Plant Pathology Journal最新文献_第9页

Light and Electron Microscopy Studies Elucidating Mechanisms of Tomato Leaf Infection by Pseudocercospora fuligena. 光镜和电镜研究阐明番茄叶片侵染机制。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-04-01 DOI: 10.5423/PPJ.OA.06.2022.0082

Zelalem Mersha, Girma Birru, Bernhard Hau

{"title":"Light and Electron Microscopy Studies Elucidating Mechanisms of Tomato Leaf Infection by Pseudocercospora fuligena.","authors":"Zelalem Mersha, Girma Birru, Bernhard Hau","doi":"10.5423/PPJ.OA.06.2022.0082","DOIUrl":"https://doi.org/10.5423/PPJ.OA.06.2022.0082","url":null,"abstract":"The fungal pathogen Pseudocercospora fuligena, known to affect tomatoes in the tropics and subtropics, has been reported from temperate climates including the United States and Turkey in recent years. In this study, an isolate from fresh tomatoes and the disease it causes were characterized and infection mechanisms investigated. Macroscopically, both sides of tomato leaves show indistinct effuse patches but prolific production of fuliginous lesions is conspicuous on the abaxial side first but also on the adaxial side later on as infection progressed. Microscopically, fascicles of conidiophores (11-128 µm × 3.5-9 µm) arising from stromata and conidia with up to 12 septations were observed. Molecular characterization of the isolate revealed high homology (99.8%) to other P. fuligena isolated from tomatoes in Turkey. Out of the 10 media tested, P. fuligena grew significantly well and sporulated better on unsealed tomato oatmeal agar and carrot leaf decoction agar, both supplemented with CaCO3. Direct transfer of conidia from profusely sporulating lesions was the easiest and quickest method of isolation for in-vitro studies. Light and scanning electron microscopy on cleared and intact tomato leaves further confirmed stomatal penetration and egress as well as prevalence of primary and secondary infection hyphae. In situ, blocked stomatal aperture areas of 154, 401, and 2,043 µm2 were recorded at 7, 12, and 17 days after inoculation, respectively. With the recent expanded horizon of the pathosystem and its consequential impact, such studies will be useful for a proper diagnosis, identification and management of the disease on tomato worldwide.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 2","pages":"181-190"},"PeriodicalIF":2.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/96/ppj-oa-06-2022-0082.PMC10102566.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability in Responses to Phoma medicaginis Infection in a Tunisian Collection of Three Annual Medicago Species. 在突尼斯收集的三种一年生紫花植物中，对药物感染反应的变异性。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-04-01 DOI: 10.5423/PPJ.OA.09.2022.0134

Mounawer Badri, Amina Ayadi, Asma Mahjoub, Amani Benltoufa, Manel Chaouachi, Rania Ranouch, Najah Ben Cheikh, Aissa Abdelguerf, Meriem Laouar, Chedly Abdelly, Ndiko Ludidi, Naceur Djébali

{"title":"Variability in Responses to Phoma medicaginis Infection in a Tunisian Collection of Three Annual Medicago Species.","authors":"Mounawer Badri, Amina Ayadi, Asma Mahjoub, Amani Benltoufa, Manel Chaouachi, Rania Ranouch, Najah Ben Cheikh, Aissa Abdelguerf, Meriem Laouar, Chedly Abdelly, Ndiko Ludidi, Naceur Djébali","doi":"10.5423/PPJ.OA.09.2022.0134","DOIUrl":"https://doi.org/10.5423/PPJ.OA.09.2022.0134","url":null,"abstract":"Spring black stem and leaf spot, caused by Phoma medicaginis, is an issue in annual Medicago species. Therefore, in this study, we analyzed the response to P. medicaginis infection in a collection of 46 lines of three annual Medicago species (M. truncatula, M. ciliaris, and M. polymorpha) showing different geographic distribution in Tunisia. The reaction in the host to the disease is explained by the effects based on plant species, lines nested within species, treatment, the interaction of species × treatment, and the interaction of lines nested within species × treatment. Medicago ciliaris was the least affected for aerial growth under infection. Furthermore, the largest variation within species was found for M. truncatula under both conditions. Principal component analysis and hierarchical classification showed that M. ciliaris lines formed a separate group under control treatment and P. medicaginis infection and they are the most vigorous in growth. These results indicate that M. ciliaris is the least susceptible in response to P. medicaginis infection among the three Medicago species investigated here, which can be used as a good candidate in crop rotation to reduce disease pressure in the field and as a source of P. medicaginis resistance for the improvement of forage legumes.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 2","pages":"171-180"},"PeriodicalIF":2.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b3/f7/ppj-oa-09-2022-0134.PMC10102567.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9360535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rapid and Simple Method for DNA Preparation of Magnaporthe oryzae from Single Rice Blast Lesions for PCR-Based Molecular Analysis. 一种快速简便的稻瘟病菌DNA制备方法及其分子分析。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-04-01 DOI: 10.5423/PPJ.ER.02.2022.0017

Liying Dong, Shufang Liu, Jing Li, Didier Tharreau, Pei Liu, Dayun Tao, Qinzhong Qinzhong

引用次数: 2

Different Response Mechanisms of Rhizosphere Microbial Communities in Two Species of Amorphophallus to Pectobacterium carotovorum subsp. carotovorum Infection. 两种魔芋根际微生物群落对胡萝卜乳杆菌的不同响应机制carotovorum感染。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-04-01 DOI: 10.5423/PPJ.OA.12.2022.0157

Min Yang, Ying Qi, Jiani Liu, Penghua Gao, Feiyan Huang, Lei Yu, Hairu Chen

{"title":"Different Response Mechanisms of Rhizosphere Microbial Communities in Two Species of Amorphophallus to Pectobacterium carotovorum subsp. carotovorum Infection.","authors":"Min Yang, Ying Qi, Jiani Liu, Penghua Gao, Feiyan Huang, Lei Yu, Hairu Chen","doi":"10.5423/PPJ.OA.12.2022.0157","DOIUrl":"https://doi.org/10.5423/PPJ.OA.12.2022.0157","url":null,"abstract":"Soft rot is a widespread, catastrophic disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) that severely damages the production of Amorphophallus spp. This study evaluated the rhizosphere bacterial and fungal communities in Pcc-infected and uninfected plants of two species of Amorphophallus, A. muelleri and A. konjac. Principal component analysis showed that the samples formed different clusters according to the Pcc infection status, indicating that Pcc infection can cause a large number of changes in the bacterial and fungal communities in the Amorphophallus spp. rhizosphere soil. However, the response mechanisms of A. muelleri and A. konjac are different. There was little difference in the overall microbial species composition among the four treatments, but the relative abundances of core microbiome members were significantly different. The relative abundances of Actinobacteria, Chloroflexi, Acidobacteria, Firmicutes, Bacillus, and Lysobacter were lower in infected A. konjac plants than in healthy plants; in contrast, those of infected A. muelleri plants were higher than those in healthy plants. For fungi, the relative abundances of Ascomycota and Fusarium in the rhizosphere of infected A. konjac plants were significantly higher than those of healthy plants, but those of infected A. muelleri plants were lower than those of healthy plants. The relative abundance of beneficial Penicillium fungi was lower in infected A. konjac plants than in healthy plants, and that of infected A. muelleri plants was higher than that of healthy plants. These findings can provide theoretical references for further functional research and utilization of Amorphophallus spp. rhizosphere microbial communities in the future.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 2","pages":"207-219"},"PeriodicalIF":2.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7d/25/ppj-oa-12-2022-0157.PMC10102568.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9307156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Salicylic Acid and N-Hydroxy-Pipecolic Acid in Systemic Acquired Resistance. 水杨酸和n -羟基胡椒酸在全身获得性耐药中的调控作用。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.RW.10.2022.0145

Gah-Hyun Lim

引用次数: 1

Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease. mec基因破坏对胡萝卜乳杆菌甲硫氨酸合成的影响。胡萝卜素Pcc21和减少软腐病。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.09.2022.0135

Seonmi Yu, Jihee Kang, Eui-Hwan Chung, Yunho Lee

{"title":"Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease.","authors":"Seonmi Yu, Jihee Kang, Eui-Hwan Chung, Yunho Lee","doi":"10.5423/PPJ.OA.09.2022.0135","DOIUrl":"https://doi.org/10.5423/PPJ.OA.09.2022.0135","url":null,"abstract":"Plant pathogenic Pectobacterium species cause severe soft rot/blackleg diseases in many economically important crops worldwide. Pectobacterium utilizes plant cell wall degrading enzymes (PCWDEs) as the main virulence determinants for its pathogenicity. In this study, we screened a random mutant, M29 is a transposon insertion mutation in the metC gene encoding cystathionine β-lyase that catalyzes cystathionine to homocysteine at the penultimate step in methionine biosynthesis. M29 became a methionine auxotroph and resulted in growth defects in methionine-limited conditions. Impaired growth was restored with exogenous methionine or homocysteine rather than cystathionine. The mutant exhibited reduced soft rot symptoms in Chinese cabbages and potato tubers, maintaining activities of PCWDEs and swimming motility. The mutant was unable to proliferate in both Chinese cabbages and potato tubers. The reduced virulence was partially restored by a complemented strain or 100 µM of methionine, whereas it was fully restored by the extremely high concentration (1 mM). Our transcriptomic analysis showed that genes involved in methionine biosynthesis or transporter were downregulated in the mutant. Our results demonstrate that MetC is important for methionine biosynthesis and transporter and influences its virulence through Pcc21 multiplication in plant hosts.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"62-74"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9f/f3/ppj-oa-09-2022-0135.PMC9929172.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10792978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Past and Future Epidemiological Perspectives and Integrated Management of Rice Bakanae in Korea. 韩国水稻白斑病的流行病学研究与综合治理。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.RW.08.2022.0123

Soobin Shin, Hyunjoo Ryu, Jin-Yong Jung, Yoon-Ju Yoon, Gudam Kwon, Nahyun Lee, Na Hee Kim, Rowoon Lee, Jiseon Oh, Minju Baek, Yoon Soo Choi, Jungho Lee, Kwang-Hyung Kim

{"title":"Past and Future Epidemiological Perspectives and Integrated Management of Rice Bakanae in Korea.","authors":"Soobin Shin, Hyunjoo Ryu, Jin-Yong Jung, Yoon-Ju Yoon, Gudam Kwon, Nahyun Lee, Na Hee Kim, Rowoon Lee, Jiseon Oh, Minju Baek, Yoon Soo Choi, Jungho Lee, Kwang-Hyung Kim","doi":"10.5423/PPJ.RW.08.2022.0123","DOIUrl":"https://doi.org/10.5423/PPJ.RW.08.2022.0123","url":null,"abstract":"In the past, rice bakanae was considered an endemic disease that did not cause significant losses in Korea; however, the disease has recently become a serious threat due to climate change, changes in farming practices, and the emergence of fungicide-resistant strains. Since the bakanae outbreak in 2006, its incidence has gradually decreased due to the application of effective control measures such as hot water immersion methods and seed disinfectants. However, in 2013, a marked increase in bakanae incidence was observed, causing problems for rice farmers. Therefore, in this review, we present the potential risks from climate change based on an epidemiological understanding of the pathogen, host plant, and environment, which are the key elements influencing the incidence of bakanae. In addition, disease management options to reduce the disease pressure of bakanae below the economic threshold level are investigated, with a specific focus on resistant varieties, as well as chemical, biological, cultural, and physical control methods. Lastly, as more effective countermeasures to bakanae, we propose an integrated disease management option that combines different control methods, including advanced imaging technologies such as remote sensing. In this review, we revisit and examine bakanae, a traditional seed-borne fungal disease that has not gained considerable attention in the agricultural history of Korea. Based on the understanding of the present significance and anticipated risks of the disease, the findings of this study are expected to provide useful information for the establishment of an effective response strategy to bakanae in the era of climate change.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"1-20"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/93/ppj-rw-08-2022-0123.PMC9929170.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10792975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Crucial Role of Chloroplast-Related Proteins in Viral Genome Replication and Host Defense against Positive-Sense Single-Stranded RNA Viruses. 叶绿体相关蛋白在病毒基因组复制和宿主防御正义单链RNA病毒中的关键作用。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.RW.10.2022.0139

John Bwalya, Kook-Hyung Kim

{"title":"The Crucial Role of Chloroplast-Related Proteins in Viral Genome Replication and Host Defense against Positive-Sense Single-Stranded RNA Viruses.","authors":"John Bwalya, Kook-Hyung Kim","doi":"10.5423/PPJ.RW.10.2022.0139","DOIUrl":"https://doi.org/10.5423/PPJ.RW.10.2022.0139","url":null,"abstract":"Plant viruses are responsible for worldwide production losses of numerous economically important crops. The most common plant RNA viruses are positivesense single-stranded RNA viruses [(+)ss RNA viruses]. These viruses have small genomes that encode a limited number of proteins. The viruses depend on their host's machinery for the replication of their RNA genome, assembly, movement, and attraction to the vectors for dispersal. Recently researchers have reported that chloroplast proteins are crucial for replicating (+)ss plant RNA viruses. Some chloroplast proteins, including translation initiation factor [eIF(iso)4E] and 75 DEAD-box RNA helicase RH8, help viruses fulfill their infection cycle in plants. In contrast, other chloroplast proteins such as PAP2.1, PSaC, and ATPsyn-α play active roles in plant defense against viruses. This is also consistent with the idea that reactive oxygen species, salicylic acid, jasmonic acid, and abscisic acid are produced in chloroplast. However, knowledge of molecular mechanisms and functions underlying these chloroplast host factors during the virus infection is still scarce and remains largely unknown. Our review briefly summarizes the latest knowledge regarding the possible role of chloroplast in plant virus replication, emphasizing chloroplast-related proteins. We have highlighted current advances regarding chloroplast-related proteins' role in replicating plant (+)ss RNA viruses.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"28-38"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f7/b9/ppj-rw-10-2022-0139.PMC9929168.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10792976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Colony Age of Trichoderma azevedoi Alters the Profile of Volatile Organic Compounds and Ability to Suppress Sclerotinia sclerotiorum in Bean Plants. 毛霉菌的菌落年龄会改变挥发性有机化合物的分布以及抑制豆类植物中硬皮病菌的能力。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.08.2022.0106

Lincon Rafael da Silva, Leonardo Luís de Barros Rodrigues, Amanda Silva Botelho, Bruna Sartório de Castro, Paulo Henrique Pereira Costa Muniz, Maria Carolina Blassioli Moraes, Sueli Corrêa Marques de Mello

{"title":"Colony Age of Trichoderma azevedoi Alters the Profile of Volatile Organic Compounds and Ability to Suppress Sclerotinia sclerotiorum in Bean Plants.","authors":"Lincon Rafael da Silva, Leonardo Luís de Barros Rodrigues, Amanda Silva Botelho, Bruna Sartório de Castro, Paulo Henrique Pereira Costa Muniz, Maria Carolina Blassioli Moraes, Sueli Corrêa Marques de Mello","doi":"10.5423/PPJ.OA.08.2022.0106","DOIUrl":"10.5423/PPJ.OA.08.2022.0106","url":null,"abstract":"Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. The occurrence of diseases, such as white mold, caused by Sclerotinia sclerotiorum can limit the production of this legume. The use of Trichoderma has become an important strategy in the suppression of this disease. The aim of the present study was to evaluate the effect of volatile organic compounds (VOCs) emitted by Trichoderma azevedoi CEN1241 in five different growth periods on the severity of white mold in common bean. The in vitro assays were carried out in double-plate and split-plate, and the in vivo assays, through the exposure of the mycelia of S. sclerotiorum to the VOCs of T. azevedoi CEN1241 and subsequent inoculation in bean plants. Chemical analysis by gas chromatography coupled to mass spectrometry detected 37 VOCs produced by T. azevedoi CEN1241, covering six major chemical classes. The profile of VOCs produced by T. azevedoi CEN1241 varied according to colony age and was shown to be related to the ability of the biocontrol agent to suppress S. sclerotiorum. T. azevedoi CEN1241 VOCs reduced the size of S. sclerotiorum lesions on bean fragments in vitro and reduced disease severity in a greenhouse. This study demonstrated in a more applied way that the mechanism of antibiosis through the production of volatile compounds exerted by Trichoderma can complement other mechanisms, such as parasitism and competition, thus contributing to a better efficiency in the control of white mold in bean plants.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"39-51"},"PeriodicalIF":1.8,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/47/ppj-oa-08-2022-0106.PMC9929164.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9287283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay. 逆转录重组酶聚合酶扩增联合横向流动免疫法快速可靠检测蕙兰花叶病毒的应用

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.ER.10.2022.0147

Do-Hyun Kim, Rae-Dong Jeong, Sena Choi, Ho-Jong Ju, Ju-Yeon Yoon

引用次数: 0