Plant Pathology Journal最新文献_第9页

Development of the Droplet Digital PCR Method for the Detection and Quantification of Erwinia pyrifoliae. 液滴数字PCR检测与定量方法的建立。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.NT.08.2022.0117

Lin He, Seong Hwan Kim, Jun Myoung Yu

{"title":"Development of the Droplet Digital PCR Method for the Detection and Quantification of Erwinia pyrifoliae.","authors":"Lin He, Seong Hwan Kim, Jun Myoung Yu","doi":"10.5423/PPJ.NT.08.2022.0117","DOIUrl":"https://doi.org/10.5423/PPJ.NT.08.2022.0117","url":null,"abstract":"Black shoot blight disease caused by Erwinia pyrifoliae has serious impacts on quality and yield in pear production in Korea; therefore, rapid and accurate methods for its detection are needed. However, traditional detection methods require a great deal of time and fail to achieve absolute quantification. In the present study, we developed a droplet digital polymerase chain reaction (ddPCR) method for the detection and absolute quantification of E. pyrifoliae using a pair of species-specific primers. The detection range was 103 - 107 copies/ml (DNA templates) and cfu/ml (cell culture templates). This new method exhibited good linearity and repeatability and was validated by absolute quantification of E. pyrifoliae DNA copies from samples of artificially inoculated immature pear fruits. Here, we present the first study of ddPCR assay for the detection and quantification of E. pyrifoliae. This method has potential applications in epidemiology and for the early prediction of black shoot blight outbreaks.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"141-148"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ad/ed/ppj-nt-08-2022-0117.PMC9929165.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10737273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Oomycete Activity and Pepper Root Colonization of Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 against Phytophthora capsici. 紫舌假单胞菌YJR13和腐臭假单胞菌YJR92抗辣椒疫霉菌活性及辣椒根定殖

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.01.2023.0001

Elena Volynchikova, Ki Deok Kim

{"title":"Anti-Oomycete Activity and Pepper Root Colonization of Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 against Phytophthora capsici.","authors":"Elena Volynchikova, Ki Deok Kim","doi":"10.5423/PPJ.OA.01.2023.0001","DOIUrl":"https://doi.org/10.5423/PPJ.OA.01.2023.0001","url":null,"abstract":"Previously, Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 from a sequential screening procedure were proven to effectively control Phytophthora blight caused by Phytophthora capsici. In this study, we further investigated the anti-oomycete activities of these strains against mycelial growth, zoospore germination, and germ tube elongation of P. capsici. We also investigated root colonization ability of the bacterial strains in square dishes, including cell motility (swimming and swarming motilities) and biofilm formation. Both strains significantly inhibited mycelial growth in liquid and solid V8 juice media and M9 minimal media, zoospore germination, and germ tube elongation compared with Bacillus vallismortis EXTN-1 (positive biocontrol strain), Sphingomonas aquatilis KU408 (negative biocontrol strain), and MgSO4 solution (untreated control). In diluted (nutrient-deficient) V8 juice broth, the tested strain populations were maintained at >108 cells/ml, simultaneously providing mycelial inhibitory activity. Additionally, these strains colonized pepper roots at a 106 cells/ml concentration for 7 days. The root colonization of the strains was supported by strong swimming and swarming activities, biofilm formation, and chemotactic activity towards exudate components (amino acids, organic acids, and sugars) of pepper roots. Collectively, these results suggest that strains YJR13 and YJR92 can effectively suppress Phytophthora blight of pepper through direct anti-oomycete activities against mycelial growth, zoospore germination and germ tube elongation. Bacterial colonization of pepper roots may be mediated by cell motility and biofilm formation together with chemotaxis to root exudates.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"123-135"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/17/45/ppj-oa-01-2023-0001.PMC9929162.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10737267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Detection of Phytoplasmas of the 16SrⅠ and 16SrXXXⅡ Groups in Elaeocarpus sylvestris Trees with Decline Disease in Jeju Island, South Korea. 济州岛Elaeocarpus sylvestris病树16SrⅠ和16SrXXXⅡ群植物原体的分子检测

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.07.2022.0091

Geon-Woo Lee, Sang-Sub Han

{"title":"Molecular Detection of Phytoplasmas of the 16SrⅠ and 16SrXXXⅡ Groups in Elaeocarpus sylvestris Trees with Decline Disease in Jeju Island, South Korea.","authors":"Geon-Woo Lee, Sang-Sub Han","doi":"10.5423/PPJ.OA.07.2022.0091","DOIUrl":"https://doi.org/10.5423/PPJ.OA.07.2022.0091","url":null,"abstract":"Phytoplasmas were discovered in diseased Elaeocarpus sylvestris trees growing on Jeju Island that showed symptoms of yellowing and darkening in the leaves. Leaf samples from 14 symptomatic plants in Jeju-si and Seogwipo-si were collected and phytoplasma 16S rRNA was successfully amplified by nested polymerase chain reaction using universal primers. The sequence analysis detected two phytoplasmas, which showed 99.5% identity to 'Candidatus Phytoplasma asteris' and 'Ca. P. malaysianum' affiliated to 16SrI and 16SrXXXII groups, respectively. Through polymerase chain reaction-restriction fragment length polymorphism (RFLP) analyses using the AfaI (RsaI) restriction enzyme, the presence of two phytoplasmas strains as well as cases of mixed infection of these strains was detected. In a virtual RFLP analysis with 17 restriction enzymes, the 16S rRNA sequence of the 'Ca. P. asteris' strain was found to match the pattern of the 16SrI-B subgroup. In addition, the phytoplasmas in the mixed-infection cases could be distinguished using specific primer sets. In conclusion, this study confirmed mixed infection of two phytoplasmas in one E. sylvestris plant, and also the presence of two phytoplasmas (of the 16SrⅠ and 16SrXXXⅡ groups) in Jeju Island (Republic of Korea).","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"149-157"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/45/54/ppj-oa-07-2022-0091.PMC9929163.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10737268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outbreak of Rice Panicle Blast in Jeonbuk Province of Korea in 2021. 2021年全北爆发水稻穗瘟。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.NT.07.2022.0103

Hyunjung Chung, Woo-Il Lee, Soo Yeon Choi, Nak-Jung Choi, Sang-Min Kim, Ju-Yeon Yoon, Bong Choon Lee

引用次数: 0

Copper-Based Compounds against Erwinia amylovora: Response Parameter Analysis and Suppression of Fire Blight in Apple. 铜基化合物对苹果纹枯病的防治效果及参数分析。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.07.2022.0100

Duck Kyu Ryu, Mahesh Adhikari, Dong Hyuk Choi, Kyung Jin Jun, Do Hyoung Kim, Chae Ryeong Kim, Min Kyu Kang, Duck Hwan Park

{"title":"Copper-Based Compounds against Erwinia amylovora: Response Parameter Analysis and Suppression of Fire Blight in Apple.","authors":"Duck Kyu Ryu, Mahesh Adhikari, Dong Hyuk Choi, Kyung Jin Jun, Do Hyoung Kim, Chae Ryeong Kim, Min Kyu Kang, Duck Hwan Park","doi":"10.5423/PPJ.OA.07.2022.0100","DOIUrl":"https://doi.org/10.5423/PPJ.OA.07.2022.0100","url":null,"abstract":"Fire blight, caused by Erwinia amylovora, is one of the major bacterial disease of apple and pear, causing enormous economic losses worldwide. Several control measures against E. amylovora have been reported till date, however, none of them have proved to be effective significantly against the pathogen. In this study, mechanisms of the copper-based control agents (CBCAs): copper oxychloride (COCHL), copper oxide (COX), copper hydroxide (CHY), copper sulfate basic (CSB), and tribasic copper sulfate (TCS) and their disease severity reduction efficacy against E. amylovora were analyzed. Bis-1,3-dibutylbarbituric acid trimethine oxonol, carboxyl fluorescein diacetate succinimidyl ester, and 5-cyano-2,3-ditolyl tetrazolium chloride staining were used to check the damage of membrane potential, cytoplasmic pHin, and respiration of CBCAs-treated E. amylovora, respectively. High disturbance in the membrane potential of E. amylovora was found under COX and COCHL treatments. Similarly, higher significant changes in the inner cytoplasmic pHin were observed under COX, COCHL, and TCS treatment. CHY and COCHL-treated E. amylovora showed a significant reduction in respiration. In vitro bioassay results revealed that CHY, CSB, and TCS at 2,000 ppm reduced the severity of fire blight both in pre- and post-treatment of CBCAs in immature apple fruits and seedlings. Overall, the most effective CBCAs against E. amylovora could be CHY at 2,000 ppm as its showed inhibition mechanisms and disease severity reduction.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"52-61"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/40/7b/ppj-oa-07-2022-0100.PMC9929174.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10792971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparative Bioactivity of Emamectin Benzoate Formulations against the Pine Wood Nematode, Bursaphelenchus xylophilus. 苯甲酸埃维菌素制剂对松材线虫生物活性的比较研究。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.08.2022.0120

Jong-Won Lee, Abraham Okki Mwamula, Jae-Hyuk Choi, Ho-Wook Lee, Yi Seul Kim, Jin-Hyo Kim, Yong-Hwa Choi, Dong Woon Lee

{"title":"Comparative Bioactivity of Emamectin Benzoate Formulations against the Pine Wood Nematode, Bursaphelenchus xylophilus.","authors":"Jong-Won Lee, Abraham Okki Mwamula, Jae-Hyuk Choi, Ho-Wook Lee, Yi Seul Kim, Jin-Hyo Kim, Yong-Hwa Choi, Dong Woon Lee","doi":"10.5423/PPJ.OA.08.2022.0120","DOIUrl":"https://doi.org/10.5423/PPJ.OA.08.2022.0120","url":null,"abstract":"The pine wood nematode (PWN), Bursaphelenchus xylophilus is a well-known devastating pathogen of economic importance in the Republic of Korea and other countries. In the Republic of Korea, trunk injection of nematicides is the preferred method of control. In this study, the efficacy of 16 locally produced formulations of emamectin benzoate against the PWN are compared through determining their sublethal toxicities and reproduction inhibition potentials. Nematodes were treated with varying concentrations of the tested chemicals in multi-well culture plates, and rates of paralysis and mortality were determined after 24 h. Reproduction inhibition potential was tested by inoculating pre-treated nematodes onto Botrytis cinerea, and in pine twig cuttings. Despite the uniformity in the concentration of the active ingredient, efficacy was contrastingly different among formulations. The formulations evidently conformed to three distinct groups based on similarities in sublethal activity (group 1: LC95 of 0.00768-0.01443 mg/ml; group 2: LC95 of 0.03202-0.07236 mg/ml, and group 3: LC95 of as high as 0.30643-0.40811 mg/ml). Nematode paralysis generally occurred at the application dose of 0.0134-0.1075 μg/ml, and there were significant differences in nematode paralysis rates among the products. Nematode reproduction was only evident at lower doses both on B. cinerea and pine twigs, albeit the variations among formulations. Group 1 formulations significantly reduced nematode reproduction even at a lower dose of 0.001075 µg/ml. The variations in efficacy might be attributed to differences in inert ingredients. Therefore, there is need to analyze the potential antagonistic effects of the large number of additives used in formulations.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"75-87"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5b/e1/ppj-oa-08-2022-0120.PMC9929175.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10737264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana. 土壤链霉菌对番茄和香蕉枯萎病的生物防治及促生效果评价。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.08.2022.0124

Praphat Kawicha, Jariya Nitayaros, Prakob Saman, Sirikanya Thaporn, Thanwanit Thanyasiriwat, Khanitta Somtrakoon, Kusavadee Sangdee, Aphidech Sangdee

{"title":"Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana.","authors":"Praphat Kawicha, Jariya Nitayaros, Prakob Saman, Sirikanya Thaporn, Thanwanit Thanyasiriwat, Khanitta Somtrakoon, Kusavadee Sangdee, Aphidech Sangdee","doi":"10.5423/PPJ.OA.08.2022.0124","DOIUrl":"https://doi.org/10.5423/PPJ.OA.08.2022.0124","url":null,"abstract":"Fusarium oxysporum f. sp. lycopersici (Fol) and Fusarium oxysporum f. sp. cubense (Foc), are the causal agent of Fusarium wilt disease of tomato and banana, respectively, and cause significant yield losses worldwide. A cost-effective measure, such as biological control agents, was used as an alternative method to control these pathogens. Therefore, in this study, six isolates of the Streptomyces-like colony were isolated from soils and their antagonistic activity against phytopathogenic fungi and plant growth-promoting (PGP) activity were assessed. The results showed that these isolates could inhibit the mycelial growth of Fol and Foc. Among them, isolate STRM304 showed the highest percentage of mycelial growth reduction and broad-spectrum antagonistic activity against all tested fungi. In the pot experiment study, the culture filtrate of isolates STRM103 and STRM104 significantly decreased disease severity and symptoms in Fol inoculated plants. Similarly, the culture filtrate of the STRM304 isolate significantly reduced the severity of the disease and symptoms of the disease in Foc inoculated plants. The PGP activity test presents PGP activities, such as indole acetic acid production, phosphate solubilization, starch hydrolysis, lignin hydrolysis, and cellulase activity. Interestingly, the application of the culture filtrate from all isolates increased the percentage of tomato seed germination and stimulated the growth of tomato plants and banana seedlings, increasing the elongation of the shoot and the root and shoot and root weight compared to the control treatment. Therefore, the isolate STRM103 and STRM104, and STRM304 could be used as biocontrol and PGP agents for tomato and banana, respectively, in sustainable agriculture.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"108-122"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/48/df/ppj-oa-08-2022-0124.PMC9929171.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10737265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Identification and Characterization of Pseudomonas syringae pv. syringae, a Causative Bacterium of Apple Canker in Korea. 丁香假单胞菌的鉴定与鉴定。苹果溃疡病的一种致病细菌——丁香菌。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-02-01 DOI: 10.5423/PPJ.OA.08.2022.0121

Seunghee Lee, Wonsu Cheon, Hyeok Tae Kwon, Younmi Lee, Jungyeon Kim, Kotnala Balaraju, Yongho Jeon

{"title":"Identification and Characterization of Pseudomonas syringae pv. syringae, a Causative Bacterium of Apple Canker in Korea.","authors":"Seunghee Lee, Wonsu Cheon, Hyeok Tae Kwon, Younmi Lee, Jungyeon Kim, Kotnala Balaraju, Yongho Jeon","doi":"10.5423/PPJ.OA.08.2022.0121","DOIUrl":"https://doi.org/10.5423/PPJ.OA.08.2022.0121","url":null,"abstract":"In the present investigation, bacterial isolates from infected apple trees causing apple canker during winter were studied in the northern Gyeongbuk Province, Korea. The pathogen was identified as Pseudomonas syringae pv. syringae (Pss) through various physiological and biochemical characterization assays such as BIOLOG, gas chromatography of fatty acid methyl esters, and 16S rRNA. Bioassays for the production of phytotoxins were positive for syringopeptin and syringomycin against Bacillus megaterium and Geotrichum candidum, respectively. The polymerase chain reaction (PCR) method enabled the detection of toxin-producing genes, syrB1, and sypB in Pss. The differentiation of strains was performed using LOPAT and GATTa tests. Pss further exhibited ice nucleation activity (INA) at a temperature of -0.7°C, indicating an INA+ bacterium. The ice-nucleating temperature was -4.7°C for a non-treated control (sterilized distilled water), whereas it was -9.6°C for an INA- bacterium Escherichia coli TOP10. These methods detected pathogenic strains from apple orchards. Pss might exist in an apple tree during ice injury, and it secretes a toxin that makes leaves yellow and cause canker symptoms. Until now, Korea has not developed antibiotics targeting Pss. Therefore, it is necessary to develop effective disease control to combat Pss in apple orchards. Pathogenicity test on apple leaves and stems showed canker symptoms. The pathogenic bacterium was re-isolated from symptomatic plant tissue and confirmed as original isolates by 16S rRNA. Repetitive element sequence-based PCR and enterobacterial repetitive intergenic consensus PCR primers revealed different genetic profiles within P. syringae pathovars. High antibiotic susceptibility results showed the misreading of mRNA caused by streptomycin and oxytetracycline.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 1","pages":"88-107"},"PeriodicalIF":2.3,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/79/61/ppj-oa-08-2022-0121.PMC9929173.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10792977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Morphometric Analysis of Isolated Conidia of Various Species of Colletotrichum sp. from Avocado and Mango in Côte d'Ivoire Côte科特迪瓦鳄梨和芒果炭疽菌属分离分生孢子形态计量学分析

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-01-15 DOI: 10.3923/ppj.2023.1.16

Adjoa Kouadia, E. Tiénébo, K. Kouadio, N. Adja, K. Abo

引用次数: 0

Evidence for Upholding the Association of Whitefly Species Bemisia tabaci and Trialeurodes vaporariorum with Tomato Yellow Leaf Curl Virus 支持烟粉虱和白粉虱与番茄黄曲叶病毒关联的证据

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-01-15 DOI: 10.3923/ppj.2023.25.38

R. ..., Rakesh Kumar, S. Kapoor, A. Handa

引用次数: 0