Managing Plant Viruses in Tissue-Cultured Apple and Grapevine: Strategies for Detection and Eradication.

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Chae-Min Kang, Min-Jae Kim, Jin-Sung Hong, Rae-Dong Jeong
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引用次数: 0

Abstract

Producing virus-free planting materials is fundamental to sustainable fruit tree cultivation, particularly for high-value crops such as apple (Malus domestica) and grapevine (Vitis vinifera). Given the systemic and persistent nature of most plant viruses and viroids, effective elimination remains a major challenge within a tissue culture-based propagation system. Therefore, this review aims to provide a comprehensive overview of conventional virus elimination strategies-including thermotherapy, meristem and shoot tip culture, chemotherapy, and electrotherapy-while highlighting their respective strengths and limitations. Concurrently, advancements in virus detection technologies have significantly enhanced the sensitivity, speed, and precision of virus indexing, enabling the early detection of low-titer or latent infections in plantlets cultured in vitro. Besides eradication strategies, increasing attention is directed toward virus inhibition approaches. RNA interferencebased methods and plant-derived antiviral agents demonstrate promising antiviral activity in tissue-cultured apples and grapevines, offering chemical-free and ecofriendly alternatives. These biologically based inhibition strategies are particularly well-suited for integration into existing micropropagation systems. Collectively, this review emphasizes the importance of combining conventional sanitation methods with next-generation diagnostics and innovative biological inhibition technologies to develop robust, scalable, and sustainable protocols for virus-free certification.

组织培养苹果和葡萄藤的植物病毒管理:检测和根除策略。
生产无病毒种植材料是可持续果树种植的基础,特别是对苹果(Malus domestica)和葡萄藤(Vitis vinifera)等高价值作物。鉴于大多数植物病毒和类病毒的系统性和持久性,有效消除仍然是基于组织培养的繁殖系统的主要挑战。因此,本文旨在全面概述传统的病毒消除策略,包括热疗法、分生组织和茎尖培养、化疗和电疗,同时突出各自的优势和局限性。同时,病毒检测技术的进步大大提高了病毒索引的灵敏度、速度和精度,从而能够在体外培养的植株中早期发现低滴度或潜伏感染。除根除策略外,人们越来越关注病毒抑制方法。基于RNA干扰的方法和植物源抗病毒药物在组织培养的苹果和葡萄藤中显示出有希望的抗病毒活性,提供了无化学物质和环保的替代品。这些基于生物学的抑制策略特别适合整合到现有的微繁殖系统中。总的来说,这篇综述强调了将传统卫生方法与下一代诊断和创新的生物抑制技术相结合的重要性,以开发强大的、可扩展的和可持续的无病毒认证协议。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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