Plant Pathology Journal最新文献_第7页

Proteomic and Phenotypic Analyses of a Putative YggS Family Pyridoxal Phosphate-Dependent Enzyme in Acidovorax citrulli. 瓜梨YggS家族吡哆醛磷酸依赖酶的蛋白质组学和表型分析。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-06-01 DOI: 10.5423/PPJ.OA.03.2023.0050

Lynn Heo, Yongmin Cho, Junhyeok Choi, Jeongwook Lee, Yoobin Han, Sang-Wook Han

{"title":"Proteomic and Phenotypic Analyses of a Putative YggS Family Pyridoxal Phosphate-Dependent Enzyme in Acidovorax citrulli.","authors":"Lynn Heo, Yongmin Cho, Junhyeok Choi, Jeongwook Lee, Yoobin Han, Sang-Wook Han","doi":"10.5423/PPJ.OA.03.2023.0050","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2023.0050","url":null,"abstract":"Acidovorax citrulli (Ac) is a phytopathogenic bacterium that causes bacterial fruit blotch (BFB) in cucurbit crops, including watermelon. However, there are no effective methods to control this disease. YggS family pyridoxal phosphate-dependent enzyme acts as a coenzyme in all transamination reactions, but its function in Ac is poorly understood. Therefore, this study uses proteomic and phenotypic analyses to characterize the functions. The Ac strain lacking the YggS family pyridoxal phosphate-dependent enzyme, AcΔyppAc(EV), virulence was wholly eradicated in geminated seed inoculation and leaf infiltration. AcΔyppAc(EV) propagation was inhibited when exposed to L-homoserine but not pyridoxine. Wild-type and mutant growth were comparable in the liquid media but not in the solid media in the minimal condition. The comparative proteomic analysis revealed that YppAc is primarily involved in cell motility and wall/membrane/envelop biogenesis. In addition, AcΔyppAc(EV) reduced biofilm formation and twitching halo production, indicating that YppAc is involved in various cellular mechanisms and possesses pleiotropic effects. Therefore, this identified protein is a potential target for developing an efficient anti-virulence reagent to control BFB.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 3","pages":"235-244"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/d0/ppj-oa-03-2023-0050.PMC10265119.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9994500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antifungal Activity of Green and Chemically Synthesized ZnO Nanoparticles against Alternaria citri, the Causal Agent Citrus Black Rot. 绿色和化学合成氧化锌纳米颗粒对柑桔黑腐病病原菌柑橘交替病菌的抑菌活性研究。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-06-01 DOI: 10.5423/PPJ.OA.02.2023.0035

Hazem S Elshafie, Ali Osman, Mahmoud M El-Saber, Ippolito Camele, Entsar Abbas

{"title":"Antifungal Activity of Green and Chemically Synthesized ZnO Nanoparticles against Alternaria citri, the Causal Agent Citrus Black Rot.","authors":"Hazem S Elshafie, Ali Osman, Mahmoud M El-Saber, Ippolito Camele, Entsar Abbas","doi":"10.5423/PPJ.OA.02.2023.0035","DOIUrl":"https://doi.org/10.5423/PPJ.OA.02.2023.0035","url":null,"abstract":"Citrus black rot is a serious disease of citrus plants caused by Alternaria citri. The current study aimed to synthesize zinc oxide nanoparticles (ZnO-NPs) by chemically or green method and investigate their antifungal activity against A. citri. The sizes of synthesized as measured by transmission electron microscope of ZnO-NPs were 88 and 65 nm for chemical and green methods, respectively. The studied prepared ZnO-NPs were applied, in vitro and in situ, at different concentrations (500, 1,000, and 2,000 µg/ml) in post-harvest treatment on navel orange fruits to verify the possible control effect against A. citri. Results of in vitro assay demonstrated that, at concentration 2,000 µg/ml, the green ZnO-NPs was able to inhibit about 61% of the fungal growth followed by 52% of chemical ZnO-NPs. In addition, scanning electron microscopy of A. citri treated in vitro with green ZnO-NPs showed swelling and deformation of conidia. Results showed also that, using a chemically and green ZnO-NPs at 2,000 µg/ml in situ in post-harvest treatment of orange, artificially-infected with A. citri, has reduced the disease severity to 6.92% and 9.23%, respectively, compared to 23.84% of positive control (non-treated fruits) after 20 days of storage. The out findings of this study may contribute to the development of a natural, effective, and eco-friendly strategy for eradicating harmful phytopathogenic fungi.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 3","pages":"265-274"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/16/cb/ppj-oa-02-2023-0035.PMC10265117.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9994499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Characterization of Diplodia parva and Diplodia crataegicola Causing Black Rot of Chinese Quince. 引起太子黑腐病的小双plodia和大黄双plodia的鉴定与鉴定。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-06-01 DOI: 10.5423/PPJ.OA.03.2023.0039

Sungmun Kwon, Jungyeon Kim, Younmi Lee, Kotnala Balaraju, Yongho Jeon

{"title":"Identification and Characterization of Diplodia parva and Diplodia crataegicola Causing Black Rot of Chinese Quince.","authors":"Sungmun Kwon, Jungyeon Kim, Younmi Lee, Kotnala Balaraju, Yongho Jeon","doi":"10.5423/PPJ.OA.03.2023.0039","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2023.0039","url":null,"abstract":"Fungal isolates from infected Chinese quince trees were found to cause black rot in Yeongcheon, Gyeongsangbuk Province, Korea. The quince leaves withered and turned reddish-brown and fruits underwent black mummification. To elucidate the cause of these symptoms, the pathogen was isolated from infected leaf and fruit tissues on potato dextrose agar and Levan media. Several fungal colonies forming a fluffy white or dark gray mycelium and two types of fungi forming an aerial white mycelium, growing widely at the edges, were isolated. Microscopic observations, investigation of fungal growth characteristics on various media, and molecular identification using an internal transcribed spacer, β-tubulin, and translation elongation factor 1-α genes were performed. The fungal pathogens were identified as Diplodia parva and Diplodia crataegicola. Pathogenicity tests revealed that the pathogen-inoculated fruits exhibited a layered pattern, turning brown rotting; leaves showed circular brown necrotic lesions. The developed symptoms were similar to those observed in the field. Fungal pathogens were reisolated to fulfill Koch's postulates. Apples were inoculated with fungal pathogens to investigate the host range. Strong pathogenicity was evident in the fruits, with browning and rotting symptoms 3 days after inoculation. To determine pathogen control, a fungicidal sensitivity test was conducted using four registered fungicides. Thiophanate-methyl, propineb, and tebuconazole inhibited the mycelial growth of pathogens. To the best of our knowledge, this is the first report on the isolation and identification of the fungal pathogens D. parva and D. crataegicola from infected fruits and leaves of Chinese quince, causing black rot disease in Korea.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 3","pages":"275-289"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/62/e7/ppj-oa-03-2023-0039.PMC10265116.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9691953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potency of Abamectin Formulations against the Pine Wood Nematode, Bursaphelenchus xylophilus. 阿维菌素制剂对松材线虫的防效研究。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-06-01 DOI: 10.5423/PPJ.OA.02.2023.0023

Jong-Won Lee, Abraham Okki Mwamula, Jae-Hyuk Choi, Ho-Wook Lee, Yi Seul Lee, Jin-Hyo Kim, Dong Woon Lee

{"title":"The Potency of Abamectin Formulations against the Pine Wood Nematode, Bursaphelenchus xylophilus.","authors":"Jong-Won Lee, Abraham Okki Mwamula, Jae-Hyuk Choi, Ho-Wook Lee, Yi Seul Lee, Jin-Hyo Kim, Dong Woon Lee","doi":"10.5423/PPJ.OA.02.2023.0023","DOIUrl":"https://doi.org/10.5423/PPJ.OA.02.2023.0023","url":null,"abstract":"Abamectin offers great protection against Bursaphelenchus xylophilus, a well-known devastating pathogen of pine tree stands. Trunk injection of nematicides is currently the most preferred method of control. This study aimed to evaluate the potency of the commonly used formulations of abamectin against B. xylophilus. Twenty-one formulations of abamectin were evaluated by comparing their sublethal toxicities and reproduction inhibition potentials against B. xylophilus. Nematodes were treated with diluted formulation concentrations in multi-well culture plates. And, populations pre-exposed to pre-determined concentrations of the formulations were inoculated onto Botrytis cinerea culture, and in pine twig cuttings. Potency was contrastingly different among formulations, with LC95 of 0.00285 and 0.39462 mg/ml for the most, and the least potent formulation, respectively. Paralysis generally occurred at an application dose of 0.06 μg/ml or higher, and formulations with high sublethal toxicities caused significant paralysis levels at the tested doses, albeit the variations. Nematode reproduction was evident at lower doses of 0.00053-0.0006 μg/ml both on Botrytis cinerea and pine twigs, with significant variations among formulations. Thus, the study highlighted the inconsistencies in the potency of similar product formulations with the same active ingredient concentration against the target organism, and the need to analyze the potential antagonistic effects of the additives used in formulations.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 3","pages":"290-302"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f0/95/ppj-oa-02-2023-0023.PMC10265120.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9691950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of an Agroinfectious Clone of a Korean Isolate of Sweet Potato Symptomless Virus 1 and Comparison of Its Infectivity According to Agrobacterium tumefaciens Strains in Nicotiana benthamiana. 甘薯无症状病毒1号韩国分离株农染性克隆的构建及其与本菌烟中农染性的比较

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-06-01 DOI: 10.5423/PPJ.OA.12.2022.0168

Phuong T Ho, Hee-Seong Byun, Thuy T B Vo, Aamir Lal, Sukchan Lee, Eui-Joon Kil

{"title":"Construction of an Agroinfectious Clone of a Korean Isolate of Sweet Potato Symptomless Virus 1 and Comparison of Its Infectivity According to Agrobacterium tumefaciens Strains in Nicotiana benthamiana.","authors":"Phuong T Ho, Hee-Seong Byun, Thuy T B Vo, Aamir Lal, Sukchan Lee, Eui-Joon Kil","doi":"10.5423/PPJ.OA.12.2022.0168","DOIUrl":"https://doi.org/10.5423/PPJ.OA.12.2022.0168","url":null,"abstract":"Sweet potato symptomless virus 1 (SPSMV-1) is a single-stranded circular DNA virus, belonging to the genus Mastrevirus (family Geminiviridae) that was first identified on sweet potato plants in South Korea in 2012. Although SPSMV-1 does not induce distinct symptoms in sweet potato plants, its co-infection with different sweet potato viruses is highly prevalent, and thus threatens sweet potato production in South Korea. In this study, the complete genome sequence of a Korean isolate of SPSMV-1 was obtained by Sanger sequencing of polymerase chain reaction (PCR) amplicons from sweet potato plants collected in the field (Suwon). An infectious clone of SPSMV-1 (1.1-mer) was constructed, cloned into the plant expression vector pCAMBIA1303, and agro-inoculated into Nicotiana benthamiana using three Agrobacterium tumefaciens strains (GV3101, LBA4404, and EHA105). Although no visual differences were observed between the mock and infected groups, SPSMV-1 accumulation was detected in the roots, stems, and newly produced leaves through PCR. The A. tumefaciens strain LBA4404 was the most effective at transferring the SPSMV-1 genome to N. benthamiana. We confirmed the viral replication in N. benthamiana samples through strand-specific amplification using virion-sense- and complementary-sense-specific primer sets.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 3","pages":"255-264"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/72/93/ppj-oa-12-2022-0168.PMC10265118.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9691951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Identification of Jasmine Virus H Infecting Ixora coccinea by Nanopore Metatranscriptomics. 利用纳米孔超转录组学快速鉴定感染瓢虫的茉莉病毒H。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-06-01 DOI: 10.5423/PPJ.NT.03.2023.0037

Sung-Woong Kim, Hyo-Jeong Lee, Sena Choi, In-Sook Cho, Rae-Dong Jeong

{"title":"Rapid Identification of Jasmine Virus H Infecting Ixora coccinea by Nanopore Metatranscriptomics.","authors":"Sung-Woong Kim, Hyo-Jeong Lee, Sena Choi, In-Sook Cho, Rae-Dong Jeong","doi":"10.5423/PPJ.NT.03.2023.0037","DOIUrl":"https://doi.org/10.5423/PPJ.NT.03.2023.0037","url":null,"abstract":"The global climate change and international trade have facilitated the movement of plants across borders, increasing the risk of introducing novel plant viruses in new territories. Ixora coccinea exhibited virus-like foliar symptoms, including mosaic and mild mottle. An Oxford Nanopore Technologies-based compact and portable MinION platform was used to identify the causal viral pathogen. The complete genome sequence of jasmine virus H (JaVH; 3867 nt, JaVH-CNU) was determined and found to share 88.4-90.3% nucleotide identity with that of Jasminum sambac JaVH isolate in China. Phylogenetic analysis based on the complete amino acid sequences of RNA-dependent RNA polymerase and coat protein revealed that JaVH-CNU was grouped separately with other JaVH isolates. This is the first report of a natural JaVH infection of >i<I. coccinea. The application of rapid nanopore sequencing for plant virus identification was demonstrated and is expected to provide accurate and rapid diagnosis for virus surveillance.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 3","pages":"303-308"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/23/72/ppj-nt-03-2023-0037.PMC10265114.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9994498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivar Resistance of Korean Breeding Cut-Rose against Crown Gall by Agrobacterium tumefaciens Evaluated by an In Vitro Inoculation. 用离体接种法评价韩国品种切花月季对农杆菌冠瘿病的抗性。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-04-01 DOI: 10.5423/PPJ.OA.07.2022.0095

Serah Lim, Se Chul Chun, Jin-Won Kim

{"title":"Cultivar Resistance of Korean Breeding Cut-Rose against Crown Gall by Agrobacterium tumefaciens Evaluated by an In Vitro Inoculation.","authors":"Serah Lim, Se Chul Chun, Jin-Won Kim","doi":"10.5423/PPJ.OA.07.2022.0095","DOIUrl":"https://doi.org/10.5423/PPJ.OA.07.2022.0095","url":null,"abstract":"Rose crown gall caused by Agrobacterium tumefaciens is a major disease that damages the production of cut-roses in Korea. The effective prevention methods for this disease include the use of resistant varieties. This study was conducted to evaluate the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease with nodal explants in vitro. Among 180 A. tumefaciens strains, pathogenic strain RC12 was selected as an inoculant strain. The strain RC12 was identified based on characteristics of some selective media, pathogenicity test, and polymerase chain reaction analysis. Forty rose cultivars formed tumors on explants inoculated with A. tumefaciens RC12. However, 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to A. tumefaciens RC12 without forming any tumors. Six cultivars with tumor formation rates of over 30% formed initial tumors within 23 days after inoculation. Six cultivars with low tumor formation rates of around 5% formed initial tumors after 28 days of inoculation. It was found that gall formation rate was highly correlated with the initial gall formation period. Thus, the relationship between the period of gall formation and the rate of gall formation could be useful for assessing resistance to crown gall disease. In vitro inoculation methods could be used to evaluate resistance of cut-rose cultivars to crown gall diseases.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 2","pages":"220-227"},"PeriodicalIF":2.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ec/03/ppj-oa-07-2022-0095.PMC10102570.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Alternative Method to Evaluate Resistance to Pear Scab (Venturia nashicola). 梨痂病(Venturia nashicola)抗性评价方法研究。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-04-01 DOI: 10.5423/PPJ.NT.01.2023.0006

Kyungho Won, Eu Ddeum Choi, Keumsun Kim, Hae Won Jung, Il Sheob Shin, Seongsig Hong, Cécile Segonzac, Young Jin Kim

引用次数: 0

Defense Inducer Compounds Up-regulated the Peroxidase, Polyphenol Oxidase, and Total Phenol Activities against Spot Blotch Disease of Wheat. 防御诱导剂化合物可提高小麦过氧化物酶、多酚氧化酶和总酚的活性，防治小麦斑点病。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2023-04-01 DOI: 10.5423/PPJ.OA.06.2022.0080

Puja Kumari, Chandrashekhar Azad, Ravi Ranjan Kumar, Jyoti Kumari, Kumar Aditya, Amarendra Kumar

{"title":"Defense Inducer Compounds Up-regulated the Peroxidase, Polyphenol Oxidase, and Total Phenol Activities against Spot Blotch Disease of Wheat.","authors":"Puja Kumari, Chandrashekhar Azad, Ravi Ranjan Kumar, Jyoti Kumari, Kumar Aditya, Amarendra Kumar","doi":"10.5423/PPJ.OA.06.2022.0080","DOIUrl":"10.5423/PPJ.OA.06.2022.0080","url":null,"abstract":"Spot blotch disease of wheat caused by Bipolaris sorokiniana (Sacc.) Shoem is considered as an economically important disease which affects all the growing stages of wheat crop. Therefore, it is important to search some effective management strategies against the spot blotch pathogen. Some synthetic elicitor compounds (salicylic acid, isonicotinic acid, and chitosan) and nano-particles (silver and aluminum) were tested against the pathogen to observe the change in biochemical activity and defense action of wheat plant against spot blotch disease. All the tested elicitor compounds and nano-particles showed a significant increase in activity of peroxidase, polyphenol oxidase (PPO), and total phenol over control. The highest increase in activity of peroxidase was recorded at 72 h from chitosan at 2 mM and 96 h from silver nano-particle at 100 ppm. Maximum PPO and total phenol activity were recorded from chitosan at 2 mM and silver nano-particle at 100 ppm as compared to pathogen-treated and healthy control. The lowest percent disease index, lowest no. of spots/leaf, and no. of infected leaves/plant were found in silver nano-particle at 100 ppm and chitosan at 2 mM, respectively. The use of defense inducer compounds results in significantly up-regulated enzymatic activity and reduced spot blotch disease. Therefore, chitosan and silver nano-particle could be used as alternative methods for the management of spot blotch disease.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 2","pages":"159-170"},"PeriodicalIF":1.8,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4f/22/ppj-oa-06-2022-0080.PMC10102565.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Genomic Analysis and Rapid Molecular Detection of Xanthomonas euvesicatoria Using Unique ATP-Dependent DNA Helicase recQ, hrpB1, and hrpB2 Genes Isolated from Physalis pubescens in China. 利用短毛Physalis pubescens中独特的atp依赖DNA解旋酶recQ、hrpB1和hrpB2基因的比较基因组分析和快速分子检测研究

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-04-01 DOI: 10.5423/PPJ.OA.08.2022.0119

Faisal Siddique, Yang Mingxiu, Xu Xiaofeng, Ni Zhe, Haseeb Younis, Peng Lili, Zhang Junhua

{"title":"Comparative Genomic Analysis and Rapid Molecular Detection of Xanthomonas euvesicatoria Using Unique ATP-Dependent DNA Helicase recQ, hrpB1, and hrpB2 Genes Isolated from Physalis pubescens in China.","authors":"Faisal Siddique, Yang Mingxiu, Xu Xiaofeng, Ni Zhe, Haseeb Younis, Peng Lili, Zhang Junhua","doi":"10.5423/PPJ.OA.08.2022.0119","DOIUrl":"https://doi.org/10.5423/PPJ.OA.08.2022.0119","url":null,"abstract":"Ground cherry (Physalis pubescens) is the most prominent species in the Solanaceae family due to its nutritional content, and prospective health advantages. It is grown all over the world, but notably in northern China. In 2019 firstly bacterial leaf spot (BLS) disease was identified on P. pubescens in China that caused by both BLS pathogens Xanthomonas euvesicatoria pv. euvesicatoria resulted in substantial monetary losses. Here, we compared whole genome sequences of X. euvesicatoria to other Xanthomonas species that caused BLS diseases for high similarities and dissimilarities in genomic sequences through average nucleotide identity (ANI) and BLAST comparison. Molecular techniques and phylogenetic trees were adopted to detect X. euvesicatoria on P. pubescens using recQ, hrpB1, and hrpB2 genes for efficient and precise identification. For rapid molecular detection of X. euvesicatoria, loop-mediated isothermal amplification, polymerase chain reaction (PCR), and real-time PCR techniques were used. Whole genome comparison results showed that the genome of X. euvesicatoria was more closely relative to X. perforans than X. vesicatoria, and X. gardneri with 98%, 84%, and 86% ANI, respectively. All infected leaves of P. pubescens found positive amplification, and negative controls did not show amplification. The findings of evolutionary history revealed that isolated strains XeC10RQ, XeH9RQ, XeA10RQ, and XeB10RQ that originated from China were closely relative and highly homologous to the X. euvesicatoria. This research provides information to researchers on genomic variation in BLS pathogens, and further molecular evolution and identification of X. euvesicatoria using the unique target recQ gene through advance molecular approaches.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 2","pages":"191-206"},"PeriodicalIF":2.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ae/5b/ppj-oa-08-2022-0119.PMC10102564.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9307155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0