Plant Pathology Journal最新文献_第10页

Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum. 对禾谷镰刀菌中作为包囊形成诱导潜在信号的气生菌丝崩溃过程中特异表达的基因进行功能分析

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.FT.01.2024.0007

Yun-Seon Choi, Da-Woon Kim, Sung-Hwan Yun

{"title":"Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum.","authors":"Yun-Seon Choi, Da-Woon Kim, Sung-Hwan Yun","doi":"10.5423/PPJ.FT.01.2024.0007","DOIUrl":"10.5423/PPJ.FT.01.2024.0007","url":null,"abstract":"Fusarium graminearum, the causal agent of Fusarium head blight (FHB) in cereal crops, employs the production of sexual fruiting bodies (perithecia) on plant debris as a strategy for overwintering and dissemination. In an artificial condition (e.g., carrot agar medium), the F. graminearum Z3643 strain was capable of producing perithecia predominantly in the central region of the fungal culture where aerial hyphae naturally collapsed. To unravel the intricate relationship between natural aerial hyphae collapse and sexual development in this fungus, we focused on 699 genes differentially expressed during aerial hyphae collapse, with 26 selected for further analysis. Targeted gene deletion and quantitative real-time PCR analyses elucidated the functions of specific genes during natural aerial hyphae collapse and perithecium formation. Furthermore, comparative gene expression analyses between natural collapse and artificial removal conditions reveal distinct temporal profiles, with the latter inducing a more rapid and pronounced response, particularly in MAT gene expression. Notably, FGSG_09210 and FGSG_09896 play crucial roles in sexual development and aerial hyphae growth, respectively. Taken together, it is plausible that if aerial hyphae collapse occurs on plant debris, it may serve as a physical cue for inducing perithecium formation in crop fields, representing a survival strategy for F. graminearum during winter. Insights into the molecular mechanisms underlying aerial hyphae collapse provides offer potential strategies for disease control against FHB caused by F. graminearum.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"83-97"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Antagonistic Bacillus against Brown Rot in Dendrocalamus latiflorus and Preparation of Applying Bacterial Suspension. 筛选抗扁柏褐腐病的拮抗芽孢杆菌并制备应用细菌悬浮液

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.07.2023.0107

Fengying Luo, Hang Chen, Wenjian Wei, Han Liu, Youzhong Chen, Shujiang Li

{"title":"Screening of Antagonistic Bacillus against Brown Rot in Dendrocalamus latiflorus and Preparation of Applying Bacterial Suspension.","authors":"Fengying Luo, Hang Chen, Wenjian Wei, Han Liu, Youzhong Chen, Shujiang Li","doi":"10.5423/PPJ.OA.07.2023.0107","DOIUrl":"10.5423/PPJ.OA.07.2023.0107","url":null,"abstract":"The aim of this study was to isolate biocontrol bacteria that could antagonize brown rot of Dendrocalamus latiflorus, optimize the culture conditions, and develop an effective biocontrol preparation for brown rot of D. latiflorus. This study isolated a bacterium with an antagonistic effect on bamboo brown rot from healthy D. latiflorus rhizosphere soil. Morphology, molecular biology, and physiological biochemistry methods identified it as Bacillus siamensis. The following culturing media and conditions improved the inhibition effect of B. siamensis: the best culturing media were 2% sucrose, 1.5% yeast extract, and 0.7% potassium chloride; the optimal culturing time, temperature, pH, and inoculation amount were 48 h, 30℃, 6, and 20%. The optimum formula of the applying bacterial suspension was 14% sodium dodecyl benzene sulfonate emulsifier, 4% Na2HPO4·2H2O, 0.3% hydroxypropyl methylcellulose thickener, and 20% B. siamensis. The pot experiment results showed the control effect of applying bacterial suspension, diluted 1,000 times is still better than that of 24% fenbuconazole suspension. The applying bacterial suspension enables reliable control of brown rot in D. latiflorus.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"1-15"},"PeriodicalIF":1.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing. 利用元转录组和小 RNA 测序首次在栀子中发现的豆类常见花叶病毒分离株的分子特征。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.11.2023.0163

Zhong-Tian Xu, Hai-Tao Weng, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li, Yi-Yuan Li

{"title":"Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing.","authors":"Zhong-Tian Xu, Hai-Tao Weng, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li, Yi-Yuan Li","doi":"10.5423/PPJ.OA.11.2023.0163","DOIUrl":"10.5423/PPJ.OA.11.2023.0163","url":null,"abstract":"Gardenia (Gardenia jasminoides) is a popular and economically vital plant known for its ornamental and medicinal properties. Despite its widespread cultivation, there has been no documentation of plant viruses on gardenia yet. In the present study, gardenia leaves exhibiting symptoms of plant viral diseases were sampled and sequenced by both metatranscriptome and small RNA sequencing. As a consequence, bean common mosaic virus (BCMV) was identified in gardenia for the first time and named BCMV-gardenia. The full genome sequence of BCMV-gardenia is 10,054 nucleotides (nt) in length (excluding the poly (A) at the 3' termini), encoding a large polyprotein of 3,222 amino acids. Sequence analysis showed that the N-termini of the polyprotein encoded by BCMV-gardenia is less conserved when compared to other BCMV isolates, whereas the C-termini is the most conserved. Maximum likelihood phylogenetic analysis showed that BCMV-gardenia was clustered closely with other BCMV isolates identified outside the leguminous plants. Our results indicated that the majority of BCMV-gardenia virus-derived small interfering RNAs (vsiRNAs) were 21 nt and 22 nt, with 21 nt being more abundant. The first nucleotide at the 5' termini of vsiRNAs derived from BCMV-gardenia preferred U and A. The ratio of vsiRNAs derived from sense (51.1%) and antisense (48.9%) strands is approaching, and the distribution of vsiRNAs along the viral genome is generally even, with some hot spots forming in local regions. Our findings could provide new insights into the diversity, evolution, and host expansion of BCMV and contribute to the prevention and treatment of this virus.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"73-82"},"PeriodicalIF":1.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-identification of Colletotrichum gloeosporioides Species Complex Isolates in Korea and Their Host Plants. 重新鉴定韩国的 Colletotrichum gloeosporioides 种复合分离株及其寄主植物。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.09.2023.0133

Le Dinh Thao, Hyorim Choi, Yunhee Choi, Anbazhagan Mageswari, Daseul Lee, Dong-Hyun Kim, Hyeon-Dong Shin, Hyowon Choi, Ho-Jong Ju, Seung-Beom Hong

{"title":"Re-identification of Colletotrichum gloeosporioides Species Complex Isolates in Korea and Their Host Plants.","authors":"Le Dinh Thao, Hyorim Choi, Yunhee Choi, Anbazhagan Mageswari, Daseul Lee, Dong-Hyun Kim, Hyeon-Dong Shin, Hyowon Choi, Ho-Jong Ju, Seung-Beom Hong","doi":"10.5423/PPJ.OA.09.2023.0133","DOIUrl":"10.5423/PPJ.OA.09.2023.0133","url":null,"abstract":"The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"16-29"},"PeriodicalIF":1.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Inexpensive System for Rapid and Accurate On-site Detection of Garlic-Infected Viruses by Agarose Gel Electrophoresis Followed by Array Assay. 通过琼脂糖凝胶电泳和阵列检测快速准确地现场检测大蒜感染病毒的廉价系统。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.FT.11.2023.0146

Kazuyoshi Furuta, Shusuke Kawakubo, Jun Sasaki, Chikara Masuta

{"title":"An Inexpensive System for Rapid and Accurate On-site Detection of Garlic-Infected Viruses by Agarose Gel Electrophoresis Followed by Array Assay.","authors":"Kazuyoshi Furuta, Shusuke Kawakubo, Jun Sasaki, Chikara Masuta","doi":"10.5423/PPJ.FT.11.2023.0146","DOIUrl":"10.5423/PPJ.FT.11.2023.0146","url":null,"abstract":"Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"40-47"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Resistance Induction in Mungbean against Alternaria alternata through RNA Interference. 通过 RNA 干扰评估绿豆对交替孢霉的抗性诱导。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.03.2023.0048

Hira Abbas, Nazia Nahid, Muhammad Shah Nawaz Ul Rehman, Tayyaba Shaheen, Sadia Liaquat

{"title":"Assessment of Resistance Induction in Mungbean against Alternaria alternata through RNA Interference.","authors":"Hira Abbas, Nazia Nahid, Muhammad Shah Nawaz Ul Rehman, Tayyaba Shaheen, Sadia Liaquat","doi":"10.5423/PPJ.OA.03.2023.0048","DOIUrl":"10.5423/PPJ.OA.03.2023.0048","url":null,"abstract":"A comprehensive survey of mungbean-growing areas was conducted to observe leaf spot disease caused by Alternaria alternata. Alternaria leaf spot symptoms were observed on the leaves. Diversity of 50 genotypes of mungbean was assessed against A. alternata and data on pathological traits was subjected to cluster analysis. The results showed that genotypes of mungbean were grouped into four clusters based on resistance parameters under the influence of disease. The principal component biplot demonstrated that all the disease-related parameters (% disease incidence, % disease intensity, lesion area, and % of infection) were strongly correlated with each other. Alt a 1 gene that is precisely found in Alternaria species and is responsible for virulence and pathogenicity. Alt a 1 gene was amplified using gene specific primers. The isolated pathogen produced similar symptoms when inoculated on mungbean and tobacco. The sequence analysis of the internal transcribed spacer (ITS) region, a 600 bp fragment amplified using specific primers, ITS1 and ITS2 showed 100% identity with A. alternata. Potato virus X (PVX) -based silencing vector expressing Alt a 1 gene was constructed to control this pathogen through RNA interference in tobacco. Out of 50 inoculated plants, 9 showed delayed onset of disease. Furthermore, to confirm our findings at molecular level semi-quantitative reverse transcriptase polymerase chain reaction was used. Both phenotypic and molecular investigation indicated that RNAi induced through the VIGS vector was efficacious in resisting the pathogen in the model host, Tobacco (Nicotiana tabacum). To the best of our knowledge, this study has been reported for the first time.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"59-72"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Insights into Abies koreana Drought Tolerance Conferred by Aureobasidium pullulans AK10. Aureobasidium pullulans AK10 对韩国赤松耐旱性的转录组学启示。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.FT.11.2023.0161

Jungwook Park, Mohamed Mannaa, Gil Han, Hyejung Jung, Hyo Seong Jeon, Jin-Cheol Kim, Ae Ran Park, Young-Su Seo

{"title":"Transcriptomic Insights into Abies koreana Drought Tolerance Conferred by Aureobasidium pullulans AK10.","authors":"Jungwook Park, Mohamed Mannaa, Gil Han, Hyejung Jung, Hyo Seong Jeon, Jin-Cheol Kim, Ae Ran Park, Young-Su Seo","doi":"10.5423/PPJ.FT.11.2023.0161","DOIUrl":"10.5423/PPJ.FT.11.2023.0161","url":null,"abstract":"The conservation of the endangered Korean fir, Abies koreana, is of critical ecological importance. In our previous study, a yeast-like fungus identified as Aureobasidium pullulans AK10, was isolated and shown to enhance drought tolerance in A. koreana seedlings. In this study, the effectiveness of Au. pullulans AK10 treatment in enhancing drought tolerance in A. koreana was confirmed. Furthermore, using transcriptome analysis, we compared A. koreana seedlings treated with Au. pullulans AK10 to untreated controls under drought conditions to elucidate the molecular responses involved in increased drought tolerance. Our findings revealed a predominance of downregulated genes in the treated seedlings, suggesting a strategic reallocation of resources to enhance stress defense. Further exploration of enriched Kyoto Encyclopedia of Genes and Genomes pathways and protein-protein interaction networks revealed significant alterations in functional systems known to fortify drought tolerance, including the terpenoid backbone biosynthesis, calcium signaling pathway, pyruvate metabolism, brassinosteroid biosynthesis, and, crucially, flavonoid biosynthesis, renowned for enhancing plant drought resistance. These findings deepen our comprehension of how AK10 biostimulation enhances the resilience of A. koreana to drought stress, marking a substantial advancement in the effort to conserve this endangered tree species through environmentally sustainable treatment.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"30-39"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem Rot of Pearl Millet Prevalence, Symptomatology, Disease Cycle, Disease Rating Scale and Pathogen Characterization in Pearl Millet-Klebsiella Pathosystem. 珍珠米-克雷伯氏菌病原系统中珍珠米茎腐病的发病率、症状、病害周期、病害量表和病原特征。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.09.2023.0126

Vinod Kumar Malik, Pooja Sangwan, Manjeet Singh, Pavitra Kumari, Niharika Shoeran, Navjeet Ahalawat, Mukesh Kumar, Harsh Deep, Kamla Malik, Preety Verma, Pankaj Yadav, Sheetal Kumari, Aakash, Sambandh Dhal

{"title":"Stem Rot of Pearl Millet Prevalence, Symptomatology, Disease Cycle, Disease Rating Scale and Pathogen Characterization in Pearl Millet-Klebsiella Pathosystem.","authors":"Vinod Kumar Malik, Pooja Sangwan, Manjeet Singh, Pavitra Kumari, Niharika Shoeran, Navjeet Ahalawat, Mukesh Kumar, Harsh Deep, Kamla Malik, Preety Verma, Pankaj Yadav, Sheetal Kumari, Aakash, Sambandh Dhal","doi":"10.5423/PPJ.OA.09.2023.0126","DOIUrl":"10.5423/PPJ.OA.09.2023.0126","url":null,"abstract":"The oldest and most extensively cultivated form of millet, known as pearl millet (Pennisetum glaucum (L.) R. Br. Syn. Pennisetum americanum (L.) Leeke), is raised over 312.00 lakh hectares in Asian and African countries. India is regarded as the significant hotspot for pearl millet diversity. In the Indian state of Haryana, where pearl millet is grown, a new and catastrophic bacterial disease known as stem rot of pearl millet spurred by the bacterium Klebsiella aerogenes (formerly Enterobacter) was first observed during fall 2018. The disease appears in form of small to long streaks on leaves, lesions on stem, and slimy rot appearance of stem. The associated bacterium showed close resemblance to Klebsiella aerogenes that was confirmed by a molecular evaluation based on 16S rDNA and gyrA gene nucleotide sequences. The isolates were also identified to be Klebsiella aerogenes based on biochemical assays, where Klebsiella isolates differed in D-trehalose and succinate alkalisation tests. During fall 2021-2023, the disease has spread all the pearl millet-growing districts of the state, extending up to 70% disease incidence in the affected fields. The disease is causing considering grain as well as fodder losses. The proposed scale, consisting of six levels (0-5), is developed where scores 0, 1, 2, 3, 4, and 5 have been categorized as highly resistant, resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible disease reaction, respectively. The disease cycle, survival of pathogen, and possible losses have also been studied to understand other features of the disease.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"48-58"},"PeriodicalIF":1.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato. stercoris芽孢杆菌分离株B.PNR1作为番茄枯萎病防治和生长促进剂的评价和基因组挖掘。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.01.2023.0018

Rattana Pengproh, Thanwanit Thanyasiriwat, Kusavadee Sangdee, Juthaporn Saengprajak, Praphat Kawicha, Aphidech Sangdee

{"title":"Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato.","authors":"Rattana Pengproh, Thanwanit Thanyasiriwat, Kusavadee Sangdee, Juthaporn Saengprajak, Praphat Kawicha, Aphidech Sangdee","doi":"10.5423/PPJ.OA.01.2023.0018","DOIUrl":"10.5423/PPJ.OA.01.2023.0018","url":null,"abstract":"Recently, strategies for controlling Fusarium oxysporum f. sp. lycopersici (Fol), the causal agent of Fusarium wilt of tomato, focus on using effective biocontrol agents. In this study, an analysis of the biocontrol and plant growth promoting (PGP) attributes of 11 isolates of loamy soil Bacillus spp. has been conducted. Among them, the isolates B.PNR1 and B.PNR2 inhibited the mycelial growth of Fol by inducing abnormal fungal cell wall structures and cell wall collapse. Moreover, broad-spectrum activity against four other plant pathogenic fungi, F. oxysporum f. sp. cubense race 1 (Foc), Sclerotium rolfsii, Colletotrichum musae, and C. gloeosporioides were noted for these isolates. These two Bacillus isolates produced indole acetic acid, phosphate solubilization enzymes, and amylolytic and cellulolytic enzymes. In the pot experiment, the culture filtrate from B.PNR1 showed greater inhibition of the fungal pathogens and significantly promoted the growth of tomato plants more than those of the other treatments. Isolate B.PNR1, the best biocontrol and PGP, was identified as Bacillus stercoris by its 16S rRNA gene sequence and whole genome sequencing analysis (WGS). The WGS, through genome mining, confirmed that the B.PNR1 genome contained genes/gene cluster of a nonribosomal peptide synthetase/polyketide synthase, such as fengycin, surfactin, bacillaene, subtilosin A, bacilysin, and bacillibactin, which are involved in antagonistic and PGP activities. Therefore, our finding demonstrates the effectiveness of B. stercoris strain B.PNR1 as an antagonist and for plant growth promotion, highlighting the use of this microorganism as a biocontrol agent against the Fusarium wilt pathogen and PGP abilities in tomatoes.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"430-448"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bc/0b/ppj-oa-01-2023-0018.PMC10580056.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Damage Triggers the Activation of Immune Response to Viral Pathogens via Salicylic Acid in Plants. DNA损伤通过水杨酸触发植物对病毒病原体的免疫反应。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.08.2023.0112

Hwi-Won Jeong, Tae Ho Ryu, Hyo-Jeong Lee, Kook-Hyung Kim, Rae-Dong Jeong

{"title":"DNA Damage Triggers the Activation of Immune Response to Viral Pathogens via Salicylic Acid in Plants.","authors":"Hwi-Won Jeong, Tae Ho Ryu, Hyo-Jeong Lee, Kook-Hyung Kim, Rae-Dong Jeong","doi":"10.5423/PPJ.OA.08.2023.0112","DOIUrl":"10.5423/PPJ.OA.08.2023.0112","url":null,"abstract":"Plants are challenged by various pathogens throughout their lives, such as bacteria, viruses, fungi, and insects; consequently, they have evolved several defense mechanisms. In addition, plants have developed localized and systematic immune responses due to biotic and abiotic stress exposure. Animals are known to activate DNA damage responses (DDRs) and DNA damage sensor immune signals in response to stress, and the process is well studied in animal systems. However, the links between stress perception and immune response through DDRs remain largely unknown in plants. To determine whether DDRs induce plant resistance to pathogens, Arabidopsis plants were treated with bleomycin, a DNA damage-inducing agent, and the replication levels of viral pathogens and growth of bacterial pathogens were determined. We observed that DDR-mediated resistance was specifically activated against viral pathogens, including turnip crinkle virus (TCV). DDR increased the expression level of pathogenesis-related (PR) genes and the total salicylic acid (SA) content and promoted mitogen-activated protein kinase signaling cascades, including the WRKY signaling pathway in Arabidopsis. Transcriptome analysis further revealed that defense- and SA-related genes were upregulated by DDR. The atm-2atr-2 double mutants were susceptible to TCV, indicating that the main DDR signaling pathway sensors play an important role in plant immune responses. In conclusion, DDRs activated basal immune responses to viral pathogens.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"449-465"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/db/ppj-oa-08-2023-0112.PMC10580055.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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