Plant Pathology Journal最新文献_第9页

High-Throughput Sequencing Identified Multiple Fig Viruses and Viroids Associated with Fig Mosaic Disease in Iraq. 高通量测序鉴定出与伊拉克无花果马赛克病有关的多种无花果病毒和病毒株。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.04.2024.0068

Nabeel Al-Kaeath, Shrooq Zagier, Osamah Alisawi, Fadhal Al Fadhal, Naima Mahfoudhi

{"title":"High-Throughput Sequencing Identified Multiple Fig Viruses and Viroids Associated with Fig Mosaic Disease in Iraq.","authors":"Nabeel Al-Kaeath, Shrooq Zagier, Osamah Alisawi, Fadhal Al Fadhal, Naima Mahfoudhi","doi":"10.5423/PPJ.OA.04.2024.0068","DOIUrl":"https://doi.org/10.5423/PPJ.OA.04.2024.0068","url":null,"abstract":"Mosaic is the most common viral disease affecting fig plants. Although the Fig mosaic virus is the leading cause of mosaic disease, other viruses are also involved. High-throughput sequencing was used to assess viral infections in fig plants with mosaic. The genomic DNA and total RNAseq of mosaic-symptomatic fig leaves were sequenced using the Illumina platform. The analysis revealed the presence of fig badnavirus 1 (FBV-1), grapevine badnavirus 1 (GBV-1), citrus exocortis viroid (CEVd), and apple dimple fruit viroid (ADFVd). The FBV-1 and GBV-1 sequences were 7,140 bp and 7,239 bp long, respectively. The two genomes encode one open reading frame containing five major protein domains. The viroids, CEVd and ADFVd, were 397 bp and 305 bp long. Phylogenetic analyses revealed a close relationship between FBV-1 and Iranian isolates of the same species, while GBV-1 was closely related to Russian grapevine badnavirus isolates (Tem64, Blu17, KDH48, and Pal9). CEVd was closely related to other Iraqi isolates, while ADFVd was strongly related to a Spanish isolate. A registered endogenous pararetrovirus, caulimovirus-Fca1, with a size of 7,556 bp, was found in the RNA transcripts with a low expression level. This integrant was also detected in the genomes of the two lines 'Horaishi' (a female line) and 'Caprifig 6085' (a male line). Phylogenetic analyses revealed that caulimovirus-Fca1 was distinct from two other clades of different endogenous virus genera.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"486-497"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Antimicrobial Compounds Produced by Endolichenic Fungi in Different Culture Media. 研究内生真菌在不同培养基中产生的抗菌化合物

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.NT.06.2024.0087

Jaycee Augusto G Paguirigan, Eunah Jeong, Kyo Bin Kang, Jae-Seoun Hur, Wonyong Kim

{"title":"Investigation of Antimicrobial Compounds Produced by Endolichenic Fungi in Different Culture Media.","authors":"Jaycee Augusto G Paguirigan, Eunah Jeong, Kyo Bin Kang, Jae-Seoun Hur, Wonyong Kim","doi":"10.5423/PPJ.NT.06.2024.0087","DOIUrl":"https://doi.org/10.5423/PPJ.NT.06.2024.0087","url":null,"abstract":"Continuous use of synthetic fungicides has led to explosive emergence of fungicide-resistant microbes. Therefore, there are urgent needs for environmentally friendly antimicrobial agents with novel modes of action. This study investigated endolichenic fungi (ELF) as a source of antimicrobial compounds against various plant pathogens. We utilized an One Strain MAny Compounds (OSMAC) approach to enhance the chemical diversity of fourteen ELF. This involved cultivation of ELF in four growth media and subsequently assessing antimicrobial activities of culture extracts. Nearly half of the culture extracts exhibited antimicrobial activity against a Gram-positive bacterium, but showed minimal activity against Gram-negative bacteria tested. Notably, culture extracts from two ELF, Chaetomium globosum and Nodulisporium sp., demonstrated significant inhibitory effects against plant pathogenic fungi. LC-MS/MS-based metabolome profiling confirmed the presence of known bioactive compounds like cyclic dipeptides and chaetoglobosins. These findings highlight the effectiveness of combining OSMAC and metabolomics for identifying antimicrobial agents for agricultural use.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"559-567"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing Cold Plasma's Impact on Banana Growth and Fusarium Wilt Control. 评估冷等离子体对香蕉生长和镰刀菌枯萎病控制的影响

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.05.2024.0083

Priya Rajakumar, Nadiya Akmal Baharum, Afiqah Insyirah Lutfi, Najiah Mohd Sadali, Muhamad Shakirin Mispan, Lim Lian Kuang, Yap Seong Ling, Norzulaani Khalid, Nur Ardiyana Rejab

{"title":"Assessing Cold Plasma's Impact on Banana Growth and Fusarium Wilt Control.","authors":"Priya Rajakumar, Nadiya Akmal Baharum, Afiqah Insyirah Lutfi, Najiah Mohd Sadali, Muhamad Shakirin Mispan, Lim Lian Kuang, Yap Seong Ling, Norzulaani Khalid, Nur Ardiyana Rejab","doi":"10.5423/PPJ.OA.05.2024.0083","DOIUrl":"https://doi.org/10.5423/PPJ.OA.05.2024.0083","url":null,"abstract":"Bananas (Musa spp.), which serve millions of people worldwide, face a serious threat from Fusarium wilt (FW) disease caused by Fusarium oxysporum f. sp. cubense (Foc). Developing disease-resistant varieties particularly through breeding is challenging due to banana's seedless nature (parthenocarpic). As an alternative, cold plasma (CP) technology, has the potential to be used for crop improvement. Our study demonstrates a favourable impact of CP on the growth performance of banana (Berangan cultivar, AAA) in terms of height, leaf number and stem diameter. CP-treated plants also displayed delayed disease progression as well as lower disease severity indicated by slightly lower value of leaf symptoms index and rhizome discoloration index compared to the control plants. Additionally, quantitative real-time polymerase chain reaction analysis revealed differential expression of several defence (PR1, WRKY22, PAL, and CEBiP) and growth (Cytochrome P450, NAC68, and CAT) related genes in CP-treated plants, particularly in conjunction with Foc infection. These findings shed light on the potential use of CP in managing FW in banana and offer insights into possible mechanism behind improved traits.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"463-474"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Tissue-Specific Distribution and Genetic Variation of Alfalfa Mosaic Virus and Chinese Artichoke Mosaic Virus in Chinese Artichoke (Stachys affinis miq.). 紫花苜蓿花叶病毒和中国朝鲜蓟花叶病毒在中国朝鲜蓟（Stachys affinis miq.）

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.06.2024.0094

Ji-Soo Park, Dong-Joo Min, Tae-Seon Park, You-Seop Shin, Jin-Sung Hong

{"title":"Investigation of Tissue-Specific Distribution and Genetic Variation of Alfalfa Mosaic Virus and Chinese Artichoke Mosaic Virus in Chinese Artichoke (Stachys affinis miq.).","authors":"Ji-Soo Park, Dong-Joo Min, Tae-Seon Park, You-Seop Shin, Jin-Sung Hong","doi":"10.5423/PPJ.OA.06.2024.0094","DOIUrl":"10.5423/PPJ.OA.06.2024.0094","url":null,"abstract":"The Chinese artichoke (Stachys affinis syn. S. sieboldii) is a widely cultivated crop, and its rhizome is used as a medicinal vegetable. To investigate the causes of viral diseases in Chinese artichokes, the infection rates of four virus species infecting Chinese artichoke were investigated. Since the Chinese artichoke propagates through its tuber, this study aimed to determine whether viral transmission to the progeny is possible through the tuber, by identifying the virus present in the tuber and investigating its accumulation. First, reverse transcription polymerase chain reaction analysis was performed to detect viruses using total RNA extracted from the flowers, leaves, and tubers of Chinese artichoke plants. Alfalfa mosaic virus (AMV) and Chinese artichoke mosaic virus (ChAMV) had high infectivity in Chinese artichoke and most plants were simultaneously infected with AMV and ChAMV. These viruses were present in all tissues, but their detection frequency and accumulation rates varied across different tissues of the Chinese artichoke. Also, we sequenced the coat protein (CP) genes of AMV and ChAMV to investigate genetic variations of virus between the leaf and tuber. It provides information on CP gene sequences and genetic diversity of isolates identified from new hosts of AMV and ChAMV. This study offers valuable insights into the distribution and spread of the ChAMV and AMV within Chinese artichoke plants, which have implications for the management and control of viral infections in crops.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"390-398"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Metatranscriptomic Analysis of Plant Viruses in Imported Frozen Cherries and Blueberries. 进口冷冻樱桃和蓝莓中植物病毒的元转录组学综合分析。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.06.2024.0088

Ga-Eun Lee, Hyo-Jeong Lee, Rae-Dong Jeong

{"title":"Comprehensive Metatranscriptomic Analysis of Plant Viruses in Imported Frozen Cherries and Blueberries.","authors":"Ga-Eun Lee, Hyo-Jeong Lee, Rae-Dong Jeong","doi":"10.5423/PPJ.OA.06.2024.0088","DOIUrl":"10.5423/PPJ.OA.06.2024.0088","url":null,"abstract":"The possibility of new viruses emerging in various regions worldwide has increased due to a combination of factors, including climate change and the expansion of international trading. Plant viruses spread through various transmission routes, encompassing well-known avenues such as pollen, seeds, and insects. However, research on potential transmission routes beyond these known mechanisms has remained limited. To address this gap, this study employed metatranscriptomic analysis to ascertain the presence of plant viruses in imported frozen fruits, specifically cherries and blueberries. This analysis aimed to identify pathways through which plant viruses may be introduced into countries. Virome analysis revealed the presence of six species of plant viruses in frozen cherries and blueberries: cherry virus A (CVA), prunus necrotic ringspot virus (PNRSV), prune dwarf virus (PDV), prunus virus F (PrVF), blueberry shock virus (BlShV), and blueberry latent virus (BlLV). Identifying these potential transmission routes is crucial for effectively managing and preventing the spread of plant viruses and crop protection. This study highlights the importance of robust quality control measures and monitoring systems for frozen fruits, emphasizing the need for proactive measures to mitigate the risk associated with the potential spread of plant viruses.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"377-389"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potentiality of Beneficial Microbe Bacillus siamensis GP-P8 for the Suppression of Anthracnose Pathogens and Pepper Plant Growth Promotion. 有益微生物暹罗芽孢杆菌 GP-P8 抑制炭疽病病原体和促进辣椒植株生长的潜力。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.01.2024.0022

Ji Min Woo, Hyun Seung Kim, In Kyu Lee, Eun Jeong Byeon, Won Jun Chang, Youn Su Lee

{"title":"Potentiality of Beneficial Microbe Bacillus siamensis GP-P8 for the Suppression of Anthracnose Pathogens and Pepper Plant Growth Promotion.","authors":"Ji Min Woo, Hyun Seung Kim, In Kyu Lee, Eun Jeong Byeon, Won Jun Chang, Youn Su Lee","doi":"10.5423/PPJ.OA.01.2024.0022","DOIUrl":"10.5423/PPJ.OA.01.2024.0022","url":null,"abstract":"This study was carried out to screen the antifungal activity against Colletotrichum acutatum, Colletotrichum dematium, and Colletotrichum coccodes. Bacterial isolate GP-P8 from pepper soil was found to be effective against the tested pathogens with an average inhibition rate of 70.7% in in vitro dual culture assays. 16S rRNA gene sequencing analysis result showed that the effective bacterial isolate as Bacillus siamensis. Biochemical characterization of GP-P8 was also performed. According to the results, protease and cellulose, siderophore production, phosphate solubilization, starch hydrolysis, and indole-3-acetic acid production were shown by the GP-P8. Using specific primers, genes involved in the production of antibiotics, such as iturin, fengycin, difficidin, bacilysin, bacillibactin, surfactin, macrolactin, and bacillaene were also detected in B. siamensis GP-P8. Identification and analysis of volatile organic compounds through solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) revealed that acetoin and 2,3-butanediol were produced by isolate GP-P8. In vivo tests showed that GP-P8 significantly reduced the anthracnose disease caused by C. acutatum, and enhanced the growth of pepper plant. Reverse transcription polymerase chain reaction analysis of pepper fruits revealed that GP-P8 treated pepper plants showed increased expression of immune genes such as CaPR1, CaPR4, CaNPR1, CaMAPK4, CaJA2, and CaERF53. These results strongly suggest that GP-P8 could be a promising biocontrol agent against pepper anthracnose disease and possibly a pepper plant growth-promoting agent.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"346-357"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RT-RPA Assay Combined with a Lateral Flow Strip to Detect Soybean Mosaic Virus. RT-RPA 分析法与侧流带相结合检测大豆花叶病毒。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.02.2024.0027

Bong Geun Oh, Ju-Yeon Yoon, Ho-Jong Ju

{"title":"RT-RPA Assay Combined with a Lateral Flow Strip to Detect Soybean Mosaic Virus.","authors":"Bong Geun Oh, Ju-Yeon Yoon, Ho-Jong Ju","doi":"10.5423/PPJ.OA.02.2024.0027","DOIUrl":"10.5423/PPJ.OA.02.2024.0027","url":null,"abstract":"Soybean (Glycine max L.) is one of the most widely planted and used legumes in the world, being used for food, animal feed products, and industrial production. The soybean mosaic virus (SMV) is the most prevalent virus infecting soybean plants. This study developed a diagnostic method for the rapid and sensitive detection of SMV using a reverse transcription-recombinase polymerase amplification (RT-RPA) technique combined with a lateral flow strip (LFS). The RT-RPA and RT-RPA-LFS conditions to detect the SMV were optimized using the selected primer set that amplified part of the VPg protein gene. The optimized reaction temperature for the RT-RPA primer and RT-RPA-LFS primer used in this study was 38℃ for both, and the minimum reaction time was 10 min and 5 min, respectively. The RT-RPA-LFS was as sensitive as RT-PCR to detect SMV with 10 pg/μl of total RNA. The reliability of the developed RT-RPA-LFS assay was evaluated using leaves collected from soybean fields. The RT-RPA-LFS diagnostic method developed in this study will be useful as a diagnostic method that can quickly and precisely detect SMV in the epidemiological investigation of SMV, in the selection process of SMV-resistant varieties, on local farms with limited resources.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"337-345"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light- and Relative Humidity-Regulated Hypersensitive Cell Death and Plant Immunity in Chinese Cabbage Leaves by a Non-adapted Bacteria Xanthomonas campestris pv. vesicatoria. 非适应细菌野油菜黄单胞菌 pv. vesicatoria 对光照和相对湿度调控的大白菜叶片超敏细胞死亡和植物免疫作用

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.03.2024.0057

Young Hee Lee, Yun-Hee Kim, Jeum Kyu Hong

{"title":"Light- and Relative Humidity-Regulated Hypersensitive Cell Death and Plant Immunity in Chinese Cabbage Leaves by a Non-adapted Bacteria Xanthomonas campestris pv. vesicatoria.","authors":"Young Hee Lee, Yun-Hee Kim, Jeum Kyu Hong","doi":"10.5423/PPJ.OA.03.2024.0057","DOIUrl":"10.5423/PPJ.OA.03.2024.0057","url":null,"abstract":"Inoculation of Chinese cabbage leaves with high titer (107 cfu/ml) of the non-adapted bacteria Xanthomonas campestris pv. vesicatoria (Xcv) strain Bv5-4a.1 triggered rapid leaf tissue collapses and hypersensitive cell death (HCD) at 24 h. Electrolyte leakage and lipid peroxidation markedly increased in the Xcv-inoculated leaves. Defence-related gene expressions (BrPR1, BrPR4, BrChi1, BrGST1 and BrAPX1) were preferentially activated in the Xcv-inoculated leaves. The Xcv-triggered HCD was attenuated by continuous light but accelerated by a dark environment, and the prolonged high relative humidity also alleviated the HCD. Constant dark and increased relative humidity provided favorable conditions for the Xcv bacterial growth in the leaves. Pretreated fluridone (biosynthetic inhibitor of endogenous abscisic acid [ABA]) increased the HCD in the Xcv-inoculated leaves, but exogenous ABA attenuated the HCD. The pretreated ABA also reduced the Xcv bacterial growth in the leaves. These results highlight that the onset of HCD in Chinese cabbage leaves initiated by non-adapted pathogen Xcv Bv5-4a.1 and in planta bacterial growth was differently modulated by internal and external conditional changes.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"358-376"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Application of Reverse Transcription Nanoplate-Based Digital PCR Assay for Sensitive and Accurate Detection of Rice Black-Streaked Dwarf Virus in Cereal Crops. 基于反转录纳米片的数字 PCR 检测方法的开发与应用，用于灵敏、准确地检测谷类作物中的水稻黑条矮缩病病毒。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.NT.03.2024.0048

Hyo-Jeong Lee, Hae-Jun Kim, Sang-Min Kim, Rae-Dong Jeong

{"title":"Development and Application of Reverse Transcription Nanoplate-Based Digital PCR Assay for Sensitive and Accurate Detection of Rice Black-Streaked Dwarf Virus in Cereal Crops.","authors":"Hyo-Jeong Lee, Hae-Jun Kim, Sang-Min Kim, Rae-Dong Jeong","doi":"10.5423/PPJ.NT.03.2024.0048","DOIUrl":"10.5423/PPJ.NT.03.2024.0048","url":null,"abstract":"The emergence of rice black-streaked dwarf virus (RBSDV) poses a significant threat to global cereal crop cultivation, necessitating the urgent development of reliable detection and quantification techniques. This study introduces a reliable approach for the precise and sensitive quantification of the RBSDV in cereal crop samples, employing a reverse transcription digital polymerase chain reaction (RT-dPCR) assay. We assessed the specificity and sensitivity of the RT-dPCR assay proposed for precise RBSDV detection and quantification. Our findings demonstrate that RT-dPCR was specific for detection of RBSDV, with no cross-reactivity observed with other viruses infecting cereal crops. The RT-dPCR sensitivity was over 10 times that of RT-quantitative PCR (RT-qPCR). The detection limit of RT-dPCR was 0.096 copies/μl. In addition, evaluation of RT-dPCR assay with field samples was conducted on 60 different cereal crop samples revealed that RT-dPCR (45/60) exhibited superior accuracy compared with RT-qPCR (23/60). In this study, we present a specific and accurate RT-dPCR assay for the detection and quantification of RBSDV.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"408-413"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia. 导致印度尼西亚芒果枯萎病的 Colletotrichum spp.

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.NT.04.2024.0061

Khaerani Nurlaelita, Arif Wibowo, Ani Widiastuti

{"title":"Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia.","authors":"Khaerani Nurlaelita, Arif Wibowo, Ani Widiastuti","doi":"10.5423/PPJ.NT.04.2024.0061","DOIUrl":"10.5423/PPJ.NT.04.2024.0061","url":null,"abstract":"Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch's Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"399-407"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0