Phytochemistry最新文献

{"title":"Sesquiterpenoids from Achillea setacea Waldst. & Kit. with anti-inflammatory activity by inhibiting the glycolytic pathway","authors":"Gui-Min Xue ,&nbsp;Jin-Feng Xue ,&nbsp;Jiang-Jing Duan ,&nbsp;Hui-Ying Yang ,&nbsp;Yan-Jun Sun ,&nbsp;Hui Chen ,&nbsp;Yan-Le Zhi ,&nbsp;Zhi-Qiang Zhang ,&nbsp;Rao Li ,&nbsp;Dong-Rong Zhu ,&nbsp;Guo-Sheng Li","doi":"10.1016/j.phytochem.2025.114608","DOIUrl":"10.1016/j.phytochem.2025.114608","url":null,"abstract":"<div><div>Six previously undescribed sesquiterpenoids, named achisetaces A–F (<strong>1</strong>–<strong>6</strong>), involving a rare C₆–<em>O</em>–C₁₀ bridged guaianolide-type sesquiterpenoid (<strong>1</strong>), along with nine known analogues (<strong>7</strong>–<strong>15</strong>), were obtained from the aerial parts of <em>Achillea setacea</em> Waldst. &amp; Kit. The undescribed structures of <strong>1</strong>–<strong>6</strong> including their absolute configurations were determined through spectroscopic techniques (IR, UV, HRESIMS, 1D and 2D NMR), combined with DP4+ NMR analysis and quantum electronic circular dichroism (ECD) calculations. The inhibitory effects of compounds <strong>1</strong>–<strong>15</strong> on nitric oxide (NO) production were evaluated in LPS-induced RAW 264.7 cells. Compounds <strong>6</strong> and <strong>10</strong> exhibited significant anti-inflammatory activities with IC₅₀ values of 1.56 ± 0.32 and 6.89 ± 0.47 μM, respectively. Moreover, a mechanistic study revealed that compound <strong>6</strong> exerted the anti-inflammatory activity by regulating the glycolytic pathway <em>via</em> suppression of PFKFB3 activation.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114608"},"PeriodicalIF":3.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iridoids and other constituents from the aerial parts of Patrinia rupestris and their inhibitory effects on nitric oxide production","authors":"Zhi-Yuan Liu ,&nbsp;Sheng-Run Guo ,&nbsp;Jian-Xian Tang,&nbsp;Chun-Yan Zhao,&nbsp;Li Pu,&nbsp;Li-Zhong Wang,&nbsp;Dan Liu,&nbsp;Rong-Tao Li,&nbsp;Hong-Mei Li","doi":"10.1016/j.phytochem.2025.114609","DOIUrl":"10.1016/j.phytochem.2025.114609","url":null,"abstract":"<div><div>Forty-three compounds, including nine undescribed compounds, five iridoids (patrirupesins A−E, <strong>1</strong>−<strong>5</strong>) and four bis-iridoids (patrirupesins F–I, <strong>6</strong>−<strong>9</strong>), were isolated from the EtOAc-soluble fraction of the aerial parts of <em>Patrinia rupestris</em> (Pall.) Dufr., and their structures were elucidated based on Mass spectrometry and NMR spectroscopy. The absolute configuration of patrirupesins A−E (<strong>1</strong>−<strong>5</strong>) and I (<strong>9</strong>) was determined by experimental and calculated electronic circular dichroism (ECD). In addition, patrirupesin E (<strong>5</strong>), patrirupesin H (<strong>8</strong>), 8,9-didehydro-7-hydroxydolichodial (<strong>17</strong>), 5,6-epoxy-3-hydroxy-7-megastigmen-9-one (<strong>19</strong>), <em>iso</em>-seco-tanapartholide (<strong>24</strong>) and 3-hydroxy-2-methyl-4-(3-oxoprop-1-en-2-yl)cyclopent-1-enecarbaldehyde (<strong>43</strong>) showed <em>in vitro</em> anti-inflammatory activity on NO production in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) with IC₅₀ values of 9.32 ± 3.83, 4.63 ± 1.23, 8.31 ± 4.05, 17.23 ± 5.19, 8.30 ± 6.17 and 27.38 ± 4.69 μM, respectively.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114609"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome-level genome of Nardostachys jatamansi and comparative genomics analysis reveals its sesquiterpenoid biosynthesis","authors":"Xuexue Li ,&nbsp;Yuan Liu ,&nbsp;Xuan Liu ,&nbsp;Huachun Sheng ,&nbsp;Juan Li ,&nbsp;Wenbing Li ,&nbsp;Ying Li ,&nbsp;Chen Chen ,&nbsp;Jingqiu Feng ,&nbsp;Xinjia Yan ,&nbsp;Zhengming Yang ,&nbsp;Yanfei Huang ,&nbsp;Zhifeng Zhang ,&nbsp;Shaoshan Zhang","doi":"10.1016/j.phytochem.2025.114607","DOIUrl":"10.1016/j.phytochem.2025.114607","url":null,"abstract":"<div><div><em>Nardostachys jatamansi</em> (D. Don) DC., an aromatic and medicinal herb belonging to the <em>Nardostachys</em> genus within the Caprifoliaceae family, is globally well-known for its attractive perfume and health-promoting properties. However, genomic information specific to <em>N. jatamansi</em> remains largely unexplored. In this study, the first <em>de novo</em> assembly of the <em>N. jatamansi</em> genome was presented which spans approximately 2.88 Gb with the contig N50 of 9.92 Mb and scaffold N50 of 40.09 Mb. Using Hi-C sequencing data, we successfully anchored 98.53 % of the assembled sequences into 78 pseudochromosomes (6n = 13), a result corroborated by chromosome (cytogenetic) analysis. The genome comprises 92.33 % repeat sequences, including 73.82 % TEs and 18.51 % tandem repeats, as well as a total of 174,054 protein-coding genes, of which 93.62 % have been functionally annotated in databases such as NR, GO, KEGG, Interpro, and others. In addition, our analysis identified 4144 tRNAs, 7537 rRNAs, 707 miRNAs, and 3400 snRNAs within the genome. Comparative genomics revealed that <em>N</em>. <em>jatamansi</em> exhibited high genetic redundancy. Phylogenetic analysis supports the current knowledge that the new APG IV classification system places <em>N</em>. <em>jatamansi</em> within the Caprifoliaceae family. Genes from the most expanded gene families, especially the TPS gene cluster at the end of chromosome 3A, are likely involved in the biosynthesis of sesquiterpenoids in <em>N. jatamansi</em>. Hopefully, our work would provide a valuable basis for revealing natural product-related biosynthetic pathways in <em>N. jatamansi</em> and advancing evolutionary studies within the Caprifoliaceae family.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114607"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity and mechanism of polyketides from the marine-derived fungus Talaromyces sp.","authors":"Ruiyun Huo ,&nbsp;Guangrong Zi ,&nbsp;Dongxiao Shi ,&nbsp;Ying Shi ,&nbsp;Jinwei Ren ,&nbsp;Kui Hong ,&nbsp;Ling Liu","doi":"10.1016/j.phytochem.2025.114606","DOIUrl":"10.1016/j.phytochem.2025.114606","url":null,"abstract":"<div><div>Six previously undescribed natural products, talaromenes A−D (<strong>1</strong>−<strong>4</strong>), prugosene F (<strong>5</strong>), and prugosene G (<strong>6</strong>), as well as four previously known compounds (<strong>7</strong>−<strong>10</strong>), were isolated and characterized from the culture of the marine-derived fungus <em>Talaromyces</em> sp. WHUF04072. The structures of these compounds were determined by the HRESIMS and NMR spectroscopic data. The absolute configurations of the unreported compounds were determined by ECD calculations and ECD experiments induced by Rh₂(OCOCF₃)₄. Moreover, all isolated compounds were evaluated for their antibacterial activities. Compound <strong>8</strong> exhibit significant antibacterial activities against several strains of <em>Staphylococcus aureus</em> with MICs ranging from 0.125 to 0.25 μg/mL. Additionally, it can effectively inhibit the biofilms formation of methicillin-resistant <em>S. aureus</em> (MRSA). Further mechanism studies revealed that <strong>8</strong> could exhibited significant antibacterial effects against MRSA by disrupting bacterial membranes. These results suggested that <strong>8</strong> may be developed into a new antibacterial candidate for combating MRSA infections.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114606"},"PeriodicalIF":3.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In memoriam of Professor Marie-Aleth Lacaille-Dubois 1950–2025","authors":"Anne-Claire Mitaine-Offer","doi":"10.1016/j.phytochem.2025.114593","DOIUrl":"10.1016/j.phytochem.2025.114593","url":null,"abstract":"","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"238 ","pages":"Article 114593"},"PeriodicalIF":3.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dammarane triterpenoids with anti-inflammatory and promoting glucose uptake activities from the leaves of Cyclocarya paliurus","authors":"Xiaoqin Liang ,&nbsp;Qingfang Zheng ,&nbsp;Biqun Zou ,&nbsp;Yanqin Li ,&nbsp;Liwei Pan ,&nbsp;Chenyang Ren ,&nbsp;Jiwen Xin ,&nbsp;Bo Yang ,&nbsp;Ruiyun Yang ,&nbsp;Weifeng Xu ,&nbsp;Chaowen He ,&nbsp;Yanling Cui ,&nbsp;Wen Tang ,&nbsp;Ruijie He ,&nbsp;Jun Li","doi":"10.1016/j.phytochem.2025.114605","DOIUrl":"10.1016/j.phytochem.2025.114605","url":null,"abstract":"<div><div>Fourteen triterpenoids, namely eight undescribed dammarane triterpenoid saponins (<strong>1</strong>–<strong>8</strong>, named cypaliurusides S–Z₇) and six known analogues (<strong>9</strong>–<strong>14</strong>), were isolated from the leaves of <em>Cyclocarya paliurus</em>. The undescribed compounds exhibited diverse side-chain modifications, including conjugated double bonds, multiple hydroxyl groups and a five-member oxygen ring formed via 20,24-diol dehydration. Their structures were elucidated through comprehensive nuclear magnetic resonance (NMR) (1D/2D) and high-resolution electrospray ionisation mass spectrometry data analysis, with absolute configurations determined by dimolybdenum tetraacetate induced <strong>circular dichroism</strong> spectra and <strong>density functional theory gauge-independent atomic orbital</strong> ¹³C-NMR calculation. <strong>Bioactivity evaluation</strong> revealed that cypaliurusides T (<strong>2</strong>) and U (<strong>3</strong>) exhibited <strong>potent</strong> anti-inflammatory activity [<strong>half-maximal inhibitory concentration (</strong>IC₅₀) = 7.6 and 8.1 μM, respectively], surpassing that of the positive control dexamethasone (IC₅₀ = 9.2 μM). Additionally, cypaliurusides S (<strong>1</strong>), T (<strong>2</strong>), U (<strong>3</strong>), X (<strong>5</strong>) and Y (<strong>6</strong>) <strong>showed a dose-dependent enhancement</strong> of insulin-stimulated glucose uptake in 3T3-L1 adipocytes at concentrations of 2.5–10 μM. Structure–activity relationship analysis indicated that C-24/C-25 dihydroxylation is critical for anti-inflammatory activity, while the C-25 hydroxyl group is essential for enhancing glucose uptake. These findings may contribute to the discovery of antidiabetic agents.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114605"},"PeriodicalIF":3.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eleven undescribed bisabolane-type sesquiterpenoids from Artemisia persica","authors":"Jakhongir Umaraliev ,&nbsp;Adkham Ganiev ,&nbsp;Ablajan Turak ,&nbsp;Nurmirza Begmatov ,&nbsp;Khayrulla Bobakulov ,&nbsp;Nasrulla Abdullaev ,&nbsp;Zhao Jiangyu ,&nbsp;Haji Akber Aisa","doi":"10.1016/j.phytochem.2025.114602","DOIUrl":"10.1016/j.phytochem.2025.114602","url":null,"abstract":"<div><div>Eleven previously undescribed bisabolane-type sesquiterpenoids were successfully isolated from <em>Artemisia persica</em>. The structures of these compounds were comprehensively elucidated through a combination of advanced spectroscopic techniques, including one-dimensional and two-dimensional nuclear magnetic resonance, high-resolution electrospray ionization mass spectrometry, and ultraviolet spectroscopy. Structural assignments were further confirmed by comparison of experimental and time-dependent density functional theory-calculated electronic circular dichroism spectra, as well as single-crystal X-ray diffraction analysis where applicable. To assess their pharmacological relevance, the isolated sesquiterpenoids were subjected to <em>in vitro</em> evaluation of anti-inflammatory activity and cytotoxic potential. Compounds 6 and 9 demonstrated limited anti-inflammatory activity in the lipopolysaccharide-induced inflammation model using RAW 264.7 mouse macrophages, with IC₅₀ values of 111.1 ± 4.66 μM and 247.8 ± 1.49 μM, respectively.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114602"},"PeriodicalIF":3.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of a previously undescribed immunopotentiator: identification and immune-stimulating activity of saponins from Hylomecon japonica (Thunb.) Prantl & Kündig","authors":"Peixin Shi,&nbsp;Jingjing Zhao,&nbsp;Jianan Niu,&nbsp;Jianxiu Zhai,&nbsp;Jun Yin","doi":"10.1016/j.phytochem.2025.114601","DOIUrl":"10.1016/j.phytochem.2025.114601","url":null,"abstract":"<div><div>The total saponins from <em>Hylomecon japonica</em> (Thunb.) Prantl &amp; Kündig were found to exhibit significant immune-stimulating activity. This finding prompted the bioassay-guided isolation of six previously undescribed saponins (<strong>1</strong>–<strong>6</strong>) from the herb. Their chemical structures were unambiguously determined through comprehensive spectroscopic data analysis. Initial screening revealed that compounds <strong>1</strong>–<strong>3</strong> did not exhibit cytotoxic effects on splenic lymphocytes <em>in vitro</em>, and compound <strong>2</strong> demonstrated significant proliferative activity. Further, flow cytometry analysis showed that compound <strong>2</strong> significantly increased the expression levels of CD80, CD86, and MHC II on the surface of bone marrow-derived dendritic cells (BMDCs), suggesting its potential to promote BMDCs maturation. Moreover, ELISA and RT-qPCR assays indicated that compound <strong>2</strong> enhanced the production of immune cytokines IFN-γ, IL-2, and TNF-α, and upregulated the expression of immune-related genes IFN-γ, NF-κB, and TLR4 in BMDCs. Additionally, mixed lymphocyte reaction (MLR) experiments confirmed that compound <strong>2</strong> amplified the T cell-mediated immune response facilitated by BMDCs. These findings establish a fundamental basis for investigating immune-enhancing effect of triterpenoids.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114601"},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural products against autoimmune hepatitis: Structures, pharmacologies and mechanisms","authors":"Jiahui Wang ,&nbsp;Qianru Wang ,&nbsp;Wanxin Xiao,&nbsp;Chenyu Dang,&nbsp;Shuanggang Ma","doi":"10.1016/j.phytochem.2025.114603","DOIUrl":"10.1016/j.phytochem.2025.114603","url":null,"abstract":"<div><div>Autoimmune hepatitis (AIH) is a chronic disease characterized by lymphocyte infiltration in the liver. Ignoring and delaying treatment may lead to cirrhosis or end-stage liver failure. The incidence and prevalence of AIH are gradually increasing worldwide, affecting individuals of all ages, with females being particularly susceptible. Although a prompt response to treatment with corticosteroids and other immunomodulatory drugs is nearly universal and supports the diagnosis, their side effects cannot be avoided. Chinese herbs have been used for thousands of years to treat a wide range of diseases. Natural products (NPs) have become vital resources for bioactive agents in the discovery of anti-AIH drugs. Recently, more natural components have been utilized for treating liver disorders, emerging as a promising therapy for various hepatic conditions. The structures and properties of natural products for treating AIH, including phytochemistry, potential therapeutic mechanisms, and recent advances are summarized in this review. The development of novel anti-AIH agents will be significantly advanced by confirming the effective inhibition of AIH by NPs both <em>in vitro</em> and <em>in vivo</em>.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114603"},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous nitric oxide alleviates the toxicity of 1,3-dibutyl imidazole bromide on foxtail millet","authors":"Dongsheng Wang,&nbsp;Yueyue Qiao,&nbsp;Weizhong Liu","doi":"10.1016/j.phytochem.2025.114604","DOIUrl":"10.1016/j.phytochem.2025.114604","url":null,"abstract":"<div><div>Ionic liquids exhibit distinctive properties, including low vapor pressure, high thermal stability, good electrical conductivity, and versatile design possibilities, which render them applicable across various fields. However, their increasing utilization has raised concerns regarding ecotoxicity. This study investigates the impact of 1,3-dibutyl imidazole bromide (DIB) on foxtail millet and examines the effects of exogenously applied sodium nitroprusside, a nitric oxide donor, on the toxicity of DIB. Physiological analyses indicated that DIB treatment inhibits seed germination and seedling growth, and decreases chlorophyll content; conversely, malondialdehyde levels were notably elevated in foxtail millet. The exogenously applied nitric oxide mitigated these detrimental effects. Nitric oxide also enhanced peroxidase activity and increased the concentrations of two osmotic regulation compounds, proline and soluble sugars, in leaves of foxtail millet. RNA sequencing results suggested that nitric oxide alleviates DIB-induced phytotoxicity possibly through modulation of chlorophyll metabolism, biosynthesis pathways for various secondary metabolites, signaling transduction involving ethylene and jasmonate responses, as well as processes related to metal ion binding. These investigations not only elucidate the specific impacts of ionic liquids on plant physiology but also provide a scientific basis for employing exogenous nitric oxide as an effective strategy for mitigating toxicity in agricultural practices.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114604"},"PeriodicalIF":3.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}