Havva Ilbağı, Surapathrudu Kanakala, Rick Masonbrink, Zachary Lozier, W Allen Miller
{"title":"玉米宏基因组测序揭示了一种科莫病毒的丰富基因组RNA,这种病毒以前只感染玉米。","authors":"Havva Ilbağı, Surapathrudu Kanakala, Rick Masonbrink, Zachary Lozier, W Allen Miller","doi":"10.5423/PPJ.OA.06.2025.0077","DOIUrl":null,"url":null,"abstract":"<p><p>To better understand the diversity of viral pathogens in Türkiye, a major exporter of cereals in Europe, we performed high-throughput sequencing of total RNA from maize plants collected in the Trakya region. Certain maize plants exhibiting mosaic and mottle symptoms, gathered from Tekirdağ province in Trakya, yielded large numbers of reads corresponding to the genome of a divergent strain of a comovirus, which corresponds to turnip ringspot virus (TuRSV), a recognized species of the genus Comovirus. This finding is unexpected because all known comoviruses infect only dicotyledonous species, and the known host range of TuRSV has been limited to plants in the Brassicaceae family. The nearly complete and partial nucleotide sequences of the bipartite genome of the maize isolate, as named TuRSVTR59, consist of 6,027 nt TuRSV-TR59 RNA1 and 3,920 nt TuRSV-TR59 RNA2, excluding poly (A) tails. RNA1 and RNA2 each encode a single ORF of 1,860 and 1,096 codons, respectively. Phylogenetic analysis demonstrated that TuRSV-TR59 from Türkiye clustered with other TuRSV isolates from diverse hosts and regions, showing highest identity to isolates from Germany, Czech Republic, and Croatia (80.56-77.77% and 92.09-90.50% nucleotide and amino acid sequence identities, respectively). The ability of TuRSV-TR59 isolate to infect maize was confirmed by reverse transcription polymerase chain reaction. Surveys in the Tekirdağ province of Türkiye, done in 2022-2025, revealed that 2 out of 145 maize samples (1.38%) and 8 out of 116 canola samples (6.89%) were found infected with TuRSV. 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Certain maize plants exhibiting mosaic and mottle symptoms, gathered from Tekirdağ province in Trakya, yielded large numbers of reads corresponding to the genome of a divergent strain of a comovirus, which corresponds to turnip ringspot virus (TuRSV), a recognized species of the genus Comovirus. This finding is unexpected because all known comoviruses infect only dicotyledonous species, and the known host range of TuRSV has been limited to plants in the Brassicaceae family. The nearly complete and partial nucleotide sequences of the bipartite genome of the maize isolate, as named TuRSVTR59, consist of 6,027 nt TuRSV-TR59 RNA1 and 3,920 nt TuRSV-TR59 RNA2, excluding poly (A) tails. RNA1 and RNA2 each encode a single ORF of 1,860 and 1,096 codons, respectively. 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引用次数: 0
摘要
为了更好地了解欧洲主要谷物出口国t rkiye的病毒病原体多样性,我们对Trakya地区收集的玉米植株的总RNA进行了高通量测序。从特拉克亚省tekirdaak省采集的某些表现出花叶状和斑驳状症状的玉米植株产生了大量与一种科络病毒的不同毒株基因组相对应的reads,该毒株与科络病毒属的一种公认的芜菁环斑病毒(TuRSV)相对应。这一发现是出乎意料的,因为所有已知的共叶病毒只感染双子叶植物,而TuRSV的已知宿主范围仅限于芸苔科植物。该玉米分离物的近完整和部分核苷酸序列命名为TuRSVTR59,包括6,027 nt tursvv - tr59 RNA1和3,920 nt tursvv - tr59 RNA2,不包括poly (A)尾巴。RNA1和RNA2分别编码一个包含1860个和1096个密码子的ORF。系统发育分析表明,来自t rkiye的TuRSV- tr59与来自不同宿主和地区的其他TuRSV分离株聚集在一起,与来自德国、捷克共和国和克罗地亚的分离株具有最高的同源性(核苷酸和氨基酸序列分别为80.56-77.77%和92.09-90.50%)。通过逆转录聚合酶链反应证实了TuRSV-TR59分离株侵染玉米的能力。2022-2025年在基耶省特克尔达伊省进行的调查显示,145份玉米样本中有2份(1.38%)和116份油菜籽样本中有8份(6.89%)被发现感染了TuRSV。这是首次在玉米中发现来自单子叶植物的comovirus。
Metagenomic Sequencing of Maize Reveals Abundant Genomic RNA of a Comovirus, a Genus Previously Known to Infect Only Dicots.
To better understand the diversity of viral pathogens in Türkiye, a major exporter of cereals in Europe, we performed high-throughput sequencing of total RNA from maize plants collected in the Trakya region. Certain maize plants exhibiting mosaic and mottle symptoms, gathered from Tekirdağ province in Trakya, yielded large numbers of reads corresponding to the genome of a divergent strain of a comovirus, which corresponds to turnip ringspot virus (TuRSV), a recognized species of the genus Comovirus. This finding is unexpected because all known comoviruses infect only dicotyledonous species, and the known host range of TuRSV has been limited to plants in the Brassicaceae family. The nearly complete and partial nucleotide sequences of the bipartite genome of the maize isolate, as named TuRSVTR59, consist of 6,027 nt TuRSV-TR59 RNA1 and 3,920 nt TuRSV-TR59 RNA2, excluding poly (A) tails. RNA1 and RNA2 each encode a single ORF of 1,860 and 1,096 codons, respectively. Phylogenetic analysis demonstrated that TuRSV-TR59 from Türkiye clustered with other TuRSV isolates from diverse hosts and regions, showing highest identity to isolates from Germany, Czech Republic, and Croatia (80.56-77.77% and 92.09-90.50% nucleotide and amino acid sequence identities, respectively). The ability of TuRSV-TR59 isolate to infect maize was confirmed by reverse transcription polymerase chain reaction. Surveys in the Tekirdağ province of Türkiye, done in 2022-2025, revealed that 2 out of 145 maize samples (1.38%) and 8 out of 116 canola samples (6.89%) were found infected with TuRSV. This is the first report of a comovirus in maize from a monocotyledonous plant species.