Plant Pathology Journal最新文献_第8页

Long-term Impact of Virus-Free Apple Seedlings on Fruit Quality and Yield in Commercial Orchards of Korea. 苹果脱毒苗对韩国商品果园果实品质和产量的长期影响

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.NT.09.2025.0135

Sang-Yun Cho, Hyun Ran Kim, Se Hee Kim, Byeonghyeon Yun, Sewon Oh

引用次数: 0

Antagonistic Activity and Genomic Insights of Bacillus velezensis JE250 against Erwinia amylovora. 韦氏芽孢杆菌JE250对淀粉杆菌的拮抗活性及基因组学研究。

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.OA.03.2025.0037

Jueun Lee, Won-Kwon Jung, Hee-Young Jung, Yong Chull Jeun, Yongho Jeon, Hyong Woo Choi

{"title":"Antagonistic Activity and Genomic Insights of Bacillus velezensis JE250 against Erwinia amylovora.","authors":"Jueun Lee, Won-Kwon Jung, Hee-Young Jung, Yong Chull Jeun, Yongho Jeon, Hyong Woo Choi","doi":"10.5423/PPJ.OA.03.2025.0037","DOIUrl":"10.5423/PPJ.OA.03.2025.0037","url":null,"abstract":"Fire blight, caused by Erwinia amylovora, is a highly destructive bacterial disease that affects apple and pear orchards worldwide, leading to significant economic losses. In this study, we isolated and characterized endophytic bacterial strains from apple trees in Gyeongsangbuk-do, South Korea, to identify potential biocontrol agents against E. amylovora. Among the five antagonistic strains identified, Bacillus velezensis JE80 and JE250 exhibited the strongest inhibitory effects. Further analysis using culture filtrates (CFs) from these strains demonstrated that the CFs of JE80 and JE250 not only suppressed E. amylovora growth in a growth-phase-dependent manner but also significantly impaired bacterial motility and biofilm formation. Notably, in planta assays revealed that JE250 effectively reduced fire blight symptoms in apple blossoms, performing comparably to streptomycin sulfate. Whole-genome sequencing of JE250 identified biosynthetic gene clusters associated with the production of antimicrobial compounds, including difficidin, fengycin, bacillaene, macrolactin, bacillibactin, and bacilysin, further supporting its strong antagonistic potential. These findings suggest that B. velezensis JE250 is a promising biocontrol agent for sustainable fire blight management. Future research should focus on optimizing formulation methods for field application, characterizing specific antimicrobial compounds, and evaluating its long-term efficacy in orchard environments.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 6","pages":"723-735"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Association Study Using High-Density SNP Chip Markers Revealed Novel Bacterial Panicle Blight (Burkholderia glumae) Resistance Genes in Rice. 利用高密度SNP芯片标记的全基因组关联研究揭示了水稻抗穗枯病的新基因。

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.OA.07.2025.0089

Seong-Gyu Jang, Sumin Jo, Sais-Beul Lee, Ji-Min Kim, Nkulu Rolly Kabange, Duyoung Lee, So-Yeon Park, Haeun Choi, Ju-Won Kang, So-Myeong Lee, Youngho Kwon, Jisu Choi, Jong-Min Jeong, Young-Su Seo, Soon-Wook Kwon, Jong-Hee Lee, Dong-Soo Park

{"title":"Genome-Wide Association Study Using High-Density SNP Chip Markers Revealed Novel Bacterial Panicle Blight (Burkholderia glumae) Resistance Genes in Rice.","authors":"Seong-Gyu Jang, Sumin Jo, Sais-Beul Lee, Ji-Min Kim, Nkulu Rolly Kabange, Duyoung Lee, So-Yeon Park, Haeun Choi, Ju-Won Kang, So-Myeong Lee, Youngho Kwon, Jisu Choi, Jong-Min Jeong, Young-Su Seo, Soon-Wook Kwon, Jong-Hee Lee, Dong-Soo Park","doi":"10.5423/PPJ.OA.07.2025.0089","DOIUrl":"10.5423/PPJ.OA.07.2025.0089","url":null,"abstract":"Bacterial panicle blight (BPB) is a serious rice disease that causes spikelet abortion and yield loss under high-temperature and humid conditions. To identify the genetic basis of BPB resistance, we performed a genomewide association study (GWAS) using 307 Korean rice cultivars. A significant quantitative trait locus (QTL), qBG6.1, was identified on chromosome 6, with a lead single nucleotide polymorphism surpassing the genome-wide significance threshold. Within this QTL, haplotype analysis based on whole-genome resequencing data from 157 accessions revealed two candidate genes significantly associated with the percentage of healthy seeds per panicle: Os06g0255900 and Os06g0259850. These genes encode proteins with functions related to exocyst-mediated vesicle trafficking (EXO70F5) and pathogen response (tobacco mosaic virus [TMV]-related protein), respectively, indicating their potential involvement in BPB resistance mechanisms. Our findings highlight the value of integrating GWAS and haplotype analysis to dissect complex traits such as disease resistance. Although the functional roles of these candidate genes require further validation, they represent promising targets for molecular breeding and future genetic studies aimed at developing BPB-resistant rice cultivars.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 6","pages":"780-789"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Planta Application and Efficacy of FireFighter-A Phage Cocktail for Combating Fire Blight. Fire - a噬菌体鸡尾酒在植物上的应用及防治火疫病的效果。

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.OA.06.2025.0079

Sang Guen Kim, Byeori Kim, Sujin Song, Su Jin Jo, Seung Yeup Lee, Se Chang Park, Eunjung Roh

{"title":"In Planta Application and Efficacy of FireFighter-A Phage Cocktail for Combating Fire Blight.","authors":"Sang Guen Kim, Byeori Kim, Sujin Song, Su Jin Jo, Seung Yeup Lee, Se Chang Park, Eunjung Roh","doi":"10.5423/PPJ.OA.06.2025.0079","DOIUrl":"10.5423/PPJ.OA.06.2025.0079","url":null,"abstract":"Fire blight, caused by Erwinia amylovora, poses a significant threat to Rosaceae crops and has caused substantial damage to South Korea since its emergence in 2015. Traditional control methods, including antibiotic- and copper-based treatments, have shown limitations, underscoring the necessity for alternative solutions to enhance efficacy while addressing the concerns associated with antibiotics. This study evaluated the efficacy of FireFighter-A, a novel phage cocktail, in managing fire blight. Comprising four phages-Fifi318, Fifi451, pEa_27, and pEa_47-FireFighter-A demonstrates a broad host range that covers all recent isolates against E. amylovora and E. pyrifoliae, along with high stability under various conditions, including temperature, pH, and buffers. Trials with immature apple fruits, tissue culture rootstock plantlets, acclimated M26 rootstock plantlets, and blossoms have demonstrated that FireFighter-A provides superior biocontrol efficacy through synergistic effects, outperforming individual phages. Moreover, it significantly reduced fire blight symptoms and infection rates with a performance comparable to or exceeding that of streptomycin. These findings support the use of FireFighter-A as an effective and environment-friendly alternative for fire blight control.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 6","pages":"843-855"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Fusarium fujikuroi Species Complex in Korea: Taxonomic Revision, New Records, and Description of Fusarium ipomoeicola sp. nov. 韩国fujikuroi镰刀菌种群：分类修订、新记录和描述。

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.OA.08.2025.0114

Le Dinh Thao, Yunhee Choi, Jung-Wook Yang, Neriman Yilmaz, Jae Sung Lee, Anbazhagan Mageswari, Daseul Lee, Donghun Kang, Frederick Leo Sossah, In-Young Choi, Seung-Beom Hong

{"title":"The Fusarium fujikuroi Species Complex in Korea: Taxonomic Revision, New Records, and Description of Fusarium ipomoeicola sp. nov.","authors":"Le Dinh Thao, Yunhee Choi, Jung-Wook Yang, Neriman Yilmaz, Jae Sung Lee, Anbazhagan Mageswari, Daseul Lee, Donghun Kang, Frederick Leo Sossah, In-Young Choi, Seung-Beom Hong","doi":"10.5423/PPJ.OA.08.2025.0114","DOIUrl":"10.5423/PPJ.OA.08.2025.0114","url":null,"abstract":"The Fusarium fujikuroi species complex (FFSC) includes numerous phytopathogenic and mycotoxigenic species of significant agricultural importance. In this study, 81 Korean isolates within the FFSC from the Korean Agricultural Culture Collection (KACC) were re-identified using multi-locus sequence analyses of partial gene fragments of the translation elongation factor 1-alpha (tef1), beta-tubulin (tub2), calmodulin (CaM), RNA polymerase II largest subunit (rpb1), and RNA polymerase II second largest subunit (rpb2). Phylogenetic analyses clarified the taxonomic identities of these isolates, revealing that many strains previously reported as F. proliferatum, F. subglutinans, and F. circinatum were re-identified as F. annulatum, F. dendrobii, and a novel species, F. ipomoeicola sp. nov., respectively. In total, eight species were confirmed within the FFSC, including seven known species (F. annulatum, F. concentricum, F. dendrobii, F. elaeagni, F. fujikuroi, F. planum, and F. thapsinum) and one novel species described herein. Notably, the taxonomic status of four recently described species was revised, indicating that F. hipposidericola, F. jacksoniae, F. xishuangbannaense, and F. oryzigenum are synonyms of F. annulatum, F. babinda, F. hechiense, and F. planum, respectively. In addition, F. annulatum, F. dendrobii, F. elaeagni, and F. planum are reported for the first time in Korea, and 22 previously undocumented fungus-host associations from Korea were identified, including 15 novel combinations not previously reported worldwide. However, the pathogenicity of these fungal species on their respective hosts was not confirmed in this study.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 6","pages":"820-842"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenerational Dynamics of Endophytic Microbiome in Soybean Seeds. 大豆种子内生微生物群的代际动态。

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.OA.07.2025.0087

Jiwon Kim, Seonwoo Choi, Hyun Kim, Mehwish Roy, Seongho Ahn, Ji Sun Yang, Ho Won Jung, Junhyun Jeon

{"title":"Transgenerational Dynamics of Endophytic Microbiome in Soybean Seeds.","authors":"Jiwon Kim, Seonwoo Choi, Hyun Kim, Mehwish Roy, Seongho Ahn, Ji Sun Yang, Ho Won Jung, Junhyun Jeon","doi":"10.5423/PPJ.OA.07.2025.0087","DOIUrl":"10.5423/PPJ.OA.07.2025.0087","url":null,"abstract":"Seeds harbor diverse microbial communities, including endophytes, some of which are vertically transmitted and may contribute to plant health and productivity. However, the temporal dynamics of seed endophytic communities remain poorly understood in many crop species. In this study, we monitored the composition of bacterial and fungal endophytes in soybean (Glycine max) seeds, along with rhizosphere microbiomes, across three plant generations using a culture-independent approach. Our results revealed two key patterns: seed endophytic communities are distinct from those of bulk soil and rhizosphere microbiomes; and the composition of seed endophytes fluctuates over generations, likely influenced by both pre-existing endophytes, environmental factors, and microbial influx from the surrounding soil and rhizosphere, suggesting possible microbial transmission from the rhizosphere into seeds. Interestingly, despite generational variation, the seed fungal endophyte communities consistently maintained higher phylogenetic diversity compared to bacterial endophytes, which showed limited overlap across generations and were composed of fewer, closely related taxa. Analysis of community assembly mechanisms indicated that both seed and rhizosphere microbiomes significantly contributed to the next generation of seed microbiota, primarily through stochastic drift and homogeneous selection processes. Collectively, our findings offer valuable insights into the intergenerational dynamics of seed endophytes in soybean and provide a foundation for future efforts to harness seed-associated microbiomes for improving crop health and productivity.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 6","pages":"856-867"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptomyces sp. JCK-7385 Effectively Controls Fusarium Head Blight in Rice and Wheat. 链霉菌JCK-7385有效防治稻麦赤霉病。

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.OA.09.2025.0126

Sy Bien Vuong, Ae Ran Park, Yu Jeong Yeo, Loan Thi Thanh Nguyen, Hang Ha Le, Feng Luo, Jin-Cheol Kim

{"title":"Streptomyces sp. JCK-7385 Effectively Controls Fusarium Head Blight in Rice and Wheat.","authors":"Sy Bien Vuong, Ae Ran Park, Yu Jeong Yeo, Loan Thi Thanh Nguyen, Hang Ha Le, Feng Luo, Jin-Cheol Kim","doi":"10.5423/PPJ.OA.09.2025.0126","DOIUrl":"10.5423/PPJ.OA.09.2025.0126","url":null,"abstract":"Fusarium head blight (FHB) primarily caused by Fusarium species including F. graminearum and F. asiaticum, is a major fungal disease affecting wheat, rice, and other cereal crops worldwide. Chemical fungicides remain the primary means of FHB management owing to their convenience and efficacy. Nonetheless, the overuse of chemical pesticides has led to fungicide resistance, human health effects, and environmental contamination, driving a shift toward biocontrol agents as sustainable alternatives for managing plant pathogens. This study aims to evaluate Streptomyces sp. JCK-7385, a rice-associated isolate, is an environmentally friendly biocontrol agent against FHB. The strain produced indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylic acid deaminase, as well as extracellular enzymes including cellulase, gelatinase, and chitinase. JCK-7385 treatments induced defense-related marker gene PR1 expression in transgenic Arabidopsis, as confirmed via β-glucuronidase assays, suggesting an induced resistance mechanism. In greenhouse trials, the JCK-7385 cell suspension and its suspension concentrate formulation (JCK-7385 SC) at a 2,000-fold concentration effectively reduced FHB incidence in rice by 42.3% and 52.5%, respectively. JCK-7385 SC also maintained high efficacy (54.4% control value) after storage at 45°C for 6 weeks. Furthermore, the integrated treatment of JCK-7385 SC and the chemical fungicide (Peulrei) significantly enhanced FHB control compared to single treatments, achieving 63.3% and 71.7% efficacy in rice and wheat, respectively. Field trials demonstrated that this collaborative treatment effectively suppressed FHB development in rice by 52.0%, outperforming individual applications. These findings suggest that Streptomyces sp. JCK-7385 has strong potential as a biological fungicide for FHB management through an induced resistance mechanism.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 6","pages":"800-819"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contamination-Free Genome Assembly of Podosphaera xanthii Reveals Signatures of Obligate Biotrophy. 黄氏水蛭无公害基因组组装揭示专性生物营养特征。

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.NT.07.2025.0098

Nihar Sahu, Yoeguang Hue, Yebin Nam, Hyun-Sook Lee, Hoy-Taek Kim, Masao Watanabe, Ki-Tae Kim, Jong-In Park

{"title":"Contamination-Free Genome Assembly of Podosphaera xanthii Reveals Signatures of Obligate Biotrophy.","authors":"Nihar Sahu, Yoeguang Hue, Yebin Nam, Hyun-Sook Lee, Hoy-Taek Kim, Masao Watanabe, Ki-Tae Kim, Jong-In Park","doi":"10.5423/PPJ.NT.07.2025.0098","DOIUrl":"10.5423/PPJ.NT.07.2025.0098","url":null,"abstract":"Podosphaera xanthii is an obligate biotrophic fungus responsible for powdery mildew disease in cucurbits. Its strict host dependency has hindered genomic studies due to challenges in isolating pure fungal DNA. In this study, we present a high-quality genome assembly of P. xanthii Race 1 using PacBio HiFi reads, Illumina short reads, and RNA-seq data, combined with stringent in silico filtering to remove host and microbial contaminants. The final assembly spans 151.7 Mbp across 67 scaffolds with 80.04% repeat content and 7,452 predicted genes. Functional annotation revealed effector-related features and gene categories consistent with biotrophic lifestyles. Comparative analyses with other P. xanthii genomes exposed variable contamination levels, underscoring the importance of strict data curation. Our work provides a reliable genomic reference for P. xanthii, enabling future research on pathogenicity, evolution, and host interactions in powdery mildew fungi and contributing to broader understanding of obligate biotrophic adaptation.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 6","pages":"868-875"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol of Bacterial Seedling Rot in Rice Plants Using Bacillus velezensis JBCS608 and Its Formulated Products. velezensis JBCS608及其制剂防治水稻细菌性秧苗腐病的研究

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-12-01 DOI: 10.5423/PPJ.OA.08.2025.0101

Jun An Kang, Yong Hoon Lee

{"title":"Biocontrol of Bacterial Seedling Rot in Rice Plants Using Bacillus velezensis JBCS608 and Its Formulated Products.","authors":"Jun An Kang, Yong Hoon Lee","doi":"10.5423/PPJ.OA.08.2025.0101","DOIUrl":"10.5423/PPJ.OA.08.2025.0101","url":null,"abstract":"Burkholderia glumae, the causal agent of bacterial panicle blight and seedling rot (BSR), is a growing threat to rice production, yet effective and sustainable control measures remain limited. In this study, we evaluated Bacillus velezensis JBCS608 (JBCS608) as a promising biocontrol agent for the management of BSR in rice plants. Treatment with JBCS608 at an optimal concentration of 1 × 10⁸ CFU/mL significantly reduced disease severity, achieving a control efficacy of up to 68.9%. The strain exhibited broad-spectrum antagonistic activity, inhibiting the growth of multiple seed-borne bacterial pathogens, including B. glumae, Burkholderia gladioli, and Burkholderia plantarii, as well as fungal pathogens such as Fusarium moniliforme. In addition to disease suppression, JBCS608 promoted rice seedling growth, likely through the production of indole-3-acetic acid and siderophores, and phosphate-solubilizing activity. For practical applications, we developed both talc-based wettable powder and molasses humic acid liquid formulations using JBCS608 endospores. Both formulations exhibited biocontrol efficacy comparable to that of fresh bacterial cells and maintained high cell viability for over 6 months under cold storage conditions. Collectively, our results indicate that JBCS608 is a potent and eco-friendly biocontrol and plant growth-promoting agent with strong potential for commercial use in the integrated management of BSR in rice.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 6","pages":"790-799"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic Sequencing of Maize Reveals Abundant Genomic RNA of a Comovirus, a Genus Previously Known to Infect Only Dicots. 玉米宏基因组测序揭示了一种科莫病毒的丰富基因组RNA，这种病毒以前只感染玉米。

IF 2.5 3区农林科学

Plant Pathology Journal Pub Date : 2025-10-01 DOI: 10.5423/PPJ.OA.06.2025.0077

Havva Ilbağı, Surapathrudu Kanakala, Rick Masonbrink, Zachary Lozier, W Allen Miller

{"title":"Metagenomic Sequencing of Maize Reveals Abundant Genomic RNA of a Comovirus, a Genus Previously Known to Infect Only Dicots.","authors":"Havva Ilbağı, Surapathrudu Kanakala, Rick Masonbrink, Zachary Lozier, W Allen Miller","doi":"10.5423/PPJ.OA.06.2025.0077","DOIUrl":"10.5423/PPJ.OA.06.2025.0077","url":null,"abstract":"To better understand the diversity of viral pathogens in Türkiye, a major exporter of cereals in Europe, we performed high-throughput sequencing of total RNA from maize plants collected in the Trakya region. Certain maize plants exhibiting mosaic and mottle symptoms, gathered from Tekirdağ province in Trakya, yielded large numbers of reads corresponding to the genome of a divergent strain of a comovirus, which corresponds to turnip ringspot virus (TuRSV), a recognized species of the genus Comovirus. This finding is unexpected because all known comoviruses infect only dicotyledonous species, and the known host range of TuRSV has been limited to plants in the Brassicaceae family. The nearly complete and partial nucleotide sequences of the bipartite genome of the maize isolate, as named TuRSVTR59, consist of 6,027 nt TuRSV-TR59 RNA1 and 3,920 nt TuRSV-TR59 RNA2, excluding poly (A) tails. RNA1 and RNA2 each encode a single ORF of 1,860 and 1,096 codons, respectively. Phylogenetic analysis demonstrated that TuRSV-TR59 from Türkiye clustered with other TuRSV isolates from diverse hosts and regions, showing highest identity to isolates from Germany, Czech Republic, and Croatia (80.56-77.77% and 92.09-90.50% nucleotide and amino acid sequence identities, respectively). The ability of TuRSV-TR59 isolate to infect maize was confirmed by reverse transcription polymerase chain reaction. Surveys in the Tekirdağ province of Türkiye, done in 2022-2025, revealed that 2 out of 145 maize samples (1.38%) and 8 out of 116 canola samples (6.89%) were found infected with TuRSV. This is the first report of a comovirus in maize from a monocotyledonous plant species.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 5","pages":"656-670"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12488379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0