Plant Pathology Journal最新文献_第6页

A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of 'Candidatus Liberibacter asiaticus' in Citrus. 柑橘中5拷贝nrdB基因的实时环介导等温扩增检测

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.OA.02.2023.0030

Tirumalareddy Danda, Jong-Won Park, Kimberly L Timmons, Mamoudou Sétamou, Eliezer S Louzada, Madhurababu Kunta

{"title":"A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of 'Candidatus Liberibacter asiaticus' in Citrus.","authors":"Tirumalareddy Danda, Jong-Won Park, Kimberly L Timmons, Mamoudou Sétamou, Eliezer S Louzada, Madhurababu Kunta","doi":"10.5423/PPJ.OA.02.2023.0030","DOIUrl":"https://doi.org/10.5423/PPJ.OA.02.2023.0030","url":null,"abstract":"Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, 'Candidatus Liberibacter asiaticus' (CLas) is a non-culturable phloem-limited α-proteobacterium transmitted by Asian citrus psyllids (ACP, Diaphorina citri Kuwayama). A widely adopted method for HLB diagnosis is based on quantitative real-time polymerase chain reaction (qPCR). Although HLB diagnostic qPCR provides high sensitivity and good reproducibility, it is limited by time-consuming DNA preparation from plant tissue or ACP and the requirement of proper lab instruments including a thermal cycler to conduct qPCR. In an attempt to develop a quick assay that can be deployed in the field for CLas detection, we developed a real-time loop-mediated isothermal amplification (rt-LAMP) assay by targeting the CLas five copy nrdB gene. The rt-LAMP assay using various plant sample types and psyllids successfully detected the nrdB target as low as ~2.6 Log10 copies. Although the rt-LAMP assay was less sensitive than laboratory-based qPCR (detection limit ~10 copies), the data obtained with citrus leaf and bark and ACP showed that the rt-LAMP assay has >96% CLas detection rate, compared to that of laboratory-based qPCR. However, the CLas detection rate in fibrous roots was significantly decreased compared to qPCR due to low CLas titer in some root DNA sample. We also demonstrated that the rt-LAMP assay can be used with a crude leaf DNA extract which is fully deployable in the field for quick and reliable HLB screening.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"309-318"},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6f/36/ppj-oa-02-2023-0030.PMC10412965.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10349438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Endophytic Bacterial Communities and Investigation of Core Taxa in Apple Trees. 苹果内生细菌群落分析及核心类群调查。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.OA.05.2023.0070

Yejin Lee, Gyeongjun Cho, Da-Ran Kim, Youn-Sig Kwak

{"title":"Analysis of Endophytic Bacterial Communities and Investigation of Core Taxa in Apple Trees.","authors":"Yejin Lee, Gyeongjun Cho, Da-Ran Kim, Youn-Sig Kwak","doi":"10.5423/PPJ.OA.05.2023.0070","DOIUrl":"https://doi.org/10.5423/PPJ.OA.05.2023.0070","url":null,"abstract":"ire blight disease, caused by Erwinia amylovora, is a devastating affliction in apple cultivation worldwide. Chemical pesticides have exhibited limited effectiveness in controlling the disease, and biological control options for treating fruit trees are limited. Therefore, a relatively large-scale survey is necessary to develop microbial agents for apple trees. Here we collected healthy apple trees from across the country to identify common and core bacterial taxa. We analyzed the endophytic bacterial communities in leaves and twigs and discovered that the twig bacterial communities were more conserved than those in the leaves, regardless of the origin of the sample. This finding indicates that specific endophytic taxa are consistently present in healthy apple trees and may be involved in vital functions such as disease prevention and growth. Furthermore, we compared the community metabolite pathway expression rates of these endophyte communities with those of E. amylovora infected apple trees and discovered that the endophyte communities in healthy apple trees not only had similar community structures but also similar metabolite pathway expression rates. Additionally, Pseudomonas and Methylobacterium-Methylorobrum were the dominant taxa in all healthy apple trees. Our findings provide valuable insights into the potential roles of endophytes in healthy apple trees and inform the development of strategies for enhancing apple growth and resilience. Moreover, the similarity in cluster structure and pathway analysis between healthy orchards was mutually reinforcing, demonstrating the power of microbiome analysis as a tool for identifying factors that contribute to plant health.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"397-408"},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/42/06/ppj-oa-05-2023-0070.PMC10412964.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10349441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Resistance of Diaporthe citri against Systemic Fungicides on Citrus. 柑桔对系统杀菌剂的化学抗性。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.OA.05.2023.0069

Zar Zar Soe, Yong Ho Shin, Hyun Su Kang, Yong Chull Jeun

{"title":"Chemical Resistance of Diaporthe citri against Systemic Fungicides on Citrus.","authors":"Zar Zar Soe, Yong Ho Shin, Hyun Su Kang, Yong Chull Jeun","doi":"10.5423/PPJ.OA.05.2023.0069","DOIUrl":"https://doi.org/10.5423/PPJ.OA.05.2023.0069","url":null,"abstract":"Citrus melanose, caused by Diaporthe citri, has been one of the serious diseases, and chemical fungicides were used for protection in many citrus orchards of Jeju Island. Establishing a disinfectant resistance management system and reducing pesticide usage would be important for contributing to safe agricultural production. In this study, monitoring of chemical resistance was performed with 40 representative D. citri isolates from many citrus orchards in Jeju Island. Four different fungicides, kresoxim-methyl, benomyl, fluazinam, and prochloraz manganese, with seven different concentrations were tested in vitro by growing the mycelium of the fungal isolates on the artificial medium potato dextrose agar. Among the 40 fungal isolates, 12 isolates were investigated as resistant to kresoxim-methyl which could not inhibit the mycelium growth to more than 50%. Especially isolate NEL21-2 was also resistant against benomyl, whose hyphae grew well even on the highest chemical concentration. However, any chemical resistance of fungal isolates was found against neither fluazinam nor prochloraz manganese. On the other hand, in vivo bio-testing of some resistant isolates was performed against both kresoxim-methyl and benomyl on young citrus leaves. Typical melanose symptoms developed on the citrus leaves pre-treated with both agrochemicals after inoculation with the resistant isolates. However, no or less symptoms were observed when the susceptible isolates were inoculated. Based on these results, it was suggested that some resistant isolates of D. citri occurred against both systemic fungicides, which may be valuable to build a strategy for protecting citrus disease.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"351-360"},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7f/67/ppj-oa-05-2023-0069.PMC10412969.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10349437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Analysis of the Carrot Bacterial Blight Pathogen Xanthomonas hortorum pv. carotae in Korea. 韩国胡萝卜细菌性疫病病原体黄单胞菌（Xanthomonas hortorum pv. carotae）的基因组分析。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.NT.11.2022.0149

Mi-Hyun Lee, Sung-Jun Hong, Dong Suk Park, Hyeonheui Ham, Hyun Gi Kong

{"title":"Genomic Analysis of the Carrot Bacterial Blight Pathogen Xanthomonas hortorum pv. carotae in Korea.","authors":"Mi-Hyun Lee, Sung-Jun Hong, Dong Suk Park, Hyeonheui Ham, Hyun Gi Kong","doi":"10.5423/PPJ.NT.11.2022.0149","DOIUrl":"10.5423/PPJ.NT.11.2022.0149","url":null,"abstract":"Bacterial leaf blight of carrots caused by Xanthomonas hortorum pv. carotae (Xhc) is an important worldwide seed-borne disease. In 2012 and 2013, symptoms similar to bacterial leaf blight were found in carrot farms in Jeju Island, Korea. The phenotypic characteristics of the Korean isolation strains were similar to the type strain of Xhc. Pathogenicity showed symptoms on the 14th day after inoculation on carrot plants. Identification by genetic method was multi-position sequencing of the isolated strain JJ2001 was performed using four genes (danK, gyrB, fyuA, and rpoD). The isolated strain was confirmed to be most similar to Xhc M081. Furthermore, in order to analyze the genetic characteristics of the isolated strain, whole genome analysis was performed through the next-generation sequencing method. The draft genome size of JJ2001 is 5,443,372 bp, which contains 63.57% of G + C and has 4,547 open reading frames. Specifically, the classification of pathovar can be confirmed to be similar to that of the host lineage. Plant pathogenic factors and determinants of the majority of the secretion system are conserved in strain JJ2001. This genetic information enables detailed comparative analysis in the pathovar stage of pathogenic bacteria. Furthermore, these findings provide basic data for the distribution and diagnosis of Xanthomonas hortorum pv. carotae, a major plant pathogen that infects carrots in Korea.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"409-416"},"PeriodicalIF":1.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/15/ppj-nt-11-2022-0149.PMC10412968.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10330663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Weeds around Capsicum annuum (CA) Cultivation Fields as Potential Habitats of CA-Infecting Viruses. 辣椒田杂草作为CA侵染病毒潜在生境的评价

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.OA.04.2023.0066

Min-Kyung Choi

{"title":"Evaluation of the Weeds around Capsicum annuum (CA) Cultivation Fields as Potential Habitats of CA-Infecting Viruses.","authors":"Min-Kyung Choi","doi":"10.5423/PPJ.OA.04.2023.0066","DOIUrl":"https://doi.org/10.5423/PPJ.OA.04.2023.0066","url":null,"abstract":"Capsicum annuum (CA) is grown outdoors across fields in Jeollabuk-do, South Korea. The weeds surrounding these fields were investigated regarding the infection of 11 viruses infecting CA during the year 2014-2018. In the reverse transcription polymerase chain reaction diagnosis, 546 out of 821 CA samples (66.5%) were infected by nine viruses, and 190 out of 918 weed samples (20.7%) were infected by eight viruses. Correlation analysis of the mutual influence of the viruses infecting CA and weeds during these 5 years showed that five viruses had significant positive correlations with the infection in both CA and weeds. Over the study period, the weeds infected by cucumber mosaic virus (CMV) in the previous year were positively correlated with the incidence of CMV infection in CA in the current year, although the correlation was lower for tomato spotted wilt virus (TSWV) compared to CMV. The CMV infection percent was 14.0% in summer annuals, 11.4% in perennials, and 7.8% in winter annuals. However, considering the overwintering period without CA, the infection percent was 5.2% higher in winter annuals and perennials than that in summer annuals, indicating that winter annual and perennial weeds served as the main habitats for insect vectors. The TSWV infection percent in weeds was 10.4% in summer annuals, 6.4% in winter annuals, and 6.2% in perennials. The weeds surrounding CA fields, acting as the intermediate hosts, were found to be the potent sources of infection, influencing the spread and diversity of CA-infecting viruses. The results of this study can contribute to prevent viral infection in agricultural fields.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"374-383"},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e2/4c/ppj-oa-04-2023-0066.PMC10412962.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9977598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Analysis of Plant Diseases Identification Based on Deep Learning Methods. 基于深度学习方法的植物病害识别分析

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.OA.02.2023.0034

Xulu Gong, Shujuan Zhang

{"title":"An Analysis of Plant Diseases Identification Based on Deep Learning Methods.","authors":"Xulu Gong, Shujuan Zhang","doi":"10.5423/PPJ.OA.02.2023.0034","DOIUrl":"https://doi.org/10.5423/PPJ.OA.02.2023.0034","url":null,"abstract":"Plant disease is an important factor affecting crop yield. With various types and complex conditions, plant diseases cause serious economic losses, as well as modern agriculture constraints. Hence, rapid, accurate, and early identification of crop diseases is of great significance. Recent developments in deep learning, especially convolutional neural network (CNN), have shown impressive performance in plant disease classification. However, most of the existing datasets for plant disease classification are a single background environment rather than a real field environment. In addition, the classification can only obtain the category of a single disease and fail to obtain the location of multiple different diseases, which limits the practical application. Therefore, the object detection method based on CNN can overcome these shortcomings and has broad application prospects. In this study, an annotated apple leaf disease dataset in a real field environment was first constructed to compensate for the lack of existing datasets. Moreover, the Faster R-CNN and YOLOv3 architectures were trained to detect apple leaf diseases in our dataset. Finally, comparative experiments were conducted and a variety of evaluation indicators were analyzed. The experimental results demonstrate that deep learning algorithms represented by YOLOv3 and Faster R-CNN are feasible for plant disease detection and have their own strong points and weaknesses.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"319-334"},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/13/7d/ppj-oa-02-2023-0034.PMC10412967.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9977596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

MoJMJD6, a Nuclear Protein, Regulates Conidial Germination and Appressorium Formation at the Early Stage of Pathogenesis in Magnaporthe oryzae. MoJMJD6是一种核蛋白，在水稻大孔霉发病早期调控分生孢子萌发和附着胞形成。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.OA.12.2022.0161

Li Zhang, Dong Li, Min Lu, Zechi Wu, Chaotian Liu, Yingying Shi, Mengyu Zhang, Zhangjie Nan, Weixiang Wang

{"title":"MoJMJD6, a Nuclear Protein, Regulates Conidial Germination and Appressorium Formation at the Early Stage of Pathogenesis in Magnaporthe oryzae.","authors":"Li Zhang, Dong Li, Min Lu, Zechi Wu, Chaotian Liu, Yingying Shi, Mengyu Zhang, Zhangjie Nan, Weixiang Wang","doi":"10.5423/PPJ.OA.12.2022.0161","DOIUrl":"https://doi.org/10.5423/PPJ.OA.12.2022.0161","url":null,"abstract":"In plant-pathogen interactions, Magnaporthe oryzae causes blast disease on more than 50 species of 14 monocot plants, including important crops such as rice, millet, and most 15 recently wheat. M. oryzae is a model fungus for studying plant-microbe interaction, and the main source for fungal pathogenesis in the field. Here we report that MoJMJD6 is required for conidium germination and appressorium formation in M. oryzae. We obtained MoJMJD6 mutants (ΔMojmjd6) using a target gene replacement strategy. The MoJMD6 deletion mutants were delayed for conidium germination, glycogen, and lipid droplets utilization and consequently had decreased virulence. In the ΔMojmjd6 null mutants, global histone methyltransferase modifications (H3K4me3, H3K9me3, H3K27me3, and H3K36me2/3) of the genome were unaffected. Taken together, our results indicated that MoJMJD6 function as a nuclear protein which plays an important role in conidium germination and appressorium formation in the M. oryzae. Our work provides insights into MoJMJD6-mediated regulation in the early stage of pathogenesis in plant fungi.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"361-373"},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9f/80/ppj-oa-12-2022-0161.PMC10412966.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9977597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-identification of Colletotrichum acutatum Species Complex in Korea and Their Host Plants. 重新鉴定韩国的 Colletotrichum acutatum 复合菌种及其寄主植物。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.OA.05.2023.0078

Le Dinh Thao, Hyorim Choi, Yunhee Choi, Anbazhagan Mageswari, Daseul Lee, Seung-Beom Hong

{"title":"Re-identification of Colletotrichum acutatum Species Complex in Korea and Their Host Plants.","authors":"Le Dinh Thao, Hyorim Choi, Yunhee Choi, Anbazhagan Mageswari, Daseul Lee, Seung-Beom Hong","doi":"10.5423/PPJ.OA.05.2023.0078","DOIUrl":"10.5423/PPJ.OA.05.2023.0078","url":null,"abstract":"Colletotrichum acutatum species complex is one of the most important groups in the genus Colletotrichum with a high species diversity and a wide range of host plants. C. acutatum and related species have been collected from different plants and locations in Korea and deposited into the Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Sciences since the 1990s. These fungal isolates were previously identified based mainly on morphological characteristics, and a limitation of molecular data was provided. To confirm the identification of species, 64 C. acutatum species complex isolates in KACC were used in this study for DNA sequence analyses of six loci: nuclear ribosomal internal transcribed spacers (ITS), betatubulin 2 (TUB2), histone-3 (HIS3), glyceraldehyde3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and actin (ACT). The molecular analysis revealed that they were identified in six different species of C. fioriniae (24 isolates), C. nymphaeae (21 isolates), C. scovillei (12 isolates), C. chrysanthemi (three isolates), C. lupini (two isolates), and C. godetiae (one isolate), and a novel species candidate. We compared the hosts of KACC isolates with \"The List of Plant Diseases in Korea\", previous reports in Korea and global reports and found that 23 combinations between hosts and pathogens could be newly reported in Korea after pathogenicity tests, and 12 of these have not been recorded in the world.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"384-396"},"PeriodicalIF":1.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/01/ppj-oa-05-2023-0078.PMC10412970.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10349434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of Faba Bean Diseases and Determinants of Faba Bean Gall (Physoderma sp.) Epidemics in Ethiopia. 埃塞俄比亚蚕豆病的发生及蚕豆瘿病流行的决定因素

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-08-01 DOI: 10.5423/PPJ.OA.03.2023.0040

Tekalign Zeleke, Bereket Ali, Asenakech Tekalign, Gudisa Hailu, M J Barbetti, Alemayehu Ayele, Tajudin Aliyi, Alemu Ayele, Abadi Kahsay, Belachew Tiruneh, Fekadu Tewolde

{"title":"Occurrence of Faba Bean Diseases and Determinants of Faba Bean Gall (Physoderma sp.) Epidemics in Ethiopia.","authors":"Tekalign Zeleke, Bereket Ali, Asenakech Tekalign, Gudisa Hailu, M J Barbetti, Alemayehu Ayele, Tajudin Aliyi, Alemu Ayele, Abadi Kahsay, Belachew Tiruneh, Fekadu Tewolde","doi":"10.5423/PPJ.OA.03.2023.0040","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2023.0040","url":null,"abstract":"Physoderma fungal species cause faba bean gall (FBG) which devastates faba bean (Vicia faba L.) in the Ethiopian highlands. In three regions (Amahara, Oromia, and Tigray), the relative importance, distribution, intensity, and association with factors affecting FBG damage were assessed for the 2019 (283 fields) and 2020 (716 fields) main cropping seasons. A logistic regression model was used to associate biophysical factors with FBG incidence and severity. Amhara region has the highest prevalence of FBG (95.7%), followed by Tigray (83.3%), and the Oromia region (54%). Maximum FBG incidence (78.1%) and severity (32.8%) were recorded from Amhara and Tigray areas, respectively. The chocolate spot was most prevalent in West Shewa, Finfinne Special Zone, and North Shewa of the Oromia region. Ascochyta blight was found prevalent in North Shewa, West Shewa, Southwest Shewa of Oromia, and the South Gondar of Amhara. Faba bean rust was detected in all zones except for the South Gonder and North Shewa, and root rot disease was detected in all zones except South Gonder, South Wollo, and North Shewa of Amahara. Crop growth stage, cropping system, altitude, weed density, and fungicide, were all found to affect the incidence and severity of the FBG. Podding and maturity stage, mono-cropping, altitude (>2,400), high weed density, and non-fungicide were found associated with increased disease intensities. However, crop rotation, low weed infestation, and fungicide usage were identified as potential management options to reduce FBG disease.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 4","pages":"335-350"},"PeriodicalIF":2.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/28/2a/ppj-oa-03-2023-0040.PMC10412963.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10349436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations in Kiwifruit Microbiota across Cultivars and Tissues during Developmental Stages. 猕猴桃发育阶段不同品种和组织微生物群的变化。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-06-01 DOI: 10.5423/PPJ.OA.03.2023.0038

Su-Hyeon Kim, Da-Ran Kim, Youn-Sig Kwak

{"title":"Variations in Kiwifruit Microbiota across Cultivars and Tissues during Developmental Stages.","authors":"Su-Hyeon Kim, Da-Ran Kim, Youn-Sig Kwak","doi":"10.5423/PPJ.OA.03.2023.0038","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2023.0038","url":null,"abstract":"The plant microbiota plays a crucial role in promoting plant health by facilitating the nutrient acquisition, abiotic stress tolerance, biotic stress resilience, and host immune regulation. Despite decades of research efforts, the precise relationship and function between plants and microorganisms remain unclear. Kiwifruit (Actinidia spp.) is a widely cultivated horticultural crop known for its high vitamin C, potassium, and phytochemical content. In this study, we investigated the microbial communities of kiwifruit across different cultivars (cvs. Deliwoong and Sweetgold) and tissues at various developmental stages. Our results showed that the microbiota community similarity was confirmed between the cultivars using principal coordinates analysis. Network analysis using both degree and eigenvector centrality indicated similar network forms between the cultivars. Furthermore, Streptomycetaceae was identified in the endosphere of cv. Deliwoong by analyzing amplicon sequence variants corresponding to tissues with an eigenvector centrality value of 0.6 or higher. Our findings provide a foundation for maintaining kiwifruit health through the analysis of its microbial community.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 3","pages":"245-254"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/10/9f/ppj-oa-03-2023-0038.PMC10265115.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9994497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0