Plant Pathology Journal最新文献_第5页

Stem Rot of Pearl Millet Prevalence, Symptomatology, Disease Cycle, Disease Rating Scale and Pathogen Characterization in Pearl Millet-Klebsiella Pathosystem. 珍珠米-克雷伯氏菌病原系统中珍珠米茎腐病的发病率、症状、病害周期、病害量表和病原特征。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.09.2023.0126

Vinod Kumar Malik, Pooja Sangwan, Manjeet Singh, Pavitra Kumari, Niharika Shoeran, Navjeet Ahalawat, Mukesh Kumar, Harsh Deep, Kamla Malik, Preety Verma, Pankaj Yadav, Sheetal Kumari, Aakash, Sambandh Dhal

{"title":"Stem Rot of Pearl Millet Prevalence, Symptomatology, Disease Cycle, Disease Rating Scale and Pathogen Characterization in Pearl Millet-Klebsiella Pathosystem.","authors":"Vinod Kumar Malik, Pooja Sangwan, Manjeet Singh, Pavitra Kumari, Niharika Shoeran, Navjeet Ahalawat, Mukesh Kumar, Harsh Deep, Kamla Malik, Preety Verma, Pankaj Yadav, Sheetal Kumari, Aakash, Sambandh Dhal","doi":"10.5423/PPJ.OA.09.2023.0126","DOIUrl":"10.5423/PPJ.OA.09.2023.0126","url":null,"abstract":"The oldest and most extensively cultivated form of millet, known as pearl millet (Pennisetum glaucum (L.) R. Br. Syn. Pennisetum americanum (L.) Leeke), is raised over 312.00 lakh hectares in Asian and African countries. India is regarded as the significant hotspot for pearl millet diversity. In the Indian state of Haryana, where pearl millet is grown, a new and catastrophic bacterial disease known as stem rot of pearl millet spurred by the bacterium Klebsiella aerogenes (formerly Enterobacter) was first observed during fall 2018. The disease appears in form of small to long streaks on leaves, lesions on stem, and slimy rot appearance of stem. The associated bacterium showed close resemblance to Klebsiella aerogenes that was confirmed by a molecular evaluation based on 16S rDNA and gyrA gene nucleotide sequences. The isolates were also identified to be Klebsiella aerogenes based on biochemical assays, where Klebsiella isolates differed in D-trehalose and succinate alkalisation tests. During fall 2021-2023, the disease has spread all the pearl millet-growing districts of the state, extending up to 70% disease incidence in the affected fields. The disease is causing considering grain as well as fodder losses. The proposed scale, consisting of six levels (0-5), is developed where scores 0, 1, 2, 3, 4, and 5 have been categorized as highly resistant, resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible disease reaction, respectively. The disease cycle, survival of pathogen, and possible losses have also been studied to understand other features of the disease.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"48-58"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato. stercoris芽孢杆菌分离株B.PNR1作为番茄枯萎病防治和生长促进剂的评价和基因组挖掘。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.01.2023.0018

Rattana Pengproh, Thanwanit Thanyasiriwat, Kusavadee Sangdee, Juthaporn Saengprajak, Praphat Kawicha, Aphidech Sangdee

{"title":"Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato.","authors":"Rattana Pengproh, Thanwanit Thanyasiriwat, Kusavadee Sangdee, Juthaporn Saengprajak, Praphat Kawicha, Aphidech Sangdee","doi":"10.5423/PPJ.OA.01.2023.0018","DOIUrl":"10.5423/PPJ.OA.01.2023.0018","url":null,"abstract":"Recently, strategies for controlling Fusarium oxysporum f. sp. lycopersici (Fol), the causal agent of Fusarium wilt of tomato, focus on using effective biocontrol agents. In this study, an analysis of the biocontrol and plant growth promoting (PGP) attributes of 11 isolates of loamy soil Bacillus spp. has been conducted. Among them, the isolates B.PNR1 and B.PNR2 inhibited the mycelial growth of Fol by inducing abnormal fungal cell wall structures and cell wall collapse. Moreover, broad-spectrum activity against four other plant pathogenic fungi, F. oxysporum f. sp. cubense race 1 (Foc), Sclerotium rolfsii, Colletotrichum musae, and C. gloeosporioides were noted for these isolates. These two Bacillus isolates produced indole acetic acid, phosphate solubilization enzymes, and amylolytic and cellulolytic enzymes. In the pot experiment, the culture filtrate from B.PNR1 showed greater inhibition of the fungal pathogens and significantly promoted the growth of tomato plants more than those of the other treatments. Isolate B.PNR1, the best biocontrol and PGP, was identified as Bacillus stercoris by its 16S rRNA gene sequence and whole genome sequencing analysis (WGS). The WGS, through genome mining, confirmed that the B.PNR1 genome contained genes/gene cluster of a nonribosomal peptide synthetase/polyketide synthase, such as fengycin, surfactin, bacillaene, subtilosin A, bacilysin, and bacillibactin, which are involved in antagonistic and PGP activities. Therefore, our finding demonstrates the effectiveness of B. stercoris strain B.PNR1 as an antagonist and for plant growth promotion, highlighting the use of this microorganism as a biocontrol agent against the Fusarium wilt pathogen and PGP abilities in tomatoes.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"430-448"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bc/0b/ppj-oa-01-2023-0018.PMC10580056.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Damage Triggers the Activation of Immune Response to Viral Pathogens via Salicylic Acid in Plants. DNA损伤通过水杨酸触发植物对病毒病原体的免疫反应。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.08.2023.0112

Hwi-Won Jeong, Tae Ho Ryu, Hyo-Jeong Lee, Kook-Hyung Kim, Rae-Dong Jeong

{"title":"DNA Damage Triggers the Activation of Immune Response to Viral Pathogens via Salicylic Acid in Plants.","authors":"Hwi-Won Jeong, Tae Ho Ryu, Hyo-Jeong Lee, Kook-Hyung Kim, Rae-Dong Jeong","doi":"10.5423/PPJ.OA.08.2023.0112","DOIUrl":"10.5423/PPJ.OA.08.2023.0112","url":null,"abstract":"Plants are challenged by various pathogens throughout their lives, such as bacteria, viruses, fungi, and insects; consequently, they have evolved several defense mechanisms. In addition, plants have developed localized and systematic immune responses due to biotic and abiotic stress exposure. Animals are known to activate DNA damage responses (DDRs) and DNA damage sensor immune signals in response to stress, and the process is well studied in animal systems. However, the links between stress perception and immune response through DDRs remain largely unknown in plants. To determine whether DDRs induce plant resistance to pathogens, Arabidopsis plants were treated with bleomycin, a DNA damage-inducing agent, and the replication levels of viral pathogens and growth of bacterial pathogens were determined. We observed that DDR-mediated resistance was specifically activated against viral pathogens, including turnip crinkle virus (TCV). DDR increased the expression level of pathogenesis-related (PR) genes and the total salicylic acid (SA) content and promoted mitogen-activated protein kinase signaling cascades, including the WRKY signaling pathway in Arabidopsis. Transcriptome analysis further revealed that defense- and SA-related genes were upregulated by DDR. The atm-2atr-2 double mutants were susceptible to TCV, indicating that the main DDR signaling pathway sensors play an important role in plant immune responses. In conclusion, DDRs activated basal immune responses to viral pathogens.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"449-465"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/db/ppj-oa-08-2023-0112.PMC10580055.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Marker Development for the Rapid Differentiation of Black Rot Causing Xanthomonas campestris pv. campestris Race 7. 黄单胞菌黑腐病快速分化分子标记的建立。第七场比赛。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.07.2023.0102

Yeo-Hyeon Kim, Sopheap Mao, Nihar Sahu, Uzzal Somaddar, Hoy-Taek Kim, Masao Watanabe, Jong-In Park

{"title":"Molecular Marker Development for the Rapid Differentiation of Black Rot Causing Xanthomonas campestris pv. campestris Race 7.","authors":"Yeo-Hyeon Kim, Sopheap Mao, Nihar Sahu, Uzzal Somaddar, Hoy-Taek Kim, Masao Watanabe, Jong-In Park","doi":"10.5423/PPJ.OA.07.2023.0102","DOIUrl":"10.5423/PPJ.OA.07.2023.0102","url":null,"abstract":"Xanthomonas campestris pv. campestris (Xcc) is a plant pathogen of Brassica crops that causes black rot disease throughout the world. At present, 11 physiological races of Xcc (races 1-11) have been reported. The conventional method of using differential cultivars for Xcc race detection is not accurate and it is laborious and time-consuming. Therefore, the development of specific molecular markers has been used as a substitute tool because it offers an accurate and reliable result, particularly a quick diagnosis of Xcc races. Previously, our laboratory has successfully developed race-specific molecular markers for Xcc races 1-6. In this study, specific molecular markers to identify Xcc race 7 have been developed. In the course of study, whole genome sequences of several Xcc races, X. campestris pv. incanae, X. campestris pv. raphani, and X. campestris pv. vesicatoria were aligned to identify variable regions like sequence-characterized amplified regions and insertions and deletions specific to race 7. Primer pairs were designed targeting these regions and validated against 22 samples. The polymerase chain reaction analysis revealed that three primer pairs specifically amplified the DNA fragment corresponding to race 7. The obtained finding clearly demonstrates the efficiency of the newly developed markers in accurately detecting Xcc race 7 among the other races. These results indicated that the newly developed marker can successfully and rapidly detect Xcc race 7 from other races. This study represents the first report on the successful development of specific molecular markers for Xcc race 7.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"494-503"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ea/86/ppj-oa-07-2023-0102.PMC10580059.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Sensitive Detection of the Causal Agents of Postharvest Kiwifruit Rot, Botryosphaeria dothidea and Diaporthe eres, Using a Recombinase Polymerase Amplification Assay. 用重组酶-聚合酶扩增法快速、灵敏地检测猕猴桃采后腐烂病、斑点葡萄球菌和双孢子虫的致病因素。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.NT.07.2023.0094

Gi-Gyeong Park, Wonyong Kim, Kwang-Yeol Yang

{"title":"Rapid and Sensitive Detection of the Causal Agents of Postharvest Kiwifruit Rot, Botryosphaeria dothidea and Diaporthe eres, Using a Recombinase Polymerase Amplification Assay.","authors":"Gi-Gyeong Park, Wonyong Kim, Kwang-Yeol Yang","doi":"10.5423/PPJ.NT.07.2023.0094","DOIUrl":"10.5423/PPJ.NT.07.2023.0094","url":null,"abstract":"The occurrence of postharvest kiwifruit rot has caused great economic losses in major kiwifruit-producing countries. Several pathogens are involved in kiwifruit rot, notably Botryosphaeria dothidea, and Diaporthe species. In this study, a recombinase polymerase amplification (RPA) assay was developed for the rapid and sensitive detection of the pathogens responsible for posing significant threats to the kiwifruit industries. The RPA primer pairs tested in this study were highly specific for detection of B. dothidea and D. eres. The detection limits of our RPA assays were approximately two picograms of fungal genomic DNA. The optimal conditions for the RPA assays were determined to be at a temperature of 39°C maintained for a minimum duration of 5 min. We were able to detect the pathogens from kiwifruit samples inoculated with a very small number of conidia. The RPA assays enabled specific, sensitive, and rapid detection of B. dothidea and D. eres, the primary pathogens responsible for kiwifruit rots in South Korea.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"522-527"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b1/ef/ppj-nt-07-2023-0094.PMC10580058.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Mutation of a Putative NDP-Sugar Epimerase Gene in Ralstonia pseudosolanacearum Attenuates Exopolysaccharide Production and Bacterial Virulence in Tomato Plant. 假青枯菌NDP糖表位酶基因突变降低番茄外多糖产量和细菌毒力。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.06.2023.0090

Hyoung Ju Lee, Sang-Moo Lee, Minseo Choi, Joo Hwan Kwon, Seon-Woo Lee

{"title":"A Mutation of a Putative NDP-Sugar Epimerase Gene in Ralstonia pseudosolanacearum Attenuates Exopolysaccharide Production and Bacterial Virulence in Tomato Plant.","authors":"Hyoung Ju Lee, Sang-Moo Lee, Minseo Choi, Joo Hwan Kwon, Seon-Woo Lee","doi":"10.5423/PPJ.OA.06.2023.0090","DOIUrl":"10.5423/PPJ.OA.06.2023.0090","url":null,"abstract":"Ralstonia solanacearum species complex (RSSC) is a soil borne plant pathogen causing bacterial wilt on various important crops, including Solanaceae plants. The bacterial pathogens within the RSSC produce exopolysaccharide (EPS), a highly complicated nitrogen-containing heteropolymeric polysaccharide, as a major virulence factor. However, the biosynthetic pathway of the EPS in the RSSC has not been fully characterized. To identify genes in EPS production beyond the EPS biosynthetic gene operon, we selected the EPS-defective mutants of R. pseudosolanacearum strain SL341 from Tn5-inserted mutant pool. Among several EPS-defective mutants, we identified a mutant, SL341P4, with a Tn5-insertion in a gene encoding a putative NDP-sugar epimerase, a putative membrane protein with sugar-modifying moiety, in a reverse orientation to EPS biosynthesis gene cluster. This protein showed similar to other NDP-sugar epimerases involved in EPS biosynthesis in many phytopathogens. Mutation of the NDP-sugar epimerase gene reduced EPS production and biofilm formation in R. pseudosolanacearum. Additionally, the SL341P4 mutant exhibited reduced disease severity and incidence of bacterial wilt in tomato plants compared to the wild-type SL341 without alteration of bacterial multiplication. These results indicate that the NDP-sugar epimerase gene is required for EPS production and bacterial virulence in R. pseudosolanacearum.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"417-429"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/61/87/ppj-oa-06-2023-0090.PMC10580051.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of Fire Blight Blossom Infection Control Using Maryblyt in Korean Apple Orchards. 利用Maryblyt改良韩国苹果园火斑病防治技术。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.08.2023.0110

Kyung-Bong Namkung, Sung Chul Yun

{"title":"Improvement of Fire Blight Blossom Infection Control Using Maryblyt in Korean Apple Orchards.","authors":"Kyung-Bong Namkung, Sung Chul Yun","doi":"10.5423/PPJ.OA.08.2023.0110","DOIUrl":"10.5423/PPJ.OA.08.2023.0110","url":null,"abstract":"After transitioning from periodic to model-based control policy for fire blight blossom infection, it is crucial to provide the timing of field application with easy and accurate information. To assess the risk of blossom infection, Maryblyt was employed in 31 sites across apple-producing regions nationwide, including areas prone to fire blight outbreaks, from 2021 to 2023. In 2021 and 2023, two and seven sites experienced Blossom Infection Risk-Infection warning occurrences among 31 sites, respectively. However, in 2022, most of the sites observed Blossom Infection Risk-Infection from April 25 to 28, highlighting the need for blossom infection control. For the comparison between the two model-based control approaches, we established treatment 1, which involved control measures according to the Blossom Infection Risk-Infection warning and treatment 2, aimed at maintaining the Epiphytic Infection Potential below 100. The analysis of control values between these treatments revealed that treatment 2 was more effective in reducing Blossom Infection Risk-Infection and the number of days with Epiphytic Infection Potential above 100, with respective averages of 95.6% and 93.0% over the three years. Since 2022, the implementation of the K-Maryblyt system and the deployment of Automated Weather Stations capable of measuring orchard weather conditions, with an average of 10 stations per major apple fire blight county nationwide, have taken place. These advancements will enable the provision of more accurate and timely information for farmers based on fire blight models in the future.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"504-512"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9c/47/ppj-oa-08-2023-0110.PMC10580054.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus. 重组酶聚合酶扩增结合侧流条带快速检测豇豆轻度斑点病毒的研究进展。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.02.2023.0033

Xinyan Wu, Shuting Chen, Zixin Zhang, Yihan Zhang, Pingmei Li, Xinyi Chen, Miaomiao Liu, Qian Lu, Zhongyi Li, Zhongyan Wei, Pei Xu

{"title":"Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus.","authors":"Xinyan Wu, Shuting Chen, Zixin Zhang, Yihan Zhang, Pingmei Li, Xinyi Chen, Miaomiao Liu, Qian Lu, Zhongyi Li, Zhongyan Wei, Pei Xu","doi":"10.5423/PPJ.OA.02.2023.0033","DOIUrl":"10.5423/PPJ.OA.02.2023.0033","url":null,"abstract":"Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks. With the advancement in isothermal recombinase polymerase amplification and lateral flow strips technologies, more rapid and sensitive methods have become available for detecting this pathogen. In this study, we have developed a reverse transcription recombinase polymerase amplification combined with lateral flow strips (RT-RPA-LFS) method for the detection of CPMMV, specifically targeting the CPMMV coat protein (CP) gene. The RT-RPA-LFS assay only requires 20 min at 40°C and demonstrates high specificity. Its detection limit was 10 copies/μl, which is approximately up to 100 times more sensitive than RT-PCR on agarose gel electrophoresis. The developed RT-RPA-LFS method offers a rapid, convenient, and sensitive approach for field detection of CPMMV, which contribute to controlling the spread of the virus.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"486-493"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a7/29/ppj-oa-02-2023-0033.PMC10580057.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiology and Gene Expression Analysis of Tomato (Solanum lycopersicum L.) Exposed to Combined-Virus and Drought Stresses. 番茄（Solanum lycopersicum L.）在病毒和干旱联合胁迫下的生理和基因表达分析。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.07.2023.0103

Samra Mirzayeva, Irada Huseynova, Canan Yüksel Özmen, Ali Ergül

{"title":"Physiology and Gene Expression Analysis of Tomato (Solanum lycopersicum L.) Exposed to Combined-Virus and Drought Stresses.","authors":"Samra Mirzayeva, Irada Huseynova, Canan Yüksel Özmen, Ali Ergül","doi":"10.5423/PPJ.OA.07.2023.0103","DOIUrl":"10.5423/PPJ.OA.07.2023.0103","url":null,"abstract":"Crop productivity can be obstructed by various biotic and abiotic stresses and thus these stresses are a threat to universal food security. The information on the use of viruses providing efficacy to plants facing growth challenges owing to stress is lacking. The role of induction of pathogen-related genes by microbes is also colossal in drought-endurance acquisition. Studies put forward the importance of viruses as sustainable means for defending plants against dual stress. A fundamental part of research focuses on a positive interplay between viruses and plants. Notably, the tomato yellow leaf curl virus (TYLCV) and tomato chlorosis virus (ToCV) possess the capacity to safeguard tomato host plants against severe drought conditions. This study aims to explore the combined effects of TYLCV, ToCV, and drought stress on two tomato cultivars, Money Maker (MK, UK) and Shalala (SH, Azerbaijan). The expression of pathogen-related four cellulose synthase gene families (CesA/Csl) which have been implicated in drought and virus resistance based on gene expression analysis, was assessed using the quantitative real-time polymerase chain reaction method. The molecular tests revealed significant upregulation of Ces-A2, Csl-D3,2, and Csl-D3,1 genes in TYLCV and ToCV-infected tomato plants. CesA/Csl genes, responsible for biosynthesis within the MK and SH tomato cultivars, play a role in defending against TYLCV and ToCV. Additionally, physiological parameters such as \"relative water content,\" \"specific leaf weight,\" \"leaf area,\" and \"dry biomass\" were measured in dual-stressed tomatoes. Using these features, it might be possible to cultivate TYLCV-resistant plants during seasons characterized by water scarcity.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"466-485"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/55/0b/ppj-oa-07-2023-0103.PMC10580053.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant Protective and Growth Promoting Effects of Seed Endophytes in Soybean Plants. 大豆种子内生植物对植物的保护和促生长作用。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2023-10-01 DOI: 10.5423/PPJ.OA.06.2023.0091

Jiwon Kim, Seong-Ho Ahn, Ji Sun Yang, Seonwoo Choi, Ho Won Jung, Junhyun Jeon

{"title":"Plant Protective and Growth Promoting Effects of Seed Endophytes in Soybean Plants.","authors":"Jiwon Kim, Seong-Ho Ahn, Ji Sun Yang, Seonwoo Choi, Ho Won Jung, Junhyun Jeon","doi":"10.5423/PPJ.OA.06.2023.0091","DOIUrl":"10.5423/PPJ.OA.06.2023.0091","url":null,"abstract":"Seed-borne diseases reduce not only the seed germination and seedling growth but also seed quality, resulting in the significant yield loss in crop production. Plant seed harbors diverse microbes termed endophytes other than pathogens inside it. However, their roles and application to agricultures were rarely understood and explored to date. Recently, we had isolated from soybean seeds culturable endophytes exhibiting in-vitro antagonistic activities against common bacterial and fungal seed-borne pathogens. In this study, we evaluated effects of seed treatment with endophytes on plant growth and protection against the common seed-borne pathogens: four fungal pathogens (Cercospora sojina, C. kikuchii, Septoria glycines, Diaporthe eres) and two bacterial pathogens (Xanthomonas axonopodis pv. glycines, Pseudomonas syringae pv. tabaci). Our experiments showed that treatment of soybean seeds with seed endophytes clearly offer protection against seed-borne pathogens. We also found that some of the endophytes promote plant growth in addition to the disease suppression. Taken together, our results demonstrate agricultural potential of seed endophytes in crop protection.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"39 5","pages":"513-521"},"PeriodicalIF":2.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f5/57/ppj-oa-06-2023-0091.PMC10580052.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41209093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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