稻瘟病菌核效应分子MoHTR2的核定位序列分析。

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Plant Pathology Journal Pub Date : 2025-10-01 Epub Date: 2025-09-24 DOI:10.5423/PPJ.OA.06.2025.0080
Jiwon Choi, You-Jin Lim, Yong-Hwan Lee
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引用次数: 0

摘要

植物病原真菌通过分泌与宿主核酸和宿主细胞核内蛋白质相互作用的核效应物来调节宿主免疫。众所周知,核效应物具有核定位序列(NLS),可以通过α介导的经典输入途径或非经典途径进入宿主细胞核。然而,NLS中的保守基序和真菌核效应物成功核转运背后的机制在很大程度上仍未被探索。MoHTRs是稻瘟病菌的核效应物,可对宿主免疫相关基因的转录进行重编程。最近的研究表明,MoHTR1需要一个经典的NLS才能输入α-介导的进入宿主细胞核和m.o ryzae的致病性。然而,其他真菌核效应物的NLS,如MoHTR2,需要进一步研究。在本研究中,我们报道了MoHTR2不与水稻输入αs或βs相互作用。通过连续截断和定点诱变,我们确定53HH54是MoHTR2核定位所必需的核心NLS基序。我们还发现M. oryzae核效应候选物MGG_13063的双组氨酸参与了其核定位。MoHTR2核心NLS的缺失减少了m.o ryzae侵袭性菌丝的生长和病变的形成。这些发现增强了我们对真菌核效应物核定位的分子机制及其在致病性中的作用的理解,有助于更广泛地了解宿主-病原体相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unraveling the Nuclear Localization Sequence of MoHTR2, the Nuclear Effector of Magnaporthe oryzae.

Plant pathogenic fungi modulate host immunity by secreting nuclear effectors that interact with host nucleic acids and proteins within the host nucleus. Nuclear effectors are widely known to possess a nuclear localization sequence (NLS) that allows them to enter the host nucleus through either the classical importin α-mediated or non-classical pathways. However, the conserved motif in NLS and the mechanism behind successful nuclear trafficking of fungal nuclear effectors remain largely unexplored. MoHTRs, the nuclear effectors of Magnaporthe oryzae, reprogram the transcription of host immunity-associated genes. Recent research has demonstrated that MoHTR1 requires a classical NLS for importin α-mediated entry into the host nucleus and towards the pathogenicity of M. oryzae. However, the NLS of other fungal nuclear effectors, such as MoHTR2, needs further investigation. In this study, we report that MoHTR2 does not interact with rice importin αs or βs. By performing serial truncation and site-directed mutagenesis, we identified 53HH54 as the core NLS motif essential for the nuclear localization of MoHTR2. We also found that the double histidine in MGG_13063, a nuclear effector candidate of M. oryzae, is involved in its nuclear localization. Deletion of the MoHTR2 core NLS reduced the invasive hyphal growth and lesion formation by M. oryzae. These findings enhance our understanding of the molecular mechanisms underlying the nuclear localization of fungal nuclear effectors and their roles in pathogenicity, contributing to a broader understanding of host-pathogen interactions.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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