Plant Pathology Journal最新文献_第7页

Transcriptome Comparison between Resistant and Susceptible Soybean Cultivars in Response to Inoculation of Phytophthora sojae. 抗感大豆品种对接种大豆疫霉的转录组比较

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.09.2024.0154

Sangrea Shim, In-Jeong Kang, Hee Jin You, Hangil Kim, Sungwoo Lee

{"title":"Transcriptome Comparison between Resistant and Susceptible Soybean Cultivars in Response to Inoculation of Phytophthora sojae.","authors":"Sangrea Shim, In-Jeong Kang, Hee Jin You, Hangil Kim, Sungwoo Lee","doi":"10.5423/PPJ.OA.09.2024.0154","DOIUrl":"10.5423/PPJ.OA.09.2024.0154","url":null,"abstract":"Phytophthora root and stem rot, caused by Phytophthora sojae, considerably reduces soybean yield worldwide. Our previous study identified two genomic regions on chromosome 18 (2.1-2.6 and 53.1-53.3 Mbp) that confer resistance to the P. sojae isolate 2457, through linkage analysis using progenies derived from the Daepung × Socheong2 population. These two regions contained 51 and 19 annotated genes, respectively. However, the specific gene responsible for resistance to P. sojae isolate 2457 has yet to be identified. In this study, we performed a comparative transcriptomic analysis of Socheong2 and Daepung, two Korean soybean varieties identified as resistant and susceptible to P. sojae isolate 2457, respectively. RNA sequencing was conducted on tissue samples collected at 0, 6, and 12 hours after inoculation (HAI), and significant differences in the expression of defense-related genes were observed across time points and between the two cultivars. Genes associated with the jasmonic acid, salicylic acid, ethylene, and systemic acquired resistance pathways were upregulated in both cultivars at 6 and 12 HAI compared to 0 HAI, with these biological processes were more strongly upregulated in Socheong2 compared to Daepung at 6 and 12 HAI. A comparison of differentially expressed genes (DEGs) and candidate genes within the previously identified QTL regions revealed an ortholog of the HS1 PRO-1 2 gene from Arabidopsis thaliana among the upregulated DEGs in Socheong2, particularly at 12 HAI compared to 0 HAI. This study will aid in targeted breeding efforts to develop soybean varieties with improved resistance to P. sojae.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"641-655"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Biocontrol Efficacy of Bacillus velezensis GYUN-1190 against Apple Bitter Rot. 香蕉芽孢杆菌gun -1190对苹果苦腐病的防治效果研究

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.05.2024.0076

Hyeonjin Park, Younmi Lee, Kotnala Balaraju, Jungyeon Kim, Yongho Jeon

{"title":"Characterization and Biocontrol Efficacy of Bacillus velezensis GYUN-1190 against Apple Bitter Rot.","authors":"Hyeonjin Park, Younmi Lee, Kotnala Balaraju, Jungyeon Kim, Yongho Jeon","doi":"10.5423/PPJ.OA.05.2024.0076","DOIUrl":"10.5423/PPJ.OA.05.2024.0076","url":null,"abstract":"The application of synthetic fungicides has resulted in environmental pollution and adverse effects on non-target species. To reduce the use of agrochemicals, crop disease management requires microbial biological control agents. Bacillus-related genera produce secondary metabolites to control fungal pathogens. Bacillus velezensis GYUN-1190, isolated from soil, showed antagonistic activity against Colletotrichum fructicola, the apple anthracnose pathogen. Volatile organic compounds and culture filtrate (CF) from GYUN-1190 inhibited C. fructicola growth in vitro, by 80.9% and 30.25%, respectively. The CF of GYUN-1190 inhibited pathogen spore germination more than cell suspensions at 10 8 cfu/ml. Furthermore, GYUN-1190 CF is effective in inhibiting C. fructicola mycelial growth in vitro, and it suppresses apple fruit bitter rot more effectively than GYUN-1190 cell suspensions and pyraclostrobin in planta. The mycelial growth of C. fructicola was completely inhibited 48 h after immersion into the CF, in compared with positive controls and GYUN-1190 cell suspensions. The genetic mechanism underlying the biocontrol features of GYUN-1190 was defined using its whole-genome sequence, which was closely compared to similar strains. It consisted of 4,240,653 bp with 45.9% GC content, with 4,142 coding sequences, 87 tRNA, and 28 rRNA genes. The genomic investigation found 14 putative secondary metabolite biosynthetic gene clusters. The investigation suggests that B. velezensis GYUN-1190 might be more effective than chemical fungicides and could address its potential as a biological control agent.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"681-695"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening Method to Identify Watermelon Cultivars Resistant to Acidovorax citrulli, the Cause of Bacterial Fruit Blotch. 西瓜抗细菌性果实斑病的筛选方法研究。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.08.2024.0122

Soo Min Lee, Gahyeon Kim, Hun Kim, Gyung Ja Choi

{"title":"Screening Method to Identify Watermelon Cultivars Resistant to Acidovorax citrulli, the Cause of Bacterial Fruit Blotch.","authors":"Soo Min Lee, Gahyeon Kim, Hun Kim, Gyung Ja Choi","doi":"10.5423/PPJ.OA.08.2024.0122","DOIUrl":"10.5423/PPJ.OA.08.2024.0122","url":null,"abstract":"Acidovorax citrulli is a causative pathogen for bacterial fruit blotch (BFB) in Cucurbitaceae, including watermelon. The most effective method to control this plant disease is to cultivate resistant cultivars. Herein, this study aimed to establish an efficient screening method to determine the resistance of watermelon cultivars against A. citrulli. To this end, we explored the virulence of seven A. citrulli isolates belonging to clonal complex group I or II based on gltA gene analysis. Furthermore, we evaluated the BFB occurrence in the seedlings of two arbitrarily selected watermelon cultivars according to the growth stage of the watermelon seedlings, inoculum concentration, and incubation temperature after inoculation in a humidity chamber. Taken together, we established the following method to determine the resistance of watermelon cultivars against A. citrulli: watermelon seedlings at the fully expanded two-leaf stage can be spray-inoculated with an A. citrulli bacterial suspension at a concentration of 1.0 × 106 cfu/ml; after incubation for 48 h at 28℃ in a humidity chamber, the plants were cultivated in a growth chamber at 25℃ with 80% relative humidity under a 12-h light/dark cycle; and BFB occurrence on the plants can be investigated at 7 dpi by visual estimation of the diseased leaf area (%). Based on these experimental methods, we investigated the resistance degree of 43 commercial watermelon cultivars against A. citrulli KACC 17005. Therefore, our results can provide important information for the development of resistant cultivars against A. citrulli.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"593-602"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel and Advanced Diagnostic Approach toward Paracidovorax citrulli Causing Bacterial Fruit Blotch in Watermelon by Direct SYBR Green Real-Time PCR Assay. 直接SYBR绿色实时荧光定量PCR法诊断西瓜细菌性果斑病的新方法

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.08.2024.0125

Hyeonheui Ham, Bang Wool Lee, Kyongnim Kim, Woohyung Lee, Yong Hwan Lee, Dong Suk Park

{"title":"A Novel and Advanced Diagnostic Approach toward Paracidovorax citrulli Causing Bacterial Fruit Blotch in Watermelon by Direct SYBR Green Real-Time PCR Assay.","authors":"Hyeonheui Ham, Bang Wool Lee, Kyongnim Kim, Woohyung Lee, Yong Hwan Lee, Dong Suk Park","doi":"10.5423/PPJ.OA.08.2024.0125","DOIUrl":"10.5423/PPJ.OA.08.2024.0125","url":null,"abstract":"Bacterial fruit blotch (BFB) caused by Paracidovorax citrulli is a devastating disease in cucurbit hosts such as watermelon. P. citrulli is a seed-borne pathogen, and contaminated seeds are the primary inoculum. Because it is difficult to control BFB after the emergence of the disease, it is essential to detect the pathogen and remove infected plant materials, including seeds, in the early stages. In this study, we developed a direct SYBR Green real-time polymerase chain reaction (PCR) method using a new species-specific marker for detecting P. citrulli with high specificity and sensitivity. The genome information of P. citrulli and related species was collected and compared to retrieve the P. citrulli-specific gene. The primer set RS01560-164 was designed based on the selected gene and tested for specificity and sensitivity using cloned DNA, genomic DNA, cell suspension, and suspensions obtained from infected watermelon cotyledons. Our primer set detected only P. citrulli from the closely related species with a detection limit of cloned DNA at 1.46 × 103 copies/µl, gDNA at 500 fg/µl, and cell suspension at 1.4 × 104 cfu/ml by real-time PCR. Our method also detected P. citrulli from diseased plants without the need for a DNA extraction phase. Therefore, the primer set and real-time PCR methods newly developed in this study could be applied for the specific detection of P. citrulli in plants or seeds with high sensitivity and robustness, providing the potential to manage BFB in cucurbits.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"671-680"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Antifungal Activity-Guided Analysis of Actinostemma lobatum Extracts through Serial Sub-fractions. 勘误：通过系列亚组分对放线素草提取物的抗真菌活性进行指导分析。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.ER.11.2023.0152

Seonwoo Choi, Song Hee Lee, Buyng Su Hwang, Young Taek Oh, Junhyun Jeon

引用次数: 0

Mycological and Genomic Characterization of Fusarium vorosii, a Potentially Pathogenic Fungus, Isolated from Field Crops and Weeds in Korea. 从韩国大田作物和杂草中分离的一种潜在致病性真菌镰刀菌的真菌学和基因组学特征。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.08.2024.0121

Eunji Jeong, Jae Yun Lim, Jeong-Ah Seo

{"title":"Mycological and Genomic Characterization of Fusarium vorosii, a Potentially Pathogenic Fungus, Isolated from Field Crops and Weeds in Korea.","authors":"Eunji Jeong, Jae Yun Lim, Jeong-Ah Seo","doi":"10.5423/PPJ.OA.08.2024.0121","DOIUrl":"10.5423/PPJ.OA.08.2024.0121","url":null,"abstract":"Fusarium vorosii (Fv) is one of the least studied species of the Fusarium graminearum species complex, a major plant pathogen causing Fusarium head blight (FHB) in a variety of crops. In this study, we isolated 12 strains of Fv from cereal samples with FHB symptoms and gramineous weeds. Trichothecene genotyping of Fv strains showed that 10 strains were nivalenol (NIV) type and 2 strains were 15-acetyldeoxynivalenol (15ADON) type. Fv strains have similar mycological characteristics to Fusarium asiaticum, a major FHB pathogen of rice in Asia, however, asexual sporulation was at least 100 to 1,000 times higher in Fv. In comparison of pathogenicity, the Fv-15ADON type was more pathogenic than the NIV type in both rice and wheat, and had a similar level of pathogenicity as the F. asiaticum-NIV type. Among the 12 Fv strains, two representative ones, Fv-NIV type RN1 and Fv-15ADON type W15A1, were selected and their whole genomes were sequenced and analyzed. Complete genome sequences of two Fv strains, RN1 and W15A1, were assembled at the chromosome level with high quality compared to known Fv genomes. The genome data of the two Fv strains were compared with the reference strains already known. As a result of comparative genome analysis, it was found that they are phylogenetically related according to the trichothecene biosynthetic gene cluster, that is, toxin chemotype. Through this study, we provided important information about Fv species that can be potential pathogens in domestic crops about biological and genomic characteristics.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"656-670"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different Infection Structures and Point Mutation of Diaporthe citri Showing Resistant against Systemic Fungicides. 柑桔不同侵染结构及点突变对系统杀菌剂的抗性。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.07.2024.0112

Zar Zar Soe, Yong Ho Shin, Yong Chull Jeun

{"title":"Different Infection Structures and Point Mutation of Diaporthe citri Showing Resistant against Systemic Fungicides.","authors":"Zar Zar Soe, Yong Ho Shin, Yong Chull Jeun","doi":"10.5423/PPJ.OA.07.2024.0112","DOIUrl":"10.5423/PPJ.OA.07.2024.0112","url":null,"abstract":"Infection behaviors of some susceptible and resistant Diaporthe citri isolates against two fungicides such as benomyl and kresoxim-methyl were observed on citrus leaves. On the chemically unsprayed leaves, there were no differences in not only the conidial germination rate but also hyphal growth between the resistant and susceptible isolates. However, on the benomyl or kresoximmethyl-pretreated leaves, both conidial germination rate and hyphal growth of the resistant isolates were not decreased, which was different with those of the susceptible isolates. These unchanging germination rate and hyphal growth of the resistant isolates, resulting in the lack of chemical efficacy, coincided with the previous studies in which strong infection was observed by the resistant isolates after pre-treatment with each chemical. On the other hand, a point mutation occurred at 198th amino acid of β-tubulin gene in the resistant isolate NEL21-2 which may cause the resistant against benomyl. However, no mutation was found in cytochrome b in the resistant isolates indicating other genomic changes may be responsible for the resistant against kresoxim-methyl.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"603-614"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Multiplex PCR to Simultaneously Detect Four Problematic Pathogens in Cymbidium kanran. 同时检测坎然蕙兰四种问题病原菌的多重PCR方法的建立。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.07.2024.0101

Jiwon Kim, Sunyung Yoon, Seungtae Kang, Ho Bang Kim, Kyung-Hwan Boo

{"title":"Development of a Multiplex PCR to Simultaneously Detect Four Problematic Pathogens in Cymbidium kanran.","authors":"Jiwon Kim, Sunyung Yoon, Seungtae Kang, Ho Bang Kim, Kyung-Hwan Boo","doi":"10.5423/PPJ.OA.07.2024.0101","DOIUrl":"10.5423/PPJ.OA.07.2024.0101","url":null,"abstract":"Colletotrichum gloeosporioides (Cg), Pestalotiopsis sp. (Ps), Fusarium oxysporum (Fo), and Fusarium proliferatum (Fp) are pathogens that cause various diseases of Cymbidium kanran. Species identification based on the morphological characteristics of pathogen-infected orchids is very complex and difficult; therefore, specific and reliable diagnostic methods are needed. Here, we developed a multiplex polymerase chain reaction (PCR) assay for simultaneous detection of Cg, Ps, Fo, and Fp. Four pairs of pathogen-specific primers were designed based on the internal transcribed spacer sequences of each pathogen, generating fragments of 480, 407, 321, and 279 bp, respectively. The multiplex PCR assay using these four pairs of mixed primers effectively detected the four pathogens simultaneously, with a sensitivity of 0.1 ng genomic DNA/pathogen. Application of the optimized multiplex PCR assay to symptomatic C. kanran leaf samples effectively detected single or multiple pathogens. These results indicate that the multiplex PCR developed in this study is not only a rapid and reliable method for detecting four problematic pathogens in C. kanran, but also serves as a very useful tool for early diagnosis.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"569-577"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of TaqMan-Based Real-Time qPCR Method for Accurate Detection and Quantification of Citrus Psorosis Virus and Cytoplasmic-Type Citrus Leprosis Virus in Saplings. 基于taqman的柑橘病病毒和细胞质型柑橘麻风病毒实时荧光定量检测方法的建立

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.09.2024.0134

Minhue Jung, Na Hee Kim, Seung Hyeon Oh, Kook-Hyung Kim

{"title":"Development of TaqMan-Based Real-Time qPCR Method for Accurate Detection and Quantification of Citrus Psorosis Virus and Cytoplasmic-Type Citrus Leprosis Virus in Saplings.","authors":"Minhue Jung, Na Hee Kim, Seung Hyeon Oh, Kook-Hyung Kim","doi":"10.5423/PPJ.OA.09.2024.0134","DOIUrl":"10.5423/PPJ.OA.09.2024.0134","url":null,"abstract":"In 2022, citrus fruits were the second most widely produced fruit globally, highlighting their significant role in the fruit industry. However, due to their clonal propagation, these fruits are highly susceptible to viral infections, posing challenges for growers. In response to the booming nursery market, the Korean plant quarantine station reported over 80 million sapling stocks, with 15% being discarded after rigorous inspection due to contamination or disease. As the global nursery trade continues to expand, there is an urgent need for a fast and accurate diagnostic tool to ensure the health of plant stocks. In this study, we developed a TaqMan-based real-time reverse transcription-quantitative PCR assay specifically designed to detect two critical citrus viruses: citrus psorosis virus and citrus leprosis virus C. Our assay demonstrated the capability to detect virus sequences with as few as 30 copies, maintaining high PCR efficiency with RNA extracted from both twig and leaf tissues. Additionally, we incorporated an artificial sequence into the positive controls, which effectively served as a marker for detecting potential sample contamination. This comprehensive diagnostic system promises to enhance plant quarantine measures and phytosanitation practices, providing a reliable and efficient solution to safeguard citrus crops from viral threats.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"625-632"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Tomato Seed Endophytic Bacteria as Growth Promoters and Potential Biocontrol Agents. 番茄种子内生细菌作为生长促进剂和潜在生物防治剂的特性研究。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.09.2024.0142

Mehwish Roy, Byeonghyeok Kang, Seongeun Yang, Heeyun Choi, Kihyuck Choi

{"title":"Characterization of Tomato Seed Endophytic Bacteria as Growth Promoters and Potential Biocontrol Agents.","authors":"Mehwish Roy, Byeonghyeok Kang, Seongeun Yang, Heeyun Choi, Kihyuck Choi","doi":"10.5423/PPJ.OA.09.2024.0142","DOIUrl":"10.5423/PPJ.OA.09.2024.0142","url":null,"abstract":"Endophytic bacteria residing within plant seeds are increasingly recognized for their potential to enhance plant growth and provide biocontrol against pathogens. Despite this, seed-borne endophytes remain underexplored in many crops, including tomato. In this study, we isolated and characterized bacterial endophytes from tomato seeds and evaluated their plant growth-promoting traits and antifungal activities. The taxonomic analysis of the Hawaii 7996 tomato seed endophyte collection revealed a diverse community, predominantly from the phylum Bacillota, with Paenibacillaceae and Bacillaceae as the most abundant families. Among the 35 unique strains identified, 19 produced indole-3-acetic acid, four exhibited siderophore production, and 12 could solubilize phosphate. These traits contribute to growth promotion and disease suppression in plants. In the plant growth promotion assay, several bacterial strains, notably Streptomyces olivaceus (BHM1), Streptomyces variegatus (BHM3), Bacillus stercoris (BHR2), and Moraxella osloensis (YHT4-1), demonstrated significant potential for tomato cultivation by positively affecting fresh weight, stem length, and root length. These strains consistently promoted growth across all three parameters evaluated in this study. Furthermore, several strains exhibited strong antifungal activity against major tomato pathogens, including Fusarium oxysporum race 1 and 2, and Botrytis cinerea. Notably, Bacillus subtilis (BHN1), Bacillus stercoris (BHR2), and Paenibacillus peoriae (YHR2-1) showed broad-spectrum antifungal efficacy. Our findings highlight the potential of seed-associated endophytic bacteria as growth promoters and biological control agents, offering promising avenues for sustainable agricultural practices.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"578-592"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0