Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Mud Flats in South Korea: Potential for Lettuce Growth Promotion and Control of Sclerotinia Rot.

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Jae-Uk Jee, Seog-Won Chang, Juhyun Ryu, Se-Chul Chun
{"title":"Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Mud Flats in South Korea: Potential for Lettuce Growth Promotion and Control of Sclerotinia Rot.","authors":"Jae-Uk Jee, Seog-Won Chang, Juhyun Ryu, Se-Chul Chun","doi":"10.5423/PPJ.OA.03.2025.0035","DOIUrl":null,"url":null,"abstract":"<p><p>Various strategies have been developed to control lettuce diseases on farms and in food-packing plants. Biological control is considered a promising alternative owing to its eco-friendly nature. In the present study, bacteria isolated from coastal mudflats were evaluated for their efficacy in controlling Sclerotinia rot, and the plant growth-promoting activity in lettuce was also assessed. Among the screened microorganisms from the coastal mudflats, 12 bacterial strains exhibited antifungal activity against Sclerotinia sclerotiorum selected. These isolates have shown beneficial characteristics, such as nitrogen fixation, indole-3-acetic acid production, phosphate solubilization, and siderophore production. Additionally, the selected isolates showed antifungal effects on the pathogens of major plant disease, such as Alternaria porri, Colletotrichum acutatum, Fusarium oxysporum, Phytophthora capsici, Pythium ultimum, Rhizoctonia solani, and Stemphylium lycopersici. Among the selected bacterial strains, Bacillus subtilis GCM190 exhibited a high sclerotinia rot control rate, similar to that of the tebuconazole-treated group, and showed a significant effect in promoting the growth of lettuce leaves, stems, and roots (least significant difference, P = 0.05). The selection of rifampicin-resistant mutants and their tracing on lettuce roots and soil confirmed that they were well established in both the soil and lettuce roots. The selected microorganisms also exhibited antifungal effects in vitro against other crop diseases affecting cucumbers, tomatoes, red peppers, and green onions, suggesting high potential for practical applications.</p>","PeriodicalId":20173,"journal":{"name":"Plant Pathology Journal","volume":"41 4","pages":"456-471"},"PeriodicalIF":2.5000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12332413/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Pathology Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5423/PPJ.OA.03.2025.0035","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Various strategies have been developed to control lettuce diseases on farms and in food-packing plants. Biological control is considered a promising alternative owing to its eco-friendly nature. In the present study, bacteria isolated from coastal mudflats were evaluated for their efficacy in controlling Sclerotinia rot, and the plant growth-promoting activity in lettuce was also assessed. Among the screened microorganisms from the coastal mudflats, 12 bacterial strains exhibited antifungal activity against Sclerotinia sclerotiorum selected. These isolates have shown beneficial characteristics, such as nitrogen fixation, indole-3-acetic acid production, phosphate solubilization, and siderophore production. Additionally, the selected isolates showed antifungal effects on the pathogens of major plant disease, such as Alternaria porri, Colletotrichum acutatum, Fusarium oxysporum, Phytophthora capsici, Pythium ultimum, Rhizoctonia solani, and Stemphylium lycopersici. Among the selected bacterial strains, Bacillus subtilis GCM190 exhibited a high sclerotinia rot control rate, similar to that of the tebuconazole-treated group, and showed a significant effect in promoting the growth of lettuce leaves, stems, and roots (least significant difference, P = 0.05). The selection of rifampicin-resistant mutants and their tracing on lettuce roots and soil confirmed that they were well established in both the soil and lettuce roots. The selected microorganisms also exhibited antifungal effects in vitro against other crop diseases affecting cucumbers, tomatoes, red peppers, and green onions, suggesting high potential for practical applications.

Abstract Image

Abstract Image

Abstract Image

韩国泥滩植物促生根杆菌的分离与鉴定:促进生菜生长和防治菌核腐病的潜力。
已经制定了各种策略来控制农场和食品包装工厂的莴苣疾病。生物防治因其生态友好性被认为是一种很有前途的替代方法。本研究从沿海滩涂中分离出细菌,对其防治菌核菌腐病的效果进行了评价,并对生菜的促生活性进行了评价。在筛选的沿海滩涂微生物中,筛选出12株对菌核菌具有抗真菌活性的菌株。这些菌株显示出有益的特性,如固氮、吲哚-3-乙酸生产、磷酸盐增溶和铁载体生产。此外,所选菌株对主要植物病害如黑孢霉、尖锐炭疽菌、尖孢镰刀菌、辣椒疫霉、最后圆霉、番茄枯核菌和番茄干霉均有一定的抑菌作用。在所选菌株中,枯草芽孢杆菌GCM190对生菜菌核腐病的控制率较高,与替布康唑处理组相似,对生菜叶、茎、根的生长均有显著促进作用(差异不显著,P = 0.05)。对利福平抗性突变体的筛选及其在生菜根系和土壤上的示踪证实了它们在土壤和生菜根系上的良好建立。所选微生物在体外对黄瓜、西红柿、红辣椒和大葱等其他作物病害也表现出抗真菌作用,表明具有很高的实际应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信