韩国假单胞菌KF32和番茄假单胞菌KF45挥发性有机化合物对番茄青枯病的抑制作用

IF 2.5 3区 农林科学 Q2 PLANT SCIENCES
Mi Jin Jeon, Mee Kyung Sang
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引用次数: 0

摘要

青枯病(Ralstonia pseudosolanacearum)是一种具有广泛寄主范围和全球性影响的破坏性病害。为探索番茄青枯病的生态友好型生物防治策略,筛选了对番茄青枯病具有抑菌活性的挥发性有机化合物(VOCs)菌株。采用i型平板法测定了菌株产生的挥发性有机化合物的抑菌活性,并对番茄青枯病植株进行了测定。菌株KF32和KF45分别鉴定为韩国假单胞菌和fitomaticsa假单胞菌。它们的挥发性有机化合物能显著抑制假茄青霉的体外生长,降低番茄植株的发病率。对暴露于菌株KF32和KF45 VOCs的假茄青霉进行转录组学分析。RNA测序显示,来自KF32和KF45的VOCs下调了与细胞运动和病原体外源降解相关的基因。采用气相色谱-质谱法对菌株KF32和KF45产生的挥发性有机化合物进行了分析。鉴定的挥发性有机化合物中,菌株KF32产生的2-癸酮能显著抑制假茄青霉的生长,减轻番茄青枯病症状。本研究强调了产生挥发性有机化合物的假单胞菌菌株KF32和KF45作为生物防治剂的潜力,并有助于制定控制番茄细菌性枯萎病的长期策略。此外,了解挥发性有机化合物介导的相互作用为开发管理植物致病菌的改进策略提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Volatile Organic Compounds from Pseudomonas koreensis KF32 and P. fitomaticsae KF45 Suppress Ralstonia pseudosolanacearum and Reduce Bacterial Wilt in Tomato.

Bacterial wilt caused by Ralstonia pseudosolanacearum is a destructive disease with a broad host range and global impact. To explore eco-friendly biocontrol strategies for bacterial wilt, we screened Pseudomonas strains that produce volatile organic compounds (VOCs) with antibacterial activity against R. pseudosolanacearum and potential biocontrol effects on tomato bacterial wilt. We evaluated antibacterial activity of VOCs produced by bacterial strains using on I-plate, and conducted plant assays against of bacterial wilt tomato plant. Two strains, KF32 and KF45, were identified as Pseudomonas koreensis, and P. fitomaticsae, respectively. Their VOCs significantly inhibited R. pseudosolanacearum growth in vitro and reduced disease incidence in tomato plants. Transcriptomic analysis was performed on R. pseudosolanacearum exposed to VOCs from strains KF32 and KF45. RNA sequencing revealed that VOCs from KF32 and KF45 downregulated genes related to cell motility and xenobiotic degradation of the pathogen. We analyzed VOCs produced by strains KF32 and KF45 using gas chromatography-mass spectrometry. Among the identified VOCs, 2-decanone which was produced by strain KF32 significantly inhibited the growth of R. pseudosolanacearum and reduced tomato bacterial wilt symptoms. This study highlights the potential of VOC-producing Pseudomonas strains KF32 and KF45 as biocontrol agents and contributing to the development of long-term strategies for managing bacterial wilt in tomato. Furthermore, understanding VOC-mediated interactions provides valuable insights for developing improved strategies to manage plant pathogenic bacteria.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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