Phytochemistry最新文献

{"title":"HPLC-UV guided isolation of anti-inflammatory polyacetylene glucosides from Carthamus tinctorius L.","authors":"Yong-Xiang Wang ,&nbsp;Jia-Xu Bao ,&nbsp;Ran Wang ,&nbsp;Xian Zhang ,&nbsp;Yun-Fang Zhao ,&nbsp;Peng-Fei Tu ,&nbsp;Hui-Xia Huo ,&nbsp;Jun Li","doi":"10.1016/j.phytochem.2025.114629","DOIUrl":"10.1016/j.phytochem.2025.114629","url":null,"abstract":"<div><div>HPLC-UV guided fractionation led to the isolation of nineteen previously undescribed polyacetylene glucosides (<strong>1</strong>–<strong>19</strong>) from the florets of <em>Carthamus tinctorius</em> L. Their structures, including the absolute configurations, were established by analysis of UV, IR, HRESIMS, 1D and 2D NMR data, together with enzymatic hydrolysis and ECD calculations. Compounds <strong>1</strong> and <strong>2</strong> were polyacetylene glucosides bearing an unusual furan ring. Compounds <strong>3</strong>–<strong>6</strong> were rare polyacetylene glucosides with an <em>α</em>-<span><em>l</em></span>-arabinofuranosyl moiety, while compound <strong>3</strong> comprised a 1-O-<em>α</em>-<span><em>l</em></span>-arabinofuranosyl-(1 → 3)-<em>β</em>-<span><em>d</em></span>-glucopyranosyl disaccharide unit. Compounds <strong>2</strong>, <strong>4</strong>, <strong>5</strong>, <strong>8</strong>, <strong>11</strong>–<strong>13</strong>, <strong>16</strong>, and <strong>18</strong> showed inhibition of nitric oxide secretion in lipopolysaccharide-stimulated RAW264.7 cells with IC₅₀ values ranging from 5.0 to 40.0 μM.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114629"},"PeriodicalIF":3.4,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive constituents from Salacia obovatilimba as potential in vitro hSGLT2 inhibitors","authors":"Zhi-Yong Jiang ,&nbsp;Lin Chen ,&nbsp;Hong-Xi Xie ,&nbsp;Jun-Jie Hao ,&nbsp;Rong Chen ,&nbsp;Chun-Qiu Huang ,&nbsp;Hong-Lei Zhang ,&nbsp;Ai-Xue Zuo","doi":"10.1016/j.phytochem.2025.114626","DOIUrl":"10.1016/j.phytochem.2025.114626","url":null,"abstract":"<div><div>Sixteen compounds including twelve previously undescribed sesquiterpenes were isolated from <em>Salacia obovatilimba</em>. Based on HRESIMS, IR, NMR and electronic circular dichroism (ECD) spectroscopic analysis, the previously undescribed compounds were characterized as twelve undescribed dihydro-<em>β</em>-agarofuran sesquiterpenoids (compounds <strong>1</strong>–<strong>12</strong>). The structures of compounds <strong>1</strong> and <strong>6</strong> were confirmed by single-crystal X-ray diffraction. All compounds were assayed for their inhibitory effect on <em>h</em>SGLT2 in vitro, results suggested compounds <strong>11</strong> and <strong>13</strong> exhibited significant <em>h</em>SGLT2 inhibitory activity, with IC₅₀ values of 11.9 and 9.45 nM, respectively. The probable binding potency were predicted through molecular docking by accurate models of protein-ligand complex.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114626"},"PeriodicalIF":3.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defence induction and identification of key genes associated with Lymantria dispar oral secretions in Populus davidiana × P. bolleana on the basis of transcriptome and hormone levels","authors":"Yuan Gao ,&nbsp;Mengyuan Wang ,&nbsp;Qingjun Xie ,&nbsp;Lili Sun ,&nbsp;Chuanwang Cao","doi":"10.1016/j.phytochem.2025.114625","DOIUrl":"10.1016/j.phytochem.2025.114625","url":null,"abstract":"<div><div>In the constant battle with herbivorous insects, plants have evolved sophisticated recognition and adaptation mechanisms to fight insect attacks. Insect infestation is a major challenge in poplar afforestation production. In this study, we mimicked insect feeding by applying insect oral secretions (OS) to the leaf mechanical wounds of <em>Populus davidiana</em> × <em>P. bolleana</em> and combined transcriptome and hormone analyses to investigate the effects of OS from <em>Lymantria dispar</em> on defence responses and to identify the key genes involved in <em>Populus davidiana</em> × <em>P. bolleana</em> defence. Our results revealed that 3067 differentially expressed genes were involved in the OS-induced response to wounding in <em>Populus davidiana</em> × <em>P. bolleana</em>. OS treatment can rapidly induce the expression of genes related to the jasmonic acid (JA) biosynthetic and signal transduction pathways. The contents of JA, jasmonoyl-<span>l</span>-isoleucine, indole-3-acetic acid and salicylic acid significantly increased in response to OS. To construct a coexpression gene network, a hub gene annotated as <em>WRKY22</em> was identified in <em>Populus davidiana</em> × <em>P. bolleana.</em> To investigate the function of the <em>PdbWRKY22</em> gene against pests, <em>PdbWRKY22</em> was successfully transformed into <em>Populus davidiana × P. bolleana.</em> Transient overexpression of <em>PdbWRKY22</em> induced JA-related gene expression and suppressed the feeding behaviour of <em>L. dispar</em> larvae under OS treatment. These results suggested the defence mechanism of <em>Populus davidiana</em> × <em>P. bolleana</em> against <em>L. dispar</em> at the transcriptional and hormonal levels, and revealed that <em>PdbWRKY22</em> plays a crucial physiological role in <em>Populus davidiana</em> × <em>P. bolleana</em>.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114625"},"PeriodicalIF":3.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cassane diterpenoids with hepatic gluconeogenesis inhibitory activity from the seeds of Caesalpinia decapetala (Roth) Alston","authors":"De-Sheng Wang ,&nbsp;Yu Shen ,&nbsp;Lin-Fen Ding ,&nbsp;Run-Cheng Yan ,&nbsp;Ming Hu ,&nbsp;Wen-Chao Tu ,&nbsp;Liu-Dong Song ,&nbsp;Suling Huang ,&nbsp;Xing-De Wu","doi":"10.1016/j.phytochem.2025.114628","DOIUrl":"10.1016/j.phytochem.2025.114628","url":null,"abstract":"<div><div>A chemical investigation of the seeds of <em>Caesalpinia decapetala</em> (Roth) Alston led to the isolation of fifteen cassane diterpenoids, including ten previously undescribed compounds (<strong>1</strong>–<strong>10</strong>) and five known analogues (<strong>11</strong>–<strong>15</strong>). The structures of <strong>1</strong>–<strong>10</strong> were determined using ¹H, ¹³C, and 2D NMR, HRESIMS, and IR data. Their absolute configurations were confirmed by comparing experimental electronic circular dichroism data with calculated values, coupled with single-crystal X-ray diffraction analysis. Structurally, compounds <strong>1</strong> and <strong>2</strong> are rare cassane diterpenoids featuring a ketone carbonyl group at C-3. Excessive hepatic gluconeogenesis plays an important role in the occurrence and development of type 2 diabetes mellitus. In the present study, compounds <strong>1</strong>, <strong>3</strong>, <strong>4</strong>, <strong>6</strong>, <strong>11</strong>, and <strong>12</strong> exhibited potent inhibitory effects on gluconeogenesis at a concentration of 20 μM in primary mouse hepatocytes, with inhibitory capacities of 68.2 ± 6.8 %, 78.4 ± 3.5 %, 53.8 ± 6.1 %, 94.8 ± 5.5 %, 85.1 ± 4.1 %, and 106.9 ± 2.9 %, respectively. Further studies indicated that these compounds inhibited gluconeogenesis in a dose-dependent manner across a concentration range from 1 to 50 μM. Notably, compounds <strong>6</strong> and <strong>12</strong> exhibited potent inhibitory effects on gluconeogenesis at a concentration of 2 μM, comparable to the effects of 500 μM metformin, suggesting their potential for ameliorating hyperglycemia.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114628"},"PeriodicalIF":3.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of rearranged eudesmane-type sesquiterpenes from Artemisia tournefortiana","authors":"Guziliayi Kuerban ,&nbsp;Ablajan Turak ,&nbsp;Nannan Xu ,&nbsp;Dan Tang ,&nbsp;Deng Zang ,&nbsp;Xiayidan Abulimiti ,&nbsp;Jiangyu Zhao ,&nbsp;Haji Akber Aisa","doi":"10.1016/j.phytochem.2025.114627","DOIUrl":"10.1016/j.phytochem.2025.114627","url":null,"abstract":"<div><div>Six undescribed sesquiterpenes artetourneforin F–K (<strong>1</strong>–<strong>6</strong>), along with six known compounds (<strong>7</strong>–<strong>12</strong>), were isolated from <em>Artemisia tournefortiana</em>. Their structures were established by 1D and 2D NMR spectroscopic data, HRESIMS, ECD, and quantum chemical calculations. Artetourneforin F–K (<strong>1</strong>–<strong>6</strong>) represented a rare 4,5-<em>seco</em> undescribed skeleton in natural sesquiterpenes, and a plausible biosynthetic pathway was proposed. The X-ray crystal structures of compounds <strong>7</strong> and <strong>8</strong> were reported for the first time. Compound <strong>9</strong> showed extremely significant anti-inflammatory and cytotoxicity activity against HeLa cell lines with IC₅₀ values of 0.87 ± 0.05 μM and 4.02 ± 0.15 μM, respectively. In the anti-vitiligo activity test, compounds <strong>8</strong> and <strong>10</strong> demonstrated activity in promoting melanogenesis in B16F10 cells. Compound <strong>8</strong> exhibited significant cytotoxicity against the HT29 and MCF7 cell lines, with IC₅₀ values of 6.40 ± 0.41 and 10.18 ± 0.49 μM, respectively.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114627"},"PeriodicalIF":3.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical profiling of Bai traditional food: Eight previously undescribed meroterpenoids derivatives with cytotoxic potential from the flowers of Rhododendron pachypodum","authors":"Ying Wang ,&nbsp;Jie Luan ,&nbsp;Ji-Ai Wang ,&nbsp;Xiu Chen ,&nbsp;Fu-Cai Ren ,&nbsp;Bing Chen ,&nbsp;Jing Jia ,&nbsp;Gao-Hong Zhang ,&nbsp;Yong Li ,&nbsp;Jiang-Bo He","doi":"10.1016/j.phytochem.2025.114623","DOIUrl":"10.1016/j.phytochem.2025.114623","url":null,"abstract":"<div><div>The flowers of <em>Rhododendron pachypodum</em> (Ericaceae), a traditional edible vegetable of the Bai ethnic group in Yunnan Province, China, are widely used both as food and medicine. A systematic phytochemical survey was conducted to fill the knowledge gap regarding its bioactive constituents. Fourteen meroterpenoids, including eight previously undescribed meroterpenoid compounds, rubiginosin I–J (<strong>1</strong>–<strong>2</strong>), (±)-rubiginosin K (<strong>3</strong>), (±)-rubiginosin L (<strong>4</strong>), (±)-rubiginosin N (<strong>5</strong>), and six known analogues (<strong>6</strong>–<strong>11</strong>), were structurally elucidated using a combination of spectroscopic techniques (1D/2D NMR, HRESIMS, UV, IR). The absolute configurations were unambiguously established by X-ray crystallography and ECD calculations. ‌Selected compounds were assessed for their cytotoxicity, <em>α</em>-glucosidase inhibition, and DPPH radical scavenging activities. Compound <strong>6</strong> demonstrated cytotoxic effects against‌ HepG2 and HeLa cell lines, with IC₅₀ values of 40.56 μM and 57.43 μM, respectively. Compound <strong>7</strong> exhibited a slight cytotoxic activity against A549 cell lines, with an inhibition rate of 60 %, while compound <strong>8</strong> demonstrated mild activity against both A549 and MDA-MB-468 cells, with inhibition rates of 70 % and 65 %, respectively, at a concentration of 100 μM. In the α-glucosidase inhibition assay, compounds <strong>2</strong>–<strong>11</strong> showed weak activity at 100 μg/mL. Similarly, in the DPPH assay, these compounds exhibited poor free radical scavenging activity at 100 μg/mL. This study not only expands the chemical diversity of meroterpenoids in <em>Rhododendron pachypodum</em>, but also provides the first evidence of their cytotoxic potential, although structural optimization is required to enhance bioactivity.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114623"},"PeriodicalIF":3.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C-glycosylated and alkylated flavonoids with potent anti-inflammatory activity from the whole plants of Drymaria cordata","authors":"Lan-Yi Lu ,&nbsp;Yan-Hua Wu ,&nbsp;Peng Zhang ,&nbsp;Li-Yan Wang","doi":"10.1016/j.phytochem.2025.114624","DOIUrl":"10.1016/j.phytochem.2025.114624","url":null,"abstract":"<div><div><em>Drymaria cordata</em> is a traditional Chinese medicinal plant used for the treatment of hepatitis and chronic nephritis. Six previously undescribed flavonoid C-glycosides (<strong>1</strong>–<strong>3</strong>, <strong>8</strong>, <strong>9</strong>, <strong>11</strong>), two unreported alkylated flavonoids (<strong>4</strong>, <strong>5</strong>), and four known flavonoids (<strong>6</strong>, <strong>7</strong>, <strong>10</strong>, <strong>12</strong>) were isolated from its 70 % ethanol extract. The structures were established by extensive analysis of NMR, HRMS, and X-ray single-crystal diffraction. All compounds exhibited inhibitory activity against nitric oxide production, with compound <strong>8</strong>, which contains a rare sugar moiety (3,5-dihydroxy-6-methyl-<em>γ</em>-pyrone), showing the most pronounced effect. Further investigation revealed that <strong>8</strong> suppressed the expression of several key cytokines and inhibited the phosphorylation of <em>p</em>65. Molecular docking studies indicated that <strong>8</strong> targeted IKK, an upstream regulator of the NF-κB pathway, thereby blocking its activation.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114624"},"PeriodicalIF":3.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical investigation and evaluation of the antioxidant activity of Cornus sanguinea","authors":"Annunziata Paolillo ,&nbsp;Antonietta Cerulli ,&nbsp;Roberta Cuozzo ,&nbsp;Francesco Sottile ,&nbsp;Francesco Montella ,&nbsp;Elena Ciaglia ,&nbsp;Sonia Piacente ,&nbsp;Milena Masullo","doi":"10.1016/j.phytochem.2025.114622","DOIUrl":"10.1016/j.phytochem.2025.114622","url":null,"abstract":"<div><div><em>Cornus sanguinea</em> L. (common dogwood) is a small tree with leaves showing a characteristic dark red colour in senescence. <em>C. sanguinea</em> is traditionally used for several diseases. The drupes are used as a remedy for diarrhea, stomachaches, sore eyes, sore throats, and gastrointestinal disorders, and the dried roots are used as a febrifuge. With the aim to further define the chemistry of <em>C. sanguinea</em> and to validate its biological activities, the methanol extract of the whole plant was investigated. The total phenolic content and the total flavonoid content of <em>C. sanguinea</em> extract were evaluated along with the antioxidant activity by TEAC and DPPH assays. To determine the <em>in vitro</em> antioxidant activity, ROS inhibition analysis was performed by DHR assay on HaCat keratinocytes cell line pre-treated with the extract. The extract displayed statistically significant activity in the range 32–125 μg/mL in the HaCat cell line stimulated with 2 mM H₂O₂. The chemical profile of the extract was investigated by an initial LC-MS analysis that guided the identification and isolation of 25 compounds, of which the structures were unambiguously elucidated by NMR analysis and LC-MS analysis as phenolic acids, flavonoids, megastigmanes, and benzofuran. This study yielded four previously unreported compounds: one megastigmane derivative and three phenolic acids. Additionally, two oxylipins were tentatively identified by LC-MS.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114622"},"PeriodicalIF":3.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory cyclopropanes and benzofurans from mangrove endophytic fungus Schizophyllum sp. QYM-12","authors":"Tao Chen ,&nbsp;Bing Sun ,&nbsp;Xingze Hu ,&nbsp;Yanghui Ou ,&nbsp;Jianying Wu ,&nbsp;Tingshun Zhu ,&nbsp;Hongliang Yao ,&nbsp;Zhigang She","doi":"10.1016/j.phytochem.2025.114611","DOIUrl":"10.1016/j.phytochem.2025.114611","url":null,"abstract":"<div><div>Ten undescribed metabolites, including seven cyclopropane derivatives (<strong>1</strong>−<strong>7</strong>) and three benzofuran derivatives (<strong>11</strong>−<strong>13</strong>), along with three known cyclopropane analogues (<strong>8</strong>−<strong>10</strong>) were isolated from the mangrove endophytic fungus <em>Schizophyllum</em> sp. QYM-12. Their planar structures were established through mass spectrometric and extensive spectroscopic data. The absolute configurations of all compounds were determined by the modified Mosher's method, electronic circular dichroism calculations, ¹³C NMR calculations and DP4+ analysis. In the bioassay, compounds <strong>6</strong> and <strong>9</strong> exhibited IL-6 inhibitory activities with IC₅₀ values of 34.5 and 25.4 μM, respectively.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"240 ","pages":"Article 114611"},"PeriodicalIF":3.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyano-containing Daphniphyllum alkaloids with antienteroviral activity from the leaves and stems of Daphniphyllum calycinum Benth","authors":"Peishan Gu ,&nbsp;Yang Jin ,&nbsp;Shujie Jia ,&nbsp;Xiao-Nian Li ,&nbsp;Yue Liu ,&nbsp;Yanheng Zhou ,&nbsp;Meixin Chen ,&nbsp;Yongbo Xue","doi":"10.1016/j.phytochem.2025.114610","DOIUrl":"10.1016/j.phytochem.2025.114610","url":null,"abstract":"<div><div>Enterovirus 71 (EV71), a major causative agent of hand, foot, and mouth disease (HFMD), is often associated with severe clinical outcomes. In this study, three rare cyano-containing alkaloids, cyanodaphcalycines A–C (<strong>1</strong>–<strong>3</strong>), featuring an unusual 5/6/6/7/5/5-hexacyclic scaffold, and an undescribed daphmanidin A-type alkaloid, daphmanidin G (<strong>4</strong>), were isolated from the leaves and stems of the medicinal plant <em>Daphniphyllum calycinum</em> Benth., along with three known <em>Daphniphyllum</em> alkaloids (<strong>5</strong>–<strong>7</strong>). The previously undescribed structures and their absolute configurations were determined using a combination of spectroscopic analysis, a single crystal X-ray diffraction analysis, and time-dependent density functional theory-electronic circular dichroism (TDDFT-ECD) calculations. In the biological evaluation, the antiviral activities of compounds <strong>1</strong>–<strong>4</strong> were tested against EV71 in human malignant embryonal rhabdomyosarcoma (RD) cells. Compounds <strong>3</strong> and <strong>4</strong> exhibited potent antiviral effects against human EV71 in RD cells, with EC₅₀ values of 3.78 ± 0.23 and 6.87 ± 0.30 μg/mL, respectively, demonstrating superior efficacy to ribavirin (EC₅₀ 65.77 ± 0.11 μg/mL). This is the first report of <em>Daphniphyllum</em> alkaloids as potent inhibitors of EV71.</div></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":"239 ","pages":"Article 114610"},"PeriodicalIF":3.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}