Plant Pathology Journal最新文献_第6页

Genetic Diversity and Genotype Distribution of Erwinia amylovora in Korea. 韩国鹿角菌的遗传多样性和基因型分布。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-02-01 DOI: 10.5423/PPJ.OA.09.2024.0151

Hyeonheui Ham, Se-Weon Lee, Yong Hwan Lee

{"title":"Genetic Diversity and Genotype Distribution of Erwinia amylovora in Korea.","authors":"Hyeonheui Ham, Se-Weon Lee, Yong Hwan Lee","doi":"10.5423/PPJ.OA.09.2024.0151","DOIUrl":"10.5423/PPJ.OA.09.2024.0151","url":null,"abstract":"Erwinia amylovora, first identified in 1793 in Hudson Valley (New York, USA), has a genome size of 3.7-4.0 Mb. E. amylovora bacterial strains are classified based on the infecting hosts: the Amygdaloideae-infecting (AI) group, targeting apple and pear trees, and the Rubus-infecting group, affecting berry trees. Since the AI-group strains display high genetic similarity (˃99.7%), it is challenging to characterize their genotypes. This study investigated the genetic diversity of E. amylovora isolates in Korea and the regional distribution patterns of genotypes using a multilocus variable number of tandem repeat analysis (MLVA). Four specific primers were used to amplify and sequence tandem repeats in the E. amylovora genome, and a distribution map of E. amylovora was created using MLVA genotypes. Thirty-two types of MLVA patterns were identified in Korean strains, and RV19 was the dominant type identified in all South Korean regions. According to the minimal spanning tree, genotypes were differentiated into RV7, RV14, RV20, RV22, and RV27 types, originating from the RV19 type. This finding suggests that the RV19 type, introduced to Korea for the first time, spread to other regions from Anseong-si, Cheonan-si, Chungju-si, and Jecheon-si, depending on the type. We determined the MLVA genotypes of E. amylovora isolates and distribution patterns by region from 2019 to 2023. The distribution of these genotypes by year and region provides basic information for the genetic diversity and spread of E. amylovora in Korea.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 1","pages":"88-99"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Temperature on Resistance to Streptomycin in Xanthomonas arboricola pv. pruni. 温度对树黄单胞菌对链霉素抗性的影响。pruni。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-02-01 DOI: 10.5423/PPJ.OA.08.2024.0119

Ji Ho Song, Hyun Gi Kong

{"title":"Effects of Temperature on Resistance to Streptomycin in Xanthomonas arboricola pv. pruni.","authors":"Ji Ho Song, Hyun Gi Kong","doi":"10.5423/PPJ.OA.08.2024.0119","DOIUrl":"10.5423/PPJ.OA.08.2024.0119","url":null,"abstract":"Xanthomonas arboricola pv. pruni (Xap) causes the shot hole disease of stone fruits and almonds. This bacterium is a damaging, widespread pathogen distributed across the major stone fruit producing regions of the world. To control shot hole disease, antibiotics such as streptomycin are mainly used. However, as concerns about antibiotic resistance increase, many restrictions are placed on the use of antibiotics. Additionally, it has been reported that the rise in temperature due to climate change affects disease occurrence and ecology. Therefore, in this study, we determined the minimum inhibitory concentration (MIC) of streptomycin for Xap at an optimal growth temperature of 28°C and investigated the changes in MIC and the occurrence frequency of resistant bacteria at 10°C, 25°C and 30°C. The results of this study showed that the MIC was 30 µg/ml at 28°C. In addition, when the change in streptomycin resistance concentration due to temperature was confirmed, we found that the resistance concentration decreased to 10 µg/ml at 30°C. When the occurrence of resistance according to concentration and temperature conditions was investigated, the occurrence frequency of resistant strains was found to be the highest at 50 µg/ml. In the case of temperature, the occurrence frequency of resistant strains was confirmed to be high at 30°C. These results provide basic data for further reducing the problem of antibiotic resistance by suggesting the possibility of changes in the occurrence of streptomycin-resistant strains depending on the antibiotic treatment environment.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 1","pages":"78-87"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory Effects of Garlic Extract on Hop Stunt Viroid in Micropropagated Grapevine Plantlets. 大蒜提取物对葡萄小苗啤酒花矮病毒的抑制作用。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-02-01 DOI: 10.5423/PPJ.OA.10.2024.0169

Chae-Min Kang, Rae-Dong Jeong

{"title":"Inhibitory Effects of Garlic Extract on Hop Stunt Viroid in Micropropagated Grapevine Plantlets.","authors":"Chae-Min Kang, Rae-Dong Jeong","doi":"10.5423/PPJ.OA.10.2024.0169","DOIUrl":"10.5423/PPJ.OA.10.2024.0169","url":null,"abstract":"Hop stunt viroid (HSVd) is a major pathogen that affects grapevine health and causes substantial economic losses in grape cultivation. Many studies have been conducted to control grapevine diseases, but effective control methods after plant infections remain lacking. This study aimed to assess the antiviral potential of garlic extract, a natural substance that inhibits HSVds in grapevine plants after micropropagation. Garlic extract was diluted 1,000-fold and applied to grapevine plants, and its effect on HSVd accumulation was evaluated using reverse transcription polymerase chain reaction and digital PCR. The results showed that HSVd accumulation was significantly reduced, with an inhibition rate of 74.45%; meanwhile, higher garlic extract concentrations resulted in contamination and plant damage. Nanopore sequencing confirmed that the integrity of the HSVd genome was compromised after treatment. Furthermore, garlic extract inhibited the HSVd and promoted plant growth by enhancing shoot and root development. Additionally, inhibition of the HSVd was sustained in regenerated grapevine plants. Moreover, the garlic extract showed inhibitory effects against HSVds in natural host cucumber plants. These results suggest that garlic extract could be a cost-effective and sustainable alternative for viroid control in grapevine cultivation, providing long-term protection and broader antiviral activity across plant species.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 1","pages":"51-63"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Insidious Threat: Assessing the Dangers and Spread of Tomato Leaf Curl New Delhi Virus. 潜伏的威胁：评估番茄卷曲新德里病毒的危险和传播。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-02-01 DOI: 10.5423/PPJ.RW.11.2024.0177

Thuy Thi Bich Vo, Marjia Tabassum, Bupi Nattanong, Muhammad Amir Qureshi, Hyo-Jin Im, Giuseppe Parrella, Eui-Joon Kil, Sukchan Lee

{"title":"The Insidious Threat: Assessing the Dangers and Spread of Tomato Leaf Curl New Delhi Virus.","authors":"Thuy Thi Bich Vo, Marjia Tabassum, Bupi Nattanong, Muhammad Amir Qureshi, Hyo-Jin Im, Giuseppe Parrella, Eui-Joon Kil, Sukchan Lee","doi":"10.5423/PPJ.RW.11.2024.0177","DOIUrl":"10.5423/PPJ.RW.11.2024.0177","url":null,"abstract":"Begomoviruses have significantly threatened many important crops worldwide, causing substantial issues for years. New viruses from this genus are frequently identified, displaying increasingly harmful traits. Among the numerous species within this group, Tomato leaf curl New Delhi virus (ToLCNDV) stands out as a particularly dangerous member due to its notable pathogenic characteristics. This virus poses a serious threat to crops, leading to considerable economic losses. Although ToLCNDV has not been detected in Korea, there is no definitive assurance that it will not appear in the future. Thus, understanding the features and mechanisms of this virus, alongside extensive research on ToLCNDV characteristics and the development of effective preventive strategies, is essential. This review underscores key aspects of ToLCNDV, stressing the risks this virus poses to agriculture. Furthermore, recent advances in breeding natural resistance in key crops are discussed, offering a foundation for improved control methods and preparedness in regions currently unaffected, such as Korea, to mitigate potential agricultural impacts should the virus emerge.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 1","pages":"1-16"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Incidence and Genetic Analysis of Two Betaflexiviruses Capillovirus alphavii and Tepovirus tafpruni in Iran. 两种倍他曲病毒在伊朗的发病率和遗传分析。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-02-01 DOI: 10.5423/PPJ.OA.10.2024.0158

Reza Pourrrahim, Shirin Farzadfar

{"title":"The Incidence and Genetic Analysis of Two Betaflexiviruses Capillovirus alphavii and Tepovirus tafpruni in Iran.","authors":"Reza Pourrrahim, Shirin Farzadfar","doi":"10.5423/PPJ.OA.10.2024.0158","DOIUrl":"10.5423/PPJ.OA.10.2024.0158","url":null,"abstract":"Viral diseases have emerged as a serious threat to cherry trees production in Iran. To determine which virus(es) are present, three leaves from a sweet cherry tree (Qz5) with diffuse white blotch spots and deformation were subjected to high-throughput sequencing. After de novo assembly, blast analysis revealed that 12 contigs ranging from 360 to 7,433 nucleotides (nts) shared 78-96% nt identities with Capillovirus alphavii (cherry virus A, CVA) and seven contigs, ranging from 350 to 6,844 nts, shared 79-88% nt identities with Tepovirus tafpruni (prunus virus T, PrVT). During a survey, CVA, PrVT, and CVA + PrVT infections were detected in 12.6%, 5.1%, and 7.9% of 724 sour and sweet cherry samples. Phylogenetic analysis revealed that Iranian CVA was grouped into GIIIB, whereas PrVT fell into a distinct branch, which was confirmed by diversity plots. The within-population diversity was lower than the between-population diversity suggesting the contribution of a founder effect on diversification of CVA isolates. Host-specific codon adaptation analysis revealed the highest adaptation of CVA to sour cherry. This could suggest that sour cherry may be one of the closest Prunus species to wild progenitors. It raises the possibility that viruses such as CVA may have exerted evolutionary pressures influencing domestication processes. Additionally, the similarity index indicated that the common plum (Prunus domestica) may have exerted significant evolutionary pressure on CVA and PrVT. The association of CVA and PrVT was reported for the first time in the mid-Eurasian region, specifically in Iran, which represents an issue in phytosanitary certification of cherry plants.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 1","pages":"38-50"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput Screening of Plant Extracts for Targeted Control of Burkholderia glumae, Causing Rice Sheath and Panicle Blight. 靶向防治水稻叶枯病的植物提取物的高通量筛选

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-02-01 DOI: 10.5423/PPJ.NT.10.2024.0167

Seungchul Lee, Yong Tae Jeong, Seokhun Jang, Taeho Jung, Buyng Su Hwang, Ji Su Choi, Young Taek Oh, Mohamed Mannaa, Young-Su Seo

{"title":"High-Throughput Screening of Plant Extracts for Targeted Control of Burkholderia glumae, Causing Rice Sheath and Panicle Blight.","authors":"Seungchul Lee, Yong Tae Jeong, Seokhun Jang, Taeho Jung, Buyng Su Hwang, Ji Su Choi, Young Taek Oh, Mohamed Mannaa, Young-Su Seo","doi":"10.5423/PPJ.NT.10.2024.0167","DOIUrl":"10.5423/PPJ.NT.10.2024.0167","url":null,"abstract":"Rice (Oryza sativa), a staple crop worldwide, is severely threatened by bacterial panicle blight caused by Burkholderia glumae, leading to substantial yield losses. The lack of effective chemical treatments and resistant rice cultivars highlights the urgent need for alternative solutions. In this study, 1,134 plant extracts were screened for antibacterial activity against B. glumae using agar disc diffusion and liquid broth assays. Thirty-three extracts exhibited significant growth inhibition on agar plates. These 33 extracts were further tested in Luria- Bertani broth, where five showed notable activity, and two extracts-Trapa japonica (FBCC-EP312) and Rumex crispus (FBCC-EP487)-were selected for detailed analysis. Both extracts significantly reduced bacterial motility and disease severity in rice, while having no effect on non-target bacteria such as Escherichia coli. These findings highlight the potential of these plant-derived compounds as effective biocontrol agents, offering an eco-friendly alternative to synthetic pesticides and promising applications in sustainable agriculture.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 1","pages":"112-119"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity and Population Structure of Phyllosphere-Associated Xanthomonas euvesicatoria Bacteria in Physalis pubescens Based on BOX-PCR and ERIC-PCR in China. 基于BOX-PCR和ERIC-PCR的中国短毛Physalis Phyllosphere-Associated euvesicatoria黄单胞菌的遗传多样性和群体结构

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-02-01 DOI: 10.5423/PPJ.OA.09.2024.0138

Faisal Siddique, Xu Xiaofeng, Ni Zhe, Yang Mingxiu, Liu Dawei, Li Yuting, Yang Naibo, Haseeb Younis, Nihal Niaz, Zhang Junhua

{"title":"Genetic Diversity and Population Structure of Phyllosphere-Associated Xanthomonas euvesicatoria Bacteria in Physalis pubescens Based on BOX-PCR and ERIC-PCR in China.","authors":"Faisal Siddique, Xu Xiaofeng, Ni Zhe, Yang Mingxiu, Liu Dawei, Li Yuting, Yang Naibo, Haseeb Younis, Nihal Niaz, Zhang Junhua","doi":"10.5423/PPJ.OA.09.2024.0138","DOIUrl":"10.5423/PPJ.OA.09.2024.0138","url":null,"abstract":"Xanthomonas euvesicatoria has become a serious problem in Physalis pubescens, leading to substantial crop losses. In our previous investigation, we used rapid molecular detection techniques to identify X. euvesicatoria; however, this pathogen's diversity and population structure remain poorly understood, despite their importance in disease management. To address this knowledge gap, we analyzed the diversity of X. euvesicatoria using BOX-PCR and ERIC-PCR fingerprinting techniques. A total of 103 isolates were collected from 13 counties across Heilongjiang province during the 2018 and 2019 growing seasons. Our findings revealed 635 unique genetic patterns from ERIC-PCR fingerprinting, compared to 360 patterns from BOX-PCR. BOX-PCR analysis identified 12 distinct genotypic clusters, whereas ERIC-PCR identified 14 clusters through unweighted pair group approach with arithmetic average analysis, demonstrating substantial genetic variability. STRUCTURE analysis further identified five distinct genetic clusters in the BOX-PCR data and two in the ERIC-PCR data. The Hailin isolates showed the highest level of diversification compared to other regional isolates. AMOVA results indicated that 85% of the genetic variation in BOX-PCR was attributable to within-population differences, while 78% of ERIC-PCR variation was due to differences across populations. In addition, a Mantel test demonstrated a tenuous correlation between BOX-PCR and ERIC-PCR genetic markers, indicating distinct genetic profiles. This extensive genetic information enhances our understanding of the epidemiology of bacterial leaf spot and its potential therapeutic prospects. These data can provide insights into Xanthomonas strains' diversity and geographical dissemination.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 1","pages":"64-77"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Analysis of KPvRxLR27, a Novel Plasmopara viticola Effector from a Korean Isolate, and Its Role in Hypersensitive Response. 韩国葡萄浆原体效应蛋白KPvRxLR27的功能分析及其在过敏反应中的作用

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2025-02-01 Epub Date: 2024-12-16 DOI: 10.5423/PPJ.OA.09.2024.0141

Marc Semunyana, Rahel Dinsa Guta, Guogeng Jia, Soomin Lee, Inyong Jeong, Khaing Shwe Zin Thinn, DoYoon Lim, Siyeon Lim, Bomi Lee, HyunJu Kim, May Moe Oo, Sun Ha Kim, Jiyoung Min, Sang-Keun Oh

{"title":"Functional Analysis of KPvRxLR27, a Novel Plasmopara viticola Effector from a Korean Isolate, and Its Role in Hypersensitive Response.","authors":"Marc Semunyana, Rahel Dinsa Guta, Guogeng Jia, Soomin Lee, Inyong Jeong, Khaing Shwe Zin Thinn, DoYoon Lim, Siyeon Lim, Bomi Lee, HyunJu Kim, May Moe Oo, Sun Ha Kim, Jiyoung Min, Sang-Keun Oh","doi":"10.5423/PPJ.OA.09.2024.0141","DOIUrl":"10.5423/PPJ.OA.09.2024.0141","url":null,"abstract":"Plasmopara viticola causes grape downy mildew, one of the most notorious diseases of cultivated grapes that damage vineyards worldwide. The pathogen secretes various effector molecules to infect and modulate the host biological processes. In this study, we aimed to evaluate the roles of KPvRxLR27, an arginine-any amino acid-leucine-arginine (RxLR) effector isolated from P. viticola JN-9 from Jeonju (South Korea) with respect to the reported Bcl-2-associated X and inverted formin1in inducing cell death in non-host Nicotiana benthamiana and resistant grape host cultivars via Agrobacterium-mediated transient transformation. We found that, KPvRxLR27 induced programmed cell death in N. benthamiana and rapid hypersensitive response in resistant grape cultivars. Agroinfiltration assay revealed that putative N-glycosylation at the N186 amino acid sequence and nuclear localization signal motifs at the C-terminus were critical for the effector's cell death-inducing activity of KPvRxLR27. Overexpression assay revealed that KPvRxLR27 was abundantly expressed in the plasma membrane and nuclear regions and activated the accumulation of reactive oxygen species in N. benthamiana. Moreover, KPvRxLR27 expression was significantly delayed in the resistant cultivar than in the susceptible cultivar. Our results suggest KPvRxLR27 as a potential avirulence gene recognized by the host receptors to activate the host immune response-associated genes, providing valuable insights to enhance the pathogen resistance of commercial cultivars.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":" ","pages":"28-37"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protection Efficacy of Antibacterial Strains against Fire Blight Caused by Erwinia amylovora on Apple Blossom. 抑菌菌株对苹果花火枯病的保护效果研究。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.09.2024.0148

Sujeong Kim, Yong Ho Shin, Sonja Weißhaupt, Stefan Kunz, Yong Chull Jeun

{"title":"Protection Efficacy of Antibacterial Strains against Fire Blight Caused by Erwinia amylovora on Apple Blossom.","authors":"Sujeong Kim, Yong Ho Shin, Sonja Weißhaupt, Stefan Kunz, Yong Chull Jeun","doi":"10.5423/PPJ.OA.09.2024.0148","DOIUrl":"10.5423/PPJ.OA.09.2024.0148","url":null,"abstract":"Fire blight caused by Erwinia amylovora is one of the destructive diseases in the family of Rosaceae plants, including apple and pear, in the world. Since the first report in 2015, the number of infected farms and area steadily increased in Korea. In case of eradication failure against this disease, protection strategies using both chemicals and biocontrol agents should be established. In this study, to select an effective antibacterial agent against fire blight on apple trees, four bacterial strains isolated from Jeju Island were investigated. Among the bacterial strains, Bacillus circulans BRH433-2 showed bactericidal effects against E. amylovora Ea385 forming inhibition zone on an artificial medium. The other bacterial strains such as Pseudomonas fluorescens THJ609-3, Micrococcus luteus TRK2-2 and P. fluorescens TRH415-2 showed bacteriostatic activity preventing growth of E. amylovora Ea385 in shaken cultures as well as on detached apple blossoms inoculated with E. amylovora Ea385, as measured with quantitative PCR. Bio-tests on detached blossoms showed that the treatment with all bacterial strains caused strong suppression of bacterial ooze formation, indicating inhibition of disease incidence of fire blight, which was similar to blossoms treated with streptomycin sulfate. Therefore, it was suggested that these bacterial strains may be useful in organic apple orchards to control fire blight where chemical use is limited.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"633-640"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and Characterization of a Full-Length Infectious cDNA Clone of a Strain of Watermelon Mosaic Virus Isolated from Melon. 西瓜花叶病毒全长感染性cDNA克隆的构建与鉴定。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-12-01 DOI: 10.5423/PPJ.OA.07.2024.0103

Hae-Ryun Kwak, Su-Bin Hong, Jeong-Eun Kim, Hee-Seong Byun, Bong-Choon Lee, Hong-Soo Choi, Mikyeong Kim

{"title":"Construction and Characterization of a Full-Length Infectious cDNA Clone of a Strain of Watermelon Mosaic Virus Isolated from Melon.","authors":"Hae-Ryun Kwak, Su-Bin Hong, Jeong-Eun Kim, Hee-Seong Byun, Bong-Choon Lee, Hong-Soo Choi, Mikyeong Kim","doi":"10.5423/PPJ.OA.07.2024.0103","DOIUrl":"10.5423/PPJ.OA.07.2024.0103","url":null,"abstract":"Watermelon mosaic virus (WMV), a member of the genus Potyvirus, causes serious economic losses in cucurbit crops. For molecular biological studies of viruses, it is necessary to construct an infectious clone that can facilitate gene functional analysis. In this study, we constructed an infectious cDNA clone of WMV genomic RNA by Gibson assembly and evaluated its virulence and symptoms on a variety of host plants. A WMV isolate (WMV-IS-me2) collected from melon in Iksan, Jeonbuk Province, Korea in 2015 caused mosaic symptoms on leaves. Four overlapping PCR fragments of the full-length genome of this isolate and the pJL89 binary vector with overhanging ends of 40 bp were amplified by reverse transcription PCR and joined in a single isothermal reaction. The complete nucleotide sequence of the infectious cDNA clone of WMV was determined and the recombinant vector was transformed into Agrobacterium tumefaciens GV3101. Following agro-infiltration, an infectious clone (pWMV-M, KACC95145P) systemically induced mosaic symptoms on watermelon (Citrullus lanatus), zucchini (Cucurbita pepo), melon (Cucumis melo), and Nicotiana benthamiana plants. This infectious clone may be useful for screening cucurbit varieties resistant to WMV, as well as for studying viral gene function.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 6","pages":"615-624"},"PeriodicalIF":1.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0