Plant Pathology Journal最新文献_第3页

Molecular Identification and Genetic Diversity Analysis of Papaya Leaf Curl China Virus Infecting Ageratum conyzoides. 感染Ageratum conyzoides的中国木瓜卷叶病毒的分子鉴定和遗传多样性分析

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.NT.04.2024.0066

Liping Zhang, Shujie Wu, Meisheng Zhao, Hussein Ghanem, Gentu Wu, Mingjun Li, Ling Qing

{"title":"Molecular Identification and Genetic Diversity Analysis of Papaya Leaf Curl China Virus Infecting Ageratum conyzoides.","authors":"Liping Zhang, Shujie Wu, Meisheng Zhao, Hussein Ghanem, Gentu Wu, Mingjun Li, Ling Qing","doi":"10.5423/PPJ.NT.04.2024.0066","DOIUrl":"https://doi.org/10.5423/PPJ.NT.04.2024.0066","url":null,"abstract":"Papaya leaf curl China virus (PaLCuCNV) is a damaging plant pathogen causing substantial losses to crop. The complete genomes of three PaLCuCNV isolates from Ageratum conyzoides were obtained and combined with the 68 reference isolates in GenBank for comprehensive genetic diversity analyses using specialized computational tools. Sequence alignment revealed nucleotide sequence similarity ranging from 85.3% to 99.9% among 71 PaLCuCNV isolates. Employing phylogenetic analysis, 71 PaLCuCNV sequences were clustered into five groups, with no significant correlation observed between genetic differentiation and either host species or geographical origin. Additionally, 13 recombination events across all PaLCuCNV isolates were identified. Genetic diversity analysis indicated the ongoing expansion and evolution of PaLCuCNV populations, supported by a neutral model. Moreover, significant genetic differentiation was observed among distinct viral populations, primarily attributed to genetic drift. Overall, our findings provide valuable insights into the detection, genetic variation, and evolutionary dynamics of PaLCuCNV.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"551-558"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Resistance Acquisition and Mechanisms in Erwinia amylovora against Agrochemicals Used for Fire Blight Control. 比较 Erwinia amylovora 对用于控制火疫病的农用化学品的抗性获得和机制。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.07.2024.0106

Hyeonheui Ham, Ga-Ram Oh, Yong Hwan Lee, Yong Hoon Lee

{"title":"Comparison of Resistance Acquisition and Mechanisms in Erwinia amylovora against Agrochemicals Used for Fire Blight Control.","authors":"Hyeonheui Ham, Ga-Ram Oh, Yong Hwan Lee, Yong Hoon Lee","doi":"10.5423/PPJ.OA.07.2024.0106","DOIUrl":"https://doi.org/10.5423/PPJ.OA.07.2024.0106","url":null,"abstract":"Agrochemicals containing antibiotics are authorized to manage fire blight that has been occurring in Korea since 2015. The minimum inhibitory concentration (MIC) of each antibiotic against Erwinia amylovora, the causal pathogen of fire blight, has increased over the years due to the pathogen's frequent exposure to antibiotics, indicating the necessity to prepare for the emergence of antibiotic resistance. In this study, E. amylovora was exposed to stepwise increasing concentrations of eight different agrochemicals, each containing single or mixed antibiotics, and gene mutation and changes in MIC were assessed. Streptomycin and oxolinic acid induced an amino acid substitution in RpsL and GyrA, respectively, resulting in a rapid increase in MIC. Oxytetracycline initially induced amino acid substitutions or frameshifts in AcrR, followed by substitutions of 30S small ribosomal protein subunit S10 or AcrB, further increasing MIC. E. amylovora acquired resistance in the order of oxolinic acid, streptomycin, and oxytetracycline at varying exposure frequencies. Resistance acquisition was slower against agrochemicals containing mixed antibiotics than those with single antibiotics. However, gene mutations conferring antibiotic resistance emerged sequentially to both antibiotics in the mixed formulations. Results suggested that frequent application of mixed antibiotics could lead to the emergence of multidrug-resistant E. amylovora isolates. This study provided essential insights into preventing the emergence of antibiotic-resistant E. amylovora and understanding the underlying mechanisms of resistance acquisition.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"525-536"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of an SNP Marker for Identifying Xanthomonas oryzae pv. oryzae Thai Isolates That Break xa5-Mediated Bacterial Blight Resistance in Rice. 开发和验证 SNP 标记，用于识别破坏 xa5 介导的水稻细菌性枯萎病抗性的泰国黄单胞菌。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.04.2024.0070

Tebogo Balone, Ananda Nuryadi Pratama, Werapat Chansongkram, Thanita Boonsrangsom, Kawee Sujipuli, Kumrop Ratanasut

{"title":"Development and Validation of an SNP Marker for Identifying Xanthomonas oryzae pv. oryzae Thai Isolates That Break xa5-Mediated Bacterial Blight Resistance in Rice.","authors":"Tebogo Balone, Ananda Nuryadi Pratama, Werapat Chansongkram, Thanita Boonsrangsom, Kawee Sujipuli, Kumrop Ratanasut","doi":"10.5423/PPJ.OA.04.2024.0070","DOIUrl":"https://doi.org/10.5423/PPJ.OA.04.2024.0070","url":null,"abstract":"Xanthomonas oryzae pv. oryzae (Xoo) is a pathogenic bacterium responsible for bacterial blight (BB) disease in rice, primarily mediated by the interaction between the plant and pathogen. The virulence mechanism involves the activation of the Sugars Will Eventually be Exported Transporter (SWEET) gene family in rice by transcription activator-like effectors derived from Xoo. The BB resistance gene xa5 has been identified as one of the most effective genes against Thai Xoo isolates, but xa5-mediated resistance-breaking Xoo strains have emerged. This study aimed to develop a single nucleotide polymorphism (SNP) marker for precise identification of xa5-mediated resistance-breaking Xoo. Comparative genomics of Thai Xoo isolates Xoo16PK001 and Xoo16PK002, which were incompatible and compatible with rice variety IRBB5 carrying xa5, respectively, identified eight SNP positions for the development of an SNP marker. The SNP marker XooE6 yields a specific 1,143 bp PCR product unique to Xoo16PK002. Screening 61 Thai isolates using XooE6 identified two positives: Xoo20PL010 and Xoo20UT002. Inoculation tests on rice varieties IRBB5 and IRBB13 demonstrated compatibility with IRBB5 and incompatibility with IRBB13, which bears Xa5 and xa13. Xoo16PK001 (XooE6-negative) showed different virulence. Inoculation on IRBB21 harboring Xa5, Xa13, and Xa21 resulted in partial resistance to both XooE6-positive and -negative strains. XooE6-positive strains up-regulated SWEET11 and suppressed SWEET14 in IRBB5, while Xoo16PK001 slightly induced SWEET11 but activated SWEET14 in IRBB13. This highlights the potential of XooE6 to identify xa5-mediated resistance-breaking Xoo strains and elucidate their pathogenic mechanisms through the upregulation of SWEET11.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"451-462"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput Sequencing Identified Multiple Fig Viruses and Viroids Associated with Fig Mosaic Disease in Iraq. 高通量测序鉴定出与伊拉克无花果马赛克病有关的多种无花果病毒和病毒株。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.04.2024.0068

Nabeel Al-Kaeath, Shrooq Zagier, Osamah Alisawi, Fadhal Al Fadhal, Naima Mahfoudhi

{"title":"High-Throughput Sequencing Identified Multiple Fig Viruses and Viroids Associated with Fig Mosaic Disease in Iraq.","authors":"Nabeel Al-Kaeath, Shrooq Zagier, Osamah Alisawi, Fadhal Al Fadhal, Naima Mahfoudhi","doi":"10.5423/PPJ.OA.04.2024.0068","DOIUrl":"https://doi.org/10.5423/PPJ.OA.04.2024.0068","url":null,"abstract":"Mosaic is the most common viral disease affecting fig plants. Although the Fig mosaic virus is the leading cause of mosaic disease, other viruses are also involved. High-throughput sequencing was used to assess viral infections in fig plants with mosaic. The genomic DNA and total RNAseq of mosaic-symptomatic fig leaves were sequenced using the Illumina platform. The analysis revealed the presence of fig badnavirus 1 (FBV-1), grapevine badnavirus 1 (GBV-1), citrus exocortis viroid (CEVd), and apple dimple fruit viroid (ADFVd). The FBV-1 and GBV-1 sequences were 7,140 bp and 7,239 bp long, respectively. The two genomes encode one open reading frame containing five major protein domains. The viroids, CEVd and ADFVd, were 397 bp and 305 bp long. Phylogenetic analyses revealed a close relationship between FBV-1 and Iranian isolates of the same species, while GBV-1 was closely related to Russian grapevine badnavirus isolates (Tem64, Blu17, KDH48, and Pal9). CEVd was closely related to other Iraqi isolates, while ADFVd was strongly related to a Spanish isolate. A registered endogenous pararetrovirus, caulimovirus-Fca1, with a size of 7,556 bp, was found in the RNA transcripts with a low expression level. This integrant was also detected in the genomes of the two lines 'Horaishi' (a female line) and 'Caprifig 6085' (a male line). Phylogenetic analyses revealed that caulimovirus-Fca1 was distinct from two other clades of different endogenous virus genera.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"486-497"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Antimicrobial Compounds Produced by Endolichenic Fungi in Different Culture Media. 研究内生真菌在不同培养基中产生的抗菌化合物

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.NT.06.2024.0087

Jaycee Augusto G Paguirigan, Eunah Jeong, Kyo Bin Kang, Jae-Seoun Hur, Wonyong Kim

{"title":"Investigation of Antimicrobial Compounds Produced by Endolichenic Fungi in Different Culture Media.","authors":"Jaycee Augusto G Paguirigan, Eunah Jeong, Kyo Bin Kang, Jae-Seoun Hur, Wonyong Kim","doi":"10.5423/PPJ.NT.06.2024.0087","DOIUrl":"https://doi.org/10.5423/PPJ.NT.06.2024.0087","url":null,"abstract":"Continuous use of synthetic fungicides has led to explosive emergence of fungicide-resistant microbes. Therefore, there are urgent needs for environmentally friendly antimicrobial agents with novel modes of action. This study investigated endolichenic fungi (ELF) as a source of antimicrobial compounds against various plant pathogens. We utilized an One Strain MAny Compounds (OSMAC) approach to enhance the chemical diversity of fourteen ELF. This involved cultivation of ELF in four growth media and subsequently assessing antimicrobial activities of culture extracts. Nearly half of the culture extracts exhibited antimicrobial activity against a Gram-positive bacterium, but showed minimal activity against Gram-negative bacteria tested. Notably, culture extracts from two ELF, Chaetomium globosum and Nodulisporium sp., demonstrated significant inhibitory effects against plant pathogenic fungi. LC-MS/MS-based metabolome profiling confirmed the presence of known bioactive compounds like cyclic dipeptides and chaetoglobosins. These findings highlight the effectiveness of combining OSMAC and metabolomics for identifying antimicrobial agents for agricultural use.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"559-567"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing Cold Plasma's Impact on Banana Growth and Fusarium Wilt Control. 评估冷等离子体对香蕉生长和镰刀菌枯萎病控制的影响

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-10-01 DOI: 10.5423/PPJ.OA.05.2024.0083

Priya Rajakumar, Nadiya Akmal Baharum, Afiqah Insyirah Lutfi, Najiah Mohd Sadali, Muhamad Shakirin Mispan, Lim Lian Kuang, Yap Seong Ling, Norzulaani Khalid, Nur Ardiyana Rejab

{"title":"Assessing Cold Plasma's Impact on Banana Growth and Fusarium Wilt Control.","authors":"Priya Rajakumar, Nadiya Akmal Baharum, Afiqah Insyirah Lutfi, Najiah Mohd Sadali, Muhamad Shakirin Mispan, Lim Lian Kuang, Yap Seong Ling, Norzulaani Khalid, Nur Ardiyana Rejab","doi":"10.5423/PPJ.OA.05.2024.0083","DOIUrl":"https://doi.org/10.5423/PPJ.OA.05.2024.0083","url":null,"abstract":"Bananas (Musa spp.), which serve millions of people worldwide, face a serious threat from Fusarium wilt (FW) disease caused by Fusarium oxysporum f. sp. cubense (Foc). Developing disease-resistant varieties particularly through breeding is challenging due to banana's seedless nature (parthenocarpic). As an alternative, cold plasma (CP) technology, has the potential to be used for crop improvement. Our study demonstrates a favourable impact of CP on the growth performance of banana (Berangan cultivar, AAA) in terms of height, leaf number and stem diameter. CP-treated plants also displayed delayed disease progression as well as lower disease severity indicated by slightly lower value of leaf symptoms index and rhizome discoloration index compared to the control plants. Additionally, quantitative real-time polymerase chain reaction analysis revealed differential expression of several defence (PR1, WRKY22, PAL, and CEBiP) and growth (Cytochrome P450, NAC68, and CAT) related genes in CP-treated plants, particularly in conjunction with Foc infection. These findings shed light on the potential use of CP in managing FW in banana and offer insights into possible mechanism behind improved traits.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 5","pages":"463-474"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Tissue-Specific Distribution and Genetic Variation of Alfalfa Mosaic Virus and Chinese Artichoke Mosaic Virus in Chinese Artichoke (Stachys affinis miq.). 紫花苜蓿花叶病毒和中国朝鲜蓟花叶病毒在中国朝鲜蓟（Stachys affinis miq.）

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.06.2024.0094

Ji-Soo Park, Dong-Joo Min, Tae-Seon Park, You-Seop Shin, Jin-Sung Hong

{"title":"Investigation of Tissue-Specific Distribution and Genetic Variation of Alfalfa Mosaic Virus and Chinese Artichoke Mosaic Virus in Chinese Artichoke (Stachys affinis miq.).","authors":"Ji-Soo Park, Dong-Joo Min, Tae-Seon Park, You-Seop Shin, Jin-Sung Hong","doi":"10.5423/PPJ.OA.06.2024.0094","DOIUrl":"10.5423/PPJ.OA.06.2024.0094","url":null,"abstract":"The Chinese artichoke (Stachys affinis syn. S. sieboldii) is a widely cultivated crop, and its rhizome is used as a medicinal vegetable. To investigate the causes of viral diseases in Chinese artichokes, the infection rates of four virus species infecting Chinese artichoke were investigated. Since the Chinese artichoke propagates through its tuber, this study aimed to determine whether viral transmission to the progeny is possible through the tuber, by identifying the virus present in the tuber and investigating its accumulation. First, reverse transcription polymerase chain reaction analysis was performed to detect viruses using total RNA extracted from the flowers, leaves, and tubers of Chinese artichoke plants. Alfalfa mosaic virus (AMV) and Chinese artichoke mosaic virus (ChAMV) had high infectivity in Chinese artichoke and most plants were simultaneously infected with AMV and ChAMV. These viruses were present in all tissues, but their detection frequency and accumulation rates varied across different tissues of the Chinese artichoke. Also, we sequenced the coat protein (CP) genes of AMV and ChAMV to investigate genetic variations of virus between the leaf and tuber. It provides information on CP gene sequences and genetic diversity of isolates identified from new hosts of AMV and ChAMV. This study offers valuable insights into the distribution and spread of the ChAMV and AMV within Chinese artichoke plants, which have implications for the management and control of viral infections in crops.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"390-398"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Metatranscriptomic Analysis of Plant Viruses in Imported Frozen Cherries and Blueberries. 进口冷冻樱桃和蓝莓中植物病毒的元转录组学综合分析。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.06.2024.0088

Ga-Eun Lee, Hyo-Jeong Lee, Rae-Dong Jeong

{"title":"Comprehensive Metatranscriptomic Analysis of Plant Viruses in Imported Frozen Cherries and Blueberries.","authors":"Ga-Eun Lee, Hyo-Jeong Lee, Rae-Dong Jeong","doi":"10.5423/PPJ.OA.06.2024.0088","DOIUrl":"10.5423/PPJ.OA.06.2024.0088","url":null,"abstract":"The possibility of new viruses emerging in various regions worldwide has increased due to a combination of factors, including climate change and the expansion of international trading. Plant viruses spread through various transmission routes, encompassing well-known avenues such as pollen, seeds, and insects. However, research on potential transmission routes beyond these known mechanisms has remained limited. To address this gap, this study employed metatranscriptomic analysis to ascertain the presence of plant viruses in imported frozen fruits, specifically cherries and blueberries. This analysis aimed to identify pathways through which plant viruses may be introduced into countries. Virome analysis revealed the presence of six species of plant viruses in frozen cherries and blueberries: cherry virus A (CVA), prunus necrotic ringspot virus (PNRSV), prune dwarf virus (PDV), prunus virus F (PrVF), blueberry shock virus (BlShV), and blueberry latent virus (BlLV). Identifying these potential transmission routes is crucial for effectively managing and preventing the spread of plant viruses and crop protection. This study highlights the importance of robust quality control measures and monitoring systems for frozen fruits, emphasizing the need for proactive measures to mitigate the risk associated with the potential spread of plant viruses.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"377-389"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potentiality of Beneficial Microbe Bacillus siamensis GP-P8 for the Suppression of Anthracnose Pathogens and Pepper Plant Growth Promotion. 有益微生物暹罗芽孢杆菌 GP-P8 抑制炭疽病病原体和促进辣椒植株生长的潜力。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.01.2024.0022

Ji Min Woo, Hyun Seung Kim, In Kyu Lee, Eun Jeong Byeon, Won Jun Chang, Youn Su Lee

{"title":"Potentiality of Beneficial Microbe Bacillus siamensis GP-P8 for the Suppression of Anthracnose Pathogens and Pepper Plant Growth Promotion.","authors":"Ji Min Woo, Hyun Seung Kim, In Kyu Lee, Eun Jeong Byeon, Won Jun Chang, Youn Su Lee","doi":"10.5423/PPJ.OA.01.2024.0022","DOIUrl":"10.5423/PPJ.OA.01.2024.0022","url":null,"abstract":"This study was carried out to screen the antifungal activity against Colletotrichum acutatum, Colletotrichum dematium, and Colletotrichum coccodes. Bacterial isolate GP-P8 from pepper soil was found to be effective against the tested pathogens with an average inhibition rate of 70.7% in in vitro dual culture assays. 16S rRNA gene sequencing analysis result showed that the effective bacterial isolate as Bacillus siamensis. Biochemical characterization of GP-P8 was also performed. According to the results, protease and cellulose, siderophore production, phosphate solubilization, starch hydrolysis, and indole-3-acetic acid production were shown by the GP-P8. Using specific primers, genes involved in the production of antibiotics, such as iturin, fengycin, difficidin, bacilysin, bacillibactin, surfactin, macrolactin, and bacillaene were also detected in B. siamensis GP-P8. Identification and analysis of volatile organic compounds through solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) revealed that acetoin and 2,3-butanediol were produced by isolate GP-P8. In vivo tests showed that GP-P8 significantly reduced the anthracnose disease caused by C. acutatum, and enhanced the growth of pepper plant. Reverse transcription polymerase chain reaction analysis of pepper fruits revealed that GP-P8 treated pepper plants showed increased expression of immune genes such as CaPR1, CaPR4, CaNPR1, CaMAPK4, CaJA2, and CaERF53. These results strongly suggest that GP-P8 could be a promising biocontrol agent against pepper anthracnose disease and possibly a pepper plant growth-promoting agent.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"346-357"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RT-RPA Assay Combined with a Lateral Flow Strip to Detect Soybean Mosaic Virus. RT-RPA 分析法与侧流带相结合检测大豆花叶病毒。

IF 1.8 3区农林科学

Plant Pathology Journal Pub Date : 2024-08-01 DOI: 10.5423/PPJ.OA.02.2024.0027

Bong Geun Oh, Ju-Yeon Yoon, Ho-Jong Ju

{"title":"RT-RPA Assay Combined with a Lateral Flow Strip to Detect Soybean Mosaic Virus.","authors":"Bong Geun Oh, Ju-Yeon Yoon, Ho-Jong Ju","doi":"10.5423/PPJ.OA.02.2024.0027","DOIUrl":"10.5423/PPJ.OA.02.2024.0027","url":null,"abstract":"Soybean (Glycine max L.) is one of the most widely planted and used legumes in the world, being used for food, animal feed products, and industrial production. The soybean mosaic virus (SMV) is the most prevalent virus infecting soybean plants. This study developed a diagnostic method for the rapid and sensitive detection of SMV using a reverse transcription-recombinase polymerase amplification (RT-RPA) technique combined with a lateral flow strip (LFS). The RT-RPA and RT-RPA-LFS conditions to detect the SMV were optimized using the selected primer set that amplified part of the VPg protein gene. The optimized reaction temperature for the RT-RPA primer and RT-RPA-LFS primer used in this study was 38℃ for both, and the minimum reaction time was 10 min and 5 min, respectively. The RT-RPA-LFS was as sensitive as RT-PCR to detect SMV with 10 pg/μl of total RNA. The reliability of the developed RT-RPA-LFS assay was evaluated using leaves collected from soybean fields. The RT-RPA-LFS diagnostic method developed in this study will be useful as a diagnostic method that can quickly and precisely detect SMV in the epidemiological investigation of SMV, in the selection process of SMV-resistant varieties, on local farms with limited resources.","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"40 4","pages":"337-345"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0