo-Song Nam, Yu-Na Kim, Jeong-Yong Cho, Young Cheol Kim","doi":"10.5423/PPJ.NT.05.2025.0067","DOIUrl":null,"url":null,"abstract":"<p><p>Cinnamon essential oil (CEO) exhibits antimicrobial activity in a dose-dependent manner, offering potential for controlling plant bacterial diseases. This study investigated the effects of CEO on Xanthomonas euvesicatoria pv. euvesicatoria 173, the causal agent of pepper bacterial spot disease. Treatment with an EC50 dose of CEO significantly altered bacterial culturability and protein expression profiles. Proteomic analyses revealed that, relative to untreated control, a TonB-dependent receptor and a Clp protease were upregulated, and ATP-binding cassette transporter proteins were downregulated in the CEO-exposed X. euvesicatoria. Quantitative real-time PCR confirmed that TonB and Clp proteases abundance positively correlated with transcriptional upregulation of their corresponding genes. Our findings suggest that CEO disrupts cellular homeostasis by targeting key membrane functions. Further investigation is required to elucidate how key changes in transport and proteolytic systems contribute to the control by CEO of the plant pathogen, X. euvesicatoria.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 5","pages":"699-707"},"PeriodicalIF":2.5000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Pathology Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5423/PPJ.NT.05.2025.0067","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Cinnamon essential oil (CEO) exhibits antimicrobial activity in a dose-dependent manner, offering potential for controlling plant bacterial diseases. This study investigated the effects of CEO on Xanthomonas euvesicatoria pv. euvesicatoria 173, the causal agent of pepper bacterial spot disease. Treatment with an EC50 dose of CEO significantly altered bacterial culturability and protein expression profiles. Proteomic analyses revealed that, relative to untreated control, a TonB-dependent receptor and a Clp protease were upregulated, and ATP-binding cassette transporter proteins were downregulated in the CEO-exposed X. euvesicatoria. Quantitative real-time PCR confirmed that TonB and Clp proteases abundance positively correlated with transcriptional upregulation of their corresponding genes. Our findings suggest that CEO disrupts cellular homeostasis by targeting key membrane functions. Further investigation is required to elucidate how key changes in transport and proteolytic systems contribute to the control by CEO of the plant pathogen, X. euvesicatoria.

查看原文本刊更多论文

一株叶斑病病原菌黄单胞菌的蛋白质组学分析。肉桂精油的亚致死剂量

肉桂精油（CEO）具有剂量依赖性的抗菌活性，具有控制植物细菌性病害的潜力。本文研究了CEO对黄单胞菌的抑制作用。辣椒细菌性斑疹病病原菌Euvesicatoria 173。EC50剂量的CEO显著改变了细菌培养能力和蛋白表达谱。蛋白质组学分析显示，相对于未处理的对照组，暴露于ceo的褐皮蝇中tonb依赖性受体和Clp蛋白酶上调，atp结合盒转运蛋白下调。实时荧光定量PCR证实，TonB和Clp蛋白酶丰度与其对应基因的转录上调呈正相关。我们的研究结果表明，CEO通过靶向关键的膜功能来破坏细胞稳态。需要进一步的研究来阐明运输和蛋白水解系统的关键变化是如何促进CEO控制植物病原体X. euvesicatoria的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊