Intraspecific Grafting of Tomatoes: Impact of Disease-Resistant Rootstocks on Fusarium Wilt Prevention, Plant Growth, and Fruit Quality under Naturally Infested Field Conditions.

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Praphat Kawicha, Prakob Saman, Phatcharin Suwannachairob, Pancheewan Ponpang-Nga, Juthaporn Saengprajak, Aphidech Sangdee, Thanwanit Thanyasiriwat
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Abstract

Fusarium oxysporum f. sp. lycopersici (Fol) is a soilborne pathogen that causes vascular wilt in tomatoes, severely affecting yield and quality. Grafting susceptible scions onto resistant rootstocks is a promising control strategy. This study evaluated four resistant tomato accessions (LE314, LE472, LE482, and LE501) for their ability to suppress Fol translocation and support scion performance. PCR analysis showed that all resistant accessions restricted Fol movement beyond the roots, with no detection in shoot tissues, indicating effective containment of the pathogen. Gene expression profiling revealed distinct temporal and accession-specific responses of LRR, WRKY41, and PR-1 genes. In field trials, heterografted tomatoes remained symptomless across planting years, while self-grafted plants exhibited severe wilt symptoms. All grafted combinations achieved 100% success without signs of incompatibility. Growth parameters (plant height, branch number, and canopy diameter), fruit size, and yield did not differ significantly between self- and heterografted plants. Importantly, fruit quality assessment indicated that specific traits, particularly total soluble solids and fruit firmness, were influenced by scion-rootstock interactions, while fruit pH and color attributes (L*, a*, b*) remained stable across grafted treatments. These results confirm that resistant rootstocks can prevent Fol infection and maintain agronomic performance, supporting intraspecific grafting as an effective and sustainable approach for managing Fusarium wilt in tomato production.

番茄种内嫁接:在自然侵染条件下抗病砧木对枯萎病防治、植株生长和果实品质的影响
番茄枯萎病(Fusarium oxysporum f. sp. lycopersici, Fol)是一种引起番茄血管性枯萎病的土传病原菌,严重影响番茄的产量和品质。将易感接穗嫁接到抗性砧木上是一种很有前途的防治策略。本研究评估了4个抗病番茄品种(LE314、LE472、LE482和LE501)抑制Fol转运和支持接穗性能的能力。PCR分析结果显示,所有抗性材料都限制了叶片在根系以外的运动,而在茎部组织中没有检测到,表明病原菌得到了有效的遏制。基因表达谱揭示了LRR、WRKY41和PR-1基因不同的时间和准入特异性反应。在田间试验中,异种嫁接的番茄在整个种植年份都没有症状,而自嫁接的番茄则表现出严重的枯萎症状。所有的嫁接组合都取得了100%的成功,没有不相容性的迹象。自嫁接和异嫁接植株的生长参数(株高、分枝数和冠层直径)、果实大小和产量无显著差异。重要的是,果实质量评价表明,接穗-砧木相互作用影响了果实的某些性状,特别是总可溶性固结物和果实硬度,而果实pH和颜色属性(L*, a*, b*)在嫁接处理中保持稳定。这些结果证实,抗性砧木可以预防枯萎病感染并保持农艺性能,支持种内嫁接作为一种有效和可持续的番茄生产管理枯萎病的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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