Plant Pathology Journal最新文献

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Inhibition of Hop Stunt Viroid by Exogenous Double-Stranded RNA in Micropropagated Grapevine Plantlets. 外源双链RNA对酒花矮病毒类病毒在葡萄小苗中的抑制作用。
IF 2.5 3区 农林科学
Plant Pathology Journal Pub Date : 2025-08-01 DOI: 10.5423/PPJ.OA.05.2025.0071
Chae-Min Kang, Rae-Dong Jeong
{"title":"Inhibition of Hop Stunt Viroid by Exogenous Double-Stranded RNA in Micropropagated Grapevine Plantlets.","authors":"Chae-Min Kang, Rae-Dong Jeong","doi":"10.5423/PPJ.OA.05.2025.0071","DOIUrl":"10.5423/PPJ.OA.05.2025.0071","url":null,"abstract":"<p><p>Hop stunt viroid (HSVd) infection reduces grapevine (Vitis vinifera) yield and quality, leading to significant economic losses. Conventional methods for producing virus-free plantlets often fail to completely eliminate viroids, necessitating alternative strategies. In this study, RNA interference (RNAi) was induced by applying HSVd-specific double-stranded RNA (dsRNA) to infected grapevine plantlets. Exogenous dsRNA treatment significantly reduced HSVd levels, as confirmed by reverse transcription polymerase chain reaction and digital PCR. Fluorescently labeled (Cy3) dsRNA uptake was detected in plant tissues, while small RNA sequencing revealed an accumulation of HSVd-derived small interfering RNA, indicating RNAi activation. Notably, the inhibitory effect persisted through three successive generations without additional treatment, and similar suppression was observed in HSVd-infected cucumber plants. These findings highlight the efficacy and durability of exogenous dsRNA applications as a sustainable and non-transgenic approach for viroid control in grapevine cultivation.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 4","pages":"507-517"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12332490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effector-Triggered Immunity Is a Key Component of Nonhost Resistance in Nicotiana benthamiana against the Rice Blast Pathogen Magnaporthe oryzae. 效应触发免疫是本烟对稻瘟病菌非寄主抗性的关键组成部分。
IF 2.5 3区 农林科学
Plant Pathology Journal Pub Date : 2025-08-01 DOI: 10.5423/PPJ.OA.02.2025.0024
Jihyun Kim, Subin Lee, Min-Ki Seo, Dongmin Byun, Eunyoung Chae, Eunsook Park, Doil Choi
{"title":"Effector-Triggered Immunity Is a Key Component of Nonhost Resistance in Nicotiana benthamiana against the Rice Blast Pathogen Magnaporthe oryzae.","authors":"Jihyun Kim, Subin Lee, Min-Ki Seo, Dongmin Byun, Eunyoung Chae, Eunsook Park, Doil Choi","doi":"10.5423/PPJ.OA.02.2025.0024","DOIUrl":"10.5423/PPJ.OA.02.2025.0024","url":null,"abstract":"<p><p>Magnaporthe oryzae is the causal agent of rice blast disease, a major threat to global food security. Although M. oryzae infects a broad range of monocotyledonous plants, it fails to colonize dicot species such as Nicotiana benthamiana, offering a useful system to investigate nonhost resistance (NHR). In this study, we characterized the immune responses of N. benthamiana to M. oryzae by profiling defense-related gene expression, analyzing fungal invasion, and functionally dissecting key immune components. Time-course expression analyses revealed sustained upregulation of NbBAK1, NbEAS, NbWRKY22, and NbPR1, alongside dynamic regulation of NbCYP71D20 and NbSGT1. Virus-induced gene silencing demonstrated that silencing of NbSGT1, but not NbEAS or NbBAK1, significantly enhanced fungal colonization. Furthermore, salicylic acid (SA)-deficient NahG plants exhibited increased susceptibility, suggesting that SA and SGT1-dependent immunity synergistically contribute to NHR. Visualization of infection using a GFP-expressing fungal strain confirmed that suppression of SGT1 and SA signaling facilitated hyphal expansion into adjacent host cells. High-throughput screening of 179 M. oryzae candidate effectors revealed that 70 induced hypersensitive response-like cell death in N. benthamiana, a response that was abrogated by NbSGT1 silencing. These findings collectively demonstrate that SA signaling and SGT1-dependent effector-triggered immunity are critical barriers against M. oryzae invasion and highlight the potential of nonhost immune components as resources for engineering durable resistance in crops.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 4","pages":"472-483"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12332412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptome Analysis of Dryadomyces quercus-mongolicae, a Fungus Associated with Korean Oak Wilt Disease that Causes Oak Mortality in South Korea. 与导致韩国橡树死亡的韩国栎树枯萎病相关的真菌——蒙古栎树枯萎菌的转录组分析
IF 1.8 3区 农林科学
Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.NT.08.2024.0120
Hyowon Park, Jorge R Ibarra Caballero, Jane E Stewart, Ned B Klopfenstein, Uk Lee, Mee-Sook Kim
{"title":"Transcriptome Analysis of Dryadomyces quercus-mongolicae, a Fungus Associated with Korean Oak Wilt Disease that Causes Oak Mortality in South Korea.","authors":"Hyowon Park, Jorge R Ibarra Caballero, Jane E Stewart, Ned B Klopfenstein, Uk Lee, Mee-Sook Kim","doi":"10.5423/PPJ.NT.08.2024.0120","DOIUrl":"10.5423/PPJ.NT.08.2024.0120","url":null,"abstract":"<p><p>Korean oak wilt disease associated with Dryadomyces quercus-mongolicae recently emerged as a major tree disease in South Korea. A comprehensive transcriptome analysis is presented for D. quercus-mongolicae grown in vitro on three different culture media, identifying nearly 7,000 expressed transcripts. Most transcripts are associated with proteins essential for fungal survival and growth. The 40S ribosomal protein S25, ceramide very long chain fatty acid hydroxylase, Epl1 protein, and ADP/ATP translocase are particularly important due to their critical roles in the metabolism and environmental adaptation of fungi. Gene ontology analyses revealed that 39.4%, 61.2%, and 43.3% of transcripts were successfully annotated to biological process, molecular functions, and cellular component aspects, respectively. Furthermore, key metabolic pathways were elucidated, including sphingolipid metabolism, L-tryptophan biosynthesis, and glycolysis, which provide important information on physiological functioning of D. quercus-mongolicae. Overall, these findings provide key information on fundamental biological mechanisms of D. quercus-mongolicae.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"409-418"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deciphering Functions of a Putative Histidinol Dehydrogenase in Acidovorax citrulli by Phenotypic and Proteomic Analyses. 用表型和蛋白质组学分析解读瓜酸卵黄中一个假定的组氨酸二醇脱氢酶的功能。
IF 1.8 3区 农林科学
Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.03.2025.0036
Yongmin Cho, Haerim Rhyu, Suhyun Lee, Dohyun Kim, Dae Sung Kim, Jisun H J Lee, Sang-Wook Han
{"title":"Deciphering Functions of a Putative Histidinol Dehydrogenase in Acidovorax citrulli by Phenotypic and Proteomic Analyses.","authors":"Yongmin Cho, Haerim Rhyu, Suhyun Lee, Dohyun Kim, Dae Sung Kim, Jisun H J Lee, Sang-Wook Han","doi":"10.5423/PPJ.OA.03.2025.0036","DOIUrl":"10.5423/PPJ.OA.03.2025.0036","url":null,"abstract":"<p><p>Acidovorax citrulli (Ac) is a Gram-negative phytopathogenic bacterium causing bacterial fruit blotch (BFB) on cucurbit crops, specifically in the watermelon industry. However, cultivars of watermelon that are resistant to Ac have not been identified. Therefore, virulence factors/mechanisms in Ac must be characterized to develop alternative control strategies. Functions of a histidinol dehydrogenase, which is an essential enzyme for histidine biosynthesis, remain elusive in Ac. This study aims to elucidate the roles of histidinol dehydrogenase in Ac (HisDAc) using phenotype assays and proteomic analysis. The virulence of a mutant lacking a histidinol dehydrogenase, hisDAc:Tn5(EV), was diminished in geminated-seed inoculation and leaf infiltration assays, and the bacterium was impossible to grow without histidine in minimal media. However, treatment with exogenous histidine completely restored the virulence of the mutant on watermelon and its growth in minimal media, demonstrating that HisDAc is required for histidine biosynthesis, which contributes to virulence and growth. The comparative proteomic analysis indicates that HisDAc is involved in not only amino acid metabolism but also other biological mechanisms, including cell wall/membrane/envelope functions. This suggests that HisDAc may have pleiotropic effects. It was also confirmed that when Escherichia coli was incubated with Ac strains in water, the population level of E. coli increased in the presence of the mutant but not in the presence of the wild-type. This study leads to new insights regarding enzymes related to the production of primary metabolites and provides a promising target to discover an anti-virulence reagent to control BFB.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"341-351"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biocontrol Potential and Growth-Promoting Effects of Freshwater Trichoderma Strains against Plant Pathogenic Fungi in Red Pepper. 淡水木霉菌株对红辣椒植物病原真菌的生防潜力及促生作用
IF 1.8 3区 农林科学
Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.02.2025.0019
Yunjeong Heo, Gil Han, Hye Yeon Mun, Chang Soo Lee, Wonsu Cheon
{"title":"Biocontrol Potential and Growth-Promoting Effects of Freshwater Trichoderma Strains against Plant Pathogenic Fungi in Red Pepper.","authors":"Yunjeong Heo, Gil Han, Hye Yeon Mun, Chang Soo Lee, Wonsu Cheon","doi":"10.5423/PPJ.OA.02.2025.0019","DOIUrl":"10.5423/PPJ.OA.02.2025.0019","url":null,"abstract":"<p><p>The aim of this study was to investigate the biocontrol potential of Trichoderma spp. against plant pathogenic fungi. Forty-four Trichoderma strains isolated from freshwater environments were evaluated for their biocontrol potential against Phytophthora capsici and Fusarium solani, as well as for their siderophore production, phosphate solubilization, and enzymatic activities. Seven Trichoderma strains showed excellent performance and were selected for further experiments. These strains were identified as T. longibrachiatum and T. capillare based on the internal transcribed spacer and translational elongation factor 1-alpha gene sequences. The selected strains demonstrated strong antifungal activity against six fungal pathogens in dual-culture and volatile organic compound (VOC) assays. Strain FBCC-F1645 exhibited particularly high antifungal activity and completely inhibited the growth of P. capsici in the VOC assay. All the selected strains significantly enhanced the growth parameters of red pepper seedlings, and flowering was effectively promoted in the treatment groups. Additionally, these strains demonstrated preventive effects against Fusarium oxysporum and P. capsici, the causative agents of Fusarium wilt and Phytophthora blight, respectively, achieving notable control efficacy. Notably, strain FBCC-F1547 completely inhibited wilting and exhibited a strong preventive effect against blight. In the pepper anthracnose prevention experiment, all the tested strain suspensions (diluted 100-fold and 500-fold) effectively inhibited Colletotrichum acutatum. These findings suggest that Trichoderma spp. isolated from freshwater environments have the potential to reduce chemical pesticide use and promote sustainable agriculture.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"392-408"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predicting the Onset Date of Cucumber Powdery Mildew Based on Growing Degree Days and Leaf Wetness Duration in Greenhouse Environment. 温室环境下基于生长度数和叶片湿润期的黄瓜白粉病发病日期预测
IF 1.8 3区 农林科学
Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.FT.01.2025.0010
Min Son, Haejun Jeong, Jin-Yong Jung, Jiwon Park, Jiyoon Park, Hoyoung Park, Jonghan Yoon, Se-Hoon Jung, Chun-Bo Sim, Kwang-Hyung Kim, Sook-Young Park
{"title":"Predicting the Onset Date of Cucumber Powdery Mildew Based on Growing Degree Days and Leaf Wetness Duration in Greenhouse Environment.","authors":"Min Son, Haejun Jeong, Jin-Yong Jung, Jiwon Park, Jiyoon Park, Hoyoung Park, Jonghan Yoon, Se-Hoon Jung, Chun-Bo Sim, Kwang-Hyung Kim, Sook-Young Park","doi":"10.5423/PPJ.FT.01.2025.0010","DOIUrl":"10.5423/PPJ.FT.01.2025.0010","url":null,"abstract":"<p><p>Cucumber powdery mildew, caused by Podosphaera xanthii, can lead to significant yield losses in greenhouse cultivation. A calendar-based fungicide spray program is commonly employed by farmers, often leading to excessive spraying irrespective of disease conduciveness under certain weather conditions. Therefore, a disease model that can predict the onset of symptoms for determining when to start the first spray applications during a season is needed. This study developed a disease onset forecasting model, which uses growing degree days and leaf wetness duration as input variables, to aid the spray program for cucumber powdery mildew in the greenhouse environment. The model was calibrated using disease onset dates and corresponding weather data collected from two consecutive greenhouse experiments in 2022. As a result, we successfully simulated the symptom onset date with a margin of error of 5.5 days across two validation trials in 2023 and 2024. Further improvements to the model are needed to establish a model-based fungicide program in the greenhouse environment, which can be done by securing more data from additional trials for further modification and calibration of the model.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"419-424"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Re-assessment of Taxonomy and Host Range of Colletotrichum from Korea: Focus on the C. boninense, C. spaethianum Species Complexes, and Related Taxa. 韩国炭疽菌的分类及寄主范围的再评价——以炭疽菌属C. boninense、C. spaethium种复合体及相关分类群为中心。
IF 1.8 3区 农林科学
Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.01.2025.0002
Le Dinh Thao, Hyorim Choi, Donghun Kang, Anbazhagan Mageswari, Jae Sung Lee, Daseul Lee, In-Young Choi, Hyeon-Dong Shin, Seung-Beom Hong
{"title":"Re-assessment of Taxonomy and Host Range of Colletotrichum from Korea: Focus on the C. boninense, C. spaethianum Species Complexes, and Related Taxa.","authors":"Le Dinh Thao, Hyorim Choi, Donghun Kang, Anbazhagan Mageswari, Jae Sung Lee, Daseul Lee, In-Young Choi, Hyeon-Dong Shin, Seung-Beom Hong","doi":"10.5423/PPJ.OA.01.2025.0002","DOIUrl":"10.5423/PPJ.OA.01.2025.0002","url":null,"abstract":"<p><p>Colletotrichum species are commonly known as important phytopathogens causing anthracnose in Korea and worldwide, with a diverse range of host plants. Colletotrichum isolates preserved in the Korean Agricultural Culture Collection (KACC) are important resources for scientific research as well as anthracnose disease management strategies in Korea. Many Colletotrichum isolates in KACC had been identified using morphological characteristics and their host plants by depositors, this could lead to inaccurate species names. In this study, 38 KACC isolates were, therefore, re-identified as 13 known species (C. boninense, C. caudasporum, C. coccodes, C. echinochloae, C. karsti, C. liriopes, C. nigrum, C. sansevieriae, C. spaethianum, C. sublineola, C. sydowii, C. truncatum, and C. zhaoqingense) and a new species candidate, based on multi-locus sequence analyses of the nuclear ribosomal internal transcribed spacers, glyceraldehyde-3-phosphate dehydrogenase (gapdh), chitin synthase 1 (chs-1), histone-3 (his3), actin (act), beta-tubulin 2 (tub2), and manganese-superoxide dismutase (sod2). Of these, C. caudasporum, C. echinochloae, and C. zhaoqingense are unrecorded species in Korea. The results also revealed 16 new host-fungus combinations in Korea, including 13 new combinations worldwide. However, the pathogenicity of the fungal species in this work on their hosts was not confirmed.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"367-379"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Induced Resistance against Pseudomonas syringae pv. lachrymans in Cucumber by Spraying Cell-Free Microalgae Supernatant. 丁香假单胞菌诱导抗性研究。喷洒无细胞微藻上清液对黄瓜生长的影响。
IF 1.8 3区 农林科学
Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.FT.02.2025.0028
Sang-Moo Lee, Jin-Soo Son, Bongsoo Lee, Yong-Keun Chang, Chang-Ki Shim, Choong-Min Ryu
{"title":"Induced Resistance against Pseudomonas syringae pv. lachrymans in Cucumber by Spraying Cell-Free Microalgae Supernatant.","authors":"Sang-Moo Lee, Jin-Soo Son, Bongsoo Lee, Yong-Keun Chang, Chang-Ki Shim, Choong-Min Ryu","doi":"10.5423/PPJ.FT.02.2025.0028","DOIUrl":"10.5423/PPJ.FT.02.2025.0028","url":null,"abstract":"<p><p>Chlorella is a genus of aquatic photosynthetic microalgae used in the production of dietary supplements, cosmetics, and biofuels, and also recently utilized as biological control agents or biofertilizers in agricultural supplements. Chlorella supernatant elicits induced resistance in Arabidopsis thaliana but its effects on crop plants remain largely unknown. This study tested whether application of Chlorella supernatant elicited induced resistance in cucumber (Cucumis sativus L.). Foliar application of supernatants from six microalgal strains revealed that supernatants from the high biofuel-producing strains HS2 and ABC001 elicited induced resistance against Pseudomonas syringae pv. lachrymans, which causes angular leaf spot in cucumber. In addition, spraying plants with D-lactic acid, a previously known determinant of induced resistance in the Chlorella supernatant, reduced the severity of disease caused by P. syringae pv. lachrymans in cucumber leaves by activating the salicylic acid and jasmonic acid signaling pathways. The application of Chlorella supernatant thus protects a crop plant against disease while offering a cost-effective method of recycling waste supernatant.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"321-329"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Profiling and Precise Diagnosis of Pratylenchus penetrans Infestation in Soil: A qPCR-Based Molecular Approach. 基于qpcr的土壤渗透叶青虫侵染分子分析与精确诊断
IF 1.8 3区 农林科学
Plant Pathology Journal Pub Date : 2025-06-01 DOI: 10.5423/PPJ.OA.11.2024.0181
Karthi Natesan, Byeong-Yong Park, Hyoung-Rai Ko, Eunhwa Kim, Sohee Park, Sekeun Park
{"title":"Molecular Profiling and Precise Diagnosis of Pratylenchus penetrans Infestation in Soil: A qPCR-Based Molecular Approach.","authors":"Karthi Natesan, Byeong-Yong Park, Hyoung-Rai Ko, Eunhwa Kim, Sohee Park, Sekeun Park","doi":"10.5423/PPJ.OA.11.2024.0181","DOIUrl":"10.5423/PPJ.OA.11.2024.0181","url":null,"abstract":"<p><p>Pratylenchus penetrans, an important soil pathogen, has been reported on various crops in the temperate regions of South Korea. In concern, there is an urgent need for a precise, species-specific quantitative polymerase chain reaction (qPCR) kit to detect and quantify root lesion nematodes for early pest management and to controls yield losses. The present study focuses on D2-D3 region, a known marker for molecular profiling of Pratylenchus sp. A primer set mined from the highly conserved D2-D3 region of P. penetrans was used in a SYBR green based qPCR assay. Initial examination identified P. penetrans from infested soil samples using morphological and phylogenetic analyses. The DPp7F12R primer set demonstrated significant specificity in identifying P. penetrants by both conventional polymerase chain reaction (PCR) and qPCR assays. Linear regression of serially diluted DNA from nematode and nematode inoculated soil revealed a limit of quantification of 2 picograms (r² = 0.984), while also highlighting the impact of soil inhibitors. The qPCR using the DNA from varying densities of P. penetrans inoculated in soil demonstrated a robust correlation (r² = 0.98), indicating the limit of detection down to single nematode. Primer specificity evaluation with field soil sample precisely detected only P. penetrants. Species-specific DPp7F12R facilitate the direct detection of P. penetrants from soil DNA in very shorter time. Reliability of PCR was confirmed using BLAST algorithm, which identified partial sequence of PCR amplicon (300 bp) as P. penetrants. Finally, PCR assay using DPp7F12R is crucial for early detection of P. penetrans infestations, helping improve the plant health.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 3","pages":"330-340"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Tomato Yellow Leaf Curl Virus-Induced Gene Expression Pattern in Tomato and Tobacco Plants. 番茄和烟草植株中 TYLCV 诱导基因表达模式的比较。
IF 1.8 3区 农林科学
Plant Pathology Journal Pub Date : 2025-06-01 Epub Date: 2025-04-03 DOI: 10.5423/PPJ.OA.12.2024.0191
Chenwei Zhang, Xin Jia, Xing Han, Yuan Cheng, Xiaocong Jiao, Guiyan Fan, Tiancong Ren, Xiaoli Ren, Yueyue Cai, Xuemei Zhang, Lu Li, Hongguang Pang, Zhonglin Shang
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