anandii链霉菌聚酮合成酶簇的功能表征

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Da-Ran Kim, Youn-Sig Kwak
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引用次数: 0

摘要

链霉菌以其抗真菌特性和产生多种次生代谢物而闻名,包括非核糖体肽和聚酮。这些代谢物可以通过各种遗传技术鉴定,允许使用全基因组数据库研究基因功能。许多研究利用CRISPR-Cas9诱变等先进技术来探索链霉菌的遗传功能,以产生位点特异性突变菌株。本研究通过全基因组测序和平均核苷酸同源性(ANI)分析,将Streptomyces sp. J6重新鉴定为anandii Streptomyces J6。利用CRISPR-Cas9进一步研究了II型和III型聚酮合成酶簇(PKS:簇9、10和12)的功能分析,揭示了srsA在烷基间苯二酚生物合成中的作用,烷基间苯二酚是一种具有抗真菌特性的酚类脂质。这些结果表明,链霉菌产生的多酮类代谢产物在微生物对植物病害的生物防治活性中起重要作用。此外,研究结果表明,特定的PKS分析可以快速有效地筛选大量候选微生物,从而促进有前途的生物防治剂的选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Functional Characterization of Polyketide Synthase Clusters in Streptomyces anandii J6.

Functional Characterization of Polyketide Synthase Clusters in Streptomyces anandii J6.

Functional Characterization of Polyketide Synthase Clusters in Streptomyces anandii J6.

Functional Characterization of Polyketide Synthase Clusters in Streptomyces anandii J6.

Streptomyces species are well-known for their antifungal properties and the production of diverse secondary metabolites, including non-ribosomal peptides and polyketides. These metabolites can be identified through various genetic techniques, allowing for the investigation of gene functions using whole-genome databases. Numerous studies have explored the genetic functions of Streptomyces using advanced techniques, such as CRISPR-Cas9 mutagenesis, to generate site-specific mutant strains. In this study, we re-identified Streptomyces sp. J6 as Streptomyces anandii J6 through whole-genome sequencing and average nucleotide identity (ANI) analysis. The type II and type III polyketide synthase clusters (PKS: clusters 9, 10, and 12) were further studied using CRISPR-Cas9 for functional analysis, revealing the role of srsA in the biosynthesis of alkylresorcinols, which are phenolic lipids with antifungal properties. These results indicate that metabolites belonging to the polyketide family produced by Streptomyces plays a significant role in the biocontrol activity of microorganisms against plant diseases. Furthermore, the findings suggest that specific PKS profiling enables the rapid and efficient screening of a large number of microbial candidates, thereby facilitating the selection of promising biocontrol agents.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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