效应触发免疫是本烟对稻瘟病菌非寄主抗性的关键组成部分。

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Jihyun Kim, Subin Lee, Min-Ki Seo, Dongmin Byun, Eunyoung Chae, Eunsook Park, Doil Choi
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引用次数: 0

摘要

稻瘟病是全球粮食安全的一大威胁,稻瘟病是稻瘟病的致病因子。虽然M. oryzae侵染广泛的单子叶植物,但它不能定殖于双子叶植物,如Nicotiana benthamiana,为研究非寄主抗性(NHR)提供了一个有用的系统。在这项研究中,我们通过分析防御相关基因表达,分析真菌入侵,以及功能解剖关键免疫成分来表征benthamiana对m.o ryzae的免疫反应。时间过程表达分析显示NbBAK1、NbEAS、NbWRKY22和NbPR1持续上调,同时NbCYP71D20和NbSGT1动态上调。病毒诱导的基因沉默表明,NbSGT1的沉默,而NbEAS或NbBAK1的沉默,显著增强了真菌的定植。此外,水杨酸(SA)缺乏的NahG植物表现出更高的敏感性,表明SA和sgt1依赖性免疫协同促进了NHR。利用表达gfp的真菌菌株对感染进行可视化观察证实,抑制SGT1和SA信号有助于菌丝向邻近宿主细胞扩展。对179种M. oryzae候选效应物的高通量筛选显示,70种诱导benthamiana的超敏反应样细胞死亡,这种反应被NbSGT1沉默所消除。这些发现共同表明,SA信号和sgt1依赖的效应物触发的免疫是抵抗m.o ryzae入侵的关键屏障,并突出了非宿主免疫成分作为工程作物持久抗性资源的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effector-Triggered Immunity Is a Key Component of Nonhost Resistance in Nicotiana benthamiana against the Rice Blast Pathogen Magnaporthe oryzae.

Magnaporthe oryzae is the causal agent of rice blast disease, a major threat to global food security. Although M. oryzae infects a broad range of monocotyledonous plants, it fails to colonize dicot species such as Nicotiana benthamiana, offering a useful system to investigate nonhost resistance (NHR). In this study, we characterized the immune responses of N. benthamiana to M. oryzae by profiling defense-related gene expression, analyzing fungal invasion, and functionally dissecting key immune components. Time-course expression analyses revealed sustained upregulation of NbBAK1, NbEAS, NbWRKY22, and NbPR1, alongside dynamic regulation of NbCYP71D20 and NbSGT1. Virus-induced gene silencing demonstrated that silencing of NbSGT1, but not NbEAS or NbBAK1, significantly enhanced fungal colonization. Furthermore, salicylic acid (SA)-deficient NahG plants exhibited increased susceptibility, suggesting that SA and SGT1-dependent immunity synergistically contribute to NHR. Visualization of infection using a GFP-expressing fungal strain confirmed that suppression of SGT1 and SA signaling facilitated hyphal expansion into adjacent host cells. High-throughput screening of 179 M. oryzae candidate effectors revealed that 70 induced hypersensitive response-like cell death in N. benthamiana, a response that was abrogated by NbSGT1 silencing. These findings collectively demonstrate that SA signaling and SGT1-dependent effector-triggered immunity are critical barriers against M. oryzae invasion and highlight the potential of nonhost immune components as resources for engineering durable resistance in crops.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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