Inhibition of Hop Stunt Viroid by Exogenous Double-Stranded RNA in Micropropagated Grapevine Plantlets.

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Chae-Min Kang, Rae-Dong Jeong
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引用次数: 0

Abstract

Hop stunt viroid (HSVd) infection reduces grapevine (Vitis vinifera) yield and quality, leading to significant economic losses. Conventional methods for producing virus-free plantlets often fail to completely eliminate viroids, necessitating alternative strategies. In this study, RNA interference (RNAi) was induced by applying HSVd-specific double-stranded RNA (dsRNA) to infected grapevine plantlets. Exogenous dsRNA treatment significantly reduced HSVd levels, as confirmed by reverse transcription polymerase chain reaction and digital PCR. Fluorescently labeled (Cy3) dsRNA uptake was detected in plant tissues, while small RNA sequencing revealed an accumulation of HSVd-derived small interfering RNA, indicating RNAi activation. Notably, the inhibitory effect persisted through three successive generations without additional treatment, and similar suppression was observed in HSVd-infected cucumber plants. These findings highlight the efficacy and durability of exogenous dsRNA applications as a sustainable and non-transgenic approach for viroid control in grapevine cultivation.

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Abstract Image

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外源双链RNA对酒花矮病毒类病毒在葡萄小苗中的抑制作用。
啤酒花类病毒(HSVd)感染会降低葡萄产量和质量,导致重大经济损失。生产无病毒苗的常规方法往往不能完全消除类病毒,因此需要其他策略。在本研究中,利用hsvd特异性双链RNA (dsRNA)诱导感染葡萄植株的RNA干扰(RNAi)。逆转录聚合酶链反应和数字PCR证实,外源性dsRNA处理显著降低了HSVd水平。在植物组织中检测到荧光标记(Cy3) dsRNA摄取,而小RNA测序显示hsvd衍生的小干扰RNA积累,表明RNAi激活。值得注意的是,在没有额外处理的情况下,抑制作用持续了三代,在感染hsv的黄瓜植株中也观察到类似的抑制作用。这些发现强调了外源dsRNA作为一种可持续和非转基因的葡萄种植类病毒控制方法的有效性和持久性。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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