Plant Pathology Journal最新文献_第4页

Light- and Relative Humidity-Regulated Hypersensitive Cell Death and Plant Immunity in Chinese Cabbage Leaves by a Non-adapted Bacteria Xanthomonas campestris pv. vesicatoria. 非适应细菌野油菜黄单胞菌 pv. vesicatoria 对光照和相对湿度调控的大白菜叶片超敏细胞死亡和植物免疫作用

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.03.2024.0057

Young Hee Lee, Yun-Hee Kim, Jeum Kyu Hong

{"title":"Light- and Relative Humidity-Regulated Hypersensitive Cell Death and Plant Immunity in Chinese Cabbage Leaves by a Non-adapted Bacteria Xanthomonas campestris pv. vesicatoria.","authors":"Young Hee Lee, Yun-Hee Kim, Jeum Kyu Hong","doi":"10.5423/PPJ.OA.03.2024.0057","DOIUrl":"10.5423/PPJ.OA.03.2024.0057","url":null,"abstract":"Inoculation of Chinese cabbage leaves with high titer (107 cfu/ml) of the non-adapted bacteria Xanthomonas campestris pv. vesicatoria (Xcv) strain Bv5-4a.1 triggered rapid leaf tissue collapses and hypersensitive cell death (HCD) at 24 h. Electrolyte leakage and lipid peroxidation markedly increased in the Xcv-inoculated leaves. Defence-related gene expressions (BrPR1, BrPR4, BrChi1, BrGST1 and BrAPX1) were preferentially activated in the Xcv-inoculated leaves. The Xcv-triggered HCD was attenuated by continuous light but accelerated by a dark environment, and the prolonged high relative humidity also alleviated the HCD. Constant dark and increased relative humidity provided favorable conditions for the Xcv bacterial growth in the leaves. Pretreated fluridone (biosynthetic inhibitor of endogenous abscisic acid [ABA]) increased the HCD in the Xcv-inoculated leaves, but exogenous ABA attenuated the HCD. The pretreated ABA also reduced the Xcv bacterial growth in the leaves. These results highlight that the onset of HCD in Chinese cabbage leaves initiated by non-adapted pathogen Xcv Bv5-4a.1 and in planta bacterial growth was differently modulated by internal and external conditional changes.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"358-376"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Application of Reverse Transcription Nanoplate-Based Digital PCR Assay for Sensitive and Accurate Detection of Rice Black-Streaked Dwarf Virus in Cereal Crops. 基于反转录纳米片的数字 PCR 检测方法的开发与应用，用于灵敏、准确地检测谷类作物中的水稻黑条矮缩病病毒。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.NT.03.2024.0048

Hyo-Jeong Lee, Hae-Jun Kim, Sang-Min Kim, Rae-Dong Jeong

{"title":"Development and Application of Reverse Transcription Nanoplate-Based Digital PCR Assay for Sensitive and Accurate Detection of Rice Black-Streaked Dwarf Virus in Cereal Crops.","authors":"Hyo-Jeong Lee, Hae-Jun Kim, Sang-Min Kim, Rae-Dong Jeong","doi":"10.5423/PPJ.NT.03.2024.0048","DOIUrl":"10.5423/PPJ.NT.03.2024.0048","url":null,"abstract":"The emergence of rice black-streaked dwarf virus (RBSDV) poses a significant threat to global cereal crop cultivation, necessitating the urgent development of reliable detection and quantification techniques. This study introduces a reliable approach for the precise and sensitive quantification of the RBSDV in cereal crop samples, employing a reverse transcription digital polymerase chain reaction (RT-dPCR) assay. We assessed the specificity and sensitivity of the RT-dPCR assay proposed for precise RBSDV detection and quantification. Our findings demonstrate that RT-dPCR was specific for detection of RBSDV, with no cross-reactivity observed with other viruses infecting cereal crops. The RT-dPCR sensitivity was over 10 times that of RT-quantitative PCR (RT-qPCR). The detection limit of RT-dPCR was 0.096 copies/μl. In addition, evaluation of RT-dPCR assay with field samples was conducted on 60 different cereal crop samples revealed that RT-dPCR (45/60) exhibited superior accuracy compared with RT-qPCR (23/60). In this study, we present a specific and accurate RT-dPCR assay for the detection and quantification of RBSDV.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"408-413"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia. 导致印度尼西亚芒果枯萎病的 Colletotrichum spp.

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.NT.04.2024.0061

Khaerani Nurlaelita, Arif Wibowo, Ani Widiastuti

{"title":"Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia.","authors":"Khaerani Nurlaelita, Arif Wibowo, Ani Widiastuti","doi":"10.5423/PPJ.NT.04.2024.0061","DOIUrl":"10.5423/PPJ.NT.04.2024.0061","url":null,"abstract":"Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch's Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"399-407"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum. 对禾谷镰刀菌中作为包囊形成诱导潜在信号的气生菌丝崩溃过程中特异表达的基因进行功能分析

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.FT.01.2024.0007

Yun-Seon Choi, Da-Woon Kim, Sung-Hwan Yun

{"title":"Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum.","authors":"Yun-Seon Choi, Da-Woon Kim, Sung-Hwan Yun","doi":"10.5423/PPJ.FT.01.2024.0007","DOIUrl":"10.5423/PPJ.FT.01.2024.0007","url":null,"abstract":"Fusarium graminearum, the causal agent of Fusarium head blight (FHB) in cereal crops, employs the production of sexual fruiting bodies (perithecia) on plant debris as a strategy for overwintering and dissemination. In an artificial condition (e.g., carrot agar medium), the F. graminearum Z3643 strain was capable of producing perithecia predominantly in the central region of the fungal culture where aerial hyphae naturally collapsed. To unravel the intricate relationship between natural aerial hyphae collapse and sexual development in this fungus, we focused on 699 genes differentially expressed during aerial hyphae collapse, with 26 selected for further analysis. Targeted gene deletion and quantitative real-time PCR analyses elucidated the functions of specific genes during natural aerial hyphae collapse and perithecium formation. Furthermore, comparative gene expression analyses between natural collapse and artificial removal conditions reveal distinct temporal profiles, with the latter inducing a more rapid and pronounced response, particularly in MAT gene expression. Notably, FGSG_09210 and FGSG_09896 play crucial roles in sexual development and aerial hyphae growth, respectively. Taken together, it is plausible that if aerial hyphae collapse occurs on plant debris, it may serve as a physical cue for inducing perithecium formation in crop fields, representing a survival strategy for F. graminearum during winter. Insights into the molecular mechanisms underlying aerial hyphae collapse provides offer potential strategies for disease control against FHB caused by F. graminearum.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"83-97"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Antagonistic Bacillus against Brown Rot in Dendrocalamus latiflorus and Preparation of Applying Bacterial Suspension. 筛选抗扁柏褐腐病的拮抗芽孢杆菌并制备应用细菌悬浮液

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.07.2023.0107

Fengying Luo, Hang Chen, Wenjian Wei, Han Liu, Youzhong Chen, Shujiang Li

{"title":"Screening of Antagonistic Bacillus against Brown Rot in Dendrocalamus latiflorus and Preparation of Applying Bacterial Suspension.","authors":"Fengying Luo, Hang Chen, Wenjian Wei, Han Liu, Youzhong Chen, Shujiang Li","doi":"10.5423/PPJ.OA.07.2023.0107","DOIUrl":"10.5423/PPJ.OA.07.2023.0107","url":null,"abstract":"The aim of this study was to isolate biocontrol bacteria that could antagonize brown rot of Dendrocalamus latiflorus, optimize the culture conditions, and develop an effective biocontrol preparation for brown rot of D. latiflorus. This study isolated a bacterium with an antagonistic effect on bamboo brown rot from healthy D. latiflorus rhizosphere soil. Morphology, molecular biology, and physiological biochemistry methods identified it as Bacillus siamensis. The following culturing media and conditions improved the inhibition effect of B. siamensis: the best culturing media were 2% sucrose, 1.5% yeast extract, and 0.7% potassium chloride; the optimal culturing time, temperature, pH, and inoculation amount were 48 h, 30℃, 6, and 20%. The optimum formula of the applying bacterial suspension was 14% sodium dodecyl benzene sulfonate emulsifier, 4% Na2HPO4·2H2O, 0.3% hydroxypropyl methylcellulose thickener, and 20% B. siamensis. The pot experiment results showed the control effect of applying bacterial suspension, diluted 1,000 times is still better than that of 24% fenbuconazole suspension. The applying bacterial suspension enables reliable control of brown rot in D. latiflorus.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"1-15"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing. 利用元转录组和小 RNA 测序首次在栀子中发现的豆类常见花叶病毒分离株的分子特征。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.11.2023.0163

Zhong-Tian Xu, Hai-Tao Weng, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li, Yi-Yuan Li

{"title":"Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing.","authors":"Zhong-Tian Xu, Hai-Tao Weng, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li, Yi-Yuan Li","doi":"10.5423/PPJ.OA.11.2023.0163","DOIUrl":"10.5423/PPJ.OA.11.2023.0163","url":null,"abstract":"Gardenia (Gardenia jasminoides) is a popular and economically vital plant known for its ornamental and medicinal properties. Despite its widespread cultivation, there has been no documentation of plant viruses on gardenia yet. In the present study, gardenia leaves exhibiting symptoms of plant viral diseases were sampled and sequenced by both metatranscriptome and small RNA sequencing. As a consequence, bean common mosaic virus (BCMV) was identified in gardenia for the first time and named BCMV-gardenia. The full genome sequence of BCMV-gardenia is 10,054 nucleotides (nt) in length (excluding the poly (A) at the 3' termini), encoding a large polyprotein of 3,222 amino acids. Sequence analysis showed that the N-termini of the polyprotein encoded by BCMV-gardenia is less conserved when compared to other BCMV isolates, whereas the C-termini is the most conserved. Maximum likelihood phylogenetic analysis showed that BCMV-gardenia was clustered closely with other BCMV isolates identified outside the leguminous plants. Our results indicated that the majority of BCMV-gardenia virus-derived small interfering RNAs (vsiRNAs) were 21 nt and 22 nt, with 21 nt being more abundant. The first nucleotide at the 5' termini of vsiRNAs derived from BCMV-gardenia preferred U and A. The ratio of vsiRNAs derived from sense (51.1%) and antisense (48.9%) strands is approaching, and the distribution of vsiRNAs along the viral genome is generally even, with some hot spots forming in local regions. Our findings could provide new insights into the diversity, evolution, and host expansion of BCMV and contribute to the prevention and treatment of this virus.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"73-82"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-identification of Colletotrichum gloeosporioides Species Complex Isolates in Korea and Their Host Plants. 重新鉴定韩国的 Colletotrichum gloeosporioides 种复合分离株及其寄主植物。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.09.2023.0133

Le Dinh Thao, Hyorim Choi, Yunhee Choi, Anbazhagan Mageswari, Daseul Lee, Dong-Hyun Kim, Hyeon-Dong Shin, Hyowon Choi, Ho-Jong Ju, Seung-Beom Hong

{"title":"Re-identification of Colletotrichum gloeosporioides Species Complex Isolates in Korea and Their Host Plants.","authors":"Le Dinh Thao, Hyorim Choi, Yunhee Choi, Anbazhagan Mageswari, Daseul Lee, Dong-Hyun Kim, Hyeon-Dong Shin, Hyowon Choi, Ho-Jong Ju, Seung-Beom Hong","doi":"10.5423/PPJ.OA.09.2023.0133","DOIUrl":"10.5423/PPJ.OA.09.2023.0133","url":null,"abstract":"The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"16-29"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Inexpensive System for Rapid and Accurate On-site Detection of Garlic-Infected Viruses by Agarose Gel Electrophoresis Followed by Array Assay. 通过琼脂糖凝胶电泳和阵列检测快速准确地现场检测大蒜感染病毒的廉价系统。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.FT.11.2023.0146

Kazuyoshi Furuta, Shusuke Kawakubo, Jun Sasaki, Chikara Masuta

{"title":"An Inexpensive System for Rapid and Accurate On-site Detection of Garlic-Infected Viruses by Agarose Gel Electrophoresis Followed by Array Assay.","authors":"Kazuyoshi Furuta, Shusuke Kawakubo, Jun Sasaki, Chikara Masuta","doi":"10.5423/PPJ.FT.11.2023.0146","DOIUrl":"10.5423/PPJ.FT.11.2023.0146","url":null,"abstract":"Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"40-47"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Resistance Induction in Mungbean against Alternaria alternata through RNA Interference. 通过 RNA 干扰评估绿豆对交替孢霉的抗性诱导。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.OA.03.2023.0048

Hira Abbas, Nazia Nahid, Muhammad Shah Nawaz Ul Rehman, Tayyaba Shaheen, Sadia Liaquat

{"title":"Assessment of Resistance Induction in Mungbean against Alternaria alternata through RNA Interference.","authors":"Hira Abbas, Nazia Nahid, Muhammad Shah Nawaz Ul Rehman, Tayyaba Shaheen, Sadia Liaquat","doi":"10.5423/PPJ.OA.03.2023.0048","DOIUrl":"10.5423/PPJ.OA.03.2023.0048","url":null,"abstract":"A comprehensive survey of mungbean-growing areas was conducted to observe leaf spot disease caused by Alternaria alternata. Alternaria leaf spot symptoms were observed on the leaves. Diversity of 50 genotypes of mungbean was assessed against A. alternata and data on pathological traits was subjected to cluster analysis. The results showed that genotypes of mungbean were grouped into four clusters based on resistance parameters under the influence of disease. The principal component biplot demonstrated that all the disease-related parameters (% disease incidence, % disease intensity, lesion area, and % of infection) were strongly correlated with each other. Alt a 1 gene that is precisely found in Alternaria species and is responsible for virulence and pathogenicity. Alt a 1 gene was amplified using gene specific primers. The isolated pathogen produced similar symptoms when inoculated on mungbean and tobacco. The sequence analysis of the internal transcribed spacer (ITS) region, a 600 bp fragment amplified using specific primers, ITS1 and ITS2 showed 100% identity with A. alternata. Potato virus X (PVX) -based silencing vector expressing Alt a 1 gene was constructed to control this pathogen through RNA interference in tobacco. Out of 50 inoculated plants, 9 showed delayed onset of disease. Furthermore, to confirm our findings at molecular level semi-quantitative reverse transcriptase polymerase chain reaction was used. Both phenotypic and molecular investigation indicated that RNAi induced through the VIGS vector was efficacious in resisting the pathogen in the model host, Tobacco (Nicotiana tabacum). To the best of our knowledge, this study has been reported for the first time.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"59-72"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Insights into Abies koreana Drought Tolerance Conferred by Aureobasidium pullulans AK10. Aureobasidium pullulans AK10 对韩国赤松耐旱性的转录组学启示。

IF 2.3 3区农林科学

Plant Pathology Journal Pub Date : 2024-02-01 DOI: 10.5423/PPJ.FT.11.2023.0161

Jungwook Park, Mohamed Mannaa, Gil Han, Hyejung Jung, Hyo Seong Jeon, Jin-Cheol Kim, Ae Ran Park, Young-Su Seo

{"title":"Transcriptomic Insights into Abies koreana Drought Tolerance Conferred by Aureobasidium pullulans AK10.","authors":"Jungwook Park, Mohamed Mannaa, Gil Han, Hyejung Jung, Hyo Seong Jeon, Jin-Cheol Kim, Ae Ran Park, Young-Su Seo","doi":"10.5423/PPJ.FT.11.2023.0161","DOIUrl":"10.5423/PPJ.FT.11.2023.0161","url":null,"abstract":"The conservation of the endangered Korean fir, Abies koreana, is of critical ecological importance. In our previous study, a yeast-like fungus identified as Aureobasidium pullulans AK10, was isolated and shown to enhance drought tolerance in A. koreana seedlings. In this study, the effectiveness of Au. pullulans AK10 treatment in enhancing drought tolerance in A. koreana was confirmed. Furthermore, using transcriptome analysis, we compared A. koreana seedlings treated with Au. pullulans AK10 to untreated controls under drought conditions to elucidate the molecular responses involved in increased drought tolerance. Our findings revealed a predominance of downregulated genes in the treated seedlings, suggesting a strategic reallocation of resources to enhance stress defense. Further exploration of enriched Kyoto Encyclopedia of Genes and Genomes pathways and protein-protein interaction networks revealed significant alterations in functional systems known to fortify drought tolerance, including the terpenoid backbone biosynthesis, calcium signaling pathway, pyruvate metabolism, brassinosteroid biosynthesis, and, crucially, flavonoid biosynthesis, renowned for enhancing plant drought resistance. These findings deepen our comprehension of how AK10 biostimulation enhances the resilience of A. koreana to drought stress, marking a substantial advancement in the effort to conserve this endangered tree species through environmentally sustainable treatment.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 1","pages":"30-39"},"PeriodicalIF":2.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0